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1.
Article in Chinese | WPRIM | ID: wpr-939713

ABSTRACT

OBJECTIVE@#To investigate the safety and efficacy of a new proteasome inhibitor Ixazomib followed by autologous hematopoietic stem cell transplantation (AHSCT) in the treatment of POEMS syndrome.@*METHODS@#The clinical manifestations, diagnosis and treatment process and follow-up results of 4 patients with POEMS syndrome who were treated with Ixazomib-based regimen combined with AHSCT in Wuhan No.1 Hospital from February 2018 to July 2020 were analyzed retrospectively. All patients were male, aged from 37-54 years old, with varying degrees of peripheral neuropathy, organ enlargement (liver, spleen or lymph nodes), circulatory overload (peripheral edema and/or pleural effusion), osteosclerosis, endocrine diseases (thyroid, gonads, etc.), skin changes (pigmentation, hemangioma, white nails, etc.), M protein, papilledema and other clinical manifestations and characteristics at the time of initial treatment. Two patients were pathologically diagnosed as hyaline vascular Castleman disease by lymph node biopsy. Three patients underwent lumbar puncture examinations and all showed elevated cerebrospinal fluid protein. All patients received at least 2 cycles of sequential AHSCT after induction chemotherapy based on ixazomib. The follow-up time was 10-28 months, and the median follow-up time was 16 months.@*RESULTS@#All cases survived. The complications were controllable during the treatment. Moreover, the clinical symptoms related to the disease were improved to a certain extent after the treatment. The levels of vascular endothelial growth factor (VEGF) showed a gradual decline.@*CONCLUSION@#Ixazomib combined with AHSCT is safe and effective in the treatment of POEMS syndrome.


Subject(s)
Adult , Boron Compounds , Glycine/analogs & derivatives , Hematopoietic Stem Cell Transplantation , Humans , Male , Middle Aged , POEMS Syndrome/therapy , Retrospective Studies , Transplantation, Autologous , Vascular Endothelial Growth Factor A
2.
Chinese Journal of Pathology ; (12): 419-424, 2022.
Article in Chinese | WPRIM | ID: wpr-935556

ABSTRACT

Objective: To investigate the tumor immunity-related pathologic features and clinical significance in pancreatic ductal adenocarcinoma (PDAC). Methods: All pathologic materials and clinical information of 192 PDAC patients from the Cancer Hospital of the University of Chinese Academy of Sciences from January 2010 to December 2020 were collected. The onco-immune microenvironment associated morphologic features were evaluated, and MHC-Ⅰ, PD-L1, CD3, and CD8 expression were detected by immunohistochemistry (IHC). Then the correlation between the factors and their influence on prognosis was analyzed. Results: There were 163 cases of non-specific adenocarcinoma (163/192, 84.90%), 18 cases of adeno-squamous carcinoma (18/192, 9.37%), and 11 cases of other rare subtypes (11/192, 5.73%). Perineural invasion was observed in 110 cases (110/192, 57.29%) and vascular invasion in 86 cases (86/192, 44.79%). There were 84 cases (84/182, 46.15%) with severe chronic inflammation. Tumor infiltrating immune cell numbers (TII-N) were increased in 52 cases (52/192, 27.08%). Lymphocytes and plasma cells were the main infiltrating immune cells in 60 cases (60/192, 31.25%), whereas in 34 cases (34/192, 17.71%) the tumors were mainly infiltrated by granulocytes, and 98 cases (98/192, 51.04%) showed mixed infiltration. CD3+T cells were deficient in 124 cases (124/192, 66.31%). CD8+T cells were deficient in 152 cases (152/192, 79.58%). MHC-Ⅰ expression was down-regulated in 156 cases (156/192, 81.25%), and PD-L1 was positive (CPS≥1) in 46 cases (46/192, 23.96%). Statistical analysis showed that TII-N was negatively correlated with vascular invasion (P=0.035), perineural invasion (P=0.002), stage (P=0.004) and long-term alcohol consumption (P=0.039). The type of immune cells correlated positively with chronic pancreatic inflammation (P=0.002), and negatively with tumor differentiation (P=0.024). CD8+T cells were positively correlated with CD3+T cells (P=0.032), MHC-Ⅰ expression (P<0.001) and PD-L1 expression (P=0.001), and negatively correlated with long-term smoking (P=0.016). Univariate analysis showed that histological nonspecific type (P=0.013) and TII-N (P<0.001) were the factors for good prognosis. Vascular invasion (P=0.032), perineural invasion (P=0.001), high stage (P=0.003) and long-term alcohol consumption (P=0.004) were adverse prognostic factors. COX multivariate risk analysis found that TII-N was an independent favorable factor for PDAC, while perineural invasion was an independent adverse risk factor. Conclusions: TII-N is an independent superior prognostic factor for PDAC, and significantly correlated with many factors; chronic alcohol consumption and smoking may inhibit onco-immunity in PDAC patients.


