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This study was aimed to select the best preparation form of medicinal in camellia cake,based on the optimal antithrombotic agents in oil tea camellia defatted cake to establish the foundation of research.The content of effective index component of the oil tea camellia defatted cake preparation was detected by HPLC-PDA.The quality of medicinal preparation from oil tea camellia defatted cake was characterized based on the content,relative quantity,relative ratio of content,yield rate and antithrombotic effect.The results showed that the effect of antithrombotic of three medicinal preparations from oil tea camellia defatted cake was listed in the following descending order of enrichment,ethanol extract,and water extract.The enrichment was the best preparation form of medicinal in oil tea camellia defatted cake preparation with the highest content of effective index components,the lowest extract rate and the lowest dosage.But it had the best effect of antithrombotic.It was concluded that the significant improvement of efficacy was due to the increase of the content of the components in the preparation and the rationality of the proportion.At the same time,analysis of component quality based on basic composition of drug system through the consideration of effective components of type and quantity,composition and other quality factors can further guide the preparation of drugs.
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This study was aimed to select the best preparation form of Aurea Helianthus in order to provide a scientific basis for research and development of anti-myocardial ischemia drugs.The content of effective index components of preparation of Aurea Helianthus was detected using HPLC-PDA.Acute myocardial ischemia rat model introduced by injecting pituitrin was used to investigate the effect of anti-myocardial ischemia.The quality of preparation from Aurea Helianthus was characterized based on pharmacy and efficacy.And the characterized results were given correlation analysis.The results showed that the HPLC method for quantitative determination was verified by methodology.The average recoveries were in the range of 97.04%-102.44%.All three preparations of Aurea Helianthus had the efficacy of anti-myocardial ischemia in varying degrees.The enrichment had the highest effect with lowest extraction rate and the lowest dosage,which was the best form in medicinal-preparation application of Aurea Helianthus.It was concluded that the content of related drug components was higher with unique proportion in enrichment of Aurea Helianthus which was prepared based on drug system proved a high quality medicine.Therefore,the key to the improvement of efficacy was to explore the fittest proportion and improve the content of related pharmaceutical ingredients pertinently according to drug system.
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Phyllanthus emblica L.is related to traditional tibetan medicine,containing diversified pharmacological and physiological functions,such as anti-tumor effects,anti-inflammatory effects,anti-oxidation funtions,slow down in glycemia and blood pressure and the prevention of cardio-cerebral vascular diseases,etc.In this study,the research progress on the anti-tumor efficacy of monomer compounds and the extraction of Phyllanthus emblica L.were reviewed,providing references for the anti-tumor studies of Phyllanthus emblica L.
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<p><b>OBJECTIVE</b>To establish the method for quantitative determination of sibricose A5 and sibricose A6 in Polygalae Radix by HPLC.</p><p><b>METHOD</b>The ultrasonic extracting method was applied in sample pre-treatment. The HPLC procedure was performed on the chromatographic column of Agela Promosil C18 (4.6 mm x 250 mm, 5 microm), the mobile phase was acetonitrile-0.1% phosphoric acid water solution (10:90). The detection wavelength was 330 nm and flow velocity was 1 mL x min(-1). The column temperature was 30 degrees C.</p><p><b>RESULT</b>The method has good linearity in the ranges of 0.0087-0.0694 g x L(-1) (r = 0.9993) for sibricose A5, 0.0090-0.0723 g x L(-1) (r=0.9991) for sibricose A6. The average recoveries of sibricose A5 and sibricose A6 were 101.7%, 97.87%, with the RSD of 1.7%, 1.6%, respectively.</p><p><b>CONCLUSION</b>The method was simple, quick accurate and reliable. It is appropriate for the quantitative determination of sibricose A5 and sibricose A6 in Polygalae Radix.</p>
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Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Chemistry , Polygala , Chemistry , Polysaccharides , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To develop an HPLC method for quantitative determination of three quassinoids in Brucea javanic.</p><p><b>METHOD</b>The determination was carried out on a phenomenex C18 column (4.6 mm x 250 mm, 5 microm) with gradient elution program of methol-water at a flow rate of 1.0 mL x min(-1), and the detection wavelength was 270 nm.</p><p><b>RESULT</b>Linearites of bruceine D, brusatol and bruceine H were good (r = 0.9996, 0.9996, and 0.9998) in ranges of 2.52-12.60, 2.19-10.95, and 2.91-14.55 microg, respectively. The average recoveries of bruceine D, brusatol and bruceine H were 100.01%, 100.95% and 100.43% respectively, and RSD of the above three compounds were 0.31% (n = 6), 1.7% ( n = 6) and 1.7% (n = 6), respectively.</p><p><b>CONCLUSION</b>The determination results of three batch samples showed that the method was simple, accurate and could be used in the quantitative determination of three components in the B. javanica.</p>
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Brucea , Chemistry , Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Reference Standards , Linear Models , Organic Chemicals , Reference Standards , Quality ControlABSTRACT
Nowadays, tea and the substitution (other kinds of tea) take an important status in the drinking, food, health product and the related industry. The most popular Green tea, Maté originated from South America, Large-leaved Kudingcha, Small-leaved Kudingcha, and the Eagle tea used civilian have many similarities on history, chemical constituents and biological activities. In this article, we summarized the similarities and the characteristics of the Green tea and the other four teas in order to provide scientific evidences for better development of the tea and substitutions.
