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1.
China Pharmacy ; (12): 90-95, 2022.
Article in Chinese | WPRIM | ID: wpr-907019

ABSTRACT

OBJECTIVE To eval uate the effectiveness ,safety and economy of deferasir ox for the treatment of iron overload in thalassemia with rapid health technology assessment ,and to provide evidence-based basis for rational clinical use. METHODS Retrieved from Chinese and English database/website as PubMed ,Embase,Cochrane Library ,NHS EED ,CADTH,CNKI and Wanfang database ,health technology assessment (HTA),systematic evaluation/meta-analysis and pharmacological studies about deferasirox versus deferoxamine/deferiprone for the treatment of iron overload in thalassemia were collected from the inception to June 2021. Based on literature screening and data extraction ,the quality of literature about HTA reports ,systematic evaluation/ Meta-analysis and pharmacoeconomic research were evaluated with HTA checklist ,A Measurement Tool to As sess Systematic Reviews,standard scale of economic evaluation report. The effectiveness and safety results were described quantitatively ,and the economic evaluation results were described qualitatively. RESULTS One HTA report ,five systematic evaluation/meta-analysis and five pharmacoeconomic studies were selected from 1 569 literature. Included HTA reports , systematic evaluation/meta-analysis,pharmacoeconomic studies were high in quality. Most studies reported that 30 mg/(kg·d) deferasirox was E-mail:aydgs@126.com better than deferoxamine in reducing the levels of s erum ferritin and liver iron overload ;ADR induced by deferasirox were mainly gastrointestinal irritation symptoms ,skin itching ,joint pain,transaminase elevation ,etc.,which generally did not affect subsequent treatment. There was no statistical significance in severe ADR between deferoxamine group and deferasirox group [RR =0.96,95%CI(0.85,1.08),P=0.52]. Compared with deferoxamine,deferasirox had higher cost-effectiveness ;but deferasirox was less likely to be cost-effective than deferiprone. CONCLUSIONS Deferasirox has good effectiveness and safety for iron overload in thalassemia ,and has good economic advantages in Britain and Iran ,compared with deferoxamine.

2.
Article in Chinese | WPRIM | ID: wpr-907804

ABSTRACT

Objective:To investigate the safety and efficacy of da Vinci surgical system in surgical treatment of primary hyperparathyroidism.Methods:The clinical data of 19 patients with primary hyperparathyroidism who received the Da Vinci robot surgical system (the da Vinci surgery group) from Feb. 2016 to May. 2020 and 23 patients undergoing open parathyroid surgery (the open surgery group) were retrospectively analyzed and compared. The operation time, intraoperative blood loss, postoperative drainage volume, hospital stay, postoperative serum calcium and phosphorus, parathyroid hormone, postoperative pain visual analog score, and surgical complications rate and beauty effect of the two groups were statistically compared. The postoperative clinical symptoms and recurrence were followed up in a duration 3 to 84 months.Results:All patients completed the operation successfully, there was no conversion to open operation in the da Vinci surgery group. The operation time (65.5±9.9 vs 54.3±8.3) min ( t=6.231, P=0.015) and postoperative drainage volume (109.7±50.6 vs 97.2±45.2) ml ( t=3.132, P=0.016) in the da Vinci surgery group were more than those in the open surgery group, while the cosmetic effect (9.1±0.6 vs 8.3±0.7) ( t=3.628, P=0.031) was better in the da Vinci surgery group than in the open surgery group. There was no significant difference in intraoperative blood loss (44.3±19.4 vs 39.1±15.4) ml, hospital stay (7.4±1.4 vs 7.9±2.8) days, incidence of complications (15.8% vs 8.7%) , visual analogue scale of postoperative pain (6.9±0.6 vs 6.4±0.8) , clinical symptom relief during the follow-up (100.0% vs 100.0%) , postoperative serum calcium (2.48±0.30 vs 2.43±0.26) mmol/L, serum phosphorus (0.75±0.07 vs 1.37±0.31) mmol/L or parathyroid hormone (36.5±4.7 vs 40.4±5.3) ng/L between the da Vinci surgery group and the open surgery group ( P>0.05) . Conclusion:Leonardo da Vinci robot-assisted surgical treatment for primary hyperparathyroidism with strict surgical indications is similar to traditional open surgery in safety and efficacy, while its cosmetic effect is better.

