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1.
Chinese Journal of Urology ; (12): 42-46, 2023.
Article in Chinese | WPRIM | ID: wpr-993969

ABSTRACT

Objective:To compare the efficacy of modified "double flaps" pyeloplasty and traditional dismembered pyeloplasty in the treatment of special types of hydronephrosis with small pelvis and long proximal ureteral stricture in children.Methods:The data of 39 children with special types of hydronephrosis treated in Seventh Medical Center, General Hospital of PLA from June 2018 to June 2019 were retrospectively analyzed. Among them, 33 were boys and 6 were girls. The median age of the patients was 12.0(4.5, 63.5) months. Nine of them had left hydronephrosis and four children had right hydronephrosis. These patients with small pelvis existed the characteristics that the anteroposterior diameter of pelvis was smaller than 2.5 cm and these patients existed the symptom of hematuria, flank pain or recurrent urinary tract infection with the imaging revealing ureteral obstruction. The length of proximal ureteral stenosis ranged from 2.0 to 4.0 cm. Among 39 cases, 19 cases were operated with modified "double flaps" pyeloplasty, which was the modified "double flaps" pyeloplasty group. 20 cases were operated with traditional Anderson-Hynes pyeloplasty, which was traditional pyeloplasty group. The technique of modified "double flaps" pyeloplasty mainly included that the renal pelvis was cut into double flaps, the inferior flap was anastomosed with the spatulated ureter and the superior was covered, so that the length and caliber of the ureter were partial extended. The median age of two groups were 12.0 (6.0, 44.0) months and 12.0 (4.8, 62.8) months respectively, the anterior and posterior diameter of renal pelvis were (2.8±0.8)cm and (2.6±0.6)cm respectively, and split renal function were (36.7±5.1)% and (36.0±6.8)% respectively. There were no statistically significant differences in above parameters between the two groups( P>0.05). The clinical efficacy of the two groups were compared by collecting and comparing the operation related data and postoperative follow-up data. Results:The operation of 39 children in this study was successfully completed without conversion to open surgery.The operation time of "double flap" pyeloplasty group and traditional pyeloplasty group were (142.6±9.6) min and (124.5±8.6) min respectively, and the intraoperative anastomosis time were (56.1±7.2) min and (47.6±4.8) min respectively. There were significant difference in operation time and intraoperative anastomosis time between the two groups( P<0.05). Thirty-nine children were followed up normally without loss. The mean follow-up time was (27.7±2.5) months after surgery. In the "double flaps" pyeloplasty group, 2 cases suffered with fever who were diagnosed as urinary tract infection and improved after antibiotic treatment. In the traditional pyeloplasty group, 2 cases suffered with fever who were diagnosed as urinary tract infection and improved after antibiotic treatment. Two children had flank pain during follow-up to more than one year and the examination revealed that the anteroposterior diameter of the renal pelvis gradually increased. So surgery were performed again and the two children recovered. There were no significant differences in complication rate (2/19 and 4/20) and short-term surgical success rate(19/19 and 18/20) between the two groups ( P>0.05). Conclusions:The operation time and anastomosis time of the modified "double flap" technique for treating hydronephrosis are longer than those of the traditional method. But in the treatment of special types of hydronephrosis with small renal pelvis or long proximal ureteral stricture, it may have application prospects in reducing complications.

2.
Chinese Journal of Schistosomiasis Control ; (6): 179-182, 2022.
Article in Chinese | WPRIM | ID: wpr-923780

ABSTRACT

Objective To investigate the morphological characteristics of Dermatophagoides farinae at different developmental stages. Methods The cultured D. farinae was isolated, and the external morphological features of mites at various developmental stages were observed using scanning electron microscopy (SEM), including egg, larva, nymph and adult stages. Results The D. farinae egg appeared a long oval shape, and the larval mites had three pairs of legs. The nymph had four pairs of legs and underdeveloped genital pores containing genital setae and anal setae, and adult mites appeared long and oval in shape, with decorative patterns on epidermis, and had four pairs of legs. In male adult mites, remarkable thickening of the leg I and thicker and longer leg III than the leg IV were seen, and ventral genital regions were found between the basal segments of legs III and IV; the anus was surrounded by a circular peri-anal ring, with a pair of anal suckers and anal setae within the ring. In the female adult mites, slender legs III and IV with an equal length were seen, and a “λ-shape” genital hole was observed on the ventral surface, with a crescent-like genital plate in the anterior part, and the anus appeared a longitudinal slit. Conclusions An SEM observation of the external morphology of D. farinae provides understandings of the morphological characteristics of D. farinae, which is of great significance for the classification and identification.