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Humans , Antioxidants , Pharmacology , Camellia sinensis , Chemistry , Plant Leaves , Chemistry , Tea , ChemistryABSTRACT
ObjectiveTo research the metabolism of components in the Radix of Paeonia lactiflora Pall. Methods(①) we established the HPLC fingerprint of water extract of Paeonia lactiflora Pall. and real-time monitored the chemical composition. (②) We established the HPLC fingerprint of rats' serum samples from hepatic portal vein, serum samples from aorta abdominalis and samples of intestinal absorption of Paeonia lactiflora Pall. (③) On this basis, using the established methods, I-IPLC fingerprint spectrum of serum samples,the sample of herb, the sample after intestinal metabolism, rats' serum samples from hepatic portal vein and rats'serum samples from aorta abdominalis were analyzed and compared in order to infer the metabolism of components in the Radix of Paeonia lactiflora Pall. Results24 compositions were detected, seven of which were metabolized by intestinal flora and could not be absorbed into blood; six of them could not be absorbed directedly into intestinal; eight new compounds were absorbed into blood after bowel metabolism while they were not detected in water extract in Paeonia lactiflora Pall. ConclusionWe could infer the metabolic processes of chemicals of Paeonia lactiflora Pall. in oral administration with this method.
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The plants of the genus Ehretia Linn. composed of about 50 species mainly distributed in tropical Asia and Africa.They have been used as folk medicines or traditional tea to treat various ailments in China for a long time. This contribution reviews the chemical constituents isolated from the plants of the genus Ehretia Linn. and related biological activities of these species in the past few decades. The compounds in the genus mainly belong to the classes of phenolic acids, flavonoids, benzoquinones, cyanogenetic glycosides, and fatty acids. The main biological activities include antioxidant, anti-inflammatory, antisnake venom, and anti-allergic activities.
