Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 177
Filter
1.
Chinese Journal of Stomatology ; (12): 233-241, 2022.
Article in Chinese | WPRIM | ID: wpr-935856

ABSTRACT

Objective: To analyze the articles on research of dental pulp biology in China and to understand the situation of China in the entire field of dental pulp biology around the world in order to provide references for further in-depth research in dental pulp biology in China. Methods: Based on Web of Science core collection database, the articles published in the international journals in the field of dental pulp biology from 2011 to 2020 were retrieved and identified. Six indicators including research scale, academic influence, high-ranking and the high-influence journals, research areas, international partnership and project funding were statistically analyzed. Results: Totally 1 215 articles were published by Chinese researchers, which is the most in the research field of dental pulp biology. The total number of citations was 18 328, however the average number of citations of above mentioned articles was slightly lower than that of the world average. The number of articles published in Natural Index Journals and Journal of Dental Research is lower than that of the United States. The research areas of dental pulp biology in China were not only in dentistry, oral surgery and medicine, but also in cell biology, experimental medicine, materials science, engineering, molecular biology and applied microbiology. Articles of internationally cooperated researches were scarce. Most of the researches of dental pulp biology field conducted in China were supported by the National Natural Science Foundation of China. Conclusions: Although there were fruitful research outcomes in the field of dental pulp biology from 2011 to 2020, the academic influence of these researches still needed to be improved. It was recommended that great efforts should be made in developing interdisciplinary, inter-unit and international cooperation, focusing on hotspot and major projects, actively applying for and using of project fundings in order to produce more high-quality research outcomes.


Subject(s)
Bibliometrics , Biology , China , Dental Pulp , Periodicals as Topic
2.
International Eye Science ; (12): 1357-1360, 2022.
Article in Chinese | WPRIM | ID: wpr-935012

ABSTRACT

AIM:To discuss the efficacy and safety of orthokeratology with reducing back optic zone diameter(5mm-BOZD)compared with conventional back optic zone diameter(6mm-BOZD)in the treatment of adolescent myopia.METHODS: A prospective randomized controlled trial was performed. There were 100 cases with 100 eyes of adolescent myopia(all right eye data were taken)selected from April 2016 to January 2019, the spherical degree was -1.00--5.00D. Then they were randomly divided into the two groups. The experimental group wore 5mm-BOZD orthokeratology, and the control group wore 6mm-BOZD orthokeratology. Their axis length(AL), spherical equivalent(SE), relative peripheral refraction(RPR), best corrected visual acuity(BCVA), uncorrected near visual acuity(NVA), Efron grading was applied to record the anterior segment of the eyes, corneal hysteresis(CH), corneal resistance factor(CRF), corneal-compensated intraocular pressure(IOPcc), average noninvasive Keratograph tear breakup time(NIKBUTav)and higher order aberration(RMSh) were compared between the two groups during the 1a treatment period.RESULTS: After 1a of treatment, the AL in experimental group increased 0.05±0.05mm,while it increased 0.15±0.05mm(t=-8.949, P<0.001)in control group. The SE in experimental group increased -0.18±0.27D,while it increased-0.42±0.35D(t=3.609, P=0.001)in control group. There were statistical differences in RPR changes at N30°, N20° and T30°sites between the two groups(P<0.05). There were no statistical differences in BCVA,NVA,Efron grade,CH,CRF,IOPcc,NIKBUTav and RMSh between the two groups(P>0.05).CONCLUSION:Reducing back optic zone diameter orthokeratology can correct adolescent myopia safely and more effectively during the observation period.

