Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 72
Filter
1.
Chinese Journal of Pediatrics ; (12): 910-916, 2023.
Article in Chinese | WPRIM | ID: wpr-1013196

ABSTRACT

Objective: To investigate the poisonous substances and geographical distribution of poisoning in children in China. Methods: A cross-sectional study. The clinical data of 8 385 hospitalized children from January 2016 to December 2020 were extracted from the FUTang Updating Medical Records database. These children aged 0 to 18 years and were admitted due to poisoning. They were grouped according to age (newborns and infants, toddlers, preschoolers, school-age children, adolescents), place of residence (Northeast China, North China, Central China, East China, South China, Southwest China, Northwest China), and mode of discharge (discharge under medical advice, transfer to another hospital under medical advice, discharge without medical advice, death, other). The poisonous substance and causes of poisoning in different groups were analyzed. Results: Among these 8 385 children, 4 734 (56.5%) were male and 3 651 (43.5%) female, with a male-to-female ratio of 1.3∶1. The age was 3 (2, 7) years. The prevalence of poisoning was 51.8% (4 343/8 385) in toddlers, 16.5% (1 380/8 385) in adolescents, 14.8% (1 242/8 385) in preschoolers, 14.4% (1 206/8 385) in school-age children, and 2.5% (214/8 385) in newborns and infants. Drug poisoning accounted for 43.5% (3 649/8 385) and pesticide accounted for 26.8% (2 249/8 385). Drug poisoning was more common in adolescents (684/1 380, 49.6%) and toddlers (2 041/4 343, 47.0%); non-drug poisoning was more common in school-age children (891/1 206, 73.9%), of which carbon monoxide was mainly in newborns and infants (41/214, 19.2%) and food poisoning in children of school age (241/1 206, 20.0%). Regarding regional characteristics, drug poisoning was more frequent in South China (188/246, 64.2%) and non-drug poisoning was more frequent in Southwest China (815/1 123, 72.5%). For drugs, anti-epileptic drugs, sedative-hypnotic drugs and anti-Parkinson's disease drugs had a higher proportion of poisoning in North China (138/1 034, 13.0%) than that in other regions. For non-drug poisoning, pesticides (375/1 123, 33.3%), food poisoning (209/1 123, 18.6%) and contact with poisonous animals (86/1 123, 7.7%) were more common in Southwest China than in other regions; carbon monoxide poisoning was more common in North China (81/1 034, 7.6%) and Northwest China (65/1 064, 6.3%). In Central China, poisoning happened more in toddlers (792/1 295, 61.2%) and less in adolescents (115/1 295, 8.8%) than in other regions. Regarding different age groups, poisoning in adolescent happened more in Northeast China (121/457, 26.5%), North China (240/1 034, 23.2%), and Northwest China (245/1 064, 23.0%). The rate of discharge under medical advice, discharge without medical advice, and mortality rate within the 5 years were 77.0% (6 458/8 385), 20.8% (1 743/8 385), 0.5% (40/8 385), respectively. Conclusions: Poisoning is more common in male and toddlers. Poisonous substances show a regional characteristic and vary in different age groups, with drugs and insecticides as the most common substances.


Subject(s)
Infant , Adolescent , Animals , Child , Male , Humans , Infant, Newborn , Female , Child, Hospitalized , Cross-Sectional Studies , Carbon Monoxide Poisoning/epidemiology , Pesticides , Foodborne Diseases , Hospitals , Drug-Related Side Effects and Adverse Reactions , China/epidemiology
2.
Journal of Sun Yat-sen University(Medical Sciences) ; (6): 335-341, 2023.
Article in Chinese | WPRIM | ID: wpr-965850

ABSTRACT

ObjectiveTo discuss the correlation between young patients’ adverse transfusion reaction and pre-transfusion C reactive protein (CRP) level and EO% (percentage of eosinophils) . MethodsThe observation group was chosen from among sixty-six young patients who experienced transfusion-related adverse events between January 2019 and December 2020. For each patient chosen to be included in the observation group, another patient from the same department, with the same disease and gender, who had been hospitalized in the same month and received the same type of blood product transfusion, but had not experienced any transfusion-related adverse effects, was chosen to be in the control group. We examined and compared their ages, transfusion experiences, allergy backgrounds, EO%, and CRP levels in peripheral blood prior to transfusion. A receiver operating characteristic (ROC) curve was used to examine the diagnostic value of EO% and CRP for transfusion-related adverse events. Simultaneously, a logistic analysis was performed on the risk factors for transfusion-related adverse events. ResultsPre-transfusion CRP was higher in patients with FNHTR in the observation group than it was in patients in the control group; pre-transfusion CRP was also higher in patients with ATR in the observation group than it was in patients in the control group. There were also statistically significant differences between these variables in the percentage of patients with transfusion history and pre-transfusion EO% ( P<0.05). For the transfusion of different blood types, there was statistical significance in the occurrence of ATR and FNHTR ( P<0.05). For the diagnosis of FNHTR, the CRP area under the ROC curve was 0.889, and the best cut-off value was 18.05 mg/L. For the diagnosis of ATR, the area under the ROC curve was 0.749, and the best cut-off values were 17.60 mg/L. ConclusionPre-transfusion C-reactive protein level is an independent risk factor for FNHTR and ATR in young patients; the predictive value of EO% for adverse blood transfusion reactions is insufficient.

