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Chinese Journal of Endocrinology and Metabolism ; (12): 201-207, 2022.
Article in Chinese | WPRIM | ID: wpr-933391


Objective:To assess the change and inter-individual variation in body temperature and analyze related variables among Chinese adults.Methods:Data of of 9 184 participants[mean age(52.38±14.69) years, 4 350 men and 4 834 women] were obtained from the China Health and Nutrition Survey(CHNS) 2015. Descriptive statistics and liner regression models were deployed in the analysis.Results:Overall, the mean normal axillary body temperature was 36.43℃, higher temperature was observed in men(0.032℃, P<0.001) than women. Using the multivariate linear regression model, it was revealed that age, region, territory, season, height, and hip circumference were significantly associated with body temperature. Higher individual temperature was observed in urban(0.028℃) than rural, in southern(0.040℃) than northern, and in winter(0.054℃) than autumn. Body temperature was also negatively associated with age(-0.001℃ per year), hip circumference(-0.001℃ per cm), but is positively associated with height(0.003℃ per cm). With other variables controlled, body temperature was related to whether there is myocardial infarction(ever vs never 0.187℃). Conclusion:In China, the current normal body temperature is lower than that established in 19th century(37℃), and body temperature is related to several factors. When screening and diagnosing a disease, inter-individual variance should be fully considered.

Journal of China Pharmaceutical University ; (6): 332-338, 2021.
Article in Chinese | WPRIM | ID: wpr-881392


@#To establish a method for the determination of formaldehyde and glyoxal simultaneously in varenicline tartrate active pharmaceutical ingredient (API) and its intermediate, formaldehyde and glyoxal were derivatized by 2, 4-dinitrophenylhydrazine (2,4-DNPH) to improve the HPLC retention and UV detection sensitivity. Separation was performed on a C8 (150 mm × 4.6 mm, 5 μm) column by linear gradient elution using acetonitrile and water as the mobile phase; the detective wavelength was set at 380 nm.Formaldehyde and glyoxal were quantitatively determined by an external reference method.Linear calibration was established for both formaldehyde and glyoxal in the range from 0.094 to 1.88 μg/mL.The detection and the quantification limits were 0.047 μg/mL (19 μg/g) and 0.094 μg/mL (38 μg/g), respectively.The recoveries were (95.0±1.1)% and (99.4 ± 2.6)% for formaldehyde and glyoxal, respectively.This method has been fully validated to be applicable to quantitative analysis of trace amount of formaldehyde and glyoxal in varenicline tartrate API and its intermediate.Test results demonstrated that the contents of both formaldehyde and glyoxal met the permitted daily exposure (PDE) limits for the finished products of varenicline tartrate API as well as its intermediate, though the glyoxal contents in the crude intermediates were likely to exceed the limit.The established method is valuable for the manufacturing process and quality control of varenicline tartrate.

Journal of China Pharmaceutical University ; (6): 555-565, 2021.
Article in Chinese | WPRIM | ID: wpr-904328


@#This study aimed to identify the related substances of lorazepam tablets by liquid chromatography mass spectrometry (LC-MS). To separate the related substances of lorazepam tablets, gradient elution was performed using acetonitrile and 0.1% acetic acid -20 mmol/L of ammonium acetate as mobile phase on Inert Sustain C18 (250 mm × 4.6 mm, 5 μm).The accurate mass and elemental composition of the parent ions and their product ions of related substances were determined by electrospray-ionization quadrupole time-of-flight high resolution mass spectrometry (ESI-Q-TOF/MS).The structures of the related substances were identified by spectral analysis. Under the established analytical condition, lorazepam and its related substances were adequately separated, and 22 major related substances with content greater than 0.1% were detected and identified by hyphenated techniques in lorazepam tablets and their stressed samples.Among them, 5 were the impurities listed in the USP or EP, and the others were unknown related substances identified for the first time in this paper.The LC-MS technique can effectively separate and identify the related substances of lorazepam tablets, which provides some reference for quality control.

Journal of Modern Laboratory Medicine ; (4): 107-109,112, 2016.
Article in Chinese | WPRIM | ID: wpr-605428


Objective To understand the differences of virulence genes and molecular typing in Campylobacterjejuni isolates from poultry products and diarrhea patients in Shenzhen.Methods According to specific primers,four virulence genes (cdtB,cadF,flaA,virB1 1 )of C.jejuni were detected by polymerase chain reaction (PCR).Molecular typing for C.jejuni strains was performed by Pulsed-field gel electrophoresis (PFGE).Results There were no differences of gene distribution (cdtB+,cadF+,virB1 1-)between isolates from poultry products and diarrhea patients.Two virulence genes of cdtB and cadF were found in all of ten C.jejuni strains lacking virB1 1 .The carriage rate of flaA in food-borne isolates (3/5 )was higher than those in patient isolates (2/5).In the PFGE map,the clustering analysis of C.jejuni strains showed that a total of 5 to 9 DNA bands were observed in ten strains through the digestion of Sam I.There was high homology (above 85%) between food-borne isolates and patients isolates,but the distribution of flaA in these highly homologous strains was differ-ent.Conclusion So far,C.jejuni strains with cdtB,cadF and flaA were present in Shenzhen,and showed high diversity and homology.This implies that the occurrence of diarrhea in patients with C.jejuni was associated with the contaminated poul-try products by this pathogen.Their findings can provide basic data and evidences about diarrheal disease caused by food-borne C.jejuni for the local region.