3.
Article in Chinese | WPRIM | ID: wpr-931824

ABSTRACT

Objective:To assess the ability of the acute physiology and chronic health evaluation Ⅱ (APACHEⅡ) and trauma-injury severity score (TRISS) in predicting mortality in intensive care unit (ICU) trauma patients.Methods:Databases of PubMed, Cochrane Library, SinoMed, CNKI were retrieved from January 1980 to December 2020. The ability of the APACHE Ⅱ and the TRISS to predict mortality in the ICU trauma patients was compared in the retrieval literatures. The relevant literatures were screened by two researchers independently. The data of the included literatures were extracted, and the quality of the included literatures was evaluated. MetaDiSc 1.4 software was used to test the heterogeneity among studies. Meta-analysis was performed on diagnostic accuracy indicators and the summary receiver operator characteristics curve (SROC curve) was fitted. The area under SROC curve (AUC) of the two scores was compared. Deek test was used to analyze literature publication bias.Results:Six studies were selected with 4 054 patients involved with medium and high quality. Meta-analysis results showed that APACHE Ⅱ and TRISS had low sensitivity [the pooled sensitivity and 95% confidence interval (95% CI) was 0.48 (0.41-0.55) and 0.51 (0.41-0.62)], high specificity [the pooled specificity and 95% CI was 0.96 (0.93-0.97) and 0.98 (0.95-0.99)], the pooled diagnostic odds ratio ( DOR) and 95% CI was 20 (14-28) and 46 (18-120), and overall good performance in terms of AUC [the AUC and 95% CI was 0.79 (0.75-0.82) and 0.80 (0.76-0.83)] in predicting the prognosis of ICU trauma patients. There was no statistical difference in AUC between the two scores ( Z = 1.542, P > 0.05). Deek funnel plot showed little publication bias. Conclusion:Both APACHE Ⅱ and TRISS scores could accurately predict mortality in ICU trauma patients.

4.
Article in Chinese | WPRIM | ID: wpr-930984

ABSTRACT

Objective:To study the relationship between white matter injury (WMI) and brain maturity in preterm infants at full-term corrected gestational age (cGA).Methods:A retrospective study was performed in preterm infants [GA≤32 weeks or birth weight (BW) ≤1 500 g] admitted to the neonatal intensive care unit of the First Affiliated Hospital of Xi'an Jiaotong University from January 2017 to August 2018 and the Northwest Women and Children's Hospital from January 2017 to June 2017. The infants received conventional magnetic resonance imaging (MRI) at cGA 37~42 weeks. The infants were assigned into the WMI group and the control group according to the WMI scoring system, including the total maturation scores (TMS) and four sub-item scores.Results:A total of 118 premature infants were enrolled in this study (17 cases in the WMI group and 101 cases in the control group). The GA was (30.3±1.7) weeks, and BW was (1 356±268) g. The proportion of delayed TMS in the WMI group was significantly higher than the control group [58.8%(10/17) vs. 31.7%(32/101), P<0.05]. The TMS of the WMI group were significantly lower than the control group [(10.7±1.8) vs. (11.8±1.5), P<0.05]. The sub-item scores of TMS showed that the myelination [(2.8±0.6) vs. (3.1±0.4), P<0.05] and glial cell migration bands of the WMI group [(1.6±0.4) vs. (2.1±0.6), P=0.004] were significantly lower than the control group and no significant differences existed in cortical folding and involution of germinal matrix tissue scores between the two groups. Conclusions:The brain maturity of preterm infants with WMI is substantially delayed than those without WMI, including delayed myelination and delayed disappearance of glial cell migration bands.

5.
Acta Pharmaceutica Sinica ; (12): 605-614, 2022.
Article in Chinese | WPRIM | ID: wpr-922903

ABSTRACT

Proteasome controls the degradation of proteins closely related to life activities and plays a key role in the maintenance of protein homeostasis. Proteasome activities decrease with aging, followed by the overwhelming production of damaged proteins which far exceed the protein consumption. Accumulation of these proteins leads to various diseases including neurodegenerative diseases. Therefore, inducing toxic protein degradation is considered as a promising solution for the treatment of these diseases, while increasing the activity of proteasome is considered as an important strategy. However, the research in this field is still in the preliminary stage, and this review will focus on the discussion of the research progress of various small molecule proteasome activators, including research methods, pharmacological effects, structure-activity relationships and the existing problems.