Subject(s)
Adenocarcinoma/pathology , B7-H1 Antigen/metabolism , Biomarkers, Tumor/metabolism , Carcinoma, Pancreatic Ductal/pathology , Humans , Inflammation/pathology , Lymphocytes, Tumor-Infiltrating/metabolism , Pancreatic Neoplasms/pathology , Prognosis , Tumor Microenvironment
3.
Chinese Journal of Pathology ; (12): 332-337, 2022.
Article in Chinese | WPRIM | ID: wpr-935535

ABSTRACT

Objective: To assess the clinical features and treatment outcomes in patients with primary ovarian squamous cell carcinoma (POSCC). Methods: Fifteen patients with primary ovarian squamous cell carcinoma diagnosed from January 2009 to December 2018 in Cancer Hospital of the University of Chinese Academy of Sciences were collected. The expression of p16, hMLH1, hMSH2, hMSH6 and PMS2 in POSCC was detected by immunohistochemistry, and the status of high-risk human papillomavirus (HPV) by RNAscope test. Results: Squamous cell carcinoma with different degrees of differentiation was found in 15 cases, including three cases with high differentiation and 12 cases with medium to low differentiation. There were four cases with in situ squamous cell carcinoma, four cases with teratoma, one case with endometrial carcinoma/atypical hyperplasia, and one case with endometriosis. p16 was expressed in five cases (5/15), indicating coexisting high-risk HPV infection. There was no high-risk HPV infection in the remaining 10 cases, and p16 staining was negative. There was no deficient mismatch repair protein in all cases. The overall survival time (P=0.038) and progression free survival (P=0.045) of patients with high-risk HPV infection were longer than those without HPV infection. Conclusions: POSCC is more commonly noted in postmenopausal women and often occurs unilaterally. Elevated serological indexes CA125 and SCC are the most common finding. Morphologically, the tumors show variable degrees of differentiation, but the current data suggest that the degree of differentiation cannot be used as an independent prognostic index. High-risk HPV infection may be associated with the occurrence of POSCC, and that the prognosis of POSCC patients with HPV infection is better than that of patients without infection.


Subject(s)
Carcinoma, Squamous Cell/pathology , Cyclin-Dependent Kinase Inhibitor p16/analysis , Female , Humans , Immunohistochemistry , Papillomavirus Infections/diagnosis , Prognosis
4.
Article in Chinese | WPRIM | ID: wpr-932582

ABSTRACT

Objective:To evaluate the efficacy and safety of the combination of conventional western medicine therapy and oral traditional Chinese medicine (TCM) compound in the prevention and treatment of radiotherapy-inducedoral mucositis (RTOM) of nasopharyngeal carcinoma patients treated with concurrent radiotherapy and chemotherapy.Methods:A randomized, single-center, and open-label controlled experiment was conducted. Software Stata was used to generate random numbers, and 100 subjects were randomly assigned to two groups ata 1∶1 ratio, namely the integrated Chinese and western medicine group(the integrated group) and the conventional western medicine group. This study focused on the incidence of level-ⅢRTOM, followed by these verity degree of RTOM, therisk of malnutrition, and safety.Results:The incidences of level-III RTOM in the integrated group and the conventional western medicine group were 18% and 46%, respectively, with a statistically significant difference ( χ2=9.007, P=0.003). Compared to the integrated group, the conventional western medicine group showed a significantly increase dseverity degree of RTOM ( OR=3.269, 95% CI: 1.627-6.567, P<0.001) and higher risk of malnutrition ( OR=3.021, 95% CI: 1.786-5.109, P<0.001). Moreover, compared to the integrated group, the conventional western medicine group showed decrease dincidence of thirst (48.97% and 72.00% respectively; χ2=5.493, P=0.019) and decreased incidence of neutrophilcount reduction(12.24% and 30.00%, respectively, χ2=4.668, P=0.031). The incidence of mild/moderate adverse events related to TCM compound was 4.08%(2/49), and no serious adverse events related to TCM compound were observed. Conclusions:Compared to the conventional western medicine regimen, the integrated Chinese and western medicine regimen IS more effective in the prevention and treatment of RTOM. Meanwhile, its clinical application is safe and reliable.

