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1.
Acta Pharmaceutica Sinica ; (12): 533-540, 2022.
Article in Chinese | WPRIM | ID: wpr-922935

ABSTRACT

Hair roots induced by Agrobacterium rhizogene produce higher levels of secondary metabolites than non-induced plants, and the enhanced metabolic capacity is driven by the rol gene. We hypothesized that rol genes can be utilized to improve the biosynthesis of tropane alkaloids (TAs) in Atropa belladonna. In this study, the rolC gene from Agrobacterium rhizogene pRiA4 plasmid, driven by a CaMV35S promoter, was overexpressed in A. belladonna. The phenotypes, TAs content and transcriptional expression of key genes in TAs biosynthesis were analyzed in transgenic A. belladonna plants. Results show that transgenic A. belladonna exhibited a well-developed root system, male sterility, higher stamen column length than pistil, early flowering, internode shortening, smaller but more flowers, increased axillary buds and lateral buds, decreased apical dominance, and long and narrow leaves as compared to wild-type plants. Transgenic A. belladonna produced more TAs than wild-type plants, with the content of hyoscyamine, anisodamine and scopolamine reaching 2.58, 3.59 and 15.77-fold that of the control group, respectively. The gene expression of putrescine N-methyltransferase (PMT), tropinone reductase I (TRⅠ) and hyoscyamine 6-β-hydroxylase (H6H), key enzymes in TAs biosynthesis, were up-regulated compared with the control group. The above results indicate that the rolC gene enhances TAs biosynthesis in A. belladonna by up-regulating the expression of key enzymes in the TAs biosynthesis pathway, laying a foundation for genetic manipulation of A. belladonna to increase TAs content by increasing rolC gene expression.

2.
Article in Chinese | WPRIM | ID: wpr-921747

ABSTRACT

This study investigated the differential mechanisms of Rehmanniae Radix and Rehmanniae Radix Praeparata in improving diabetes in mice through AMPK-mediated NF-κB/NLRP3 signaling pathway. The diabetic mouse model was established with high-fat diet coupled with streptozotocin(STZ, intraperitoneal injection, 100 mg·kg~(-1), once a day for three consecutive days), after which the mice were randomly divided into model group, low-dose(5 g·kg~(-1)) and high-dose(15 g·kg~(-1)) Rehmanniae Radix groups, low-dose(5 g·kg~(-1)) and high-dose(15 g·kg~(-1)) Rehmanniae Radix Praeparata groups, catalpol group(250 mg·kg~(-1)), 5-hydroxymethylfurfural(5-HMF) group(250 mg·kg~(-1)), metformin group(250 mg·kg~(-1)), with the normal group also set. The organ indexes of heart,liver, spleen, lung, kidney and pancreas were calculated after four weeks of administration. The pathological changes and fibrosis of pancreas, kidney and liver in mice were observed by hematoxylin-eosin(HE) staining and Masson staining. Western blot was used to determine the expression levels of Toll-like receptor-4(TLR4), nuclear factor-κB(NF-κB), Nod-like receptor protein 3(NLRP3),interleukin-1β(IL-1β), adenosine monophosphate-activated protein kinase(AMPK), phosphorylated AMPK(p-AMPK) in the pancreas, kidney and liver of mice. Compared with the model group, the administration groups witnessed significant decrease in the liver,spleen, kidney, pancreas and fat indexes of diabetic mice, and there was no significant difference in heart and lung indexes. The pathological states and fibrosis of pancreatic, kidney and liver tissues were significantly improved after administration. Additionally, the expression levels of TLR4, NF-κB and NLRP3 in pancreas, kidney and liver of diabetic mice were significantly lowered. The expression levels of p-AMPK/AMPK were enhanced significantly in kidney and liver of mice in Rehmanniae Radix group while in pancreas, kidney and liver in Rehmanniae Radix Praeparata group. This suggests that Rehmanniae Radix and Rehmanniae Radix Praeparata differ in the mechanism of regulating energy metabolism of multiple organs and thereby exerting anti-inflammatory effects to alleviate symptoms of diabetic mice.