3.
Article in Chinese | WPRIM | ID: wpr-907427

ABSTRACT

The thioredoxin system is composed of thioredoxin (Trx), thioredoxin reductase (TR) and reduced nicotinamide adenine dinucleotide phosphate. Trx is an important antioxidant molecule that can resist cell death caused by various stresses and plays a prominent role in redox reactions. TR is a protein containing selenium (selenocysteine), mainly in three forms, i.e. TR1, TR2 and TR3. TR1 mainly distributed in the cytoplasm, TR2 mainly distributed in the mitochondria, and TR3 mainly distributed in the testes. TR can regulate cell growth and apoptosis. After the cell becomes cancerous, the expression of TR increases to promote cell growth and metastasis. Trx system is closely related to neurodegenerative diseases, parasitic infections, acquired immunodeficiency syndrome, rheumatoid arthritis, hypertension, myocarditis and so on. The Trx system can remove the reactive oxygen species (ROS) in the body, keep the inside and outside of the cell in a balanced state, and it interacts with the thioredoxin interacting protein (TXNIP), which plays an important role in the regulation of glucose metabolism and tumor treatment. The Trx system is an important target for drug treatment of many diseases. In this paper, the research progress of the thioredoxin system was reviewed.

4.
Article in Chinese | WPRIM | ID: wpr-906522

ABSTRACT

Objective:To investigate the intervention of <italic>Hedyotis diffusa</italic> (HDW) on colitis associated cancer (CAC) model mice and explore its mechanism. Method:The CAC mouse model was established by synergistic action of azoxymethane (AOM) and dextran sulfate sodium (DSS). The intervention of HDW on CAC mice was evaluated by disease activity index (DAI), colonic tissue morphology, pathological injury score and tumorigenesis rate. Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) and multivariate statistical analysis were used to analyze the metabonomics of mice serum and to explore the mechanism of HDW intervention on CAC. Result:HDW could significantly improve the general condition of CAC mice, decrease DAI, colon gross morphological score, histopathological score and tumorigenesis rate. Compared with the normal group, 38 kinds of differential metabolites were screened in the model group, including 11 potential biomarkers, involving 11 main metabolic pathways. HDW could significantly regulate 9 kinds of differential metabolites [niacinamide, uridine, 4-pyridoxic acid, LysoPC (18∶0), LysoPE (0∶0/20∶0), myo-inositol, purine, sphinganine 1-phosphate and tetradecanedioic acid] in the model group, including 2 kinds of potential biomarkers (myo-inositol and niacinamide), and HDW could regulate nicotinate and nicotinamide metabolism and inositol phosphate metabolism. Conclusion:HDW has a therapeutic effect on CAC, which may be achieved by regulation of energy metabolism and glucose metabolism.