6.
Protein & Cell ; (12): 102-119, 2022.
Article in English | WPRIM | ID: wpr-922497

ABSTRACT

The use of two inhibitors of Mek1/2 and Gsk3β (2i) promotes the generation of mouse diploid and haploid embryonic stem cells (ESCs) from the inner cell mass of biparental and uniparental blastocysts, respectively. However, a system enabling long-term maintenance of imprints in ESCs has proven challenging. Here, we report that the use of a two-step a2i (alternative two inhibitors of Src and Gsk3β, TSa2i) derivation/culture protocol results in the establishment of androgenetic haploid ESCs (AG-haESCs) with stable DNA methylation at paternal DMRs (differentially DNA methylated regions) up to passage 60 that can efficiently support generating mice upon oocyte injection. We also show coexistence of H3K9me3 marks and ZFP57 bindings with intact DMR methylations. Furthermore, we demonstrate that TSa2i-treated AG-haESCs are a heterogeneous cell population regarding paternal DMR methylation. Strikingly, AG-haESCs with late passages display increased paternal-DMR methylations and improved developmental potential compared to early-passage cells, in part through the enhanced proliferation of H19-DMR hypermethylated cells. Together, we establish AG-haESCs that can long-term maintain paternal imprints.

7.
Article in Chinese | WPRIM | ID: wpr-912850

ABSTRACT

Objective:Cost accounting for its diagnosis items based on virtual standardized clinical chemistry laboratory.Methods:Relevant data of clinical chemistry laboratories from January to June 2019 were extracted from the laboratory information systems of 10 hospitals in Shanghai, and three health economic experts and the directors of their laboratory departments were interviewed in this regard.On such basis, a virtual standardized clinical chemistry laboratory was constructed. The project cost of the virtual laboratory was calculated from the aspects of supplies exhaust, labor and others. The routine clinical chemistry diagnosis items were clustered according to the principle of laboratory methods, and the cost differences of items in the same cluster were compared using paired t test. Results:The cost of rate method and dry chemical method in testing alanine aminotransferase was 5.12 and 11.63 respectively, and that of immune turbidimetry and immune scattering turbidimetry method in testing immunoglobulin G was 20.00 and 22.26 respectively. Cluster analysis was conducted on 214 routine clinical biochemical diagnostic items, of which 202 items were classified into 42 clusters. The average of clinical chemistry items accounted for 91.7%(4 493/4 900)of the total per day. Based on enzymology, the calculation costs of alanine aminotransferase(rate method), aspartate aminotransferase(rate method), cholesterol(enzyme method)and uric acid(enzyme method)was 5.12, 5.10, 5.24 and 5.14 respectively, presenting no statistical difference( P>0.05). Conclusions:Research on the cost accounting method of clinical chemistry laboratory diagnosis items constructed includes labor cost, reflects the technical labor value of medical staff. Cost accounting based on project clustering can provide references for medical service pricing and financial management of hospitals.

8.
Article in Chinese | WPRIM | ID: wpr-906153

ABSTRACT

Objective:To explore the mechanism of rancidity during storage by researching the changes of water content, relative permeability of cell membrane and rancidity levels of Armeniacae Semen Amarum in deterioration process. Method:Armeniacae Semen Amarum samples under different storage conditions were evaluated and classified by sensory assessors, and samples with different levels of rancidity were obtained. Water content was measured by toluene method, and water activity was obtained by water activity meter. Malondialdehyde (MDA) and relative conductivity were measured using thiobarbituric acid colorimetry and conductivity meter, respectively. The content of fatty oil was obtained by Soxhlet extraction. The acid value and peroxide value were measured in accordance with the general rules 0713 and 2303 of the 2020 edition of <italic>Chinese Pharmacopoeia</italic> (part Ⅳ), respectively. Based on the above experimental data, chemometric methods (cluster analysis, principal component analysis) were selected to establish classification and discriminant models of Armeniacae Semen Amarum with different rancidity levels, in order to verify the accuracy of the classification results. Result:According to the results of sensory evaluation, Armeniacae Semen Amarum samples were divided into three classes, including no rancidity, slight rancidity and rancidity. Compared with the no rancid samples, the color of surface and cotyledon were deepened in rancid samples, and the oil was appeared on surface with rancid taste. The values of water content, water activity, MDA content and relative conductivity were all significantly increased in deterioration process (<italic>P</italic><0.01). The content of fatty oil was significantly decreased with the occurrence of rancidness (<italic>P</italic><0.01), while the acid value and peroxide value increased significantly (<italic>P</italic><0.01). The results of cluster analysis and principal component analysis showed that the rancid samples could be distinguished from the no rancid and slightly rancid samples. Conclusion:The storage conditions under high temperature and high humidity can accelerate the rancidness of Armeniacae Semen Amarum, which is accompanied by the increase of internal water content, the increase of cell membrane permeability and the occurrence of fatty acid rancidity. It is suggested that Armeniacae Semen Amarum should be stored in low temperature, dry environment, as well as short storage time.