Subject(s)
AMP-Activated Protein Kinases/genetics , Animals , Diabetes Mellitus, Experimental/drug therapy , Diet, High-Fat/adverse effects , Mice , NF-kappa B/metabolism , NLR Family, Pyrin Domain-Containing 3 Protein , Plant Extracts , Rehmannia , Signal Transduction , Streptozocin
3.
Article in Chinese | WPRIM | ID: wpr-911566

ABSTRACT

Objective:To evaluate the clinical and ultrasonographic features for early diagnosis and prediction of lateral cervical lymph node metastasis of medullary thyroid microcarcinoma.Methods:From Jan 2010 to Jan 2020 233 patients undergoing primary surgery were categorized as "medullary thyroid microcarcinoma" and "medullary thyroid macrocarcinoma". The preoperative clinical and ultrasonographic characteristics, the diagnostic positive rate of preoperative serum calcitonin and fine needle aspiration (FNA) were investigated between two groups. All patients with medullary thyroid microcarcinoma were divided on the basis of wether there was lateral cervical lymph node metastasis.Results:There were statistically significant differences in initial diagnostic method(χ 2=32.290, P=0.000), TNM staging(χ 2=50.300, P=0.000) between medullary thyroid microcarcinoma and medullary thyroid macrocarcinoma. Medullary thyroid microcarcinoma showed more malignant ultrasonic features. The diagnostic accuracy of preoperative serum calcitonin was higher than FNA for medullary thyroid micro carcinoma(χ 2=47.933, P=0.000). Multivariate regression analysis demonstrated that the abutment/perimeter ≥1/4( OR=25.475, 95%CI: 2.320-279.771), preoperative serum calcitonin >65 ng/L( OR=32.663, 95%CI:2.433-438.409) were the independent factor for lateral cervical lymph node metastases of medullary thyroid microcarcinoma. Conclusions:The combination of ultrasonography and serum calcitonin helps establish early diagnosis of medullary thyroid microcarcinoma. Medullary thyroid microcarcinoma with the abutment/perimeter ≥1/4, serum calcitonin >65 ng/L predicts lateral cervical lymph node metastases.

4.
Article in Chinese | WPRIM | ID: wpr-910407

ABSTRACT

Objective:To validate the feasibility of establishing a dosimetric model for insect species by investigating the ecological environment of an inland site in the northwestern China.Methods:For Damalacore, a simplified anatomical model based on anatomy and geometry and a model based on CT scan sequence image were established to produce a voxel model. In combination with the Monte Carlo particle transport process, the deposited energy of the radionuclides in the insect tissues/organs was obtained. The dose rate from 90Sr and 137Cs to Damalacore was calculated on the basis of empirical formula. Results:The dose rate from internal exposure to the simplified anatomical model was 8.58×10 -2for 90Sr and 4.25×10 -3μGy/h for 137Cs, whereas the dose rate from external exposure to the simplified anatomy model was 2.81×10 -2for 90Sr and 2.56×10 -1μGy/h for 137Cs, respectively. The internal exposure to the voxel model from 90Sr and 137Cs was 3.91×10 -2and 2.91×10 -3μGy/h, whereas the external exposure to the voxel model from 90Sr and 137Cs was 2.81×10 -2 and 2.56×10 -1μGy/h, respectively. The internal exposure from 90Sr and 137Cs to ERICA model was 1.46×10 -1 and 1.46×10 -2μGy/h, whereas the external exposure to the ERICA model from 90Sr and 137Cs was 5.79×10 -2 and 2.58×10 -1μGy/h, respectively. Conclusions:The calculated results based on the two models are similar to those based on ERICA model and therefore are proved reliable. With improved model accuracy, the calculated result are more close to the practical situation and feasible.

5.
Article in Chinese | WPRIM | ID: wpr-907442

ABSTRACT

Follicular thyroid carcinoma(FTC) is a differentiated thyroid carcinoma originated from thyroid follicular cells. The sensitivity and specificity of ultrasound classification and scoring systems in diagnosis of follicular carcinoma are insufficient. Ultrasound guided fine needle aspiration(US-FNA) can screen but cannot diagnose FTC. Core needle biology(CNB) can reduce repeated FNA and unnecessary operations, and improve the diagnostic accuracy of FN patients, but CNB still has many controversies. Ultrasound guided FNA combined with gene detection (such as RAS) can significantly improve the diagnostic accuracy of follicular neoplasm. In recent years, artificial intelligence assisted diagnosis has shown high specificity in distinguishing FTC from follicular thyroid adenoma (FTA), which is conducive to the standardized treatment of thyroid follicular neoplasm. In this paper, the research progress of ultrasound based in diagnosis of FTC was reviewed.

