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1.
Article in Chinese | WPRIM | ID: wpr-906428

ABSTRACT

Objective:To observe the effects of modified Huangqi Biejiatang combined with auricular acupressure on diabetic peripheral neuropathy (DPN) due to Qi and Yin deficiency and serum myeloid differentiation factor 88/inhibitor of nuclear factor-<italic>κ</italic>B (MyD88/I<italic>κ</italic>B) signaling pathway. Method:One hundred and forty cases were randomly divided into an observation group (<italic>n</italic>=70) and a control group (<italic>n</italic>=70). In addition to routine treatments like dietary intervention and the regulation of fasting blood glucose (FBG) and blood pressure, the modified Huangqi Biejiatang combined with auricular acupressure was further provided in the observation group, while mecobalamine was administered in the control group. After four-week intervention, the toronto clinical scoring system (TCSS) score, traditional Chinese medicine (TCM) syndrome score, the conduction velocities of motor and sensory nerves (median nerve, common peroneal nerve, tibial nerve, and ulnar nerve), glucose metabolism indexes [fasting plasma glucose (FPG), 2 h postprandial blood glucose (2 h PG), and hemoglobin A1c (HbA1c)], intestinal genera (<italic>Clostridium</italic>, <italic>Prauserella</italic>, <italic>Bacteroides</italic>, and <italic>Faecalibacterium</italic>), as well as the serum MyD88, I<italic>κ</italic>B<italic>α</italic>, and phosphorylated I<italic>κ</italic>B<italic>α </italic>(p-I<italic>κ</italic>B<italic>α</italic>) levels in the MyD88/I<italic>κ</italic>B signaling pathway before and after treatment were observed in the two groups, for comparing their clinical efficacy and safety. Result:The total effective rate of the observation group was 85.3% (58/68), which was higher than 48.5% (32/66) of the control group (<italic>χ</italic><sup>2</sup>=6.143, <italic>P</italic><0.05). The comparison with the control group revealed that the scores of TCSS and TCM syndrome, the levels of FPG, 2 h PG, HbA1c, MyD88, and p-I<italic>κ</italic>B<italic>α</italic>, as well as the abundances of <italic>Clostridium</italic> and <italic>Prauserella</italic> in the observation group were decreased (<italic>P</italic><0.05), while the conduction velocities of motor and sensory nerves (median nerve, common peroneal nerve, tibial nerve, and ulnar nerve) were significantly accelerated (<italic>P</italic><0.05). Besides, the abundances of <italic>Bacteroides</italic> and <italic>Faecalibacterium</italic> and I<italic>κ</italic>B<italic>α</italic> level were significantly elevated (<italic>P</italic><0.05). The incidence of adverse reactions in the observation group was 1.5% (1/68), lower than 12.1% (8/66) in the control group (<italic>χ</italic><sup>2</sup>=4.328, <italic>P</italic><0.05). Conclusion:The modified Huangqi Biejiatang combined with auricular acupressure alleviates DPN due to Qi and Yin deficiency, which may be attributed to the regulation of serum MyD88/I<italic>κ</italic>B signaling pathway.

2.
Article in Chinese | WPRIM | ID: wpr-906307

ABSTRACT

Objective:To establish a new fast and accurate method for identifying the authenticity and specifications of Fritillariae Cirrhosae Bulbus based on electronic nose technology, and to discuss the feasibility of this technology in the identification of decoction pieces. Method:Fritillariae Cirrhosae Bulbus was used as the research object, 80 batches of samples to be tested were collected, and the olfactory sensory data of the electronic nose were taken as independent variables (<italic>X</italic>), the results of the method contained in the 2020 edition of <italic>Chinese Pharmacopoeia</italic> were taken as the focus, and the traditional empirical identification results were used as benchmarking information (<italic>Y</italic>). Four chemometric methods, including discriminant analysis (DA), least square support vector machine (LS-SVM), principal component analysis-DA (PCA-DA) and partial least squares-DA (PLS-DA), were used to establish the identification model [<italic>Y</italic>=<italic>F</italic>(<italic>X</italic>)] of authenticity and commodity specifications of Fritillariae Cirrhosae Bulbus, respectively. Wherein, the identification accuracy and time-consuming was taken as indicators to discuss the results. Result:After cross-verification by leave-one-out method, the correct rates of the above four models were 93.75%, 91.25%, 95.00% and 95.00%, respectively, and the PCA-DA and PLS-DA identification models were the best in terms of authenticity identification. In specification identification, the correct rates of these four models were 86.67%, 88.00%, 89.33% and 68.00%, respectively, and the PCA-DA identification model was the best. The electronic nose had a high accuracy in the identification of authenticity and specification model, and the time consuming was relatively short. Conclusion:Electronic nose technology can identify Fritillariae Cirrhosae Bulbus accurately and quickly, and has significant advantages in terms of timeliness and correct judgment rate.