5.
Article in Chinese | WPRIM | ID: wpr-905830

ABSTRACT

Objective:This studu aims to investigate the effect of aqueous extract of modified Xiao Xianxiongtang on the epithelial mesenchymal transition(EMT) and the change of its invasion and migration ability of human gastric cancer MGC-803 cells mediated by transforming growth factor-<italic>β</italic><sub>1</sub>(TGF-<italic>β</italic><sub>1</sub>),and to explore the mechanism of regulating Wnt5a/Ca<sup>2+</sup>/ activated T-cell nuclear factor(NFAT) signaling pathway to inhibit EMT and invasion and metastasis of MGC-803 cells. Method:TGF-<italic>β</italic><sub>1</sub>(10 μg·L<sup>-1</sup>)was used to induce EMT and the invasion and metastasis model of human gastric cancer MGC-803 cells. Transwell chamber experiment, scratchhealing experiment, Western blot and immunofluorescence assay were used to detect cell invasion and migration ability, expression of EMT marker protein and key protein expression of Wnt5a/Ca<sup>2+</sup>/NFAT signaling pathway, and intracellular Ca<sup>2+</sup> concentration. Result:Compared with the blank group, TGF-<italic>β</italic><sub>1</sub> could significantly enhance the invasion and migration ability of MGC-803 cells(<italic>P</italic><0.01), down-regulate the level of E-cadherin(<italic>P</italic><0.01), up-regulate protein expressions of N-cadherin, Snail and Vimentin(<italic>P</italic><0.01), and induce cell Wnt5a, calcineurin (CaN), total protein of activated T-cell nuclear factor 1(NFAT1), up-regulation of phosphorylated proteins p-NFAT1 and NFAT1 nucleoprotein and intracellular accumulation of Ca<sup>2+</sup>(<italic>P</italic><0.01). Compared with the TGF-<italic>β</italic><sub>1</sub> group, modified Xiao Xianxiongtang (10, 20, 40 mg·L<sup>-1</sup>) could significantly inhibit this phenomenon,and 40 mg·L<sup>-1</sup> had the best effect(<italic>P</italic><0.05,<italic>P</italic><0.01).The specific inhibitors of Wnt5a/Ca<sup>2+</sup>/NFAT signaling pathway (<italic>R</italic>)-(+)-Bay-K-8644 and modified Xiao Xianxiongtang (40 mg·L<sup>-1</sup>) could significantly inhibit theinvasion and migration of MGC-803 cells mediated by TGF-<italic>β</italic><sub>1</sub>, up-regulate the level of E-cadherin, and down-regulate expressions of N-cadherin, Snail, Vimentin, Wnt5a, CaN and NFAT1 proteins and reduce the intracellular accumulation of Ca<sup>2+</sup>(<italic>P</italic><0.05,<italic>P</italic><0.01).Moreover, (<italic>R</italic>)-(+)-Bay-K-8644 combined with modified Xiao Xianxiongtang (40 mg·L<sup>-1</sup>) had stronger inhibitory effect(<italic>P</italic><0.05,<italic>P</italic><0.01). Conclusion:These results suggest that modified Xiao Xianxiongtang can inhibit the EMT mediated by TGF-<italic>β</italic><sub>1</sub> via Wnt5a/Ca<sup>2+</sup>/NFAT signaling pathway,thereby reducing the invasion and migration ability of MGC-803 cells.

6.
Chinese Medical Journal ; (24): 2025-2036, 2021.
Article in English | WPRIM | ID: wpr-921118

ABSTRACT

Chronic obstructive pulmonary disease (COPD) is a heterogeneous disease characteristic of small airway inflammation, obstruction, and emphysema. It is well known that spirometry alone cannot differentiate each separate component. Computed tomography (CT) is widely used to determine the extent of emphysema and small airway involvement in COPD. Compared with the pulmonary function test, small airway CT phenotypes can accurately reflect disease severity in patients with COPD, which is conducive to improving the prognosis of this disease. CT measurement of central airway morphology has been applied in clinical, epidemiologic, and genetic investigations as an inference of the presence and severity of small airway disease. This review will focus on presenting the current knowledge and methodologies in chest CT that aid in identifying discrete COPD phenotypes.


