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1.
Article in Chinese | WPRIM | ID: wpr-872716

ABSTRACT

Shengjiangsan was recorded in a medical book Treatise on Cold-induced Disorders and Plague written in the Qing Dynasty. This prescription, consisting of four drugs: Bombyx, cicada slough, Turmeric and Rhubarb, is simple but effective in regulating Qi and eliminating pathogenic factors. In recent years, the researches on the mechanism of this prescription have been continuously expanded and deepened. According to the collected data, Shengjiangsan is widely used in the treatment of various clinical diseases. The concept of systemic inflammatory response syndrome (SIRS) was first proposed in the 1990s, and it refers to the pathophysiological state caused by self-amplified cascade inflammatory response and inflammatory factor storm arising from infectious or non-infectious factors. In recent years, the treatment of SIRS has gradually become the current focus and hotspot. Patients with severe acute respiratory syndrome (SARS) and novel coronavirus pneumonia (COVID-19) often die from SIRS progression to multiple organ dysfunction syndrome (MODS) or even multiple organ failure (MOF). Interleukin -1 receptor antagonist, anti-bradykinin, platelet activating factor receptor antagonist and other commonly used drugs in clinical treatment of SIRS have not achieved satisfactory results, so it is urgent to find drugs to improve SIRS status. Shengjiangsan is recommended as an initial prescription in traditional Chinese medicine diagnosis and treatment plan for SARS and COVID-19, and its antiviral effect may be related to preventing SIRS from transforming into MODS. Pharmacological studies have shown that Shengjiangsan can regulate the immune imbalance of SIRS to achieve a balance by intervening various inflammatory factors and their signaling pathways. At present, there are few reports on the treatment of SIRS with Shengjiangsan. Therefore, the theoretical analysis of Shengjiangsan and its clinical research and pharmacological mechanism in the treatment of SIRS are discussed here to broaden the thinking for clinical practice and experimental research.

2.
Article in Chinese | WPRIM | ID: wpr-828004

ABSTRACT

To reveal the processing mechanism of Chrysanthemi Flos from the changes of chemical compositions after frying and its effect on the efficacy of liver protection. Ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry(UPLC-Q-TOF-MS) and ultra high performance liquid chromatography(HPLC) were used for the qualitative and quantitative researches of chemical compositions before and after Chrysanthemi Flos frying. Progenesis QI and SPSS software were used for principal component analysis(PCA), partial least squares discriminant analysis(PLS-DA), variable importance projection(VIP) analysis and t-test to identify the compositions with significant changes. Pharmacodynamics experiment was used to investigate the protective effect of crude and fried Chrysanthemi Flos on CCl_4-induced acute liver injury in mice. According to mass spectrometry data, there were 28 chemical compositions in crude and fried Chrysanthemi Flos, mainly including flavonoids and organic acids. 13 compositions such as luteolin, apigenin and luteolin glycoside were increased significantly after frying, while 7 compositions such as chlorogenic acid, luteolin-7-O-glucuronide and apigenin-7-O-glucuronide were decreased significantly after frying. Through principal component analysis, crude and fried Chrysanthemi Flos products were divided into two categories, indicating that there were internal differences in quality. The results of liver injury protection experiment in mice showed that the AST, ALT and MDA contents were significantly decreased and SOD level was increased in mice with liver injury in both the high and medium dose groups. Histopathological examination showed that crude and fried Chrysanthemi Flos can protect the liver by reducing inflammatory cell infiltration, reducing steatosis, and repairing damaged liver cells. The results of this study showed that the chemical compositions had obvious changes after frying, and both crude and fried Chrysanthemis Flos had protective effects on CCl_4-induced acute liver injury in mice. In addition, in the range of high, medium and low doses, the liver protection effect of crude and fried Chrysanthemi Flos increased with the increase of dose. The experiment results provided reference for the mechanism of fried Chrysanthemi Flos and clinical selection of processed products.


