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1.
Article in Chinese | WPRIM | ID: wpr-735152

ABSTRACT

Objective:To observe the effect of tuina exercise on simple obesity in college students.Methods:Fifty-seven college students with simple obesity were divided into two groups according to the stratified randomization method.Twenty-eight in the tuina exercise group were trained in tuina exercise;while 29 in the auricular acupoint sticking group were treated with acuricular acupoint sticking.The tuina exercise group was trained once every other day,and 10 times made one course.The auricular acupoint sticking was replaced once every 4 d,and 5 times made one course.After 2-course treatment,the total therapeutic effect,weight,body mass index (BMI),waist and hip circumferences,serum total cholesterol (TC),triglyceride (TG),high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were assessed.Results:The total therapeutic effect was 86.2% in the auricular acupoint sticking group and 85.7% in the tuina exercise group.There was no significant difference between the two groups (P>0.05).After treatment,the weight,BMI,waist and hip circumferences were decreased and the differences were statistically significant (all P<0.05).The waist and hip circumferences in the tuina exercise group were lower than those in the auricular acupoint sticking group,showing statistically significant differences (all P<0.05).After treatment,there were no significant intra-group differences in TC,TG,HDL-C and LDL-C in the two groups (all P>0.05),and the between-group differences were not significant (all P>0.05).Conclusion:Tuina exercise has reliable effect in treating obesity.It can produce more significant improvements in waist and hip circumferences than auricular acupoint sticking.But no obvious effect is shown in blood lipid indicators.

2.
Chinese Journal of Stomatology ; (12): 683-688, 2013.
Article in Chinese | WPRIM | ID: wpr-274158

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the tribology characteristics of two ceramic materials in vitro:feldspathic glass-ceramic (veneer porcelain) and lithium disilicate glass-ceramic (heat-pressed ceramic), and to evaluate the wear resistance of different ceramic materials from the dynamic chewing perspective.</p><p><b>METHODS</b>Wear tests were performed in simulated oral environment with stainless steel ball antagonists (r = 3 mm), veneer porcelain (CERAMCO 3) and heat-pressed ceramic (IPS e.max Press HT type) in the chewing simulator. The tribological tests were carried out under artificial saliva lubrication condition in room temperature with a vertical load of 10 N for 1.2×10(6) cycles (f = 1.5 Hz, uniform circular motion, revolving speed = 90 r/min, radius = 0.5 mm). The wear volumes were measured using three-dimensional profiling, and surface microscopic morphology were observed using scanning electron microscopy at time point of 200 000, 400 000, 600 000, 800 000, 1 000 000, and 1 200 000 cycles.</p><p><b>RESULTS</b>In a simulated oral environment, the wear rates of veneer porcelain were (0.001 20 ± 0.00 018) , (0.000 10 ± 0.000 03) , (0.000 50 ± 0.000 05), (0.000 10 ± 0.000 02) , (0.004 10 ± 0.000 38) , and (0.019 00 ± 0.003 53) (×10(-4) mm(3)/cycles) at 200 000, 400 000, 600 000, 800 000, 1 000 000, 1 200 000 cycles. The wear rates of heat-pressed ceramic were (0.139 50 ± 0.030 94), (0.124 40 ± 0.031 20), (0.054 80 ± 0.005 38), (0.038 80 ± 0.006 10), (0.011 10 ± 0.003 75), (0.198 90 ± 0.045 80) (×10(-4) mm(3)/cycles) at 200 000, 400 000, 600 000, 800 000, 1 000 000, 1 200 000 cycles. Three stages were observed in the wear loss process of the two materials: running-in stage, steady wear stage and severe wear stage. In running-in and steady wear stage, the shallow wear tracks of veneer porcelain were produced by the fatigue effect.While in severe wear stage, the wear tracks turned into ploughing. In running-in stage, the surface of heat-pressed ceramic was characterized by dense and shallow ploughing. In steady wear stage, the wear tracks turned into flake peeling produced by fatigue effect. At last, the whole layer was worn off by the effects of ploughing.</p><p><b>CONCLUSIONS</b>In a simulated oral environment, the wear rate and wear loss of heat-pressed ceramic are greater than that of veneer porcelain.</p>