6.
Article in Chinese | WPRIM | ID: wpr-907136

ABSTRACT

ObjectiveTo analyze the epidemiological characteristics of public health emergencies in Putuo District and provide scientific evidence to effectively respond to public health emergencies in the future. MethodsDescriptive epidemiological methods were used to analyze the data of public health emergencies reported by the national online system in Putuo District from 2004 to 2019. ResultsA total of 59 public health emergencies were reported from 2004 to 2019, during which 1 252 patients and 6 death cases were involved. From 2004 to 2019, the number of public health emergencies in Putuo District first increased and then decreased, fluctuating at a low level yearly. Most of the emergencies were general events (72.88%, 43/59). 69.49 percent (41/59) of the events were infectious diseases, and 67.80 percent (40/59) occurred in schools and child care institutes, with primary schools with the highest number of events. The peaks of infectious disease events occurred in June and November, while the majority of occupational poisoning and heat stoke events occurred in July and August, with 4 patients dead from heat stroke. The medians of events detection, verification, reporting and controlling timelines were 10.81, 3.00, 1.70 and 307.40 h; different categories of events varied in their detection and controlling time interval (all P<0.01). Multiple linear regression analysis showed that events detection and verification timelines were correlated with controlling timelines (b=0.766, 0.758, F=3.84, 5.65, all P<0.01). ConclusionsThe majority of public health emergencies in Putuo District are caused by infectious diseases, especially chicken pox. The prevention and control measures should be strengthened in schools. For occupational poisoning and heat stoke events, attention should be paid to different occupational groups. The capacity of emergency detection and verification should be enhanced to improve the efficiency of incident control.

7.
Article in Chinese | WPRIM | ID: wpr-907113

ABSTRACT

ObjectiveTo analyze the epidemiological characteristics of public health emergencies in Putuo District and provide scientific evidence to effectively respond to public health emergencies in the future. MethodsDescriptive epidemiological methods were used to analyze the data of public health emergencies reported by the national online system in Putuo District from 2004 to 2019. ResultsA total of 59 public health emergencies were reported from 2004 to 2019, during which 1 252 patients and 6 death cases were involved. From 2004 to 2019, the number of public health emergencies in Putuo District first increased and then decreased, fluctuating at a low level yearly. Most of the emergencies were general events (72.88%, 43/59). 69.49 percent (41/59) of the events were infectious diseases, and 67.80 percent (40/59) occurred in schools and child care institutes, with primary schools with the highest number of events. The peaks of infectious disease events occurred in June and November, while the majority of occupational poisoning and heat stoke events occurred in July and August, with 4 patients dead from heat stroke. The medians of events detection, verification, reporting and controlling timelines were 10.81, 3.00, 1.70 and 307.40 h; different categories of events varied in their detection and controlling time interval (all P<0.01). Multiple linear regression analysis showed that events detection and verification timelines were correlated with controlling timelines (b=0.766, 0.758, F=3.84, 5.65, all P<0.01). ConclusionsThe majority of public health emergencies in Putuo District are caused by infectious diseases, especially chicken pox. The prevention and control measures should be strengthened in schools. For occupational poisoning and heat stoke events, attention should be paid to different occupational groups. The capacity of emergency detection and verification should be enhanced to improve the efficiency of incident control.

