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Clin. biomed. res ; 41(4): 283-290, 20210000. tab, graf
Article in English | LILACS | ID: biblio-1349336


Introduction: Oral lesions are present in approximately 30% of the population worldwide. Although the mouth is an anatomical region that can be easily accessed for direct visual examination, most dentists of dental surgeons have reported difficulties in the diagnosis and management of oral diseases. The primary aim of this study was to assess the completeness of referral forms of the patients referred by dentists from the primary care basic health units to an Oral Medicine service. The secondary aim was to analyze if the complexity of the cases justify the referral to a specialist. Methods: Data from 131 referral forms of patients referred from June 2014 to April 2016 were retrieved from the records. The referral's completeness analysis comprised two stages. Stage 1 mainly comprised patient and applicant's information. In the Stage 2, the documents were scored according to amount of information, including the description of the lesion characteristics and the procedures required for the patients' diagnosis and management. The referral was considered justifiable if some procedures not available at primary care were required for diagnosis or treatment. Results: Five (9.8%) referral forms were considered well filled. Diagnosis agreement was 71.4%. Regarding the need of referral, 40,6% of the cases (n = 50) could be settle at the primary care. Conclusion: In conclusion, few referral forms had high-quality information and the many cases could be managed at primary care health services. (AU)

Humans , Male , Female , Adult , Middle Aged , Aged , Referral and Consultation/statistics & numerical data , Oral Medicine , Primary Health Care , Mouth/physiopathology
Braz. dent. j ; 29(3): 309-315, May-June 2018. tab, graf
Article in English | LILACS | ID: biblio-951550


Abstract The aim of this study was to investigate salivary levels of TGFβ1 and proliferation/ maturation of epithelial mucosa cells in diabetic and hypertensive patients. Design: in this cross-sectional study, whole stimulated saliva and oral mucosa exfoliative cytology specimens were collected from 39 patients that were healthy (control, n=10) or presented history of arterial hypertension (HAS, n=9), diabetes mellitus (DM, n=10) or both (DM+HAS, n=10). Salivary flow rate (SFR), TGFβ1 level in saliva, AgNORs and the epithelial maturation were evaluated. Non-parametric Kruskal-Wallis test, followed by Dunn's multiple comparison post-test and the Spearman test correlation analysis were used. SFR showed a significant decreased in DM and DM+HAS (0.47±0.11 and 0.64±0.43 mL/min) when compared to control (1.4±0.38 mL/min). DM+HAS presented the highest value of TGFβ1 concentration (24.72±5.89 pg/mL). It was observed a positive correlation between TGFβ1 and glycaemia (R=0.6371; p<0.001) and a negative correlation between TGFβ1 and saliva (R=-0.6162; p<0.001) and glycaemia and SFR (R=-0.5654; P=0.001). AgNORs number and status of maturation of mucosa cells were similar for all conditions. DM and DM+HAS presented the lowest SFR, which correlated with increased TGFβ1 levels. Despite the higher TGFβ1 secretion it was not observed changes in the morphology or proliferation of epithelial cells when diabetes or hypertension was present.

Resumo O objetivo deste estudo foi investigar os níveis de TGFβ1 na saliva e a proliferação/maturação das células epiteliais da mucosa em paciente diabéticos e hipertensos. Neste estudo transversal, saliva estimulada e amostras de citologia exfoliativa de mucosa oral foram coletadas de um total de 39 pacientes que se apresentavam saudáveis (controle, n=10) ou com história de hipertensão arterial (HAS, n=9), diabetes mellitus (DM, n=10) ou ambos (DM+HAS, n=10). Taxa de fluxo salivar (SFR), níveis de TGFβ1 na saliva, AgNORs e maturação epitelial foram avaliados. Teste não-paramétrico de Kruskal-Wallis, seguido de comparação múltipla de Dunn e correlação de Spearman foram utilizados para as análises. SFR diminuiu significantemente em DM e DM+HAS (0,47±0,11 e 0,64±0,43 mL/min) quando comparado ao controle (1,4±0,38 mL/min). DM+HAS apresentou os maiores valores de concentração de TGFβ1 (24,72±5,89 pg/mL). Foi observada uma correlação positiva entre TGFβ1 e glicemia (R=0,6371; p<0,001) e uma correlação negativa entre TGFβ1 e saliva (R=-0,6162; p<0,001) e glicemia e SFR (R=-0,5654; p=0,001). Número de AgNORs e o padrão da maturação das células epiteliais foram similares entre os todos grupos. DM e DM+HAS apresentaram os menores valores de SFR, os quais foram correlacionados com o aumento nos níveis de TGFβ1. Apesar da maior secreção de TGFβ1, não foram observadas mudanças na morfologia ou proliferação das células epiteliais quando o paciente apresentava diabetes ou hipertensão.