Subject(s)
Animals , Chromatography, High Pressure Liquid , Chrysanthemum , Flavonoids , Flowers , Chemistry , Liver , Chemistry , Mice
3.
Article in Chinese | WPRIM | ID: wpr-826634

ABSTRACT

The rule and characteristics of clinical acupoint selection were explored in treatment of puerperal insufficient lactation with acupuncture and moxibustion. The clinical articles on the treatment of puerperal insufficient lactation with acupuncture and moxibustion were retrieved from the databases of CNKI, VIP and Wanfang from the date of establishment to June 1, 2019. The articles were screened in accordance with the inclusion and exclusion criteria. The prescriptions of acupuncture and moxibustion were extracted and normalized. Using Microsoft Excel 2016 software, the use frequency, meridians involved and acupoint distributions were analyzed. Using SPSS Statistics 21.0 software, the cluster and factor analyses were conducted. Totally, 102 articles were included,108 acupoint prescriptions were extracted, 65 acupoitns were designed and the total use frequency was 654 times. The top three acupoints used in treatment of puerperal insufficient lactation were Danzhong (CV 17), Rugen (ST 18) and Shaoze (SI 1). The most frequently involved meridians were the stomach meridian, the conception vessel, the small intestine meridian and the liver meridian. The acupoints were mainly distributed in the chest and four extremities. It was showed in cluster analysis that Rugen (ST 18), Shaoze (SI 1), Zusanli (ST 36) and Danzhong (CV 17) represent 3 clusters and a total of 7 common factors were extracted. The acupoint selection is based on syndrome differentiation in treatment of puerperal insufficient lactation with acupuncture and moxibustion, of which, the local acupoints are predominated and the distal acupoints are combined.

4.
Article in Chinese | WPRIM | ID: wpr-878786

ABSTRACT

Orthogonal experiments were used to optimize the process parameters of curcumin TPP-PEG-PCL nanomicelles; the particle size, electric potential and morphology under the electron microscope were systematically detected for the curcumin TPP-PEG-PCL nanomicelles; and the stability and in vitro release of the curcumin TPP-PEG-PCL nanomicelles were investigated. With DID fluorescent dye as the fluorescent probe, flow cytometry was used to study the uptake of nanomicelles by breast cancer cells, and laser confocal microscopy was used to study the mitochondrial targeting and lysosomal escape functions of nanomicelles. Under the same dosage conditions, the effect of curcumin TPP-PEG-PCL nanomicelles on promoting the apoptosis of breast cancer cells was evaluated. The optimal particle size of curcumin TPP-PEG-PCL nanomicelle was(17.3±0.3) nm, and the Zeta potential was(14.6±2.6) mV in orthogonal test. Under such conditions, the micelle appeared as regular spheres under the transmission electron microscope. Fluorescence test results showed that TPP-PEG-PCL nanomicelles can promote drug uptake by tumor cells, escape from lysosomal phagocytosis, and target the mitochondria. The cell survival rate and Hoechst staining positive test results showed that curcumin TPP-PEG-PCL nanomicelles had a good effect on promoting apoptosis of breast cancer cells. The curcumin TPP-PEG-PCL micelles can significantly reduce the mitochondrial membrane potential of breast cancer cells, increase the release of cytochrome C, significantly increase the expression of pro-apoptotic protein Bcl-2 and reduce the expression of anti-apoptotic Bax protein. These test results were significantly better than those of curcumin PEG-PCL nanomicelles and curcumin, with statistically significant differences. The results revealed that curcumin TPP-PEG-PCL nanomicelles can well target breast cancer cell mitochondria and escape from the lysosomal capture, thereby enhancing the drug's role in promoting tumor cell apoptosis.


