Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 59
Filter
1.
Article in Chinese | WPRIM | ID: wpr-879158

ABSTRACT

This research was used with high performance liquid chromatography(HPLC), combined with information entropy-response surface method(RSM) to investigate the ethanol concentration, extraction time, liquid-to-material ratio. Taking the content of four chromogens as evaluation indexes, the weight coefficients of each index were given, and the comprehensive score was calculated to optimize the extraction process. Then, prim-O-glucosylcimifugin was used as the reference, the relative calibration factors(RCFs) of cimifugin, 4'-O-β-D-glucosyl-5-O-methylvisamminol and sec-O-glucosylhamaudo to prim-O-glucosylcimifugin were calculated respectively. The contents of four components in Saposhnikoviae Radix were determined by both external standard method(ESM)and quantitative analysis of multi-components by single marker(QAMS) method, and the results were compared. At last, combined with principal component analysis(PCA) and orthogonal partial least squares discriminant analysis(OPLS-DA) to evaluate the quality of the Saposhnikoviae Radix in different production areas. The optimal extraction process parameter of the Saposhnikoviae Radix was as follows: liquid-to-material ratio is 60∶1(mL·g~(-1)), extraction time is 35 min, and ethanol concentration is 70%. The repeatability of the RCFs was perfect, and the results calculated by the QAMS were consistent with the results from the ESM. The stoichiometric results indicate that there are obvious differences in the distribution of Saposhnikoviae Radix in different production areas, and cimifugin and prim-O-glucosylcimifugin are the characteristic compounds that cause this difference. In this study, the optimal extraction process is stable and feasible, and the method of QAMS is accurate and reliable. From the perspective of four chromogens, there are differences in the quality of the Saposhnikoviae Radix in different production areas. Therefore, the established extraction process combined with the method of QAMS can be used to evaluate the quality of Saposhnikoviae Radix and provide a scientific basis for the quality control of Saposhnikoviae Radix.


Subject(s)
Apiaceae , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Entropy , Plant Roots
2.
Article in Chinese | WPRIM | ID: wpr-837686

ABSTRACT

@#Congenital coronary artery anomalies include various congenital anatomic anomalies of coronary artery. Severe congenital coronary artery anomalies usually result in ventricular dysfunction, myocardium ischemia, and even sudden death. Hence, understanding the coronary artery anomalies is critically important for the related surgeons to make reasonable strategies. This consensus is based on current literature and opinions of Chinese experts. And we mainly discuss the anatomy, clinical manifestation, diagnosis and treatment of important congenital coronary artery anomalies in clinics including anomalous aortic origin of a coronary artery, anomalous origin of the coronary artery from the pulmonary artery and coronary fistula.

3.
Journal of Medical Postgraduates ; (12): 609-612, 2020.
Article in Chinese | WPRIM | ID: wpr-821837

ABSTRACT

ObjectiveAt present, there are few reports on the therapeutic effect of probiotic supplements in patients with dietary-controlled gestational diabetes mellitus (GDM). This study aims to evaluate the effect of probiotic supplements on insulin resistance in patients with dietary-controlled GDM.Methods122 pregnant women with dietary-controlled GDM who could control blood glucose less than 92 mg/dL through diet and exercise were selected from the Obstetrics Department in Bayannur Hospital from February to December 2018. The patients were randomly divided into two groups: Probiotics Group (probiotic supplements containing bifidobacterium and lactobacillus) and Placebo Group (placebo capsules). 61 patients in each group were treated continuously for 4 weeks. The main evaluation index was the mean difference of fasting blood glucose, fasting plasma insulin and insulin resistance index (HOMA-IR) between the two groups, and the secondary evaluation index was the change of maternal weight after intervention.ResultsThe increase of fasting blood glucose [(0.59±6.42)mg/dL], fasting plasma insulin [(1.14±1.95)mIU/mL] and HOMA-IR (0.27±0.45) in the Probiotics Group after intervention were significantly lower than those in the Placebo Group [(4.78±7.47 mg/dL), (3.86±1.82) mIU/mL, (0.86±0.59)], and the difference was statistically significant (P 0.05).ConclusionDuring pregnancy, probiotic supplements for four weeks in patients with dietary-controlled GDM can reduce fasting blood glucose and increase insulin sensitivity. Therefore, probiotic supplements can be used as adjunctive therapy for blood glucose control in patients with dietary-controlled GDM.

