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1.
J. appl. oral sci ; 29: e20201031, 2021. tab, graf
Article in English | LILACS | ID: biblio-1250189

ABSTRACT

Abstract Fixed orthodontic appliances may lead to biofilm accumulation around them that may increase caries risk. This study aimed to evaluate the influence of quaternary ammonium methacrylates (QAMs) on the physicochemical properties, cytotoxicity, and antibacterial activity of adhesive resins for orthodontic purposes. Methodology: A base resin was prepared with a comonomer blend and photoinitiator/co-initiator system. Two different QAMs were added to the base adhesive: dimethylaminododecyl methacrylate at 5 wt.% (DMADDM) or dimethylaminohexadecyl methacrylate (DMAHDM) at 10 wt.%. The base adhesive, without QAMs, (GC) and the commercial Transbond™ XT Primer 3M (GT) were used as control. The resins were tested immediately and after six months of aging in the water regarding the antibacterial activity and shear bond strength (SBS). The antibacterial activity was tested against Streptococcus mutans via metabolic activity assay (MTT test). The groups were also tested for the degree of conversion (DC) and cytotoxicity against keratinocytes. Results: The resins containing QAM showed antibacterial activity compared to the commercial material by immediately reducing the metabolic activity by about 60%. However, the antibacterial activity decreased after aging (p<0.05). None of the groups presented any differences for SBS (p>0.05) and DC (p>0.05). The incorporation of DMADDM and DMAHDM significantly reduced the keratinocyte viability compared to the GT and GC groups (p<0.05). Conclusion: Both adhesives with QAMs showed a significant reduction in bacterial metabolic activity, but this effect decreased after water aging. Lower cell viability was observed for the group with the longer alkyl chain-QAM, without significant differences for the bonding ability and degree of conversion. The addition of QAMs in adhesives may affect the keratinocytes viability, and the aging effects maybe decrease the bacterial activity of QAM-doped materials.


Subject(s)
Orthodontic Brackets , Streptococcus mutans , Materials Testing , Biofilms , Resin Cements , Dental Cements , Methacrylates , Anti-Bacterial Agents
2.
Rev. Cient. CRO-RJ (Online) ; 6(2): 24-30, 2021.
Article in English | LILACS, BBO | ID: biblio-1357504

ABSTRACT

Objective: To assess the superficial microhardness of enamel-restorations margins of glass ionomer cement reinforced with silver (RS), modified with resin (RI) and composite resin (CO) after cariogenic biofilm. Materials and Methods: Thirty bovine enamel blocks with standard cavities were divided into three groups according to the materials used: RI (Riva Light Cure™, SDI), RS (Riva Silver™, SDI) and CO (Filtek™ Z350 XT, 3M). Half of each enamel block surface was covered by acid resistant varnish. After that, the blocks were exposed to Streptococcus mutans biofilm. The varnish was removed from the blocks and superficial microhardness (MDS) was measured (Knoop, 50 g, 15 s), with five indentations, 100 µm from each other in three different directions. The data were analyzed by the Shapiro Wilk, Kruskal Wallis and Mann Whitney tests (p<0.05). Results: MDS analysis indicated that in 50 µm distance from the restoration, RS group obtained hardness gain (6.31±0.01), unlike RI (-0.36±0.05) and CO (-11.43±0.02) groups that demonstrated significant loss (p<0.05). In other distances did not observe statistical difference between the groups. Regardless of the distance up to 450 µm, significant high total mineral gain was observed for RS group compared to the CO group; however, RS and RI presented similar enamel microhardness. Conclusion: All glass ionomers increased microhardness of enamel blocks even in contact with cariogenic biofilm. Although only the silver reinforced glass ionomer prevented demineralization at the margin restorations in 50 µm from the margin.


Objetivo: Comparar a desmineralização nas margens da interface dente/restauração utilizando cimento de ionômero de vidro modificado com resina (RI) e reforçado com prata (RS) e com resina composta (CO) após desafio cariogênico. Materiais e Métodos: 30 blocos de esmalte bovino com cavidades padronizadas foram divididos em 3 grupos de acordo com os materiais utilizados: RI (Riva Light CureTM, SDI), RS (Riva SilverTM, SDI) e CO (FiltekTM Z350 XT, 3M). Metade de cada superfície de esmalte restaurada foi protegida com verniz ácido-resistente. Os blocos foram expostos ao biofilme de Streptococcus mutans. O verniz foi removido dos espécimes com algodão e álcool para mensuração da microdureza superficial (MDS - Knoop, 50 g, 15 s), através de 3 linhas com 5 indentações em cada e 100 µm de distância entre elas. Os dados foram submetidos ao programa SPSS 20.0, teste de normalidade de Shapiro Wilk, Kruskal Wallis e Mann Whitney (p<0,05). Resultados: A análise da MDS demonstrou que na distância de 50 µm da restauração, o grupo RS apresentou ganho percentual de dureza (6,31 ± 0,01), diferentemente dos grupo RI (-0,036 ± 0,05) e CO (-11,43 ± 0,02) que apresentaram perda significativa (p<0,05). Nas demais distâncias, não foi observada diferença estatística entre os grupos. Conclusão: Todos os cimentos de ionômero de vidro aumentaram a microdureza superficial total dos blocos de esmalte mesmo após exposição ao biofilme cariogênico. No entanto, apenas o grupo RS impediu a desmineralização a 50 µm das margens de restaurações submetidas a biofilme cariogênico.