Subject(s)
Airway Obstruction , Humans , Phenotype , Pulmonary Disease, Chronic Obstructive/diagnostic imaging , Pulmonary Emphysema/diagnostic imaging , Tomography, X-Ray Computed
7.
Chinese Acupuncture & Moxibustion ; (12): 1140-1144, 2021.
Article in Chinese | WPRIM | ID: wpr-921023

ABSTRACT

The paper summarizes the ideas and characteristics of acupoint selection in treatment of mental disorder with acupuncture and moxibustion by professor


Subject(s)
Acupuncture Points , Acupuncture Therapy , Humans , Mental Disorders/therapy , Meridians , Moxibustion
8.
Journal of Gastric Cancer ; : 439-456, 2021.
Article in English | WPRIM | ID: wpr-914973

ABSTRACT

Purpose@#Gastric cancer (GC) has high morbidity and mortality and is a serious threat to public health. The flavonoid compound vitexin is known to exhibit anti-tumor activity. In this study, we explored the therapeutic potential of vitexin in GC and its underlying mechanism. @*Materials and Methods@#The viability, migration, and invasion of GC cells were determined using MTT, scratch wound healing, and transwell assays, respectively. Target molecule expression was determined by western blotting. Tumor growth and liver metastasis were evaluated in vivo using nude mice. Protein expression in the tumor tissues was examined by immunohistochemistry. @*Results@#Vitexin inhibited GC cell viability, migration, invasion, and epithelial-mesenchymal transition (EMT) in a dose-dependent manner. Vitexin treatment led to the inactivation of phosphatidylinositol-3-kinase (PI3K)/AKT/hypoxia-inducible factor-1α (HIF-1α) pathway by repressing HMGB1 expression. Vitexin-mediated inhibition in proliferation, migration, invasion and EMT of GC cells were counteracted by hyper-activation of PI3K/AKT/HIF-1α pathway or HMGB1 overexpression. Finally, vitexin inhibited the xenograft tumor growth and liver metastasis in vivo by suppressing HMGB1 expression. @*Conclusions@#Vitexin inhibited the malignant progression of GC in vitro and in vivo by suppressing HMGB1-mediated activation of PI3K/Akt/HIF-1α signaling pathway. Thus, vitexin may serve as a promising therapeutic agent for the treatment of GC.

9.
Article in Chinese | WPRIM | ID: wpr-885345

ABSTRACT

The construction of featured specialties is the current development strategy of community health service institutions to improve the service scope and to meet the health needs of residents. The rehabilitation medicine has undergone 12 years of development and become a relatively mature featured specialty in Fenglin Community Health Service Center. Based on the Fenglin′s experience, this article discusses the development status and restriction bottlenecks of general practice, and the development status and trend of rehabilitation medicine in the community; and also explores the integrated development model of community-featured specialty with general practice.

10.
Article in Chinese | WPRIM | ID: wpr-885344

ABSTRACT

Community health institutions have entered a new development stage of featured specialty construction. After 12 years of development, rehabilitation medicine now is the featured specialty of Fenglin Community Health Service Center. This article presents the train of thought and key points of specialty construction in primary care institutions based on the Fenglin′s experience. The positioning of featured specialty should be based on the community. The construction process should include 7 elements, namely, the standard operation procedure(SOP)of service system construction, the detailed publicity and implementation of the collaboration of specialists, prevention and control knowledge promotion for general practitioners, prevention and control knowledge education for community residents, service list, clinical efficacy evaluation, and clinical database. In the later iterations, the head of the department should always focus on the service system construction SOP and clinical database construction, and the rest parts can be assigned to the relevant team members.

11.
Article in Chinese | WPRIM | ID: wpr-885343

ABSTRACT

Rehabilitation medicine is one of the most important specialties in community health institutions. This article introduces the 12 year′s development of rehabilitation medicine in Fenglin Community Health Service Center, focusing on the talent allocation, service capabilities, resource expansion, basic facilities, personnel recruiting, department operating, service scope, and its achievements and influence, to provide reference for planning and construction of featured specialty in community health service centers.

