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1.
Acta Pharmaceutica Sinica ; (12): 1470-1477, 2021.
Article in Chinese | WPRIM | ID: wpr-887080

ABSTRACT

In recent years, the overexpression dopamine (DA) due to the use of addictive drugs has caused concern and urgently needs to be addressed. The method used in our study is known as biomimetic sol-gel synthesis. We undertook the experiment to develop molecularly imprinted xerogel polymers (MIXPs) through template molecules dopamine polymerized with polyethyleneimine (PEI), then self-assembled and crosslinked with tetramethoxysilane (TMOS) in the form of non-covalent hydrogen bonds by using biomimetic sol-gel process, and then eluted template DA will leave a blotting site. Monoamine oxidase immobilized MIXPs (MAO-MIXPs) was obtained by coating monoamine oxidase onto MIXPs. The synthesis optimization of MAO-MIXPs was finally set as the ratio of DA template, PEI and MAO coating (DA 40 mg, PEI 0.6 mL, MAO 2.5 mg·g-1) to achieve highly selective adsorption toward DA in artificial cerebrospinal fluid based on the adsorption performance and degradation performance. The micromorphologies and physical-chemical properties of MAO-MIXPs were characterized by scanning electron microscopy, differential scanning calorimeter and Fourier transform infrared spectroscopy, and then amount of adsorption was calculated with adsorption equation. Simultaneously, the Brunner-Emmet-Teller (BET) and Langmuir model were simulated. It was found that the adsorption behavior tended to be monolayer adsorption. This new molecularly imprinted polymer demonstrated potential dopamine expression regulation for highly selective recognition, adsorption and degradation of dopamine.

2.
Article in Chinese | WPRIM | ID: wpr-872991

ABSTRACT

Objective::To observe the clinical efficacy of modified Simiaosan on synovitis of knee joint with damp-heat obstruction collateral syndrome and the effect on inflammatory factors and neuropeptide substances in synovial fluid. Method::One hundred and thirty-five patients were randomly divided into control group(67 cases) and observation group(68 cases) by random number table. Patients' intra-articular effusion was drawn out. Triamcinolone acetonide was injected into arthrosis for 2 times, 1 time/week, and aceclofenac sustained-release tablets were given for 4 weeks, 0.2 g/time, 1 time/day. In addition to the therapy of control group, patients in observation group were also given modified Simiaosan for 4 weeks, 1 dose/day. Before and after treatment, pain, swelling of knee joint and were scored, Western Ontario and McMaster University (WOMAC) were adopted for scoring knee score, and damp-heat obstruction syndrome and local signs of knee joint were also assessed. And levels of interleukin-1 beta (IL-1 beta), IL-6, tumor necrosis factor-alpha (TNF-alpha), hypersensitive C-reactive protein (hs-CRP), substance P (SP), calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY) and vasoactive intestinal peptide (VIP) were detected. Result::After treatment, scores of pain and swelling of knee joint in observation group were lower than those in control group (P<0.01). Scores of WOMAC, the total score, integral of damp-heat obstruction collateral syndrome, knee flexion-extension range score and floating patella test were all lower than those in control group (P<0.01). And levels of IL-1β, IL-6, TNF-α, hs-CRP, SP, CGRP, NPY and VIP were all lower than those in control group (P<0.01). Analyzed by rank sum test, the clinical efficacy in observation group was better than those in control group (Z=2.089, P<0.05). Conclusion::Modified Simiaosan can significantly alleviate pain and swelling symptoms, promote the recovery of knee joint function, and inhibit the expressions of proinflammatory factors and neuropeptides in synovial fluid, with a better clinical efficacy.

3.
Article in Chinese | WPRIM | ID: wpr-701191

ABSTRACT

Transcription factor EB(TFEB)is a member of the MiTF/TFE family and plays an important role in cell stress,metabolism,cancer and so on.There are relatively few studies on the role of TFEB in renal diseases.TFEB was initially found to be highly expressed in TFEB-fusion renal cell carcinoma and plays a key role in the development of re-nal cell carcinoma.Blocking the downstream signaling pathway activated by TFEB would be a promising treatment for TFEB-fusion renal cell carcinoma.On the contrary,the expression of TFEB in renal intrinsic cells is decreased in diabetic kidney disease,leading to a blockage in the autophagy-lysosome pathway.TFEB enhances the ability of cell stress and self-repair,and then delays the progress of diabetic kidney disease.In cystine nephropathy,TFEB expression is reduced in re-nal tubular epithelial cells and compensatory activation is insufficient as well.TFEB over-expression effectively eliminates intracellular cystine and repairs damaged lysosome,which is expected to alleviate or cure the Fanconi syndrome.In summa-ry,TFEB plays a key role in different kidney diseases,and targeted regulation of TFEB provides new hope for the treatment of kidney diseases.