Subject(s)
Analysis of Variance , Ceramics , Dental Porcelain , Dental Restoration Wear , Dental Veneers , Glass , Mastication , Microscopy, Electron, Scanning , Surface Properties
3.
Article in Chinese | WPRIM | ID: wpr-273513

ABSTRACT

<p><b>OBJECTIVE</b>To study the effects of monocyte-macrophages (THP-1) in malignant transformation of human bronchial epithelial cells (BEAS-2B) cells induced by coal tar pitch (CTP) and the expression of TNF-α in the process of the cell malignant transformation.</p><p><b>METHODS</b>BEAS-2B cells and THP-1 Cells were divided into four groups: coal tar pitch (CTP) group, benzo(a)pyrene [B(a)P] group, dimethyl sulfoxide (DMSO) group, BEAS-2B and THP-1 co-culture (co-culture group) group. Carcinogenesis model was established. The soft agar colony formation, chromosome aberrations and cell cycle tests were used to detect the cellular malignant transformation. The ELISA assay was utilized to measure the levels of TNF-α in the supernatant of CTP group and co-culture group.</p><p><b>RESULTS</b>The chromosome number abnormalities could be observed in early stage of the experiment (the 10th generation cells), which showed the increased ratio of aneuploid to polyploid, and the decreased number of diploid. The colony formation rate of co-culture group (the 20th generation cells) was 17.63‰ ± 0.97‰, which was significantly higher than that (13.94‰ ± 0.84‰) of CTP group and that (12.96‰ ± 1.62‰) of B(a)P group (P < 0.05). The proportion of S phase cells in the co-culture group was 44.49% ± 0.68%, which was significantly higher than that (38.19% ± 1.26%) of CTP group and that (36.41% ± 1.19%) of B(a)P group (P < 0.05). The TNF-α level in the co-culture group was significantly higher than that in CTP group (P = 0.001).</p><p><b>CONCLUSION</b>Monocyte-Macrophages can accelerate the malignant transformation of BEAS-2B cells induced by CTP and increase the expression level of TNF-α.</p>


Subject(s)
Bronchi , Cell Biology , Cell Line , Cell Transformation, Neoplastic , Coal Tar , Toxicity , Coculture Techniques , Epithelial Cells , Cell Biology , Pathology , Humans , Macrophages , Cell Biology , Monocytes , Cell Biology , Tumor Necrosis Factor-alpha , Metabolism
4.
Chinese Journal of Stomatology ; (12): 205-209, 2011.
Article in Chinese | WPRIM | ID: wpr-339772

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of different occlusal thickness and shoulder finish line depth on stress distribution of all-ceramic crowns and to select optimal occlusal thickness and shoulder finish line depth using continuous variation of parameters.</p><p><b>METHODS</b>This analysis was performed using mandibular first molar finite element model. The range of occlusal thickness was set from 1 mm to 3 mm, and that of shoulder finish line depth was from 0.4 mm to 1.2 mm. Load of 225 N was applied perpendicularly to the occlusal surface of the tooth at all buccal cusps to simulate functional occlusal force. The maximum equivalent stresses in crown, cement layer, abutment, periodontal ligament, alveolar bone were calculated, and the sensitivities of stresses to the variables were also evaluated.</p><p><b>RESULTS</b>The maximum equivalent stresses in crown, cement layer and abutment decreased as occlusal thickness and shoulder finish line depth were increased, while no obvious change were found in maximum equivalent stresses in periodontal ligament and alveolar bone. When occlusal thickness exceeded 1.87 mm and shoulder finish line depth exceeded 0.66 mm, the tangent slope rate of the maximum equivalent stress response curves ranged from -1 to 1. Data indicated that occlusal thickness played a more important role in reducing maximum equivalent stress in cement layer than finish line depth did, and shoulder finish line depth was a more effective parameter in reducing maximum equivalent stress in crown and abutment than occlusal thickness was.</p><p><b>CONCLUSIONS</b>Occlusal thickness exceeding 1.87 mm and shoulder finish line depth exceeding 0.66 mm are optimal design for ceramic crown on mandibular first molar from biomechanical point of view.</p>


