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1.
Article in Chinese | WPRIM | ID: wpr-885352

ABSTRACT

Objective:To explore the effect of cognitive-behavioral therapy on psychological stress and quality of life in patients with pulmonary tuberculosis.Methods:According to two-level cluster random design 461 patients with tuberculosis from 20 communities in Pizhou county of Jiangsu province were selected in the study from September 2018 to November 2018. The intervention group received cognitive-behavioral therapy for two months, while control group received routine follow-up. Anxiety, depression and quality of life were assessed by GAD-7, PHQ-9 and SF-36 scales, respectively. At the same time, the comparison between the two groups was conducted by independent sample t test, and the difference between the two groups before and after treatment was analyzed by paired sample ttest. Results:A total of 454 participants were finally included in this analysis; there were 230 cases in the intervention group and 224 cases in the control group. In the intervention group the scores of anxiety and depression after intervention were significantly lower than the baseline scores [(7.57±5.27) vs. (5.93±2.56), t=-4.245, P<0.01; (8.13±6.01) vs. (6.02±2.67); t=-4.866, P<0.01], and the quality of life score was significantly higher than the baseline score [(58.46±12.71) vs. (74.31±13.22); t=13.108, P<0.01]; while in the control group there were no significant differences in the scores of anxiety, depression and quality of life after intervention, compared with those at baseline [(7.62±5.41) vs.(7.65±5.38); (8.00±5.84) vs. (8.07±5.91); (59.11±13.25) vs. (60.51±13.76); t=0.059, t=0.126, t=1.104, all P>0.05]. However, only for patients with mild and moderate anxiety and depression symptoms in the intervention group, the anxiety and depression scores were decreased after intervention [(7.29±1.21) vs. (5.54±1.71), (11.99±1.31) vs. (9.17±1.55); (7.01±1.47) vs. (4.42±1.22), (11.88±1.12) vs. (8.39±2.33); t=8.056, t=10.020, t=13.558, t=8.852,all P<0.01]. Conclusion:Cognitive-behavioral therapy can relieve the psychological pressure and improve the quality of life in pulmonary tuberculosis patients with mild or moderate anxiety/depression symptoms.

2.
Article in Chinese | WPRIM | ID: wpr-879430

ABSTRACT

OBJECTIVE@#To observe the analgesic effect of manipulation loading on chronic low back pain (CLBP) model rats and the expression of inflammatory factors in psoas major muscle tissue, and to explore the improvement of manipulation on local inflammatory microenvironment.@*METHODS@#Thirty two SPF male SD rats weighing 340-360g were randomly divided into blank group, sham operation group, chronic low back pain model group and treatment group, with 8 rats in each group. In the model group, L@*RESULTS@#There was no significant difference in PWT and PWL between the blank group and the sham operation group after modeling (@*CONCLUSION@#Local massage loading has analgesic effect on CLBP rats, at the same time, it can inhibit the content of CGRP and NGF in psoas muscle tissue of CLBP rats, and improve the local inflammatory microenvironment.


Subject(s)
Animals , Calcitonin , Calcitonin Gene-Related Peptide , Low Back Pain/therapy , Male , Nerve Growth Factor/genetics , Rats , Rats, Sprague-Dawley
3.
Article in Chinese | WPRIM | ID: wpr-878976

ABSTRACT

Breast tumor has become one of the malignant tumors with the highest incidence, and is a serious threat to human health, especially to women. Chemotherapy is an important anti-breast tumor therapy, which can be used in almost every stage of breast tumor therapy alone or in the combination with surgery and radiation therapy. Alkaloids are a kind of ubiquitous natural products, and important active components of various medicinal plants. A large number of studies have shown that alkaloids could exert an anti-breast tumor effect by inhibiting proliferation, metastasis and angiogenesis, resisting mitosis, promoting apoptosis and autophagy, and triggering cell cycle arrest. The extensive anti-breast tumor effect makes alkaloids an important candidate drug source. This paper reviews the anti-breast tumor mechanism of natural products of alkaloids.