3.
Chinese Journal of Pediatrics ; (12): 311-316, 2022.
Article in Chinese | WPRIM | ID: wpr-935693

ABSTRACT

Objective: To investigate the relationship between body mass index (BMI) and sexual development in Chinese children. Methods: A nationwide multicenter and population-based large cross-sectional study was conducted in 13 provinces, autonomous regions and municipalities of China from January 2017 to December 2018. Data on sex, age, height, weight were collected, BMI was calculated and sexual characteristics were analyzed. The subjects were divided into four groups based on age, including ages 3-<6 years, 6-<10 years, 10-<15 years and 15-<18 years. Multiple Logistic regression models were used for evaluating the associations of BMI with sexual development in children. Dichotomous Logistic regression was used to compare the differences in the distribution of early and non-early puberty among normal weight, overweight and obese groups. Curves were drawn to analyze the relationship between the percentage of early puberty and BMI distribution in girls and boys at different Tanner stages. Results: A total of 208 179 healthy children (96 471 girls and 111 708 boys) were enrolled in this study. The OR values of B2, B3 and B4+ in overweight girls were 1.72 (95%CI: 1.56-1.89), 3.19 (95%CI: 2.86-3.57), 7.14 (95%CI: 6.33-8.05) and in obese girls were 2.05 (95%CI: 1.88-2.24), 4.98 (95%CI: 4.49-5.53), 11.21 (95%CI: 9.98-12.59), respectively; while the OR values of G2, G3, G4+ in overweight boys were 1.27 (95%CI: 1.17-1.38), 1.52 (95%CI: 1.36-1.70), 1.88 (95%CI: 1.66-2.14) and in obese boys were 1.27 (95%CI: 1.17-1.37), 1.59 (95%CI: 1.43-1.78), and 1.93 (95%CI: 1.70-2.18) (compared with normal weight Tanner 1 group,all P<0.01). Analysis in different age groups found that OR values of obese girls at B2 stage and boys at G2 stage were 2.02 (95%CI: 1.06-3.86) and 2.32 (95%CI:1.05-5.12) in preschool children aged 3-<6 years, respectively (both P<0.05). And in the age group of 6-10 years, overweight girls had a 5.45-fold risk and obese girls had a 12.54-fold risk of B3 stage compared to girls with normal BMI. Compared with normal weight children, the risk of early puberty was 2.67 times higher in overweight girls, 3.63 times higher in obese girls, and 1.22 times higher in overweight boys, 1.35 times higher in obese boys (all P<0.01). Among the children at each Tanner stages, the percentage of early puberty increased with the increase of BMI, from 5.7% (80/1 397), 16.1% (48/299), 13.8% (27/195) to 25.7% (198/769), 65.1% (209/321), 65.4% (157/240) in girls aged 8-<9, 10-<11 and 11-<12 years, and 6.6% (34/513), 18.7% (51/273), 21.6% (57/264) to 13.3% (96/722), 46.4% (140/302), 47.5% (105/221) in boys aged 9-<10, 12-<13 and 13-<14 years, respectively. Conclusions: BMI is positively correlated with sexual development in both Chinese boys and girls, and the correlation is stronger in girls. Obesity is a risk factor for precocious puberty in preschool children aged 3-<6 years, and 6-<10 years of age is a high risk period for early development in obese girls.


Subject(s)
Adolescent , Child , Child, Preschool , Female , Humans , Male , Body Mass Index , China/epidemiology , Cross-Sectional Studies , Obesity/epidemiology , Overweight/epidemiology , Puberty , Puberty, Precocious , Sexual Development
4.
Chinese Journal of Applied Clinical Pediatrics ; (24): 434-438, 2022.
Article in Chinese | WPRIM | ID: wpr-930450

ABSTRACT

Objective:To explore the incidence of non-thyroidal illness syndrome (NTIS) in children with type 1 diabetes mellitus (T1DM) and the correlation between triiodothyronine level and the severity of T1DM, thus providing evidence for clinical diagnosis and treatment.Methods:A total of 125 children initially diagnosed as T1DM at the Department of Endocrinology and Genetic Metabolism of Children′s Hospital of Shanghai Jiao Tong University from January 2015 to December 2019 were recruited.The data were retrospectively analyzed.The incidence of NTIS in T1DM children was explored.T1DM children were classified into euthyroid group and NTIS group, two independent sample t-test were used to compare the differences in multiple factors between euthyroid group and NTIS group in children with T1DM, and Spearman correlation analysis was used to further analyze the correlation between the levels of free triiodothyronine (FT3), total triiodothyronine (TT3) and the severity of children with primary T1DM. Results:The incidence of NTIS in 125 T1DM children was 26.4%(33/125 cases). FT3, TT3, total thyroxine (TT4), thyrotropin (TSH), pH value, HCO 3- and high density lipoprotein cholesterol (HDL-C) levels in NTIS group were significantly lower than those of euthyroid group [(3.10±0.45) pmol/L vs.(4.85±0.75) pmol/L, (0.60±0.28) nmol/L vs.(1.05±0.38) nmol/L, (65.77±23.41) nmol/L vs.(89.57±18.23) nmol/L, (0.91±0.89) mIU/L vs.(2.05±0.76) mIU/L, 7.21±0.17 vs.7.31±0.18, (11.49±7.54) mmol/L vs.(16.80±8.38) mmol/L, (1.08±0.49) mmol/L vs.(1.28±0.44) mmol/L]( t=4.56, 5.67, 4.48, 5.61, 2.82, 2.68, and 2.53, all P<0.05). Moreover, the anion gap (AG) level, blood glucose (BG) and triglyceride in NTIS group were significantly higher than those of euthyroid group [(22.53±8.33) mmol/L vs.(16.94±7.52) mmol/L, (24.85±4.71) mmol/L vs.(21.46±6.64) mmol/L, (2.72±2.05) mmol/L vs.(2.33±3.05) mmol/L]( t=3.22, 2.67 and 2.04, all P<0.05). The incidence of diabetic ketoacidosis (DKA) was statistically significant in euthyroid group, NTIS group and abnormal thyroid disease group (33.3% vs.63.6% vs.35.7%)( χ2=8.990, P<0.05). In T1DM children, FT3 was positively correlated with pH value and HCO 3-, and negatively correlated with AG level ( r=0.376 9, 0.439 7 and -0.411 9, all P<0.05). In addition, TT3 was positively correlated with pH value and HCO 3-, and negatively correlated with AG and BG ( r=0.513 2, 0.539 8, -0.482 4 and -0.211 5, all P<0.05). Conclusions:Children with T1DM are prone to have abnormal thyroid hormone levels, and the incidence of NTIS was 26.4%.The incidence of DKA differed in T1DM children with different thyroid functions.FT3 or TT3 level may contribute to evaluate the disease severity of T1DM children.