Subject(s)
Apoptosis , Breast Neoplasms/drug therapy , Cell Line, Tumor , Curcumin/pharmacology , Humans , Lysosomes , Micelles , Mitochondria , Phosphatidylethanolamines , Polyethylene Glycols
5.
Article in Chinese | WPRIM | ID: wpr-774112

ABSTRACT

OBJECTIVE@#To study the correlation of galectin-3 level in bronchoalveolar lavage fluid (BALF) with Mycoplasma pneumoniae (MP) load and cellular immunity of neutrophils and macrophages in the airway in children with refractory MP pneumonia (RMPP).@*METHODS@#A total of 64 children with RMPP who were hospitalized from January 2013 to January 2017 were enrolled. In addition to the conservative medical treatment, all the 64 children with RMPP were given bronchoalveolar lavage in the acute stage (5-7 days after admission) and 48 out of the 64 children were given bronchoalveolar lavage in the recovery stage (10-14 days after admission). Four milliliters of BALF of the affected lung lobe or segment were collected. ELISA was used to measure the level of galectin-3 in BALF supernatant. RT-PCR was used to measure MP load. Hematoxylin and eosin staining was used to measure the percentage of neutrophils and macrophages. Six children with bronchial foreign bodies were enrolled as the control group.@*RESULTS@#The RMPP group had a significantly higher level of galectin-3 in BALF in both the acute and recovery stages than the control group (P0.05). The RMPP group had a significantly higher MP load in BALF in both the acute and recovery stages than the control group (P<0.01), and the MP load in the acute stage was significantly higher than in the recovery stage (P<0.01). In the children with RMPP, galectin-3 level in BALF in the acute stage was positively correlated with MP load and the percentage of neutrophils (r=0.789 and 0.726 respectively; P<0.01).@*CONCLUSIONS@#Galectin-3 is involved in the process of airway inflammation in children with RMPP, and the level of galectin-3 in BALF is positively correlated with MP load. RMPP is a cellular immune inflammatory lesion with the increase of neutrophils and the reduction in macrophages. Galectin-3 is closely associated with neutrophil chemotaxis and luminal infiltration in children with RMPP. MP load gradually decreases with the recovery from RMPP, but it is not completely eliminated by the immune system in the recovery stage. MP infection can increase the consumption of macrophages in children with RMPP.


Subject(s)
Bronchoalveolar Lavage Fluid , Child , Galectin 3 , Humans , Immunity, Cellular , Mycoplasma pneumoniae , Pneumonia, Mycoplasma
6.
Article in Chinese | WPRIM | ID: wpr-773276

ABSTRACT

Bupleuri Radix has both liver protection and hepatotoxicity. Saponins are the main pharmacodynamic and toxic components of Bupleuri Radix. Based on zebrafish physical model and the model of alcoholic fatty liver( AFL) pathology,the liver toxic and protective effect of saikosaponin a( SSa) were assessed. The results indicated that 1. 77 μmol·L-1 SSa showed protective effect to AFL zebrafish. 5. 30 μmol·L-1 SSa was hepatotoxic to healthy zebrafish,but it showed protective effect to AFL zebrafish. 5. 62 μmol·L-1 SSa was hepatotoxic to healthy and AFL zebrafish. This study is benefit for clinical safety of saikosaponin a.


Subject(s)
Animals , Chemical and Drug Induced Liver Injury , Fatty Liver, Alcoholic , Drug Therapy , Oleanolic Acid , Pharmacology , Toxicity , Saponins , Pharmacology , Toxicity , Zebrafish
7.
Article in Chinese | WPRIM | ID: wpr-775936

ABSTRACT

OBJECTIVE@#To observe the difference in clinical efficacy between chicken-claw needling at Shangbaxie (Extra) and Hegu (LI 4) combined with acupuncture at Houxi (SI 3) and the conventional acupuncture at the 3 points for the treatment of hand dysfunction after stroke.@*METHODS@#Forty-two patients were divided into an observation group and a control group according to the random number table, 21 cases in each one. The chicken-claw needling was used at Shangbaxie (Extra) and Hegu (LI 4) on the affected side combined with acupuncture at Houxi (SI 3) in the observation group. The conventional acupuncture was performed at the same point as the observation group in the control group, once a day, 6 days for a course, 1 day of interval after a course, and the therapeutic effect was observed after 5 courses. The simplifying Fugl-Meyer Motor Function Rating Scale (FMA), the modified Barthel index and the Brunnstrom grading criteria were used to evaluate the hand function of the two groups before and after treatment.@*RESULTS@#The FMA score, Barthel index and Brunnstrom grade were improved after treatment in the two groups (all <0.05), and the FMA score, Barthel index and Brunnstrom grade in the observation group were better than those in the control group (all <0.05).@*CONCLUSION@#Chicken-claw needling at Shangbaxie (Extra) and Hegu (LI 4) combined with acupuncture at Houxi (SI 3) can effectively treat hand dysfunction after stroke, and the curative effect is better than the conventional acupuncture at the 3 points.