Subject(s)
Alkaloids/pharmacology , Apoptosis , Autophagy , Breast Neoplasms/drug therapy , Cell Cycle Checkpoints , Cell Line, Tumor , Cell Proliferation , Female , Humans
4.
Chinese Journal of Endemiology ; (12): 871-876, 2021.
Article in Chinese | WPRIM | ID: wpr-909115

ABSTRACT

Objective:To screen differential metabolites and metabolic pathways in urine of adult patients with Kashin-Beck disease (KBD), so as to provide scientific basis for finding specific biomarkers and pathogenesis of KBD.Methods:In Yongshou County, the KBD area in Shaanxi Province, adult KBD patients were selected as the case group, and healthy people without clinical symptoms of KBD were selected as the control group in the same disease area. The subjects' fasting mid-morning urine was collected, and liquid chromatography-mass spectrometry (LC-MS) technology was used to detect small-molecule metabolites in the urine. Multivariate statistical analysis [partial least square discriminant analysis (PLS-DA)] and comparison with KEGG and human metabonomics database (HMDB) were used to identify and screen differential metabolites and metabolic pathways in KBD patients.Results:A total of 58 subjects were included, 39 cases in the case group, including 23 males and 16 females; the age was (61.2 ± 7.8) years old; the body mass index was (22.7 ± 6.5) kg/m 2. There were 19 cases in the control group, including 10 males and 9 females; the age was (50.0 ± 9.0) years old; the body mass index was (24.3 ± 5.5) kg/m 2. Three first-order differential metabolites (HT-2 toxin, T-2 tetraol and seleno-adenosine selenomethionine) were identified and screened, which were highly related to the pathogenesis of KBD, and all were down-regulated. There were 38 second-order differential metabolites, among them, 10 were up-regulated and 28 were down-regulated. Nine differential metabolic pathways were screened, mainly involving amino acid metabolism, lipid metabolism and energy metabolism. Conclusions:The urine metabolism profiles of adult KBD patients and healthy people are significantly different, mainly involving amino acid metabolism, lipid metabolism and energy metabolism. The first-order differential metabolites HT-2 toxin, T-2 tetraol and seleno-adenosine selenomethionine are highly correlated with the pathogenesis of KBD.

5.
Article in Chinese | WPRIM | ID: wpr-906758

ABSTRACT

@#Pancreatic cancer stroma plays a critical role in tumor progression, invasion, metastasis and resistance.Targeting tumor cell alone could not meet the demand for prolonging patients'' survival.Growing studies have laid emphasis on developing combined regimens between targeting pancreatic cancer stroma and chemotherapy, radiotherapy and immunotherapy.We are faced with some new opportunities in spite of the great challenges brought to the research and development of targeting drugs owing to the complicated stroma components, crosstalking signal pathways and abnormal angiogenesis of pancreatic cancer.In this article, recent advances in therapeutic strategies of targeting pancreatic cancer stroma are reviewed and analyzed from the aspects of extracellular matrix (ECM), cancer associated fibroblasts (CAFs) and vessels, in the hope of providing some novel ideas for targeting therapy against pancreatic cancer.

