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1.
Article in English | WPRIM | ID: wpr-888672

ABSTRACT

Although there is guidance from different regulatory agencies, there are opportunities to bring greater consistency and stronger applicability to address the practical issues of establishing and operating a data monitoring committee (DMC) for clinical studies of Chinese medicine. We names it as a Chinese Medicine Data Monitoring Committee (CMDMC). A panel composed of clinical and statistical experts shared their experience and thoughts on the important aspects of CMDMCs. Subsequently, a community standard on CMDMCs (T/CACM 1323-2019) was issued by the China Association of Chinese Medicine on September 12, 2019. This paper summarizes the key content of this standard to help the sponsors of clinical studies establish and operate CMDMCs, which will further develop the scientific integrity and quality of clinical studies.

4.
Article in Chinese | WPRIM | ID: wpr-878786

ABSTRACT

Orthogonal experiments were used to optimize the process parameters of curcumin TPP-PEG-PCL nanomicelles; the particle size, electric potential and morphology under the electron microscope were systematically detected for the curcumin TPP-PEG-PCL nanomicelles; and the stability and in vitro release of the curcumin TPP-PEG-PCL nanomicelles were investigated. With DID fluorescent dye as the fluorescent probe, flow cytometry was used to study the uptake of nanomicelles by breast cancer cells, and laser confocal microscopy was used to study the mitochondrial targeting and lysosomal escape functions of nanomicelles. Under the same dosage conditions, the effect of curcumin TPP-PEG-PCL nanomicelles on promoting the apoptosis of breast cancer cells was evaluated. The optimal particle size of curcumin TPP-PEG-PCL nanomicelle was(17.3±0.3) nm, and the Zeta potential was(14.6±2.6) mV in orthogonal test. Under such conditions, the micelle appeared as regular spheres under the transmission electron microscope. Fluorescence test results showed that TPP-PEG-PCL nanomicelles can promote drug uptake by tumor cells, escape from lysosomal phagocytosis, and target the mitochondria. The cell survival rate and Hoechst staining positive test results showed that curcumin TPP-PEG-PCL nanomicelles had a good effect on promoting apoptosis of breast cancer cells. The curcumin TPP-PEG-PCL micelles can significantly reduce the mitochondrial membrane potential of breast cancer cells, increase the release of cytochrome C, significantly increase the expression of pro-apoptotic protein Bcl-2 and reduce the expression of anti-apoptotic Bax protein. These test results were significantly better than those of curcumin PEG-PCL nanomicelles and curcumin, with statistically significant differences. The results revealed that curcumin TPP-PEG-PCL nanomicelles can well target breast cancer cell mitochondria and escape from the lysosomal capture, thereby enhancing the drug's role in promoting tumor cell apoptosis.


Subject(s)
Apoptosis , Breast Neoplasms/drug therapy , Cell Line, Tumor , Curcumin/pharmacology , Humans , Lysosomes , Micelles , Mitochondria , Phosphatidylethanolamines , Polyethylene Glycols
5.
Article in Chinese | WPRIM | ID: wpr-816067

ABSTRACT

Congenital heart disease(CHD)is one of the most common cardiovascular diseases in children, which ranks the first place of birth defects and severely threatens human health. With the developments of medical apparatus and progress of medical technology,interventional therapy has already become the preferred therapeutic choice for certain types of CHD including atrial septal defect,ventricular septal defect and patent ductus arteriosus, etc. However, occluders currently used are made of NiTi, which exist permanently in human body after implantation. Generally, the surface endothelialization of occluders makes them lose its significance of existence 6 months after implantation. Ideal occluders should gradually undergo self-degradation till they vanish, thus avoiding the permanent existence of metal materials. Nowadays, with the development of medical materials, such an idea has become possible.