4.
Article in Chinese | WPRIM | ID: wpr-690402

ABSTRACT

In order to obtain the optimum method for content determination of Forsythia Fructus (FF), a variety methods for the sample preparation of FF were evaluated by the content determination methods of Chinese Pharmacopoeia. And an optimum method was screened and as follows: 30 times with 70% ethanol solution in ultrasonic extractor for half an hour. The method can achieve the best effect of simultaneously extracting forsythoside A and forsythin. Then, a HPLC method for simultaneous determination of forsythoside A and forsythin was established by methodology. The HPLC chromatographic conditions: the mobile phase consisted of acetonitrile (A)-0.4% acetic acid solution (B) with gradient elution [0-33 min,15%A,33-43 min,15%-25%A,43-60 min,25% A] was at the flow rate of 1 mL·min⁻¹, the column temperature was 25 °C, and the detection wavelength was 330 and 277 nm. Moreover, the contents of forsythoside A and forsythin for 10 Green Forsythia Fructus (GF) and 5 Old Forsythia Fructus (OF) were determined by this method and Chinese Pharmacopoeia. The result not only displayed that the established method is effective, rapid, and simple, but also showed that the contents of forsythoside A and forsythin for GF and OF were significantly different. Which implied that the forsythoside A and forsythin limit standard for GF and OF should be controled by different values. This studies provide an important basis for the establishment of the content determination of FF and the quality control standard for GF and OF.

5.
Journal of Experimental Hematology ; (6): 1553-1558, 2018.
Article in Chinese | WPRIM | ID: wpr-689898

ABSTRACT

<p><b>OBJECTIVE</b>To study the coagulation properties the refrigerated whole blood stored at 4℃.</p><p><b>METHODS</b>Ten units of whole blood were obtained from healthy volunteer donors and stored at 4±2℃ for 21 days. Samples were collected on the day after donation and on days 2, 4, 6, 8, 10, 14 and 21 for delection including complete blood count, electrolyte, APTT, PT, Fg, blood coagulation factors, and thromboelastography(TEG).</p><p><b>RESULTS</b>The levels of Hb, WBC, Plt, sodium and potassium in each sample accorded with standard of storing whole blood. The level of Hb, WBC, Plt and Na decreased along with prolonging of storage time, while the K level increased along with prolonging of stored time, APTT and PT prolonged along with prolonging of thored time, PT>17 min at d 21, the Fg level change was no-obvious, The level of factor Ⅴ and Ⅷ decreased more than 50 % of baseline on d 6 and 4 respectively; the levels of factor Ⅱ, Ⅶ, Ⅸ, Ⅹ, Ⅺ, Ⅻ showed decreasing trend, but their levels were less than 40 % of baseline values at d 21. TEG test showed that no abnormalily of R value was found, the abnormal valnes of K and Angle were observed at d 21, the abnormal value of MA was observed at d 14.</p><p><b>CONCLUSION</b>The whole blood stored for 10 days possesses normal coagulation function showing important significance for treatment of hemorrhage from war injury and surgical openation of heart and chest.</p>

6.
Acta Pharmaceutica Sinica ; (12): 133-140, 2015.
Article in Chinese | WPRIM | ID: wpr-251805

ABSTRACT

Chronic obstructive pulmonary disease (COPD), a common preventable and treatable disease, is characterized by airflow limitation that is usually progressive and associated with an enhanced chronic inflammatory response in the airways. Its main pathological manifestations include airway inflammation, mucus hypersecretion, oxidative stress and apoptotic epithelial cells. Recent research suggests that MAP kinases and Keap1-Nrf2-ARE signaling pathway are involved in the pathological process of inflammation and oxidative stress. This review explores the potential role of the cross talk of these signaling pathways in airway inflammation, mucus hypersecretion, oxidative stress and apoptotic epithelial cells. To clarify the roles of cross talk between MAP kinases and Keap1-Nrf2-ARE signaling pathway, we also focus on the drugs related to that in the treatment of COPD, and it provides ideas for more drug research in the treatment of COPD.