4.
Journal of Medical Postgraduates ; (12): 609-612, 2020.
Article in Chinese | WPRIM | ID: wpr-821817

ABSTRACT

ObjectiveAt present, there are few reports on the therapeutic effect of probiotic supplements in patients with dietary-controlled gestational diabetes mellitus (GDM). This study aims to evaluate the effect of probiotic supplements on insulin resistance in patients with dietary-controlled GDM.Methods122 pregnant women with dietary-controlled GDM who could control blood glucose less than 92 mg/dL through diet and exercise were selected from the Obstetrics Department in Bayannur Hospital from February to December 2018. The patients were randomly divided into two groups: Probiotics Group (probiotic supplements containing bifidobacterium and lactobacillus) and Placebo Group (placebo capsules). 61 patients in each group were treated continuously for 4 weeks. The main evaluation index was the mean difference of fasting blood glucose, fasting plasma insulin and insulin resistance index (HOMA-IR) between the two groups, and the secondary evaluation index was the change of maternal weight after intervention.ResultsThe increase of fasting blood glucose [(0.59±6.42)mg/dL], fasting plasma insulin [(1.14±1.95)mIU/mL] and HOMA-IR (0.27±0.45) in the Probiotics Group after intervention were significantly lower than those in the Placebo Group [(4.78±7.47 mg/dL), (3.86±1.82) mIU/mL, (0.86±0.59)], and the difference was statistically significant (P 0.05).ConclusionDuring pregnancy, probiotic supplements for four weeks in patients with dietary-controlled GDM can reduce fasting blood glucose and increase insulin sensitivity. Therefore, probiotic supplements can be used as adjunctive therapy for blood glucose control in patients with dietary-controlled GDM.

5.
Acta Pharmaceutica Sinica ; (12): 2277-2281, 2019.
Article in Chinese | WPRIM | ID: wpr-780342

ABSTRACT

The chemical constituents were isolated and purified by column chromatography and semi-preparative reversed-phase high performance liquid chromatography with silica gel, MCI and polyamide in order to study the chemical constituents of dried flowers of Osmanthus fragrans var. aurantiacus. Their structures were identified by the physical and chemical properties and one-dimensional nuclear magnetic resonance (1H-, 13C-NMR, DEPT), two-dimensional nuclear magnetic resonance (1H-1H COSY, non-decoupled HSQC, HSQC, HMBC), UV, IR and high resolution mass spectrometry data. One new compound (1) and five known compounds (2-6) were isolated from 95% ethanol extract of dried broccoli. They were identified as (9S)-9-hydroxymengastigm-5-en-4-one-9-O-primeveroside (1), oleanolic acid (2), forsythiaside (3), 2-(4-hydroxyphenethyl)-ethanol-(6-acetyl)-β-D-glucopyranoside (4), salidroside (5), and acteoside (6). Compounds (2-6) were isolated from this plant for the first time.

6.
Article in Chinese | WPRIM | ID: wpr-861399

ABSTRACT

Objective To compare the diagnostic performances of ultrasonography and digital breast tomosynthesis (DBT) in women with dense breasts. Methods A total of 148 women (148 breast lesions) with dense breasts underwent DBT and ultrasonography. Then the patients were divided into groups according to the composition of the breast, patients' age, lesion size and with or without calcification. Taking pathological results as gold standards, ROC curve was used to evaluate the diagnostic performances of ultrasonography DBT, and the AUC, sensitivity and specificity of them were compared. Results AUC of ultrasonography and DBT in diagnosis of dense breast lesions was 0.956 and 0.815 (Z=3.866, P40-55 years (0.946 vs 0.801, P=0.013) groups. According to clinical diagnostic criteria, the sensitivity of ultrasonography and DBT in diagnosis of benign and malignant lesions in dense breast was 94.32% (83/88) and 93.18% (82/88, χ2=0.096, P=1.000), and the specificity was 83.33% (50/60) and 61.67% (37/60, χ2=7.060, P=0.007). Conclusion Ultrasonography shows better diagnostic performance than DBT in women with dense breast. Ultrasonography is suitable to be chosen as the preferred examination in women with small lesions in heterogeneously dense breasts and aged ≤55 years than DBT.

7.
Article in Chinese | WPRIM | ID: wpr-699391

ABSTRACT

Recently,many studies have suggested that bone sclerostin exists in atherosclerotic plaques,calcified ves-sels and valve tissue.The level of circulating bone sclerostin is closely associated with diabetes mellitus,coronary heart disease and their risk factors.The present article made a review on the relationship among atherosclerosis, coronary heart disease,their risk factors and bone sclerostin on the basis of the characteristics and influencing fac-tors of bone sclerostin.