6.
Article in Chinese | WPRIM | ID: wpr-906114

ABSTRACT

Objective:To observe the clinical efficacy of modified Chushi Juanbitang combined with pedicle vertebrotomy on kyphosis of ankylosing spondylitis due to syndrome of dampness-heat obstruction. Method:The 90 cases were randomly divided into control group and observation group, 45 cases in each group. The patients in control group received pedicle vertebrotomy + <italic>Tripterygium</italic> glycosides, and the patients in observation group received pedicle vertebrotomy + modified Chushi Juanbitang. The treatment course was 6 months in both groups. Their bath ankylosing spondylitis disease activity index (BASDAI), bath ankylosing spondylitis measure index (BASMI), imaging index, traditional Chinese medicine syndromes, serum proinflammatory factor, anti-inflammatory factor, bone metabolism index [bone-specific alkaline phosphatase (BALP), tartrate resistant acid phosphatase isomer-5b (TRACP-5 b), bone morphogenetic protein-2 (BMP-2), osteocalcin (BGP)], ossification related proteins [bone morphogenetic protein-7 (BMP-7), dickkopf-related protein-1 (DKK-1), and tissue inhibitor matrix metalloproteinase-2 (TIMP-2), sclerostin(SOST)] were observed and detected. The clinical efficacy, recurrence rate and safety indexes were followed up for 12 months and compared. Result:The total effective rate was 97.73% (43/44) in the observation group, higher than 80.95% (34/42) in the control group (<italic>χ</italic><sup>2</sup>=5.172, <italic>P</italic><0.05). In the comparison with control group after treatment, the BASDAI, BASMI, imaging index, traditional Chinese medicine syndromes, proinflammatory factors, TRACP-5b, BMP-7 and TIMP-2 were lower in observation group (<italic>P</italic><0.05), and the anti-inflammatory factors, BALP, BMP-2, BGP, DKK-1 and SOST were higher in observation group (<italic>P</italic><0.05). During the follow-up for at least 12 months, the recurrence rate was 4.65% (2/43) in observation group, lower than 26.47% (9/34) in control group (<italic>χ</italic><sup>2</sup>=4.261, <italic>P</italic><0.05). There was no significant difference in the incidence of postoperative complications between the two groups. The incidence of adverse reactions was 2.27% (1/44) in observation group, lower than 38.64% (17/44) in control group (<italic>χ</italic><sup>2</sup>=5.763, <italic>P</italic><0.05). Conclusion:Modified Chushi Juanbitang combined with pedicle vertebrotomy is effective in the treatment of kyphosis of ankylosing spondylitis due to syndrome of dampness-heat obstruction.

7.
Journal of Experimental Hematology ; (6): 1935-1939, 2021.
Article in Chinese | WPRIM | ID: wpr-922227

ABSTRACT

OBJECTIVE@#To analyze the causes of positive irregular antibody screening test and incompatibility of cross matching in one patient with autoimmune hemolytic anemia complicated with neonatal hemolytic disease, and to accurately identify the type of antibodies in patients, and to select a reasonable strategy for blood transfusion.@*METHODS@#One children was enrolled, blood group positive and reverse typing, Rh typing, direct anti-human globulin test, free test, dispersal test and cross matching test were carried out by test tube method and microcolumn gel card; irregular antibodies were identified by the reaction of DTT treatment and untreated panel cells with patients' plasma.@*RESULTS@#The blood group of the patient was RhD positive B and irregular antibody screening positive, while the blood group of the mother was RhD positive O and irregular anti-screening negative, the result showed that the anti-LW detected in the plasma of the patient was autoantibody and ABO neonatal hemolytic disease (ABO-HDN) was present. Both O type RhD positive washing RBCs and B type RhD negative RBCs were transfused effectively.@*CONCLUSION@#Irregular antibodies in patients are anti-LW antibodies, and transfusion of homotype RhD negative suspended erythrocytes after the exclusion of ABO-HDN shows a better effect.


Subject(s)
Anemia, Hemolytic, Autoimmune , Autoantibodies , Blood Group Incompatibility , Blood Transfusion , Erythroblastosis, Fetal , Humans
8.
Article in Chinese | WPRIM | ID: wpr-864521

ABSTRACT

Objective:To retrieve and select the available evidence on preventing the fall on the home-based elderly people and summarize the best available evidence.Methods:The BMJ Best Practice, UpToData, Registered Nurses′ Association of Ontario (RANO), National Guideline Clearinghouse (NGC), National Institute for Health and Care Excellence (NICE), Joanna Briggs Institute (JBI), Cochrane Library, and complementally the Center for Disease Control and Prevention(CDC), PubMed、CNKI and SinoMed were searched to collect literatures including guideline, evidence summary, best practice information sheet, recommended practice, systematic review/Meta-analysis.Results:13 articles were selected, including 6 guidelines, 1 clinical decision, 1 evidence summary, 5 systematic reviews/Meta-analysis. Finally, 8 items of best evidence were summarized.Conclusions:Healthcare workers should appropriately consult the best evidence to prevent falling and falling injuries for the home-based elderly people, and finally improve their health-related quality of life.