8.
Article in Chinese | WPRIM | ID: wpr-906285

ABSTRACT

Objective:To explore the potential targets and mechanism of action of "Clematis Radix et Rhizoma-Trichosanthis Radix" based on network pharmacology. Method:Chinese Medicine System Pharmacology Analysis Platform(TCMSP) was used to screen out active ingredients and corresponding target proteins of Clematis Radix et Rhizoma and Trichosanthis Radix according to oral bioavailability(OB) and drug likeness(DL),cancer disease targets were screened out using GeneCards and OMIM databases,R language software was used to screen out common targets of clematis,trichosanthin and cancer diseases, Cytoscape 3.7.2 software was used to construct a network map of "drug-active ingredient-disease-target", STRING database was used to draw protein protein interaction(PPI)of common target proteins, R language software was used to perform enrichment analysis of gene ontology(GO) functions and Kyoto encyclopedia of genes and genomes(KEGG) channels on effective targets. Result:A total of 9 effective active ingredients were obtained from Clematis Radix et Rhizoma-Trichosanthis Radix powder pair. A total of 31 target genes were searched,and 814 relevant target genes were searched from cancer diseases. The two kinds of relevant target genes were matched to obtain 9 common target genes,which mainly involved endopeptidase,cysteine-type endopeptidase activities involving in the apoptosis process and cancer necrosis factor receptor superfamily binding and other biological processes,and played a role in the treatment of cancers by regulating apoptosis,measles,hepatitis B,kaposi sarcoma-associated herpesvirus infection,p53,interleukin-17(IL-17),tumor necrosis factor(TNF) and many other pathways. Conclusion:The mechanism of Clematis Radix et Rhizoma-Trichosanthis Radix in the treatment of cancer is preliminarily studied. Clematis Radix et Rhizoma-Trichosanthis Radix has multiple active ingredients and can play a role in treating cancer through multiple targets and multiple pathways.

9.
Article in Chinese | WPRIM | ID: wpr-906255

ABSTRACT

Objective:The Meta-analysis was used to systematically evaluate the clinical efficacy of traditional Chinese medicine in treating Late onset hyponatremia (late onset hyponatremia,LOH). Method:Pubmed,Web of Science,China Knowledge Base Database (CNKI),Wanfang Database (WanFang),Weipu Full-text Periodical Database(VIP),Chinese Biomedical Literature Database (CBM)were retrieved to collect randomized controlled trials of traditional Chinese medicine(TCM) for treatment of LOH. Two researchers independently screened out the literatures, extracted the data, conducted quality assessment by Cochrance bias risk assessment tool,and made Meta-analysis by RevMan 5.3.5 software. Result:Nine eligible documents were finally included. Meta-analysis results showed that the test group was superior to the control group in improving patient's physical fitness/cardiovascular score [mean deviation(MD)=-1.42,95% CI(-2.39,-0.45),<italic>P</italic>=0.004] and psycho-psychological score[MD=-0.74,95% CI(-1.26,-0.22),<italic>P</italic>=0.005],with no statistically significant difference between test group and control group in sexual function score [MD=-0.68,95% CI(-1.38,-0.03),<italic>P</italic>=0.06],serum testosterone (TT) concentration[MD=-0.68,95% CI(-1.38,-0.03),<italic>P</italic>=0.06] and effective rate [odds ratio(OR)=1.57,95% CI(0.64,3.88),<italic>P</italic>=0.33]. Conclusion:TCM is equivalent to western medicine(testosterone undecanoate)in the treatment of late onset hypogonadism, and better than western medicine in improving patients' physical fitness/cardiovascular score and mental and psychological score.

10.
Article in Chinese | WPRIM | ID: wpr-906223

ABSTRACT

In China, about 100 million people currently have chronic obstructive pulmonary disease (COPD). At the same time, COPD is a multisystem disease, not only affecting the function of musculoskeletal, cardiovascular, kidney and immune systems in patients, but also causing intestinal dysfunction as its extrapulmonary manifestations. From the perspective of traditional Chinese medicine (TCM), after COPD is formed, deficiency, phlegm stasis and toxicity were accumulated in the lungs, which leads to dysfunction of lung in dispersing and descending, and eventually causes ascending and descending disorder of Qi activities, disorder of fluid supply and distribution, and stagnation of blood stasis. The viscera disease would affect the bowels, and the large intestine is thus affected. Modern medical discovers that, the lungs and intestines have common origins and similar physiological structures, in pathological circumstances, their common mucosal immune system may lead to similar immune factors and inflammatory manifestations in the lungs and intestines. At the same time, the studies have confirmed that there is also a close relationship between intestinal flora and lung, that is "lung-gut axis". These theories partially illustrate the mechanism of COPD in inducing intestinal injury. The specific manifestations of COPD intestinal dysfunction, ① Flora disorder, with increased abundance of intestinal gram-negative bacilli, and inhibited reproduction of Bifidobacterium, Lactobacillus and short-chain fatty acid-producing bacteria. ② Intestinal barrier damage: characterized by the destruction of intestinal epithelium tight connectivity, increased intestinal permeability, and thinning of the mucus layer. ③ Intestinal motility disorder: mostly manifested as weight loss and malnutrition. At present, for the intestinal dysfunction in COPD patients, most of the relevant discussions and targeted treatment methods in TCM are scattered and unsystematic. Guided by the idea of treating different diseases with the same treatment, we summarized the etiology and pathogenesis of COPD intestinal dysfunction by learning from the experience of TCM in treating intestinal flora disorders and inflammatory bowel disease, and proposed preliminary formulation with Tiaoqi Qushi,Tongfu Tongluo as its basic treatment principles in this paper, hoping to provide new ideas for the treatment of COPD.