Subject(s)
Ceramics , Chemistry , Crowns , Dental Cements , Chemistry , Dental Occlusion , Dental Prosthesis Design , Dental Stress Analysis , Finite Element Analysis , Humans , Mandible , Molar , Tensile Strength
5.
National Journal of Andrology ; (12): 348-350, 2011.
Article in Chinese | WPRIM | ID: wpr-266163

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the efficacy and safety of Tolterodine Tartrate combined with the alpha-receptor blocker in the treatment of benign prostatic hyperplasia with detrusor overactivity (BPH-DO).</p><p><b>METHODS</b>A total of 113 patients with BPH-DO were randomly assigned to receive Tolterodine Tartrate combined with Cardura (Group A) and Cardura alone (Group B), both for 12 weeks. Then we recorded and compared their average 24 h urinary frequency, IPSS and QOL score, maximum urinary flow rate, residual urine volume and urinary retention times before and after the treatment.</p><p><b>RESULTS</b>After the treatment, Group A showed significantly better improvement in the average 24 h urinary frequency and scores on IPSS and QOL than Group B. No significant differences were found between the two groups in the maximum urinary flow rate and residual urine volume. No acute urinary retention occurred in either group.</p><p><b>CONCLUSION</b>The combined use of Tolterodine Tartrate and the alpha-receptor blocker can effectively relieve the symptoms of dysuria, urinary frequency and urinary urgency in patients with BPH-DO, with neither significant adverse effects on the maximum flow rate and residual urine volume nor increase in the incidence of acute urinary retention.</p>


Subject(s)
Adrenergic alpha-Antagonists , Therapeutic Uses , Aged , Benzhydryl Compounds , Therapeutic Uses , Cresols , Therapeutic Uses , Humans , Male , Muscarinic Antagonists , Therapeutic Uses , Phenylpropanolamine , Therapeutic Uses , Prostatic Hyperplasia , Drug Therapy , Tolterodine Tartrate , Treatment Outcome , Urinary Bladder, Overactive , Drug Therapy
6.
Acta Pharmaceutica Sinica ; (12): 479-483, 2010.
Article in Chinese | WPRIM | ID: wpr-353371

ABSTRACT

To establish a bioassay method and quality standard of Banlangen granula, agglutinated activity assay was used in the analysis of the traditional Chinese medicine, Banlangen granula. It showed that masculined effect could be picked up effectively and the products quality of different pharmaceutical factories and different batch numbers from the same factory could be revealed conveniently, accurately, quickly and directly with this method (valence value was between 2 and 11). The established bioassay method had a good reproducibility with RSD = 2%. The dependablity of the activity of red cell agglutination and restrainting influenza virus NA was conspicuous (r2 = 0.878 3). In conclusion, this bioassay method is suitable to control and evaluate the quality of Banlangen granula. Thus the method may provide a simple and effective technique in supervising and examining the quality of other traditional Chinese medicine.


Subject(s)
Animals , Antiviral Agents , Pharmacology , Reference Standards , Biological Assay , Dosage Forms , Drugs, Chinese Herbal , Pharmacology , Reference Standards , Hemagglutination , Male , Neuraminidase , Metabolism , Orthomyxoviridae , Plants, Medicinal , Chemistry , Quality Control , Rabbits , Reproducibility of Results
7.
Acta Pharmaceutica Sinica ; (12): 535-538, 2010.
Article in Chinese | WPRIM | ID: wpr-353361