Subject(s)
Apoptosis , Epithelial Cells , Cell Biology , Humans , Inflammation , Intracellular Signaling Peptides and Proteins , Kelch-Like ECH-Associated Protein 1 , Mitogen-Activated Protein Kinases , NF-E2-Related Factor 2 , Oxidative Stress , Pulmonary Disease, Chronic Obstructive , Metabolism , Respiratory System , Signal Transduction
7.
Article in Chinese | WPRIM | ID: wpr-236381

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of chloride channel blocker--niflumic acid (NFA) on the pathological process of hypoxia hypercapnia-induced pulmonary vasoconstriction in rats.</p><p><b>METHODS</b>We used the model of hypoxia hypercapnia-induced pulmonary vasoconstriction rats, and divided the second, third branch pulmonary artery rings randomly into four groups (n = 8): control group (N group), hypoxia hypercapnia group (H group), DMSO incubation group (HD group), niflumic acid group (NFA group). Under acute hypoxia hypercapnia conditions, we observed the effects of the three stages of hypoxia hypercapnia-induced pulmonary vasoconstriction (HHPV) incubated by NFA in the second, third brach pulmonary artery rings. At the same time, the values of rings' tension changings were recorded via the method of hypoxia hypercapnia conditions reactivity. And investigated the effect of NFA to HHPV.</p><p><b>RESULTS</b>(1) Under the hypoxia hypercapnia condition, we observed a biphasic pulmonary artery contractile (the phase I rapid contraction and vasodilation; the phase II sustained contraction) response in both the second and the third branch pulmonary artery rings compared with the control group (P < 0.05 , P < 0.01); (2) The second and third pulmonary artery rings incubated by NFA which phase II persistent vasoconstriction were significantly attenuated compared with the H group (P < 0.05 , P < 0.01).</p><p><b>CONCLUSION</b>The blocker of the chloride channels attenuates the second and third branch pulmonary artery rings constriction in rat, especially the phase II persistent vasoconstriction, so then have an antagonistic effect on HHPV.</p>


Subject(s)
Animals , Chloride Channels , Hypercapnia , Hypoxia , Niflumic Acid , Pharmacology , Pulmonary Artery , Pulmonary Circulation , Rats , Vasoconstriction
8.
Article in Chinese | WPRIM | ID: wpr-236372

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the role and significance of ATP-sensitive K+ channels in the pathological process of hypoxia hypercapnia-induced pulmonary vasoconstriction (HHPV) and the relationship with ERK1/2 signal pathway in rats.</p><p><b>METHODS</b>We made the third pulmonary artery rings of SD rats, used the model of pulmonary artery rings perfusion in vitro. Under acute hypoxia hypercapnia condition, and observed the effects of the three stages of HHPV incubated by glybenclamide(Gly) and the combined application of Gly and U0126. At the same time, the values of rings' tension changes were recorded via the method of hypoxia hypercapnia conditions reactivity.</p><p><b>RESULTS</b>Under the normoxia condition, the values of the third pulmonary artery rings tension were relatively stable, but under the hypoxia hypercapnia condition, we observed a biphasic pulmonary artery contractile response compared with N group (P < 0.05, P < 0.01). When the third pulmonary artery rings incubated by Gly, it's phase II persistent vasoconstriction was enhanced compared with the H group (P < 0.05, P < 0.01), and the phase I vasoconstriction was also heightened. Moreover, under the hypoxia hypercapnia condition, U0126 could significantly relieve the phase II persistent vasoconstriction compared with HD group (P < 0.05, P < 0.01) induced by Gly, but the phase I acute vasoconstriction and the phase I vasodilation had no changes (P > 0.05).</p><p><b>CONCLUSION</b>Gly may mediate HHPV via activating ERK1/2 signal transduction pathway.</p>


Subject(s)
Animals , Glyburide , Pharmacology , Hypercapnia , Metabolism , Hypoxia , Metabolism , In Vitro Techniques , MAP Kinase Signaling System , Physiology , Male , Pulmonary Artery , Metabolism , Physiology , Rats , Rats, Sprague-Dawley , Vasoconstriction
9.
Article in Chinese | WPRIM | ID: wpr-325203