9.
Article in English | WPRIM | ID: wpr-897004

ABSTRACT

Purpose@#This study was aimed to evaluate the clinical significance and prognostic value of CRP/albumin ratio (CAR) in patients with gastric cancer. @*Methods@#The data of 205 gastric cancer patients who underwent surgery was analyzed retrospectively. The association of CAR with the clinical features and prognostic value in gastric cancer was analyzed. The data of this study was combined with previous studies to further determine the prognostic value of CAR in patients with gastric cancer using a metaanalysis method. @*Results@#Cox analysis revealed that preoperative CAR was an independent prognosis indicator in patients with gastric cancer. High expression of CAR indicated a shorter survival time than in those with lower expression. CAR has a higher prognostic value in the 1-, 3-, and 5-year overall survival in patients with gastric cancer. CAR showed significant difference regarding the gastric cancer patients’ age, M stage, and clinical stage. The discriminate value of CAR in M stage of gastric cancer was high (area under the curve, 0.809). A meta-analysis combining previous data and our data showed that preoperative CAR demonstrated a significant association with the overall survival of patients with gastric cancer. @*Conclusion@#This study demonstrated that preoperative CAR could serve as an important prognostic indicator in patients with gastric cancer.

10.
Article in English | WPRIM | ID: wpr-889300

ABSTRACT

Purpose@#This study was aimed to evaluate the clinical significance and prognostic value of CRP/albumin ratio (CAR) in patients with gastric cancer. @*Methods@#The data of 205 gastric cancer patients who underwent surgery was analyzed retrospectively. The association of CAR with the clinical features and prognostic value in gastric cancer was analyzed. The data of this study was combined with previous studies to further determine the prognostic value of CAR in patients with gastric cancer using a metaanalysis method. @*Results@#Cox analysis revealed that preoperative CAR was an independent prognosis indicator in patients with gastric cancer. High expression of CAR indicated a shorter survival time than in those with lower expression. CAR has a higher prognostic value in the 1-, 3-, and 5-year overall survival in patients with gastric cancer. CAR showed significant difference regarding the gastric cancer patients’ age, M stage, and clinical stage. The discriminate value of CAR in M stage of gastric cancer was high (area under the curve, 0.809). A meta-analysis combining previous data and our data showed that preoperative CAR demonstrated a significant association with the overall survival of patients with gastric cancer. @*Conclusion@#This study demonstrated that preoperative CAR could serve as an important prognostic indicator in patients with gastric cancer.

11.
Article in English | WPRIM | ID: wpr-880548

ABSTRACT

OBJECTIVE@#To evaluate whether the efficacy of Getong Tongluo Capsule (, GTC, consisted of total flavone of Radix Puerariae) on improving patients' quality of life and lowering blood pressure are superior to the extract of Ginkgo biloba (EGB) for patients with convalescent-phase ischemic stroke and primary hypertension.@*METHODS@#This randomized, positive-drug- and placebo-controlled, double-blind trial was conducted from September 2015 to October 2017. Totally 477 eligible patients from 18 hospitals in China were randomly assigned in a 2:1:1 ratio to the following interventions, twice a day for 12 weeks: (1) GTC 250 mg plus EGB-matching placebo 40 mg (237 cases, GTC group), (2) EGB 40 mg plus GTC-matching placebo 250 mg (120 cases, EGB group) or (3) GTC-matching placebo 250 mg plus EGB-matching placebo 40 mg (120 cases, placebo group). Moreover, all patients were orally administered aspirin enteric-coated tablets 100 mg, once a day for 12 weeks. The primary outcome was the Barthel Index (BI). The secondary outcomes included the control rate of blood pressure and National Institutes of Health Stroke Scale (NIHSS) scores. The incidence and severity of adverse events (AEs) were calculated and assessed.@*RESULTS@#The BI relative independence rates, the clinical recovery rates of NIHSS, and the total effective rates of NIHSS in the GTC and EGB groups were significantly higher than the placebo group at 12 weeks after treatment (P0.05). The control rate of blood pressure in the GTC group was significantly higher than the EGB and placebo groups at 12, 18 and 24 weeks after treatment (P0.05).@*CONCLUSION@#GTC exhibited significant efficacy in improving patients' quality of life as well as neurological function and controlling hypertension. (Registration No. ChiCTR1800016667).

