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1.
Acta Pharmaceutica Sinica ; (12): 600-603, 2012.
Article in Chinese | WPRIM | ID: wpr-276274

ABSTRACT

This study is to investigate the protective effect of longistyline A against corticosterone-induced neurotoxicity in PC12 cells. While PC12 cells were exposed to 100 micromol x L(-1) corticosterone for 48 h, cell survival rate was reduced and lactate dehydrogenase (LDH) release increased. In parallel, corticosterone caused significant elevations of DNA fragmentation, [Ca2+]i and caspase-3 activity. However, when the PC12 cells were incubated with longistyline A (4.0, 8.0 and 16.0 micromol x L(-1)) in the presence of 100 micromol x L(-1) corticosterone for 48 h, the effects were evidently alleviated, but dose-dependent manner was not obvious. In summary, longistyline A could generate a neuroprotective effect against corticosterone-induced neurotoxicity in PC12 cells possibly by decreasing [Ca2+]i and caspase-3 activity.


Subject(s)
Animals , Cajanus , Chemistry , Calcium , Metabolism , Caspase 3 , Metabolism , Cell Survival , Corticosterone , Toxicity , DNA Fragmentation , L-Lactate Dehydrogenase , Metabolism , Molecular Structure , Neuroprotective Agents , Pharmacology , PC12 Cells , Phenols , Pharmacology , Plant Leaves , Chemistry , Plants, Medicinal , Chemistry , Rats
2.
Article in Chinese | WPRIM | ID: wpr-264876

ABSTRACT

<p><b>OBJECTIVE</b>To explore genetic relationships of the 39 materials in six species of Curcuma.</p><p><b>METHOD</b>The peroxidase isozyme (POD) and esterase isozyme (EST) were studied using vertical slab polyacrylamide gel electrophoresis (PAGE) technique, and the zymograms were analyzed using the software of NTSYSpc2. 1.</p><p><b>RESULT</b>The interspecific zymogramatic differences were obvious. Each species possessed its own specific zymogram distinguishing form the others. In the analysis of EST isozyme, C. phaeocaulis, C. wenyujin, C. kwangsiensis and C. chuanhuangjiang had their own specific zymogram. In the analysis of POD isozyme, just C. phaeocaulis and C. kwangsiensis had their specific zymogram.</p><p><b>CONCLUSION</b>The genetic relationships are not associated with the geographical distributions and the genetic relationship between C. longa and C. sichuanensis are very close.</p>


Subject(s)
Cluster Analysis , Curcuma , Classification , Genetics , Electrophoresis, Polyacrylamide Gel , Esterases , Genetics , Isoenzymes , Genetics , Peroxidase , Genetics , Phylogeny , Species Specificity
3.
Article in Chinese | WPRIM | ID: wpr-246066

ABSTRACT

<p><b>OBJECTIVE</b>To study the numbers of chromosome in Chinese herb Salvia miltiorrhiza from 7 provinces in China, and S. flava as well as S. evansiana from Yunnan province in China.</p><p><b>METHOD</b>The young root was treated with the mixture of ice and water for 24 h, fixed with Carony's fixative for 6-12 h. After differentiating for 10-12 min with 1 mol x L(-1) hydrochloric acid at 60 'C and staining with carbol fuchsin,the section was observed under microscope.</p><p><b>RESULT</b>Chromosome numbers of S. miltiorrhiza and S.flava were 2n = 2x = 16. The numbers of S. evansiana were 2n = 4x = 32. The basic numbers of the chromosomes were x = 8. And tetraploids were observed in S. miltiorrhiza from Sichuan provices and Shandong provices.</p><p><b>CONCLUSION</b>The basic number of the chromosomes are x = 8. The chromosome numbers of S. miltiorrhiza, S.flava and S. evansiana are 16,16 and 32 respectively. As the chromosomes are the small or micro-small ones, it is difficult to use them for karyotype.</p>


Subject(s)
Chromosomes, Plant , Genetics , Diploidy , Plant Roots , Genetics , Plants, Medicinal , Genetics , Polyploidy , Salvia miltiorrhiza , Classification , Genetics , Species Specificity
4.
Article in Chinese | WPRIM | ID: wpr-282259

ABSTRACT

<p><b>OBJECTIVE</b>To study the yield of different Houttuynia cordata and the changing regularity of their main officinal components in different periods, and provide a basis for harvesting in good time and breeding new varieties.</p><p><b>METHOD</b>The main officinal composition and wet weight of overground parts per acreage of different accessions were determined periodically. The plot yields were tested when harvested and analyzed by analysis of variance.</p><p><b>RESULT</b>The difference of the yields of different accessions was significant. The varying tendency of the content of main officinal component methyl nonyl ketone and its total content per acreage of different accessions were different.</p><p><b>CONCLUSION</b>The integrate characters of Qionglai and Wangyu wild accessions are relatively better, worthy to be widely applied in production.</p>


Subject(s)
China , Drugs, Chinese Herbal , Ecosystem , Plant Components, Aerial , Chemistry , Plants, Medicinal , Chemistry , Saururaceae , Chemistry , Seasons , Species Specificity
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