5.
Article in Chinese | WPRIM | ID: wpr-906039

ABSTRACT

Epilepsy is a common nervous system disorder characterized by repeated attacks and a protracted course, which can cause great harms to the physical and mental health of patients. Antiepileptic drugs have been proved effective, but the resulting toxic and side effects cannot be ignored. Traditional Chinese medicine (TCM) has a long history of dealing with epilepsy. At present, in addition to enriching the cognitive theory of epilepsy treatment with TCM, we have also focused on the role of TCM in regulating the epilepsy-related signaling pathways from the perspective of molecular biology. The review of literature in China and abroad has uncovered that epilepsy is closely related to such pathophysiological processes as cell proliferation, apoptosis, autophagy, inflammatory response, and immune response. At the same time, the modern research of Chinese and western medicines shows that the efficacy of Chinese herbal monomers, single Chinese herbs or Chinese herbal compounds in treating epilepsy is directly or indirectly related to their regulation of signaling pathways. To be specific, they control epileptic seizures and alleviate epileptic brain injury by regulating the expression of key molecules in corresponding signaling pathways. This paper summarized the research progress in China and abroad as follows: ①Tangeretin and ginkgolide B inhibit apoptosis and oxidative stress by activating the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway. ②Baicalin and osthol suppress autophagy by inhibiting the mammalian target of rapamycin (mTOR) signaling pathway. ③Ganoderan and astragaloside reduce apoptosis by inhibiting the mitogen-activated protein kinase (MAPK) signaling pathway. ④Salidroside and resveratrol reverse oxidative stress and apoptosis by activating the nuclear factor E2-related factor 2/antioxidant reaction element/heme oxygenase 1 (Nrf2/ARE/HO-1) signaling pathway. ⑤Curcumin and baicalin diminish inflammatory response and apoptosis by inhibiting the nuclear transcriptional factor-κB (NF-κB) signaling pathway. The above summary is expected to provide reference for the in-depth study and clinical application of TCM for the treatment of epilepsy.

6.
Article in Chinese | WPRIM | ID: wpr-905816

ABSTRACT

Objective:To understand the school absence due to illnesses in the context of emergency response to COVID-19 in Minhang District, so as to provide a basis for epidemic prevention and control in primary and secondary schools. Methods:Descriptive epidemiological analysis was conducted on the data of school absence due to illnesses in primary and secondary schools in Minhang District in the context of emergency response to COVID-19. Results:During the period of emergency response to COVID-19, the incidence of illness-induced absenteeism in Minhang District was 1.50%. Difference in absenteeism between different months was statistically significant (χ2=1 724.31, P<0.01). The monthly absenteeism rate in 2020 was higher than that from 2016 to 2019, and the difference was statistically significant (P<0.01). The rate of symptom-induced absenteeism was 1.28%. Fever was the most common symptom of school absence, and the rate of absence due to fever in 2020 was higher than that of the same period in 2019 and 2016-2018, and the difference was statistically significant (χ2=15 281.33, P<0.01). Acute upper respiratory tract infection was the number one cause of illness-induced absence during the emergency response period, while infectious diseases were the primary causes of diseases from 2016 to 2018 and in 2019. The difference of infectious disease absence in different years was statistically significant (χ2=1 822.62, P<0.01). Conclusion:The rate of school absence due to illnesses increased significantly in the context of emergency response to COVID-19 in Minhang District, while the rate of school absence due to infectious diseases decreased significantly. The prevention and control measures during the epidemic period can be used for reference in the future for preventing infectious diseases under normal conditions.

7.
Acta Pharmaceutica Sinica ; (12): 3362-3369, 2021.
Article in Chinese | WPRIM | ID: wpr-906836

ABSTRACT

Isopentenyl diphosphate isomerase (IDI) is a key enzyme in the regulation of triterpenes biosynthesis and plays an important role in ginsenoside biosynthesis. In this study, two IDI genes, PvfIDI1 (GenBank No. MZ736417) and PvfIDI2 (GenBank No. MZ736418) were cloned from Panax vietnamensis var. fuscidiscus. The open reading frame of both PvfIDI1 and PvfIDI2 was 924 bp encoding 307 amino acids. The molecular weights of PvfIDI1 and PvfIDI2 were 34.84 kDa and 34.66 kDa, respectively, with theoretical pIs of 6.01 and 5.66. Bioinformatic analysis indicated that PvfIDI1 and PvfIDI2 contained two conserved sequences: TNTCCSHPL and WGEHELDY. Phylogenetic analysis showed that PvfIDI1 and PvfIDI2 were closely related to Panax notoginseng IDI. Expression analysis showed that both PvfIDI1 and PvfIDI2 genes are expressed in root, rhizome, stem and leaf of P. vietnamensis var. fuscidiscus. However, PvfIDI1 is highly expressed in the rhizome and PvfIDI2 is highly expressed in the stem. PvfIDI1 and PvfIDI2 recombinant proteins were expressed in E. coli; a functional coloration experiment showed that PvfIDI1 and PvfIDI2 could promote the accumulation of lycopene, indicating that both PvfIDI1 and PvfIDI2 encode functional IDI enzymes. The cloning and functional studies on PvfIDI1 and PvfIDI2 provide a foundation for the further study of IDI and the regulation of ginsenoside biosynthesis in P. vietnamensis var. fuscidiscus.

