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1.
Pakistan Journal of Pharmaceutical Sciences. 2018; 31 (3 Supp.): 1013-1020
in English | IMEMR | ID: emr-198711

ABSTRACT

Lectin has attracted attention because of its ability to serve as a carrier for targeted drug delivery. Large lectins isolated from marine invertebrates and crustaceans have strong immunogenicity and adverse effects, which limit their usefulness. This study reports the identification of catesbeianalectin via screening a bullfrog skin cDNA library. The catesbeianalectin polypeptide has a molecular weight of 1.47 kD, making it the smallest known lectin in terms of molecular weight. Circular dichroism analysis showed a PPII helix secondary structure. Catesbeianalectin strongly induces agglutination of rabbit erythrocytes and a variety of pathogens include Staphylococcus aureus, Streptococcus suis type 2, Actinobacillus pleuropneumoniae, and piglet paratyphoid Salmonella. The mean serum titer in catesbeianalectin-immunized Balb/c mice was 1:25, which was significantly lower than that of positive controls immunized with wheat germ agglutinin. Surface plasmon resonance indicated an S-type lectin. 125I-labeled catesbeianalectin did not pass the blood-brain barrier. This study provides a basis for further research on the potential of catesbeianalectin as a carrier in targeted drug delivery

2.
Article in Chinese | WPRIM | ID: wpr-698205

ABSTRACT

Transoral incisionless fundoplication (TIF)has been used for the treatment of gastroesophageal reflux disease (GERD)via repairing the gastroesophageal junction valve. Both the clinical report and control study confirmed that,with the prerequisite of rational selection of patients,TIF as an emerging technology can effectively cure or improve the clinical symptoms of GERD,esophagitis,and level of esophageal acid exposure. Compared with the traditional treatment,TIF is more effective,safe and having lasting effect for the rationally selected GERD patients. This article reviewed advances in study on TIF for the treatment of GERD.

3.
Chinese Medical Journal ; (24): 2991-2997, 2016.
Article in English | WPRIM | ID: wpr-230843

ABSTRACT

<p><b>OBJECTIVE</b>The arrival of precision medicine plan brings new opportunities and challenges for patients undergoing precision diagnosis and treatment of malignant tumors. With the development of medical imaging, information on different modality imaging can be integrated and comprehensively analyzed by imaging fusion system. This review aimed to update the application of multimodality imaging fusion technology in the precise diagnosis and treatment of malignant tumors under the precision medicine plan. We introduced several multimodality imaging fusion technologies and their application to the diagnosis and treatment of malignant tumors in clinical practice.</p><p><b>DATE SOURCES</b>The data cited in this review were obtained mainly from the PubMed database from 1996 to 2016, using the keywords of "precision medicine", "fusion imaging", "multimodality", and "tumor diagnosis and treatment".</p><p><b>STUDY SELECTION</b>Original articles, clinical practice, reviews, and other relevant literatures published in English were reviewed. Papers focusing on precision medicine, fusion imaging, multimodality, and tumor diagnosis and treatment were selected. Duplicated papers were excluded.</p><p><b>RESULTS</b>Multimodality imaging fusion technology plays an important role in tumor diagnosis and treatment under the precision medicine plan, such as accurate location, qualitative diagnosis, tumor staging, treatment plan design, and real-time intraoperative monitoring. Multimodality imaging fusion systems could provide more imaging information of tumors from different dimensions and angles, thereby offing strong technical support for the implementation of precision oncology.</p><p><b>CONCLUSION</b>Under the precision medicine plan, personalized treatment of tumors is a distinct possibility. We believe that multimodality imaging fusion technology will find an increasingly wide application in clinical practice.</p>


Subject(s)
Humans , Multimodal Imaging , Methods , Neoplasms , Diagnosis , Precision Medicine , Methods
4.
Acta Physiologica Sinica ; (6): 239-247, 2015.
Article in English | WPRIM | ID: wpr-255952

ABSTRACT

There are at least three conserved protein folds shared by ion channel-targeted neurotoxins and antimicrobial defensins, including cysteine-stabilized α-helix and β-sheet fold (CSαβ), inhibitor cystine knot fold (ICK) and β-defensin fold (BDF). Based on a combined data of sequences, structures and functions, it has been proposed that these neurotoxins could originate from related ancient antimicrobial defensins by neofunctionalization. This provides an ideal system to study how a novel function emerged from a conserved structural scaffold during evolution. The elucidation of functional novelty of proteins not only has great significance in evolutionary biology but also will be helpful in guiding rational molecular design. This review describes recent progresses in origin of neurotoxins, focusing on the three conserved protein scaffolds.


