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1.
Article in Chinese | WPRIM | ID: wpr-940718

ABSTRACT

Esophageal cancer is a digestive tract malignancy with high morbidity and mortality and mainly occurs in males. The 5-year survival rate is lower than 20%. In China, the morbidity and mortality of esophageal cancer rank the first in the world, seriously threatening national health. The pathogenesis of esophageal cancer is diverse, which is generally considered as the consequence of environmental-genetic-gene interaction. In addition to genetic factors and regional characteristics, gene mutation, RNA interference, DNA damage repair, tumor microenvironment, dietary habit, chronic adverse stimulation, and inflammatory reaction are all involved in the occurrence and development of esophageal cancer. However, there is no unified and accurate conclusion. Clarifying the exact pathogenesis of esophageal cancer is of great significance for its early screening, diagnosis, prevention, treatment, and prognosis. Surgery, radiotherapy, and chemotherapy are the three effective methods for the treatment of esophageal cancer. However, due to the atypical early symptoms, most patients have missed the best operation period when diagnosed, resulting in poor clinical prognosis. Moreover, radiotherapy and chemotherapy will cause side effects such as loss of appetite, low immune function, esophagitis, pneumonia, and malnutrition, which is not conducive to the prognosis and treatment maintenance of patients. With definite efficacies on esophageal cancer, traditional Chinese medicine (TCM), which is flexible and diverse in the treatment, can primarily or alternatively be involved in the treatment of esophageal cancer. TCM can eliminate postoperative complications and postoperative infections and relieve adverse gastrointestinal reactions, weakened immune function, and organ damage caused by radiotherapy and chemotherapy. It can enhance clinical efficacy and improve the quality of life of patients. Therefore, it is necessary to systematically summarize the clear pathogenesis or risk factors of esophageal cancer and review the clinical characteristics of TCM in the prevention and treatment of esophageal cancer to facilitate the early screening, diagnosis, and treatment of esophageal cancer and promote the application of TCM in the prevention and treatment of esophageal cancer and related adverse reactions.

2.
Clinical Medicine of China ; (12): 62-67, 2022.
Article in Chinese | WPRIM | ID: wpr-932145

ABSTRACT

Objective:To analyze the changes of metabolic indexes and other characteristics in patients with primary aldosteronism (PA) and improve the understanding of PA.Methods:From January 2017 to August 2020, 91 PA patients who were clearly diagnosed and met the inclusion conditions in Tangshan workers Hospital, Hebei Medical University were selected as the observation object (PA group), and 112 patients diagnosed as essential hypertension (EH) in the same period were included as the control (EH group). A retrospective case-control study was conducted to compare the differences of blood glucose, blood lipid, homocysteine, blood potassium, creatinine, uric acid, aldosterone, renin, plasma aldosterone to renin ratio (ARR), blood pressure and waist circumference between the two groups. The measurement data of normal distribution were compared by independent sample t-test. The non normal distribution data are represented by M (Q 1, Q 3), and the rank sum test is used for inter group comparison. Comparison of counting data χ2 inspection. Logistic regression was used in multivariate analysis. Results:Univariate analysis showed that serum potassium, renin and ARR were (3.37±0.39) mmol/L, 1.61 (0.34, 7.23) ng/L and 96.85 (26.06, 506.10) in PA group and (3.91±0.59) mmol/L, 12.81 (1.90, 82.45) ng/L and 13.22 (1.06, 54.63) in EH group ( t=3.35, z=6.24, z=55.40, all P<0.001). In PA group, systolic blood pressure was (190.80±20.30) mmHg, diastolic blood pressure was (117.70±12.89) mmHg, waist circumference was (91.67±9.38) cm, and in EH group, systolic blood pressure was (177.01±12.89) mmHg, diastolic blood pressure was (101.39±9.34) mmHg, waist circumference was (86.59±9.07) cm. There were significant differences between the two groups ( t=5.88, 10.44, 3.90; all P<0.001). Multivariate logistic regression analysis showed that waist circumference (OR=1.065, 95% CI 1.007-1.118, P<0.001), systolic blood pressure (OR=1.053, 95% CI 1.034-1.077, P<0.001), diastolic blood pressure (OR=1.054, 95% CI 1.031-1.077, P<0.001), and ARR (OR=1.170, 95% CI 1.115-1.228, P<0.001) were the risk factors for PA. Conclusion:Compared with essential hypertension, PA patients have higher blood pressure, ARR and waist circumference, lower blood potassium and renin levels. Systolic blood pressure, diastolic blood pressure, ARR and waist circumference are the risk factors of PA. Strengthening the screening and treatment of PA in high-risk groups is helpful to better avoid the risk of target organ damage.