6.
Tianjin Medical Journal ; (12): 1095-1098, 2017.
Article in Chinese | WPRIM | ID: wpr-660269

ABSTRACT

Objective To analyze the epidemiological characteristics and the change trends of malignant tumor death in Xiqing District of Tianjin in 2010-2015, and to provide reference for prevention and treatment of malignant tumor. Methods The death monitoring data in 2010-2015 of Xiqing District were collected, and which were classified by international classification of diseases ICD-10 encoding. The mortality rate, standardized mortality rate of malignant tumor and tumor death in different gender and age groups were analyzed. Results The average annual crude mortality rate of malignant tumors was 142.90/100000 in Xiqing District of Tianjin from 2010 to 2015, and the standardized mortality rate was 98.95/100000. The average crude mortality rate of male was 165.25/100000, the standardized mortality rate was 110.21/100000. The average crude mortality rate of female was 121.23/100000, and the standardized mortality rate was 87.54/100000. Among the leading causes of death in malignant tumors, the top three leading cancer types were lung cancer (62.42/100000), liver cancer (18.82/100000) and gastric cancer (13.22/100000). The mortality rates of lung cancer, liver cancer, gastric cancer, colorectal cancer, leukemia, esophageal cancer, bladder cancer and nasopharyngeal carcinoma were significantly higher in males than those in females (P<0.05). There were death cases in different age groups, and the high mortality rate was concentrated in the age group of 0-1 and age group of more than 85. Conclusion The lung cancer, liver cancer, gastric cancer and other malignant tumors pose a serious threat to the health of Xiqing district residents. It is important to improve the monitoring system and promote the prevention and control of risk factors, continue to strengthen tobacco control and air pollution control, advocate a reasonable diet, moderate exercise and healthy lifestyle, take the comprehensive intervention measures, and reduce the incidence of malignant tumors.

7.
Tianjin Medical Journal ; (12): 1095-1098, 2017.
Article in Chinese | WPRIM | ID: wpr-657816

ABSTRACT

Objective To analyze the epidemiological characteristics and the change trends of malignant tumor death in Xiqing District of Tianjin in 2010-2015, and to provide reference for prevention and treatment of malignant tumor. Methods The death monitoring data in 2010-2015 of Xiqing District were collected, and which were classified by international classification of diseases ICD-10 encoding. The mortality rate, standardized mortality rate of malignant tumor and tumor death in different gender and age groups were analyzed. Results The average annual crude mortality rate of malignant tumors was 142.90/100000 in Xiqing District of Tianjin from 2010 to 2015, and the standardized mortality rate was 98.95/100000. The average crude mortality rate of male was 165.25/100000, the standardized mortality rate was 110.21/100000. The average crude mortality rate of female was 121.23/100000, and the standardized mortality rate was 87.54/100000. Among the leading causes of death in malignant tumors, the top three leading cancer types were lung cancer (62.42/100000), liver cancer (18.82/100000) and gastric cancer (13.22/100000). The mortality rates of lung cancer, liver cancer, gastric cancer, colorectal cancer, leukemia, esophageal cancer, bladder cancer and nasopharyngeal carcinoma were significantly higher in males than those in females (P<0.05). There were death cases in different age groups, and the high mortality rate was concentrated in the age group of 0-1 and age group of more than 85. Conclusion The lung cancer, liver cancer, gastric cancer and other malignant tumors pose a serious threat to the health of Xiqing district residents. It is important to improve the monitoring system and promote the prevention and control of risk factors, continue to strengthen tobacco control and air pollution control, advocate a reasonable diet, moderate exercise and healthy lifestyle, take the comprehensive intervention measures, and reduce the incidence of malignant tumors.