12.
Neuroscience Bulletin ; (6): 1412-1426, 2021.
Article in English | WPRIM | ID: wpr-922631

ABSTRACT

Endogenously eliminating the hematoma is a favorable strategy in addressing intracerebral hemorrhage (ICH). This study sought to determine the role of retinoid X receptor-α (RXR-α) in the context of hematoma absorption after ICH. Our results showed that pharmacologically activating RXR-α with bexarotene significantly accelerated hematoma clearance and alleviated neurological dysfunction after ICH. RXR-α was expressed in microglia/macrophages, neurons, and astrocytes. Mechanistically, bexarotene promoted the nuclear translocation of RXR-α and PPAR-γ, as well as reducing neuroinflammation by modulating microglia/macrophage reprograming from the M1 into the M2 phenotype. Furthermore, all the beneficial effects of RXR-α in ICH were reversed by the PPAR-γ inhibitor GW9662. In conclusion, the pharmacological activation of RXR-α confers robust neuroprotection against ICH by accelerating hematoma clearance and repolarizing microglia/macrophages towards the M2 phenotype through PPAR-γ-related mechanisms. Our data support the notion that RXR-α might be a promising therapeutic target for ICH.


Subject(s)
Anilides/pharmacology , Cerebral Hemorrhage/drug therapy , Hematoma/drug therapy , Humans , Macrophages , Microglia , Neuroprotection , PPAR gamma , Retinoid X Receptor alpha
13.
Article in Chinese | WPRIM | ID: wpr-878731

ABSTRACT

With the upsurge of medical artificial intelligence,the use of computer vision technology to study medical images,which can effectively help doctors to identify and screen diseases,has become a focus of researchers.This paper summarizes the basic situation,specific information,related research,and data sharing and utilizing ways of foreign breast image datasets.This review provides inspirations for the opening of Chinese medical and health data.


Subject(s)
Artificial Intelligence , Breast/diagnostic imaging
14.
China Pharmacy ; (12): 839-844, 2021.
Article in Chinese | WPRIM | ID: wpr-875817

ABSTRACT

OBJECTIVE:To screen the effective compo nent in antioxi dant active fraction of Pueraria lobata . METHODS :The antioxidant active fraction sample (S1-S20) of 20 batches of P. lobata were prepared. HPLC method was adopted. The determination was performed on SepaxBio-C 18 column with mobile phase consisted of methanol-water (gradient elution )at the flow rate of 0.6 mL/min. The column temperature was set at 25 ℃,and detection wavelength was set at 250 nm. HPLC fingerprints of 20 batches of P. lobata were established by the Similarity Evaluation System of TCM Chromatographic Fingerprints (2012 edition),and common peaks were identified. Cluster analysis ,principal component analysis (PCA)and orthogonal partial least squares discriminant analysis (OPLS-DA)were used to screen the effective components in antioxidant active fraction of P. lobata . RESULTS:There were 18 common peaks in HPLC fingerprints of 20 batches of antioxidant active fraction in P. lobata ,and the similarity was more than 0.99. Eight common peaks were identified ,which were 3′-hydroxypuerarin(peak 2),puerarin(peak 3), 3′-methoxypuerarin(peak 4),daidzein(peak 5),genistein(peak 7),formononetin(peak 11),daidzein(peak 13)and genistein (peak 16). The results of cluster analysis and PCA analysis showed that samples S 1,S3,S4,S6,S8,S18 and S 19 were clustered into one category ,and samples S 2,S5,S7,S9-S17 and S 20 were clustered into one category ;peak 2,peak 3,peak 10,peak 11 and peak 13 had great influence on principal component 1;peak 8 and peak 9 had great influence on principal component 2. OPLS-DA analysis showed that peak 4,peak 3,peak 2,peak 16,peak 13 and peak 11 had great influence on the quality of antioxidant active fraction of P. lobata . CONCLUSIONS : HPLC fingerprint for active fraction of P. lobata is established in the study and 8 components are identified ;among them , com puerarin,3′-hydroxypuerarin,daidzein and formononetin maybe the material basis of antioxidant fraction of P. lobata .