ABSTRACT

The aim of this study was to explore the effect of gambogic acid (GA) on MDS SKM-1 cell proliferation, apoptosis and their possible mechanism. Cell proliferation was determined by MTT method. The apoptosis percentage and cell cycle regulation of SKM-1 cells were analyzed by flow cytometry. Morphological features were observed by light microscopy. The mRNA expression of bcl-2 and bax were detected by RT-PCR. The results showed that GA could inhibit the proliferation of SKM-1 cells in a dose- and time-dependent manner (IC50 was 0.37 µg/ml at 48 h), increase the apoptotic percentage of SKM-1 cells, and arrest cell cycle at the G0/G1. The expression of bax mRNA was up-regulated while that of bcl-2 mRNA was down-regulated in SKM-1 cells treated with GA for 48 h. It is concluded that GA can induce apoptosis, which may be related to its effect of arresting cells at phase of G0/G1 and down-regulating bcl-2/bax ratio.


Subject(s)
Apoptosis , Cell Cycle Checkpoints , Cell Line, Tumor , Cell Proliferation , Humans , Myelodysplastic Syndromes , Metabolism , Pathology , Proto-Oncogene Proteins c-bcl-2 , Metabolism , Xanthones , Pharmacology , bcl-2-Associated X Protein , Metabolism
10.
Article in Chinese | WPRIM | ID: wpr-329940

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the role and significance of P38-MAPK in the pathological process of hypoxic hypercapnia pulmonary hypertension in rats, and the protection of panax notoginoside (PNS).</p><p><b>METHODS</b>(1) To set up rat pathological model of hypoxic hypercapnia pulmonary hypertension: seventy two male SD rats (200 280 g) were randomly divided into six groups (n = 12), which were normal group (N group), hypoxic hypercapnia for 3-day group (H3d), hypoxic hypercapnia for 1-week group(H1w), hypoxic hypercapnia for 2-week group (H2w), hypoxic hypercapnia for 4-week group (H4w) and PNS-injected group (Hp). The rats of PNS -injected group were injected PNS before being placed in the chamber (50 mg/(kg x d), ip), and other groups were injected normal sodium (2 ml/kg, ip). (2) The shapes of pulmonary artery were detected by HE staining. (3) Western blot was used to study the protein expression of p38-MAPK. The expression of p38-MAPK in lung tissue and pulmonary blood vessel was investigated by immunohistochemistry.</p><p><b>RESULTS</b>(1) The ratio of vessel wall area/total area (WA/ TA) in H1w, H2w, H4w and Hp group was higher than that of N group (P < 0.05), but that of H3d group did not change obviously (P > 0. 05 vs N group). The ratio of WA/TA in Hp group was obviously lower than that of H4w, group (P < 0.05). (2) The levels of P-p38 protein was markedly ascended in H3d group (0.225 +/- 0.071) compared with N group (0.012 +/- 0.006), and expression of P-p38 protein was significantly positive in H1w, H2w, H4w groups. (P < 0.05). (3) As P-p38 protein in pulmonary arterial tunica intima and tunica media, sterile expression in N group (0.099 +/- 0.015) and H3d group (0.107 +/- 0.013) contrasted to H4w group (0.124 +/- 0.025, P < 0.05), then tended to rise in H2w, H4w group (P < 0.05). (4) In pulmonary tissue, the levels of P-p38 protein in PNS-injected group were lower 53.02% (P < 0.05) than those in H4w group. In pulmonary arterial tunica intima and tunica media the levels of P-p38 protein in PNS-injected group were lower 87.33% (P < 0.05) than those in H4w group.</p><p><b>CONCLUSION</b>p38-MAPK as a signal transduction may play an important role in the development of hypoxia induced pulmonary hypertension. The effect of PNS on reducing pulmonary hypertension and improving pulmonary vascular wall remodeling may be related to its inhibiting expression of p38 MAPK.</p>