3.
Chinese Medical Journal ; (24): 2976-2984, 2021.
Article in English | WPRIM | ID: wpr-921232

ABSTRACT

BACKGROUND@#Prospective analyses have yet to identify a consistent relationship between sleep duration and the incidence of gastrointestinal (GI) cancers. The effect of changes in sleep duration on GI cancer incidence has scarcely been studied. Therefore, we aimed to examine the association between baseline sleep duration and annual changes in sleep duration and GI cancer risk in a large population-based cohort study.@*METHODS@#A total of 123,495 participants with baseline information and 83,511 participants with annual changes in sleep duration information were prospectively observed from 2006 to 2015 for cancer incidence. Cox proportional-hazards models were used to calculate hazard ratios (HRs) and their confidence intervals (CIs) for GI cancers according to sleep duration and annual changes in sleep duration.@*RESULTS@#In baseline sleep duration analyses, short sleep duration (≤5 h) was significantly associated with a lower risk of GI cancer in females (HR: 0.31, 95% CI: 0.10-0.90), and a linear relationship between baseline sleep duration and GI cancer was observed (P = 0.010), especially in males and in the >50-year-old group. In the annual changes in sleep duration analyses, with stable category (0 to -15 min/year) as the control group, decreased sleep duration (≤-15 min/year) was significantly associated with the development of GI cancer (HR: 1.29; 95% CI: 1.04-1.61), especially in the >50-year-old group (HR: 1.32; 95% CI: 1.01-1.71), and increased sleep duration (>0 min/year) was significantly associated with GI cancer in females (HR: 2.89; 95% CI: 1.14-7.30).@*CONCLUSIONS@#Both sleep duration and annual changes in sleep duration were associated with the incidence of GI cancer.


Subject(s)
Female , Humans , Male , Middle Aged , Cohort Studies , Gastrointestinal Neoplasms/etiology , Incidence , Proportional Hazards Models , Prospective Studies , Risk Factors , Sleep
4.
Journal of Clinical Hepatology ; (12): 2819-2823, 2021.
Article in Chinese | WPRIM | ID: wpr-906869

ABSTRACT

Objective To investigate the value of transient elastography (FibroScan) and platelet count-to-spleen thickness (PC/ST) ratio in predicting the degree of esophageal and gastric varices (EGV) in liver cirrhosis. Methods A total of 210 patients with liver cirrhosis who underwent FibroScan, color Doppler ultrasound, electronic gastroscopy, and blood biochemical examination within three days after admission to The Fifth Affiliated Hospital of Zhengzhou University from January 2017 to September 2020 were enrolled, and according to the "gold standard" of gastroscopy, the patients were divided into none, mild, moderate, and severe EGV groups. A one-way analysis of variance or the Kruskal-Wallis H test were used for comparison of continuous data between multiple groups. Receiver operating characteristic (ROC) curves were plotted for liver stiffness measurement (LSM), PC/ST ratio, and LSM+PC/ST, and the diagnostic performance of these ROC curves was compared using the Delong method. Results There were significant differences between the none, mild, moderate, and severe EGV groups in LSM ( F =32.00, P < 0.01) and PC/ST ratio ( H =49.58, P < 0.01). For the mild EGV group, LSM, PC/ST ratio, and LSM+PC/ST had an area under the ROC curve (AUC) of 0.762, 0.656, and 0.770, respectively, with a positive predictive value of 75.4%, 60.2%, and 82.5%, respectively, and a negative predictive value of 75.0%, 75.8%, and 65.4%, respectively. For the moderate EGV group, LSM, PC/ST ratio, and LSM+PC/ST had an AUC of 0.841, 0.796, and 0.896, respectively, with a positive predictive value of 86.1%, 68.0%, and 74.1%, respectively, and a negative predictive value of 79.7%, 80.5%, and 90.2%, respectively. For the severe EGV group, LSM, PC/ST ratio, and LSM+PC/ST had an AUC of 0.834, 0.830, and 0.903, respectively, with a positive predictive value of 80.5%, 71.4%, and 79.5%, respectively, and a negative predictive value of 82.5%, 83.6%, and 85.0%, respectively. PC/ST ratio and LSM+PC/ST had significantly different AUC in predicting mild, moderate, and severe EGV ( Z =2.66, P =0.007; Z =2.71, P =0.007; Z =2.37, P =0.018). Conclusion LSM, PC/ST ratio, and LSM+PC/ST have a good predictive value for moderate-to-severe EGV in liver cirrhosis.