11.
Article in Chinese | WPRIM | ID: wpr-906067

ABSTRACT

Objective:To explore the therapeutic principles and medication regularity of Chinese medicine for the treatment of urolithiasis based on data mining and literature research. Method:Chinese National Knowledge Infrastructure(CNKI) was searched for the clinical research papers on Chinese medicinal compounds for the treatment of urolithiasis published from database inception to November 10,2020. Therapeutic methods and Chinese medicinal compounds were extracted from the included papers,and the frequencies of therapeutic methods and the drugs in the Chinese medicinal compounds,as well as the efficacies were analyzed. The medication regularity of high-frequency drugs was explored by association rule analysis and cluster analysis. Result:A total of 247 papers and 247 Chinese medicinal compounds were included,and 209 drugs were involved. Those with high frequencies included Lysimachiae Herba,Galli Gigerii Endothelium Corneum,Lygodii Spora,Achyranthis Bidentatae Radix,Pyrrosiae Folium,Plantaginis Semen,and Talcum. The drugs with the highest frequency were effective in promoting urination,draining dampness,and activating blood to resolve stasis,followed by those in tonifying deficiency,regulating Qi,and purgation. Galli Gigerii Endothelium Corneum,Clematidis Radix et Rhizoma,and Succinum were used as specialized drugs targeting the syndrome. Association rule analysis showed that Lysimachiae Herba,Galli Gigerii Endothelium Corneum,and Lygodii Spora were commonly used in pairwise or ternary combination,followed by the pairwise combinations of Lysimachiae Herba with Achyranthis Bidentatae Radix,Pyrrosiae Folium,and Plantaginis Semen. High-frequency drugs were clustered into four categories by clustering analysis. Thirty-one therapeutic principles were employed in the 247 Chinese medicinal compounds. The ones with the highest frequency were clearing heat and eliminating dampness,resolving stasis and promoting urination,and tonifying kidney and replenishing qi. Conclusion:For the treatment of urolithiasis,promoting urination and draining dampness is the core therapeutic principle,and activating blood to resolve stasis is the important therapeutic principle,while tonifying is the potential therapeutic principle. The medication for urolithiasis is featured by specialized drugs targeting the syndrome.