5.
Journal of Peking University(Health Sciences) ; (6): 526-531, 2022.
Article in Chinese | WPRIM | ID: wpr-940997

ABSTRACT

OBJECTIVE@#To investigate the introduction of vegetables and fruits in 4-8 months old infants, and to describe the maternal and infants' characteristics associated with the introduction of vegetables and fruits.@*METHODS@#Mother-infant dyads (n=228) were recruited from 12 to 16 weeks postpartum and formally entered the study at 4 months of age. Data collected via face to face interview at 4-8 months postpartum, including the timing and types of added vegetables and fruits, as well as a variety of maternal and infant characteristics (n=204). Rank sum test and multiple linear regression were used to analyze the maternal and infant characteristics related to the introduction of vegetables and fruits.@*RESULTS@#The time of introducing vegetables was concentrated at the age of 7 months, and the time of adding fruits was mainly at 6 months. Fruits were added earlier than vegetables (P < 0.001), and the variety of the added fruits was higher than that of vegetables (P=0.045). 48% (n=98) of infants had no more than three types of fruits and vegetables at 8 months. Only 9.8% (n=20) had added more than 10 kinds of fruits and vegetables at 8 months. Green leafy vegetables were the most commonly added vegetable, and apple was the most popular fruit. Compared with women who were 35 years of age or younger, women beyond 35 years old introduced vegetables to their babies 0.6 months later. 4-month-old exclusively breastfed infants had vegetables 0.4 months later than mixed-fed infants. Women with a bachelor's degree or above added 2-3 more types of fruits and vegetables to their babies than those with junior high school education and below.@*CONCLUSION@#The adding time of fruits was earlier than that of vegetable. Apples and green leafy vegetables are commonly added. Women with lower educational backgrounds add fewer types of fruits and vegetables to their babies. Mothers who choose exclusive breastfeeding and those over 35 years of age at childbirth add vegetables to their babies later than others. They should be targeted for health promotion programs that aim to improve the intake of fruits and vegetables among infants.


Subject(s)
Adult , Female , Humans , Infant , Infant, Newborn , Beijing , Breast Feeding , Follow-Up Studies , Fruit , Vegetables
6.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 77-85, 2022.
Article in Chinese | WPRIM | ID: wpr-940488