8.
Article in Chinese | WPRIM | ID: wpr-692991

ABSTRACT

Objective To investigate the correlation between serum interleukin-6 (IL-6) to albumin ratio (IAR) at admission and early outcome of patients with acute noncardioembolic ischemic stroke.Methods From January 2013 to May 2015,patients with acute noncardioembolic ischemic stroke admitted to the Department of Neurology,the First Affiliated Hospital of Xi'an Jiaotong University were enrolled retrospectively.Three months after onset,the clinical outcome were assessed by the modified Rankin scale (mRS),0-2 was good outcome,and > 2 was poor outcome.The baseline clinical data,laboratory tests,and IAR in both groups were compared.Multivariate logistic regression analysis was used to determine the independent risk factors affecting early outcomes.Receiver operating characteristic (ROC) curve was used to evaluate the predictive value of IAR for early outcome.Results A total of 236 patients were enrolled in the study,including good outcome in 143 (60.6%) and poor outcome in 93 (39.4%).Univariate analysis showed that age (62.99 ± 11.34 years vs.59.62 ± 11.83 years;t =-2.176,P=0.031),serum IL-6 (37.56 ± 3.82 ng/L vs.34.82 ± 3.13 ng/L;t =-6.016,P < 0.001),IAR (1.02 ± 0.08 vs.0.93 ± 0.07;t =-9.474,P<0.001),and the proportions of patients with severe stroke (6.5% vs.0%;x2 =8.142,P =0.012) and combined CHD (30.1% vs.18.2%;x2 =4.542,P =0.033) of the poor outcome group were significantly higher than those of the good outcome group.Multivariate logistic regression analysis showed that high IAR at admission was an independent risk factor for early poor outcome (odds ratio,5.192,95% confidence interval 3.283-8.213;P <0.001).ROC curve analysis showed the optimal cut-off value of IAR predicting for early outcome was 1.00,and the area under the ROC curve was 0.807 (95% confidence interval 0.748 to 0.860).The sensitivity was 62.4%,the specificity was 88.1%,the positive predictive value was 69.2%,the negative predictive value was 79.3%,and the accuracy was 75.4%.Conclusions High serum IAR at admission was an independent risk factor for early poor outcome in patients with acute noncardioembolic ischemic stroke.It had a certain predictive value for the outcomes.

9.
Article in Chinese | WPRIM | ID: wpr-710847

ABSTRACT

Objective To assess the application of preoperative prognostic nutritional index (PNI) in evaluation the surgical risk and long-term prognosis for elderly patients with gastric cancer.Methods The clinical data of 205 patients aged ≥ 75 years with gastric cancer undergoing radical resection from January 2004 to December 2016 were analyzed retrospectively.The PNI value was calculated by serum albumin (g/L) +5 x lymphocyte count (x 109/L).The receiver operating characteristic (ROC) curve and Youden's index was used to determine the value of PNI in surgical risk and prognosis of patients.Patients were divided into low PNI group and high PNI group based on the cut-off value,the clinicopathological characteristics,postoperative complications and long-term survival were compared between two groups.Results The average PNI value of 205 patients was 47.3 ± 5.5.When 44.9 was set as the cut-off value with the maximal Youden's index,the sensitivity and specificity of PNI were 0.86 and 0.47,respectively.There were statistically significant differences between the two groups of patients in age(t =-2.16,P =0.032),BMI (t =4.88,P =0.000),Charlson comorbidity score (x2 =7.77,P =0.005),gastric resection range (x2 =8.63,P =0.003),postoperative complications (x2 =9.46,P =0.002).The incidence of complications in the high PNI group was 24.8% (33/133),which was lower than that in the low PNI group (45.8%,33/72;x2 =9.46,P =0.002).Multivariate logistic analysis showed that age (P =0.032),Charlson coplications scal (CCS) (P =0.042) and PNI < 44.9 (P =0.027) were independent risk factors for postoperative complications;PNI < 44.9 (P =0.001),gastrectomy (P =0.011),lymph node dissection (P =0.000),tumor differentiation (P =0.001) and TNM stage (P =0.000) were independent prognostic factors for elderly patients with gastric cancer.Conclusions Prognostic nutritional index is a valuable clinical marker in evaluation of surgical risk and prognosis for elderly patients with gastric cancer.