Subject(s)
Acupuncture Therapy , Hand , Humans , Needles , Stroke , Treatment Outcome
8.
Article in Chinese | WPRIM | ID: wpr-801711

ABSTRACT

Objective: To discuss the clinical effect of Shugan Jieyu capsules combined with repetitivetranscranial magnetic stimulation (rTMS) on depression during perimenopause and neuroendocrine function. Method: One hundred and thirty-two patients were divided into control groupand observation groupby random number table. Patients (65 cases) in observation group got sertraline hydrochloride tablets, 50 mg/time, 2 times/days. Patients (67 cases) in control group got Shugan Jieyu capsules after breakfast and dinner, 2 grains/time, and treatment of rTMS, 20 minutes/time, 1 time/day. And one course of treatment was 5 days, there were a 2-day interval between two courses. And there were a total of 4 courses in the two groups. Both groups were continuously treated for8 weeks. Degree of depression was evaluated by Hamilton depression scale-17 (HAMD-17). Before and after treatment, scores of Hamilton anxiety scale (HAMA), quality index of sleep in Pittsburgh (PSQI), Kupperman and syndrome of kidney deficiency and stagnation of liver were graded. Levels of follicle stimulating hormone (FSH), Luteinizing hormone (LH), estradiol (E2), 5-serotonin (5-HT), dopamine (DA), norepinephrine (NE) and 5-hydroxyindolyl acetic acid (5-HIAA) were detected, and the safety was evaluated. Result: According to the variance analysis of repeated measurements, after treatment, score of HAMD-17 decreased (Pth day after treatment, score of HAMD-17of observation groupwas lower than that in control group (PPχ2=6.405, Pχ2=5.304, PPConclusion: Shugan Jieyu capsules combined with repetitivetranscranial magnetic stimulation can ameliorate symptoms of depression and anxiety, havea bettereffect than Sertraline hydrochloride tablets, and can regulate levels of sex hormone and monoamine neurotransmitter.

9.
Article in Chinese | WPRIM | ID: wpr-753129

ABSTRACT

Objective : To explore clinical value of brain protein hydrolysate (BPH ) and simvastatin in treatment of patients with cerebral infarction (CI) complicated carotid atherosclerotic plaques (CAP).Methods : A total of 90 CI + CAP patients treated in our hospital from Mar 2016 to May 2017 were selected ,randomly and equally divided into routine treatment group and combined treatment group (received simvastatin + BPH based on routine treat-ment) ,both groups were treated for four weeks- Therapeutic effect ,United States National Institutes of Health Stroke Score (NIHSS) ,CAP-related indexes ,hemorheology indexes ,LVEF and CRP before and after treatment and incidence of adverse events were compared between two groups .Results : Compared with routine treatment group after treatment ,there were significant reductions in NIHSS score [ (15-56 ± 6-33) scores vs.(12-14 ± 3-18) scores] ,CAP size [ (1-54 ± 0-32) cm2 vs.(1-34 ± 0-30) cm2 ] ,intima-media thickness [ (1-37 ± 0-08) mm vs. (1-15 ± 0-04) mm] ( P<0-01 all) ,and significant rise in total effective rate (80-00% vs.95-56%, P=0-024) in combined treatment group ; there were significant reductions in hematocrit [ (44-24 ± 3-24 )% vs.(37-72 ± 2-41)%] ,platelet aggregation rate [ (45-62 ± 15-95)% vs.(38-10 ± 2-52)%] ,fibrinogen [ (4-06 ± 1-43) g/L vs. (3-18 ± 1-15) g/L] ,whole blood viscosity [ (2-66 ± 0-77) mPa·s vs.(1-55 ± 0-25) mPa·s] ,plasma viscosity [ (1-45 ± 0-11) mPa·s vs.(1-21 ± 0-16) mPa·s] and serum CRP level [ (4-36 ± 1-03) mg/L vs.(1-70 ± 0-20) mg/L] ,and significant rise in LVEF [ (49-30 ± 3-74)% vs.(56-67 ± 4-37)%] in combined treatment group , P<0-01 all- Incidence rate of cardio-and cerebrovascular events in combined treatment group was significantly lower than that of routine treatment group (4-44% vs.17-18%, P= 0-004 ).Conclusion : Simvastatin combined BPH based on routine treatment can achieve better total therapeutic effect and reduce incidence rate of complications in CI + CAP patients .