Subject(s)
Dental Materials , Composite Resins , Dental Enamel , Glass Ionomer Cements , Hardness Tests
3.
Rev. Cient. CRO-RJ (Online) ; 5(2): 65-68, May-Aug. 2020.
Article in English | LILACS, BBO | ID: biblio-1254134

ABSTRACT

Introduction: Studies of twins are important because environmental and genetic factors seem to be related to the phenotypic alterations. Objective: This paper presents a unique case of monozygotic twins with mirror image of a retained primary central incisor. Case report: Twin male brothers, 9-years-old, presented prolonged retention of the primary central upper incisor. The over-retained teeth in one twin were a mirror image of those in the other twin. The first twin presented a prolonged retention of the tooth 51 whereas the other twin presented a prolonged retention of tooth 61. After radiographic exams the over-retained teeth were extracted. Conclusion: Twins may show similarity in pattern of dental anomalies supporting the influence of genetic factors. In identical twins the location of diagnosed anomalies can be mirror imaged. This fact should lead the professional to examine the pair of twins in order to diagnose any dental anomaly that may be present.


Introdução: Estudos feitos em gêmeos são importantes porque fatores ambientais e genéticos parecem estar relacionados às alterações fenotípicas. Objetivo: Este artigo apresenta um caso raro de gêmeos monozigóticos apresentando imagem em espelho de retenção prolongada de incisivos centrais decíduos superiores homólogos. Relato do caso: Os irmãos gêmeos, com 9 anos de idade, não apresentavam história de trauma orofacial ou doença comum da infância. Após exames clínicos e radiográficos foram identificados a retenção do dente 51 no gêmeo 1 e do dente 61 no gêmeo 2. Em ambos os pacientes, os dentes 11 e o 21 estavam em erupção. O tratamento proposto foi a exodontia dos dentes decíduos com anestesia local e acompanhamento. Conclusão: Gêmeos podem apresentar semelhança no padrão de anomalias dentárias devido à influência de fatores genéticos. Adicionalmente, em gêmeos monozigóticos, a localização das anomalias diagnosticadas pode se apresentar invertidas ou imagem em espelho. Esse fato deve estimular o profissional a examinar o par de gêmeos para diagnosticar qualquer anomalia dentária que possa estar presente. O diagnóstico precoce e tratamento adequados devem ser realizados para evitar danos funcionais e estéticos em pacientes com retenções dentárias.


Subject(s)
Humans , Male , Child , Tooth, Deciduous/diagnostic imaging , Tooth, Unerupted/diagnostic imaging , Twins, Monozygotic , Incisor/abnormalities , Tooth, Deciduous/surgery , Tooth Extraction , Tooth, Unerupted/surgery
4.
Rev. Cient. CRO-RJ (Online) ; 4(1): 87-91, Jan.-Apr. 2019.
Article in English | LILACS, BBO | ID: biblio-1024320

ABSTRACT

Introduction: Candida albicans is one of the microrganisms that most often colonizes the oral cavity of HIV-infected children. This fungus secretes organic acids, which decrease the pH of the oral cavity; an environment that is already particularly acidic in HIV-Infected children because of their hypercaloric diets, use of sugary medicines, and poor oral hygiene. Considering the large number of dental restorations and the high prevalence of caries in this population, these conditions, including the metabolism of C. Albicans , can potentially cause problems in terms of the surface of restorative materials. Objective : Therefore, the aim of this study was to evaluate, in vitro , the potential of C. Albicans isolated from the dental biofilm of HIV-infected children to cause surface demineralization of the restorative materials used in pediatric dentistry. Material and method : Forty-four blocks of four different materials (2 resins, 1 compomer, and 1 glass ionomer cement) were made and separated into four groups (n = 11). All blocks were submitted to initial surface microhardness (ISM) analysis. Subsequently, each block was exposed to C. Albicans biofilm, formed from a 1 mL standard suspension containing 10 5 yeasts/mL, over seven days. The blocks were then cleaned and kept at 4 °C until being submitted for measurement of the final surface microhardness (FSM). The Mann-Whitney test was used for intragroup comparisons between ISM and FSM values. Results : The percentage of microhardness loss (% MHL) values between the four groups were compared using the Kruskall- Wallis test (95% CI). The ISM values ranged from 63.54 ± 11.41 to 77.92 ± 10.91, with no statistical differences being found (p = 0.76). After exposure to biofilm, no significant changes in surface microhardness were observed when comparing the values of ISM and FSM, except for group 3 (compomer Vitremer TM ), which had an FSM value of 40.45 ± 7.57 (p = 0.001). The % MHL of the compomer (group 3) was significantly higher (41.16%) than the other groups (5.35% group 1; 7.02% group 2; and 9.57% group 3) (p = 0.036). Conclusion : It can be concluded that, in vitro , C. albicans isolated from the dental biofilms of HIV-infected children can cause significant reduction in the surface microhardness of compomer compared with other materials.