3.
Article in Chinese | WPRIM | ID: wpr-878940

ABSTRACT

Based on the clinical characteristics of chronic atrophic gastritis in traditional Chinese and Western medicine, the domestic and foreign relevant literature reports and animal models of chronic atrophic as well as the clinical diagnostic indicators of traditional Chinese and western medicine, chronic atrophic gastritis evaluation standard was summarized to evaluate and analyze the coincidence degree of clinical symptoms of the existing chronic atrophic gastritis animal models. The statistical results found that modeling methods with a higher coincidence degree with the existing chronic atrophic gastritis animal models are disease and syndrome combination mode-ling, surgical modeling, multifactor comprehensive modeling and MNNG modeling. Although the animal models were reproduced by such methods as etiology, pathogenesis and disease and syndrome combination similar to those of human beings, there is still a big gap with the natural disease state. Further in-depth studies and improvement shall be made in clinical practice in the hope to provide refe-rence for clinical practice and experimental studies of chronic atrophic gastritis.


Subject(s)
Animals , China , Drugs, Chinese Herbal , Gastritis, Atrophic , Humans , Medicine , Medicine, Chinese Traditional , Models, Animal
4.
Article in Chinese | WPRIM | ID: wpr-908905

ABSTRACT

Clinical pharmacy is an important part of hospital pharmacy. Education of clinical pharmacy is one of the most important tasks in clinical pharmacy personnel training. At present, there is a considerable progress in the development of clinical pharmacy education in China, however, the professional quality of clinical pharmacists is still insufficient and there is a gap between the disciplinary education and clinical practice. In this article, we discuss the origin, development, academic degree, curriculum, teaching methods and practice certification of clinical pharmacy education in China and in United States, in order to explore the applicable methods of clinical pharmacy education in China. It will provide ideas and references for the optimization of the training of clinical pharmacy professionals, accelerating the development of clinical pharmacy in China.

5.
Article in Chinese | WPRIM | ID: wpr-906188

ABSTRACT

Objective:To analyze the research status of the external use of medicinal materials and decoction pieces in the 2020 edition of <italic>Chinese Pharmacopoeia</italic> (volume 1), so as to provide reference for the clinical external use research of Chinese medicines. Method:The external function, usage, dosage and other information of medicinal materials and decoction pieces in the 2020 edition of <italic>Chinese Pharmacopoeia</italic> (volume 1) were analyzed and compared. Result:In the 2020 edition of <italic>Chinese Pharmacopoeia</italic> (volume 1), there were 165 kinds of Chinese medicines that could be used externally, including 25 kinds of Chinese medicines that clearly recorded their functions for external use, such as Euphorbiae Semen, Euphorbiae Semen Pulveratum, Arisaematis Rhizoma, Arisaematis Rhizoma Preparatum, Crotonis Fructus, Crotonis Semen Pulveratum, Nardostachyos Radix et Rhizoma, Artemisiae Argyi Folium, Alumen, Stemonae Radix, Genkwa Flos, Cera Flava, Mel, Psoraleae Fructus, Gardeniae Fructus, Calomelas, Bruceae Fructus, Drynariae Rhizoma, Euphorbiae Ebracteolatae Radix, Cuscutae Semen, Phytolaccae Radix, Meretricis Concha, Talcum, Talci Pulvis and Propolis. The amount of Chinese medicine for external use recorded in the 2020 edition of <italic>Chinese Pharmacopoeia</italic> was mostly appropriate for external use, the amount for external use was not clear. The application method for external use focused on beating powder and applying the affected areas, decocting for fumigation and washing, simmering to paste, etc. Clinical external use was used for the treatment of eczema, tinea pedis, chapped skin and other skin diseases. Conclusion:The number of Chinese medicines recorded in the 2020 edition of <italic>Chinese Pharmacopoeia</italic> for external use is increasing, but the function, dosage and usage of these Chinese medicines for external use still need to be improved.