5.
Chinese Journal of Otorhinolaryngology Head and Neck Surgery ; (12): 586-589, 2021.
Article in Chinese | WPRIM | ID: wpr-942484

ABSTRACT

Objective: To investigate the middle ear function of the patients with cleft palate pre and post palatoplasty. Methods: 76 patients with cleft palate were investigated by clinical history and audiology examinations including electric otoscopy,tympanometry and click-ABR threshold. Results: The risk for middle ear function decreased with advancing age in the first 5 years. It was noticed that the otologic outcomes was related to the CP type. During long time follow-up, the frequency with the middle ear function disorder was always high within the CP patients but the proportion of the patients received tympanostomy tubes was low relatively. The prevalence of middle ear dysfunction did not differ with the time of cleft palate repair. Conclusion: The patients with cleft palate have middle ear function dysfunction in a long period of time,therefore a standard long-time follow-up system is necessary.


Subject(s)
Child, Preschool , Humans , Acoustic Impedance Tests , Cleft Palate/surgery , Ear, Middle , Middle Ear Ventilation , Otitis Media with Effusion/surgery
6.
Chinese Journal of Infectious Diseases ; (12): 97-102, 2021.
Article in Chinese | WPRIM | ID: wpr-884188

ABSTRACT

Objective:To explore the epidemiological characteristics and the antibiotic resistance of Streptococcus pneumoniae isolates, and to provide the evidence for the rational use of antimicrobial agents to treat Streptococcus pneumoniae infection. Methods:The positive microbiological laboratory identification and antimicrobial susceptibility testing of Streptococcus pneumoniae from sputum of children with respiratory infections during January 2010 to December 2017 in Children′s Hospital of Soochow University were retrospectively analyzed. The positive rates of Streptococcus pneumoniae of different genders, ages, years and seasons were compared. The annual detection rates and trends of drug resistance of Streptococcus pneumoniae to penicillin, amoxicillin and cefotaxime were analyzed by Mann-Kendall trend test. The seasonal decomposition of time series was conducted to assess the association between Streptococcus pneumoniae detection rate and season. Enumeration data was compared using χ2 test. Results:Of the 88 480 sputum specimens, the total positive rate of Streptococcus pneumoniae was 10.3%(9 081/88 480). The detection rates of Streptococcus pneumoniae in children aged 0 to <0.5 years old, 0.5 to <2 years old, 2 to <3 years old, 3 to <5 years old, and 5 to <15 years old were 4.2%(1 407/33 224), 13.1%(3 191/24 390), 14.9%(2 417/16 252), 17.9%(1 474/8 246) and 9.3%(592/6 368), respectively. The difference was statistically significant ( χ2=2 421.6, P<0.01). The detection rates were 8.1%(1 321/16 306) from January to March, 10.9%(2 194/20 207) from April to June, 8.5%(2 141/25 058) from July to September, and 12.7%(3 425/26 909) from October to December. The discrepancy of positive rates in different seasons showed statistical significance ( χ2=311.5, P<0.01). During 2010 to 2017, significant decreases in antibiotic resistant rates of Streptococcus pneumoniae to penicillin, amoxicillin and cefotaxime were detected (tau=-0.93, -0.93 and -0.71, respectively, all P<0.05). Conclusions:The detection rate of Streptococcus pneumoniae in sputum of children with respiratory infections may present seasonal pattern and vary between different ages of children. The resistance to β-lactam antibiotics has declined.