8.
Chinese Medical Journal ; (24): 3456-3459, 2013.
Article in English | WPRIM | ID: wpr-354454

ABSTRACT

<p><b>BACKGROUND</b>Occluders licensed for clinical use are not fit for some special Krichenko E patent ductus arterioses. The Amplatzer vascular plug I (AVP1) has not been licensed for use for closure of patent ductus arteriose. We report our initial experience to occluding special type patent ductus arterioses with the AVP1-a single lobe device of single layer Nitinol mesh for short vessel landing zones.</p><p><b>METHODS</b>Patients referred with small and long Krichenko E patent ductus arterioses 1 mm to 3 mm in diameter underwent occlusion using AVP1. All cases underwent pre-, intra- and post-procedural echocardiography and chest X-ray at the completion of the procedure, the next day and at a 30-day, 3-month and 6-month follow-up visits. Device sizing for device waist diameter and length was based on aortography.</p><p><b>RESULTS</b>From April 2008 to June 2012, 26 patients with a mean age of (7.6 ± 8.0) years (range 6 months-32 years) and a mean weight of (23.8 ± 14.8) kg (range 7-67 kg) underwent successful patent ductus arteriose closure. The mean ductus diameter was (2.1 ± 0.7) mm (range 1-3 mm). Transpulmonary (22/26) and transaortic approaches (4/26) were used. No persistent patency was observed after 24 hours and after one month. No device displacement, residual flow and iatrogenic coarctation of the aorta were observed after three months and six months.</p><p><b>CONCLUSIONS</b>The AVP1 makes it easy to close some Krichenko E patent ductus arterioses. Smaller delivery catheter profile and symmetric cylindrical device shape allow for use for small and long Krichenko E patent ductus arterioses 1 mm to 3 mm in diameter and small patients through transaortic approaches. Broader experience is required to further delineate device and patient selection as well as to document its long-term efficacy and safety.</p>


Subject(s)
Adolescent , Adult , Child , Child, Preschool , Ductus Arteriosus, Patent , General Surgery , Female , Humans , Infant , Male , Septal Occluder Device , Young Adult
9.
Article in Chinese | WPRIM | ID: wpr-267589

ABSTRACT

<p><b>OBJECTIVE</b>To establish a rabbit model of intervertebral disc degeneration by puncturing the anulus fibrosus through an approach between the longissimus dorsi muscle and obliquus externus abdominis.</p><p><b>METHODS</b>The L(4/5) and L(5/6) intervetebral discs of 6 New Zealand white rabbits were punctured by an 18-gauge pin in the anterolateral annular fibrosus through an approach between the longissimus dorsi muscle and the obliquus externus abdominis with the right transverse processes of L(5) and L(6) resected; the L(2/3) discs were used as the control without exposure or needle stab, and the L(3/4) discs were subjected to sham operation with the discs exposed but not punctured after resecting the right transverse process of L(4). X-ray and magnetic resonance imaging (MRI) were performed preoperatively and at the 4th week after puncture. At 4 weeks after the operation, histological and immunohistochemical analyses of the discs were carried out.</p><p><b>RESULTS</b>X-ray of the punctured discs at 4 weeks after the operation presented a significant decrease of disc height, osteophytosis formation, and end-plate stiffness; an obvious decrease of signal intensity on T(2)-weighted images was found in the puncture group but not in the control or sham-operated groups. Gross morphological inspection showed atrophy of the nucleus pulposus, which became loose, soft, and fragile with a light yellow color. Histological and immunohistochemical analyses showed a significant decrease of notochordal cells and type II collagen in the nucleus pulposus in the puncture group as compared to the control and sham-operated groups.</p><p><b>CONCLUSION</b>Puncture through the approach between the longissimus dorsi muscle and the obliquus externus abdominis allows the establishment of a reliable animal model for studying intervertebral disc degeneration.</p>


Subject(s)
Animals , Disease Models, Animal , Female , Intervertebral Disc Degeneration , Lumbar Vertebrae , Male , Rabbits
10.
Chinese Journal of Cardiology ; (12): 909-914, 2011.
Article in Chinese | WPRIM | ID: wpr-268287

