ABSTRACT
Photoautotrophic cultivation with heterotrophic cells as seeds (heterotrophic cells/photoautotrophic cultivation) is an effective way for the development of microalgal biofuel, but its development potential from the point of process optimization has not been investigated in literatures. To evaluate this, the optimizations of medium and culture conditions for Chlorella ellipsoidea were studied. In the heterotrophic stage, the biomass concentration reached 11.04 g/L with the optimized medium in flask, which were 28.0% higher than that with the original medium, and the biomass concentration reached 73.89 g/L in 5-L fermenter. In the photoautotrophic stage, the culture medium and conditions were studied in a 2-L column photobioreactor. The maximum biomass concentration, lipid content and lipid productivity reached 1.62 g/L, 36.34% and 6.1 mg/(L·h) under the optimal photoautotrophic conditions. The lipids were mainly composed of C16-C18 fatty acids, which were raw material suitable for biodiesel. After optimization, heterotrophic cells/photoautotrophic cultivation can significantly improve the capacity of biofuel production by Chlorella ellipsoidea, this method is also expected to be an efficient way for the cultivation of other microalgae that can grow heterotrophically.
Subject(s)
Biofuels , Biomass , Cell Culture Techniques , Chlorella , Metabolism , Culture Media , Fatty Acids , Heterotrophic Processes , Lipids , PhotobioreactorsABSTRACT
Objective To compare the outcomes of traumatic flail chest with multiple injuries treated by operative fixation versus conservative approach. Methods The clinical data of 60 patients with traumatic flail chest with multiple injuries were retrospectively analysed, and the outcomes between operation group (treated by operative fixation, n=32)and non-operation group (treated by conservative approach, n=28) were compared. Results The mean time of hospital stay, ICU stay and mechanical ventilation was significantly shorter, and the prevalences of chest wall deformity, pulmonary infection, pulmonary atelectasis and respiratory failure were significantly lower in operation group than those in non-operation group (P<0.05). Six months after discharge, the pulmonary function parameters such as inspiratory capacity, forced vital capacity, forced expiratory volume in one second, peak expiratory flow, total lung capacity and maximal midexpiratory flow were significantly higher in operation group than those in non-operation group (P<0.05). Conclusion Traumatic flail chest with multiple injuries treated by operative fixation may lead to less flail chest associated complications. Operative fixation has short- and long-term benefits to flail chest.
ABSTRACT
To meet the challenge of the clinical teaching,the"Regulations on the Management of Clinical Teaching Practice in Medical Education"has been made and implemented, which establish the definitude responsibilities,rights and profit relations among doctors,students and the patients. Related organizations and persons should fully realize its normative significance,implementation obstacles and deepen the study of clinical teaching research,thus facilitating the enhancement of the teaching effect,and building a harmonious triangular relationship among the concerned parties.
ABSTRACT
The authors first give a detailed account of the background against which humanistic medicine came into being and then expound the connotations of humanistic medicine. These include the following aspects. The first one is humanism and the humanistic spirit. The second one is humanistic medicine and the three implications it embodies. The authors argue that humanistic medicine, taken as the science of benevolence, ought to regard the patient as the unity of three aspects, physiological, psychological and social. The third one is medical humanity, viz. medical humanity is indispensable to the advancement from physiological medicine to humanistic medicine. As to what disciplines are involved in medical humanity, no final conclusion has yet been reached on this matter so far.
ABSTRACT
The core of the ethical and legal issues in the treatment and recovery of patients with mental disorders is the balance between public security and individual interests. In the treatment and recovery of patients with mental disorders, it is imperative to adhere to the principles of respecting basic hmnan rights and respecting the freedom, dignity, equality and rights of the individual, protect the privacy of the patient, stick to the combination of hospital recovery and community recovery, and strictly define the boundary between willing treatment and unwilling treatment. The aim is to promote the recovery and return of patients to society. At the same time, it is necessary to put issues of mental hygiene in China on the demestic legislative agenda and set up a specialized multi-level legal system of mental hygiene.