3.
Article in Chinese | WPRIM | ID: wpr-906022

ABSTRACT

Objective:To observe the therapeutic effect of modified Fuyuanwan combined with auricular acupressure bean on stage Ⅱ, Ⅲ diabetic nephropathy and its effect on serum janus kinase (JAK)/ signal transducer and activator of tranions (STAT) signaling pathway. Method:A total of 180 cases were randomly divided into control group and observation group, 90 cases in each group. Losartan potassium, modified Fuyuanwan combined with auricular acupressure bean were given respectively for 12 weeks. Renal function indexes [blood urea nitrogen (BUN), serum creatinine (SCr), urinary albumin excretion rate (UAER), 24 h urinary protein quantitative (24 h Upor)], relative abundance of intestinal flora (verruca microflora, scleriobacteriae, deferribacter, proteobacteria), oxidative stress indicators [advanced oxidation protein products (AOPPs), reactive oxygen species (ROS), glutathione peroxidase (GSH-Px), total superoxide dismutase (TSOD)], renal blood flow index [end-diastolic blood flow velocity (EDV), peak systolic value (PSV), pulse index (PI), blood flow resistance index (RI)], JAK/STAT signaling pathway [JAK, phosphorylated JAK (p-JAK), STAT, phosphorylated STAT (p-STAT) were observed before and after treatment. The safety indexes of two groups were evaluated after treatment. The efficacy was observed after treatment and followed up for 1 years and 2 years. Result:After treatment and follow-up for 1, 2 years, the total effective rates of patients in observation group were 97.8% (87/89), 81.6% (71/87), 59.8% (49/82), respectively, observation group which were significantly higher than those in control group of 79.3%(69/87),57.8%(48/83),37.2%(29/78) (<italic>χ</italic><sup>2</sup>=4.016, <italic>χ</italic><sup>2</sup>=4.503, <italic>χ</italic><sup>2</sup>=4.769, <italic>P</italic><0.05). Compared with control group after treatment, UAER, BUN, SCr, 24 h Upor, firmicutes, actinobacillus, proteobacteria, AOPPs, ROS, PI, RI, p-JAK, p-STAT3 in observation group were significantly decreased (<italic>P</italic><0.05,<italic>P</italic><0.01), microflora verruca, GSH-PX, TSOD, JAK, STAT3 were significantly increased (<italic>P</italic><0.05,<italic>P</italic><0.01), EDV and PSV were significantly accelerated (<italic>P</italic><0.05,<italic>P</italic><0.01). The incidence of adverse reactions was 1.1% (1/89) in observation group, lower than 13.8% (12/87) in control group (<italic>χ</italic><sup>2</sup>=5.127, <italic>P</italic><0.05). Conclusion:Modified Fuyuanwan combined with auricular acupressure bean can significantly improve the curative effect of stage Ⅱ, Ⅲ diabetic nephropathy, and its mechanism of action may be related to the serum JAK/STAT signaling pathway.

4.
Article in Chinese | WPRIM | ID: wpr-905995

ABSTRACT

Objective:To observe the clinical efficacy of modified Huqianwan combined with auricular acupressure with seeds on girls with central precocious puberty (CPP) due to Yin deficiency and fire hyperactivity. Method:A total of 150 cases were randomly divided into three groups (A group,B group, and C group),with 50 cases in each group. The cases received modified Huqianwan,Zhibo Dihuangwan,and modified Huqianwan combined with auricular acupressure with seeds, respectively, for 12 weeks. The traditional Chinese medicine (TCM) symptoms,bone age difference/chronological age difference (ΔBA/ΔCA), predicted adult height (PAH),ovarian volume,uterine volume,breast nodule index,serum sex hormones [follicle-stimulating hormone (FSH),luteinizing hormone (LH),and estradiol (E<sub>2</sub>)],and serum kisspeptin-1 (KISS-1)/G protein-coupled receptor 54 (GPR54)/hypothalamic gonadotropin-releasing hormone (GnRH) signaling pathway of the three groups were observed before and after treatment. The clinical efficacy and safety of the three groups were evaluated. Result:The total effective rate was 97.9% in the C group, which was higher than 81.3% in the A group (<italic>χ</italic><sup>2</sup>=4.516,<italic>P</italic><0.05) and 71.4% in the B group (<italic>χ</italic><sup>2</sup>=5.162,<italic>P</italic><0.05). Compared with the results in the A group after treatment,TCM syndrome,ΔBA/ΔCA,E<sub>2</sub>,LH,KISS-1,GPR54,and GnRH decreased(<italic>P</italic><0.05),and ovarian volume,uterine volume,and breast nodule index were reduced (<italic>P</italic><0.05),but PAH increased in the C group (<italic>P</italic><0.05). Compared with the B group after treatment,the C group showed decreased TCM syndrome,ΔBA/ΔCA, FSH,E<sub>2</sub>,LH,KISS-1,GPR54,and GnRH (<italic>P</italic><0.05),reduced ovarian volume,uterine volume,and breast nodule index (<italic>P</italic><0.05),and increased PAH(<italic>P</italic><0.05). During the study,there was one case of anorexia in the A group,and no obvious adverse reactions were found in the B group and the C group. Conclusion:Modified Huqianwan combined with auricular acupressure with seeds can significantly increase the PAH of girls with CPP due to Yin deficiency and fire hyperactivity and improve the serum sex hormones and the KISS-1/GPR54/GnRH signaling pathway.