8.
Article in Chinese | WPRIM | ID: wpr-888051

ABSTRACT

Screening suitable reference genes is the premise of quantitative Real-time PCR(qRT-PCR)for gene expression analysis. To provide stable reference genes for expression analysis of genes in Aconitum vilmorinianum, this study selected 19 candidate re-ference genes(ACT1, ACT2, ACT3, aTUB1, aTUB2, bTUB, 18S rRNA, UBQ, eIF2, eIF3, eIF4, eIF5, CYP, GAPDH1, GAPDH2, PP2A1, PP2A2, ACP, and EF1α) based on the transcriptome data of A. vilmorinianum. qRT-PCR was conducted to profile the expression of these genes in the root, stem, leaf, and flower of A. vilmorinianum. The Ct values showed that 18S rRNA with high expression level and GAPDH2 with large expression difference among organs were not suitable as the reference genes. NormFinder and geNorm showed similar results of the expression stability of the other candidate reference genes and demonstrated PP2A1, EF1α, and CYP as the highly stable ones. However, BestKeeper suggested EF1α, ACT3, and PP2A1 as the top stable genes. In view of the different results from different softwares, the geometric mean method was employed to analyze the expression stability of the candidate re-ference genes, the results of which indicated that PP2A1, EF1α, and ACT3 were the most stable. Based on the comprehensive analysis results of geNorm, NormFinder, BestKeeper, and geometric mean method, PP2A1 and EF1α presented the most stable expression in different organs of A. vilmorinianum. PP2A1 and EF1α were the superior reference genes for gene expression profiling in different organs of A. vilmorinianum.


Subject(s)
Aconitum , Gene Expression Profiling , Genes, Plant/genetics , Real-Time Polymerase Chain Reaction , Reference Standards , Reverse Transcriptase Polymerase Chain Reaction
9.
International Eye Science ; (12): 297-299, 2020.
Article in Chinese | WPRIM | ID: wpr-780602

ABSTRACT

@#AIM:To investigate the clinical effect and influencing factors of phacoemulsification in cataract patients with high myopia.<p>METHODS:A retrospective analysis of 109 eyes of 90 patients with cataract combined with high myopia was performed on January 2016. According to the best corrected visual acuity, the curative effect was evaluated and divided into two groups: low vision group(best corrected visual acuity <0.3)and normal vision group(best corrected visual acuity ≥0.3). A Logistic regression analysis model was established to analyze the factors affecting the postoperative efficacy.<p>RESULTS:There were 22 cases(27 eyes)in the low vision group and 68 cases(82 eyes)in the normal vision group. During the follow-up period, 6 eyes of the posterior lens capsule became cloudy, all of which were mildly cloudy and improved after laser treatment. No complications such as secondary glaucoma, corneal decompensation, retinal detachment, and intraocular lens displacement were observed. Univariate analysis found that the axial length, corneal astigmatism, Emery classification, fundus lesion classification were the influential factors affecting the efficacy of the operation(<i>P</i><0.05). Logistic regression analysis results showed that the axial length(<i>OR</i>=2.121), corneal astigmatism(<i>OR</i>=1.698), Emery classification(<i>OR</i>=1.901), and fundus lesion classification(<i>OR</i>=1.964)were independent factors affecting the postoperative efficacy(<i>P</i><0.05).<p>CONCLUSION: Axial length, corneal astigmatism, Emery classification, and fundus lesion classification can affect cataract phacoemulsification surgery for cataract combined with high myopia, but patients have more influential factors for prognosis, and effective measures should be formulated according to influencing factors Intervention to improve the prognosis of patients.

10.
Article in Chinese | WPRIM | ID: wpr-865046

ABSTRACT

Objective:To investigate the emergency surgical strategies for patients with acute abdomen during the Corona Virus Disease 2019 (COVID-19) outbreak.Methods:The retrospective and descriptive study was conducted. The clinical data of 20 patients with acute abdomen who were admitted to the Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology between January 18, 2020 and February 10, 2020 were collected. There were 13 males and 7 females, aged from 25 to 82 years, with an average age of 57 years. All the patients with emergency surgeries received pulmonary computed tomography (CT) examination before surgery, and completed nucleic acid detection in throat swab if necessary. Patients excluded from COVID-19 underwent regular anesthesia, suspected and confirmed cases were selected a proper anesthesia based on their medical condition and surgical procedure. Patients excluded from COVID-19 underwent emergency surgeries following the regular procedure, suspected and confirmed cases underwent emergency surgeries following the three-grade protection.Observation indicators: (1) surgical situations; (2) postoperative situations. Measurement data with normal distribution were represented as average (range). Count data were described as absolute numbers.Results:(1) Surgical situations: of the 20 patients with acute abdomen, 16 patients were excluded from COVID-19, and 4 were not excluded. All the 20 patients underwent emergency abdominal surgeries successfully, of whom 2 received surgeries under epidural anesthesia (including 1 with open appendectomy, 1 with open repair of duodenal bulbar perforation), 18 received surgeries under general anesthesia (including 9 with laparoscopic repair of duodenal bulbar perforation, 3 with open partial enterectomy, 3 with laparoscopic appendectomy, 1 with laparoscopic left hemicolectomy, 1 with laparoscopic right hemicolectomy, 1 with cholecystostomy). The operation time of patients was 32-194 minutes, with an average time of 85 minutes. The volume of intraoperative blood loss was 50-400 mL, with an average volume of 68 mL. (2) Postoperative situations: 16 patients excluded from COVID-19 preopratively were treated in the private general ward postoperatively. One of the 16 patients had fever at the postoperative 5th day and was highly suspected of COVID-19 after an emergency follow-up of pulmonary CT showing multiple ground-glass changes in the lungs. The patient was promptly transferred to the isolation ward for treatment, and results of nucleic acid detection in throat swab showed double positive. Medical history described by the patient showed that the patient and family members were residents of Wuhan who were not isolated at home during the epidemic. There was no way to confirm whether they had a history of exposure to patients with COVID-19. Medical staffs involved in this case did not show COVID-19 related symptoms during 14 days of medical observation. The other 15 patients recovered well postoperatively. The 4 patients who were not excluded from COVID-19 preoperatively based on medical history and results of pulmonary CT examination were directly transferred to the isolation ward for treatment postoperatively. They were excluded from COVID-19 for two consecutive negative results of nucleic acid detection in the throat swab and recovered well. Two of the 20 patients with acute abdomen had postoperative complications. One had surgical incision infection and recovered after secondary closure following opening incision, sterilizing and dressing, the other one had intestinal leakage and was improved after conservative treatment by abdominal drainage. There was no death in the 20 patients with acute abdomen.Conclusions:Patients with acute abdomen need to be screened through emergency forward. Patients excluded from COVID-19 undergo emergency surgeries following the regular procedure, and patients not excluded from COVID-19 undergo emergency surgeries following the three-grade protection. The temperature, blood routine test and other laboratory examinations are performed to monitor patients after operation, and the pulmonary CT and throat nucleic acid tests should be conducted if necessary. Patients excluded from COVID-19 preopratively are treated in the private general ward postoperatively, and they should be promptly transferred to the isolation ward for treatment after being confirmed. Patients who are not excluded from COVID-19 preoperatively based on medical history should be directly transferred to the isolation ward for treatment postoperatively.

