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Article in Chinese | WPRIM | ID: wpr-878931


This paper was to investigate the effect of Huanglian Jiedu Decoction(HLJD) on ulcerative colitis(UC) in mice, and determine the effective components in plasma, and virtually screen its therapeutic target, and predict its mechanism. Sixty Balb/c mice were randomly divided into blank group, model group, mesalazine treatment group(0.3 g·kg~(-1)), and HLJD treatment groups(24.66, 12.33, 6.17 g·kg~(-1)). Excepted for the blank group, all the mice in HLJD and mesalazine treatment groups were gavage administration. All mice freely drank 2.5% DSS solution for seven days to induce UC. The disease activity index(DAI) was detected each day. At the end of the experiment, HE staining was used to observe the pathological changes in colon. The content of IL-1β, IL-6 and TNF-α in colon were determined by ELISA. The effective components in plasma were determined by UPLC-Q-TOF-MS. The reverse docking in PharmMapper was used to screen the component targets. The disease targets of UC were collected by searching TTD, OMIM and GeneCards databases. The intersection of the component targets and disease targets was selected as the therapeutic targets. Then the therapeutic targets were imported into the STRING for GO and KEGG enrichment analysis. Discovery Studio was used to simulate the docking between the components and the targets. RESULTS:: showed that the DAI in the model group increased significantly(P<0.05), and the number of inflammatory cells and infiltration degree increased significantly compared with the blank group. The DAI in HLJD treatment group was significantly reduced(P<0.05), and the number and infiltration degree of inflammatory cells were reduced compared with the model group. The ELISA results showed that the levels of IL-1β, IL-6 and TNF-α were increased significantly in the model group(P<0.01) compared with the blank group, and significantly down regulated in the HLJD treatment group(P<0.05) compared with the model group. After UPLC-Q-TOF-MS analyse, ten components were identified. The network pharmacology analysis showed that the action targets were significantly enriched in 129 of biological processes, such as response to organic substance, chemical and oxygen-containing compound, etc., as well as 16 of signal pathways, such as IL-17, TNF and hepatitis B signal pathways, were enriched too. The results of molecular docking showed that limonin, palmatine and berberine could bind to CASP3 and MMP9 by hydrogen bond. In conclusion, HLJD could alleviate the colonic mucosal inflammatory infiltration and mucosal damage in UC mice. The mechanism may be related to the anti-inflammatory effect on UC mice by reducing the levels of IL-1β, IL-6 and TNF-α in colon through limonin, palmatine and berberine regulating IL-17 signal pathway and TNF signal pathway via CASP3 and MMP9 meditated.

Animals , Anti-Inflammatory Agents/therapeutic use , Colitis, Ulcerative/drug therapy , Colon , Dextran Sulfate/therapeutic use , Drugs, Chinese Herbal , Mice , Molecular Docking Simulation , Plasma
Protein & Cell ; (12): 489-500, 2017.
Article in English | WPRIM | ID: wpr-757008


Innate lymphoid cells (ILCs) are a newly classified family of immune cells of the lymphoid lineage. While they could be found in both lymphoid organs and non-lymphoid tissues, ILCs are preferentially enriched in barrier tissues such as the skin, intestine, and lung where they could play important roles in maintenance of tissue integrity and function and protection against assaults of foreign agents. On the other hand, dysregulated activation of ILCs could contribute to tissue inflammatory diseases. In spite of recent progress towards understanding roles of ILCs in the health and disease, mechanisms regulating specific establishment, activation, and function of ILCs in barrier tissues are still poorly understood. We herein review the up-to-date understanding of tissue-specific relevance of ILCs. Particularly we will focus on resident ILCs of the skin, the outmost barrier tissue critical in protection against various foreign hazardous agents and maintenance of thermal and water balance. In addition, we will discuss remaining outstanding questions yet to be addressed.

Animals , Humans , Immunity, Innate , Physiology , Inflammation , Allergy and Immunology , Lymphocytes , Allergy and Immunology , Skin , Allergy and Immunology
Article in Chinese | WPRIM | ID: wpr-233833