ABSTRACT

To establish kinetic assay method for the analysis of hemolysis and to investigate dynamic hemolytic process of polysorbate 80. The UV-VIS spectrum of heme changes when hemoglobin is released continuously during the hemolytic process. Therefore, dynamic hemolytic curve was determined as a new way to characterize the kinetic process of interaction between polysorbate 80 and red blood cells. The effect of polysorbate 80 on blood cells could be perfectly investigated by the hemolytic dynamics. Dynamic hemolytic parameters of polysorbate 80 were calculated according to the hemolytic curves. The constants of hemolytic rate and maximum hemolytic rate of polysorbate 80 had fine linear relationships at the range of 1-20 mg x mL(-1) and 5-20 mg x mL(-1), respectively. In comparison with the present official method such as macroscopic observation and static spectrophotometric methods, kinetic spectrophotometry has the advantages of real time, on-line determination, sensitive, objective, good reproducibility and 2-dimensional information acquired. Therefore, as a biological technique, kinetic spectrophotometry could be applied to evaluate the quality of polysorbate 80 and to screen other solubilizing excipients.


Subject(s)
Animals , Drug Stability , Erythrocytes , Chemistry , Excipients , Chemistry , Hemoglobins , Hemolysis , Kinetics , Polysorbates , Chemistry , Rabbits , Reproducibility of Results , Spectrophotometry, Ultraviolet , Methods
8.
Acta Pharmaceutica Sinica ; (12): 93-97, 2010.
Article in Chinese | WPRIM | ID: wpr-250614

ABSTRACT

This paper is aimed to provide the methods of quality control and bioassay of traditional Chinese medicine injections including bioassay method. Shuanghuanglian freeze-dried powder for injection (SFPI) was chosen as study object. HPLC-ELSD fingerprints of SFPI had been established and the samples were differentiated by similarity calculation. Meanwhile, biological profiles of SFPI on Escherichia coli had been established by microcalorimetry. The similarity values were calculated using the correlation coefficient, based on quantitative thermo-kinetic parameters (T2m, Tj, I%). The results indicated that HPLC-ELSD fingerprints, which showed content changes of chemical components, could not monitor minimal variation of different samples, especially that of biological pollutants, while biological profiles could sensitively detect antibiotic activity alterations of the samples, which were kept under specific conditions. In conclusion, characterized by two-dimension, microcalorimetry could supply thermograms as biological profiles characterized to describe the bioactivity of drugs. This study could clearly demonstrate that the correlative detection was proposed as an efficient strategy for quality control of SFPI, based on HPLC-ELSD fingerprints and biological profiles, which could detect quality fluctuation of samples early and quickly and predict the potential adverse drug events (ADE) for ensuring clinical safety.


Subject(s)
Calorimetry , Chromatography, High Pressure Liquid , Methods , Drug Combinations , Drugs, Chinese Herbal , Chemistry , Pharmacology , Escherichia coli , Freeze Drying , Injections , Light , Powders , Quality Control , Quantitative Structure-Activity Relationship , Scattering, Radiation
9.
Chinese Journal of Stomatology ; (12): 233-236, 2010.
Article in Chinese | WPRIM | ID: wpr-245219

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the reliability of all-ceramic crown on mandibular first molar under randomized biting force, and to explore the failure possibility of all-ceramic crown.</p><p><b>METHODS</b>Finite element (FE) models of all-ceramic crown on mandibular first molar were established using the Algor FE software. All-ceramic crown was loaded at different occlusal areas and positions with random variables of biting force, and the reliability of all-ceramic crown was analyzed.</p><p><b>RESULTS</b>The occlusal contact positions were located on the fossa, inclined surface and dental cusp. When the occlusal contact area was 0.4 mm(2), The reliability of all-ceramic crown on mandibular first molar were 100% (fossa), 99% (inclined surface), and 97% (cusp) respectively. When the occlusal contact area was 1.5 mm(2), the reliabilities of all-ceramic crown on mandibular first molar were 100% (fossa), 100% (inclined surface), and 99% (cusp) respectively. When the occlusal contact area was increased to 12.6 mm(2), the reliability of all-ceramic crown was 100%.</p><p><b>CONCLUSIONS</b>Premature occlusal contact and steep cusp inclination on all-ceramic crown on mandibular first molar will result in stress concentration and the crown will exhibit high failure probabilities. Chewing hard food is another contributing factor to the failure of all-ceramic crowns.</p>