11.
Article in Chinese | WPRIM | ID: wpr-876247

ABSTRACT

Objective To investigate the etiology and epidemiological characteristics of diarrhea in Minhang District of Shanghai from 2014 to 2016, so as to provide scientific basis to monitor diarrhea disease and to control cluster epidemics. Methods The diarrhea cases in monitoring hospitals from 2014 to 2016 were analyzed by epidemiological methods.Stool specimens were collected using systematic sampling for etiological examination.All specimens were tested for cholera, Shigella, salmonella, Vibrio parahaemolyticus, rotavirus, norovirus and enteric adenovirus. Results Totally 11 742 cases of diarrhea were reported in Minhang District of Shanghai from 2014 to 2016.The majority of the patients were young and middle aged adults.885 stool samples were detected for pathogen, 454 cases (51.30%) were pathogen positive including 57 cases of bacterial and virus co-infection.The detection rates of bacteria and viruses were respectively 10.36%(92/454)and 34.46%(305/454).Three kinds of bacteria and five kinds of viruses were detected, that were mainly Vibrio parahaemolyticus (n=72), salmonella (n=19), norovirus (n=195) and rotavirus A (n=71).The positive rate of pathogens reached the peak from January to March.There was a significant difference in pathogen positive rate between different seasons, while no difference between different years, gender and age groups.The epidemic peaks of different pathogens were different, with bacteria in summer and virus in winter and spring. Conclusion The positive rate of pathogens that caused diarrhea in Minhang District shows a significant seasonal peak.Norovirus, Vibrio parahaemolyticus and rotavirus A are the three main pathogens.It is necessary to take effective measures for diarrhea disease control according to the epidemiological characteristics.

12.
Chinese Journal of Dermatology ; (12): 704-709, 2020.
Article in Chinese | WPRIM | ID: wpr-870350

ABSTRACT

Objective:To evaluate the proliferative activity of and changes in the expression of related differentiation proteins in a stably NCSTN gene-silenced human immortalized keratinocyte cell line HaCaT, and to preliminarily explore the possible mechanism underlying the occurrence of acne inversa.Methods:By lentivirus-mediated short hairpin RNA (shRNA) , a NCSTN gene-silenced HaCaT cell model was established (shRNA group) , and other HaCaT cells transfected with empty lentivirus served as a negative control group. Real-time quantitative PCR and Western blot analysis were performed to determine the NCSTN gene-silencing efficiency. Cell counting kit-8 (CCK8) assay was conducted to evaluate the proliferative activity of HaCaT cells, and real-time quantitative PCR and Western blot analysis were performed to determine the mRNA and protein expression of cytokeratins (CK1, CK5, CK7, CK10, CK14, CK16, CK17, CK18, CK19 and CK20) and other differentiation molecules (involucrin and loricrin) respectively in HaCaT cells. Two-independent-sample t test was used to compare the measurement data between two groups. Results:NCSTN mRNA and protein expression were significantly lower in the shRNA group (0.42 ± 0.19, 0.30 ± 0.07 respectively) than in the negative control group (1.00 ± 0.34, 1.00 ± 0.26; t = 5.196, 2.637, P < 0.001, < 0.05, respectively) , and the gene-silencing efficiency was 70%. Compared with the negative control group, the shRNA group showed higher cellular proliferative activity, but decreased protein expression of CK16, CK19 and terminal differentiation molecule involucrin ( t = 3.787, 3.817, 2.904, P < 0.01, < 0.05, < 0.05, respectively) . Conclusion:Stable silencing of NCSTN gene can lead to abnormal proliferation and differentiation of HaCaT cells, which provides new ideas for subsequent exploration of acne inversa caused by NCSTN gene mutation.