7.
Biomedical and Environmental Sciences ; (12): 348-355, 2021.
Article in English | WPRIM | ID: wpr-878370

ABSTRACT

Objective@#To examine increases in average height among Chinese children and adolescents.@*Methods@#The data were obtained from the China Health and Nutrition Survey conducted during the period 1989-2015. A stratified multistage cluster sampling method was utilized to select participants aged 2-22 years in each province. Linear regression was used to examine the effects of age, birth cohort, and survey period on height.@*Results@#A total of 15,227 males and 13,737 females were included in the final analysis. Age (A) showed a continuous effect on height. The average heights of the investigated groups increased continuously during the investigation period. By 2015, the average height of the overall group increased by 7.87 cm compared to the average height during the 1989 survey. Moreover, birth year (cohort, C) also had a stable effect on height. Using the height of individuals born in or before 1975 as a reference, the average height of each birth cohort increased in comparison to the previous birth cohort.@*Conclusions@#The height of Chinese children and adolescents was affected by age, period, and cohort effects, and this effect is governed by certain rules. The age-period-cohort model can be used to analyze the trends of children's and adolescent's heights. The findings provide a scientific basis for the formulation of children's and adolescents' growth and development policies in China.


Subject(s)
Adolescent , Child , Child, Preschool , Female , Humans , Male , Young Adult , Adolescent Development , Adolescent Health , Age Factors , Body Height , Child Development , Child Health , China , Health Status Indicators , Health Surveys , Linear Models , Sex Factors
8.
Chinese Journal of Hepatobiliary Surgery ; (12): 274-280, 2020.
Article in Chinese | WPRIM | ID: wpr-868813

ABSTRACT

Objective:To investigate the expression of IKBKE and NF-κB in pancreatic cancer, and to explore the effect of IKBKE on pancreatic cancer proliferation and migration.Methods:Immunohistochemistry staining was used to study the expression of IKBKE and NF-κB in tissues of 61 pancreatic cancer patients admitted to the Second Hospital of Tianjin Medical University from January 2012 to January 2017 and 13 normal pancreatic tissues. The correlations between those expression to clinicopathological features were analyzed. Lentivirus mediated RNAi was transfected into pancreatic cancer cells to block IKBKE. Western blot was performed to test the silencing effeciency; CCK-8 and plate clone and scratch assays were used to investigate the proliferation and migration of pancreatic cancer.Results:Immunohistochemical staining showed that 60 (98.4%) of IKBKE staining were weakly positive, positive, and strongly positive in pancreatic cancer tissues, which were significantly higher than normal pancreatic tissues(76.9% cases were weakly positive and the rest were negative), and the differences were statistically significant ( P<0.05). All cases of NF-κB exhibited weakly positive expression and above in pancreatic cancer tissues, which was markedly higher than normal tissues (30.8% cases were weak positive and the rest were negative staining), statistically significant ( P<0.05). Survival analysis showed that patients with high level of IKBKE showed a shorter overall survival ( P<0.05). CCK-8, plate cloning and scratch assays showed that the proliferation and migration of were significantly decreased in IKBKE knocking down group ( P<0.05). Conclusions:IKBKE and NF-κB are highly expressed in pancreatic cancer, and IKBKE is correlated with NF-κB in pancreatic cancer. Blocking of IKBKE could distinctly inhibit the proliferation and migration of pancreatic cancer.

9.
Chinese Journal of Disease Control & Prevention ; (12): 31-36, 2020.
Article in Chinese | WPRIM | ID: wpr-793313

ABSTRACT

Objective To evaluate clinical factors associated with laboratory-confirmed influenza infection and probe into the effects of different influenza-like illness(ILI) definitions on influenza surveillance in hospitalized children. Methods The influenza surveillance on the hospitalized children with acute respiratory infection was carried out in Soochow university affiliated children’s hospital from October 2017 to May 2018. The definition of influenza-like illness(ILI), which proposed by world health organization (WHO), the European center for disease prevention and control (ECDC) and ministry of public health of China, was analyzed to determine the area under receiver operating characteristic curve (ROC), sensibility, specificity, and the positive and negative predictive values of the ILI definition using the laboratory evidence of influenza virus infection as golden criteria. Logistic regression model was employed to explore the risk factors of the laboratory confirmed influenza infection among the hospitalized children. Results Of the enrolled 1 459 hospitalized children, 204(14.0%) were lab-confirmed influenza cases. The ECDC definition had the highest sensitivity (91.7%, 95% CI: 87.9%-95.5%) but the lowest specificity (44.6%, 95% CI: 41.9%-47.4%). The WHO definition had the lowest specificity (70.6%, 95% CI: 64.3%-76.8%). China’s definition was the most discriminant definition with relatively high sensitivity (91.2%, 95% CI: 87.3%-95.1%) and specificity (51.5%, 95% CI: 48.8%-54.3%), and its positive area under curve value (71.2%, 95% CI: 67.9%-74.5%) was the highest. Multivariate analysis model showed that the detection rate of influenza virus in children with fever (≥38 ℃) was higher than that in children without fever (OR=7.03, 95% CI:3.89-12.70). Conclusions It is suggested to adopt China’s ILI definition to get better output during influenza surveillance among hospitalized children.