Introdução: A Candida albicans é um dos microorganismos que mais frequentemente colonizam a cavidade bucal de crianças HIV+. Este fungo excreta ácidos, proporcionando uma diminuição do pH em um ambiente já altamente acidificado, como cavidade bucal dessas crianças devido sua dieta hipercalórica, uso de medicamentos açucarados e higiene oral deficiente. Considerando a elevada freqüência de restaurações dentárias em função da alta prevalência de cárie, todos esses fatores, incluindo o metabolismo da C. albicans, podem provocar alterações na superfície de materiais restauradores usados nesses pacientes. Objetivo : O objetivo do estudo foi avaliar, in vitro, a ação da C. albicans, isolada de uma criança HIV+, sobre a superfície de materiais restauradores utlizados na prática odontopediátrica. Material e método : Confeccionou-se 44 blocos de diferentes materiais (2 resinas, 1 compômero e 1 cimento ionomérico de Vidro) separados em 4 grupos (n=11) Todos os blocos foram submetidos a microdureza superfícial inicial (MDI). Posteriormente, foram expostos ao biofilme de C. Albicans formado a partir de 1mL de uma suspensão padronizada contendo 10 5 cels/mL, durante 07 dias. Após, os blocos foram limpos e mantidos sob refrigeração (4ºC) e submetidos à mensuração da microdureza final (MDF). Foram utilizados o Teste de Mann-Whitney para comparação intra grupo entre os valores de MDI e MDF; os valores de perda percentual de microdureza (%PMD) foram comparados com o Teste de Kruskall-Wallis (95% IC). Resultados : Os valores de MDI variaram de 63,54±11,41 a 77,92±10,91, sem diferença entre os grupos (p=0,076). Após exposição ao biofilme, não foram observadas variações significativas na microdureza (MDI X MDF) exceto para o grupo 3 (compômero Vitremer TM ), cujo valor de MDF foi 40,45±7,57 (p=0,001) . O %PMD do compômero (grupo 3) foi significativamente maior (41,16%) que o dos outros materiais (5,35% grupo 1; 7,02% grupo 2; e 9,57% grupo 3) (p=0,036. Conclusão: Conclui-se que a C. Albicans isolada do biofilme dental de criança HIV+ pode causar, in vitro, diminuição significante na microdureza superficial do compômero em comparação aos demais.


Subject(s)
Pediatric Dentistry , Candida albicans , Child , HIV , Dental Plaque , Mouth
5.
Rev. Cient. CRO-RJ (Online) ; 3(2): 42-46, May-Aug. 2018.
Article in English | LILACS, BBO | ID: biblio-1021868

ABSTRACT

Introduction: Linear gingival erythema (LGE), formally referred to as HIV-gingivitis, is the most common form of HIV-associated periodontal disease in HIV-infected individuals These lesions have been recently evaluated as a possible form of erythematous oral candidosis, mainly caused by Candida albicans. Other species such as C. tropicalis, C. stellatoidea, C. krusei, C. parapsilosis, C. glabrata and C. Dubliniensis, have also been identified in some HIV-infected subjects. Objective: This case report reveals the presence of typical LGE lesions in six HIV-infected children, and also investigates the etiologic agent through microbiological exams and correlates this oral manifestation with the patients' systemic conditions. Case report: Microbiological analyses showed growth for Candida spp in all patients; all of whom had severe immunosuppression. However, the regression of lesions was noted after antifungal medication. Conclusion: The presence of LGE in pediatric patients with AIDS may be a predictive marker in the progression of AIDS or it may be the first clinical manifestation of HIV infection in children. Hence it is important for dentists to be aware of such lesions.


Introdução: O eritema gengival linear (LGE), normalmente referido como gengivite do HIV, é a forma mais comum de doença periodontal presente em indivíduos infectados pelo HIV. Recentemente, estas lesões foram consideradas como uma possível forma de candidíase oral eritematosa causada por Candida. Albicans . Outras espécies, como C. tropicalis, C. stellatoidea,C. krusei, C. parapsilosis, C. glabrata e C. Dubliniensis também foram identificadas em indivíduos infectados pelo HIV associadas ao LGE. Objetivo: O presente artigo mostra a presença de lesões típicas de LGE em seis crianças infectadas pelo HIV e também investigou o agente etiológico das lesões orais através de exames microbiológicos, correlacionando o LGE com as condições sistêmicas dos pacientes. Case report: Análises microbiológicas mostraram crescimento positivo para Candida spp em todos os pacientes, os quais possuiam imunossupressão grave. Adicionalmente, a regressão total das lesões foi observada após medicação tópica antifúngica. Conclusão: A presença de LGE em pacientes pediátricos com AIDS pode ser um marcador preditivo da progressão.