Subject(s)
Animals , Ginsenosides , Pharmacology , Therapeutic Uses , Hypertension, Pulmonary , Drug Therapy , Metabolism , Hypoxia , Metabolism , MAP Kinase Signaling System , Male , Panax notoginseng , Phytotherapy , Pulmonary Artery , Metabolism , Rats , Rats, Sprague-Dawley , p38 Mitogen-Activated Protein Kinases , Metabolism
11.
Article in Chinese | WPRIM | ID: wpr-329901

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of Notoginsenoside Rgl on p38 mitogen activated protein kinase (p38MAPK) expression in pulmonary artery smooth muscle cells (PASMCs) cultured in hypoxia hypercapnia.</p><p><b>METHODS</b>SD rat PASMCs was primary cultured, the cells of passage 2- 5 were divided into six groups: normoxic group (N group), hypoxia hypercapnia group (H group), dimethyl sulfoxide (DMSO) control group (HD group), Rg1 treated group (Rg low dose, Rg middle dose and Rg high dose group). Western blot was used to detect the expression of p-p38MAPK protein, and RT-PCR to determine the expression of p38MAPK mRNA.</p><p><b>RESULTS</b>Western blot and RT-PCR analysis indicated that the expression of p-p38MAPK protein and p-p38MAPK mRNA were significantly higher in HD group than those in N group (P < 0.01). Whereas, in Rg1 treated groups, the level of p-p38MAPK markedly decreased (P < 0.01) in dose-dependent manner.</p><p><b>CONCLUSION</b>Notoginsenoside Rg1 has protective effects on PASMCs under hypoxia hypercapnia condition, which may be related to inhibiting expression of p38MAPK.</p>


Subject(s)
Animals , Cell Hypoxia , Cells, Cultured , Ginsenosides , Pharmacology , Hypercapnia , Metabolism , Male , Muscle, Smooth, Vascular , Cell Biology , Myocytes, Smooth Muscle , Pulmonary Artery , Cell Biology , RNA, Messenger , Genetics , Rats , Rats, Sprague-Dawley , p38 Mitogen-Activated Protein Kinases , Metabolism
12.
Chinese Journal of Endemiology ; (6): 156-158, 2012.
Article in Chinese | WPRIM | ID: wpr-643264

ABSTRACT

ObjectiveTo investigate the effect of drinking brick tea with low-fluoride level on prevention of tea type fluorosis.MethodsHandahangacha,Hadayinggegacha,Dalainuoyi town,in Keshiketengqi Inner Mongolia endemic fluorosis area were selected as test points,and brick tea with fluoride [(204.5 ± 10.2),(308.2 ±15.4)mg/kg] was given for 12 months.Dental fluorosis,clinical skeletal fluorosis,and X-ray diagnosis of skeletalfluorosis [according to “Endemic Skeletal Fluorosis Diagnostic Criteria” (WS 192-2008)] of adults 20 to 70 years of age were examined and level of fluoride before and after the prevention trial,in brick tea,drinking water,milk tea and urine were tested (fluoride ion selective electrode method),and fluoride intake through tea was calculated.ResultsDetection rate of adult dental fluorosis in Handahangacha was 68.89% (62/90),clinical detection of skeletal fluorosis was 55.32% (52/94),and X-ray detection of skeletal fluorosis was 65.17% (58/89); adult dental fluorosis detection rate in Hadayinggegacha was 54.84%(51/93),clinical detection of skeletal fluorosis was 65.69%(67/102),and X-ray detection rate of skeletal fluorosis was 61.36% (54/88).Brick tea fluoride was (831.4 ±138.9),(864.3 ± 134.6)mg/kg before the prevention trial in Handahangacha and Hadayinggegacha,respectively,drinking water fluoride content was (0.27 ± 0.05),(0.54 ± 0.24)mg/L,fluoride content of milk tea was (216 ± 1.12),(2.82 ± 1.38)mg/L,adult urine fluoride content was (2.78 ± 1.57),(2.96 ± 1.80)mg/L,and fluoride intake through milk tea was (8.12 ± 5.84),(6.42 ± 5.04)mg/d,respectively; after the prevention trial the fluoride content of brick tea was (204.5 ± 10.2),(308.2 ± 15.4)mg/kg,fluoride content of drinking water (0.34 ± 0.11),(0.62 ± 0.30)mg/L,fluoride content of milk tea(0.97 ± 0.33),(1.83 ± 0.66)mg/L,fluoride content in urine(1.29 ± 0.55),( 1.47 ±0.62)mg/L,fluoride intake through milk tea (3.45 ± 2.05),(3.71 ± 2.07)mg/d,respectively; in Handahan and Hadayinggegacha after the prevention trial the fluoride in brick tea,milk tea,urine fluoride,and fluoride intake through milk tea was significantly lower than that before the trial (t =14.30,12.97 ;6.46,3.95;6.69,5.72;6.27,3.57,all P < 0.01 ).Fluoride intake in Handahangacha through milk tea was within the state heath standard limits( < 3.5mg/d).ConclusionDrinking low-fluoride brick tea can prevent drinking brick tea type fluorosis,the preventive effect is especially more reliable with low fluoride brick tea (204.5 ± 10.2)mg/kg.

