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Article in Chinese | WPRIM | ID: wpr-774325


OBJECTIVE@#To estimate the size of HLA -Ⅰ class typed platelet apheresis donor bank.@*METHODS@#A total of 16062 blood samples from Chinese Han voluntary unrelated marrow donors in Jiangsu were included in this study. Luminex-SSO was used to detect the HLA -Ⅰ class(A,B locus) antigens. The probability of finding at least one HLA matched unrelated donor was calculated based on the HLA -I class phenotype frequency.@*RESULTS@#The population genetic data of HLA -Ⅰ class in Jiangsu were obtained, the optinal bans size in HLA typed apheresis plateler donor registry databane hrad been estimated by evaluating the population genetic data of HLA-1 class same donor.@*CONCLUSION@#The establishment of HLA-1 class typed apheresis platelet donor bank with a total size of 1500 persons is acceptable, which can satisty the patients with phenotype freguency>0.002 to find at least 1 phenotype same donor in 95% probavility.

Bone Marrow , Bone Marrow Transplantation , HLA Antigens , Histocompatibility Testing , Humans , Plateletpheresis , Registries , Tissue Donors
Chinese Journal of Hematology ; (12): 94-97, 2012.
Article in Chinese | WPRIM | ID: wpr-345933


<p><b>OBJECTIVE</b>To analyze the genetic polymorphism, distribution of haplotypes, common and well-documented (CWD) and rare alleles of high-resolution HLA-A, B and DRB1 alleles by analysis from hematopoietic stem cell (HSC) donors in Jiangsu Han Chinese.</p><p><b>METHODS</b>PCR-sequence-based typing and PCR-sequence specific oligonucleotide probes methods were applied for HLA-A, B and DRB1 high-resolution genotyping of 3238 unrelated healthy donors of hematopoietic stem cells in Jiangsu branch of Chinese National Marrow Donor Program registry.</p><p><b>RESULTS</b>46 alleles of HLA-A,85 HLA-B and 51 HLA-DRB1 locus were found. The frequencies of the most common alleles were A * 11:01 (16.52%), B * 13:02 (11.60%) and DRB1 *07:01 (15.78%). That of the most common haplotype was A * 30: 01-B * 13: 02-DRB1 * 07: 01 (8.87%). 40 alleles of HLA-A,77 alleles of HLA-B, and 47 HLA-DRB1 alleles of HLA-DRB1 were CWD, which account for 99. 8% of total number of samples, and a few rare alleles not reported in Chinese population were found.</p><p><b>CONCLUSION</b>The results of high-resolution, CWD and rare alleles showed the characteristics of HLA distribution in Jiangsu Han population, which may be useful for finding HLA matched unrelated donors, as well as for HLA correlation with population genetics and disease association studies.</p>

Alleles , Asian Continental Ancestry Group , Genetics , Genotype , HLA-A Antigens , Genetics , HLA-B Antigens , Genetics , HLA-DRB1 Chains , Genetics , Haplotypes , Hematopoietic Stem Cells , Humans , Tissue Donors
Chinese Journal of Hematology ; (12): 97-100, 2008.
Article in Chinese | WPRIM | ID: wpr-262923


<p><b>OBJECTIVE</b>To explore the potential of human bone marrow stromal cells (MSCs) as the feeding-layer to promote ex vivo expansion of cord blood CD34+ cells and engraftment of the expanded cells in NOD/SCID mice.</p><p><b>METHODS</b>Human MSCs were routinely isolated and cultured. MSCs at passage 3 were used as feeding-layer for the expansion of cord blood CD34+ cell in the presence of thrombopoietin (TPO), flt3/flk2 ligand (FL), stem cell factor (SCF) and granulocyte-colony stimulating factor (G-CSF). The engraftment potential between unexpanded and expanded cord blood cells transplanted into NOD/SCID mice was compared.</p><p><b>RESULTS</b>The total nucleated cells (TNC), CD34 cells and colony forming units (CFUs) in the MSC feeding culture were increased by 111.6-, 19.3- and 58-fold after 1 week expansion and 532.8-, 41.3- and 563.5- fold increased after 2 weeks expansion respectively as compared with that in non MSC feeding culture. In transplant experiment, the percentage of human CD45+ cells (45.3% -59.1%) in bone marrow of recipient mice transplanted with the MSC feeding expanded cells was the highest in all the groups at six weeks after transplantation.</p><p><b>CONCLUSION</b>Human MSCs enhance CB CD34+ cells in vitro expansion and their capacity of short-term engraftment in NOD/SCID mice.</p>