ABSTRACT

<p><b>OBJECTIVE</b>To screen the cardiac troponin T (TNNT2) mutations in Chinese patients with hypertrophic cardiomyopathy (HCM) and to analyze the potential link between the genotype and the phenotype.</p><p><b>METHODS</b>Clinical features of 100 probands with HCM and some family members were evaluated, 200 unrelated normal subjects served as control. The exons and flanking introns of TNNT2 were amplified with PCR and direct sequencing was used to screen TNNT2 mutations/polymorphisms.</p><p><b>RESULTS</b>Two novel missense mutations were detected in 2 HCM patients: R92W and R286H. These 2 mutations were not found in 200 non-HCM controls. A five-basepair insertion/deletion polymorphism in intron 3 of TNNT2 was identified in this HCM cohort but was not related to the phenotype.</p><p><b>CONCLUSIONS</b>Two missense mutations, R92W and R286H, were found in 2/100 patients with HCM, TNNT 2 mutation is relatively low in Chinese patients with HCM.</p>


Subject(s)
Asian Continental Ancestry Group , Cardiomyopathy, Hypertrophic , Genetics , Case-Control Studies , Exons , Genotype , Humans , Mutation , Mutation, Missense , Pedigree , Phenotype , Polymorphism, Genetic , Troponin T , Genetics
11.
Chinese Journal of Cardiology ; (12): 993-996, 2011.
Article in Chinese | WPRIM | ID: wpr-268270

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the clinical feature of patients with atrial septal defects (ASD) and the safety and efficacy of transcatheter closure of ASD in elderly patients.</p><p><b>METHODS</b>Between May 2000 and June 2010, 82 patients aged (64.5 ± 3.8) years underwent attempted transcatheter ASD closure. Right heart catheterization was performed before intervention. Echocardiography was made at 1 day, 1, 3, 6 months after the procedure. The pre- and post-closure clinical feature, pulmonary artery pressure (PAP) and cardiac function were evaluated.</p><p><b>RESULTS</b>In 82 patients, 37 (45.1%) patients were associated with pulmonary arterial hypertension (PAH). The systolic PAP and mean PAP [(44.1 ± 12.4) mm Hg (1 mm Hg = 0.133 kPa) and (25.2 ± 6.8) mm Hg, respectively] were measured by right heart catheterization before the procedure. One patient was unsuitable for closure because of severe PAH. The remaining 81 patients underwent successful ASD closure without major complications. After closuring, systolic PAP decreased from (52.7 ± 10.3) mm Hg to (31.8 ± 6.3) mm Hg (P < 0.05), and mean PAP descended from (30.9 ± 4.7) mm Hg to (21.8 ± 3.4) mm Hg (P < 0.05) in the 36 patients with PAH. The cardiac function improved post procedure. There were 6 new-onset atrial fibrillations during follow up.</p><p><b>CONCLUSIONS</b>ASD in elderly patients are commonly associated with PAH. Transcatheter ASD closure is safe and effective in the majority of elderly patients.</p>


Subject(s)
Aged , Cardiac Catheterization , Female , Heart Septal Defects, Atrial , General Surgery , Humans , Male , Middle Aged , Treatment Outcome
12.
Article in Chinese | WPRIM | ID: wpr-291553

ABSTRACT

<p><b>OBJECTIVE</b>To establish a non-parametric probabilistic model for evaluation of Chinese dietary exposure and to improve the assessment accuracy while integrating into the global risk assessment on food safety.</p><p><b>METHODS</b>Contamination data was from the national food contamination monitoring program during 2000 - 2006, including heavy metals, pesticides and mycotoxins, amounting to 135 contaminants with 499 commodities and 487 819 samples. Food consumption data was obtained from the national diet and nutrition survey conducted in 2002 with three consecutive days by 24-hour recall method, and 66 172 consumers were included. Monte Carlo simulation was applied to derive the intake distribution, and the uncertainty of each percentile was estimated using the Bootstrap sampling.</p><p><b>RESULTS</b>Different non-parametric probabilistic models for dietary exposure evaluation on heavy metals, pesticides and some of the toxins were established for Chinese people, and intake distributions with 95% confidence intervals of these contaminants were estimated. Taking acephate as an example, the results of its model shows that, for the 7 - 10 year-old children, the median dietary exposure in urban and rural areas were 1.77 microg x kg(-1) x d(-1) and 2.48 microg x kg(-1) x d(-1) respectively, with a 95% confidence interval of (1.59 - 2.06) microg x kg(-1) x d(-1) and (2.33 - 2.80) microg x kg(-1) x d(-1) respectively.</p><p><b>CONCLUSION</b>The non-parametric probabilistic model can quantify the variability and uncertainty of exposure assessment and improve the assessment accuracy.</p>