5.
Article in Chinese | WPRIM | ID: wpr-905261

ABSTRACT

Objective:To investigate the effect of individualized memory rehabilitation based on the forgetting characteristics in patients with brain injury (BI). Methods:From September, 2018 to October, 2019, 60 BI patients were randomly divided into routine memory training group (control group, n = 30) and individualized memory training group (observation group, n = 30). The observation group group was tested with forgetting characteristics pre-training. Both groups received memory training for four weeks, respectively. All patients were evaluated with Rivermead Behavioural Memory Test-2nd Edition (RBMT-II) and Montreal Cognitive Assessment (MoCA) pre- and post-training. Results:The total post-training scores of RBMT-II and MoCA were higher than the pre-training scores in both groups (|t| > 3.885, P < 0.01). There was no significant difference in the total scores between two groups pre- and post-training (P > 0.05). The differences between post- and pre-training total scores of RBMT-II and MoCA were significantly higher in the observation group group than in the control group (|Z| > 3.757, P < 0.001). Conclusion:Memory rehabilitation training could improve memory abilities and general cognitive function of BI patients, and it is more effective to carry out individualized memory rehabilitation training based on the forgetting characteristics.

6.
Chinese Journal of Urology ; (12): 830-834, 2020.
Article in Chinese | WPRIM | ID: wpr-869767

ABSTRACT

Objective:To investigate the perioperative and oncological outcomes in patients undergoing laparoscopic radical cystectomy with intracorporealorthotopic neobladder reconstruction.Methods:Clinical data of bladder cancer patients undergoing laparoscopic radical cystectomy(LRC) or robot-assisted radical cystectomy(RARC) with intracorporealorthotopic neobladder reconstruction in Zhejiang Provincial People's Hospital from March 2010 to December 2019 were retrospectively analyzed. There were 166 males and 52 females. The median age was 62 (52, 70) years old. The ASA score was 1-2 in 183 cases (83.9%) and 3 in 35 cases (16.1%). There were 61 cases of hypertension, 28 cases of diabetes, 26 cases of heart disease, 33 cases of history of abdominal surgery, and 3 cases received neoadjuvant chemotherapy. LRC was performed in 82 cases and RARC in 136 cases. The operation was performed by dissecting pelvic lymph nodes from right to left and then cystoprostatectomy. The periureteral blood supply and periperitoneal peritoneum were preserved, and the prostate was resected by intrafascial resection. The 30 cm terminal ileum was used to make a U-shaped new bladder, and then the urethral stump and both sides of the ureter were anastomosed on new bladder without tension. During the operation, two single J tubes were used as ureteral stent.The perioperative and pathological results results were evaluated.Results:The operations of 218 patients were completed successfully and there was no conversion to open operation. The median operation time was 281 (229, 400) ml. Intraoperative blood transfusion was performed in 24 cases (11.0%). Hospital stay was 15 (13, 22) days.Intraoperative complications happened in 11 cases (5.0%). Exhaust time was 2 (1, 3) days. Solid food intake time was 4 (3, 5) days. Total complications within 30 days after operation were 61 cases (28.0%), and total complications within 30-90 days after operation were 81 cases (37.2%). The number of median lymph node dissection in all patients was 19 (14, 24). Positive lymph nodes in 21 cases (9.6%). Positive margin in 6 cases (2.7%). Postoperative tumor pathological stages were T a/T 1/T is stage 48 cases, T 2 stage 134 cases, T 3 stage 36 cases. The median follow-up time of all patients was 33 (20.6, 48.2) months. There were 77 cases of tumor recurrence and 55 cases of death, of which 39 cases were tumor-specific death. The 5-year disease free survival, overall survival , and cancer-specific survival of all patients were 55.4%, 62.4% and 66.4%, respectively. Conclusions:Laparoscopic radical cystectomy and intracorporealorthotopic new bladder reconstruction can be well used in the treatment of muscle invasive or high-risk non-muscle invasive bladder cancer.Laparoscopic intracorporeal bladder reconstruction is feasible with few postoperative complications.