10.
Experimental Neurobiology ; : 138-149, 2020.
Article | WPRIM | ID: wpr-832439

ABSTRACT

Neuropsychiatric disorders are the leading cause of mental and intellectual disabilities worldwide. Current therapies against neuropsychiatric disorders are very limited, and very little is known about the onset and development of these diseases, and their most effective treatments. MIR137 has been previously identified as a risk gene for the etiology of schizophrenia, bipolar disorder, and autism spectrum disorder. Here we generated a forebrain-specific MIR137 knockout mouse model, and provided evidence that loss of miR-137 resulted in impaired homeostasis of potassium in mouse hippocampal neurons. KCC2, a potassium-chloride co-transporter, was a direct downstream target of miR-137. The KCC2 specific antagonist VU0240551 could balance the current of potassium in miR-137 knockout neurons, and knockdown of KCC2 could ameliorate anxiety-like behavior in MIR137 cKO mice. These data suggest that KCC2 antagonists or knockdown might be beneficial to neuropsychiatric disorders due to the deficiency of miR-137.

11.
Chinese Journal of School Health ; (12): 1052-1054, 2020.
Article in Chinese | WPRIM | ID: wpr-823177

ABSTRACT

Objective@#To study the relationship between nutrition status and physical fitness in 8-10 year-old children in 3 cities, and to provide a scientific reference for improving physical condition of chidren of pre-school age.@*Methods@#To investigate the weight, height and physical fitness (standing long jump, endurance running, rope skipping in one minute and sit-ups in one minute) of 1 384 children in grade four from 24 primary schools in Beijing, Changzhi, Urumchi in 2018. Evaluated overweight and obesity by using the standard of “Screening for Overweight and Obesity among School-age Children”. Then used mixed linear model to compare the physical fitness of students with different nutritional status and to analyze the relationship between BMI and physical fitness.@*Results@#The prevalence of overweight and obesity were 17.41% and 23.48%, respectively. And the prevalence in boys was both more than that in girls(χ2=9.84,47.68,P<0.01). The increase in BMI of children from the same age and the same gender was related with the decrease in physical fitness by correlation analysis(P<0.05). In comparison method, the performance of the students of normal weight was better than obese students, but the male and female students had got the same results in their physical fitness test(P<0.05).@*Conclusion@#There is a negative correlation between nutrition status and physical fitness and the physical fitness in normal weight and obese children are better than the overweight children or obese children.

12.
Journal of Sun Yat-sen University(Medical Sciences) ; (6): 233-242, 2020.
Article in Chinese | WPRIM | ID: wpr-817696

ABSTRACT

@#【Objective】 To explore the effects and the possible mechanism of RNA targeting membrane-bound prostaglandin E2 synthase l(mPGES- 1)on proliferation,apoptosis and drug resistance of leukemia cell line K562/A.【Methods】RNA interference was used to inhibit the expression of mPGES-1 of K562/A cells. Four groups were set up as follows:untreated group(K562/A),negative control group after interference(K562/A-NC),group after interference(K562/ A-KD),and group after interference with exogenous PGE2(K562/A-KD+PGE2).Cell viability was assessed by CCK-8 assay. Cell apoptosis was analyzed by flow cytometry. Concentration of PGE2 was detected by ELISA. Proteins expression was detected by western blot.【Results】The expression of mPGES- 1 in K562/A cells was significantly down- regulated and the synthesis of PGE2 decreased(P < 0.000 1)after RNA interference. After RNA interference,the proliferation of K562/A cells was inhibited and apoptosis increased,and the sensitivity to chemotherapy drugs was enhanced(P < 0.05). Meanwhile,the expression of β-catenin and MDR1 was decreased(P < 0.01). Exogenous PGE2 could reverse the effect of RNA interference on proliferation ,apoptosis and drug sensitivity in K562/A cells(P < 0.05),and up-regulate the expression of β-catenin and MDR1(P < 0.01). XAV939,an inhibitor of β-catenin,could down-regulate the expression of β- catenin and MDR1 in an dose- dependent pattern in K562/A cells(P < 0.05).【Conclusions】RNA interference of mPGES- 1 could inhibit proliferation,induce apoptosis and reverse drug resistance in K562/A cells. The mechanism was related to reducing the synthesis of PGE2 and thus down- regulating the expression of β- catenin and MDR1. Wnt/β- catenin signal pathway may participate in the regulation of MDR1 by mPGES-1/PGE2.