Humans , Male , Female , Adult , Middle Aged , Saliva/metabolism , Diabetes Mellitus/metabolism , Transforming Growth Factor beta1/metabolism , Hypertension/metabolism , Mouth Mucosa/metabolism , Mouth Mucosa/pathology , Salivation , Secretory Rate , Blood Glucose/metabolism , Case-Control Studies , Cross-Sectional Studies , Antigens, Nuclear , Diabetes Mellitus/pathology , Diabetes Mellitus/blood , Hypertension/pathology
Braz. dent. j ; 28(5): 543-547, Sept.-Oct. 2017. tab, graf
Article in English | LILACS | ID: biblio-888684


Abstract The aim of this study is to evaluate the immunohistochemical expression of E-cadherin, N-cadherin and Bmi-1, and their association with clinical parameters and with the degree of histopathological differentiation in oral squamous cell carcinomas. 65 squamous cell carcinoma samples were used for constructing a tissue microarray block, and then immunohistochemistry was performed for different markers. A semi-quantitative analysis of the amount of positive tumor cells was performed by two blind and calibrated observers (Kappa>0.75). The statistical Mann-Whitney and Kruskal-Wallis tests were used to evaluate the data. The correlation between variables was investigated by the Spearman test, and the significance level set at p<0.05. We observed higher expression of Bmi-1 in tumors located in the palate (p<0.0001). In addition, poorly differentiated tumors had a greater amount of Bmi-1 positive cells (p=0.0011). Regarding the other correlations between variables, no significant associations were detected. In conclusion, poorly differentiated squamous cell carcinomas located in the palate have higher immunostaining of Bmi-1, which can characterize activation of the Epithelial-Mesenchymal Transition process in these tumors.

Resumo O objetivo deste estudo foi avaliar a associação entre a expressão imunoistoquímica de E-caderina, N-caderina e Bmi-1, com os parâmetros clínicos e o grau de diferenciação em carcinomas espinocelulares bucais. Sessenta e cinco amostras foram selecionadas para a construção de um bloco de microarranjo tecidual, e a técnica de imunoistoquímica foi realizada para os diferentes marcadores. Uma análise semi-quantitativa das células tumorais positivas foi realizada por dois observadores calibrados e cegos (Kappa>0.75). Os testes estatísticos Mann-Whitney e Kruskal-Wallis foram utilizados para a análise dos dados e a correlação entre as variáveis foi investigada com o teste de Spearman. O nível de significância foi determinado em p <0.05. Observamos maior expressão de Bmi-1 em tumores localizados em palato (p <0.0001). Além disso, tumores pobremente diferenciados apresentaram maior quantidade de células positivas para Bmi-1 (p=0.0011). Não encontramos outras correlações ou associações significativas. Em conclusão, carcinomas espinocelulares pobremente diferenciados e localizados no palato apresentam maior marcação imunoistoquímica de Bmi-1, o que pode caracterizar a ativação do processo de transição epitélio-mesênquima nesses tumores.

Humans , Male , Female , Adult , Middle Aged , Aged , Aged, 80 and over , Carcinoma, Squamous Cell/pathology , Epithelial-Mesenchymal Transition , Mouth Neoplasms/pathology , Biomarkers, Tumor/metabolism , Cadherins/metabolism , Immunohistochemistry , Polycomb Repressive Complex 1/metabolism , Tissue Array Analysis
J. appl. oral sci ; 25(3): 318-323, May-June 2017. tab, graf
Article in English | LILACS, BBO, BNUY, BNUY-Odon | ID: biblio-893624


Abstract Objectives To evaluate the number of AgNORs per nucleus and the expression of Ki-67 at the tumor invasion front (TIF) in relation to clinical parameters (TNM), TIF classification and the prognosis of oral squamous cell carcinomas in an Uruguayan population. Material and Methods This study was conducted through a retrospective survey from 2000 to 2010 at the National Institute of Cancer Montevideo, Uruguay and included 40 patients. The samples were obtained from the resection of the tumor and the TIF was defined according with Bryne, et al.5 (1992). Expression of Ki-67 was assessed by the percentage of positive tumor cells and the AgNOR was recorded as the mean AgNOR (mAgNOR) and the percentage of AgNOR per nucleus (pAgNOR). All analyzes were performed by a blinded and calibrated observer. Results No statistically significant association was observed between immunostaining of Ki-67 and AgNOR with the different types of TIF, regional metastasis and patients prognosis, however it was observed an increase in Ki-67 expression associated with worse patient's clinical staging, although not statistically significant. Conclusions Our results suggest that proliferation markers as AgNOR and Ki-67 are not prognostic markers at the tumor invasive front of carcinoma of oral squamous cell.