7.
Article in Chinese | WPRIM | ID: wpr-868845

ABSTRACT

Objective:To study the timing of radiofrequency ablation (RFA) in treatment of complicated pyogenic liver abscesses (PLA).Methods:A retrospective analysis was performed on 49 patients with complicated PLA who were treated with the RFA-based treatment modality from August 2010 to January 2020 at Beijing Chaoyang Hospital, West Campus, Capital Medical University, Institute of Hepatobiliary, Pancreas and Spleen Surgery and the Second Hospital, Binzhou. The patients were divided into the early RFA group (≤ 72 h, n=27) and the delayed RFA group (>72 h, n=22) according to the timing of RFA. RFA was guided by laparoscopy combined with Ultrasound or CT. The safety and effective rates of RFA, and the total expenses were evaluated. Results:All patients in both the early and the delayed RFA groups were successfully cured (100%). No serious complications, including biliary leakage and massive hemorrhage, happened in the 2 groups. Significantly longer operating time and hospital stays [(8.1±1.6)d vs. (9.5±1.5)d], and higher hospital costs [(3.4±0.2) ten thousand yuan vs. (3.8±0.4) ten thousand yuan] were found in the delayed RFA group when compared with the early RFA group ( P<0.05). Conclusion:RFA treatment of complicated PLA should be completed within 72 hours of onset of PLA.

8.
Journal of Chinese Physician ; (12): 258-262, 2020.
Article in Chinese | WPRIM | ID: wpr-867237

ABSTRACT

Objective To analyze the clinical efficacy,safety and influencing factors of decitabine (DAC)-based regimens in patients with myelodysplastic syndrome-refractory anemia with excess blasts (MDS-RAEB).Methods We performed a retrospective analysis of 63 patients with MDS-RAEB treated with DAC,evaluated the clinical efficacy and adverse reactions,and analyzed the influencing factors affecting survival.Results Among 63 patients,23 were RAEB-1 and 40 were RAEB-2.The median treatment was 4 (2-13) courses.The total effective rate of DAC for MDS-RAEB was 58.7% (37/63),and the complete response rate was 20.6% (13/63).Among 37 patients who were effective,20 (54.1%) patients performed efficacy after 2 courses.The median course of treatment to achieve the best effect was 3.5 (3-4) courses.With a median follow-up of 14 (2-68) months,63 patients had a overall survival rate (OS) of 84.2% and a 1-year progression-free survival rate (PFS) of 73%.In univariate analysis,the factors that prolonged OS were that the best effect after medication was stable disease (SD) (to achieve complete remission,partial remission,complete bone marrow remission,hematological improvement) (P =0.009) and no thrombocytopenia at first diagnosis (P =0.019),the factor that prolongs PFS is the best effect above SD (P =0.003).Multivariate analysis suggested that the factors affecting OS and PFS were the best curative effects above SD (P =0.015 vs P =0.008).The adverse effects of decitabine in the treatment of MDSRAEB were mainly bone marrow suppression and pulmonary infection.Conclusions Decitabine is an effective and well-tolerated drug in the treatment of MDS-RAEB.Response to decitabine treatment is one of the independent factors affecting the prognosis.

9.
Article in Chinese | WPRIM | ID: wpr-829068

ABSTRACT

OBJECTIVE@#To observe the effect of electroacupuncture (EA) of " acupoint combination" on appetite, body fat, insulin sensitivity and central sensitivity of cholecystokinin (CCK) in obese rats with insulin resistance (IR), and to explore the mechanism of EA on improving obesity with insulin resistance.@*METHODS@#Among the fifty 8-week-old healthy SPF male Wistar rats, 10 rats were randomly selected and fed with normal diet; after 8 weeks, 8 rats were randomly selected as a normal group. The remaining 40 rats were fed with high-fat diet to establish the model of obsesity IR; after 8 weeks, 24 rats with successful model of obsesity IR were randomly divided into a model group, an EA group and a sham EA group, 8 rats in each group. Eight weeks after model establishment, the rats in the EA group were intervened with EA at "Fenglong" (ST 40), "Zhongwan" (CV 12), "Guanyuan" (CV 4) and "Zusanli" (ST 36), with continuous wave, in frequency of 2 Hz, and current intensity of 1 mA, for 10 min each time. The rats in the sham EA group were intervened with EA at the points 5 mm next to the acupoints used in the EA group and no electricity was given; the sham EA was given for 10 min each time. Both the treatments were given once every other day for 8 weeks. The Lee's index and food intake were observed before the intervention as well as 2 weeks, 4 weeks, 6 weeks and 8 weeks into intervention; after the intervention, serum insulin (INS) and glucose infusion rate (GIR) were detected; serum cholecystokinin (CCK) level was detected by ELISA; c-fos expression in the area postrema (AP) and nucleus tractus solitarius (NTS) of medulla oblongata was detected by immunohistochemistry.@*RESULTS@#Before the intervention as well as 2 weeks, 4 weeks, 6 weeks and 8 weeks into intervention, the Lee's index and food intake in the model group were higher than those in normal group (<0.01). The Lee's index (6 weeks and 8 weeks into intervention) and food intake (4 weeks, 6 weeks and 8 weeks into intervention) in the EA group were lower than those in the model group and the sham EA group (<0.05, <0.01). After intervention, compared with the normal group, serum level of INS was increased (<0.01), while GIR, serum CCK level, c-fos expression in AP and NTS in the model group were decreased (<0.01, <0.05). Compared with the model group and the sham EA group, serum level of INS in the EA group was decreased (<0.01), and GIR, serum CCK level, c-fos expression in AP and NTS were increased (<0.01, <0.05).@*CONCLUSION@#EA of " acupoint combination" could effectively reduce appetite, body fat and enhance insulin sensitivity in obese rats with IR. The mechanism may be related to the regulation of central sensitivity of CCK.