13.
Chinese Journal of Interventional Imaging and Therapy ; (12): 107-111, 2019.
Article in Chinese | WPRIM | ID: wpr-862176

ABSTRACT

Objective: To investigate the impact of diagnostic ultrasound (DUS) with different mechanical index (MI) combined with microbubbles on tumor blood flow and chemotherapeutic drug concentration in nude mice bearing human pancreatic cancer. Methods: Pancreatic tumor models were established through injecting tumor cell suspension into bilateral hind limbs in 33 nude mice,which were divided into three groups (group A, B and C) randomly. Contrast-enhanced ultrasound (CEUS), chemotherapeutic agents injection and exposure/sham exposure with DUS were performed on all models in the three groups. For each model, one of the hind limb was chosen as therapeutic side (exposure), and the other hind limb as control side (sham exposure). CEUS was performed before and after ultrasonic exposure/sham to obtain the time-intensity curve (TIC), peak intensity (PI) and AUC. Doxorubicin was injected through the tail vein before ultrasonic exposure/sham exposure. And microbubbles were continuously injected during ultrasonic exposure/sham exposure. MI of therapeutic side for ultrasonic exposure in group A, B and C was 0.3, 0.7 and 1.1, respectively. After ultrasonic exposure/sham exposure, Doxorubicin concentration in vitro tumor tissue was detected, and then histological analysis was done. In addition, the range of peak negative pressure correspond with different MI were detected before ultrasonic exposure/sham exposure. Results: Concentration of Doxorubicin of therapeutic side in group A was obviously higher than that of control side ([1.45±0.53]μg/g vs [1.07±0.46]μg/g; t=-5.163, P=0.001). In group B and C, there was no statistically significant difference of Doxorubicin concentration between therapeutic side and control side in the drug concentration (Z=-0.297, -0.357, P=0.766, 0.721). Quantitative analysis of CEUS showed that both PI and AUC increased obviously after ultrasonic exposure/sham exposure compared with those before exposure/sham exposure (all P<0.05), except for PI of control side in group B and AUC of control side in group B and C. Neither hemorrhage nor swelling was observed in tumor tissue of both sides in any group. The range of peak negative pressure correspond with MI of 0.3 (group A), 0.7 (group B) and 1.1 (group C) was 0.81-0.83 MPa, 0.96-1.32 MPa and 2.29-2.53 MPa, respectively. Conclusion: DUS (MI=0.3) combined with microbubbles can enhance blood perfusion and improve the chemotherapeutic drug concentration of tumor tissues in nude mice bearing human pancreatic cancer.

14.
Chinese Journal of Rehabilitation Theory and Practice ; (12): 196-200, 2019.
Article in Chinese | WPRIM | ID: wpr-905098

ABSTRACT

Axonal damage leads to permanent defects in the adult mammals central nervous system. As an important axonal growth inhibitor, Nogo-A and its receptors involve in the regulation of synaptic plasticity in mature neurons of the central nervous system, and play a role in the related neurological diseases, such as spinal cord injury, multiple sclerosis, Alzheimer's disease and posttraumatic stress disorder.

15.
Journal of Sun Yat-sen University(Medical Sciences) ; (6): 37-45, 2019.
Article in Chinese | WPRIM | ID: wpr-817657

ABSTRACT

@#【Objective】To explore the effects and the possible mechanism of KPT- 8602,a novel selective inhibitor of nuclear export protein (XPO1),on proliferation,cell cycle and apoptosis in human histiocytic lymphoma cell line U937 cells.【Methods】U937 cells were treated with different concentrations of KPT- 8602. Cell viability was assessed by CCK-8 assay. The cell cycle distribution and the apoptosis rate were analyzed by flow cytometry. The proteins expression of XPO1,p-AKT,AKT,Cleaved Caspase-3,p21 were determined by Western blot. Fluorescence microscope was used in observing the intracellular location of XPO1. 【Results】 KPT- 8602 inhibited the growth of U937 cells in a dose- dependent(P<0.001)and time- dependent manner(P<0.001),but normal PBMC were unaffected. 48 h after treatment with KPT-8602,a higher proportion of cells in G1 phase was observed(P<0.001)and the apoptosis rate increased(P=0.016)with drug concentration in U937 cells. XPO1 protein expression of U937 cells was significantly higher than normal PBMC(P=0.003). 48 h after treatment with KPT- 8602,the protein expression of XPO1 decreased(P=0.011),p-AKT decreased(P=0.011),and Cleaved Caspase- 3 increased(P=0.009). In addition,the protein expression of p21,the cargo protein of XPO1,increased in both the nuclei and the cytoplasm(P<0.05)after treatment with KPT- 8602. XPO1 decreased in both the nuclei and the cytoplasm under the fluorescence microscope after treatment with KPT- 8602.【Conclusion】KPT- 8602 can inhibit the proliferation of U937 cells,block the cell cycle at G1 phase,and induce cell apoptosis,which may partially be attributed to the down-regulation of XPO1 and inhibition of PI3K/AKT signaling.