10.
Chinese Acupuncture & Moxibustion ; (12): 1285-1288, 2019.
Article in Chinese | WPRIM | ID: wpr-781793

ABSTRACT

OBJECTIVE@#To observe the clinical therapeutic effect of acupuncture at Qianlianquan (Extra) and eight confluent points on chronic pharyngitis.@*METHODS@#A total of 42 patients with chronic pharyngitis were randomized into an observation group and a control group, 21 cases in each one. In the observation group, acupuncture was applied at Qianlianquan (Extra) and eight confluent points [Neiguan (PC 6), Waiguan (TE 5), Lieque (LU 7), Houxi (SI 3), Gongsun (SP 4), Shenmai (BL 62), Zhaohai (KI 6) and Zulinqi (GB 41)], once a day, 6 days as one course, with an interval of 1 day between the courses, 4-course treatment was required. In the control group, granules were taken orally 5 g each time, 3 times a day, 7 days as one course and 4 courses were required. Before and after treatment, symptoms and signs scores of chronic pharyngitis and the contents of IL-2 and TNF-a in serum were compared in the two groups.@*RESULTS@#Compared before treatment, symptoms and signs scores of chronic pharyngitis and the contents of IL-2 and TNF-a in serum were improved in both groups (all <0.05), and the changes in the observation group were superior to the control group (all <0.05). The total effective rate was 90.5% (19/21) in the observation group, which was superior to 81.0% (17/21) in the control group (<0.05).@*CONCLUSION@#The therapeutic effect of acupuncture at Qianlianquan (Extra) and eight confluent points on chronic pharyngitis is superior to conventional Chinese patent medication treatment by granules.


Subject(s)
Acupuncture Therapy , Humans , Pharyngitis , Therapeutics , Treatment Outcome
11.
Article in Chinese | WPRIM | ID: wpr-778304

ABSTRACT

Objective To explore the mediating role of campus bullying in parental psychological control and middle school students’non-suicidal self-injury. Methods Totally 1 497 students in grade 1, 2 and 3 from four middle schools in Shenyang City were selected by using stratified random cluster sampling method and surveyed with questionnaires. Results The incidence of non-suicidal self-injury was 9.9% in middle school students. Being bullied had some mediating effect between parental psychological and non-suicidal self-injury, the mediating effect accounted for 27.4% of the total effects. Bullying had some mediating effect between parental psychological and non-suicidal self-injury, the mediating effect accounted for 26.4% of the total effects. Conclusion Campus bullying (bullied and bullying) plays a multiple mediating role between parental psychological control and non-suicidal self-injury.