12.
Article in Chinese | WPRIM | ID: wpr-882703

ABSTRACT

Objective:To investigate the safety and surgical treatment effect of da Vinci robot in male thyroid surgery.Methods:Clinical data of 276 male patients undergoing robotic thyroid surgery with bilateral axillo-breast approach (BABA) admitted to our hospital from Feb. 2014 to Jan. 2020 were retrospectively analyzed. They were compared with 372 male patients (open group) who had open surgery by the same team during the same time. Surgical time, the amount of fluid selitransteise after surgery, the length of hospitalization after surgery, the number of metastatic lymph node (for malignant tumor) , cosmetic effect satisfaction, injury of recurrent laryngeal nerve and decrease of parathyroid function and other related surgical complications were analyzed. SPSS 20.0 software was employed to carry out statistical analysis, the measurement data were compared with t-test, and the counting data were analyzed by χ2 test. Results:All operations were successfully completed and on one in the robot group was converted into open surgery. Compared with the open group, surgical time of the robot group (benign: 96.78±9.67min, malignant: 143.93±15.73min) was significantly longer than that of the open group (benign: 70.40±12.49min, malignant: 112.26±15.72min) ( P<0.05) , but the postoperative beauty effect of the robot group (benign: 9.62±0.33 points, malignant: 9.59±0.31 min) was better than that of the open group (benign: 5.33±0.37 points, malignant: 5.87±1.65 points) ( P<0.05) . In patients with pathological malignancy, the average age of the robot group (40.89±11.45 years) was lower than that of the open group (44.84±11.88 years) (P<0.05) . There was no significant difference in the amount of fluid induced after surgery, the length of hospital stay after surgery or the number of cases with lymph node metastasis in the two groups ( P>0.05) . There were 2 cases in the robot group and 5 cases in the open surgery group had recurrent metastasis in the side of the neck after surgery. None of the patients had permanent larynx reflux nerve damage or permanent parathyroid dysfunction. The average follow-up time was (25.36±16.13) months, ranging from 1 to 72 months. Conclusion:Compared with traditional open surgery, da Vinci robot surgery system is safe and feasible in male thyroid surgery, with better beauty effect, and provides new options for male thyroid surgery patients with neck beauty needs.

13.
China Pharmacy ; (12): 1272-1276, 2021.
Article in Chinese | WPRIM | ID: wpr-876898

ABSTRACT

OBJECTIVE:To provide referenc e for the construction and software development of knowledge base for rational use of TCM decoction pieces. METHODS :By reviewing the literatures on rational drug use software and TCM decoction pieces in recent years ,the clinical characteristics of rational drug use of TCM decoction pieces as well as the characteristics and shortcomings of existing rational drug use software in the detection of rational drug use of TCM decoction pieces were analyzed , and the core contents and difficulties in the construction of knowledge base of rational drug use software of TCM decoction pieces were summarized. RESULTS & CONCLUSIONS :Clinical application of TCM decoction pieces was mainly based on “syndrome differentiation”,which reflected the unity of dialectics ,treatment,prescription selection and medication. Therefore ,the consideration of the rationality of clinical use of TCM decoction pieces could not blindly imitate the evaluation method of chemical medicine. Current rational drug use software was not based on the theoretical system of traditional Chinese medicine ,and it was not comprehensive and mature in the aspect of rational drug use review of TCM decoction pieces ,and lacks the knowledge base that could meet the requirements of rational use of TCM decoction pieces. Therefore ,it is necessary to construct a set of knowledge base which can meet the evaluation requirements of “consistency of principle ,method and prescription use ”of TCM decoction pieces under the guidance of TCM theoretical system. Its contents include that patient information collection ,construction of knowledge base related to diseases and syndromes ,selection of processed products of TCM dec oction pieces ,addition andsubtraction of clinical symptoms ,selection taboo of varieties of TCM decoction pieces , compatibility taboo , combined application of Chinese patent medicine or chemical medicine , dosage of TCM decoction pieces , total dosage and tastquantity of each prescription , special de coction drugs , medication methods and administration frequency ,etc. There are still some difficulties in the development of rational drug use software of TCM decoction pieces ,such as the construction of disease and syndrome related knowledge base and the difficulty in judging the rationality of clinical symptom addition and subtraction.