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<p><b>OBJECTIVE</b>To investigate the chemical constituents of Ehretia thyrsiflora.</p><p><b>METHOD</b>Compounds were isolated with silica gel, Sephadex LH-20 and RP-C18 colum chromatography. Their structures were elucidated by means of physico-chemical properties and spectral analysis.</p><p><b>RESULT</b>Six compounds were isolated and identified as beta-sitosterol (1), ethyl caffeate (2), 2-methoxyl benzoic acid octyl ester (3), tetradecenoic acid, 2,3-dihydroxypropyl ester (4), daucoster (5), allantoin (6).</p><p><b>CONCLUSION</b>Compounds 1-5 were obtained from this species for the first time. Compounds 2-5 were obtained from the genus Ehretia for the first time.</p>
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Allantoin , Chemistry , Boraginaceae , Chemistry , Caffeic Acids , Chemistry , Magnetic Resonance Spectroscopy , Plant Leaves , Chemistry , Sitosterols , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect and mechanism of Huanglian Jiedu Tang active fraction (HIJDTAF) on calcium overloading in neurons.</p><p><b>METHOD</b>Cerebral ischemia was imitated by hypoxia/hypoglycemia damage on fetal rat neurons. Double wavelength fluorospectrophotometry was used to assay the content of calcium in neurons in order to evaluate the effect of HLJDTAF on calcium overloading. Neurons were treated with glutamic acid, potassium chloride (KCl), A23187, caffeine(CAF) and methacholine (Mch) to analysis the related mechanism of HLJDTAF on calcium overloading in neurons.</p><p><b>RESULT</b>HLJDTAF 0.3, 0.15 g x k(-1) could remarkably inhibit the calcium overloading in neurons caused by hypoxia/hypoglycemia, glutamic acid, KCl and A23187. HLJDTAF 0.3 g x kg(-1) could inhibit the increasing of calcium caused by CAF and Mch in the presence of and in the absence of extra-calcium.</p><p><b>CONCLUSION</b>HLJDTAF could remarkably inhibit the calcium overloading in neurons after cerebral ischemia injury, it probably plays the function via several pathways.</p>
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Animals , Male , Rats , Caffeine , Pharmacology , Calcimycin , Pharmacology , Calcium , Metabolism , Cells, Cultured , Drugs, Chinese Herbal , Pharmacology , Glutamic Acid , Pharmacology , Methacholine Chloride , Pharmacology , Neurons , Cell Biology , Metabolism , Potassium Chloride , Pharmacology , Rats, Sprague-Dawley , Spectrometry, FluorescenceABSTRACT
We reviewed the metabolic studies on flavonoids of Radix Scutellariae, and found that intestinal flora has played a very important role in the metabolism on Radix Scutellariae. This provided the theoretical basis for studying the bioavailability and metabolic characteristics of Radix Scutellariae.
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<p><b>OBJECTIVE</b>To study on the chemical constituents of Lobelia chinensis.</p><p><b>METHOD</b>The coloumn chromatographic techniques were applied to isolate constituents, and their structures were elucidated by means of spectral data analysis.</p><p><b>RESULT</b>Sixteen compounds were isolated and identified as daucosterol (1), diosmetin (2), apigenin (3), chrysoeriol (4), loteolin (5), hesperidin (6), loteolin-7-O-beta-D-glucoside (7), apigenin-7-O-beta-D-glucoside (8), linarin (9), diosmin(10), 5,7-dimethoxy-8- hydroxycoumarin (11), palmitinic acid (12), lacceroic acid (13), stearic acid (14), beta-sitosterol (15), daucosterol (16).</p><p><b>CONCLUSION</b>All of these compouds were obtained from L. chinensis for the first time.</p>
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Apigenin , Cholestenones , Flavones , Flavonoids , Glycosides , Hesperidin , Lobelia , Chemistry , Plant Extracts , Pharmacology , SitosterolsABSTRACT
To analysis the articles cited by SCI about metabolism of Traditional Chinese Medicine (TCM) and help researchers studying metabolism of TCM.
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Objective: To study the adscription of plasma effective constituents of rat after oral administration of alkaloid fraction of Nelumbo nucifera.Methods: Based on the established HPLC analytical method of plasma effective constituents,analysis and comparison were carried out among HPLC profi les of plasma samples obtained after oral administration of alkaloid fraction of Nelumbo nucifera,blank plasma,alkaloid fraction of Nelumbo nucifera.Results: Sixteen compounds were detected under the method,three of them were architypes of compound contained in alkaloid fraction of Nelumbo nucifera,and the others were metabolites.Through the comparison of the UV spectra,seven metabolites remain essential structure because the max wavelength was same to the architypes’,the other six metabolites’ structure changed greatly.Conclusion: Through the study of the adscription of plasma effective constituents of rat after oral administration of alkaloid fraction of Nelumbo nucifera,the structure of metabolites can be conjectured,which may be helpful to study the ADME and mechanism of action.