9.
Article in Chinese | WPRIM | ID: wpr-871269

ABSTRACT

Objective:To study whether simvastatin could inhibit viral replication during human metapneumovirus (hMPV) infection.Methods:Human bronchial epithelial cells (16HBE) were infected with hMPV and then treated with or without simvastatin. Real-time quantitative PCR (qPCR) and Western blot were used to detect virus titers and the activation of autophagy and related pathways. BALB/c mice were infected with hMPV and then treated with simvastatin through intragastric administration. Pathological changes in lung tissues were observed. Changes in viral loads and the activation of autophagy and related pathways in proteins and RNA extracted from lung tissues were detected.Results:The in vitro experiment showed that the hMPV+ simvastatin group had decreased virus titer and enhanced autophagy than the hMPV group. The AKT/mTOR pathway in the hMPV+ simvastatin group was inhibited, which was verified by a further experiment using rapamycin, a specific inhibitor of AKT/mTOR pathway. The in vivo experiment showed that the virus titer in the hMPV+ simvastatin group was lower than that in the hMPV group, but there was no significant difference in the activation of autophagy. The AKT/mTOR pathway was down-regulated in the hMPV+ simvastatin group. HE staining revealed that obvious pathological changes were observed in the hMPV group, but the condition was improved after simvastatin intervention. Conclusions:Simvastatin can inhibit the replication of hMPV, which is associated with the activation of autophagy induced by AKT/mTOR pathway.

10.
Article in Chinese | WPRIM | ID: wpr-869267

ABSTRACT

Objective:To investigate the prevalence and risk factors of stroke in Xuzhou city.Methods:A total of 41 932 residents aged 18 years and above were selected using the multi-stage stratified cluster sampling method. A questionnaire survey was conducted to investigate the rate of stroke, and a physical examination was performed to investigate height, weight, blood pressure, etc. Univariate analyses of stroke were performed using the Chi square test and trend chi-square test. Logistic regression analysis was performed for multi-factor analysis.Results:A total of 39 854 participants (19 222 males, 10 323 from urban areas) from 41 932 eligible participants were included in the statistical analysis, and their average age was (52.1±16.8). In this study, 885 stroke patients (464 males, 302 from urban areas) were found among 39 854 participants. The prevalence of stroke was 2 220.61/100 000, which was separately 2 413.90/100 000 and 2 040.52/100 000 among males and females. The difference between males and females was significant (χ2=6.22, P=0.013). The prevalence of stroke in urban areas (2 925.51/100 000) was higher than in rural areas (1 974.20/100 000) (χ2 =31.45, P<0.001). The results of univariate analysis revealed that the risk factors for stroke were [ OR(95% CI)] smoking [1.82(1.53-2.08)], drinking [1.22(1.08-1.81)], sleep quality [1.42(1.13-1.96)], physical activities [1.44(1.11-2.14)], hypertension [3.53(2.44-6.02)], heart disease [1.23(1.11-1.75)], diabetes [1.42(1.31-2.05)], family history of hypertension [1.43(1.30-2.37)], family history of diabetes [1.22(1.10-1.65)], and family history of stroke [1.57(1.46-2.06)]. Logistic regression analysis showed that age [3.02(2.14-4.96)], smoking [1.59(1.12-3.85)], poor sleep quality [1.15(1.03-3.23)], lack of physical activity [1.22(1.08-3.38)], hypertension [4.53(3.07-7.36)], diabetes [1.08(1.02-3.23)], and family history of stroke [1.15(1.08-3.31)] were related to stroke. Conclusion:The epidemic of stroke in Xuzhou city was relatively high, and prevention and control measures of stroke should be taken according to the risk factors of stroke in the population distribution.