15.
Article in Chinese | WPRIM | ID: wpr-863184

ABSTRACT

Ischemic stroke is a common clinical cerebrovascular disease with the characteristics of high incidence rate, high mortality rate, and high disability rate. Recent studies have shown that noncoding RNAs (ncRNAs) are involved in the pathogenesis of ischemic stroke. By competitively binding miRNAs, it regulates neuronal apoptosis, inflammatory response, angiogenesis and blood-brain barrier damage in the process of ischemic brain injury. This article reviews the role of long noncoding RNAs (lncRNAs) as competitive endogenous RNAs in the pathophysiology of ischemic stroke.

16.
Psychiatry Investigation ; : 432-443, 2020.
Article | WPRIM | ID: wpr-832571

ABSTRACT

Objective@#To systematically evaluate evidence for the use of interventions based on appied behavior analysis (ABA) to manage various symptoms of children with autism spectrum disorder (ASD). @*Methods@#Sensitivity analyses were conducted by removing any outlying studies and subgroup analyses were performed to compare the effectiveness of ABA and early start denver model (ESDM), picture exchange communication systems (PECS) and discrete trial training (DTT). @*Results@#14 randomized control trials of 555 participants were included in this meta-analysis. The overall standardized mean difference was d=-0.36 (95% CI -1.31, 0.58; Z=0.75, p=0.45) for autism general symptoms, d=0.11 (95% CI -0.31, 0.54; Z=0.52, p=0.60) for socialization, d=0.30 (95% CI -0.02, 0.61; Z=1.84, p=0.07) for communication and d=-3.52 (95% CI -6.31, -0.72; Z=2.47, p=0.01) for expressive language, d=-0.04 (95% CI -0.44, 0.36; Z=0.20, p=0.84) for receptive language. Those results suggested outcomes of socialization, communication and expressive language may be promising targets for ABA-based interventions involving children with ASD. However, significant effects for the outcomes of autism general symptoms, receptive language, adaptive behavior, daily living skills, IQ, verbal IQ, nenverbal IQ, restricted and repetitive behavior, motor and cognition were not observed. @*Conclusion@#The small number of studies included in the present study limited the ability to make inferences when comparing ABA, ESDM, PECS and DTT interventions for children with ASD.

17.
Article in Chinese | WPRIM | ID: wpr-849634

ABSTRACT

The extrarenal effects of mineralocorticoid receptor (MR) activation are closely related to the regulation of blood pressure. The activation ability of MR varied with age and gender in blood pressure regulation. MR of different types of cells play an important role in the pathogenesis of hypertension by increasing the expressions of osteogenic transcription factors, epithelial sodium channels, fibrotic substances and fibrogenic factors, change of vascular L-type calcium channels, aggravation of inflammation and oxidative stress, etc., leading to arterial elasticity weakened, myocardial fibrosis, and water sodium retention. Cell-specific MR block provides a theoretical basis for the development of targeted drugs for treatment of hypertension in the future. The effect of cell-specific MR on the regulation of blood pressure has been reviewed in present paper.