10.
Article in Chinese | WPRIM | ID: wpr-847776

ABSTRACT

BACKGROUND: There is no uniform standard for acromioclavicular dislocation. Clavicular hook plate fixation is a commonly used fixation method clinically, but minimally invasive ligament reconstruction technology and arthroscopic surgery have become a research hotspot. OBJECTIVE: To observe the application value of suture bridge with thread anchor under arthroscopy in the treatment of acromioclavicular dislocation. METHODS: Patients with Tossy type III acromioclavicular dislocation who were admitted to Department of Orthopedic Surgery of General Hospital of Jizhong Energy Xingtai Mining Group between January 2016 and April 2018 were selected as the subjects. The patients were randomly divided into two groups. The patients in the arthroscopy group were treated with arthroscopic suture bridge technique with suture anchor for reduction and fixation, while those in the clavicle hook plate group were treated with clavicular hook plate for internal fixation. The length of surgical incision, duration of surgery, hospitalization time and time to return to work of both groups were recorded. The shoulder function was evaluated according to the Lazzcano criterion of curative effect at 3, 6 and 12 months after surgery. The visual analogue scale score of shoulder pain, abduction and raising, forward bend and raising were compared between the two groups. All patients were followed up for 1 year to record the occurrence of complications. This study was approved by the Medical Ethics Committee of General Hospital of Jizhong Energy Xingtai Mining Group. All subjects signed the informed consent. RESULTS AND CONCLUSION: (1) There was no significant difference in the duration of surgery between the two groups (P > 0.05). The surgical incision, hospitalization time and time to return to work of the arthroscopy group were significantly better than those of the clavicle hook plate group (P 0.05). (3) Visual analogue scale scores of the two groups showed a decreasing trend at 3, 6 and 12 months after surgery (P < 0.01). The visual analogue scale score of arthroscopy group was significantly lower than that of the clavicle hook plate group at the same time point (P < 0.01). (4) Angles of abduction and raising, forward bend and raising of both groups showed an increasing trend at 3, 6 and 12 months after surgery (P < 0.01). The angles of abduction and raising, forward bend and raising of the arthroscopy group were larger than those of the clavicle hook plate group at the same time point (P < 0.01). (5) The 1-year follow-up results showed that the incidence of complications in the arthroscopy group (6%) was significantly lower than that in the clavicle hook plate group (63%) (P < 0.01). (6) Compared with clavicular hook plate fixation, the technique of suture anchors under arthroscopy has the advantages of small trauma, quick recovery, fewer complications and no need to remove endografts again. Meanwhile, the surgery can also clearly diagnose the concomitant lesions in the joints and treat them when necessary, so as to improve the treatment effect.

11.
Article in Chinese | WPRIM | ID: wpr-774152

ABSTRACT

Microphone array based methods are gradually applied in the front-end speech enhancement and speech recognition improvement for cochlear implant in recent years. By placing several microphones in different locations in space, this method can collect multi-channel signals containing a lot of spatial position and orientation information. Microphone array can also yield specific beamforming mode to enhance desired signal and suppress ambient noise, which is particularly suitable to be applied in face-to-face conversation for cochlear implant users. And its application value has attracted more and more attention from researchers. In this paper, we describe the principle of microphone array method, analyze the microphone array based speech enhancement technologies in present literature, and further present the technical difficulties and development trend.