13.
Chinese Journal of Surgery ; (12): 1565-1568, 2010.
Article in Chinese | WPRIM | ID: wpr-270916

ABSTRACT

<p><b>OBJECTIVE</b>To clarify the significance of micrometastases in pelvic lymph nodes in patients with neoadjuvant hormonal therapy (NHT) before radical prostatectomy (RP).</p><p><b>METHODS</b>Twenty-one patients with clinically localized prostate cancer who received NHT between August 2007 and March 2010 were observed. The patients were clarified into four groups: pathological examination was positive (group A), real-time PCR examination targeting prostate specific antigen (PSA) mRNA and prostate specific membrane antigen (PSMA) mRNA were positive (group B), pathological examination and real-time PCR examination targeting PSA mRNA and PSMA mRNA were both negative (group C), and the control group (group D). After a standard bipedal lymphangiography the films were reviewed carefully by an experienced radiologist. If positive lymph nodes were seen or suspected, a thin-walled 22 gauge needle were directed transabdominally under fluoroscopic control into the area of question and an aspirate was obtained. The expression of PSA and PSMA in aspirate were assessed by a fully quantitative real-time PCR. The specimens were regarded in which either PSA mRNA or PSMA mRNA were positive as showing the "presence of micrometastasis". Lymph node specimens were also stained immunohistochemically with an antibody PSA after RP.</p><p><b>RESULTS</b>Pathological examination detected lymph node metastases from 3 cases, and real-time PCR further identified lymph node micrometastases from 14 cases with no pathological evidence of nodal involvement. The expression level of PSA mRNA and PSMA mRNA were statistically significant in patients with histological confirmed lymph node metastases and micrometastases detected by real-time PCR despite the lack of histological evidence, and the expression level of PSA mRNA and PSMA mRNA in aspirate were higher than the lymph node between the group A and group B.</p><p><b>CONCLUSIONS</b>Although residual foci of atrophic prostate cancer cells in resected lymph nodes after NHT can be difficult to diagnose by pathological examination, the present results show the usefulness of quantitative real-time PCR targeting PSA and PSMA mRNA for detected micrometastatic tumour foci in pelvic lymph nodes from fine needle aspiration biopsy of lymph nodes before RP.</p>


Subject(s)
Aged , Humans , Lymph Nodes , Pathology , Lymphatic Metastasis , Pathology , Male , Middle Aged , Pelvis , Pathology , Polymerase Chain Reaction , Methods , Preoperative Care , Prognosis , Prostate-Specific Antigen , Genetics , Metabolism , Prostatic Neoplasms , Pathology , General Surgery , RNA, Messenger , Genetics
14.
Chinese Journal of Cancer ; (12): 451-455, 2010.
Article in English | WPRIM | ID: wpr-292562

ABSTRACT

As an essential metal for sustaining life, iron is involved in a number of metabolic processes, including DNA synthesis, electron transport, oxygen delivery, and so on. Iron metabolism involves the absorption, transport, and use of iron and is strictly regulated. Numerous studies have found a positive correlation between iron storage and the risk of tumors, such as colorectal carcinoma, hepatic cancer, renal carcinoma, lung cancer, and gastric cancer. In tumor cells, iron metabolism changes by several mechanisms, such as regulating the growth of tumor cells by transferrin, accelerating the uptake of iron by the overexpressions of transferrin receptors 1 and 2 (TfR1 and TfR2), synthesizing or secreting ferritin by some malignant tumor cells, and upregulating the level of hepcidin in patients with cancer. Some advances on diagnosis and treatment based on iron metabolism have been achieved, such as increasing the transfection and target efficiency of transferrin-polyethylenimine (PEI), inducing cell apoptosis by beta-guttiferin through interacting with TfR1.