Subject(s)
Periodontics , Oral Manifestations , Periodontal Diseases , Candida albicans , Child , HIV
6.
J. appl. oral sci ; 26: e20170270, 2018. tab, graf
Article in English | LILACS, BBO | ID: biblio-893712

ABSTRACT

Abstract Although resin composites are widely used in the clinical practice, the development of recurrent caries at composite-tooth interface still remains as one of the principal shortcomings to be overcome in this field. Objectives To evaluate the activity against S. mutans biofilm of model resin composites incorporating different concentrations of ZnO-nanoparticles (ZnO-NP) and characterize their physicochemical properties. Materials and Methods Different concentrations of ZnO-NP (wt.%): E1=0, E2=0.5, E3=1, E4=2, E5=5 and E6=10 were incorporated into a model resin composite consisting of Bis-GMA-TEGDMA and barium borosilicate particles. The activity against S. mutans biofilm was evaluated by metabolic activity and lactic acid production. The following physicochemical properties were characterized: degree of conversion (DC%), flexural strength (FS), elastic modulus (EM), hardness (KHN), water sorption (Wsp), water solubility (Wsl) and translucency (TP). Results E3, E4, E5 and E6 decreased the biofilm metabolic activity and E5 and E6 decreased the lactic acid production (p<0.05). E6 presented the lowest DC% (p<0.05). No significant difference in FS and EM was found for all resin composites (p>0.05). E5 and E6 presented the lowest values of KHN (p<0.05). E6 presented a higher Wsp than E1 (p<0.05) and the highest Wsl (p<0.05). The translucency significantly decreased as the ZnO- NP concentration increased (p<0.05). Conclusions The incorporation of 2 - 5 wt.% of ZnO-NP could endow antibacterial activity to resin composites, without jeopardizing their physicochemical properties.


Subject(s)
Streptococcus mutans/drug effects , Zinc Oxide/pharmacology , Zinc Oxide/chemistry , Composite Resins/pharmacology , Composite Resins/chemistry , Nanoparticles/chemistry , Polyethylene Glycols/pharmacology , Polyethylene Glycols/chemistry , Polymethacrylic Acids/pharmacology , Polymethacrylic Acids/chemistry , Reference Values , Solubility , Streptococcus mutans/growth & development , Surface Properties , Materials Testing , Water/chemistry , Microscopy, Electron, Scanning , Linear Models , Reproducibility of Results , Analysis of Variance , Bisphenol A-Glycidyl Methacrylate/pharmacology , Bisphenol A-Glycidyl Methacrylate/chemistry , Silicates/pharmacology , Silicates/chemistry , Barium Compounds/pharmacology , Barium Compounds/chemistry , Pliability , Biofilms/growth & development , Biofilms/drug effects , Elastic Modulus , Hardness Tests
7.
J. appl. oral sci ; 25(1): 53-60, Jan.-Feb. 2017. tab, graf
Article in English | LILACS, BBO | ID: biblio-841164

ABSTRACT

Abstract Objectives This study analyzed the capacity of Candida spp. from dental biofilm of HIV infected (HIV+) children to demineralize primary molar enamel in vitro by Transversal Microhardness (TMH), Polarized Light Microscopy (PLM) and the quantity of calcium ions (Ca2+) released from the enamel. Material and Methods Candida spp. samples were isolated from the supragingival biofilm of HIV+ children. A hundred and forty (140) enamel blocks were randomly assigned to six groups: biofilm formed by C. albicans (Group 1); mixed biofilm formed by C. albicans and C. tropicalis (Group 2); mixed biofilm formed by C. albicans and C. parapsilosis (Group 3); mixed biofilm formed by C. albicans, C. parapsilosis and C. glabrata (Group 4); biofilm formed by C. albicans ATCC (Group 5) and medium without Candida (Group 6). Enamel blocks from each group were removed on days 3, 5, 8 and 15 after biofilm formation to evaluate the TMH and images of enamel were analyzed by PLM. The quantity of Ca2+ released, from Groups 1 and 6, was determined using an Atomic Absorption Spectrophotometer. The SPSS program was used for statistical analysis and the significance level was 5%. Results TMH showed a gradual reduction in enamel hardness (p<0.05) from the 1st to 15th day, but mainly five days after biofilm formation in all groups. The PLM showed superficial lesions indicating an increase in porosity. C. albicans caused the release of Ca2+ into suspension during biofilm formation. Conclusion Candida species from dental biofilm of HIV+ children can cause demineralization of primary enamel in vitro.


Subject(s)
Humans , Male , Female , Child, Preschool , Child , Candida/isolation & purification , Candida/pathogenicity , HIV Infections/microbiology , Dental Caries/microbiology , Dental Enamel/microbiology , Reference Values , Spectrophotometry, Atomic , Time Factors , Tooth, Deciduous/microbiology , Tooth, Deciduous/virology , Virulence , In Vitro Techniques , Candida/growth & development , Candida/virology , HIV Infections/complications , Calcium/metabolism , Analysis of Variance , Biofilms/growth & development , Dental Caries/virology , Dental Enamel/virology , Dental Plaque/microbiology , Dental Plaque/virology , Hardness Tests , Microscopy, Polarization
8.
Braz. oral res. (Online) ; 31: e84, 2017. tab, graf
Article in English | LILACS | ID: biblio-952087