10.
Journal of Experimental Hematology ; (6): 1033-1037, 2018.
Article in Chinese | WPRIM | ID: wpr-689533

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the therapeutic safety and efficacy of VDMP re-induction regimen in Chinese children with relapsed acute lymphoblastic leukemia (ALL).</p><p><b>METHODS</b>Forty-one patients with relapsed ALL were prospectively enrolled in this study. All the patients were distributed in 3 children's hospitals and treated with VDMP regimen as the first re-induction chemotherapy. Therapeutic efficacy and side-effects were analyzed.</p><p><b>RESULTS</b>The ratio of male to female was 27:14. The median age was 7.9 (2.2-15.4) years old. Patients relapsed at very early, early, and late stage were 7 cases, 11 cases, and 23 cases, respectively.The immunophenotype analysis showed that 38 cases were B-ALL, and 3 cases were T-ALL. All patients suffered from grade 4 of neutropenia and forty(97.6%) cases got infection, of them one case died. Thirty-nine(95.1%) cases had nonhematologic adverse event at least one organ involved grade 3 in 38 out of 41 cases, the VDMP therapy was completed, 34(89.5%) cases achieved a complete remission (CR), 1 case achieved partial remission(PR), and 3 cases didn't get remission. Follow-up data of 38 cases with completing VDMP chemotherapy were obtained, only one case was lost. Among 37 cases available for evaluation, 16 cases received allo-hematopoietic stem cell transplantation(allo-HSCT) after chemotherapy, and 13 patients survived, while 21 cases did not receive allo-HSCT(treated with chemotherapy only), and 8 patients survived.The overall survival rate of allo-HSCT group was significantly higher than that of those treated with chemotherapy only(P<0.05).</p><p><b>CONCLUSION</b>VDMP re-induction regimen is effective and well tolerable for pafients in the treated children with relapsed ALL. After remission, allo-HSCT is recommended with the aim of long survival.</p>


Subject(s)
Adolescent , Antineoplastic Combined Chemotherapy Protocols , Child , Child, Preschool , Female , Hematopoietic Stem Cell Transplantation , Humans , Induction Chemotherapy , Male , Precursor Cell Lymphoblastic Leukemia-Lymphoma , Remission Induction , Treatment Outcome
11.
Article in Chinese | WPRIM | ID: wpr-702403

ABSTRACT

The dense breast notification legislation,which was passed by Connecticut in 2009,has attracted more attention to the dense breast.Now there is still no unified criterion nor global guide for reference to indicate additional supplemental screening tool suitable for women with dense breast.Ultrasonography and digital breast tomosynthesis became research focus.The progresses of these two technologies in dense breast were reviewed in this article.

12.
National Journal of Andrology ; (12): 821-827, 2017.
Article in Chinese | WPRIM | ID: wpr-812871

ABSTRACT

Objective@#To study the protective effect of Qilin Pills (QLP) on the reproductive function of rats with oligoasthenospermia (OAS) induced by tripterygium glycosides.@*METHODS@#Twenty-eight male SD rats were randomly divided into a normal control, an OAS model control, a low-dose QLP, and a high-dose QLP group of equal number. OAS models were made in the latter three groups by intragastrical administration of tripterygium glycosides at 40 mg per kg of the body weight per day, and meanwhile the animals in the low- and high-dose QLP groups were treated with QLP at 1.62 and 3.24 g per kg of the body weight per day, respectively, while those in the OAS model group with normal saline, all for 30 consecutive days. Then all the rats were executed for obtaining the testis weight, testis viscera index, epididymal sperm concentration and motility, reproductive hormone levels, and antioxidation indexes and observation of the histomorphological changes of the testis tissue by HE staining.@*RESULTS@#After 30 days of intervention, the low- and high-dose QLP groups, as compared with the OAS model controls, showed significantly improved epididymal sperm concentration ([14.57 ± 3.95] and [39.71 ± 11.31] vs [4.71 ± 1.25] ×10⁶/ml, P <0.05) and motility ([3.71 ± 1.11] and [4.29 ± 1.80] vs [0.57 ± 0.53]%, P <0.05), increased levels of sex hormone binding globulin (SHBG) ([94.83 ± 11.17] and [88.05 ± 9.21] vs [56.74 ± 8.29] nmol/L, P <0.05) and free testosterone (FT) ([27.27 ± 3.63] and [32.80 ± 2.51] vs [22.81 ± 2.75] nmol/L, P <0.05), decreased level of follicle-stimulating hormone (FSH) ([1.49 ± 0.62] and [1.12 ± 0.83] vs [1.71 ± 0.52] mIU/ml, P <0.05), but no significant change in the total testosterone (TT) level. Meanwhile, the level of superoxide dismutase (SOD) was markedly elevated in the low- and high-dose QLP groups in comparison with the OAS model control group ([277.14 ± 15.84] and [299.60 ± 20.83] vs [250.04 ± 31.06] U/ml, P <0.05) while that of reactive oxygen species (ROS) remarkably reduced ([397.61 ± 62.71] and [376.84 ± 67.14] vs [552.20 ± 58.07] IU/ml, P <0.05). HE staining showed that QLP intervention significantly increased the layers and quantity of spermatogenic cells in the testicular seminiferous tubules of the OAS rats.@*CONCLUSIONS@#QLP can effectively protect the reproductive system of oligoasthenospermia rats by raising sperm quality, elevating reproductive hormone levels, reducing oxidative stress injury, and improving histomorphology of the testis.