14.
Article in Chinese | WPRIM | ID: wpr-888624

ABSTRACT

OBJECTIVE@#According to the digital image features of corneal opacity, a multi classification model of support vector machine (SVM) was established to explore the objective quantification method of corneal opacity.@*METHODS@#The cornea digital images of dead pigs were collected, part of the color features and texture features were extracted according to the previous experience, and the SVM multi classification model was established. The test results of the model were evaluated by precision, sensitivity and @*RESULTS@#In the classification of corneal opacity, the highest @*CONCLUSIONS@#The SVM multi classification model can classify the degree of corneal opacity.


Subject(s)
Animals , Corneal Opacity , Support Vector Machine , Swine
15.
Chinese Journal of Cardiology ; (12): 118-122, 2020.
Article in Chinese | WPRIM | ID: wpr-799404

ABSTRACT

Objective@#To compare the long-term outcomes in ST-elevation myocardial infarction (STEMI) patients who underwent early or late delayed percutaneous coronary intervention (PCI) using drug-eluting stents (DES).@*Methods@#This study was a retrospective, observational and single-center study. Consecutive STEMI patients (n=977), who admitted to Fuwai Hospital in 2013 and underwent successful selective PCI using drug-eluting stents (DES) within 3 to 35 days after symptom onset were enrolled and divided into the early delayed PCI (3-14 d) group (n=495) and the late delayed PCI (15-35 d) group (n=482). General clinical data of the patients and related data of coronary angiography and interventional therapy were collected, and the endpoint events were followed up. The primary endpoint was 2-year major adverse cardiac and cerebrovascular events (MACCE) including cardiac death, recurrent myocardial infarction, definite or probable stent thrombosis and ischemic stroke. The secondary endpoint was 2-year ischemia-driven target vessel revascularization. The incidence of endpoint events of the two groups was compared, and it was compared again after the primary baseline characteristics such as age and gender were matched by the propensity scoring method at a 1∶1 ratio.@*Results@#A total of 910 (93.1%) patients who underwent delayed PCI were transferred from other hospitals, and 292 (29.9%) patients received thrombolysis before PCI. The time interval before PCI was 14 (10, 20) days. The incidence of 2-year MACCE (3.0%(15/495) vs. 2.3%(11/482), P=0.468) and ischemia-driven target vessel revascularization (3.8%(19/495) vs. 5.0%(24/482), P=0.385) were similar between the two groups. The incidence of 2-year MACCE (3.3%(15/453 vs. 2.4%(11/453), P=0.426) and ischemia-driven target vessel revascularization (4.2% (19/453) vs. 4.9%(22/453), P=0.632) were also similar between the two groups after matching propensity score.@*Conclusion@#The long-term clinical outcomes after early delayed PCI using DES is statistically equivalent to those of late delayed PCI using DES for STEMI patients who missed the time window for emergency PCI.

16.
Article in Chinese | WPRIM | ID: wpr-865146

ABSTRACT

Proximal gastrectomy, the surgical treatment of gastric upper adenocarcinoma or early esophagogastric junction adenocarcinoma, has received more and more attention. However, pathophysiological changes after proximal gastrectomy lead to a high risk of severe gastroesophageal reflux or reflux esophagitis, such as loss of mechanical anti-regurgitation barrier and receptive relaxation, decrease of peristalsis of remnant, incoordinate contraction, pylorus spasm, which limit its widespread use. Dozens of digestive tract reconstruction methods with different anti-reflux design have been reported in recent years. These strategies could be divided into 3 categories: buffer zone method, reconstruction of mechanical anti-regurgitation barrier, speeding up gastric emptying. The authors analyze the pathophysiological changes related with gastroesophageal reflux after proximal gastrectomy from the perspective of anatomy and phy-siology, summarize the advantages and disadvantages of anti-regurgitation methods, and look forward to the development trends in the future.