12.
Tianjin Medical Journal ; (12): 509-514, 2018.
Article in Chinese | WPRIM | ID: wpr-698054

ABSTRACT

Objective To study the effects of ibuprofen on the growth and development of oligodendrocytes. Methods A total of 6 clean and healthy adult female SD (Sprague Dawley) rats were used for extracting and culturing of oligodendrocytes(OLs).Lysophosphatidic acid(LPA)was then added,and the morphological changes of OLs pre-treatment and post-treatment were observed. Then 6 newborn rats (born 24-48 h) were used for mixed glial cell extraction from the cortex, then the OPCs were inoculated into the culture plates and randomly divided into control group, ibuprofen group, lysophosphatidic acid(LPA)group and LPA+ibuprofen group.After the adhering of the cells in each group for three days, cell morphology was observed,and the drugs were added as interventions.The control group was treated with normal saline, and the other 3 groups were added with saline solution of ibuprofen(100 μmol/L),LPA(1.0 μmol/L)and the mixture of them. The cell morphological changes were observed after 7-day intervention.The morphology of OPCs and OLs were observed by immunofluorescence staining through OPCs'specific immune markers (platelet-derived growth factor receptor alpha, PDGFR-α)and OLs'specific immune markers(myelin basic protein,MBP)along with cell count of mature OLs.Western blot assay was used to detect the relative expression level of MBP in each group. Results After the treatment with LPA to the mature OLs,protrusions were shrinking and became very sparse.The morphology of cells developed well in each group after cell adhering for 3 days. After drug intervention for 7 days, more cell protrusions and branches were observed in ibuprofen group and LPA+ibuprofen group than those of the control group and LPA group.The results of cell count showed that the number of MBP positive cells was significantly higher in the ibuprofen group and LPA+ibuprofen group than that in the control group and LPA group(P<0.01).The results of Western blot assay showed that the MBP protein expression was significantly less in LPA group than the other three groups (P<0.01), and the expression was significantly higher in the ibuprofen group than that of LPA+ibuprofen group (P<0.01). Conclusion LPA has a toxic effect on the growth and development of OPCs, and it has an inhibitory effect on the normal growth of mature OLs. A certain concentration of ibuprofen can significantly inhibit the cytotoxicity of LPA on OPCs and OLs,and promote the formation and maintenance of mature OLs.

13.
Article in Chinese | WPRIM | ID: wpr-690936

ABSTRACT

Current dogma suggests that hematopoietic stem cells (HSC) reside in the top of the hematopoietic hierarchy, which can provide all kinds of mature blood cells constantly through self-renewal and multilineage differentiation potential. HSC has been regarded as the main cell population that maintains the stable hematopoiesis and several differentiation and development patterns of HSC have been summarized based on transplantation results. However, in deed the transplantation experiment is based on an extremely situation of stress which could not really reflect the function of HSC in normal homeostatic condition. Recent studies show that hematopoietic progenitor cells (HPC) play the most important role in hematopoiesis based on different experimental strategies. This article focuses on the controversial subject of the function of HSC and HPC under homostasis.


Subject(s)
Cell Differentiation , Hematopoiesis , Hematopoietic Stem Cells
14.
Article in Chinese | WPRIM | ID: wpr-690707

ABSTRACT

A total of 58 varieties in Lonicera japonica from 20 producing areas were amplified by 22 pairs of SSR primers. Seven pairs of polymorphic primers were screened and their primers were used to establish DNA identity card and analyze genetic similarity.All the 58 varieties could be distinguished each other by the DNA identity card constituted by 7 pairs of core SSR primers.The genetic similarity coefficients of 58 varieties ranged from 0.366 7 to 0.916 7 by using PopGene32(vesion1.32). Furthermore, all the varieties consistency were classified into 4 groups and constructed an evaluation table according to cluster analysis by an un-weighted pair-group average method with arithmetic mean. As expected, the results of cluster and evaluation table reflected 58 varieties relatives, which provide reference information for the selection of fine germplasm of L. japonica and the theoretical basis for the study of Dao-di herbs.

15.
Article in Chinese | WPRIM | ID: wpr-687258

ABSTRACT

Three different beta-glycosidase sequences of Ttebgl3, Tpebgl1 and Tpengl3 from Thermotoga thermarum DSM 5069 and Thermotoga petrophila RKU-1 were analyzed. Also, the influence of temperature, pH, concentration of DMSO, metal ions and kinetic constant on catalytic conversion of baicalin had been compared. The results indicated that the optimal pH and optimum temperature for transformation of baicalin was 4.5 85 °C, 5.0 80 °C and 5.5 80 °C, respectively. The family GH3 beta-glycosidase Ttebgl3 and Tpebgl3 had the better DMSO tolerance. The activation effect of the metal ions on the catalytic conversion of baicalin was not obvious, and the inhibition of the GH3 family beta glucosidase was significantly stronger than that of the GH1 family. The kinetic constants of three different beta-glucosidases catalyzed baicalin were significantly different. The Km and Vmax values of Tpebgl1, Tpebgl3 and Ttebgl3 were 0.029 2 mmol·L⁻¹ 4.85 U·mg⁻¹, 0.268 6 mmol·L⁻¹ 121.04 U·mg⁻¹ and 0.391 8 mmol·L⁻¹ 308.90 U·mg⁻¹, respectively. Family GH3 beta-glycosidase converted more baicalin than family GH1 with the optimal conditions, 0.02 g baicalin, and the conversion rate was 68%, 97.3%, 97.31% respectively. The results of the study provided a guarantee for the transformation of baicalin.