Subject(s)
Animals , Antibiotics, Antineoplastic , Pharmacology , Antigens, CD , Genetics , Metabolism , Antimicrobial Cationic Peptides , Genetics , Apoptosis , Cell Proliferation , Doxorubicin , Pharmacology , Ferritins , Metabolism , Physiology , Hepcidins , Humans , Interleukin-18 , Pharmacology , Iron , Metabolism , Physiology , Neoplasms , Metabolism , Pathology , RNA, Messenger , Metabolism , Receptors, Transferrin , Genetics , Metabolism , Transferrin , Metabolism , Physiology , Tumor Suppressor Protein p53 , Pharmacology
15.
Article in Chinese | WPRIM | ID: wpr-287885

ABSTRACT

In this review, we described the taxonomic study of the Fritillaria medicinal plant in the recent years. The taxonomic study of the Fritillaria medicinal plant was carried out from three main aspects: the traditional morphological character, the characteristic constituents of the plant and genotyping and species identification of Fritillaria by DNA chips. By comparison, we concluded that the DNA chip technology can provide a rapid, high throughput for genotyping and quality assurance of the plant species verification. It is the most prosperous method of species identification of the plant.


Subject(s)
Cevanes , Flowers , Chemistry , Genetics , Fritillaria , Classification , Genetics , Genotype , Oligonucleotide Array Sequence Analysis , Plant Leaves , Chemistry , Genetics , Plants, Medicinal , Classification , Genetics
16.
Chinese Medical Journal ; (24): 1783-1787, 2007.
Article in English | WPRIM | ID: wpr-255505

ABSTRACT

<p><b>BACKGROUND</b>Severe burn-blast combined injury is a great challenge to medical teams for its high mortality. The aim of this study was to elucidate the clinical characteristics of the injury and to present our clinical experiences on the treatment of such cases.</p><p><b>METHODS</b>Five patients with severe burn-blast combined injuries were admitted to our hospital 77 hours post-injury on June 7, 2005. The burn extent ranged from 80% to 97% (89.6% +/- 7.2%) of TBSA (full-thickness burns 75% - 92% (83.4% +/- 7.3%)). All the patients were diagnosed as having blast injury and moderate or severe inhalation injury. Functions of the heart, liver, kidney, lung, pancreas and coagulation were observed. Autopsy samples of the heart, liver, and lungs were taken from the deceased. Comprehensive measures were taken during the treatment, including protection of organ dys function, use of antibiotics, early anticoagulant treatment, early closure of burn wounds, etc. All the data were analyzed statistically with t test.</p><p><b>RESULTS</b>One patient died of septic shock 23 hours after admission (four days after injury), the others survived. Dysfunction of the heart, liver, lungs, pancreas, and coagulation were found in all the patients on admission, and the functions were ameliorated after appropriate treatments.</p><p><b>CONCLUSIONS</b>Burn-blast combined injury may cause multiple organ dysfunctions, especially coagulopathy. Proper judgment of patients' condition, energetic anticoagulant treatment, early closure of burn wounds, rational use of antibiotics, nutritional support, intensive insulin treatment, timely and effective support and protection of organ function are the most important contributory factors in successful treatment of burn-blast combined injuries.</p>


Subject(s)
Adult , Anti-Bacterial Agents , Therapeutic Uses , Blast Injuries , Therapeutics , Burns , Therapeutics , Humans , Male , Nutrition Therapy , Psychotherapy , Respiration
17.
Article in Chinese | WPRIM | ID: wpr-245990

ABSTRACT

<p><b>OBJECTIVE</b>To provide a quick and simple method to identify different Chinese medicine material of Fritillaria.</p><p><b>METHOD</b>The thermograms and differential thermograms of nine Fritillaria powders were obtained by thermal analyzer.</p><p><b>RESULT</b>By analyzing the thermograms of nine Fritillaria powders, we concluded that the thermal stability of nine Fritillaria powders was much different each other due to the different geography origin. The thermal stability of F. hupehensis was highest among nine Fritillaria, while F. ussurensis was the lowest. The different Fritillaria showed their own DTA spectra respectively.</p><p><b>CONCLUSION</b>According to the differences in the thermal properties of nine Fritillaria powders, the origins of Fritillaria could be easily identfied.</p>