17.
International Journal of Surgery ; (12): 739-743, 2020.
Article in Chinese | WPRIM | ID: wpr-863414

ABSTRACT

Objective:To evaluate the feasibility and safety of robotic thyroidectomy for treatment of thyroid benign tumor with diameter larger than 5cm.Methods:The clinical data of 36 patients (in the robot group) who received Da Vinci robot thyroid benign tumor surgery in the thyroid breast Surgery Department of the 960th Hospital of the PLA (Former Jinan Military General Hospital of PLA) from Jan. 2014 to Jun. 2019 were retrospectively analyzed, and compared with 48 patients (in the open group) who received open surgery in the same period.The operative time, intraoperative blood loss, length of hospital stay, postoperative drainage volume, postoperative parathyroid hormone, cosmetic effect, incidence of surgical complications and postoperative recurrence rate of the two groups were observed. Statistical software SPSS16.0 was used for statistical analysis of the data.Results:Both groups successfully completed the operation. Compared with the open group, the operation time ( t=11.232, P<0.001) and postoperative drainage volume ( t=2.892, P=0.003) of the robot group were more than those of the open group, and the aesthetic effect was better ( t=3.291, P=0.024). Intraoperative blood loss ( t=1.575 , P=0.118), hospital stay ( t=-1.784, P=0.091), postoperative PTH ( t=1.892, P=0.086), incidence of surgical complications ( χ2=3.291, P=0.610), visual analogue score of postoperative pain ( t=-2.431, P=0.081) and postoperative recurrence rate were not significantly different ( P>0.05). No recurrence occurred during follow-up. Conclusion:For patients with a selective benign thyroid tumor with a diameter of 5-8 cm, the bilateral axillo-breast and transaxillary approach for thyroid surgery by Da Vinci robot are safe, effective and have better cosmetic results.

18.
Article in Chinese | WPRIM | ID: wpr-856303

ABSTRACT

Objective: To compare the early effectiveness of minimally invasive open reduction and internal fixation via posterior median approach versus arthroscopic double-tunnel suture fixation in treatment of tibial avulsion fracture of the posterior cruciate ligament (PCL). Methods: A clinical data of 31 patients with the tibial avulsion fracture of the PCL and met the criteria between January 2015 and January 2019 was retrospectively analyzed. Nineteen patients (group A) were treated with open reduction and internal fixation with cannulated screw via posterior median approach. The other 12 patients (group B) were treated with arthroscopic double-tunnel suture fixation technique. There was no significant difference between the two groups ( P>0.05) in the gender, age, side of effected limb, the injury cause, the time from injury to operation, the combined meniscus injury, Meyers & McKeever classification and preoperative Lysholm score, Tegner score, International Knee Documentation Committee (IKDC) score, and the difference of tibial posterior displacement between bilateral knees. The operation time, postoperative complications, fracture healing, and the difference of tibial posterior displacement between bilateral knees, Lysholm score, Tegner score, and IKDC score were recorded. Results: Group B spent significantly longer operation time than group A ( t=7.347, P=0.000). No postoperative complication occurred in group B, and 1 patient in group A had a screw breakage. All patients were followed up 6-36 months (mean, 22 months). X-ray films showed that all fractures healed at 3 months after operation. At last follow-up, there was no significant difference in the patients with normal knee range of motion between the two groups ( P=0.510). At last follow-up, the difference of tibial posterior displacement between bilateral knees, Lysholm score, Tegner score, and IKDC score in the two groups were superior to those before operation ( P0.05). Conclusion: For the tibial avulsion fracture of PCL, the minimally invasive open reduction and internal fixation and arthroscopic double-tunnel suture fixation can obtain similar early effectiveness. However, arthroscopic surgery has the advantages of being able to simultaneously deal with intra-articular combined injuries, avoiding internal fixator complications, and eliminating the need for secondary operation.