Subject(s)
Bite Force , Ceramics , Chemistry , Crowns , Dental Stress Analysis , Finite Element Analysis , Humans , Mandible , Mastication , Molar , Prosthesis Failure , Reproducibility of Results , Tensile Strength
10.
Acta Pharmaceutica Sinica ; (12): 1136-1139, 2009.
Article in Chinese | WPRIM | ID: wpr-344043

ABSTRACT

A biothermal activity detection method has been established to determine the potency of colistin. The biothermal activity fingerprints of E. coli with colistin were determined. There was a good linear relationship (r = 0.993) between logarithm concentration of colistin (lgC) and lag rate of growing time (Deltat%) when the concentrations of colistin ranged from 17.0 to 41.6 u x mL(-1). The average recovery rate was 100.3% (n = 9). Using this method, there was no significant difference between results of colistin potency measurement and those using cup-plate method (P > 0.05). As a result, biothermal activity detection method is sensitive, accurate, rapid, convenient and feasible to determine the potency of colistin. This method can also be applied in real time and online to monitor the process of bacterial growth and could be complementary to the cup-plate method.


Subject(s)
Anti-Bacterial Agents , Pharmacology , Calorimetry , Methods , Colistin , Pharmacology , Dose-Response Relationship, Drug , Escherichia coli , Microbial Sensitivity Tests , Methods , Thermodynamics
11.
Chinese Journal of Stomatology ; (12): 752-753, 2008.
Article in Chinese | WPRIM | ID: wpr-346793

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the clinical performance of the crowns made by Cerec2 computer aided design and computer aided manufacture (CAD/CAM) system.</p><p><b>METHODS</b>Twenty four teeth were restored with Cerec2 CAD/CAM crowns. Eight years after delivery of the prostheses, the all-ceramic crowns were evaluated using modified standard of California Dental Association quality evaluation system. Patients filled out a satisfaction questionnaire at the same time. And Kaplan-Meier analysis was carried out.</p><p><b>RESULTS</b>Three out of 24 restorations were rated as failure. Kaplan-Meier analysis showed that the success rate of Cerec2 crowns eight year following delivery was 88%.</p><p><b>CONCLUSIONS</b>Crowns made by Cerec2 CAD/CAM system can survive in oral condition and function well for 8 years.</p>


Subject(s)
Adult , Computer-Aided Design , Crowns , Dental Porcelain , Dental Prosthesis Design , Female , Follow-Up Studies , Humans , Male , Middle Aged
12.
Article in Chinese | WPRIM | ID: wpr-318713