12.
Article in Chinese | WPRIM | ID: wpr-905846

ABSTRACT

Objective:To study the distribution of ischemic stroke treatment with data mining technology and evaluate its clinical efficacy. Method:China National Knowledge Infrastructure Database(CNKI),China Science and Technology Reader's Digest Database(VIP),Wanfang Data,Chinese Biomedical Literature Database(Sino Med)were retrieved from January 1978 to December 2018. The clinical observation and study literatures on the treatment of ischemic stroke with the combination of traditional Chinese medicine and Western medicine were retrieved in the four databases. After standardized and hierarchical collection and processing of all syndromes,treatment methods,prescriptions and other information in the literatures,a database of syndrome elements and treatment of ischemic stroke was established. Syndrome factors and treatment methods were analyzed by scale evaluation and hierarchical classification methods. Kendall's tau-b correlation analysis,principal component analysis and other statistical methods were used to describe the correlation and distribution of syndrome factors and treatment methods of ischemic stroke. Result:The results of heterogeneity analysis showed that the included literatures were homogeneous and could be combined with subsequent statistics. A total of 450 syndromes and treatment methods were included in this study,and 1 287 single syndrome elements and 1 562 single treatment methods were obtained after unified and standardized splitting. Besides the corresponding syndrome elements and treatment methods,phlegm-dampness-invigorating Qi(-0.52) and Qi deficiency-invigorating Qi(-0.56) were also highly correlated. The study team represented the importance of syndrome and treatment elements with class Ⅰ,Ⅱ,Ⅲ from high to low. Qi deficiency,blood stasis and fire heat,phlegm,viscera excess were class Ⅰ syndrome elements;Yin deficiency,endogenous wind were class Ⅱ syndrome elements;Yin deficiency and Yang deficiency were class Ⅲ syndrome elements;Removing phlegm dampness,clearing heat,clearing the hollow viscera and extinguishing wind,promoting blood circulation to remove blood stasis,tonifying Qi were class Ⅰ treatment of ischemic stroke,and removing phlegm dampness,clearing heat,clearing the hollow viscera were more likely to appear simultaneously; and extinguishing wind,activating blood circulation and removing blood stasis,and benefiting Qi were more likely to appear simultaneously. Nourishing Yin and regulating Qi were class Ⅱ therapies of ischemic stroke,which were highly correlated and often appear simultaneously. Inducing resuscitation,tonifying Yang and dredging collaterals were class Ⅲ,Ⅳ,Ⅴ therapies. Conclusion:Qi deficiency,blood stasis,phlegm dampness,fire heat and viscera excess were the main syndromes of ischemic stroke,while Qi deficiency and blood stasis,phlegm heat and viscera excess were the main syndromes. Eliminating phlegm and dampness,clearing heat,clearing the hollow viscera,promoting blood circulation and removing blood stasis,extinguishing wind and benefiting Qi were the main therapies for the treatment of ischemic stroke. In clinical treatment for ischemic stroke,the therapies for relieving phlegm and dampness,clearing heat and relieving organs are often used in combination,and the therapies for promoting blood circulation and removing blood stasis were often used in combination with the therapies for invigorating Qi and extinguishing wind for the synergistic effect.

13.
Article in Chinese | WPRIM | ID: wpr-905254

ABSTRACT

Objective:To construct a teamwork model, Partnership Rehabilitation Therapy (PRT), for therapists in critical wards for patients with Corona Virus Disease 2019 (COVID-19), and observe its effect. Methods:PRT had been developed, in which one therapist (main) implementing therapy and another (assistant) monitoring and supporting in the treatment. Eleven COVID-19 patients from infectious critical ward were treated with PRT. The behavior safety of therapists was recorded during the treatment, and the patients were assessed with Borg Index, Cough Score, Miller Sputum Grading and World Health Organization Disability Assessment Schedule (WHODAS) 2.0 before and after treatment. Results:No physiotherapist was infected by COVID-19. Seven times of infection risks were recorded and avoided, and six times of treatment risks were corrected instantly. All the patients improved in Borg Index (P < 0.01), Cough Score (P < 0.05), Miller Sputum Grading (P = 0.02) and WHODAS 2.0 (P < 0.01) after a 1-week physical therapy. Conclusion:Based on the Family International Classifications, a teamwork model is established, which provides a safe and practicable way for rehabilitation for COVID-19 patients in critical wards.

14.
Article in Chinese | WPRIM | ID: wpr-905211

ABSTRACT

Objective:To observe the relationship of ventilatory efficiency to cardiac function, especially heart rate recovery after exercise for patients with chronic obstructive pulmonary disease (COPD). Methods:From January, 2019 to December, 2020, 190 patients with COPD were recruited for Cardiopulmonary Exercise Testing. The general condition, medical history and medication history, lung function test and parameters of Cardiopulmonary Exercise Testing were recorded. They were divided into normal group and delay group according to whether the heart rate decline more than twelve beats within a minute after Cardiopulmonary Exercise Testing. Results:There were 89 patients (46.84%) in the delay group. Compared with the normal group, the delay group were older (Z = 2.282, P < 0.05), with less ratio of force exiratory volume in the first second in prediction (FEV1.0%) (Z = 3.626, P < 0.001), maximum power (t = 5.547, P < 0.001), breath reserve (BR) (t = 2.122, P < 0.05) and higher minimum ventilation equivalent of carbon dioxide (VE/VCO2nadir) (Z = 3.296, P = 0.001). Logistic regression showed that the COPD severity, VE/VCO2nadir and BR correlated with heart rate recovery. After adjusting for gender, age, body mass index and COPD severity, VE/VCO2nadir was an independent risk factor for delayed heart rate recovery (OR = 1.203, 95%CI 1.032 to 1.873, P = 0.004), and the best cut-off point was 33.15 (AUC = 0.6387, 95%CI 0.5595 to 0.7178, P = 0.001). Conclusion:The ventilatory inefficiency may increase the risk of abnormal heart rate recovery after exercise in COPD patients.