Subject(s)
Cochlear Implantation , Cochlear Implants , Humans , Speech , Speech Perception
12.
Article in Chinese | WPRIM | ID: wpr-745443

ABSTRACT

Objective To assess the imaging characteristics of 18F-Alfalide II in different tumorbearing mice and pharmacokinetics in Beagle dogs.Methods BALB/c nude mice(n-24)were used for subcutaneous tumor models(A549 and U87MG),orthotopic lung cancer models(A549)and orthotopic breast cancer models(MDA-MB-231)(n=6 in each group).18F-Alfatide II and 18F-fluorodeoxyglucose(FDG)microPET/CT images were compared in the 4 types of tumor-bearing nude mice models.18F-Alfatide II blocking experiment,biodistribution experiment and imaging studies in tumors of different growth cycles were performed in A549 subcutaneous tumor-bearing nude mice models.Pharmacokinetic experiments were carried out in Beagle dogs(n = 6)and CD-1 mice(n = 9).Two-sample t test was used to analyze the data.Results Compared with 18F-FDG,18F-Alfatide II microPET/CT images showed better imaging quality and contrast in subcutaneous A549,U87MG tumors and orthotopic A549(tumor/heart:4.50±1.17 vs 0.95±0.31;t = 4.125,P<0.01),orthotopic MDA-MB-231(tumor/muscle:6.60±1.53 vs 0.92±0.43;t = 3.984,P<0.01)transplantation nude mice models.18F-Alfatide II could specifically target A549 tumors,and the tumor uptake of 18F-Alfatide II was reduced by about 75% after pre-injection with cyclo(Arg-Gly-Asp-D-Tyr-Lys)(c(RGDyk)).18F-Alfatide II was rapidly cleared from the blood of Beagle dogs(T1/2 was(57.34±11.69)min).It was cleared in the form of prototype drug and(69.24±6.82)% of cumulative dose was excreted through the urine within 4 h after administration.Conclusions 18F-Alfatide II shows a higher target/non-target ratio than,18F-FDG in the imaging of A549,MDA-MB-231 and U87MG tumor-bearing nude mice models,which is more conducive to the diagnosis of tumor.18F-Alfatide II has excellent pharmacokinetic properties.

13.
Article in Chinese | WPRIM | ID: wpr-733709

ABSTRACT

Objective To investigate the efficacy of domestic porcine antihuman lymphocyte immunoglobulin (p-ALG) in the treatment of severe aplastic anemia (SAA) with allogeneic hemopoietic stem cell transplantation (HSCT). Methods The clinical data of 5 SAA patients who received allogeneic HSCT from January 2015 to May 2018 were retrospectively analyzed. The conditioning regimen included p-ALG + cyclophosphamide + fludarabine. The method of peripheral stem cell and bone marrow blood was used in allogeneic HSCT (the total amount of bone marrow blood was less than 400 ml and the puncture point was not replaced). The p-ALG related complications, post-transplantation hemopoietic stem cell reconstitution time and efficacy were recorded. Results Allergic reaction occurred in 1 patient when using p-ALG, and there was no serum reaction. Hemopoietic reconstitution was achieved in all the 5 patients. The time for neutrophilic granulocyte > 0.5 × 109/L was 11 to 17 d, and the time for platelet count > 20 × 109/L was 11 to 15 d after transplantation. The results of short-strand repeat polymerase chain reaction assays showed all complete donor chimera. Graft versus host disease occurred in 3 cases, and was successfully controlled by methylprednisolone and tacrolimus. The time for stopping red blood cell transfusion was 9 to 87 d. The patients were followed up for 1 to 37 months, and the patients all survived well. Conclusions The efficacy of p-ALG in SAA patients of allogeneic HSCT is affirmative, and the cost is obviously reduced. It is worthy of clinical use.