Subject(s)
Animals , Asthenozoospermia , Drug Therapy , Drugs, Chinese Herbal , Pharmacology , Epididymis , Follicle Stimulating Hormone , Male , Oligospermia , Drug Therapy , Protective Agents , Pharmacology , Random Allocation , Rats , Rats, Sprague-Dawley , Reproduction , Seminiferous Tubules , Sperm Count , Spermatozoa , Superoxide Dismutase , Testis , Testosterone , Blood , Tripterygium
13.
Chinese Journal of Immunology ; (12): 1636-1641, 2017.
Article in Chinese | WPRIM | ID: wpr-669091

ABSTRACT

Objective:To investigate the effect of regulating platelet function on systemic inflammatory response and pulmonary oxygenation function in rats during extracorporeal circulation(ECC).Methods:The rats participating in the ECC model were randomly divided into ECC supplemented platelet group (group MH),ECC supplemented plasma group (group MP),and continuous pump Tirofiban+ECC supplemented platelet group (group TMH),with 8 rats in each group.Rats of the three groups lasted two hours with ECC,observed for two hours after operation.Enzyme linked immunosorbent assay (ELISA) was used to detect inflammatory factors in the plasma and bronchoalveolar lavage fluid of rats in the three groups.The oxygenation index and lung tissue water content were meas-ured.The number of circulating endothelial quantity was measured by flow cytometry,and pathological changes of lung tissue in rats were observed by hematoxylin eosin staining.The effects of regulating platelet function on the levels of systemic inflammatory response, the degree of vascular endothelial damage,the edema degree of lung tissue and pulmonary oxygenation function of ECC rats were analyzed.Results:There was no significant difference in platelet count between group MH and group MP after two hours with ECC(P>0.05),the group TMH was significantly higher than that in group MH and group MP (P<0.05).After two hours with ECC,the oxygenation indexes of the three groups were lower than that at the beginning of ECC(P<0.05).There was no significant difference in lung tissue water content between the three groups(P>0.05);the level of neutrophil elastase(NE) in bronchoalveolar lavage fluid of rats in group MH was lower than that in group TMH(P<0.05),the level of NE in plasma of group TMH was lower than that in group MP (P<0.05).At the end of ECC,there were no statistically significant differences in circulating endothelial quantity of the three groups (P>0.05).Pathological examination of lung tissue showed that the lung interstitium of group MH was thinner than that of other groups,and inflammatory cell infiltration was less.Conclusion:At the early stage of ECC,supplement fresh platelets after platelet sharp decrease can significantly reduce the inflammatory response in the circulation.The protective effect may be related to platelets adhere to the endothelium associated with ECC related inflammatory reactions,and decrease the neutrophils and endothelial tissue adhesion.It is suggested that certain number of platelets with normal adhesion and aggregation function in ECC may reduce inflammatory response and lung injury after operative,and improve prognosis.