18.
Article in Chinese | WPRIM | ID: wpr-846494

ABSTRACT

Objective: To establish X-ray diffraction (XRD) fingerprint of Calamina and its processed products, compare the effects of different processing Methods on the main components of medicinal materials and determine the content of ZnO in the processed products. Methods: XRD was used to analyze 10 batches of Calamina and its processed products, and fingerprints of Calamina and its processed products were established respectively. Six different processing methods were compared, and the content of ZnO in all processed products was determined by K value method. Results: Fingerprints of Calamina and its processed products were preliminarily established. There were 23 common peaks in the fingerprints of Calamina, and there were 10 common peaks in the fingerprints of its processed products. After calcination, the ZnCO3 characteristic peak of the raw material was transformed into the characteristic peak of ZnO; The content of ZnO in the calcined product exceeded 56%. Conclusion: XRD fingerprints could be used for the identification and analysis of Calamina and its processed products. The new and reliable method was provided for quality evaluation of Calamina and its processed products.

19.
China Pharmacy ; (12): 1569-1574, 2020.
Article in Chinese | WPRIM | ID: wpr-822621

ABSTRACT

OBJECTIVE:To explore the mo lecular mechanism of Chaihu shugan powder in the adjunctive treatment of major depressive disorder (MDD). METHODS :Network pharmacology was adopted. The microarray dataset GSE 76826 was downloaded from GEO common database ,and the limma package was used to screen the differently expressed genes in the samples of MDD patients. The active ingredients and corresponding targets of Chaihu shugan powder were screened out based on TCMSP platform. Through the online software venny 2.1.0,the differently expressed genes of MDD and the targets of Chaihu shugan powder were screened,and the common target genes of Chaihu shugan powder for MDD were obtained. The target genes were introduced into Cytoscape 3.6.1 software to construct the network diagram of “Chaihu shugan powder active ingredients-MDD target gene ”. Then , BisoGenet plug-in was used to construct the PPI network ,analyze the topological structure and screen the core target gene. KEGG pathway analysis was conducted. RESULTS :There were 139 active components of Chaihu shugan powder for the treatment of MDD,including quercetin ,kaempferol,luteolin,stigmasterol,etc. There were 8 core target genes ,including NR3C2,DPP4, ALOX5,GSTM1,CCND1,IGFBP3 and so on. They play an important role in the treatment of MDD via signaling pathway such as regulating cell receptor ,purine metabolism ,steroid biosynthesis ,aminoacyl biosynthesis ,etc. CONCLUSIONS :Chaihu shugan powder can play a role in the adjunctive treatment of MDD through multiple targets and pathways ,which can provide a theoretical basis for the follow-up study of its mechanism.

20.
Chinese Journal of Biotechnology ; (12): 2083-2091, 2020.
Article in Chinese | WPRIM | ID: wpr-878468

ABSTRACT

Rabbit haemorrhagic disease virus (RHDV) and myxoma virus (MYXV), are two pathogens that have harmful effect on rabbit breeding and population decline of European rabbits in their native range, causing rabbit haemorrhagic disease (rabbit fever) and myxomatosis, respectively. The capsid protein VP60 of the RHDV represents the major antigenic protein. To develop a recombinant bivalent vaccine candidate that can simultaneously prevent these two diseases, we used the nonessential gene TK (thymidine kinase) of MYXV as the insertion site to construct a recombinant shuttle vector p7.5-VP60-GFP expressing the RHDV major capsid protein (VP60) and the selectable marker GFP. Then the shuttle vector p7.5-VP60-GFP was transfected into rabbit kidney cell line RK13 which was previously infected with MYXV. After homologous recombination, the recombinant virus expressing GFP was screened under a fluorescence microscope and named as rMV-VP60-GFP. Finally, the specific gene-knock in and expression verification of the vp60 and gfp genes of the recombinant virus was confirmed by PCR and Western blotting. The results showed that these two genes were readily knocked into the MYXV genome and also successfully expressed, indicating that the recombinant MYXV expressing the vp60 of RHDV was generated. Protection against MYXV challenge showed that the recombinant virus induced detectable antibodies against MYXV which would shed light on development of the effective vaccine.


Subject(s)
Animals , Blotting, Western , Caliciviridae Infections/veterinary , Hemorrhagic Disease Virus, Rabbit/immunology , Rabbits , Vaccines, Synthetic/immunology , Viral Structural Proteins/genetics
SELECTION OF CITATIONS
SEARCH DETAIL