6.
Article in Chinese | WPRIM | ID: wpr-905947

ABSTRACT

Endoplasmic reticulum (ER) is an important organelle responsible for protein, steroid, lipid and carbohydrate synthesis and calcium-dependent signal transduction in eukaryotic cells. ER homeostasis is essential for normal cell function. ER homeostasis imbalance can induce ER stress (ERS), which participates in the occurrence and development of diseases of the digestive system, respiratory system, circulatory system, nervous system, reproductive system, and endocrine system, and affects body health. Among various diseases, cancers seriously endanger people′s health due to its high mortality rate, disability rate, and recurrence rate. Due to the survival characteristics of unlimited proliferation, tumor cells are often exposed to various internal and external stimuli such as hypoxia, ischemia, excessive proliferation, and starvation, which destroy intracellular protein balance and induce ERS to some extent for survival. ERS plays a major role in various tumors and has dual functions in the survival of tumor cells: promoting the survival of tumor cells by activating a series of adaptive responses, while inducing ERS-related apoptosis pathways, so as to promote tumor cell death and inhibit tumor growth and invasion. As multiple functions of ERS in tumors are reported, many scholars have tried to intervene in the progress of tumors from the perspective of ERS. The therapeutic effect of traditional Chinese medicine (TCM) on tumors has been widely recognized. TCM can participate in the regulation of tumors from many aspects, including ERS, chemoradiotherapy resistance, gastrointestinal adverse reactions caused by chemotherapy, postoperative recurrence and metastasis. Since there are few reports on the antitumor effect of TCM from the perspective of ERS, this paper expounds the influence of ERS on tumorigenesis and development and the progress of TCM intervention in tumor through ERS, in order to provide a new direction for tumor treatment.

7.
Article in Chinese | WPRIM | ID: wpr-872942

ABSTRACT

The pathogenesis of chloasma is complex and diverse. Traditional Chinese medicine (TCM) believes that it is mostly related to liver, spleen and kidney, with hematogenous impassability as the standard and pigmentation on the face or body surface as the main symptom. Western medicine believes that it is closely related to family inheritance, ethnic differences and ultraviolet radiation. At present, there are various clinical therapies, mainly including prevention of ultraviolet radiation, local whitening agent and chemical skin. In addition, TCM plays an important role in the treatment of chloasma, often involving internal use, topical use, acupuncture and moxibustion. Modern studies have shown that TCM therapies mainly regulate the metabolism of antioxidant, endocrine and melanin in vivo. Although the mechanism of action could not explained to some extent, there are still some restriction in the discussions on the mechanism of external use of TCM in controlling chloasma. As the skin nerve-endocrine-immune (NEI) network is proposed and further studied, the role of NEI network in realizing overall functional regulation with cytokines, hormones and neurotransmitters as information molecules has been widely verified and recognized. This paper symmetrically reviewed the pathogenesis of chloasma and the progress of the regulatory effect of TCM, and proposed the possible local efficacy of TCM for external use in treating chloasma by regulating surface NEI network. This is worth further study and exploration in the expectation of providing new ideas for the treatment of chloasma and the studies on the mechanisms of action of TCM for external use.

8.
Article in Chinese | WPRIM | ID: wpr-828056

ABSTRACT

Knee osteoarthritis(KOA) is a common orthopedic disease. Its main symptoms include pain in the medial knee, aggravated knee pain during walking, limited movement, and joint deformity, which seriously affect the daily life of patients. The replication of animal models consistent with clinical symptoms is the premise of KOA-related experimental research. Based on the clinical characteristics of KOA and Western medicine, this paper discusses the existing KOA animal model, and analyzes the clinical anastomosis of the existing model based on the clinical diagnostic criteria of KOA. Through analysis and comparison, it can be seen that most of the existing modeling methods are single-factor animal models, which have a certain gap with the characteristics of KOA caused by clinical multi-factors and interaction. Moreover, modeling methods are mostly guided by Western medicine, and traditional Chinese medicine pathogenic factors are absent in the modeling process. Only cold stimulation method takes cold and wet into account. In the evaluation of the model, knee swelling, pain and articular rickets were the main evaluation criteria, which were different from the diagnostic criteria of Chinese and Western medicine in KOA. In terms of syndrome differentiation and classification, KOA is divided into kidney vacuity and deficiency of marrow, Yang vacuity and cold coagulation, and blood stasis and arrest in traditional Chinese medicine. The exis-ting animal models often confuse KOA with each other. Only cold stimulation method is used to establish KOA of Yang vacuity and cold coagulation. Therefore, in this paper, based on KOA clinical symptoms between Chinese and Western medicine features, analysis of the existing animal models, for further improving KOA animal models, the standardization of the model to evaluate suggestions, in order to improve the animal models and clinical inosculation between Chinese and Western medicine, the KOA animal model to better serve the scientific research work, promote the related mechanism, pathological changes and drug screening.