11.
Article in Chinese | WPRIM | ID: wpr-849740

ABSTRACT

Objective: To investigate the effect of simvastatin pretreatment on human metapneumovirus (hMPV) infection and the related mechanism. Methods: Human bronchial epithelial cells (16HBE) were used in vitro experiments and BALB/c mice were used in vivo experiments. They were randomly divided into control group, simvastatin group, hMPV group, hMPV+simvastatin group. After corresponding treatment, the fluorescence of hMPV was observed with microscopy; virus titer and the expressions of autophagy-related gene recombinant human autophagy-related protein 5 (ATG5), Beclin1, and microtubule-associated protein 1 light chain 3 (LC3) were detected by Fluorescence Quantitative Real-time PCR; Western blotting was performed to detect autophagy-related protein ATG5, Beclin1, LC3 and serine/threonine kinase/mammalian rapamycin target protein (AKT/mTOR) pathway proteins. Results: In vitro experiment, the direct and indirect fluorescence expressions of hMPV and the virus titer were lower in hMPV+simvastatin group (0.260 ± 0.018) than those in hMPV group (1.241 ± 0.030), while the mRNA expression levels of autophagy-related gene ATG5, Beclin1 and LC3 were higher in hMPV+simvastatin group [(7.31±0.15), (8.67±0.88) and (6.55±0.30), respectively] than those in control group [(1.00±0.06), (1.00±0.05) and (1.10±0.06), respectively]; the mRNA and protein levels of autophagy-genes were the highest in hMPV+simvastatin group and the differences were statistically significant (P<0.05). The AKT/mTOR pathway was inhibited in hMPV group and hMPV+simvastatin group. In vivo experiment, the virus titer of the lung tissue and of the lung tissue supernatant inoculated in Vero E6 were lower in hMPV+simvastatin group (0.75±0.26 and 0.42±0.17, respectively) than those in hMPV group (2.46±0.53 and 1.80±0.40, respectively). The mRNA and protein expression levels of autophagy-related genes ATG5, Beclin1 and LC3 were the highest in hMPV+simvastatin group (3.89±0.42, 3.13±0.26 and 3.56±0.22, respectively), higher than those in control group and hMPV group, the differences were statistically significant (P<0.05), and the AKT/mTOR pathway was inhibited in this group; HE staining of lung tissue showed an inflammatory response in the hMPV group, and pre-treatment with simvastatin could reduce the inflammation. Conclusion: Pretreatment with simvastatin may reduce human metapneumovirus infection, which is associated with autophagy and the AKT/mTOR pathway.

12.
Article in Chinese | WPRIM | ID: wpr-878687

ABSTRACT

Segmental zoster paresis(SZP)is a rare complication in herpes zoster infection,with its symptoms often neglected due to the co-existence of pain.Here we reported a case of SZP.Also,we analyzed 42 Chinese SZP cases in literature,which revealed that the male to female ratio of SZP patients was 13∶8,and the median age of disease onset was 65 years.The most commonly affected region is upper limb.The diagnosis depends mainly on typical medical history and clinical symptoms.Although there is no definite therapy for SZP,the antiviral therapy is the most commonly used treatment,which achieved complete recovery in 78.6% of the patients and partial recovery in 14.3% of the patients.


Subject(s)
Aged , Female , Herpes Zoster/diagnosis , Humans , Male , Paresis/etiology , Upper Extremity/physiopathology
13.
Chinese Journal of Burns ; (6): 110-116, 2020.
Article in Chinese | WPRIM | ID: wpr-799484