Humans , Male , Female , Adult , Middle Aged , Aged , Aged, 80 and over , Mouth Neoplasms/pathology , Carcinoma, Squamous Cell/pathology , Antigens, Nuclear/analysis , Prognosis , Reference Values , Uruguay , Immunohistochemistry , Biomarkers, Tumor , Reproducibility of Results , Retrospective Studies , Analysis of Variance , Ki-67 Antigen/analysis , Tumor Burden , Cell Proliferation , Neoplasm Invasiveness/pathology
Braz. dent. j ; 28(1): 82-84, Jan.-Feb. 2017. graf
Article in English | LILACS | ID: biblio-839106


Abstract Most Departments of Pathology around the world have a considerable archive of formalin-fixed paraffin-embedded (FFPE) tissue suitable for molecular assessment. This article points out the potential DNA damage that may occur if basic steps are not followed during processing and storage of these samples. Furthermore, it hopes to establish parameters to optimize quality and quantity of DNA extracted from FFPE tissues.

Resumo A maioria dos Departamentos de Patologia em todo o mundo têm um considerável acervo de tecidos embebidos em parafina e fixados em formalina, que são passíveis para análises moleculares. Este artigo apresenta os danos ao DNA que podem ocorrer se passos básicos não forem seguidos durante o processamento e armazenamento destas amostras. Além disso, procura estabelecer parâmetros para otimizar a qualidade e quantidade do DNA extraído de tecidos FFPE.

Humans , Tissue Fixation , Paraffin Embedding
Braz. oral res. (Online) ; 31: e19, 2017. tab, graf
Article in English | LILACS | ID: biblio-839527


Abstract To assess the immunocytochemical and immunohistochemical correlation of adhesion (E-cadherin) and cell differentiation (involucrin) molecules in oral leukoplakia and oral squamous cell carcinoma. Cytological samples and biopsies were obtained from male and female patients aged over 30 years with oral leukoplakia (n = 30) and oral squamous cell carcinoma (n = 22). Cell scrapings and the biopsy were performed at the site of the lesion and histological slides were prepared for the immunocytochemical analysis of exfoliated oral mucosal cells and for the immunohistochemical analysis of biopsy tissues using E-cadherin and involucrin. Spearman’s correlation and kappa coefficients were used to assess the correlation and level of agreement between the techniques. Immunostaining for E-cadherin and involucrin by both techniques was similar in the superficial layers of the histological sections compared with cell scrapings. However, there was no statistical correlation and agreement regarding the immunocytochemical and immunohistochemical expression of E-cadherin and involucrin in oral leukoplakia (R = 0.01, p = 0.958) (Kappa = 0.017, p = 0.92) or in oral squamous cell carcinoma (R = 0.26, p = 0.206) (Kappa = 0.36, p = 0.07). The immunoexpression of E-cadherin and involucrin in tissues is consistent with the expression patterns observed in exfoliated oral mucosal cells, despite the lack of a statistically significant correlation. There is an association of the histopathological characteristics of leukoplakia with the expression E-cadherin and of the microscopic aspects of oral squamous cell carcinoma with immunohistochemical expression of involucrin.

Humans , Male , Female , Adult , Middle Aged , Aged , Cadherins/metabolism , Carcinoma, Squamous Cell/metabolism , Leukoplakia, Oral/metabolism , Mouth Neoplasms/metabolism , Protein Precursors/metabolism , Biomarkers, Tumor/metabolism , Biopsy , Immunohistochemistry , Reference Values , Statistics, Nonparametric
Rev. bras. educ. méd ; 40(3): 487-496, jul.-set. 2016. tab
Article in Portuguese | LILACS-Express | LILACS | ID: biblio-829819


RESUMO Pesquisa exploratória documental de abordagem qualitativa cujo objetivo foi compreender como está se constituindo a formação do estudante de Odontologia em relação ao cuidado em saúde, a partir de uma atividade de ensino interdisciplinar ocorrida no segundo ano da formação do cirurgião-dentista. Foi realizado um levantamento retrospectivo do material produzido pelos estudantes do terceiro semestre do curso de Odontologia da Universidade Federal do Rio Grande do Sul, no Seminário de Integração IV, de 2006 a 2013. O corpus de análise foi formado por relatos de acompanhamento de pacientes e narrativas. Foram analisados 95 relatos de casos de pacientes e 67 narrativas pelo método da análise temática de conteúdo de Bardin. A análise do material resultou em 17 unidades de significação/categorias emergentes. A inclusão das narrativas no Seminário de Integração como estratégia metodológica de ensino na saúde trouxe a qualificação dos relatos e aproximou os estudantes do ser humano a ser cuidado, passando por sua queixa principal, história de vida e subjetividades. Currículos inovadores e integrados na área da saúde devem ser acompanhados permanentemente com base na experiência concreta nesta e em outras instituições de educação superior do País.