14.
Article in Chinese | WPRIM | ID: wpr-662911

ABSTRACT

BACKGROUND: iRegene collagen sponge exhibits stable physical and chemical properties, and has passed the test by the State Food and Drug Administration of China. OBJECTIVE: To study the hemostatic effect and the biocompatibility of the iRegene collagen sponge on a liver wound by means of rat models. METHODS: Liver trauma bleeding models were made in 21 Sprague-Dawley rats. These model rats were randomized into three groups (n=7 per group): experimental group with implantation and external application of iRegene collagen sponge; positive control group with implantation of medical collagen sponge and external application of iRegene collagen sponge; blank control group with external application of medical gauze. The bleeding time and amount on the liver wounds were observed. Histological observation on the liver wound was performed at 7, 14, 28 days after intervention. RESULTS AND CONCLUSION: The bleeding time was shorter in the experimental group than the positive control group (P ≤ 0.05). Beyond that, there was no difference in the bleeding amount and time among the three groups. Histological findings on the liver wound showed that the iRegene collagen sponge in the experimental group was completely wrapped with fibrous connective tissues and began to degrade at 7 days after intervention, the Inflammatory cell infiltration mainly occurred in neutrophils, and new capillaries were observed in peripheral connective tissues; at 14 days after intervention, the fibrous connective tissues became remarkably thickened, the number of neurophils was reduced, and the number of macrophages was increased; at 28 days after intervention, the iRegene collagen sponge degraded completely, most of the liver tissues recovered, and there were macrophages, monocytes, fibroblasts and capillaries in the inflammatory connective tissues adjacent to a part of liver tissues. Similar findings were observed in the positive control group. In the blank control group, there were obvious connective tissues on the wound and red blood cells in the liver sinus, and occasionally liver tissue bleeding and vacuolar degeneration were visible; at 28 days after intervention, there were thickened connective tissues on the wound, red blood cells in the liver sinus and reversed hepatic stellate cells. To conclude, the iRegene collagen sponge possesses effective hemostatic effects on liver wounds and shows good histocompatibility.

15.
Article in Chinese | WPRIM | ID: wpr-660998

ABSTRACT

BACKGROUND: iRegene collagen sponge exhibits stable physical and chemical properties, and has passed the test by the State Food and Drug Administration of China. OBJECTIVE: To study the hemostatic effect and the biocompatibility of the iRegene collagen sponge on a liver wound by means of rat models. METHODS: Liver trauma bleeding models were made in 21 Sprague-Dawley rats. These model rats were randomized into three groups (n=7 per group): experimental group with implantation and external application of iRegene collagen sponge; positive control group with implantation of medical collagen sponge and external application of iRegene collagen sponge; blank control group with external application of medical gauze. The bleeding time and amount on the liver wounds were observed. Histological observation on the liver wound was performed at 7, 14, 28 days after intervention. RESULTS AND CONCLUSION: The bleeding time was shorter in the experimental group than the positive control group (P ≤ 0.05). Beyond that, there was no difference in the bleeding amount and time among the three groups. Histological findings on the liver wound showed that the iRegene collagen sponge in the experimental group was completely wrapped with fibrous connective tissues and began to degrade at 7 days after intervention, the Inflammatory cell infiltration mainly occurred in neutrophils, and new capillaries were observed in peripheral connective tissues; at 14 days after intervention, the fibrous connective tissues became remarkably thickened, the number of neurophils was reduced, and the number of macrophages was increased; at 28 days after intervention, the iRegene collagen sponge degraded completely, most of the liver tissues recovered, and there were macrophages, monocytes, fibroblasts and capillaries in the inflammatory connective tissues adjacent to a part of liver tissues. Similar findings were observed in the positive control group. In the blank control group, there were obvious connective tissues on the wound and red blood cells in the liver sinus, and occasionally liver tissue bleeding and vacuolar degeneration were visible; at 28 days after intervention, there were thickened connective tissues on the wound, red blood cells in the liver sinus and reversed hepatic stellate cells. To conclude, the iRegene collagen sponge possesses effective hemostatic effects on liver wounds and shows good histocompatibility.