16.
Article in Chinese | WPRIM | ID: wpr-335759

ABSTRACT

To study sesquiterpenes with anti-metastasis breast cancer activity from Chloranthus henryi, ten sesquiterpenes ,zedoarofuran (1), chlorajapolide D (2), 4β, 8β-dihydroxy-5α(H)-eudesm-7(11)-en-8, 12-olide (3), curcolonol (4), lasianthuslactone A (5), chlomultin C (6), (1E,4Z)-8-hydroxy-6-oxogermacra-1(10), 4, 7(11) -trieno-12, 8-lactone (7), shizukanolide E (8) , shizukanolide F (9) , 9α-hydroxycurcolonol (10), and five bis-sesquiterpenes, shizukaol B (11), shizukaol C (12) , cycloshizukaol A (13) , sarcandrolide B (14) , henriol A(15), were isolated by using different kinds of column chromatography methods from the ethyl acetate part of Ch.henryi and their structures were identified based on spectroscopic methods. Compounds 2, 8, 9, and 10 were obtained from the genus Chloranthus for the first time. Compounds 2, 5, 8-10, 12,and 14 were obtained from this plant for the first time. Some isolated compounds were subjected to evaluate the anti-metastasis breast cancer activity by using pharmacological methods, and only compounds 4, 11, and 12 were potent active.

17.
Chinese Pharmacological Bulletin ; (12): 1589-1595, 2017.
Article in Chinese | WPRIM | ID: wpr-667309

ABSTRACT

Aim To explore the pharmacological mechanism of arsenic trioxide (ATO) on endoplasmic reticulum(ER) stress and provide indirect pharmacological indicators for the therapeutic drugs monitoring of acute promyelocytic leukemia.Methods The ATO toxicology database was utilized to associate gene data and establish protein-protein interaction networks.MCODE algorithm was used to analyze clustering of network topology relationship in order to find the critical functional gene group.The HL-60 cells were used as the model in this study.The cytotoxicity of ATO to HL-60 was evaluated by MTT assay.Real-time PCR assay was involved in detecting the expression of stress-related genes in ER stress.Western blot was used to detect the expression of stress-related proteins in ER stress.ER-specific staining was conducted by ER-Tracker Red.Cell differentiation and apoptosis was tested by flow cytometry.Results 206 ATO-related genes had 3 794 pairs of relationships,and ER stress-related module was outstanding among the top 4 gene cluster.ATO inhibited HL-60 cell proliferation in a dose-dependent manner;4 h after administration ATO could induce ER stress.The expressions of GRP78 and GRP94 were significant and the gene expression level changed greatly over time.The expression level of GRP78,GRP94 and Nrf2 was induced by 1 ~4 μmol · L-1 ATO,and the expression of CHOP was significant by 8 μmol · L-1 ATO.ATO could induce the phosphorylation level of eIF2α protein at 1 ~2 μmol · L-1 as well as a large expression of CHOP protein markedly at 8 μmol · L-1.As shown in ER-specific staining,ATO at 4,8 μmol · L-1 induced ER swelling.Flow cytometry showed that ATO could induce cell differentiation at 1 μmol · L-1,and the differentiation group was obvious at 2 μmol · L-1.So as to the concentration of 4 and 8 μmol · L-1,but the apoptotic group emerged at 8 μmol · L-1.Conclusion ATO can inhibit the proliferation of HL-60 and in this process,ER stress is involved,which shows a concentration-dependent phaseswitching effect.