ABSTRACT

ObjectiveTo explore the differences in response to bakuchiol-induced hepatotoxicity between Institute of Cancer Research (ICR) mice and Kunming (KM) mice. MethodThe objective manifestations of bakuchiol-induced hepatotoxicity in mice were confirmed by acute and subacute toxicity animal experiments, and enrichment pathways of differential genes between normal ICR mice and KM mice were compared by transcriptomics. The real-time quantitative polymerase chain reaction (real-time qPCR) assay was used to verify the mRNA expression of key genes in the related pathways to confirm the species differences of bakuchiol-induced liver injury. ResultIn the subacute toxicity experiment, compared with the normal mice, the ICR mice showed increased serum content of alkaline phosphatase (ALP), and 5′-nucleotidase (5′-NT), without significant difference, and no manifest change was observed in KM mice. Pathological results showed that hepatocyte hypertrophy was the main pathological feature in ICR mice and hepatocyte steatosis in KM mice. In the acute toxicity experiment, KM mice showed erect hair, mental malaise, and near-death 3 days after administration. The levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in KM mice (400 mg·kg-1) significantly increased(P<0.01), and the activity of total reactive oxygen species (SOD) in liver significantly decreased(P<0.01)compared with those in normal mice, while the serum content of 5′-NT and cholinesterase (CHE) in ICR mice (400 mg·kg-1) were significantly elevated (P<0.01). The liver/brain ratio in ICR mice increased by 20.34% and that in KM mice increased by 29.14% (P<0.01). The main pathological manifestation of the liver in ICR mice was hepatocyte hypertrophy, while those in KM mice were focal inflammation, hepatocyte hypertrophy, and hepatocyte steatosis. Kyoto Encyclopedia of Genes and Genomes(KEGG)and Reactome pathway enrichment analyses showed that the differential gene expression between ICR mice and KM mice was mainly involved in oxidative phosphorylation, bile secretion, bile acid and bile salts synthesis, and metabolism pathway. CYP7A1 was up-regulated in all groups with drug intervention (P<0.01) and MRP2 was reduced in all groups with drug intervention of KM mice (P<0.01) and elevated in all groups with drug intervention of ICR mice (P<0.01) compared with those in the normal group. The expression of BSEP was lowered in ICR mice with acute liver injury (400 mg·kg-1) (P<0.05). SHP1 was highly expressed in KM mice with acute liver injury (400 mg·kg-1). The expression of FXR was diminished in ICR mice with subacute liver injury (200 mg·kg-1) (P<0.01). SOD1, CAT, and NFR2 significantly decreased in KM mice with acute liver injury (400 mg·kg-1), and CAT dwindled in KM mice with subacute liver injury (200 mg·kg-1) (P<0.01). GSTA1 and GPX1 significantly increased in KM mice with acute liver injury (400 mg·kg-1) (P<0.01) and SOD1, CAT, NRF2, and GSTA1 significantly increased in ICR mice with subacute liver injury (200 mg·kg-1) (P<0.01). CAT and NRF2 significantly increased in ICR mice with acute liver injury (400 mg·kg-1) (P<0.01). ConclusionWith the increase in the dosage of bakuchiol, the liver injury induced by oxidative stress in KM mice was gradually aggravated, and ICR mice showed stronger antioxidant capacity. The comparison of responses to bakuchiol-induced hepatotoxicity between ICR mice and KM mice reveals that ICR mice are more suitable for the investigation of the mechanisms related to bile secretion and bile acid metabolism in the research on bakuchiol-induced hepatotoxicity in mice. KM mice are more prone to liver injury caused by oxidative stress.

7.
Chinese Journal of Contemporary Pediatrics ; (12): 360-365, 2022.
Article in Chinese | WPRIM | ID: wpr-928614

ABSTRACT

OBJECTIVES@#To study the clinical efficacy of ultrasound-guided endoscopic retrograde appendicitis therapy in children with appendix-related chronic abdominal pain.@*METHODS@#A retrospective analysis was performed on the medical data of 30 children with the chief complaint of chronic abdominal pain who were admitted from August 2019 to May 2021. All the children were found to have inflammation of the appendix or intracavitary stool and fecalith by ultrasound and underwent ultrasound-guided endoscopic retrograde appendicitis therapy. The medical data for analysis included clinical manifestations, endoscopic findings, white blood cell count, neutrophil percentage, length of hospital stay, and cure rate.@*RESULTS@#Among the 30 children with chronic abdominal pain, there were 13 boys (43%) and 17 girls (57%), with a mean age of (9±3) years (range 3-15 years) at diagnosis. The median duration of the disease was 12 months, and the median length of hospital stay was 3 days. The children had a median white blood cell count of 6.7×109/L and a neutrophil percentage of 50%±13%. Fecalith and a large amount of feces were flushed out of the appendix cavity for 21 children (70%) during surgery. The follow-up rate was 97% (29/30), and the median follow-up time was 11 months (range 5-26 months). Of the 29 children, abdominal pain completely disappeared in 27 children (93%).@*CONCLUSIONS@#Ultrasound-guided endoscopic retrograde appendicitis therapy is effective in children with chronic abdominal pain caused by feces or fecalith in the appendix cavity.


Subject(s)
Adolescent , Child , Child, Preschool , Female , Humans , Male , Abdominal Pain/etiology , Appendicitis/surgery , Appendix/surgery , Fecal Impaction , Retrospective Studies , Ultrasonography, Interventional
8.
Chinese Journal of Applied Clinical Pediatrics ; (24): 1648-1650, 2021.
Article in Chinese | WPRIM | ID: wpr-908032

ABSTRACT

Objective:To summarize clinical features, diagnosis, treatment, and follow-up of children with pseudohypoaldosteronism type 1 (PHA1) and review relevant literatures to improve the understanding of the disease and reduce misdiagnosis.Methods:Six children with the main performance of salt losing treated in the Shanghai Children′s Hospital from January 2015 to December 2018, who were diagnosed as PHA1 after relevant auxiliary examinations and genetic tests.They were classified and analyzed for their treatment courses and follow-up prognosis.Results:Six children with PHA1 had varying degrees of salt losing, dehydration and infection.After the examination, 3 cases with urinary system malformations were diagnosed as secondary PHA1.Genetic testing of 2 cases revealed 2 hete-rozygous mutations c. 1439+ 1G>C and c. 875+ 1G>A in the intron region of the SCNN1A gene, and they were diagnosed as multiple target organ defect/systemic PHA1 according to American College of Medical Genetics and Genomics(ACMG) guidelines.The other case failed to be examined by genetic testing due to the refusal of parents, and was finally diagnosed as renal PHA1 according to clinical diagnosis and treatment.Conclusions:PHA1 is a rare cause of infant salt-losing syndrome, renal and secondary PHA1 children can recover quickly after sodium supplementation and the secondary factors are removed; while multiple target organ defect/systemic PHA1 has severe clinical manifestations, electrolyte imbalance is not easy to correct, and fatal arrhythmia is prone to occur, the mortality rate is high.It is easy to be misdiagnosed in clinical practice.Auxiliary examination and genetic testing can help to diagnose and classify PHA1, as well as individualized treatment.