16.
Article in Chinese | WPRIM | ID: wpr-323537

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the risk of postoperative complications in elderly colorectal cancer patients over 65 years with comorbid cardiovascular diseases.</p><p><b>METHODS</b>A total of 381 elderly colorectal cancer patients over 65 years were pathologically diagnosed as colorectal adenocarcinoma and underwent the first surgery in Beijing Hospital during January 2013 and December 2014. Patients were divided into comorbid cardiovascular disease group (258 cases) and non-cardiovascular disease group (123 cases) according to the existence of comorbid cardiovascular disease. The morbidity of postoperative complication was compared between two groups.</p><p><b>RESULTS</b>There was no significant difference in the morbidity of postoperative complication between two groups [27.9%(72/258) vs. 29.3%(36/123), P>0.05]. According to the Clavien-Dindo classification of postoperative complications, the morbidities of complication at all levels between two groups were not significantly different(all P>0.05). But in terms of cardiovascular complications, the morbidity of comorbid cardiovascular disease group was significantly higher than that of non-cardiovascular disease group [7.4%(19/258) vs. 0.8%(1/123), χ=6.678, P=0.010], while no significant differences in pulmonary and abdominal complications were found between two groups(all P>0.05). The morbidities of other complications (deep vein thrombosis, urinary tract infection and renal complications, etc.) of comorbid cardiovascular disease group were lower than those in non-cardiovascular disease group [2.7%(7/258) vs. 8.1%(10/123), χ=5.733, P=0.017]. Different types of cardiovascular diseases, different levels of cardiac risk index and American Society of Anesthesiologists(ASA) rating were not significantly related to the patient's occurrence of postoperative complications(all P>0.05).</p><p><b>CONCLUSIONS</b>Surgery treatment for elderly colorectal cancer patients over 65 years with comorbid cardiovascular diseases is safe. However, strict cardiovascular monitoring should be performed and necessary measures should be carried out in time.</p>


Subject(s)
Adenocarcinoma , General Surgery , Aged , Aged, 80 and over , Cardiovascular Diseases , Colorectal Neoplasms , General Surgery , Comorbidity , Digestive System Surgical Procedures , Female , Humans , Male , Postoperative Complications , Epidemiology , Risk Factors
17.
Article in Chinese | WPRIM | ID: wpr-340621

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of phosphoinositide 4-phosphate (PI4P) on human glioma U87 cells and the mechanism of action of PI4P in the development of human glioma through the overexpression or silencing of PI4P in human glioma U87 cells, and to provide a new target for basic research and clinical treatment of glioma.</p><p><b>METHODS</b>LV-Helper1, LV-Helper2, pWPXLd-PI4P, and pLL3.7-shPI4P were used to package pWPXLd-PI4P and pLL3.7-shPI4P lentiviruses. The U87-GFP (PI4P-overexpression control group), U87-GFP-PI4P (PI4P-overexpression experimental group), U87-Scramble (PI4P-silencing control group), and U87-shPI4P (PI4P-silencing experimental group) cell lines were established. Wound-healing assay and Transwell assay were used to evaluate cell migration and invasion, and Western blot was used to measure the expression of PI4P in each group.</p><p><b>RESULTS</b>Western blot detected the expression of exogenous PI4P in the U87-GFP-PI4P cell line, and the U87-shPI4P cell line showed reduced expression of PI4P compared with the U87-Scramble cell line in the control group. The U87-GFP-PI4P cell line with PI4P overexpression had a significantly stronger ability of migration than the U87-GFP cell line in the control group (P<0.01); the U87-shPI4P cell line with PI4P silencing had a reduced ability of migration than the U87-Scramble cell line in the control group (P<0.01). The U87 cell line with PI4P overexpression had a significantly stronger invasion ability than the control group (P<0.05); after PI4P silencing, the experimental group showed a significant reduction in invasion ability compared with the control group (P<0.05).</p><p><b>CONCLUSIONS</b>In human glioma U87 cells, PI4P can promote the invasion and migration of glioma cells and may become a new target in the basic research and clinical treatment of glioma.</p>


Subject(s)
Cell Line, Tumor , Cell Movement , Glioma , Pathology , Humans , Neoplasm Invasiveness , Phosphatidylinositol Phosphates , Pharmacology
18.
Article in Chinese | WPRIM | ID: wpr-500847