ABSTRACT

Abstract: The study aimed to investigate the effects of bacterial biofilms on changes in the surface microhardness of enamel treated with casein phosphopeptide—amorphous calcium phosphate (CPP-ACP) with and without fluoride. Human enamel blocks with incipient caries-like lesions were divided into four groups of 13: G1: Saliva (Control); G2: fluoride dentifrice (Crest™, 1100 ppm as NaF); G3: CPP-ACP (MI Paste; Recaldent™); and G4: CPP-ACPF (MI Paste Plus; Recaldent™ 900 ppm as NaF). The specimens were soaked in demineralizing solution for 6 h and remineralized in artificial saliva for 18 h alternately for 10 days. The dentifrice was prepared with deionized water in a 1 : 3 ratio (w/w) or applied undiluted in the case of the CPP-ACP group. The surface microhardness (SMH) was evaluated at baseline, after artificial caries, after pH cycling and treatment with dentifrices, and after incubation in media with Streptococcus mutans for biofilm formation. The biofilms were exposed once a day to 2% sucrose and the biofilm viability was measured by MTT reduction. The percentage of change in surface microhardness (%SMHC) was calculated for each block. The data were analyzed by nonparametric test comparisons (α = 0.05). The %SMHC values observed in G2 were different from those of G1, G3, and G4 (p < 0.05). After biofilm formation, %SMHC was positive in G2 and G4 when compared to G1 and G3, but resistance to demineralization after biofilm formation was similar in all groups. In conclusion, the presence of biofilms did not influence the treatment outcomes of anticaries products.


Subject(s)
Humans , Streptococcus mutans/physiology , Cariostatic Agents/chemistry , Caseins/chemistry , Biofilms/growth & development , Dental Enamel/drug effects , Dental Enamel/microbiology , Fluorides/chemistry , Reference Values , Saliva, Artificial/chemistry , Streptococcus mutans/drug effects , Surface Properties , Time Factors , Tooth Remineralization/methods , Materials Testing , Reproducibility of Results , Statistics, Nonparametric , Biofilms/drug effects , Dentifrices/chemistry , Hardness Tests
9.
Odontol. clín.-cient ; 10(3): 297-300, Jul.-Set. 2011. ilus
Article in Portuguese | LILACS-Express | LILACS | ID: lil-725286

ABSTRACT

O objetivo deste trabalho é o de relatar um caso de reabilitação estético-funcional por meio de pino e faceta biológica de um incisivo central decíduo. Paciente, com 4 anos de idade, atendido na clínica de Odontopediatria, com queixa principal estética do elemento 51, o qual apresentava perda de estrutura dentária, descoloração coronária e tratamento endodôntico prévio. O tratamento consistiu na confecção de um pino e faceta biológica e no acompanhamento clínico e radiográfico durante 1 ano. O uso da restauração biológica, com coroa e raiz naturais, neste caso, resultou em sucesso clínico, recuperando a função e a estética. A técnica biológica é uma alternativa promissória para restaurações protéticas em dentes decíduos com grande perda de estrutura.


The objective of this study was to present a case report of aesthetic and functional rehabilitation using biological post and facet in a devitalized primary central incisor. Patient, 4 years old, attended at Pediatric Dentistry clinic and the main complain was the aesthetic of element 51. It presented loss of tooth structure, crown discoloration and previous endodontic treatment. The treatment consisted of restoration with a biological post and facet. The tooth is being one year clinic and radiographically followed up. The use of biological restoration, with biological crown and root, in this case, resulted in clinical success recovering function and aesthetics. A biological technique is a promising alternative for prosthetic restorations in primary teeth with great loss of structure.

10.
ImplantNews ; 8(3): 307-312, 2011.
Article in Portuguese | LILACS, BBO | ID: lil-599222

ABSTRACT

Uma conexão estável entre a superfície de titânio do implante e os tecidos bucais a sua volta é um importante pré-requisito para o seu sucesso a longo prazo. Assim, tal superfície deve possuir propriedades que minimizem a colonização por microrganismos patogênicos da cavidade oral ao mesmo tempo em que permita boa adesão dos tecidos bucais. O objetivo deste estudo foi realizar uma revisão da literatura que buscasse relacionar as diferentes propriedades de superfícies dos implantes e suas características com as respostas dos tecidos moles peri-implantares, bem como com a formação de biofilme sobre sua superfície. Estudos in vitro e in vivo indicaram que a rugosidade e a textura de superfície do implante influenciam em adesão, orientação, proliferação, metabolismo das células epiteliais e células de tecido conjuntivo. Foi descrito também que a rugosidade superficial e a molhabilidade têm um impacto significativo na formação de biofilme, sendo que a influência da rugosidade foi dita como sendo mais significativa do que a molhabilidade. Sendo assim, nota-se que a otimização das superfícies dos implantes ainda é necessária. Tornando-se importante analisar o papel das propriedades de diferentes superfícies com relação à composição química e à microestrutura, avaliando o desempenho biológico dos diferentes tratamentos de superfícies e as propriedades químicas que eles promovem na superfície dos implantes dentários.


A stable connection between the titanium surface and the oral tissue around them is an important prerequisite for long-term dental implant success. Thus, this surface should minimize the bacterial colonization while allowing a good adhesion of the oral tissues. The aim of this study was to review the literature and to relate the properties of implant surfaces and their characteristics with the responses of oral soft tissues around implants, as well as the biofilm formation on its surface. In vitro and in vivo studies indicated that the roughness and texture of the implant surface influences the adhesion, orientation, proliferation, and metabolism of epithelial cells and connective tissue. It was also described that the surface roughness and wettability, have a significant impact on the biofilm formation, but the influence of roughness was said to be more significant than the wettability. In summary, it appears that an optimization of implant surfaces is still necessary. It is therefore important to systematically evaluate the role of different surface properties (chemical composition as well as microstructure) and to assess the biological performance of different implant materials.