Subject(s)
China , Consumer Product Safety , Diet Surveys , Humans , Models, Statistical , Risk Assessment , Statistics, Nonparametric
13.
Chinese Medical Journal ; (24): 555-558, 2010.
Article in English | WPRIM | ID: wpr-314545

ABSTRACT

<p><b>BACKGROUND</b>The second-trimester maternal serum screening in twin pregnancy is still controversial, as the serum marker levels in twins are not as clear as those in singletons. This study aimed to evaluate the relationship between the levels of the second-trimester maternal serum free beta-human chorionic gonadotropin (free beta-HCG) and alpha-fetoprotein (AFP) in normal twin and singleton pregnancies and to estimate feasible analysis methods for utilizing these markers in second trimester screening for twin pregnancy.</p><p><b>METHODS</b>On the basis of a prospective population-based study of second-trimester maternal serum screening, the concentrations of maternal serum AFP and free beta-HCG of 195 normal twin pregnancy and 26,512 singleton controls at gestational weeks 15 to 20 were measured by time-resolved fluoroimmunoassay in one laboratory. The levels of markers were compared between the twins and singletons using weight-correction and gestational age-specific model.</p><p><b>RESULTS</b>According to the research protocol, 95 communities were randomly sampled, which covered the whole Jiangsu province, the east of China. A total of 26 803 pregnant women (98%), from the target population accepted prenatal screening for maternal serum AFP, beta-HCG detection, and all babies were followed up for at least six months. There were 197 (0.73%) twin pregnancies, of which one case had fetal trisomy 18, and one case with fetal anencephaly. The others were normal twin pregnancy. From a total enrollment of 26 803 women participants, 26 512 women with normal singleton pregnancies were selected as the model controls. The other 291 pregnancies, including trisomy 21, neural tube defect (NTD), trisomy 18, and other fetal abnormalities, were excluded. No significant differences were found in the medians of gestational age-specific maternal serum free beta-hCG and AFP in normal twin pregnancy comparing with twice those in model controls with the exception of the medians for free beta-hCG during the 16th gestational week (P = 0.012).</p><p><b>CONCLUSION</b>The weight-correction and gestational age-specific levels of Chinese Han population maternal serum free beta-hCG and AFP in normal twins were twice the levels as those in the singleton controls during the 17-19 gestational weeks.</p>


Subject(s)
Adult , Chorionic Gonadotropin, beta Subunit, Human , Blood , Female , Humans , Pregnancy , Blood , Pregnancy Trimester, Second , Twins , alpha-Fetoproteins
14.
Chinese Journal of Oncology ; (12): 490-494, 2009.
Article in Chinese | WPRIM | ID: wpr-293082

ABSTRACT

<p><b>OBJECTIVE</b>To study the anti-tumor effects of Newcastle disease virus (NDV) strain D817 on human colon carcinoma model in nude mice.</p><p><b>METHODS</b>The nude mouse model of human colon carcinoma was established by subcutaneous inoculation of human colon cancer LOVO cells. The tumor-bearing mice were given PBS, 5-Fu, high-dose NDV D817, moderate-dose NDV D817 or low-dose NDV D817 via caudal vein injection. The tumor size and weight of mice were measured. The liver damages were examined by histopathology. Apoptosis and necrosis of tumor cells were detected by flow cytometry. The endotumoral content of TNF-alpha was detected using a mouse TNF-alpha ELISA kit. The live virus was detected by hemagglutination (HA) test.</p><p><b>RESULTS</b>The moderate-dose NDV D817 inhibited the tumor growth more apparently than 5-Fu. The tumor growth inhibition rate reached to 48.1%. The liver damage and the weight change caused by NDV were less severe. NDV D817 made an increased apoptosis index and induced production of TNF-alpha. Live virus was not detected in important organs except in the tumor of nude mice by HA test.</p><p><b>CONCLUSION</b>In the anti-tumor process in nude mice bearing xenografts of human colon carcinoma, a suitable dose of NDV D817 is more safe and effective.</p>


