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1.
Article in Chinese | WPRIM | ID: wpr-324284

ABSTRACT

<p><b>OBJECTIVE</b>To establish a method for determining the content of primary chemical constituents in the leaves of Cassia angustifolia.</p><p><b>METHOD</b>The HPLC with Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used , acetonitrile-1% acetic acid (10:90-15: 85-18: 82-20: 80-25: 75) in a gradient manner was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 40 degrees C and detection wavelength at 270 nm.</p><p><b>RESULT</b>The results showed that 5 effective components all separated well and showed good linearity.</p><p><b>CONCLUSION</b>The method was proved to be rapid, sensitive, accurate, credible and repeatable. It can be applied to quality control of Folium Sennae.</p>


Subject(s)
Anthraquinones , Chemistry , Apigenin , Chemistry , Chromatography, High Pressure Liquid , Methods , Glucosides , Chemistry , Naphthalenes , Chemistry , Reproducibility of Results , Senna Extract , Senna Plant , Chemistry , Temperature
2.
Article in Chinese | WPRIM | ID: wpr-324283

ABSTRACT

<p><b>OBJECTIVE</b>To establish a method for determining the content of rubrofusarin gentiobioside in Cassia obtusifolia.</p><p><b>METHOD</b>The HPLC with Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used, acetonitrile-THF-1% acetic acid (18: 3:79) was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 40 degrees 2 and detection wavelength at 278 nm.</p><p><b>RESULT</b>A good linearity was obtained from 0.1-0.5 microg with r = 0.999 9 for rubrofusarin gentiobioside. The average recovery was 101.1%, and RSD was 2.23% (n = 5).</p><p><b>CONCLUSION</b>The method was proved to be simple, rapid, sensitive, precise, reliable and repeatable. It can be applied to the quality control of Semen Cassia.</p>


Subject(s)
Cassia , Chemistry , Chromatography, High Pressure Liquid , Methods , Chromones , Chemistry , Drugs, Chinese Herbal , Chemistry , Glucosides , Chemistry , Reproducibility of Results
3.
Article in Chinese | WPRIM | ID: wpr-737028

ABSTRACT

objective To study the expression of CD44v3 and CD44v6 and its relationship with lymph node metastasis in non small cell lung cancer (NSCLC). Methods Specimens (lung tissues) from 52 c ases of NSCLS and 12 normal lung tissue were used to detect the expression of CD 44v3 and CD44v6 by immunohistochemical method (SP method) and flow cytometry, co rrelation was analysed between the expression of CD44v3 or CD44v6 a nd lymph node metastasis of the lung cancer. Results CD44v3 and CD44v6 were not, or weakly expressed in all normal lung tissues from 12 cases. In contrast, the expression levels of CD44v3 and CD44v6 were obviously higher in lung cancer than that in normal tissue(P<0.05). The expression of CD44v 3 and CD44v6 were much higher in patients with lymph node metastasis than those without lymph node metastasis(P<0.05). Conclusion ① CD44v3 and CD44v6 are expressed with different degree in NSCLC. ②There is a close rel ationship between high expression of CD44v6 and lymph node metastasis, and CD44v 6 may be a co-marker for predicting the potentiality of lymph node metastasis i n lung cancer.

4.
Article in Chinese | WPRIM | ID: wpr-735560

ABSTRACT

objective To study the expression of CD44v3 and CD44v6 and its relationship with lymph node metastasis in non small cell lung cancer (NSCLC). Methods Specimens (lung tissues) from 52 c ases of NSCLS and 12 normal lung tissue were used to detect the expression of CD 44v3 and CD44v6 by immunohistochemical method (SP method) and flow cytometry, co rrelation was analysed between the expression of CD44v3 or CD44v6 a nd lymph node metastasis of the lung cancer. Results CD44v3 and CD44v6 were not, or weakly expressed in all normal lung tissues from 12 cases. In contrast, the expression levels of CD44v3 and CD44v6 were obviously higher in lung cancer than that in normal tissue(P<0.05). The expression of CD44v 3 and CD44v6 were much higher in patients with lymph node metastasis than those without lymph node metastasis(P<0.05). Conclusion ① CD44v3 and CD44v6 are expressed with different degree in NSCLC. ②There is a close rel ationship between high expression of CD44v6 and lymph node metastasis, and CD44v 6 may be a co-marker for predicting the potentiality of lymph node metastasis i n lung cancer.

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