18.
Article in Chinese | WPRIM | ID: wpr-665049

ABSTRACT

Objective:To explore value of digital subtraction angiography(DSA)in diagnosing ischemic cerebrovascu-lar disease(ICD)and evaluating therapeutic effect after intervention.Methods:Clinical data of 134 ICD patients treated in our hospital from Jan 2014 to Jun 2015 were retrospectively analyzed.All patients received magnetic reso-nance angiography(MRA),CT angiography(CTA)and DSA before intervention,and diagnostic value of three methods for ischemic cerebrovascular lesion were compared;stenotic degree of vessels and score of United States National Institutes of Health Stroke Score(NIHSS)were compared between before and after intervention.Results:A total of 1340 vessels were checked in 134 patients.DSA discovered 341 lesioned vessels(25.45%),25 completely occluded vessels(1.87%),153 vessels of 70% ~99% stenosis(11.42%),61 vessels of 50% ~69% stenosis(4.55%) and 102 vessels of <50% stenosis(7.61%);compared with CTA and MRA,there were significant rise in detection rates of lesioned vessels(21.19%,19.40% vs.25.45%),50% ~69% stenosis vessels(3.06%,2.54% vs.4.55%) and <50% stenosis vessels(5.67%,4.93% vs.7.61%)in DSA,P<0.05 or <0.01. A total of 109 patients with 120 vessels received intervention,compared with before operation,there were significant reductions in stenotic rates of extracranial artery[(83.51 ± 8.74)% vs.(22.87 ± 7.90)%]and intracranial artery[(81.63 ± 9.02)% vs. (19.64 ± 6.18)%]after intervention,P=0.001 both.Compared with before intervention,there was significant re-duction in mean NIHSS score[(14.68 ± 3.18)scores vs.(3.16 ± 1.94)scores]on three months after intervention, P=0.001.Conclusion:Detection rate of vascular lesion by DSA is significantly higher than those of CTA and MRA in ICD patients.Postoperative therapeutic effect assessment using DSA possesses high value,which is worth exten-ding.

19.
Chinese Mental Health Journal ; (12): 964-970, 2017.
Article in Chinese | WPRIM | ID: wpr-703957

ABSTRACT

Objective:To investigate the dyadic coping and intimacy in gynecological cancer patients and their partners,and to explore the association between the two aspects.Methods:A total of 180 postoperative couples with gynecological cancer from 4 tertiary hospitals were investigated with Dyadic Coping Inventory (DCI) and Short Marital Adjustment (MAT).The relationship between couples,dyadic coping and intimacy were analyzed with the Actor-Partner Interdependence Model (APIM).Results:APIM result showed that in the actor effect patients' and spouses'supportive dyadic coping,delegated dyadic coping and common dyadic coping were positive associated with their own intimacy (B =1.77-4.41),patients'and spouses'negative dyadic coping were both adversely associated with their own intimacy (B =-2.81,-2.66).The partner effect showed that patients' supportive dyadic coping and delegated dyadic coping were both positive associated with spouses'intimacy (B =1.00,4.07),spouses'delegated dyadic coping and common dyadic coping were both positive associated with patients' intimacy (B =2.67,2.60),patients' and spouses'negative dyadic coping were negatively associated with partner'intimacy (B =-1.67,-1.40).Conclusion:It suggests that the dyadic coping may be associated with both patients'and partners' intimacy.

20.
Article in Chinese | WPRIM | ID: wpr-852991

ABSTRACT

The package leaflet is a legal document relevant with drug information, which plays an important role for instructing the rational use of the medicinal product for the patient population. During the registration application in the EU countries, the patient readability test on package leaflet is the essential requirement to ensure the leaflet information easy-to-understand and avoid any risk caused by misunderstanding. The readability research on package leaflet can improve its quality and drug use safety to patients. Currently, CFDA does not publish any regulation/guidance on the drug application in China. By the successful case of registration application of Danshen Capsule as the herbal medicine in the EU, this paper not only discusses the relevant requirements of readability test in the EU, but also offers the regulatory advice on future regulations of readability research of the pharmaceutical products in China.

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