13.
Article in Chinese | WPRIM | ID: wpr-878401

ABSTRACT

Pregnancy epulis is a tumor-like lesion with high prevalence in China. The local lesion, the general condition of the pregnant patient, and the complications during treatment should be taken into consideration when making a treatment plan for pregnancy epulis. In this study, three representative pregnancy epulis cases were presented, and related studies at home and aboard were reviewed to summarize the etiology, differential diagnosis, treatment, and prevention of pregnancy epulis and share the clinical experience in the treatment of pregnancy epulis.


Subject(s)
China , Diagnosis, Differential , Female , Gingival Diseases/diagnosis , Gingival Neoplasms , Humans , Pregnancy , Prevalence
14.
Journal of Breast Cancer ; : 185-195, 2019.
Article in English | WPRIM | ID: wpr-764270

ABSTRACT

PURPOSE: Cancer cells develop acquired resistance induced by chemotherapeutic drugs. In this study, we investigated the effects of brief treatment with cytotoxic drugs on the phenotype of breast cancer cells. METHODS: Breast cancer cells MCF7 and BT-474 were briefly treated with paclitaxel or doxorubicin. Clonogenic, migration, and invasion assays were performed on the treated cells. Western blot analysis and RhoA activity assay were also performed. RESULTS: Breast cancer cells when briefly treated with paclitaxel or doxorubicin showed reduced clonogenic ability. Doxorubicin, but not paclitaxel, augmented cell migration and invasion. The invasion-promoting effects of doxorubicin were lost when the two drugs were sequentially used in combination. Myosin light chain (MLC) 2 phosphorylation and RhoA activity were upregulated by doxorubicin and downregulated by paclitaxel. Pretreatment with RhoA inhibitors abolished the migration- and invasion-promoting effects of doxorubicin. CONCLUSION: Doxorubicin activates the RhoA/MLC pathway and enhances breast cancer cell migration and invasion. Therefore, this pathway might be explored as a therapeutic target to suppress anthracycline-enhanced tumor progression.


Subject(s)
Blotting, Western , Breast Neoplasms , Breast , Cell Movement , Doxorubicin , Myosin Light Chains , Paclitaxel , Phenotype , Phosphorylation , Up-Regulation
15.
Journal of Experimental Hematology ; (6): 1912-1918, 2019.
Article in Chinese | WPRIM | ID: wpr-781519

ABSTRACT

OBJECTIVE@#To explore the effect of the expression regulation of mitotic control protein DIS3 on the proliferation ability of 3 cell Lines of human myeloma.@*METHODS@#Human myeloma cell lines NCI-h929, RPMI 8226 and U266B1 were selected as study objects, and the over-expression vector of DIS3 gene and DIS3-siRNA were designed and constructed, respectively. The cell experiments were divided into 5 groups: control, DIS3 over-expression-empty vector, DIS3-siRNA negative control, DIS3 over-expression and DIS3-siRNA group. After culture for 24 h, 48 h and 72 h, the proliferation capacity of these three cell lines was measured by MTT assay. And cell samples were collected after culture for 72 h, the expression of proliferation cell nuclear antigen (PCNA) was detected by RT-qPCR and Western blot.@*RESULTS@#MTT assay results showed that the proliferation capacity of cells in DIS3 over-expression group was significantly reduced, as compared with the DIS3 over-expression-empty vector group at the same time point (24, 48 or 72 h) (P<0.05, P<0.01). Compared with DIS3-siRNA negative control group at the same time point (24, 48 or 72 h), the proliferation capacity of cells in the DIS3-siRNA group significantly increased (P<0.05, P<0.01). The results of RT-qPCR and Western blot showed that the mRNA and protein expression levels of PCNA in cells of DIS3 over-expression group were significantly reduced, as compared with DIS3 over-expression empty vector group (P<0.01). Compared with the DIS3-siRNA negative control group, the mRNA and protein expression of PCNA in the cells of DIS3-siRNA group very significantly increased (P<0.01).@*CONCLUSION@#Over expression of DIS3 can significantly reduce the proliferation ability of 3 cell lines of human myeloma, which may be closely related with reducing PCNA expression.