Subject(s)
Dental Plaque , Biocompatible Materials/analysis , Periodontium , Prostheses and Implants
11.
Braz. oral res ; 24(4): 406-412, Oct.-Dec. 2010. graf, tab
Article in English | LILACS | ID: lil-569218

ABSTRACT

The present study evaluated the antimicrobial activity of three root canal irrigants against Enterococcus faecalis, Candida albicans, and Staphylococcus aureus. These microorganisms were incubated in the presence of citric acid (6 and 10 percent), EDTA (17 percent), and NaOCl (0.5, 1.0, 2.5, and 5.25 percent). Agar diffusion tests were performed and redox indicator resazurin was used to evaluate the inhibitory effect of the irrigants on the metabolic activity of these microorganisms. The mean diameters of the inhibition zones for the C. albicans cultures were 11.6 mm (17 percent EDTA), 5.5 mm (0.5 percent NaOCl), 12.9 mm (1 percent NaOCl), 22.1 mm (2.5 percent NaOCl), and 28.5 mm (5.25 percent NaOCl). The mean diameters of the inhibition zones for E. faecalis were 2.8 mm (1 percent NaOCl), 5.4 mm (2.5 percent NaOCl), and 8.3 mm (5.25 percent NaOCl). For S. aureus, the mean values were 8.0 mm (17 percent EDTA), 3.0 mm (1 percent NaOCl), 8.8 mm (2.5 percent NaOCl), and 10.0 mm (5.25 percent NaOCl). Most of the irrigant solutions presented effective antimicrobial activity against C. albicans. A high inhibitory effect on the metabolic activity of E. faecalis was detected when the microorganisms were incubated with 17 percent EDTA. The same result was reached when S. aureus was incubated in the presence of > 2.5 percent NaOCl. Altogether, these results indicate that 2.5 percent and 5.25 percent NaOCl are microbicides against S. aureus while 0.5 percent and 1 percent NaOCl are only microbiostatic against the tested bacteria. The 6 percent and 10 percent citric acid as well as 17 percent EDTA did not affect the viability of any of the assayed microorganisms.


Subject(s)
Candida albicans/drug effects , Enterococcus faecalis/drug effects , Root Canal Irrigants/pharmacology , Staphylococcus aureus/drug effects , Analysis of Variance , Anti-Infective Agents, Local/pharmacology , Citric Acid/pharmacology , Edetic Acid/pharmacology , Microbial Sensitivity Tests , Sodium Hypochlorite/pharmacology , Time Factors
12.
Rev. odontol. UNESP (Online) ; 39(5): 271-275, set.-out. 2010. tab
Article in Portuguese | LILACS, BBO | ID: biblio-874527

ABSTRACT

Candidíase oral é a infecção oportunista mais comum em pacientes imunocomprometidos, sendo a clorexidina um importante antimicrobiano auxiliar no seu tratamento. O objetivo do presente estudo foi avaliar o efeito antifúngico de diferentes soluções de clorexidina (Periogard®, NoPlak Max®, Noplak®, Perioxidin®, Clorexidina 0.06%, Paradontax® e Clorexidina 1%) sobre biofilmes artificiais de Candida spp.: C. albicans (ATCC36801); C. parapsilosis (ATCC22019); C. krusei (ATCC6258); C. glabrata (ATCC2001) e C. tropicalis (ATCC750). As cepas foram cultivadas em meio de cultura BHI ágar sobre fragmentos de esmalte bovino por 72 horas a 37 °C. Após o crescimento, cada fragmento de esmalte bovino foi imerso nas diferentes soluções de Clorexidina por 3 minutos. Nistatina e solução salina foram utilizadas como controle negativo e positivo, respectivamente. Para remoção das células não aderidas, os fragmentos foram então imersos em solução salina por 10 minutos e agitados em vortex. Alíquotas de 100 µL foram inoculadas em placas contendo BHI ágar por 24 horas a 37 °C para contagem de unidades formadoras de colônias (UFC). Observamos que o número de UFC de C. albicans e C. parapsilosis, apresentou um percentual de redução variando de 79 a 99% quando do uso das diferentes soluções (p < 0,001), o mesmo não foi observado para o NoPlak Max® (2,94 e 1,3%, respectivamente); Para C. krusei e C. glabrata, a solução menos efetiva foi a Nistatina (23 e 3,4%, respectivamente) enquanto que para C. tropicalis, todas as soluções apresentaram um alto percentual de redução (99 a 100%). As soluções de clorexidina foram capazes de reduzir significativamente o número de UFC provenientes de biofilme de Candida spp. in vitro.