15.
Organ Transplantation ; (6): 767-2021.
Article in Chinese | WPRIM | ID: wpr-904563

ABSTRACT

Posttransplant lymphoproliferative disease (PTLD) is a series of heterogeneous lymphoproliferative diseases and a severe complication after solid organ transplantation in children. Over 70% of PTLD is associated with Epstein-Barr virus (EBV). EBV-related B-cell lymphoma is also the main malignant tumor after pediatric organ transplantation. EBV-related PTLD is still a challenge in pediatric solid organ transplantation, which is mainly caused by immune function damage induced by immune suppression after transplantation. However, the specific mechanism remains elusive. In recent years, biomarkers have been developed to guide the diagnosis and individualized treatment of EBV-related PTLD, which possesses excellent application prospect. In this article, research progresses on the incidence of EBV-related PTLD in solid organ transplantation and its biomarkers were reviewed, aiming to explore novel ideas for clinical diagnosis and treatment.

16.
Article in English | WPRIM | ID: wpr-888778

ABSTRACT

Two new lignan glucosides, tinsinlignans A and B (1 and 2), two new oxyneolignans, tinsinlignans C and D (3 and 4), along with one known analogue (5), were isolated from the stems of Tinospora sinensis. The structures of the new compounds were elucidated based on analysis of spectroscopic data, and the absolute configuration of 1 was determined through electronic circular dichroism (ECD) calculation based on the time-dependent density functional theory (TD-DFT). Compounds 1-4 were evaluated for their inhibitory effects on nitric oxide (NO) production induced by lipopolysaccharide (LPS) in murine RAW264.7 macrophage cells and compounds 1 and 2 exhibited moderate inhibitory activities with IC


Subject(s)
Animals , Glucosides/pharmacology , Lignans/pharmacology , Lipopolysaccharides , Mice , Molecular Structure , Nitric Oxide , Phytochemicals/pharmacology , Tinospora/chemistry
17.
Chinese Medical Journal ; (24): 1299-1309, 2021.
Article in English | WPRIM | ID: wpr-878164

ABSTRACT

BACKGROUND@#Bendamustine was approved in China on May 26th, 2019 by the National Medical Product Administration for the treatment of indolent B-cell non-Hodgkin lymphoma (NHL). The current study was the registration trial and the first reported evaluation of the efficacy, safety, and pharmacokinetics of bendamustine in Chinese adult patients with indolent B-cell NHL following relapse after chemotherapy and rituximab treatment.@*METHODS@#This was a prospective, multicenter, open-label, single-arm, phase 3 study (NCT01596621; C18083/3076) with a 2-year follow-up period. Eligible patients received bendamustine hydrochloride 120 mg/m2 infused intravenously on days 1 and 2 of each 21-day treatment cycle for at least six planned cycles (and up to eight cycles). The primary endpoint was the overall response rate (ORR); and secondary endpoints were duration of response (DoR), progression-free survival (PFS), safety, and pharmacokinetics. Patients were classified according to their best overall response after initiation of therapy. Proportions of patients in each response category (complete response [CR], partial response [PR], stable disease, or progressive disease) were summarized along with a two-sided binomial exact 95% confidence intervals (CIs) for the ORR.@*RESULTS@#A total of 102 patients were enrolled from 20 centers between August 6th, 2012, and June 18th, 2015. At the time of the primary analysis, the ORR was 73% (95% CI: 63%-81%) per Independent Review Committee (IRC) including 19% CR and 54% PR. With the follow-up period, the median DoR was 16.2 months by IRC and 13.4 months by investigator assessment; the median PFS was 18.6 months and 15.3 months, respectively. The most common non-hematologic adverse events (AEs) were gastrointestinal toxicity, pyrexia, and rash. Grade 3/4 neutropenia was reported in 76% of patients. Serious AEs were reported in 29 patients and five patients died during the study. Pharmacokinetic analysis indicated that the characteristics of bendamustine and its metabolites M3 and M4 were generally consistent with those reported for other ethnicities.@*CONCLUSION@#Bendamustine is an active and effective therapy in Chinese patients with relapsed, indolent B-cell NHL, with a comparable risk/benefit relationship to that reported in North American patients.@*CLINICAL TRIAL REGISTRATION@#ClinicalTrials.gov, No. NCT01596621; https://clinicaltrials.gov/ct2/show/NCT01596621.