19.
Article in Chinese | WPRIM | ID: wpr-847713

ABSTRACT

BACKGROUND: Poly(glycerol sebacate) holds excellent and good biocompatibility, flexibility and degradability, which is widely used in soft tissue replacement and tissue engineering, drug delivery carrier, wound dressing, and bone-cartilage regeneration. OBJECTIVE: To summarize the research progress in the optimal synthesis and medical application of poly(glycerol sebacate) and its composites. METHODS: PubMed, Elsevier, CNKI and WanFang databases were retrieved. The key words were “poly(glycerol sebacate), synthesis, cardiac muscle, blood vessels, nerves, skin, drug delivery carrier, wound dressing, bone regeneration” in English and Chinese, respectively. Finally, 43 articles eligible for the inclusion criteria were obtained. RESULTS AND CONCLUSION: In recent years, poly(glycerol sebacate) has attracted much attention because of its many excellent properties. Many basic scientific studies and animal experiments have confirmed that it is suitable for tissue engineering. Conventional poly(glycerol sebacate) curing process requires high temperature, high vacuum and long duration, which prevents the polymer from binding directly to cells or temperature-sensitive molecules, resulting in some limitations in its application. The composite scaffold material synthesized with a variety of other materials can make up for the corresponding shortcomings of its application in myocardial and vascular tissue engineering, drug delivery carrier, nerve guiding materials, skin and wound dressing, and bone-cartilage tissue engineering. At present, most of the studies on poly(glycerol sebacate) composites focus on the cytobiology level, and few studies focus on the mechanism of action in vivo. Further study may develop an important material for tissue replacement.

20.
Article in Chinese | WPRIM | ID: wpr-827966

ABSTRACT

Huangpu Tongqiao Capsules(HPTQC), with the functions of invigorating Qi and kidney, eliminating phlegm and removing blood stasis, have the effect of treating Alzheimer's disease(AD), but its mechanism needs further exploration. To explore the relationship between the therapeutic mechanism of HPTQC on Alzheimer's disease and EGFR-PLCγ signal pathway, 40 healthy male SD rats were selected and divided into 4 groups randomly: sham operation group(sham), model group(model), HPTQC group(HPTQC), and nimodipine group(NMP). AD rat model was established by intraperitoneal injection of D-galactose combined with an intracerebral injection of amyloid-β peptide(25-35). After 28 days of administration, Morris water maze test and HE staining showed that the learning and memory ability of AD rats were significantly decreased(P<0.01), and hippocampal neurons were obviously da-maged. However, HPTQC could improve the learning and memory ability of AD rats(P<0.05) and reduce the damage of hippocampal neurons. Immunofluorescence test results showed that the expression levels of EGFR and p-Tau in hippocampal CA1 region of AD rats were significantly increased(P<0.01), and HPTQC could reduce the expression of EGFR and p-Tau in hippocampus of AD rats(P<0.01). Western blot results showed that the protein expression levels of EGFR, PLCγ, IP3 R and p-Tau in hippocampus of AD rats were significantly increased(P<0.01), and HPTQC could reduce the protein expression of EGFR, PLCγ, IP3 R and p-Tau in AD rats(P<0.05). RT-PCR results showed that the mRNA levels of EGFR, PLCγ, IP3 R and Tau in hippocampus of AD rats were significantly increased(P<0.01), and HPTQC could reduce the mRNA levels of EGFR, PLCγ, IP3 R and Tau in AD rats(P<0.05). The results indicate that HPTQC can improve the learning and memory ability of AD rats, and its mechanism of action may be related to regulating EGFR-PLCγ signal pathway.


Subject(s)
Alzheimer Disease , Amyloid beta-Peptides , Animals , Capsules , Disease Models, Animal , ErbB Receptors , Hippocampus , Male , Rats , Rats, Sprague-Dawley , Signal Transduction
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