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Article in Chinese | WPRIM | ID: wpr-333794

ABSTRACT

<p><b>OBJECTIVE</b>To establish a sensitive and direct method for detecting the activation of protein kinase C (PKC) using fluorescence resonance energy transfer (FRET) technique.</p><p><b>METHODS</b>HEK293 cells were transfected with C kinase activity reporter (CKAR) plasmid or/and parathyroid receptor 1 plasmid , and after incubation for 72 h, the fluorescence resonance energy transfer was measured with or without parathyroid or TPA stimulation.</p><p><b>RESULTS</b>TPA reduced the efficiency of FRET and increased the emission ratio of CFP/YFP (C/Y) in HEK293 cells transfected with CKAR. PTH(1-34) could increase the emission ratio of C/Y in HEK293 cells co-transfected with CKAR and PTHR1 but not in cells transfected with CKAR.</p><p><b>CONCLUSION</b>FRET analysis using CKAR can be utilized to detect the activation of PKC, which provides a useful means for studying the signaling pathways associated with PKC.</p>


Subject(s)
Humans , Bacterial Proteins , Chemistry , Enzyme Activation , Fluorescence Resonance Energy Transfer , Methods , Genes, Reporter , HEK293 Cells , Luminescent Proteins , Chemistry , Protein Kinase C , Metabolism
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