Oral candidiasis is the most common opportunistic infection in immunocompromised patients and chlorhexidine is an important antimicrobial for its treatment. The antifungal effect of different CHX solutions (Periogard®, NoPlak Max®, Noplak®, Perioxidin®, Chlorhexidine 0.06%, Paradontax® and Chlorhexidine 1%) was evaluated on artificial biofilms of Candida spps: C. albicans (ATCC36801), C. parapsilosis (ATCC22019), C. krusei (ATCC6258), C. glabrata (ATCC2001) and Candida tropicalis (ATCC750). The strains were grown, in a BHI agar medium on bovine teeth enamel for 72 hours at 37 °C. After growth, the fragments were immersed in the CHX solutions for 3 minutes. Nystatin and saline solutions were used as positive and negative controls respectively. To remove the non-adhered cells, the fragments were inoculated in saline solution for 10 minutes, transferred to Falcon tubes containing saline solution and mixed in a vortex. Aliquots of 100 µL were inoculated on BHI agar for 24 hours at 37 °C to count the number of colony forming units (CFU). We observed that the number of (CFU) of C. albicans and C. parapsilosis, showed a reduction rate ranging from 79 to 99% with the use of different solutions (p < 0.001), except for NoPlak Max® (2.94 and 1.3%, respectively). For C. krusei and C. glabrata, nystatin was the least effective solution (23 and 3.4%, respectively); and for C. tropicalis, all the substances presented a high reduction percentage (99-100%). The chlorhexidine solutions were able to reduce the colony forming units of Candida biofilm.


Subject(s)
Biofilms , Candida , Chlorhexidine
13.
Pesqui. bras. odontopediatria clín. integr ; 10(2): 249-255, maio-ago. 2010.
Article in English | LILACS, BBO | ID: lil-568486

ABSTRACT

Objetivo: Analisar in vitro a influencia da presenca de um biofilme de Candida albicans na microdureza superficial de duas marcas de cimentos de ionomero de vidro modificados por resina encontrados no mercado, o Vitremer© e o Vitro Fill© LC. Metodo: Nove amostras de cada material foram confeccionadas utilizando-se moldes de plasticos, previamente padronizados. Os nove especimes de cada marca foram divididos em tresgrupos: G1 solucao salina; G2 BHI liquido sem C. albicans; G3 suspensao celular com 105 de leveduras/mL em BHI liquido e a inducao da formacao de biofilme ocorreu apos incubacao das placas a 37§C por 48 horas. Os especimes foram esterilizados atraves de oxido de etileno. Foi avaliada a microdureza Knoop (50g, 15s). Os resultados foram submeti dos a Analise de Variancia (ANOVA) e teste de Tukey (5%). Resultados: Os grupos G1 e G2 obtiveram valores medios de dureza (KHN) maiores que G3 para ambos os materiais testados. Comparando os grupos G2 e G3, os especimes de G3 apresentaram menor resistencia as indentacoes. Conclusao: Nas condicoes do estudo in vitro, o biofilme de Candida albicans apresentou potencial de reduzir a dureza superficial de dois ionomeros de vidro modificados por resina encontrados no mercado (Vitremer© e Vitro Fill LC©).


Objective: To assess in vitro the influence of the presence of a Candida albicans biofilm on the surface microhardness of two commercial brands of resin-modified glass ionomer cements, Vitremer© and Vitro Fill© LC. Method: Nine samples of each material were fabricated using standardized plastic molds. The nine specimens of each brand were assigned to three groups: G1: saline; G2: BHI broth without C. albicans; G3: cell suspension with 105 yeasts/mL in BHI broth. Induction of biofilm formation occurred after incubation of the plates at 37§C for 48 hours and the specimens were sterilized with ethylene oxide. Knoop microhardness was measured (50 g, 15 s). Data were subjected to ANOVA and Tukey test at 5% significance level. Results: G1 and G2 presented higher mean hardness values (KHN) than G3 for both tested materials. Comparing G2 and G3, G3?s specimens presented lower resistance to theindentations. Conclusion: Under the conditions of this in vitro study, the Candida albicans biofilm reduced the surface hardness of two commercially available resin-modified glass ionomer cements (Vitremer© and Vitro Fill LC©).


Subject(s)
Biofilms , Candida albicans , Glass Ionomer Cements , In Vitro Techniques , Materials Testing , Analysis of Variance , Statistics, Nonparametric
14.
Rev. bras. odontol ; 59(5): 341-343, set.-out. 2002. tab, graf
Article in Portuguese | LILACS, BBO | ID: lil-331290

ABSTRACT

Foram determinadas as frequências de candidíase bucal, graus de imunossupressäo e uso de anti-retrovirais em crianças infectadas pelo HIV, de 1997 a 2000. Prontuários de 18 crianças, com idade entre 07 e 12 anos, foram pesquisados, observando-se reduçäo da frequência de candidíase bucal ao longo dos anos, aumento do uso de terapia anti-retroviral combinada e melhora na condiçäo imunológica, embora sem significância estatística . Verificou-se correlação significante entre ocorrência de candidíase e imunossupresssäo grave (p=0,0048). Os resultados sugerem que a terapia combinada pode contribuir para diminuir a frequência de candidíase bucal em pacientes infectados pelo HIV


Subject(s)
Humans , Male , Female , Child , Candidiasis, Oral/epidemiology , Candidiasis, Oral/etiology , Child , Combined Modality Therapy , HIV Seropositivity , Immunosuppression Therapy , AIDS-Related Opportunistic Infections/etiology , Prevalence , Protease Inhibitors , Retrospective Studies
15.
Rev. bras. odontol ; 59(1): 19-21, jan.-fev. 2002. tab, graf
Article in Portuguese | LILACS, BBO | ID: lil-312584