<p><b>OBJECTIVE</b>To evaluate the effect of peer education on postoperative rehabilitation of patients with laryngeal cancer.</p><p><b>METHODS</b>From January 2010 to December 2012, 240 cases of primary laryngeal cancer were treated with partial or total laryngectomy as well as tracheotomy in the Department of Head and Neck Surgery in Shandong Provincial Hospital. The patients were randomly divided into experimental group or control group by Stochastic tables law, 120 patients in control group accepted only conventional nursing, 120 patients in experimental group accepted both conventional nursing and peer education. The difference in the treatment nursing cooperation attitude, postoperative recovery, postoperative symptoms and the incidence of complications between two groups were evaluated by postoperative quality of life (UW-QOL), Eysenck personality questionnaire (EPQ) and coping style questionnaire (CSQ) score. The independent sample T-test and χ² test were used for statistical analysis.</p><p><b>RESULTS</b>The patients of experimental group showed more positive attitude to treatment and care compared to the patients of control group (χ² = 10.7, P < 0.01). Experimental group had less time in postoperative wound healing time, gastric tube indwelling time and postoperative hospital stay than control group, with statistically significant differences (t = 6.89, t = 6.36, t = 6.42, respectively, P < 0.01); Incidence of postoperative discomfort symptoms in experimental group was less than that in control group (P < 0.05). Total score for quality of life (UW-QOL) in experimental group was higher than that in control group (t = 2.40, P < 0.05). The P and N scores of EPQ in experimental group were lower than those in control group (t = 2.28, t = 2.60, P < 0.05), while the E score of EPQ in experimental group was significantly higher than that in control group (t = 4.50, P < 0.01), with no significant difference in the L score of EPQ between two groups (P > 0.05). scores of factor to solve problem and help factor of CSQ in experimental group were higher than those in control group (t = 7.05, t = 7.73, P < 0.01), and the era factor score of experimental group was lower than that of control group (t = 2.05, P < 0.05).</p><p><b>CONCLUSIONS</b>Peer education can help the patients with laryngectomy to maintain good psychological and mental status, to cooperate actively with treatment, to reduce symptoms and complications, and to shorten the length of hospital stay, improving their postoperative rehabilitation and quality of life.</p>

Health Knowledge, Attitudes, Practice , Humans , Laryngeal Neoplasms , Rehabilitation , General Surgery , Laryngectomy , Peer Group , Postoperative Period , Quality of Life , Surveys and Questionnaires
Article in Chinese | WPRIM | ID: wpr-440924


Objective Seek countermeasures against the debt risk in universities. Methods Taking the debts of Kunming Chenggong universities construction as an example, analyze the reasons and countermeasures for the debts of universities in Yunnan province. Results Due to the enrollment expansion policy, the higher education in Yunnan province has been transformed from the elite education into mass education. The universities in Kunming have gained unprecedented development opportunities,especially with the completion of the Kunming Chenggong University Town. However, loan funds for the new campus construction mostly came from banks. So,the huge debt stress from banks has been brought with the development of the universities. Conclusion The sustainable development of universities requires the moderate debt. The government, universities and society need to work together to control the scale of debt effectively and avoid the debt crisis.

Protein & Cell ; (12): 571-580, 2012.
Article in English | WPRIM | ID: wpr-757257


Epithelial tissues covering the external and internal surface of a body are constantly under physical, chemical or biological assaults. To protect the epithelial tissues and maintain their homeostasis, multiple layers of immune defense mechanisms are required. Besides the epithelial tissue-resident immune cells that provide the first line of defense, circulating immune cells are also recruited into the local tissues in response to challenges. Chemokines and chemokine receptors regulate tissue-specific migration, maintenance and functions of immune cells. Among them, chemokine receptor CCR10 and its ligands chemokines CCL27 and CCL28 are uniquely involved in the epithelial immunity. CCL27 is expressed predominantly in the skin by keratinocytes while CCL28 is expressed by epithelial cells of various mucosal tissues. CCR10 is expressed by various subsets of innate-like T cells that are programmed to localize to the skin during their developmental processes in the thymus. Circulating T cells might be imprinted by skin-associated antigen- presenting cells to express CCR10 for their recruitment to the skin during the local immune response. On the other hand, IgA antibody-producing B cells generated in mucosa-associated lymphoid tissues express CCR10 for their migration and maintenance at mucosal sites. Increasing evidence also found that CCR10/ligands are involved in regulation of other immune cells in epithelial immunity and are frequently exploited by epithelium-localizing or -originated cancer cells for their survival, proliferation and evasion from immune surveillance. Herein, we review current knowledge on roles of CCR10/ligands in regulation of epithelial immunity and diseases and speculate on related important questions worth further investigation.

B-Lymphocytes , Cell Biology , Allergy and Immunology , Cell Lineage , Cell Movement , Genetics , Allergy and Immunology , Chemokine CCL27 , Genetics , Allergy and Immunology , Chemokines, CC , Genetics , Allergy and Immunology , Epithelial Cells , Cell Biology , Allergy and Immunology , Epithelium , Allergy and Immunology , Gene Expression Regulation , Allergy and Immunology , Humans , Immunity, Mucosal , Immunoglobulin A , Allergy and Immunology , Mucous Membrane , Cell Biology , Allergy and Immunology , Receptors, CCR10 , Genetics , Allergy and Immunology , Signal Transduction , Genetics , Allergy and Immunology , T-Lymphocytes , Cell Biology , Allergy and Immunology