ABSTRACT

Objective@#To observe the effect of ulinastatin combined with glutamine on early hemodynamics in patients with severe burns.@*Methods@#Thirty-two patients with severe burns who met the inclusion criteria and hospitalized in the Affiliated Huaihai Hospital of Xuzhou Medical University from January 2016 to December 2018 were selected for conducting a prospective randomized controlled trial. According to the random number table, the patients were divided into conventional treatment group (4 males and 4 females), ulinastatin group (5 males and 3 females), glutamine group (5 males and 3 females), and ulinastatin+ glutamine group (4 males and 4 females), with ages of (36±8), (34±8), (35±9), and (38±13) years in turn. From post injury day 2, patients in the 4 groups were given nutritional support of equal nitrogen and equal calories, of which protein was 2.0 g/kg daily. In addition, patients in the ulinastatin group received intravenous injection of 100 kU ulinastatin every 8 hours for 7 consecutive days; 0.3 g/kg of protein given to patients in the glutamine group was provided by alanine glutamine for 7 consecutive days; patients in the ulinastatin+ glutamine group received corresponding treatments of both ulinastatin group and glutamine group. With the help of pulse contour cardiac output (PiCCO) monitoring technology, the cardiac index, stroke volume index (SVI), global end-diastolic volume index (GEDI), systemic vascular resistance index (SVRI), extravascular lung water index (EVLWI), pulmonary vascular permeability index (PVPI) of patients in each group were measured on treatment day (TD) 1, 3, and 7. Data were processed with Fisher′s exact probability method, one-way analysis of variance, analysis of variance for repeated measurement, and Bonferroni method.@*Results@#The cardiac index was low and the SVI value was lower than the normal value on TD 1 in patients of the 4 groups, without statistically significant differences between any two groups (P>0.05), and then they were all gradually increased. On TD 3 and 7, compared with those of the conventional treatment group, the cardiac index and SVI of patients in the other three groups were all increased, and the cardiac index and SVI of patients in the ulinastatin+ glutamine group were significantly increased (P<0.05 or P<0.01). On TD 1, the GEDI of patients in the conventional treatment group, ulinastatin group, glutamine group, and ulinastatin+ glutamine group were at normal low levels, which were (659±58), (661±79), (659±88), and (653±71) mL/m2 respectively, without statistically significant differences between any two groups (P>0.05), and then they all gradually increased. On TD 3 and 7, compared with (684±82) and (742±46) mL/m2 of the conventional treatment group, the GEDI of patients in the ulinastatin group, glutamine group, and ulinastatin+ glutamine group were all elevated, which were (732±53) and (777±33), (725±58) and (783±49), (813±65) and (849±27) mL/m2 respectively, and the GEDI of patients in the ulinastatin+ glutamine group was significantly increased (P<0.05). The SVRI of patients in the four groups were all at high levels on TD 1, without statistically significant differences between any two groups (P>0.05), and then they all gradually decreased. On TD 3 and 7, compared with those of the conventional treatment group, the SVRI of patients in the other three groups were all increased, and the SVRI in the ulinastatin+ glutamine group was significantly increased (P<0.05). On TD 1, the EVLWI of patients in the conventional treatment group, ulinastatin group, glutamine group, and ulinastatin+ glutamine group were all in the normal range, which were (6.6±0.6), (6.3±0.4), (6.5±0.4), and (6.6±0.6) mL/kg respectively, without statistically significant differences between any two groups (P>0.05), and then they all showed the increasing trend. On TD 3 and 7, compared with (7.1±0.9) and (7.9±0.5) mL/kg of the conventional treatment group, the EVLWI of patients in the ulinastatin group, glutamine group, and ulinastatin+ glutamine group were all decreased, which were (6.2±0.6) and (7.1±0.4), (6.3±1.0) and (7.2±0.9), (5.8±0.7) and (6.7±0.6) mL/kg respectively, and the EVLWI of patients in the ulinastatin+ glutamine group was significantly decreased (P<0.05). On TD 1, the PVPI of patients in the four groups were all in the normal range, without statistically significant differences between any two groups (P>0.05), and then they all gradually decreased. On TD 3 and 7, compared with those of the conventional treatment group, the PVPI of patients in the other three groups were all decreased, and the PVPI in the ulinastatin+ glutamine group was significantly decreased (P<0.05).@*Conclusions@#Ulinastatin combined with glutamine can increase the cardiac index, SVI, GEDI, and SVRI and reduce the EVLWI and PVPI in treating patients with severe burns, thereby increasing early cardiac output after injury, promoting tissue and organ perfusion, and reducing pulmonary edema, resulting in significant improvement in early hemodynamics of patients with severe burns.