Subject(s)
Cell Line, Tumor , Cell Proliferation , Exosome Multienzyme Ribonuclease Complex , Humans , Multiple Myeloma , RNA, Small Interfering , Transfection
16.
Article in Chinese | WPRIM | ID: wpr-802659

ABSTRACT

Objective@#To analyze the predictive value of serum carcinoembryonic antigen(CEA) level combined with primary PET/CT metabolic parameters, metabolic tumor volume(MTV) and total glycolysis(TLG) in liver metastasis of colorectal cancer.@*Methods@#The clinical data of 86 patients with colorectal cancer who underwent PET/CT examination in the People's Hospital of Zhejiang Province from January 2013 to December 2017 were retrospectively analyzed.Univariate and multivariate logistic regression was used to analyze the relationship between clinicopathological parameters, MTV, TLG and liver metastasis.@*Results@#Of the 86 patients, there were 17 cases(19.77%) of liver metastases.Univariate analysis showed that there were significant differences in T stage(χ2=8.83), tumor location(χ2=5.43) and serum CEA content(t=11.65) between the liver metastasis group and the non-liver metastasis group(all P<0.05). The levels of TLG[(101.94±20.14)g] and MTV[(14.09±3.25)cm3] in the liver metastasis group were significantly lower than those in the non-liver metastasis group[(135.95±22.63)g, (25.09±4.33)cm3](t=5.66, 9.80, all P<0.01). Multivariate logistic regression analysis showed that T stage(OR=3.56, 95%CI: 1.06-12.00), tumor location(OR=1.38, 95% CI: 1.05-1.81), TLG(OR=1.68, 95% CI: 1.11-2.54), MTV(OR=3.86, 95% CI: 1.63-9.14) and serum CEA(OR=2.95, 95% CI: 1.60-5.41) were the influencing factors of liver metastases in patients with colorectal cancer(all P<0.05).@*Conclusion@#T stage, tumor location, primary PET/CT metabolic parameters(TLG, MTV) and serum CEA levels are the influencing factors of liver metastasis in patients with colorectal cancer, suggesting that the detection of serum CEA level combined with primary PET/CT metabolic parameters has certain predictive value for liver metastasis of colorectal cancer.

17.
National Journal of Andrology ; (12): 223-230, 2019.
Article in Chinese | WPRIM | ID: wpr-816806

ABSTRACT

Objective@#To investigate the incidence of chromosome polymorphisms and their influence on semen quality and sperm DNA integrity in male patients receiving in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI).@*METHODS@#We retrospectively analyzed the chromosomal karyotypes and the types and incidence rate of chromosome polymorphisms in 2 370 male patients undergoing IVF/ICSI between June 2016 and June 2018. We classified the patients into groups A (with variation in the secondary constriction region in the autosomal long arm), B (with variation in the short arm of the D/G group chromosomes), C (with interbrachial inversion of chromosome 9) and D (with Y chromosome polymorphisms), and compared the semen parameters and sperm DNA fragmentation indexes (DFI) between the patients with chromosome polymorphisms and those with normal chromosomes.@*RESULTS@#Totally, 154 (6.50%) of the patients undergoing IVF/ICSI were found with chromosome polymorphisms, including 34 cases of secondary constriction variation in the long arm of the autosome (1.43% [34/2 370], 22.08% [34/154]), 82 cases of short arm polymorphisms of the D/G group chromosomes (3.46% [82/2 370], 53.25% [82/154]), 26 cases of interbrachial inversion of chromosome 9 (1.10% [26/2 370], 16.88% [26/154]), 10 cases of Y chromosome polymorphisms (0.42% [10/2 370], 6.50% [10/154]), and 2 cases of mixed chromosome polymorphisms (0.08% [2/2 370], 1.42% [2/154]). The total sperm count was lower in group D than in the other polymorphism groups and the normal chromosome group, but with no statistically significant difference among the five groups (P > 0.05). The sperm progressive motility was also lower in group D than in the other five groups, with statistically significant difference from group B (27.5 ± 13.5 vs. 41.5 ± 21.1, P = 0.027), but not from the other groups (P > 0.05). No statistically significant difference was observed in the sperm DFI between the polymorphism groups and the normal chromosome group (P > 0.05), or among the polymorphism groups (P > 0.05). The proportion of normal semen was lower in group D than in the other four groups, but with no statistically significant difference among the five groups (P > 0.05). The incidence rate of asthenospermia was higher in group D than in the other four groups, but with no statistically significant difference among the five groups (P > 0.05), and so was that of oligoasthenospermia, with statistically significant difference from the normal chromosome group (30.0% vs 8.0%, P = 0.041), but not from the other polymorphism groups (P > 0.05).@*CONCLUSIONS@#Short arm polymorphisms of the D/G group chromosomes are the most common type of chromosome polymorphisms in male patients undergoing IVF/ICSI. Polymorphisms of the Y chromosome have a negative effect on semen quality, while those of the other chromosomes do not significantly affect semen quality and sperm DNA integrity.