14.
International Journal of Surgery ; (12): 107-112,封3, 2019.
Article in Chinese | WPRIM | ID: wpr-732796

ABSTRACT

Objective To observe the role of lycopene played in the liver ischemia reperfusion injury,and its effort on autophagy.Methods Eighty SPF male SD rats(Animal Laboratory of the Yang Zhou University) were divided into 4 groups by random number table.Sham-operated group:after anesthesia,just opened the abdominal cavity.Treatment group:after molding,lycopene (10 mg/kg) was dissolved in medicinal corn oil (2 ml/kg) and intraperitoneal injection was performed.The lycopene was injected 60 minutes before surgery.Control group:after molding,intraperitoneal injection of equal volume of normal saline was given.Inhibiting group:lycopene (10 mg/kg) + 3-ma (15 mg/kg) were injected into the abdominal cavity after injection.20 rats in each group.The determination of sample collection and observation of indicators at 1 h,6 h,12 h,24 h after ischemia reperfusion.The serum ALT and AST levels were checked.The mRNA expression of TNF-α and IL-6 was measured by real-time PCR and the expression of Beclin-1,was detected by western blotting.Hematoxylin and Eeosin staining was used to evaluate the pathological change of liver tissues.The measurement data were expressed as (Mean ± SD),comparisons between the two groups were analyzed by independent sample t test,and comparisons among groups were analyzed by One-way anova and Newman-keuls method.Results The serum ALT levels of Sham-operated group in 1 h,6 h,12 h,24 h were (39.81 ±13.54),(39.65 ±14.24),(39.61 ±13.68),(38.72± 12.85) U/L,the serum ALT levels of liver ischemia-reperfusion control group in 1 h,6 h,12 h,24 h were (128.43 ± 33.49),(637.26 ± 89.53),(289.76 ± 43.59),(124.26 ± 35.32) U/L.Compared with Shamoperated group,the serum ALT levels of liver ischemia-reperfusion control group were significantly increased (P < 0.05).The serum ALT levels of treatment group in 1 h,6 h,12 h,24 h were (73.26 ± 23.24),(419.27 ± 56.49),(196.23 ± 26.23),79.76 ± 20.43) U/L.Compared with liver ischemia-reperfusion control group,the serum ALT levels of treatment group were significantly declined (P < 0.05).The serum ALT levels of inhibiting group in 1 h,6 h,12 h,24 h were (142.59 ±42.86),(592.45 ±48.36),(268.23 ±42.36),(131.23 ± 24.36) U/L.Compared with treatment group,the serum ALT levels of treatment group were significantly increased (P < 0.05).Compared with liver ischemia-reperfusion control group,the serum AST levels and the expression of inflammatory factor TNF alpha and IL-6 of treatment group were significantly declined(P < 0.05).The results of light microscope indicated that treatment groups significantly reduce the liver cell damage compared with liver ischemia-reperfusion control group.The lycopene could promote the autophagy of mice which related to protein of Beclin-1.Drugs of 3-ma which inhibit autophagy,reduces the expression of Beclin-1.Conclusion Lycopene preconditioning can protect liver from ischemia reperfusion injury by promoting autophagy in liver cells.

15.
Article in Chinese | WPRIM | ID: wpr-905716

ABSTRACT

This article reviewed the changes of ankle muscle strength after lower limb joint injuries and the isokinetic test mode which could reflect this characteristic better. The varus plantarflexion strength decreased significantly after plantarflexion injury of ankle joint. The dorsiflexor strength of ankle joint could not reach the level of the healthy side for a long time after anterior cruciate ligament rupture and Achilles tendon injury. Ankle isokinetic muscle test included testing direction, muscle contraction form and testing speed. It is meaningful to test varus/valgus and plantar/dorsal flexion in ankle joint sprain and functional ankle instability; however, the muscle contraction form and testing speed have little effect on the result.

16.
Article in Chinese | WPRIM | ID: wpr-837676

ABSTRACT

Objective To investigate the malaria vectors in Nanyang City. Methods Two townships were selected as malaria vector surveillance sites in Nanyang City from 2005 to 2017, including Mazhenfu Township in Tanghe County and Huaiyuan Township in Tongbai County, and the malaria vectors were investigated using the semi-overnight human baiting trapping method and mosquito trapping in nets. Results The mean density of Anopheles sinensis was 0.65 to 22.30 mosquitos/(person · hour) in the two surveillance sites in Nanyang City using the human baiting trapping method from 2005 to 2017, and the peak density was detected in June and July. An. anthropophagus was captured only in Tongbai County during the period between 2008 and 2011, with a mean density of 0.03 to 1.10 mosquitos/(person · hour). The mean density of An. sinensis was 0.08 to 0.24 mosquitos/(net · night) using the mosquito trapping in nets from 2005 to 2011, and the peak density was detected in July and August, while few An. anthropophagus captured only in Tongbai County, with a mean density of 0.01 to 0.02 mosquitos/(net · night). Conclusions There are malaria vectors in Nanyang City, including An. sinensis and An. anthropophagus, and An. sinensis is the dominant mosquito species.

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Article in Chinese | WPRIM | ID: wpr-702249

ABSTRACT

Objective To investigate the surgical treatment of lung cancer invading the heart and large blood vessels.Methods The clinical data of 96 cases of lung cancer invading the heart and large blood vessel admitted into our hospital from March 2009 to March 2012 were retrospectively analyzed,especially the surgical methods,postoperative complications and 5-year survival rate.Results The 5-year survival rate of lung cancer patients who infringed the atrium and superior vena cava had no significant difference (P > 0.05).However,the 5-year survival rate and median survival time of lung cancer patients who infringed the atrium and superior vena cava were significantly lower than those of lung cancer patients who infiltrated the pulmonary artery,and the difference was statistically significant(P < 0.05).The median survival time of patients with lung cancer invading the atrium was lower than that of patients with lung cancer invading the superior vena cava,and the difference was statistically significant(P < 0.05).The range of tumor involvement after operation was significantly lower than that before operation,and the difference was statistically significant(P < 0.05).Three types of surgical methods were used for patients according to different invading sites,and the complication incidence of 3 surgical methods were similar with no statistically significant difference(P > 0.05).Conclusion Surgical treatment for lung cancer invading the heart and large blood vessels has a good efficacy,which can improve the involved tumor range of patients without serious postoperative complications and provide opportunity for patients with comprehensive postoperative treatment.In addition,it can significantly improve the 5-year survival rate of patients.