ABSTRACT This documentary exploratory research project with a qualitative approach aimed to understand how dental students are being trained in terms of healthcare, by means of an interdisciplinary teaching activity held in the second year of dental school. A retrospective survey was performed from the material produced by the students during the third semester of the dentistry course at the Federal University of Rio Grande do Sul, by means of the IV Integration Seminar held in 2006-2013. The analysis corpus consisted of reports featuring patient monitoring and narratives. We analyzed 95 patient case reports and 67 narratives using the thematic analysis method on Bardin content. Analysis of the material resulted in 17 meaning units/emerging categories. The inclusion of narratives in the Integration Seminar as a methodological strategy for health education facilitated the qualification of reports and brought students closer to the human beings under care, by means of exposing the students to the patients’ chief complaint, life history and subjective factors. The specific experiences of this study as well as those of other Brazilian educational institutions suggest that innovative and integrated curricula in the healthcare field must be implemented on a permanent basis.

Braz. j. oral sci ; 15(1): 27-34, Jan.-Mar. 2016. ilus, tab
Article in English | LILACS, BBO | ID: biblio-830998


Aim: To evaluate the association between oral health status, socio-demographic and behavioralfactors with the pattern of maturity of normal epithelial oral mucosa. Methods: Exfoliative cytologyspecimens were collected from 117 men from the border of the tongue and floor of the mouth onopposite sides. Cells were stained with the Papanicolaou method and classified into: anucleated,superficial cells with nuclei, intermediate and parabasal cells. Quantification was made by selectingthe first 100 cells in each glass slide. Sociodemographic and behavioral variables were collected froma structured questionnaire. Oral health was analyzed by clinical examination, recording decayed,missing and filled teeth index (DMFT) and use of prostheses. Multivariable linear regression modelswere applied. Results: No significant differences for all studied variables influenced the patternof maturation of the oral mucosa except for alcohol consumption. There was an increase of cellsurface layers of the epithelium with the chronic use of alcohol. Conclusions: It is appropriate to usePapanicolaou cytopathological technique to analyze the maturation pattern of exposed subjects,with a strong recommendation for those who use alcohol - a risk factor for oral cancer, in which achange in the proportion of cell types is easily detected.

Humans , Male , Adult , Mouth Mucosa , Multivariate Analysis , Oral Health , Papanicolaou Test , Cell Biology , Pathology/methods , Surveys and Questionnaires
Braz. oral res. (Online) ; 30(1): e94, 2016. tab
Article in English | LILACS | ID: biblio-952062


Abstract The present study aimed to assess the rate of agreement between clinical and histopathological diagnoses and to report the frequency of completed forms for specimens that were subjected to histopathological examination and retrospectively examined. Data from 8,168 specimens submitted to histopathological examination were retrieved from the records. A total of 5,368 cases were included. Agreement was defined based on the definition of lesion nature according to its diagnostic category. Sensitivity, specificity, and positive and negative predictive values were calculated for each diagnostic category. The highest rate of agreement was observed for periapical lesions (92.6%), followed by potentially malignant disorders (90.1%) and non-neoplastic proliferative disorders (89.3%). Low rates of histopathological confirmation of the clinical impression were observed for mesenchymal tumors (25.0%) and cysts (44.2%). Sensitivity values were > 0.70 for all lesions, except for cysts (0.51). Specificity was relatively high, ranging from 0.97 to 1.00. The frequency of incomplete biopsy forms ranged from 16.8% (malignant tumors of oral mucosal epithelium) to 51.0% (nonspecific inflammatory reaction). The most frequently completed biopsy forms corresponded to epithelial malignant tumors (83.2%) and glandular inflammation (72.3%). In conclusion, there was an acceptable level of agreement. The low level of completeness of biopsy forms indicates little awareness about the relevance of gathering detailed information during clinical examination.