9.
International Journal of Pediatrics ; (6): 559-563, 2021.
Article in Chinese | WPRIM | ID: wpr-907279

ABSTRACT

The regulatory mechanisms of pubertal development are complex and affected by many factors.MicroRNAs, which major participants in epigenetics, are short single-stranded RNAs composed of 21-25 nucleotides.They can regulate the expression or translation of multiple target genes, playing an important role in complex biological phenomena.During pubertal development, microRNAs can regulate the expression of related genes on the hypothalamic-pituitary-gonadal axis(HPGA). Recently, studies have found that the lack or overexpression of some specific microRNAs can cause abnormal pubertal development(premature or delayed)and lead to reproductive disorders, providing a new direction for the diagnosis and treatment of puberty diseases.Here we mainly review the regulatory mechanisms that microRNAs participate in pubertal development.

10.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 171-183, 2021.
Article in Chinese | WPRIM | ID: wpr-906253

ABSTRACT

Objective:This research aims to construct the "disease-gene-target-components-drug" network with the methods of network pharmacology and bioinformatics, and to explore the key genes and signaling pathways of Xiao Qinglongtang in the treatment of bronchial asthma. Method:First,we selected the differentially expressed genes between patients with asthma and healthy people with use of the gene expressing Omnibus(GEO) database,and searched the active ingredients from Xiao Qinglongtang with use of TCMSP database,and then screened disease genes and herb ingredient targets as intersecting genes to construct the protein-protein interactions (PPI) network by using R language and Cytoscape 3.7.2 software. At the same time Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were carried out. Result:Series GSE43696 in GEO database were successfully filtered,which contained 108 pieces of chip data. A total of 820 differentially expressed genes were screened from the chip data. Then we filtered 169 active ingredients and 246 targets of Xiao Qinglongtang from database. Through the above steps,we obtained 25 intersecting genes, and PPI network results showed that 91 potential targets may be involved in the mechanism of Xiao Qinglongtang. A total of 180 gene functions such as response to oxidative stress,inflammatory response,extracellular matrix organization and positive regulation of vascular endothelial growth factor production were showed in GO enrichment analysis results. 39 signaling pathways were showns in the results of KEGG pathway enrichment analysis,such as T helper cell 17(Th17) cell differentiation,interleukin 17(IL-17)signaling pathway,tumor necrosis factor(TNF) signaling pathway,and hypoxia inducible factor-1(HIF-1) signaling pathway. Conclusion:Xiao Qinglongtang fully embodies the characteristics of multi-components,multi-targets and multi-pathways in the intervention of bronchial asthma. The results of the study could provide an important basis for mechanism research of Xiao Qinglongtang in treating asthma.

11.
Shanghai Journal of Preventive Medicine ; (12): 1026-1030, 2021.
Article in Chinese | WPRIM | ID: wpr-905810

ABSTRACT

Objective:To analyze the epidemiological characteristics of 8 clusters of coronavirus disease 2019 (COVID-19) in Chenzhou City, and provide scientific basis for epidemic prevention and control. Methods:Descriptive epidemiological analysis was conducted for 8 COVID-19 clusters, comparing and analyzing the differences of infection rates among close contacts within and outside the family, and emphatically describing two typical cases. Results:8 COVID-19 clusters were reported in Chenzhou with a total of 31 cases from January to February, 2020. The main source of infection of the family index cases was Hubei Province. Cough symptoms were observed in 67.74% of the cases, followed by fever (54.84%). The infection rate of close contacts within the family (55.00%) was higher than that outside the family (2.56%), and the difference was statistically significant (χ2=28.177, P<0.001). The infection rate of spouse of the family index cases was 85.71%, higher than that of parents (77.78%), other family members (44.44%) and children (40.00%), and the difference was not statistically significant (χ2=6.004, P=0.120). Two typical cases suggested that both COVID-19 pre-symptomatic and asymptomatic patients have the potential to excrete the virus from the body and become sources of infection. Conclusion:Effective family prevention and control measures and early sampling and screening of people in key epidemic areas are conducive to early detection, early isolation and early treatment of infected people, so as to avoid the occurrence and spread of family clusters.