ABSTRACT

The aim of this study was to investigate the efficacy of diacetyl hexamethylene diamine (CAHB) for patients with high risk myelodysplastic syndrome (MDS), and to explore the effect of CAHB on HL-60 cells in vitro and its possible mechanism. 8 patients with high risk MDS were treated with CAHB by continuous intravenous infusion for 10 days, and repeated once after an interval of 28 days. The count of the granulo- and mono-blasts in bone marrow (BM) aspirate was measured before and after treatment. HL-60 cells were treated with different concentrations of CAHB for 72 hours in vitro. The inhibitory effect of CAHB on proliferation of HL-60 cells in vitro was measured by MTT assay. Differentiation of HL-60 cells was detected by the changes of CD11b and CD14 expression on cell surface. Apoptosis of HL-60 cells was detected by double staining of Annexin V and PI. The cell cycle distribution change of HL-60 cells was analyzed by flow-cytometry. The results indicated that the granulo- and mono-blasts in BM decreased in all the 8 patients after CAHB treatment. The main side effect of CAHB on hematological system was thrombocytopenia. After being treated with 1, 2, 3, 4 mmol/L CAHB for 72 hours in vitro, the result of MTT assay showed the inhibitory effect of CAHB on the proliferation of HL-60 cells in dose-dependent manner. After being treated manner 1, 2, 3, 4 mmol/L CAHB for 72 hours, the CD11b positive HL-60 cells were 22.39+/-3.97%, 33.12+/-4.46%, 49.25+/-5.27%, 78.05+/-5.66%, respectively, which were significantly different from the control group (CD11b positive HL-60 cells was 5.89+/-2.94%) (p<0.01). The CD14 expression was negative in all the 5 groups. These results suggested that CAHB could induce HL-60 cells to differentiate into mature granulocytes, and the effect of CAHB appeared in dose-dependent manner. After being treated for 72 hours by 1, 2, 3, 4 mmol/L CAHB, the apoptotic cells (Annexin V(+)/PI(-) cells) increased mildly, which suggested that CAHB only weakly induces HL-60 cells to apoptosis at the concentration of 1 to 4 mmol/L. Along with the concentration increase of CAHB, the ratio of cells in G(0)/G(1) phase increased, and ratio of cells in S phase and G(2)/M phase decreased correspondingly, it indicated that CAHB could arrest HL-60 cells in G(0)/G(1) phase in a dose-dependent manner. It is concluded that induction of cell differentiation may be the primary effect of CAHB on MDS. Cell cycle arrest may be essential to the effect of CAHB as well. Side effect of CAHB on platelet count may correlated with its inhibitory effect on hematopoiesis.


Subject(s)
Adult , Aged , Antineoplastic Agents , Therapeutic Uses , Apoptosis , Cell Proliferation , Cell Transformation, Neoplastic , Diamines , Therapeutic Uses , Female , HL-60 Cells , Humans , Male , Middle Aged , Myelodysplastic Syndromes , Drug Therapy
13.
Neuroscience Bulletin ; (6): 125-130, 2007.
Article in English | WPRIM | ID: wpr-300988

ABSTRACT

Current hypothesis of neuronal degeneration in Parkinson's disease (PD) have been proposed, including formation of free radicals and oxidative stress, mitochondrial dysfunction, excitotoxicity, trophic factor deficiency, inflammatory processes, genetic factors, environmental impact factors, toxic action of nitric oxide, apoptosis, and so on. This review mainly discussed oxidative stress, environmental impact factors, and inflammatory processes in PD.


Subject(s)
Animals , Environmental Pollutants , Humans , Inflammation , Allergy and Immunology , Oxidative Stress , Allergy and Immunology , Parkinson Disease , Allergy and Immunology
14.
Chinese Journal of Stomatology ; (12): 395-398, 2007.
Article in Chinese | WPRIM | ID: wpr-333310

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate mechanical behavior of different fixed prosthesis following molar hemisection therapy for clinical reference.</p><p><b>METHODS</b>Finite element method and photoelastic method were employed to investigate the stress distribution around the reserved distal root of the first mandibular molar with various fixed prosthesis designs. The restorations of single crown of the first mandibular molar, combined crowns of the first and second mandibular molars, combined crowns of the second mandibular premolar and first mandibular molar, and combined crowns of the second mandibular premolar, the first and second mandibular molars were designed. The same load was simulated on the occlusal surfaces of the molars.</p><p><b>RESULTS</b>The results of two studies showed that the stress around the reserved root in the single crown model was over 3 times as much as that of the normal model, the first mandibular molar with normal roots and a crown. For the combined crowns of the first and second mandibular molars, the stress was almost twice of the normal stress. While in the models of the combined crowns of the second mandibular premolar and first mandibular molar, as well as the combined crowns of the second mandibular premolar, the first and second mandibular molars, the stress distributions were similar to that in normal conditions.</p><p><b>CONCLUSIONS</b>Both the results indicated that the restoration of two-unit dual-ended combined crowns has favorable stress distribution and is applicable for the reserved root following the hemisection therapy.</p>


Subject(s)
Adult , Dental Prosthesis Design , Dental Stress Analysis , Denture, Partial, Fixed , Finite Element Analysis , Humans , Male , Mandible , Models, Anatomic , Molar
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