Humans , Male , Female , Mouth/pathology , Mouth Diseases/pathology , Reference Values , Biopsy/methods , Retrospective Studies , Sensitivity and Specificity , Diagnostic Errors , Mouth Mucosa/pathology
Braz. oral res. (Online) ; 29(1): 1-8, 2015. tab, graf
Article in English | LILACS | ID: lil-777246


The aim of this study was to evaluate the effects of exposure to cell phone electromagnetic radiation on the frequency of micronuclei, broken eggs cells, binucleated cells, and karyorrhexis in epithelial cells of the oral mucosa. The sample was composed of 60 cell phone users, who were non-smokers and non-drinkers, and had no clinically visible oral lesions. Cells were obtained from anatomical sites with the highest incidence of oral cancer: lower lip, border of the tongue, and floor of the mouth. The Feulgen reaction was used for quantification of nuclear anomalies in 1,000 cells/slide. A slightly increase in the number of micronucleated cells in the lower lip and in binucleated cells on the floor of the mouth was observed in individuals who used their phones > 60 minutes/week. The analysis also revealed an increased number of broken eggs in the tongue of individuals owning a cell phone for over eight years. Results suggest that exposure to electromagnetic waves emitted by cell phones can increase nuclear abnormalities in individuals who use a cell phone for more than 60 minutes per week and for over eight years. Based on the present findings, we suggest that exposure to electromagnetic radiation emitted by cell phones may interfere with the development of metanuclear anomalies. Therefore, it is demonstrated that, despite a significant increase in these anomalies, the radiation emitted by cell phones among frequent users is within acceptable physiological limits.

Adult , Female , Humans , Male , Young Adult , Cell Phone , Cell Nucleus/radiation effects , Electromagnetic Radiation , Mouth Mucosa/cytology , Radio Waves/adverse effects , Chromosome Aberrations , Micronucleus Tests , Mouth Mucosa/radiation effects
Clín. int. j. braz. dent ; 10(2): 226-229, abr. -jun. 2014.
Article in Portuguese | LILACS, BBO | ID: lil-724754


Considerando-se o processo de diagnóstico ampliado em saúde, na primeira consulta odontológica, assim como nas consultas de manutenção é de extrema importância que o profissional, após adequada entrevista com o paciente, ao proceder o exame físico, realize o exame das mucosas. Esse ato tem por objetivo inspecionar todas as áreas de tecidos moles intrabucais, em busca de alterações da normalidade. Paralelamente, a inspeção de tecidos duros é realizada. Os achados mais frequentes no exame das mucosas incluem pequenas alterações anatômicas ou lesões benignas. Entretanto, apesar de, na maioria das vezes, o exame de mucosas apontar para a necessidade de acompanhamento de lesões, essa é a principal manobra para a prevenção de câncer de boca. Em que se pese a baixa prevalência do câncer de boca, quando o diagnóstico não é prontamente realizado, a evolução da doença pode inclusive levar o indivíduo à morte. Assim, o presente texto tem por objetivo alertar os profissionais da odontologia e motivá-los a efetivamente realizar o exame das mucosas como rotina. Essa prática, além de ampliar o espectro de atuação profissional, pode salvar vidas

Considering the comprehensive diagnostic process in health, in the first dental appointment, as well as in the maintenance visits, it is of extreme importance that the professional, after thorough interview with the patient, during the physical examination, perform oral mucosal exam. This has the aim of inspecting all intraoral soft tissues searching for normality alterations. Additionally, hard tissue inspection is performed. The most frequent findings in oral mucosal examination include anatomic alterations or benign lesions. However, despite the fact that, most of the time, oral mucosal examination points to the necessity of observation of lesions, this is the main approach for prevention oral cancer. Even though oral cancer has a low prevalence, when the diagnosis is not promptly performed, the evolution of the disease may lead to death. Therefore, the present text has the aim of alerting dental practitioners, as well as motivating them to effectively perform oral mucosal examination as routine. This broadens the spectrum of professional roles as well as may save lives

Mouth Mucosa , Mouth Mucosa/abnormalities , Mouth Mucosa/injuries
J. appl. oral sci ; 20(3): 369-375, May-June 2012. ilus, tab
Article in English | LILACS | ID: lil-643736