Subject(s)
Animals , Apoptosis , Cell Line, Tumor , Colonic Neoplasms , Pathology , Therapeutics , Humans , Liver , Pathology , Mice , Mice, Inbred BALB C , Mice, Nude , Newcastle disease virus , Physiology , Random Allocation , Tumor Burden , Tumor Necrosis Factor-alpha , Metabolism , Xenograft Model Antitumor Assays
15.
Chinese Journal of Cardiology ; (12): 998-1000, 2009.
Article in Chinese | WPRIM | ID: wpr-323952

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the clinical feature and the effects of transcatheter closure of adult patients with patent ductus arteriosus (PDA).</p><p><b>METHODS</b>Between January 2000 and April 2009, 139 patients [22 male, aged from 40 to 74: (49.8 +/- 6.8) years] with PDA were hospitalized in our hospital. Clinical data and effects of transcatheter closure of PDA were analyzed.</p><p><b>RESULTS</b>There were 64 patients with NYHA classI, 53 with class II, 16 with class III and 6 with class IV before procedure. In 139 patients, pulmonary arterial hypertension (PAH) was found in 107 out of 139 patients (77.0%). Transcatheter PDA closure was not performed in 3 patients due to severe PAH and successfully performed in the remaining 136 patients (97.8%) without major complications. Post procedure aortic angiography evidenced minor residual shunt in 14 cases, small residual shunt in 2 cases and moderate shunt in 1 case. The NYHA class was significantly improved and the PAH significantly reduced [sPAP: (47.3 +/- 23.9) mm Hg (1 mm Hg = 0.133 kPa) vs. (28.1 +/- 12.3) mm Hg, P < 0.01] post procedure.</p><p><b>CONCLUSION</b>PAH and heart failure were commonly associated with PDA in adult patients. Transcatheter PDA closure is safe and effective in these patients except those with severe PAH.</p>


Subject(s)
Adult , Aged , Cardiac Catheterization , Ductus Arteriosus, Patent , Therapeutics , Female , Humans , Hypertension, Pulmonary , Male , Middle Aged , Severity of Illness Index , Treatment Outcome
16.
Chinese Journal of Surgery ; (12): 187-189, 2009.
Article in Chinese | WPRIM | ID: wpr-238930

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the significance of three dimensional visualization and virtual surgery system in living related donor liver transplantation surgery.</p><p><b>METHODS</b>Two patients suffered biliary calculi were scanned by 64 slice helical computer tomography (CT) on livers and the data were imported into medical image proceeding system (MIPS) for sequence. Man-made segmentation and true-up on the image from the data were carried out. Three dimensional (3D) models of the liver and the intrahepatic vessels were reconstructed by VTK software respectively. The models were exported with format STL from it and then were imported into the FreeForm Modeling System for smoothing and modifying. At last, living related donor liver transplantation were simulated with the force-feedback equipment (PHANToM).</p><p><b>RESULTS</b>It had great verisimilar image for the reconstructed 3D liver models with artery, hepatic vein, portal vein and bile duct. By seeing through liver, it had high fidelity and strong 3D effect for the intrahepatic artery, hepatic vein, portal vein and bile duct, and their spatial disposition and course and co-relationship were shown clearly. In the virtual surgery system, the virtual scalpel could be manipulated on 3D liver model with PHANToM. The simulating effect was the same as the clinic operation for living related donor liver transplantation.</p><p><b>CONCLUSIONS</b>The visualized liver model reconstructed is 3D and verisimilar, and it is helpful to design reasonable scheme for liver transplantation. It can improve the surgical effect, decrease the surgical risk, reduce the complication, enhance the communication between doctor and patient through designing surgical plan and demonstrating visualized operation before surgery.</p>