ABSTRACT

O objetivo foi verificar o conhecimento e atitudes de todos os médicos do Ambulatório da AIDS Pediátrica/IPPMG (n=12) que atendem as crianças HIV+ através de um questionário sobre cárie de estabelecimento precoce (CP). Todos (100 por cento) sabiam o que era CP, mas 50 por cento consideraram maior o risco de uma criança HIV+ apresentar a doença. Todos acham que o médico tem responsabilidade na prevençäo da CP, mas 41,7 por cento atuam diretamente nesta prática. Campanhas sobre saúde bucal devem ser realizadas junto aos profissionais que lidam com estes pacientes, já que säo mais sujeitos a infecçöes bucais


Subject(s)
Humans , Adult , Male , Female , Dental Caries/etiology , Clinical Competence , Health Knowledge, Attitudes, Practice , Opportunistic Infections/etiology , Oral Hygiene , Professional Practice/trends , Risk Factors , Acquired Immunodeficiency Syndrome/complications
16.
Rev. paul. pediatr ; 19(4): 202-206, dez. 2001. ilus
Article in Portuguese | LILACS | ID: lil-363093

ABSTRACT

O presente artigo objetiva relatar um caso de dois dentes natais em um bebê de 7 meses de idade do sexo masculino. Ressalta-se também o papel desses elementos dentários como fator de risco para a cárie, o tratamento realizado, as condutas preventivas necessárias, bem como a importância do encaminhamento precoce para o odontopediatra. Os elementos irrompidos constituíram-se dos incisivos centrais inferiores decíduos e apresentavam exposição pulpar resultante da atividade de cárie decorrente da ausência de higiene bucal e presença de mamadeira noturna, com adição de açúcar, associada a refluxo gastroesofágico.


Subject(s)
Humans , Male , Infant , Dental Caries Susceptibility , Natal Teeth
17.
Pesqui. odontol. bras ; 15(2): 91-7, abr.-jun. 2001. tab, graf
Article in Portuguese | LILACS, BBO | ID: lil-298520

ABSTRACT

Este estudo tem como objetivo determinar a experiência de cárie em crianças infectadas pelo HIV, correlacionada com a classificaçäo clínica e imunológica (CDCü, 1994). Participaram da pesquisa 92 crianças (50 meninas e 42 meninos), com média de idade de 5,77 ñ 3,31 anos (6 meses a 13 anos), com diagnóstico definitivo para o HIV, todos pacientes com atendimento ambulatorial. Foi realizado exame bucal para determinar os índices ceo/CPOD e os dados de história médica foram coletados dos prontuários médicos. Na dentiçäo decídua de crianças mais jovens (até 5 anos), apesar de a prevalência de cárie ter apresentado valores maiores naquelas mais comprometidas clínica e imunologicamente, näo houve diferença estatisticamente significante entre as diversas categorias de classificaçäo. Nas crianças de maior idade, isto também foi observado, apesar de terem sido encontrados valores de CPOD maiores nas crianças mais doentes


Subject(s)
Humans , Male , Female , Child , Dental Caries/epidemiology , HIV Infections
18.
Rev. bras. odontol ; 58(2): 88-91, mar.-abr. 2001. ilus, tab, graf
Article in Portuguese | LILACS, BBO | ID: lil-296626

ABSTRACT

Alteraçöes gengivais säo encontradas em crianças infectadas pelo HIV. O objetivo deste estudo foi determinar a condiçäo de saúde gengival e 35 crianças com diagnóstico definitivo para infecçäo pelo HIV e sua relaçäo com o número de leucócitos (L) e grau de imunossupressäo (por centoCD4). A frequência de crianças com alteraçäo gengival foi de 80 por cento. Três crianças (8,6 por cento) apresentavam Eritema Linear Gengival e, dessas, todas tinham imunossupressäo grave e leucopenia. Näo houve diferença estatisticamente significativa entre as crianças com e sem alteraçäo gengival, mas a frequência de pacientes com leucopenia e imunossupressäo grave foi maior naquelas crianças com alguma alteraçäo gengival, principalmente quando esta era mais severa


Subject(s)
Humans , Child, Preschool , Gingivitis/classification , Gingivitis/diagnosis , Gingivitis/epidemiology , Gingivitis/etiology , Oral Health , Prevalence , Acquired Immunodeficiency Syndrome/complications , Leukopenia/complications
19.
JBP, j. bras. odontopediatr. odontol. bebê ; 2(5): 14-18, jan.-fev. 1999. ilus, graf
Article in Portuguese | LILACS, BBO | ID: lil-405770

ABSTRACT

O presente artigo apresenta um Programa de Atendimento Primário Odontológico para bebês soropositivos para o HIV, realizado pelo Projeto AIDS em Odontopediatria (Disciplina de Odontopediatria: FO / NESC / IPPMG- UFRJ), bem como relata as principais manifestações encontradas nessas crianças


Subject(s)
Humans , Male , Female , Infant , Dental Care for Children , HIV Seropositivity , Mouth Diseases , Dental Caries , Health Programs and Plans , Tooth Eruption
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