OBJECTIVES: The aim of this study was to analyze the immunolabeling of two cell cycle protein regulators, p53 and p21WAF1, in non-dysplastic leukoplakias with different epithelial alterations: acanthosis, hyperkeratosis and acanthosis combined with hyperkeratosis, and compare them with dysplastic leukoplakias. MATERIAL AND METHODS: This was a prospective cohort study involving 36 patients with oral homogeneous leukoplakias. excisional biopsies were performed and the patients remain under clinical follow-up. The leukoplakias were divided into four groups: 6 acanthosis, 9 hyperkeratosis, 10 acanthosis combined with hyperkeratosis, and 11 epithelial dysplasias. Paraffin-embebeded sections were immunostained for p53 and p21WAF1. Five hundred cells from the basal layer and 500 from the parabasal layer were counted to determine the percentage of positive cells. A qualitative analysis was also carried out to determine the presence or absence of immunohistochemical staining in the intermediate and superficial layers. Groups were compared with ANOVA (p<0.05). Pearson's correlation coefficient was used to test for associations between the two markers, p53 and p21WAF1. RESULTS: No leukoplakia recurred and no malignant transformation was observed whitin a follow-up period of 3-6 years. The mean percentage of p53 staining in the basal and parabasal layers was similar in all groups. p21WAF1 staining differed between layers was as follows: in the basal, only 3 to 4% of cells were stained, while in the parabasal, between 16 and 28% of the epithelial cells were stained in the four different studied groups with no statistically significant difference (p>0.05). CONCLUSIONS: Our findings failed to differentiate the non-dysplastic lesions by means of p53 and p21WAF1 immunostaining, notwithstanding similar profiles between non-dysplastic and dysplastic leukoplakias were observed.

Adult , Aged , Female , Humans , Middle Aged , Cell Cycle Proteins/analysis , /analysis , Leukoplakia, Oral/chemistry , Leukoplakia, Oral/pathology , /analysis , Analysis of Variance , Biopsy , Cell Cycle/physiology , /metabolism , Immunohistochemistry , Paraffin Embedding , Prospective Studies , Biomarkers, Tumor/analysis , /metabolism
Braz. oral res ; 26(3): 235-241, May-June 2012. tab
Article in English | LILACS | ID: lil-622939


The purpose of the present paper was to describe the range of lesions histologically diagnosed in an oral pathology laboratory in southern Brazil. A retrospective study of 8,168 specimen analyses recorded between 1995 and 2004 was conducted. The records were retrieved from the Oral Pathology Laboratory, School of Dentistry, Federal University of Rio Grande do Sul, RS, Brazil. A total of 6,831 valid cases (83.63%) were examined. Of these, inflammatory lesions were the most common occurrences (n = 4,320; 63.24%). Benign and malignant tumors accounted for 7.66% (n = 523) and 1.9% (n = 130) of the occurrences, respectively. Significant associations were observed between nonneoplastic proliferative disorders and benign mesenchymal tumors in females, and between squamous cell carcinoma and leukoplakia in males. Most diagnoses were benign in nature and had an inflammatory etiology. The association of some demographic characteristics with the occurrence of lesions suggests that these characteristics should be considered in performing differential diagnoses.

Adult , Female , Humans , Male , Middle Aged , Mouth Diseases/epidemiology , Pathology, Oral/statistics & numerical data , Age Distribution , Brazil/epidemiology , Carcinoma, Squamous Cell/epidemiology , Carcinoma, Squamous Cell/pathology , Logistic Models , Mouth Diseases/pathology , Mouth Neoplasms/epidemiology , Mouth Neoplasms/pathology , Odds Ratio , Retrospective Studies , Sex Distribution
RFO UPF ; 16(3)set.-dez. 2011.
Article in Portuguese | LILACS-Express | LILACS | ID: lil-621151


Objetivo: Definir o perfil do grupo de risco para leucoplasias bucais a partir do banco de dados dos casos diagnosticados no Laboratório de Patologia Bucal da Faculdade de Odontologia da Universidade Federal do Rio Grande do Sul no período 1999 a 2009. Metodologia: O estudo foi baseado nas fichas de biópsia do arquivo do Laboratório de Patologia da Faculdade de Odontologia da Universidade Federal do Rio Grande do Sul. Os dados dos 177 casos foram tabulados no programa SPSS 14.0®; realizou-se a análise descritiva e aplicou-se o teste estatístico qui-quadrado (x²) com nível de significância de 5%, a fim de se verificar possíveis relações estatísticas entre as variáveis. Resultados: Ao correlacionar a variável localização anatômica e diagnóstico histopatológico e localização anatômica e idade, obteve-se significância estatística. A maior prevalência das lesões encontra-se entre a quinta e a sexta décadas de vida. Conclusão: O grupo de risco para leucoplasias está entre a quinta e sexta décadas de vida, localizadas na gengiva inserida, e a característica histológica mais encontrada foi de hiperplasia epitelial com hiperceratose.