12.
Journal of Experimental Hematology ; (6): 1101-1108, 2021.
Article in Chinese | WPRIM | ID: wpr-888524

ABSTRACT

OBJECTIVE@#To screen the core genes of Philadelphia chromosome positive/Ph like T-cell acute lymphoblastic leukemia (Ph@*METHODS@#The WES/RNA-seq examination results of Ph@*RESULTS@#For Ph@*CONCLUSION@#There are obviously abnormal DNA damage repair pathways in children with Ph


Subject(s)
Child , Humans , Computational Biology , Gene Expression Profiling , Gene Expression Regulation, Neoplastic , Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/genetics , Signal Transduction , Software
13.
National Journal of Andrology ; (12): 815-818, 2021.
Article in Chinese | WPRIM | ID: wpr-922163

ABSTRACT

Objective@#To evaluate the clinical efficacy of Compound Chamomile and Lidocaine Hydrochloride Gel for postoperative hypospadias in children.@*METHODS@#From January to December 2020, we treated 116 children with distal hypospadias in the Department of Urology, Department of Pediatrics and the Seventh Medical Center of the PLA General Hospital, 58 by primary Snodgrass urethroplasty only (the control group) and the other 58 with Compound Chamomile and Lidocaine Hydrochloride Gel smeared on the penis postoperatively in addition (the trial group). We compared the operation time and postoperative pain score, edema regression and incidence of infection between the two groups, followed by statistical analysis using T test and Chi-square test.@*RESULTS@#All the operations were successfully completed by the same surgeon under general anesthesia. There were no statistically significant differences between the trial and control groups in age ([2.5 ± 0.8] vs [2.4 ± 0.6] yr, P > 0.05) or operation time ([95.6 ± 14.5] vs [97.1 ± 15.2] min, P > 0.05). No incision infection occurred in any of the cases. The pain scores at dressing removal were remarkably lower in the trial than in the control group at 2 hours (1.4 ± 1.0 vs 2.6 ± 1.3, P < 0.05), 24 hours (2.2 ± 1.3 vs 3.9 ± 1.6, P < 0.05), 48 hours (1.2 ± 0.7 vs 1.6 ± 0.9, P < 0.05) and 72 hours after surgery (2.5 ± 0.8 vs 3.7 ± 1.8, P < 0.05). Significantly more cases of edema regression were achieved in the trial than in the control group at 2 weeks postoperatively (35 vs 19, P < 0.05).@*CONCLUSIONS@#Compound Chamomile and Lidocaine Hydrochloride Gel can effectively relieve pain, reduce edema and accelerate edema regression after surgery in children with hypospadias, and therefore deserves wide clinical application.、.


Subject(s)
Child, Preschool , Humans , Male , Chamomile , Hypospadias/surgery , Lidocaine/therapeutic use , Pain, Postoperative/drug therapy , Postoperative Period
14.
China Journal of Chinese Materia Medica ; (24): 2413-2423, 2021.
Article in Chinese | WPRIM | ID: wpr-879142

ABSTRACT

To probe the potential hepatotoxic components of Epimedii Folium and investigate its mechanism based on network toxicology and cell experimental validation. According to the previous results of component measurement and cytotoxicity evaluation, 11 active compounds related to hepatotoxicity in Epimedii Folium were chosen as research object in this study. Through SwissTargetPrediction database and GeneCards database, the potentially hepatotoxic targets of Epimedii Folium were obtained. Subsequently, the protein-target interaction network and active compounds-hepatotoxic targets network were established to analyze the core targets and screen the key hepatotoxic compounds in Epimedii Folium. Meanwhile, the signaling pathways and molecular mechanisms were inferred with GO functional enrichment analysis and KEGG pathway enrichment analysis on the core targets. At last, the effect of icaritin as the chief hepatotoxic compound on the indexes related to hepatotoxicity in HL-7702 cells and HepG2 cells was investigated to validate the hepatotoxicity mechanism of Epimedii Folium. Through the network toxicology analysis, 190 action targets and 991 hepatotoxic targets were collected, then 64 potentially hepatotoxic targets of Epimedii Folium including AKT1, EGFR, MAPK3, TNF and so on were obtained, and icaritin was screened as the key hepatotoxic compound. GO functional enrichment analysis indicated 160 biological process terms such as protein phosphorylation and negative regulation of apoptotic process, 41 molecular function terms such as protein binding and ATP binding, and 32 cellular component terms such as cytosol and cell surface. KEGG pathway enrichment analysis inferred 75 signaling pathways involving PI3 K-Akt and HIF-1. After comprehensive analysis, it was inferred that the hepatotoxicity mechanism of Epimedii Folium was related with regulating oxidative stress and apoptosis. The results of cell biology experiments showed that icaritin could significantly increase the level of aspartate aminotransferase and lactate dehydrogenase, reduce the level of glutathione, improve the quality of reactive oxygen species and reduce mitochondrial membrane potential, indicating that it could cause hepatotoxicity by destroying cell membrane structure, inhibiting antioxidant enzyme activity, activating oxidative stress and inducing apoptosis. These results proved the reliability of results of network pharmacology. This study preliminarily clarified the material base and the mechanism of potential hepatotoxicity of Epimedii Folium, which provided important information for further research and safe application.