Subject(s)
Adult , Antineoplastic Combined Chemotherapy Protocols , Bendamustine Hydrochloride/therapeutic use , China , Humans , Lymphoma, Non-Hodgkin/drug therapy , Neoplasm Recurrence, Local/drug therapy , Prospective Studies , Rituximab/therapeutic use
18.
Chinese Journal of School Health ; (12): 611-614, 2021.
Article in Chinese | WPRIM | ID: wpr-876414

ABSTRACT

Objective@#To understand the prevalence of obesity and its associations with diet,exercise and mental health among students in Inner Mongolia, so as to provide scientific basis for the formulation of obesity intervention measures for students in this area.@*Methods@#Stratified and cluster random sampling was used to select 186 649 students from all 12 cities and 103 banners and counties in Inner Mongolia Autonomous region. All the participants were divided into groups according to the BMI classification criteria for overweight/obesity screening of Chinese school age children and adolescents (kg/m 2). Univariate analysis was carried out by means of rectangular χ 2 test, and multivariate unconditional Logistic regression model was used to screen the risk factors of obesity.@*Results@#The overall obesity rate was 18.54%, the obesity rate of boys was 22.04 %, and the obesity rate of girls was 14.99%. The results of multivariate Logistic regression analysis showed that area(OR=0.79), sex (OR=0.61), times of eating fried food per day (OR=1.08), daily exercise for more than 60 minutes per week >2 days (OR=0.89),eat breakfast every day (OR=0.86), times of drinking drinks per day (OR=1.12), Smoking (OR=0.87), drinking (OR=1.07), eating fruit per day (OR=1.07), watching TV ≥2 hours a day (OR=1.06), sleeping time (OR=0.93) and school period may be related to obesity (P<0.05).@*Conclusion@#The overall obesity rate of students in Inner Mongolia is relatively high, and the occurrence of obesity is related to diet, exercise behavior, living habits and psychological factors.

19.
Acta Pharmaceutica Sinica ; (12): 2182-2196, 2021.
Article in Chinese | WPRIM | ID: wpr-887038

ABSTRACT

Bioisosterism is one of the most common strategies in drug structure optimization. With the development of medicinal and organic chemistry, more and more classic and non-classical bioisosteres are used in the design of novel drugs. In recent years, fluorinated groups as a bioisostere have been paid more and more attention by pharmaceutical chemists. This paper briefly reviews the physicochemical properties, chemical preparation methods of difluoromethyl (CF2H) group, and its application in drug design to provide references for drug discovery researchers.

20.
Article in Chinese | WPRIM | ID: wpr-884598

ABSTRACT

Epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) has become the standard first-line treatment for advanced non-small cell lung cancer (NSCLC) patients with EGFR-sensitive mutations. However, acquired resistance cannot be avoided during the targeted treatment of EGFR-TKI, which may lead to the progression of NSCLC. Among them, approximately 50% of patients present with primary lung lesions and/or initial metastastic lesion progression. As a major local treatment, radiation therapy plays an irreplaceable role in the management of patients with advanced cancer. Stereotactic body radiation therapy (SBRT), which is implemented with the Cyberknife, is the most advanced radiation therapy technology to better meet clinical needs. In recent years, based on the abovementioned patterns of tumor recurrence, the treatment mode of pulmonary-lesion SBRT combined with EGFR-TKI can improve the tumor-free survival in advanced NSCLC patients, which has attracted widespread attention. In this article, the clinical research progresses on this combined therapeutic strategy were reviewed.

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