Subject(s)
Adult , Computer Simulation , Female , Humans , Image Processing, Computer-Assisted , Imaging, Three-Dimensional , Liver , Diagnostic Imaging , Liver Transplantation , Living Donors , Models, Anatomic , Tomography, Spiral Computed , User-Computer Interface
17.
Chinese Journal of Cardiology ; (12): 734-738, 2009.
Article in Chinese | WPRIM | ID: wpr-236415

ABSTRACT

<p><b>OBJECTIVE</b>To screen the MYBPC3 gene mutations in Han Chinese patients with hypertrophic cardiomyopathy (HCM).</p><p><b>METHODS</b>Sixty-six patients with HCM were enrolled for the study. The exons in the functional regions of MYBPC3 were amplified with PCR and the products were sequenced.</p><p><b>RESULTS</b>Four novel mutations and four common polymorphisms were identified in this patient cohort. A Lys301fs mutation in exon10 was evidenced in a H30, and when he was 47 years old, he had the chest tightness, shortness of breath with septal hypertrophy of 18.7mm; a Asp463stop mutation in exon17 was detected in a H48, he was 24 years old 24-year-old when a medical examination showed ventricular septal hypertrophy of 15.4 mm; both Gly523Arg mutation in exon18 and Tyr847His mutation in exon26 were found in a H53 with onset age 36 years old, feeling chest tightness after excise and his ventricular septal hypertrophy was 27 mm that time. MYBPC3 mutations occurred in 4.5% patients in this cohort. These mutations were not found in 100 non-HCM control patients.</p><p><b>CONCLUSION</b>MYBPC3 mutation is presented in a small portion of Han Chinese patients with HCM.</p>


Subject(s)
Adult , Asian Continental Ancestry Group , Genetics , Cardiomyopathy, Hypertrophic , Genetics , Carrier Proteins , Genetics , DNA Mutational Analysis , Exons , Female , Genotype , Humans , Male , Middle Aged , Mutation , Phenotype , RNA, Messenger , Genetics
18.
Article in Chinese | WPRIM | ID: wpr-339078

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the value of virtual surgery in hepatic artery reconstruction in liver recipients with type II hepatic artery variation.</p><p><b>METHODS</b>A patient with cholangiocellular carcinoma and a healthy individual were scanned using 64-slice spiral CT, and image segmentation and three-dimensional (3D) reconstruction were performed using an image processing system. The 3D models in STL format were then imported to the FreeForm Modeling System for smoothing and refinement. Hepatic artery reconstruction was performed in simulated liver transplantation using the virtual surgery system with force feedback (PHANTOM).</p><p><b>RESULTS</b>The reconstructed model contained the liver, hepatic arteries, biliary system, and bile duct tumor emboli and displayed the entire branching of the hepatic artery with type II variation. Using the virtual surgery system, arterial reconstruction was performed by anastomosing the donor celiac trunk and the recipient abdominal aorta with the virtual scalpel and needle.</p><p><b>CONCLUSION</b>The reconstructed model allows clearer views of the 3D structures of the arteries in the liver and helps in preoperative preparations and surgical planning of artery reconstruction during liver transplantation. This approach may also help reduce the surgical risks and potential complications.</p>


Subject(s)
Computer Simulation , Computer-Assisted Instruction , Methods , Female , Hepatic Artery , Congenital Abnormalities , General Surgery , Humans , Imaging, Three-Dimensional , Methods , Liver Neoplasms , Diagnostic Imaging , Pathology , General Surgery , Liver Transplantation , Middle Aged , Surgery, Computer-Assisted , Methods , Tomography, Spiral Computed
19.
Chinese Journal of Cardiology ; (12): 633-636, 2007.
Article in Chinese | WPRIM | ID: wpr-307231