16.
Chinese Journal of Disease Control & Prevention ; (12): 517-521, 2019.
Article in Chinese | WPRIM | ID: wpr-778705

ABSTRACT

Objective To investigate whether elevated baseline levels of high sensitivity C-Reactive Protein (hsCRP) and neutrophil (NE) are associated with an increased risk of breast cancer in Kailuan female cohort. Methods Females from Kailuan cohort (2006-2007) were included in this study. Information on check-up, hsCRP and NE were collected at baseline for all subjects. Multivariable Cox proportional hazards regression models were used to calculate hazard ratios (HR) and 95% confidence intervals (95%CI) of association between baseline hsCRP and NE values and breast cancer risk. Results By December 31, 2015, a total of 18 866 participants were enrolled in this study. During the follow-up, 183 new cases of breast cancer were observed. All participants were divided into three groups according to the level of hsCRP (3 mg/L). The cumulative incidence of breast cancer were 829/105, 1 211/105 and 1 495/105 in these 3 groups, respectively ( 2=12.08, P=0.002). Compared with participants with lower hsCRP levels (3 mg/L) levels had significantly increased risk of breast cancer (HR=1.71,95%CI: 1.18-2.47, P=0.005), howerver, we didn’t find the statistically significant association between NE level (0.05). Conclusions Elevated levels of hsCRP at baseline might increase the risk of breast cancer in females.

17.
Journal of Pharmaceutical Practice ; (6): 147-155, 2018.
Article in Chinese | WPRIM | ID: wpr-790853

ABSTRACT

Objective To compare the cost-effectiveness of Danshenchuanxiongqin injection versus salvianolate injection for angina pectoris.Methods Databases including CBM,CNKI,VIP,Cochrane library,Pubmed,Embase and Wanfang were electronically searched for the randomized controlled trials(RCTs)on the comparison between Danshen Chuanxiongqin injection and Dsnshenduofensuanyan injection.Literatures were screened and data were extracted in accordance with the inclusion and exclusion criteria.Clinical efficacy of the two drugs were obtained through indirect Meta-analysis.Economic evaluation was carried out using the cost-effectiveness analysis,direct medical cost data included the cost of conventional treatment,drug,ex-amination and hospital expenses.Results Through indirect Meta-analysis,the improvement efficiency of angina were 89.51%and 92.52% for Danshenchuanxiongqin injection and salvianolate injection.The success rates of ECG were 81.40% and 84·05% for two drugs. The costs of Danshenchuanxiognqin injection and salvianolate injection were 4 099.71 and 5 410.16 CNY.The Cost-effectiveness ratio of angina improve of two drugs were 45.80 and 58.48,while the ECG improve were 50.36 and 64.37.And the sensitivity analysis results were stable.Conclusion Compared to salvianolate injection,salvi-anolate injection was probably cost-effective for angina pectoris.

18.
Chinese Journal of Primary Medicine and Pharmacy ; (12): 1174-1177, 2018.
Article in Chinese | WPRIM | ID: wpr-807429