Subject(s)
Calorimetry, Differential Scanning , Methods , China , Climate , Ecosystem , Fritillaria , Chemistry , Classification , Hot Temperature , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Classification , Powders
18.
Article in Chinese | WPRIM | ID: wpr-358085

ABSTRACT

<p><b>OBJECTIVE</b>To provide a simple method to identify different of herbal drugs of Fritillaria.</p><p><b>METHOD</b>The X-ray diffraction spectra of five Fritillaria powders were obtained by X-ray diffractometry (XRD).</p><p><b>RESULT</b>By analyzing the diffraction peaks of starch over the 2theta diffraction angle in the range of 10 degrees-30 degrees, we concluded that the crystal type of starch in F. thunbergii, F. ussurensis, F. pallidifloca and F. hupehensis was B-type. However, the crystal type of starch in F. cirrhosa was CB-type. The degree of crystallinity of starch was 42.1%, 43.6%, 37.7%, 35.9% and 30.8%, corresponding to F. thunbergii, F. ussurensis, F. pallidiflcoca,F. cirrhosa and F. hupehensis.</p><p><b>CONCLUSION</b>Based on the differences of crystallinity of starch and X-ray diffraction spectra of Fritillaria powders, different species of Fritillaria can be identified.</p>


Subject(s)
Crystallization , Fritillaria , Chemistry , Classification , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Classification , Species Specificity , Starch , X-Ray Diffraction
19.
Acta Pharmaceutica Sinica ; (12): 791-794, 2003.
Article in Chinese | WPRIM | ID: wpr-266601

ABSTRACT

<p><b>AIM</b>To compare lipophilicity measuring scale stemmed from immobilized artificial membrane chromatography and n-octanol/buffer systems.</p><p><b>METHODS</b>A test set consisted of 27 structurally diverse compounds. The lipophilicity of these were evaluated by both immobilized artificial membrane chromatography (IAMC) and n-octanol/buffer systems, which were expressed as lg kIAM and lg DO/W,7.4, respectively.</p><p><b>RESULTS</b>With regard to each individual group, good correlation coefficient (r2) over 0.81 was obtained (0.82 for acid; 0.88 for neutral, 0.81 for base and 0.92 for ampholyte, respectively). However, a smaller r2 (0.62) was acquired for all compounds studied than that of each individual group.</p><p><b>CONCLUSION</b>IAMC and n-octanol/buffer systems were shown to be different in lipophilicity.</p>


Subject(s)
Buffers , Chromatography, High Pressure Liquid , Methods , Hydrophobic and Hydrophilic Interactions , Lipids , Chemistry , Membranes, Artificial , Octanols , Chemistry , Pharmaceutical Preparations , Chemistry , Phospholipids , Chemistry
20.
Acta Pharmaceutica Sinica ; (12): 702-706, 2003.
Article in Chinese | WPRIM | ID: wpr-266585

ABSTRACT

<p><b>AIM</b>To investigate the interaction between drugs and ordered phospholipid membrane using immobilized artificial membrane chromatography (IAMC).</p><p><b>METHODS</b>IAMC was used to determine the interaction drugs with phospholipid membrane, expressed as membrane affinity (lg kIAM). An n-octanol/buffer system was also employed as the reference hydrophobicity (lg Do/w,7.4).</p><p><b>RESULTS</b>Within the range of used acetonitrile percentages (phi) 0-30% in mobile phase, retention index (lg kIAM) showed excellent correlation with phi. Intercepts of fitted straight lines between lg kIAM and phi were comparable but slopes were much different for the three organic modifiers (acetonitrile, ethanol and methanol). Effects by adding CH2 substituent on lipophilicity difference (delta lg kIAM and delta lg Do/w,7.4) were similar for p-hydroxyl benzoic methyl ester to butyl ester, whereas different for p-hydroxylbenzoic acid to methyl ester.</p><p><b>CONCLUSION</b>IAMC system is a convenient, efficient and rapid tool for determining membrane interaction.</p>


Subject(s)
Membranes, Artificial , Octanols , Chemistry , Pharmaceutical Preparations , Chemistry , Phospholipids , Chemistry
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