RFO UPF ; 16(1)jan.-abr. 2011.
Article in Portuguese | LILACS-Express | LILACS | ID: lil-593685


Introdução: O cisto periodontal lateral é um tipo de cisto odontogênico, de etiologia desconhecida, cuja prevalência na população não é frequente. Essa lesão ocorre geralmente na região de pré-molares inferiores, é assintomática e, em alguns casos, ocasiona expansão da cortical óssea. Radiograficamente, apresenta radio-lucidez unilocular bem definida. Objetivo e relato de caso: Este estudo tem por objetivo relatar o caso de um paciente do gênero masculino, 44 anos de idade, que procurou atendimento com queixa de aumento de volume associado a desconforto na região do segundo pré-molar inferior direito. Após criterioso processo de diagnóstico, procedeu-se à excisão cirúrgica e o exame microscópico revelou se tratar de cisto periodontal lateral, conforme a hipótese de diagnóstico clínico prévia. Considerações finais: Esse relato de caso clínico discute a importância da utilização de um protocolo de diagnóstico diferencial para estabelecer a conduta terapêutica adequada ao paciente.

Rev. ciênc. méd., (Campinas) ; 16(1): 51-56, jan.-fev. 2007. ilus
Article in English | LILACS | ID: lil-489565


O tumor odontogênico escamoso é uma neoplasia benigna rara que pode ser única ou múltipla. Os autores apresentam uma revisão das características clínicas, radiográficas e histopatológicas do tumor odontogênico escamoso e relatam um caso de um paciente com recidiva com acompanhamento de doze anos e discutem os critérios de diagnóstico e abordagens terapêuticas.

Squamous odontogenic tumor is a rare benign neoplasm and may affect multiple sites in the mouth. The authors review the clinical, radiographic and histopathological features of Squamous odontogenic tumor and report a case of a patient with recurrent Squamous odontogenic tumor and 12 years of followup and discuss diagnostic criteria and therapeutic approaches.

Humans , Male , Adult , Recurrence , Odontogenic Tumors/diagnosis , Odontogenic Tumors/diagnostic imaging
Rev. Fac. Odontol. Porto Alegre ; 48(1/3): 77-81, 2007. ilus
Article in Portuguese | LILACS, BBO | ID: lil-533989


O objetivo deste trabalho é fazer uma revisão de literatura a respeito do ensaio dos micronúcleos, explicando o seu significado e sua aplicação em células esfoliadas da mucosa bucal. Micronúcleo (MN) é um núcleo acessório, originado a partir de fragmentos de cromossomo ou de cromossomos inteiros que não são incluídos no núcleo principal durante a mitose. MNs surgem por alterações genéticas espontâneas ou são induzidos por agentes genotóxicos. A análise dos micronúcleos tem sido utilizada como uma ferramenta importante de biomonitoramento de populações. Estudos demonstram que consumidores de fumo e álcool, assim como grupos expostos a determinados agentes em função de sua ocupação ou estilo de vida apresentam um elevado número de MNs nas células bucais esfoliadas.

The aim of this study is to summarise the literature on micronucleus assay, explaining its meaning and its application in exfoliated oral mucosal cells. Micronuclei (MN) is an extra nuclei, originated from chromosome fragments or whole chromosomes that are not included in the main nuclei during mitosis. MNs arise from spontaneous genetic damage or are induced by genotoxic agents. MN analysis has been used as an important tool to biomonitore populations exposed to life-style agents. Studies demonstrate that tobacco and alcohol users, as well as occupationally exposed groups present increased number of MNs in exfoliated oral mucosa cells. Despite the fact that the role of MN frequency has not yet been fully understood, the MNs assay is considered to be an effective biomarker of oral squamous cell carcinoma risk factors effects.

Chromosome Breakage , DNA Damage , Mouth Mucosa , Micronucleus Tests
Braz. oral res ; 21(3): 216-221, 2007. ilus, graf, tab
Article in English | LILACS | ID: lil-458593


The purpose of this study was to compare histologic and morphometric procedures of bone height measurement. Microscopic measurements are the most frequent methods in periodontal studies with animals, but have limited capacity to identify bone levels associated with both healthy tissues and periodontal disease. Ligatures were placed in the maxillary left second molars of 10 male 60-day-old Wistar rats for 30 days. Left and right maxillary sides of 5 rats were processed for histologic analysis (H), sectioned buccolingually, and stained with HE. The maxillae of the other 5 rats were defleshed and used for morphometric analysis (M). Histometric measurements from the cementoenamel junction to the bone crest were performed. Standardized photographs were used for morphometric analysis. The t test was used for dependent or independent samples (alpha = 0.05 percent). Distances from cementoenamel junction to bone crest were 0.95 ± 0.25 and 1.07 ± 0.30 mm for H and M, respectively. Buccal measurements were 0.92 ± 0.16 and 1.08 ± 0.35 mm for H and M. The values obtained using H and M for areas without ligatures were 0.44 ± 0.15 and 0.47 ± 0.11 mm for lingual measurements and 0.23 ± 0.08 and 0.41 ± 0.10 mm for buccal measurements. No significant differences were found between the two methods in the detection of bone height associated with the placement of ligatures in rats.