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the risk factors for early arrhythmias after transcatheter closure of perimembranous ventricular septal defect (PVSD).</p><p><b>METHODS</b>A total of 358 patients [161 males, aged from 3 to 54, mean (10.9 +/- 8.1) years, body weight from 12 to 90, mean (32.8 +/- 17.2) kg] who underwent transcatheter closure of PVSD from August 2002 to February 2006 were included in this retrospective analysis. Electrocardiogram was performed daily after transcatheter closure for seven days. Relationships between arrhythmias and those risk factors such as the defect characteristics and the device size and types were explored by logistic regression analysis. Left ventriculography showed 195 out of 358 patients with PVSD were complicated with membranous aneurysm. The PVSD diameter ranged from 2 to 18 (6.5 +/- 3.1) mm in left ventricular side and from 2 to 12 (4.2 +/- 2.3) mm in right ventricular side. A total of 140 nonsymmetrical and 218 symmetrical occluders with diameter 4 to 18 (8.1 +/- 2.5) mm were used to close those defects.</p><p><b>RESULTS</b>Procedure was successful in all patients. Early arrhythmias after transcatheter closure of PVSD were observed in 135 (37.7%) patients and serious cardiac arrhythmias in 23 (6.4%) patients. The early arrhythmias after transcatheter closure of PVSD were significantly correlated with device size [> or = (8.6 +/- 2.7) mm] and type (nonsymmetrical device), the span between the defect and tricuspid (< or = 3 mm), and the presence of aneurysm.</p><p><b>CONCLUSION</b>Larger device size, nonsymmetrical device, narrow span between the defect and tricuspid and the presence of aneurysm are the risk factors for early arrhythmias after transcatheter closure of PVSD.</p>


Subject(s)
Adolescent , Adult , Arrhythmias, Cardiac , Cardiac Catheterization , Child , Child, Preschool , Female , Heart Septal Defects, Ventricular , Therapeutics , Humans , Male , Middle Aged , Postoperative Complications , Retrospective Studies , Risk Factors , Young Adult
20.
Chinese Journal of Cardiology ; (12): 202-207, 2006.
Article in Chinese | WPRIM | ID: wpr-295346

ABSTRACT

<p><b>OBJECTIVE</b>The aim of this study was to screen the disease-causing gene mutations and investigate the genotype-phenotype correlation in 10 Chinese pedigrees with familial hypertrophic cardiomyopathy (HCM).</p><p><b>METHODS</b>There are 91 family members from these 10 pedigrees and 5 members were normal mutated carriers, 23 members were HCM patients (14 male) aged from 1.5 to 73 years old. The functional regions of myosin heavy chain gene (MYH7), cardiac myosin-binding protein C (MYBPC3) and cardiac troponin T gene (TNNT2) were screened with PCR and direct sequencing technique. Clinical information from all patients was also evaluated in regard to the genotype.</p><p><b>RESULTS</b>Mutations were found in 5 out of 10 pedigrees. Mutations in MYH7 (Arg663His, Glu924Lys and Ile736Thr) were found in 3 pedigrees and 3 patients from these pedigrees suffered sudden death at age 20-48 years old during sport. Mutations in MYBPC3 were found in 2 pedigrees, 1 with complex mutation (Arg502Trp and splicing mutation IVS27 + 12C > T) and 1 with novel frame shift mutation (Gly347fs) and the latter pedigree has sudden death history. No mutation was identified in TNNT2.</p><p><b>CONCLUSIONS</b>Although the Han Chinese is a relatively homogeneous ethnic group, different HCM gene mutations were responsible for familiar HCM suggesting the heterogeneity nature of the disease-causing genes and HCM MYH7 mutations are associated with a higher risk of sudden death in this cohort. Furthermore, identical mutation might result in different phenotypes suggesting that multiple factors might be involved in the pathogenesis of familiar HCM.</p>


Subject(s)
Adolescent , Adult , Aged , Asian Continental Ancestry Group , Genetics , Cardiac Myosins , Genetics , Cardiomyopathy, Hypertrophic, Familial , Ethnology , Genetics , Carrier Proteins , Genetics , Child , Child, Preschool , Female , Humans , Infant , Male , Middle Aged , Mutation , Myosin Heavy Chains , Genetics , Pedigree , Phenotype , Troponin T , Genetics , Young Adult
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