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Article in Chinese | WPRIM | ID: wpr-734918

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Objective To report the spontaneous remission and induced remission of phospholipase A2 receptor (PLA2R)-associated idiopathic membranous nephropathy (IMN) in adults,as well as to explore the potential prognostic factors.Methods A total of 120 patients with IMN in Huashan Hospital during 2012 and 2017 were enrolled and their clinical data were collected.Results PLA2R-associated IMN patients accounted for 89.2% of the IMN patients.Spontaneous remission occurred in 35.5% of PLA2R-associated IMN patients.The patients with higher serum albumin and lower level of PLA2R antibody were more likely to achieve spontaneous remission (both P < 0.05).Multivariate logistic regression analysis showed that male was an independent risk factor for spontaneous remission in PLA2R-associated IMN patients (OR=0.060,95%CI 0.007-0.493,P=0.009),while higher serum albumin at baseline (OR=1.480,95% CI 1.144-1.932,P=0.004) and the improvement of serum albumin after 3 months' non-immunosuppressive treatment (OR=2.040,95%CI 1.322-3.151,P=0.001) were independent protective factors for spontaneous remission.About 42.1% PLA2R-associated IMN patients had received immunosuppressive therapy,with induced remission rate being 70.7%.High serum albumin before treatment was an independent protective factor for induced remission (OR=1.268,95% CI 1.014-1.585,P=0.038).Conclusions PLA2R-associated IMN accounts for most of the IMN patients,with a spontaneous remission rate of 35.5%,during the follow-up period,which is even higher in patients with higher baseline serum albumin and lower PLA2R antibody titer.Induced remission rate is 70.7% in patients in need of immunosuppresants.The serum albumin level may be helpful in predicting spontaneous remission and response to immunosuppressive therapy.

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Article in Chinese | WPRIM | ID: wpr-753669

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Objective To analyze the predictive value of serum carcinoembryonic antigen ( CEA) level combined with primary PET/CT metabolic parameters,metabolic tumor volume( MTV) and total glycolysis( TLG) in liver metastasis of colorectal cancer.Methods The clinical data of 86 patients with colorectal cancer who underwent PET/CT examination in the People's Hospital of Zhejiang Province from January 2013 to December 2017 were retrospectively analyzed.Univariate and multivariate logistic regression was used to analyze the relationship between clinicopathological parameters,MTV, TLG and liver metastasis. Results Of the 86 patients, there were 17 cases (19.77%) of liver metastases.Univariate analysis showed that there were significant differences in T stage( χ2 =8.83),tumor location(χ2 =5.43) and serum CEA content(t=11.65) between the liver metastasis group and the non-liver metastasis group(all P<0.05).The levels of TLG[(101.94 ±20.14)g] and MTV[(14.09 ±3.25)cm3] in the liver metastasis group were significantly lower than those in the non-liver metastasis group[(135.95 ± 22.63) g, (25.09 ± 4.33)cm3] ( t=5.66,9.80,all P<0.01).Multivariate logistic regression analysis showed that T stage ( OR=3.56,95%CI:1.06-12.00),tumor location(OR=1.38,95% CI:1.05-1.81),TLG(OR=1.68,95% CI: 1.11-2.54),MTV(OR=3.86,95% CI:1.63-9.14) and serum CEA( OR=2.95,95% CI:1.60-5.41) were the influencing factors of liver metastases in patients with colorectal cancer( all P<0.05).Conclusion T stage, tumor location,primary PET/CT metabolic parameters(TLG,MTV) and serum CEA levels are the influencing factors of liver metastasis in patients with colorectal cancer,suggesting that the detection of serum CEA level combined with primary PET/CT metabolic parameters has certain predictive value for liver metastasis of colorectal cancer.

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Article in Chinese | WPRIM | ID: wpr-753406

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Objective To investigate the effect of situational simulation combined with problembased learning (PBL) in nursing interns in Department of Emergency.Methods A total of 122 nursing interns in the Department of Emergency in our hospital from June 2016 to December 2017 were selected and divided into control group (n=60) and experimental group (n=62) according to the order of internship.The interns in the control group were taught with the conventional clinical teaching mode,i.e.,the traditional teaching method,and clinical teaching was consistent with the practice time,teaching plan,teaching goal,and teaching progress of nursing interns.The interns in the experimental group were taught with situational simulation combined with PBL,and this clinical teaching mode was used for the teaching and management of nursing interns according to the contents of the syllabus and the requirements for internship.The two groups were compared in terms of comprehensive ability (theoretical assessment + skill operation assessment) and critical thinking ability based on Critical Thinking Disposition Inventory-Chinese Version (CTDI-CV).All the data were analyzed by SPSS 17.0,t test and chi-square test were used for statistical analysis.Results Compared with the control group,the experimental group had significantly better scores of comprehensive ability (89.69 ± 7.82) vs.(76.86 ± 6.12) and critical thinking ability (290.69 ± 17.83) vs.(276.67 ± 18.82).The comparison of the scores on each dimension showed that compared with the control group,the experimental groups had significantly better scores of truth-seeking [(40.23 ± 5.92) vs.(38.88 ± 6.78),P<0.05],open-mindedness [(41.92 ± 4.28) vs.(39.42 ± 6.78),P<0.05],analytical tendencies [(43.12 ± 5.12) vs.(41.22 ± 4.86),P<0.05],and inquisitiveness [(43.42 ± 4.69) vs.(40.45 ± 6.21),P<0.05].Conclusion Situational simulation combined with PBL can improve the comprehensive ability of clinical nursing interns,enhance their critical thinking ability,and improve their autonomy and enthusiasm to participate in study.

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