O objetivo deste estudo foi comparar procedimentos de medida da altura óssea histológica e morfométrica. O método mais freqüente de mensuração da altura óssea em estudos em animais são as medidas microscópicas. No entanto, tem limitada capacidade de identificar os níveis ósseos associados com os tecidos saudáveis e doença periodontal. Foram colocadas ligaduras no segundo molar superior esquerdo de 10 ratos machos Wistar com 60 dias de vida durante um período de 30 dias. Hemimaxilas direitas e esquerdas de 5 ratos foram processadas para a análise histológica (H), cortadas no sentido vestíbulo-lingual e coradas com HE. Hemimaxilas dos outros 5 ratos tiveram toda a matéria orgânica removida para análise morfométrica (M). Medidas histométricas da junção cemento-esmalte (JCE) à crista óssea alveolar foram realizadas. Fotografias padronizadas foram utilizadas na análise morfométrica. O teste t foi utilizado para amostras dependentes e independentes (alfa = 0,05 por cento). As distâncias entre a JCE e a crista óssea na face palatina foram de 0,95 ± 0,25 e 1,07 ± 0,30 mm para H e M, respectivamente. As medidas vestibulares foram 0,92 ± 0,16 e 1,08 ± 0,35 mm para H e M. Os valores obtidos usando H e M para áreas sem ligadura foram 0,44 ± 0,15 e 0,47 ± 0,11 mm por lingual e 0,23 ± 0,08 e 0,41 ± 0,10 mm por vestibular, respectivamente. Não houve diferenças estatisticamente significativas entre os dois métodos na detecção da altura óssea associada à colocação de ligaduras em ratos.

Animals , Male , Rats , Alveolar Bone Loss/pathology , Periodontitis/pathology , Alveolar Bone Loss/etiology , Bone Resorption/pathology , Disease Models, Animal , Germ-Free Life , Ligation , Maxillary Diseases/etiology , Maxillary Diseases/pathology , Periodontitis/chemically induced , Rats, Wistar
RFO UPF ; 12(1): 56-60, 30/04/2007. ilus
Article in Portuguese | LILACS, BBO | ID: biblio-873332


A lesão de células gigantes periférica é uma enfermidade que envolve os tecidos moles da gengiva ou do rebordo alveolar. Tem etiologia desconhecida, embora seja considerada uma lesão reacional que ocorre em resposta à irritação local crônica, como cálculo subgengival e restaurações mal-adaptadas. Clinicamente, a LCGP apresenta-se como elevação nodular, pediculada ou séssil, de coloração avermelhada a roxa e superfície lisa ou lobulada; pode ou não estar ulcerada e possui evolução lenta e crescimento limitado. O tratamento envolve a remoção cirúrgica da lesão, que pode recidivar em torno de 10 por cento dos casos. Este trabalho tem por objetivo relatar um caso de recidiva de LCGP associada a periodontite

Humans , Male , Aged , Granuloma, Giant Cell , Periodontitis , Recurrence
RGO (Porto Alegre) ; 54(1): 77-80, jan.-mar. 2006. ilus
Article in Portuguese | LILACS, BBO | ID: lil-445010


O Fibro-Odontoma Ameloblástico (FOA) é um tumor benigno de origem odontogênica, encontrado com freqüência na região de molar e ramo mandibular. É descrito como uma lesão de crescimento lento e assintomático, comum em crianças e adultos jovens, com média de idade em tomo dos oito anos. Os autores apresentam um caso atípico, de um paciente do sexo masculino, trinta anos de idade, portador de um FOA de crescimento rápido e de grande extensão, envolvendo a região de maxila, assoalho da órbita e zigoma. A lesão estava associada à presença de um terceiro molar incluso e provocava o deslocamento do primeiro molar superior esquerdo. O tratamento realizado foi a enucleação da lesão e remoção dos dentes envolvidos na mesma. Após dois anos de proservação, os exames clínico e radiográfico demonstraram neoformação óssea, indicando que o tratamento realizado foi eficaz para a cura do paciente.

Humans , Male , Adult , Mouth Neoplasms , Odontogenic Tumors , Odontoma/surgery , Odontoma/diagnosis