Subject(s)
Defensins , Chemistry , Evolution, Molecular , Neurotoxins , Chemistry , Protein Structure, Secondary
5.
Singapore medical journal ; : 645-648, 2013.
Article in English | WPRIM | ID: wpr-337841

ABSTRACT

<p><b>INTRODUCTION</b>According to the findings of some studies, instability due to inertia during changes in speed may negatively impact the quality of chest compressions performed during cardiopulmonary resuscitation (CPR) in a moving environment. This study thus aimed to introduce a simple device that maintains the balance of a person performing CPR in a moving environment, such as an ambulance. We also sought to evaluate the effectiveness of this device in the improvement of the quality of chest compressions.</p><p><b>METHODS</b>The experiment comprised a total of 40 simulated cardiopulmonary arrest scenes (20 in the experimental group and 20 in the control), in which CPR was conducted by eight paramedics. Each simulation involved two paramedics randomly selected from the eight. The ambulance took the same route from the simulated site to the hospital, and continuous CPR was performed on a manikin in the ambulance with or without the aid of our proposed novel device.</p><p><b>RESULTS</b>The average number of chest compressions per simulation in the experimental and control groups was 1330.75 and 1266.60, respectively (p = 0.095). The percentage of chest compressions with adequate depth achieved in the experimental and control groups was 72% ± 4% and 50% ± 3%, respectively (p < 0.0001).</p><p><b>CONCLUSION</b>By maintaining the balance of the CPR performer, our proposed novel device can offset the negative impact that instability (due to a moving environment) has on chest compressions. The device may also lead to an increase in the percentage of chest compressions that achieve adequate depth.</p>


Subject(s)
Adult , Allied Health Personnel , Ambulances , Cardiopulmonary Resuscitation , Methods , Emergency Medical Services , Methods , Equipment Design , Equipment Safety , Female , Humans , Male , Manikins , Movement , Out-of-Hospital Cardiac Arrest , Mortality , Therapeutics , Physical Exertion , Physiology , Reference Values , Risk Assessment , Sensitivity and Specificity , Survival Rate , Treatment Outcome
6.
Chinese Journal of Hematology ; (12): 103-106, 2011.
Article in Chinese | WPRIM | ID: wpr-353534

ABSTRACT

<p><b>OBJECTIVE</b>To improve the recognition of Fechtner syndrome.</p><p><b>METHODS</b>The clinical and laboratory data and family survey of a patient with Fechtner's syndrom was reported.</p><p><b>RESULTS AND CONCLUSION</b>Giant platelets, thrombocytopenia and characteristic granulocyte inclusion bodies (Döhle-like bodies) were found in both peripheral blood and bone marrow smears of the patient. Clinically the patient had renal damage, nervous deafness, and vitreous lesions. There was a family genetic tendency on family survey the diagnosis of Fechtner syndrome is established.</p>


Subject(s)
Hearing Loss, Sensorineural , Genetics , Humans , Male , Middle Aged , Molecular Motor Proteins , Genetics , Mutation , Myosin Heavy Chains , Genetics , Nephritis, Hereditary , Genetics , Thrombocytopenia , Genetics
7.
Microbiology ; (12)2008.
Article in Chinese | WPRIM | ID: wpr-686335

ABSTRACT

A fungus CQ31 isolated from soil samples was identified as Chaetomium sp.. This strain produced effectively xylanases utilizing several liguocellulosic materials in the solid-state fermentation (SSF), and corn straw was the best carbon source. The results of single-factor-experiment showed that the corn straw as the carbon source, tryptone as the nitrogen source, initial moisture content of 80% and initial pH 9.0 were the optimal conditions for xylanase production. Under the optimized conditions, it produced xylanase which was 4897 U/g dry substrate while mannanase was 803 U/g dry substrate after 7 days of cultivation. Therefore, xylanase and mannanase production by Chaetomium sp. CQ31 in SSF possess potential for commercial applications.

8.
Article in Chinese | WPRIM | ID: wpr-320238

ABSTRACT

<p><b>OBJECTIVE</b>To explore the effect of Jiawei Yupingfeng Powder (JYP) on T-lymphocyte subsets in patients with senile chronic bronchitis in acute onset stage (SCB-AOS).</p><p><b>METHODS</b>Patients were divided, according to the randomized controlled principle, into two groups, the 44 patients in the treated group and the 40 in the control group. The conventional western medicinal therapy was given to both groups, but to the treated group, JYP was administered additionally. The therapeutic course to them was 14 days. Changes of CD3, CD4, CD8 and the ratio of them were observed.</p><p><b>RESULTS</b>Increase of CD3, CD4 and CD4/CD8 (P < 0.05) and decrease of CD8 (P < 0.01) were significantly shown in the treated group after treatment, but no change in the control group. Besides, comparison of the total effective rate in the two groups also showed significant difference (P < 0.05).</p><p><b>CONCLUSION</b>Cellular immune function disturbance exists in patients with SCB-AOS, JYP could enhance the efficacy by way of modulating cellular immune function.</p>


Subject(s)
Adjuvants, Immunologic , Therapeutic Uses , Adult , Aged , Aged, 80 and over , Bronchitis, Chronic , Drug Therapy , Allergy and Immunology , CD3 Complex , Blood , CD4-CD8 Ratio , Drugs, Chinese Herbal , Therapeutic Uses , Female , Humans , Male , Middle Aged , Phytotherapy , Powders , T-Lymphocyte Subsets , Allergy and Immunology
9.
Article in Chinese | WPRIM | ID: wpr-685566

ABSTRACT

Since haemoplasmodium is not detected for screening in blood donor,morbidity of transfusion-associated malaria increased rapidly in China recently and became an important problem of public health.It is necessary to emphasize donor screening for malaria.Hematology analyzer with MAPSS is recommended to screen malaria.Flow cytometry and immunochromatography test(ICT)can also screen malaria effectively.For the suspicious samples with haemoplasmodium,it is necessary to be reexamined by standard microscopic examination or polymerase chain reaction(PCR).

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