14.
Journal of Chinese Physician ; (12): 951-954, 2019.
Article in Chinese | WPRIM | ID: wpr-754247

ABSTRACT

Cervical cancer is an important global public health problem,and its incidence tends to be younger in China.Compared with stage ⅡB cervical cancer,concurrent chemoradiotherapy has obvious advantages in reducing local recurrence rate,improving progression free survival rate,overall survival rate,and improving prognosis.Concurrent chemoradiotherapy and surgical treatment can effectively reduce local lesions and increase the opportunity for surgical resection.Synchronous radiotherapy and chemotherapy do not increase the difficulty of operation and postoperative complications.At the same time,all kinds of short-term and long-term complications caused by radical radiotherapy are avoided.The new adjuvant chemotherapy will strive for radical surgery,using radical surgery to eliminate the focus of radiotherapy and chemotherapy,improve the patient's survival time and improve the patient's quality of life.Preoperative radiotherapy can also maintain the physiological function of the vagina and avoid various complications caused by the amount of radical radiotherapy.

15.
Practical Oncology Journal ; (6): 92-96, 2019.
Article in Chinese | WPRIM | ID: wpr-752820

ABSTRACT

Local and/or regional recurrence is a common cause of the failure of primary radical radiotherapy for nasopharyn-geal carcinoma,and it is also a clinically existing treatment problem. Re-radiation therapy is one of main effective means of treatment for recurrent nasopharyngeal carcinoma. The article summarizes the recent domestic and abroad literatures on radiotherapy in vitro(in-cluding conventional two-dimensional,three-dimensional conformal,intensity-modulated radiotherapy),stereotactic radiotherapy, brachytherapy,proton beam and heavy ion therapy for recurrent nasopharyngeal carcinoma,and summarized the advantages and disad-vantages of different radiotherapies as well as their efficacies.

16.
Article in Chinese | WPRIM | ID: wpr-800876

ABSTRACT

With the progress of the hierarchical medical system, the top-level design for building medical alliances is exhibiting a clearer picture. The Chinese government sets about trying to build a grid-based medical system layout, covering from national, provincial, municipal, county, township, all the way to village level. Based on the analysis of regional grid-based medical systems in the United States, Switzerland, Singapore and Canada, the authors reviewed the development of grid-based health service system in China, and found out key roadblocks for grid-based medical alliances. These problems include the organizational structure, service content, management mechanism, payment system and other aspects, and corresponding countermeasures and suggestions were proposed in the end.

17.
Article in Chinese | WPRIM | ID: wpr-796357

ABSTRACT

Objective@#To screen and verify the differential expression profiles of long non-coding RNAs(LncRNAs) in peripheral blood of patients with type 2 diabetes mellitus(T2DM), to identify the potential molecular specific markers of early T2DM.@*Methods@#The blood samples of 4 type 2 diabetic patients and 4 normal control subjects were collected for microarray analysis. Then six candidate markers of LncRNAs screened from the differential expression profile were tested by qRT-PCR among the subjects (80 cases in the T2DM group and 50 cases in the control group). The possibility of these LncRNAs as molecular diagnostic markers was analyzed, and finally two of them were carried out by receiver operating characteristic (ROC) analysis.@*Results@#Compared with control subjects, there were differentially expressed 133 LncRNAs in type 2 diabetic patients, among which 5 were up-regulated with the maximum up-regulated fold 3.29 and 128 were down-regulated with the maximum down-regulated fold 8.99. Six down-regulated LncRNAs were selected for validation and revealed a similar result to that of microarray.The expressions of two LncRNAs(NONHSAT160746 and NONHSAT140069) in peripheral blood of diabetic patients were significantly lower than those of control subjects (P<0.01). The areas under the ROC curve of the two LncRNAs were 0.734 and 0.703, respectively(P<0.01).@*Conclusion@#LncRNAs NONHSAT160746 and LncRNAs NONHSAT140069 are the potential molecular specific markers for the diagnosis of diabetes mellitus.