Animals , Antigens, CD34 , Bone Marrow Cells , Cell Separation , Cells, Cultured , Cord Blood Stem Cell Transplantation , Fetal Blood , Cell Biology , Granulocyte Colony-Stimulating Factor , Pharmacology , Humans , Male , Mesenchymal Stem Cells , Mice , Mice, Inbred NOD , Mice, SCID
National Journal of Andrology ; (12): 566-573, 2005.
Article in Chinese | WPRIM | ID: wpr-339480


<p><b>OBJECTIVE</b>To observe the sexual virility of immature immature male mice were divided into a pre-ablactation group (n 10). The first two groups were immunized with the LHRH fusion proportion of pregnant female mice, the morphological and histological examined to conform the emasculating effect of the vaccine. When ted with testosterone (1.0 ml each) , the post-ablactation ones were rameters.</p><p><b>RESULTS</b>The sexual virility of the immature mice immunized in 3 -4 months.</p><p><b>CONCLUSION</b>The LHRH fusion protein vaccine mice after ablactation, and the sexual virility can recover in the pre-ablactation decrease.</p>

Animals , Antibodies , Metabolism , Female , Gonadotropin-Releasing Hormone , Allergy and Immunology , Male , Mice , Mice, Inbred ICR , Pregnancy , Recombinant Fusion Proteins , Allergy and Immunology , Sexual Development , Allergy and Immunology , Sexual Maturation , Allergy and Immunology , Sperm Motility , Testosterone , Vaccines, Synthetic , Allergy and Immunology
National Journal of Andrology ; (12): 248-251, 2003.
Article in Chinese | WPRIM | ID: wpr-238055


<p><b>OBJECTIVES</b>To observe the change of erythropoietin (EPO) in patients of hypogonadism who received androgen replacement treatment and explore the mechanism of androgen-induced increase of red blood cells and haemoglobin.</p><p><b>METHODS</b>Eight patients with Klinefelter's syndrome, divided into two groups, received TU intramuscular injections of 500 mg or 1000 mg dose, respectively. After three months, seven patients received the second injection of crossover dose. Testosterone levels in serum were measured with RIA before and after the injections treatment. RBC count, impacted volume of blood cells and haemoglobin concentration were measured before treatment and 4, 8 weeks after treatment. At the same interval, EPO levels were measured with ELISA method.</p><p><b>RESULTS</b>Development of the secondary sex characters was improved in all patients after the TU injection. Serum testosterone levels raised significantly and reached the peak one week after the injections. Effective level of testosterone lasted for over 6 weeks. RBC count, impacted volume of blood cells and haemoglobin increased at different degrees after TU injections, but these changes were not significant in statistic(P < 0.05). The increased levels remained for 8 weeks. EPO levels were elevated significantly (P < 0.01 or 0.05) after the TU injection(Pbat > 0.05). The second injection could still make the EPO level go up.</p><p><b>CONCLUSIONS</b>Androgen replacement treatment can increase the EPO levels in patients of hypogonadism, which is one of the mechanism of RBC production increase.</p>

Adolescent , Adult , Enzyme-Linked Immunosorbent Assay , Erythropoietin , Blood , Humans , Injections, Intramuscular , Klinefelter Syndrome , Blood , Drug Therapy , Male , Radioimmunoassay , Testosterone , Blood , Therapeutic Uses