ABSTRACT

Objective@#To investigate the effect of atorvastatin combined with Quick Heart-Reliever Pill on blood fat and serum inflammatory factors of elderly patients with coronary artery disease.@*Methods@#184 elderly patients with coronary artery disease were selected, and they were randomly divided into control group and observation group according to the digital table, 92 cases in each group.The control group was given atorvastatin treatment, while the observation group was given atorvastatin combined with Quick Heart-Reliever Pill.The levels of serum inflammatory factors(TNF-α, CRP, MMP-9 and IL-6) and blood lipid(TC, TG, HDL-C, LDL-C) in the two groups after 1 month treatment were compared.@*Results@#After 1 month treatment, the blood lipid levels(TC, TG, HDL-C, LDL-C) of the observation group were significantly lower than those of the control group[(4.26±0.68)mmoL/L vs.(5.14±0.61)mmoL/L, (1.51±0.17)mmoL/L vs.(1.75±0.19)mmoL/L, (1.60±0.38)mmoL/L vs.(1.52±0.43)mmoL/L, (3.01±0.32)mmoL/L vs.(3.18±0.42)mmoL/L, t=2.774, 2.355, 1.976, 2.476, all P<0.05], and the levels of serum inflammatory factors(TNF-α, CRP, MMP-9 and IL-6) of the observation group were significantly lower than those of the control group[(50.35±7.47)ng/L vs.(64.83±8.96)ng/L, (46.50±5.93)mg/L vs.(57.77±6.48)mg/L, (35.44±6.17)μg/L vs.(46.85±7.44)μg/L, (16.84±6.72)ng/L vs.(26.85±9.42)ng/L, t=11.905, 12.306, 22.159, 8.297, all P<0.05].@*Conclusion@#Atorvastatin combined with Quick Heart-Reliever Pill in the treatment of elderly patients with coronary heart disease can achieve notable effects without increasing the liver burden, which can also effectively refine the blood lipid in patients, reduce the inflammatory factors levels, and it is worthy of clinical promotion.

19.
Chinese Journal of Postgraduates of Medicine ; (36): 793-796, 2018.
Article in Chinese | WPRIM | ID: wpr-700290

ABSTRACT

Objective To observe the clinical effect of tongqiaoliyan traditional Chinese medicine compound atomize combined with VitalStim electric stimulation in patients with dysphagia caused by stroke. Methods Sixty patients with dysphagia caused by stroke from July 2016 to December 2017 were divided into electric stimulation group, atomize group and combine group by random digits table method with 20 cases each. The patients of 3 groups were treated according to different etiologies, then the patients in electric stimulation were given the VitalStim electric stimulation, the patients in atomize group were given tongqiaoliyan traditional Chinese medicine compound atomize, and the patients in combine group were given tongqiaoliyan traditional Chinese medicine compound atomize combined with VitalStim electric stimulation. The dysphagia was evaluated by drinking water test, and the curative effect was evaluated. Results The dysphagia after treatment in 3 groups was significantly improved. The improvement of dysphagia in combine group was significantly better than that in electric stimulation group and atomize group, and there was statistical difference (P<0.05). The total effective rate in combine group was significantly higher than that in electric stimulation group and atomize group: 95.0%(19/20) vs. 70.0% (14/20) and 75.0% (15/20), and there was statistical difference (P<0.05); and there was no statistical difference between electric stimulation group and atomize group (P>0.05). During the treatment period, none of the patients in 3 groups had obvious adverse effects. Conclusions Tongqiaoliyan traditional Chinese medicine compound atomize combined with VitalStim electric stimulation can improve the dysphagia caused by stroke, and the curative effect is better than that of monotherapy. There is no obvious adverse reaction, and the operation is convenient and the patient is easy to accept.

20.
Journal of Sun Yat-sen University(Medical Sciences) ; (6): 73-81, 2018.
Article in Chinese | WPRIM | ID: wpr-712916

ABSTRACT

[Objective]Examine the expression of microsomal prostaglandin E synthase-1(mPGES-1)and nuclear transcription factor kappa B p65(NF-κB p65)in diffuse large B cell lymphoma(DLBCL),assessing their correlation with clinical variables,prognosis and potential clinical valve.[Methods]The immunohistochemistry was uesd to investigate the expression of mPGES-1 and NF-κB p65 in 83 DLBCL patiens'tissues.The relationship between these two proteins and the clinical variables and prognosis of these patients was evaluated.[Results]The high expression of mPGES-1 and NF-κB p65 were observed in 65.1%(54/83)and 73.5%(61/83)cases of DLBCL,respectively.The expression level of NF-κB p65 was positively correlated with mPGES-1 expression(P<0.05).The expression of these two proteins was found to be significantly associated with B cell lymphoma/leukemia-2 protein(BCL-2),higher expression of Ki67,higher lactate dehydrogenase (LDH),more extranodal lesions,advanced Ann Arbor stage and higher international prognostic index(IPI)score(P<0.05). In addition,NF-κB p65 was related with multiple myeloma oncogene 1(MUM1),pathological type(P<0.05). Both mPGES-1 and NF-κB p65 overexpression was correlated with worse overall survival(OS)while NF-κB p65 was an in-dependent prognostic factor for OS of DLBCL(P<0.05).[Conclusions]mPGES-1 and NF-κB p65 were highly expressed in DLBCL and closely linked with each other. The overexpression of mPGES-1 and NF-κB p65 was correlated with tumor progression and unfavorable prognosis in patients with DLBCL.

SELECTION OF CITATIONS
SEARCH DETAIL