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Article in Chinese | WPRIM | ID: wpr-701873

ABSTRACT

Objective To investigate the changes of fibrinogen and D -dimer levels in patients with severe burn and their relationship with prognosis .Methods 90 patients with severe burn were selected as the subjects .The patients were divided into the dead group (30 cases) and the survival group (60 cases) according to the prognosis of the patients.The differences and changes of fibrinogen and D -dimer at the same time after admission were compared between the two groups .Results At each time point after admission , the fibrinogen levels of the death group were significantly higher than those of the survival group at the same time [(5.9 ±1.5)g/L vs.(5.1 ±1.3)g/L,(8.8 ± 2.2)g/L vs.(7.4 ±2.5)g/L,(7.6 ±2.8)g/L vs.(5.7 ±1.2)g/L,(7.1 ±2.5)g/L vs.(4.3 ±1.7)g/L],the differences were statistically significant(t =3.892,4.762,5.436,6.987,all P <0.05).At each time point after admission,the D-dimer levels of the death group were significantly higher than those of the survival group at the same time[(743.4 ±226.2)ng/mL vs.(704.1 ±214.6)ng/mL,(935.4 ±256.9)ng/mL vs.(834.6 ±256.2)ng/mL, (846.3 ±291.5)ng/mL vs.(712.1 ±251.3) ng/mL,(756.7 ±232.8) ng/mL vs.(601.5 ±168.7) ng/mL],the differences were statistically significant (t=3.895,5.477,4.743,5.746,all P<0.05).The level of D-dimer in the survival group showed a rise firstly ,then showed a decrease trend after admission ,while the levels of fibrinogen and D-dimer of the death group were altered in double -peak change .Conclusion There is an obvious impairment of coagulation function in severe burn patients , and the level of D -dimer and fibrinogen is higher , the prognosis of patients is worse .

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Article in Chinese | WPRIM | ID: wpr-692685

ABSTRACT

Objective To explore the diagnostic value of serum lipoprotein(a)[Lp(a)]in cerebral infarction by a model of Logistic regression and receiver operating characteristic(ROC)curve.Methods A total of 316 patients with cerebral thrombosis from Foshan Hospital Affiliated to Southern Medical University were col-lected.According to the diagnostic criteria,the patients were divided into cerebral thrombosis group(196 ca-ses)and non-cerebral thrombosis group(120 cases).All the subjects were tested for Lp(a)by immune turbi-dimetry.To evaluate the diagnostic value of Lp(a)by applying logistic regression model,drawing ROC curves and calculating the area under the curve(AUC).Results The P25,P50,P75of Lp(a)in cerebral thrombosis group and non-cerebral thrombosis group were 97.23,238.22,430.01 and 29.80,92.27,233.86,the average rank were 185.42 and 114.52,the differences in the two groups were significant(P<0.05).Logistic regres-sion showed that the correlation between Lp(a)level and cerebral thrombosis was positive,the partial regres-sion coefficient(B)was 0.005,Wald value was 31.295.It suggested that when the levels of Lp(a)was higher the risk of cerebral thrombosis increased.The most valuable diagnosis level was 305.80 mg/L.And the area under the ROC curve(AUC)was 0.724,which has moderate diagnostic efficacy.Diagnostic specificity was 91.7%,misdiagnosis rate was 8.3%,negative predictive value was 48.7%,sensitivity was 40.8%,omission rate was 59.2%,positive predictive value was 88.9%.Conclusion The level of serum Lp(a)has high diag-nostic specificity for the diagnosis of cerebral thrombosis.

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Article in Chinese | WPRIM | ID: wpr-692346

ABSTRACT

The chemical vapor deposition grown graphene was transferred onto flexible polyethylene terephthalate surface to fabricate a graphene platform electrode ( GPE) , and gold nanoparticles ( AuNPs) were electrodeposited on GPE to form an AuNPs modified GPE ( AuNPs/GPE ) . The formation of AuNPs was confirmed by scanning electron microscopy ( SEM) , energy dispersive spectrometer ( EDS) , high-resolution transmission electron microscope ( HR-TEM) , X-ray diffraction ( XRD) and Raman spectra. On AuNPs/GPE, dopamine ( DA ) displayed a pair of well-defined redox peaks with highly enhanced peak currents compared with those on GPE. At detection potential of 0. 20 V, AuNPs/GPE sensor presented a wide linear range of 0. 1 μmol/L to 400. 0 μmol/L of DA with a detection limit of 3. 9 nmol/L (S/N=3). In addition, the proposed sensor allowed highly selective and sensitive, stable and fast amperometric sensing of DA.

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