ABSTRACT

Objective To evaluate the surgical modalities and prognosis in elderly patients with gastric cancer.Methods Sixty three gastric cancer patients aged ≥≥ 75 years underwent radical surgery,with D0 + D1 resection in 32 cases and D2 resection in 31 cases,in Beijing Hospital from January 2005 to December 2009.Results More lymph nodes were dissected in D2 group than those in D0 + D1 group (27.42 ± 12.75 vs.14.59 ± 12.11,t =-4.095,P < 0.05).There was no significant difference in postoperative complication rate [25.81% (8/31) vs.31.25% (10/32),x2 =0.095,P > 0.05] and perioperative death rate [3.23% (1/31) vs.6.25% (2/32),x2 =0.324,P > 0.05] between two groups.The 5-year survival rate was higher in D2 group than that in D0 ± D1 group (47.3% vs.21.4%,x2 =6.346,P < 0.05).The 5-year survival rate was higher in POSSUM score (PS) < 20 group than that in PS≥20 group (46.3% vs.11.1%,x2 =5.492,P <0.05).In PS≥20 group,postoperative complication rate did not increase after D2 radical resection(1/8 vs.3/10,x2 =0.824,P > 0.05).Univariate analysis showed that PS < 20 was associated with the prognosis of patients (x2 =5.492,P < 0.05).Cox proportional hazards model showed that lymph node metastasis (OR=4.103,95% CI:1.790-9.405,P < 0.05) and D2 radical gastrectomy(OR =0.313,95% CI:0.158-0.620,P < 0.05) were the independent factors associated with the prognosis of patients aged ≥ 75 years with gastric cancer (all P < 0.05).Conclusion Standardized D2 lymph node dissection is beneficial for gastric cancer patients aged ≥75 years.PS < 20,lymph node metastasis and D2 radical gastrectomy are associated with the prognosis of patients.

19.
Journal of Clinical Pediatrics ; (12): 876-879, 2015.
Article in Chinese | WPRIM | ID: wpr-477539

ABSTRACT

ObjectiveTo explore the role of the lymphocyte subsets in the peripheral blood in diagnosis, treatment and prognosis of hemophagocytic lymphohistiocytosis (HLH) in children.MethodA total of 30 children with HLH were enrolled in this study and treated according to the HLH-2004 diagnostic guidelines. 20 children with HLH entered complete remission (CR) and 10 children with HLH died. Thirty age-matched healthy children were selected as normal controls. T cell subsets in the pe-ripheral blood were measured by lfow cytometry.ResultsCompared with control group, CD3+T and CD8+T cells were signiif-cantly increased, CD4+T and CD3-CDl6+CD56+ NK cells were signiifcantly decreased, and CD4+/CD8+ cell ratio was signiifcantly decreased in 20 CR children and 10 died children with HLH in acute phase (P0.05). In acute phase, the lymphocyte subsets were not statistically different between 20 CR children and 10 died children (P>0.05). In 20 CR children, the proportion of CD3-CD16+CD56+NK in CR phase was statistically different than that in acute phase (P<0.05).ConclusionsChildren with HLH have obvious changes in peripheral blood lymphocyte subsets and have cellular immunity disorders. Dynamic detection of the changes may help determine the therapeutic effect and prognosis of HLH.

20.
Article in Chinese | WPRIM | ID: wpr-330258

ABSTRACT

Quantitative models were established to analyze the content of chlorogenic acid and soluble solid content in the liquid-liquid extraction of Reduning injection by near-infrared (NIR) spectroscopy. Seven batches of extraction solution from the liquid-liquid extraction of Lonicerae Japonicae Flos and Artemisiae Annuae Herba were collected and NIR off-line spectra were acquired. The content of chlorogenic acid and soluble solid content were determined by the reference methods. The partial least square (PLS) and artificial neural networks (ANN) were used to build models to predict the content of chlorogenic acid and soluble solid content in the unknown samples. For PLS models, the R2 of calibration set were 0.9872, 0.9812, RMSEC were 0.1533, 0.7943, the R2 of prediction set were 0.9837, 0.9733, RMSEP were 0.2464, 1.2594, RSEP were 3.25%, 3.31%, for chlorogenic acid and soluble solid content, respectively. For ANN models, the R2 of calibration set were 0.9903, 0.9882, RMSEC were 0.0974, 0.4543, the R2 of prediction set were 0.9868, 0.9699, RMSEP were 0.1920, 0.9427, RSEP were 2.61%, 2.75%, for chlorogenic acid and soluble solid content, respectively. Both the RSEP values of chlorogenic acid and soluble solid content were lower than 6%, which can satisfy the quality control standard in the traditional Chinese medicine production process. The RSEP values of ANN models were lower than PLS models, which indicated the ANN models had better predictive performance for chlorogenic acid and soluble solid content. The established method can rapidly measure the content of chlorogenic acid and soluble solid content. The method is simple, accurate anc reliable, thus can be used for quality control of the liquid-liquid extraction of Reduning injection.


Subject(s)
Chlorogenic Acid , Drugs, Chinese Herbal , Injections , Least-Squares Analysis , Liquid-Liquid Extraction , Reference Standards , Neural Networks, Computer , Quality Control , Spectroscopy, Near-Infrared , Methods
SELECTION OF CITATIONS
SEARCH DETAIL