Subject(s)
Drugs, Chinese Herbal/toxicity , Plant Leaves , Protein Interaction Maps , Reproducibility of Results
15.
Chinese Journal of Urology ; (12): 856-860, 2020.
Article in Chinese | WPRIM | ID: wpr-869762

ABSTRACT

Objective:To evaluate the safety and feasibility of robotic-assisted laparoscopic resection of bladder rhabdomyosarcoma.Methods:A retrospective study was performed in 4 children underwent robotic-assisted surgery for bladder rhabdomyosarcoma from July 2018 to September 2019 in our hospital. The perioperative information and short time outcomes were collected. All the four patients were male. The average age of these patients was 68 months (from 11 to 122 months). After cystoscopy biopsy was conducted, preoperative chemotherapy was carried out. Preoperative radiotherapy was also added depends on the tumor response, then robotic-assisted surgery was performed. Radical cystectomy and orthotopic neobladder reconstruction with bilateral ureter reimplanataion were carried out for the bladder was widely occupied case. Bladder preserving surgery was performed for the other three localized cases.Results:All of the four operations have been conducted successfully without conservation to open surgery. The mean operative time was 189(104-316) min while the mean estimated blood loss was 32.5(20-50) ml. The mean tumor diameter was 4.48(2.7-6.0)cm. According to the pathological results, all of the four cases were diagnosed as embryonal rhabdomyosarcoma. One case had positive margin while other three cases had negative margins. No complication more than Clavien-Dindo grade Ⅲ was observed. The mean length of hospital stay was 18(14-24)days and the mean follow-up time was 14.7(7-21) months. Postoperative chemotherapy was carried out according to the protocol of IRS low risk group for all cases (VAC, eight cycles). Postoperative radiotherapy was conducted for the case with positive margin. No recurrence was recorded during the follow-up period.Conclusions:Robotic-assisted laparoscopic technique for the treatment of bladder rhabdomyosarcoma in children is safe and feasible.

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Chinese Journal of Urology ; (12): 531-535, 2020.
Article in Chinese | WPRIM | ID: wpr-869701

ABSTRACT

Objective:To discuss the efficacy of robotic-assisted laparoscopic hemi-nephrectomy for the treatment of complete duplex kidney.Methods:We retrospectively reviewed the clinical data of the 20 patients who had robotic-assisted laparoscopic hemi-nephrectomy surgery from March 2017 to November 2019. Four of these patients were male, while the others were female.The average age of these patients was 28.6 months, ranged from 1-2 months.The duplex kidneys were on the upper pole, 9 on the left side, and l1 on the right side. All the patients had a history of gradual exacerbations of hydronephrosis before surgery. The most commonly seen symptoms were lumbar/abdominal pain(2 cases), dysuresia(1 case) and leakage of urine(1 case). Twelve patients had a history of repeated urinary tract infections. One patient expressed the cystic mass of the urethra protruded from the external orifice. Preoperative diagnosis was confirmed by magnetic resonance urinary water imaging (MRU), diuretic renal dynamic imaging(ECT), intravenous pyelography(IVP), and voiding cystoureterography(VCUG). Two cases were of ectopic urete, 4 of ureterocele and 4 of vesieoureteral reflux(VUR). All the patients underwent hemi-nephrectomy for the treatment of complete duplex kidney. Then we observed the intraoperative and postoperative complications and follow-ups.Results:A11 the cases performed by robotic-assisted laparoscopic surgery transperitoneally were completed successfully.There was no significant complication during the surgery with quick recovery after the surgery.The mean operative time was 109.5min(range 82-150 min). The mean estimated blood loss was about 9 ml (range 5-22 ml). Routinely placing a peripelvic catheter drainage was recommended. The mean hospital stay was 5.9 days(range 4-8 days). One patient was lost to follow-up.Mean follow-up was 13.6 months (range 3 -30 months). The results of the re-examination of ECT did not indicate loss or atrophy of lower renal function 3 months after operation. Primary symptoms improved.Conclusions:Robotic-assisted laparoscopic hemi-nephrectomy for hydronephrosis with duplex kidney could be minimal invasive and effective, and has achieved similar results with cosmetic scar .

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Chinese Journal of Perinatal Medicine ; (12): 604-609, 2019.
Article in Chinese | WPRIM | ID: wpr-756155