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Objective:To establish a method for controlling the quality of the stilbene fraction of Polygonum multiflorum T hunb. Methods: The contents of total stilbene and 2,3,5,4,-tetrahydroxy-stilbene-2-O-?-D-glucoside were determined by UV and HPLC respectively. Results: As a reference, 2,3,5,4,-tetrahydroxy-stilbene-2-O-?-D-glucoside had a good linear relationship in the range of 3.04-15.20?g/ml and 0.1032-0.6192?g, the linear regression equation was Y=0.0588X+0.0026(r=0.9998) and Y=3614089.84X+14475.21(r=0.9997), the average recovery was 99.95% with RSD=2.11%(n=6)and 100.08% with RSD=2.83%(n=6)for the determination of total stilbene and 2,3,5,4,-tetrahydroxy-stilbene-2-O-?-D-glucoside respectively. Conclusion: The determination results of 3 batches of sample showed that the method was simple, convenient and accurate, and could be used for the quality control of the stilbene fraction of Polygonum multiflorum Thunb.
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Orthogonal design was used to study the extraction technology of Qingyan Xiehuo Granule. The solvent volumn, extracting time, extracting times and smashing degree (only for Fructus Canarii) were treated as the factors and examined at various levels. The extraction technology for Folium Polygoni tinctorii and Radix Scutellariae was determined. The methonal extract absorption (at 310nm) of the dried water extract of Folium Polygoni tinctorii and the content of Baicalin were used as the criteria.
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Objective To study the chemical constituents in the leaves of Nelumbo nucifera. MethodsCompounds were repeatedly purified by chromatography and structures were elucidated by physicochemical properties and spectroscopic analyses. Results Nine alkaloids were isolated and identified as 2-hydroxy-1-methoxy-6-methyl-6a,7-dehydroaporphine (1),armepavine (2),dehydroroemerine (3),dehydronuciferine (4),2-hydroxy-1-methoxyaporphine (5),liriodenine (6),pronuciferine (7),roemerine (8),and nuciferine (9). Conclusion compound 1 is a new aporphine alkaloid named nelumnucine.
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Object To study the chemical constituents with the antiviral effect from Yinqiaosan on influenza virus Methods Isolation of the constituents by different kinds of column chromatography and elucidation of their structures on the basis of chemical and spectral methods Results Four flavonoid glycosides were isolated and elucidated as 2′ O D apiofuranosyl (1→2) ? D glucopyranosyl] isoliquiritigenin, 4′ isoliquiritigenin, 4′ O D apiofuranosyl (1→2) ? D glucopyranosyl] isoliquiritigenin, 4 isoliquiritigenin, 4 O D apiofuranosyl (1→2) ? D glucopyranosyl] isoliquiritigenin and 4′ isoliquiritigenin and 4′ O D apiofuranosyl (1→2) ? D glucopyranosyl] liquiritigenin, respectively liquiritigenin, respectively Conclusion 2′ O D apiofuranosyl (1→2) ? D glucopyranosyl] isoliquiritigenin is a new naturally compound isoliquiritigenin is a new naturally compound
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Objective To determine the contents of four fiavonoids(trifolirhizin,nor-kurarinone,xanthonumal and kuraridin)in 25 samples of Sophora flavescens Air.in the market.Methods The determination was carried out on Phe- nomenex Luna C_(18)column(250 mm?4.6 mm,5?m),using Acetonitrile-H_2O as mobile phase,at a flow rate of 1.0 mL/min and detected at the wavelength of 310 nm for trifolirhizin,294 nm for nor-kurarinone and,365 nm for xan- thonumal and kuraridin,and column temperature at 35℃.Results The contents of 4 flavonoids in 25 samples of Sophora flavescens Air.in market were in the ranges of 0.05 %~0.32 % for trifolirhizin,0.05 %~0.51% for nor- kurarinone,0.55?10~(-3)%~4.87?10~(-3)% for xanthonumal and 0.06 %~0.23 % for kuraridin.Conclusion The contents of 4 flavonoids in the samples from the market vary notably,and this should be taken into the quality control of Sophora flavescens Ait.
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AIM: To study the chemical constituents with antiviral activity from Lianqiao Bingduqing Capsule (Fructus Forsythiae) on influenza virus. METHODS: Constituents were isolated by different kinds of column chromatography and their structures were elucidated with chemical and spectral methods. RESULTS: Ten chemical constituents were isolated and elucidated such as forsythoside D, caffeic acid, (+)-phillyrin, (+)-pinoresinal-?-D-glucoside, ?-Hydroxyacteoside, forsythoside C, rutin, forsythoside A. CONCLUSION: ?-Hydroxyacteoside is obtained from Forsythia suspense (Thunb.) Vahl for the first time.