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1.
Acta Pharmaceutica Sinica ; (12): 1867-1879, 2023.
Article in Chinese | WPRIM | ID: wpr-978660

ABSTRACT

By integrating plant metabonomics and target quantitative analysis methods, this study systematically analyzed the differences of chemical constituents in Scutellaria baicalensis leaves from different producing areas in Shanxi, so as to provide theoretical basis for rational and effective utilization of Scutellaria baicalensis leaves. Based on the idea of plant metabonomics, the liquid quality of 53 batches of Scutellaria baicalensis leaves from 8 different producing areas in Shanxi was analyzed by UPLC-QTOF-MS, and the collected data were imported into SIMCA 14.1 software for multivariate statistical analysis to screen the different chemical constituents among different habitats in Shanxi. Meanwhile, a method for simultaneous determination of 7 flavonoids and 3 organic acids in Scutellaria baicalensis leaves was optimized and established to quantitatively analyze the differences of chemical components in Scutellaria baicalensis leaves from different producing areas in Shanxi. The results of plant metabonomics showed that there were differences in the chemical composition of Scutellaria baicalensis leaves in northern Shanxi (Datong, Xinzhou), Jinzhong (Yangquan, Luliang) and southern Shanxi (Changzhi, Yuncheng, Jincheng, Linfen): there were 14 significant differences in chemical composition between northern Shanxi and Jinzhong; there were 18 significant differences in chemical constituents between southern Shanxi and central Shanxi. There were 15 significant differences in chemical constituents between northern Shanxi and southern Shanxi. Among them, scutellarin and isocarthamidin-7-O-glucuronide were the common differences among the three regions, and the content of scutellarin was the highest in southern Shanxi and the lowest in northern Shanxi. The content of isocarthamidin-7-O-glucuronide was the highest in Jinzhong area and the lowest in northern Shanxi area. Quantitative analysis further confirmed that the average contents of apigenin, naringenin and citric acid were the highest in northern Shanxi, scutellarin, caffeic acid, apigenin-7-O-glucuronide, malic acid and wogonoside were the highest in southern Shanxi, and wogonoside and baicalin were the highest in central Shanxi. This study is of great significance to the quality control of Scutellaria baicalensis leaf resources, and provides theoretical basis for rational and effective utilization of Scutellaria baicalensis leaf resources.

2.
Article in Chinese | WPRIM | ID: wpr-1015624

ABSTRACT

Argon is the most abundant inert gas in the atmosphere. For the past few years, the biological functions of argon have been discovered and gradually recognized by scientists. Argon, as a gaseous molecule with cytoprotective functions, could positively affect the physiological and pathological processes to varying degrees. Compared with the classic gasotransmitters, argon is non-toxic, harmless, and abundant, attracting extensive attention in all professions. This review outlined the biological effects and the potential molecular mechanisms of argon, and summarized the supply methods and the positive roles of argon in neuroprotection, cardiovascular disease, inflammatory disease, organ transplantation, and other animal clinical models, and also overviewed the research progress in post-harvest preservation of agricultural products and plant tolerance to abiotic stress. The mechanisms in the above studies about the biological effects of argon were closely related to argon control of receptors, ion channel proteins, reprogramming gene expression, and re-establishment of redox and ion homeostasis. Meanwhile, argon-regulated phosphorylation and dephosphorylation modification in enzyme proteins might be crucial in argon biology. The potential applications of argon have excellent prospects in medicine and agriculture with security and cost advantages.

3.
National Journal of Andrology ; (12): 972-976, 2013.
Article in Chinese | WPRIM | ID: wpr-268021

ABSTRACT

<p><b>OBJECTIVE</b>To explore the pathogenesis of male sexual dysfunction by studying cell apoptosis and carbonate monoxide (CO) contents in the penile tissue of rats at different ages.</p><p><b>METHODS</b>We equally divided 24 male Wistar rats into an adult group (8 months old), an elderly group (16 months old) and an aged group (24 months old), and detected cell apoptosis by HE staining and TUNEL and CO levels in the penile tissues of different groups by modified dual-wavelength spectrophotometry.</p><p><b>RESULTS</b>Cell apoptosis increased with aging: 4.38 +/- 1.06 in the adult, 9.38 +/- 1.69 in the elderly and 18.50 +/- 1.60 in the aged group, while the CO content decreased with aging: 12.19 +/- 0.87 in the adult, 7.93 +/- 0.63 in the elderly and 5.68 +/- 0.62 in the aged group, both with statistically significant differences among the 3 groups (P < 0.05). The change of cell apoptosis showed a good negative correlation with that of the CO content (r = -0.889, P < 0.01).</p><p><b>CONCLUSION</b>Cell apoptosis and CO content change in the penile tissue of aging rats, and CO may participate in the development of ED by regulating cell apoptosis.</p>


Subject(s)
Animals , Male , Rats , Aging , Apoptosis , Carbon Monoxide , Metabolism , Penis , Cell Biology , Metabolism , Rats, Wistar
4.
Chinese Journal of Oncology ; (12): 490-493, 2008.
Article in Chinese | WPRIM | ID: wpr-357391

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the apoptosis-inducing effects of NNAMB, a novel polyamine conjugate, in erythroleukemia K562 cells and its molecular mechanism.</p><p><b>METHODS</b>Cell viability was assessed by MTT assay and trypan blue dye exclusion method. The cell morphology was observed by fluorescence microscopy. The cell cycle distribution, apoptosis and mitochondrial membrane potential were measured by flow cytometry. The expression of caspase-3, -8, -9, cytochrome c in the K562 cells was detected by Western blot.</p><p><b>RESULTS</b>NNAMB inhibited the proliferation of K562 cells. The cells treated with NNAMB showed a typical apoptotic morphology, Sub-G1 peak and loss of mitochondrial membrane potential. Western blot assay showed that NNAMB increased the expression of caspase-3, -9, cytochrome c but not caspase-8 in a dose-and time-dependent manner.</p><p><b>CONCLUSION</b>NNAMB induces apoptosis via mitochondrial pathway in K562 cells.</p>


Subject(s)
Humans , Anthracenes , Pharmacology , Apoptosis , Caspase 3 , Metabolism , Caspase 8 , Metabolism , Caspase 9 , Metabolism , Cell Cycle , Cell Proliferation , Cytochromes c , Metabolism , K562 Cells , Membrane Potential, Mitochondrial , Polyamines , Pharmacology , Spermidine , Pharmacology
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