ABSTRACT

Objective To assess the influences of early implementation of patient-controlled epidural analgesia (PCEA) in labor on uterine myoelectrical activity and delivery outcomes. Methods A prospective study was conducted on 240 singleton cephalic primiparae with spontaneous labor at Guangzhou Women and Children's Medical Center from January 2015 to October 2018. Those women, who were ready to accept PCEA, were randomly assigned to early- or late-PCEA group based on cervical dilation of 0-3 cm or 3-6 cm at the time of commencing PCEA, while those who refused PCEA in labor were classified as non-PCEA group. Uterine electromyographic activity and visual analogue score (VAS)were recorded before and 1 h and 2 h after PCEA. Patient satisfaction with labor, duration of the first stage of labor, volume of postpartum bleeding within 2 h after delivery and neonatal Apgar score were compared between different groups using multivariate analysis of variance, repeated measures analysis of variance, LSD-t test or Chi-square test. Results The VAS values 1 h after PCEA in the early- and late-PCEA group were both lower than that in the non-PCEA group (2.08±1.34 and 2.00±1.28 vs 7.65±1.04, LSD-t were - 27.713 and - 27.663, P<0.001) and those before PCEA (7.65±0.91 and 7.62±0.86, LSD-t were -32.879 and -33.349, P<0.001). The VAS values 2 h after PCEA in the early- and late-PCEA group were both lower than that in the non-PCEA group (1.63±1.53 and 1.41±1.56 vs 7.66±0.87, LSD-t were -27.018 and -27.823, P<0.001) and those before PCEA (LSD-t were -31.379 and -32.718, P<0.001).The patient satisfaction rate with labor was higher in the early-PCEA group comparing to the late-PCEA group [80.0% (72/90) vs 61.1% (55/90), P<0.001], and the two figures above were both higher than that of the non-PCEA group [20.0% (12/60), both P<0.001]. There was no significant difference in the duration of the first stage of labor, the volume of postpartum blood loss 2 h after delivery, oxytocin usage rate, the rate of convertion to cesarean section, neonatal birth weight or Apgar score at 1 or 5 min among the three groups (all P>0.05). There was also no significant difference in uterine electromyographic parameters among the three groups before or 2 h after PCEA (all P>0.05). The number and duration of burst, power density spectrum peak frequency, root mean square and total power 1 h after PCEA in the early- and later-PCEA group were all lower than those in the non-PCEA group [4.80±2.49 and 5.54±3.04 vs 9.67±2.44; (34.41±1.21) and (36.94±1.18) vs (41.68±1.53) s; (0.36±0.08) and (0.36±0.07) vs (0.48±0.05) Hz ; (0.05±0.04) and (0.05±0.05) vs (0.07±0.05) mV; (4.33±0.51) and (5.36 ±0.59) vs (9.90±1.43) pV2; all P<0.05]. Conclusions The effect of PCEA on uterine myoelectrical activity has no association with the commencing time. While early PCEA could alleviate the labor pain as soon as possible, which enable us to improve the efficacy of labor analgesia, patient satisfaction and maternal and neonatal safety without increasing cesarean section rate.

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Chinese Journal of Applied Clinical Pediatrics ; (24): 574-577, 2019.
Article in Chinese | WPRIM | ID: wpr-743514

ABSTRACT

Thyroid disease is one of the most common diseases in pregnant women.Thyroid diseases during pregnancy mainly include hypothyroidism and hyperthyroidism.Thyroxine in mothers flows throughout the placenta throughout pregnancy.Thyroxine plays an important role in fetal development,especially in brain development.In the early stages of pregnancy,thyroid hormones required for fetal growth and development are entirely dependent on the mother.Untimely treatment or poor control of thyroid diseases during pregnancy will not only increase the risk of adverse pregnancy complications,but also adversely affect the intelligence development and thyroid function of fetuses and neonates.Therefore,attention should be paid to the diagnosis and treatment of thyroid diseases during pregnancy in order to reduce the harm to future generations.In addition,for neonates born to mothers with thyroid diseases during pregnancy,attention should be paid to physical examination,assessment of intellectual development and monitoring of thyroid function are required.

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Chinese Journal of Disease Control & Prevention ; (12): 1155-1157, 2019.
Article in Chinese | WPRIM | ID: wpr-779483

ABSTRACT

Objective To investigate the infestation status and environment tolerance of ornithonyssus bacoti (O. bacoti) in stored traditional Chinese herb Semen Coicis (Coix seed). Methods 800 g of stored Semen Coicis was initially taken, and subjected to passing a 40-mesh sampling sieve. Then 80 g of Coix seed particles was sampled to directly isolate the mites that were made into slide specimen in conventional technique, and identified as the procedures described in previous literature. Results A total of 449 mites were isolated from the 80 g stored Semen Coicis particles, and were identified as O. bacoti. Conclusions O.bacoti was first detected in the stored traditional Chinese medicinal Semen Coicis with which infestation is serious. This status should call out attention to prevent the disease associated with transmission of pathogens from O.bacoti.

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Chinese Journal of Schistosomiasis Control ; (6): 513-515, 2019.
Article in Chinese | WPRIM | ID: wpr-818981

ABSTRACT

Objective To observe the morphological characteristics of live Carpoglyphus lactis at different stages of life cycle and its ultrastructure. Methods The live C. lactis specimens were isolated from longan pulp, and firstly rinsed with double distilled water under a light microscope to make slide preparation by the conventional manner, and then were made into scanning electron microscope (SEM) specimens. The specimens were observed under SEM for the ultrastructure characteristics at different developmental stages including egg, larva, nymph and adult (male and female) . Results The SEM photographs showed that the egg was oval and milky. The larva had three pairs of legs, without genital setae, preanal seta and coxal rod, and there was no trace of genital growth. The nymph had four pairs of legs, genital seta and preanal seta, whereas the genital area was looked still under-developed. The male adult had a conical gnathosoma and a pair of external verticals which were longest setae at the dorsal idiosoma, whereas the other dorsal setae were all short rhabdoid. At the ventral idiosoma, there was an aedoeagus which was like a bent cube, and the top was straight forward. The external sacral setae and post anal were longest setae. The female adult’s genital plates were a composite of plastron and cutex inner root, covering the genital tract, and a hole of anus and a pair of anal setae were located at posterior of idiosoma. Conclusion The morphological characteristics and ultra-structure of C. lactis at different stages of life cycle can be vividly observed under SEM, which provides the morphological bases for further study of the relation between parasitism and disease.

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