18.
Article in Chinese | WPRIM | ID: wpr-791727

ABSTRACT

Objective To screen and verify the differential expression profiles of long non-coding RNAs (LncRNAs) in peripheral blood of patients with type 2 diabetes mellitus(T2DM), to identify the potential molecular specific markers of early T2DM. Methods The vein blood samples of 4 type 2 diabetic patients and 4 normal control subjects were collected for microarray analysis. Then six candidate markers of LncRNAs screened from the differential expression profile were tested by qRT-PCR among the subjects ( 80 cases in the T2DM group and 50 cases in the control group). The possibility of these LncRNAs as molecular diagnostic markers was analyzed, and finally two of them were carried out by receiver operating characteristic (ROC) analysis. Results Compared with control subjects, there were differentially expressed 133 LncRNAs in type 2 diabetic patients, among which 5 were up-regulated with the maximum up-regulated fold 3.29 and 128 were down-regulated with the maximum down-regulated fold 8.99. Six down-regulated LncRNAs were selected for validation and revealed a similar result to that of microarray.The expressions of two LncRNAs(NONHSAT160746 and NONHSAT140069) in peripheral blood of diabetic patients were significantly lower than those of control subjects (P<0.01). The areas under the ROC curve of the two LncRNAs were 0.734 and 0.703, respectively(P<0.01). Conclusion LncRNAs NONHSAT160746 and LncRNAs NONHSAT140069 are the potential molecular specific markers for the diagnosis of diabetes mellitus.

19.
Article in Chinese | WPRIM | ID: wpr-852077

ABSTRACT

Objective: By investigating the material composition, anti-oxidant and hypoglycemic activity of acetonitrile-water extracts of Origanum vulgare leaf (OL) in different harvest time, to acquire the scientific data for the utilization of OL. Methods: OL was extracted with acetonitrile and water (1: 1). The contents of total phenols and total flavonoid were measured by Foline-Phenol reagent method and AlCl3 colorimetry. The main compositional analysis was performed using LC-QTOF-MS technology. Meanwhile, the anti-oxidation of OL extracts in different harvest time was evaluated by total anti-oxidant capacity assay kit with ABTS method and ferric reducing anti-oxidant potential assay (FRAP). The methods of 2-NBDG glucose uptake and α-glycosides inhibition were applied to evaluate hypoglycemic activity of OL extracts. Results: The six main components in OL extract were identified to be origanoside I, luteolin 7-O-glucuronide, apigenin 7-O-glucuronide, rosmarinic acid, lithospermic acid and origanoside I derivative. The contents of total phenols and the total flavonoids were the highest in OL extracts harvested in July and September, respectively. There was good in vitro anti-oxidant and hypoglycemic activity for OL extracts harvested in different times. Among them, the best anti-oxidant activity was observed in OL extracts harvested in July, while the best hypoglycemic activity was observed in OL extracts harvested in October. Conclusion: OL has potential anti-oxidant and hypoglycemic activity, which is affected by the harvest time.

20.
Article in Chinese | WPRIM | ID: wpr-752192

ABSTRACT

Objective: To analyze the chemical constituents of Morchella esculenta, and to determine the main activeingredient of it, so as to better control the quality of Morchella esculenta and promote the development and utilization of Morchella. Methods: The compounds were extracted and percolated by ethanol. The samples were separated using silicagel and identified by13C-NMR data. HPLC was used to assay the contents of 4-Hydroxybenzyl alcohol. Results: A totalof 5 compounds were isolated and their structures were identified as 11, 15, 19-trimethyl-5, 9-eicosadienoic acid, cis-13-Docosenoic acid&Erucic acid, 4-Hydroxybenzyl alcohol, Ergosta-5, 7, 22-triene-3β-ol and Mannitol. Conclusion: 4-Hydroxybenzyl alcohol was first isolated from the Morchella esculenta fruiting body, and HPLC method for thedetermination of 4-Hydroxybenzyl alcohol was established. The content of 4-Hydroxybenzyl alcohol were no less than0.40%. The determination method can be used for quality control of Morchella esculenta.

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