Subject(s)
Animals , Disease Models, Animal , Humans , Medicine, Chinese Traditional , Osteoarthritis, Knee
9.
Article in Chinese | WPRIM | ID: wpr-771493

ABSTRACT

Homology of medicine and food is an important content in Chinese medicine and also works as the basis for guiding the development of compound health food containing Chinese materia medica. The top products,supplements,health care prescriptions,and medicinal meals in traditional herbal texts are the theoretical treasures of Chinese medicine compound health foods. With the implementation of the National Healthy China 2030,China's major health industry faces with tremendous opportunities. It is necessary to develop a batch of compound health food containing Chinese materia medica with Chinese medicine characteristics,in line with the needs of the country and society. Domestic research on compound health food containing Chinese materia medica mainly focuses on the extraction of functional components,preparation molding processes,quality standards,and efficacy evaluation. However,there are still some deficiencies in the related characteristics of traditional Chinese medicine(TCM) theory and function,evaluation criteria of efficacy and safety,new product R&D evaluation system and R&D platform. Based on a large number of previous studies by this laboratory,the views in nature,flavor and efficacy relationship were put forward in this paper. Based on the establishment of the Chinese medicine function-pharmacology-clinical application database system,the Chinese medicine compatibility database system,the Chinese medicine nature and flavor modern research database system,and the evaluation platform for animal models of Chinese medicine; the efficacy study,safety evaluation system,new product research and development evaluation system as well as research and development platform were established,providing a basis for the development and evaluation of compound health food containing Chinese materia medica. The modern scientific connotation of the core efficacy of compound health food containing Chinese materia medica was explained as well,helpful to promote the research and development of compound health food containing Chinese materia medica and play an important role in general health.


Subject(s)
China , Data Mining , Food , Materia Medica , Medicine, Chinese Traditional
10.
Article in Chinese | WPRIM | ID: wpr-802518

ABSTRACT

Objective: To explore the effect of Zingiberis Rhizoma Recens(ZRR) on hemorrhoids in mice and rats. Method: Sixty SPF-grade SD rats were divided into blank group, model group, Ma Yinglong Shexiang hemorrhoid ointment group (7.5 g·kg-1), and large and small-dose ZRR paste groups (10, 5 g·kg-1). ZRR paste was applied in Yongquan acupoint to observe the effect of 0.05 mL injection with 75% acetic acid solution on hemorrhoids induced by subcutaneous anus in rats, the levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), nitric oxide (NO) in the serum were detected by enzyme linked immunosorbent assay(ELISA), and the rectal histopathology was detected by hematoxylin-eosin(HE). Sixty SPF-grade KM mice were divided into blank group, model group, MA Ying-long Shexiang hemorrhoid ointment group (7.5 g·kg-1), and large and small-dose ginger essential oil groups (0.06, 0.03 mL, three times a day). ZRR paste was applied in crissum to observe the effect of injection of 20% acetic acid solution 0.05 mL (maintaining for 1 min) on hemorrhoids in mice induced by anus. The degree of local swelling ulcer around the anus and score was observed, levels of IL-1β, TNF-α in the serum were determined by ELISA, and the rectal histopathology was detected by HE staining. Result: In the experimental study on treating hemorrhoids with ZRR paste applied on Yongquan point of rats, compared with normal group, serum levels of IL-1β, IL-6, TNF-α, NO in model group were significantly higher (Pβ, IL-6, TNF-α, NO were decreased in each administration group (PPPPβ, TNF-α in model group were significantly higher (PPβ, TNF-α levels (PPConclusion: External application of ZRR can effectively inhibit perianal swelling and ulcer degree, with a good therapeutic effect on hemorrhoids model in rats and mice.

11.
Article in Chinese | WPRIM | ID: wpr-802517

ABSTRACT

Objective: To observe the effect of Sanshencao essential oil on the model of thromboangiitis obliterans in rats. Method: Male Wistar rats were randomly divided into five groups according to body weight:sham operation group, model group, Tongsaimai group (1.05 g·kg-1), large and small-dose Sanshencao essential oil groups (0.125 mL·cm-2, twice a day, 0.125 mL·cm-2, once a day), with 10 in each group. The model of occlusive vasculitis was established by injecting sodium laurate solution (10 g·L-1) into the left lower limb artery of rats. Sham operation group was injected with the same amount of normal saline at the same position. Large and small doses of Sanshencao essential oil were applied directly to the affected area, and the remaining groups were given corresponding drugs or pure water for 10 days. One hour after the last administration, the pathological changes were graded; the levels of thromboxane B2(TXB2), interleukin-1 (IL-1), 6-oxo-prostaglandinF1α (6-K-PGF1α), endothelin (ET) were measured by radioimmunoassay. Hematoxylin-eosin(HE) staining was used to observe the pathological changes of the femoral arteries in the left lower limbs. Result: Compared with sham operation group, the degree, extent, inflammation and thrombosis of the affected limbs in model group increased significantly (PPPP2, ET and IL-1 in serum (PP1α (PPConclusion: Sanshencao essential oil has a good therapeutic effect on thrombosis angiitis obliterans model in rats, which related to its anti-oxidation and alleviating inflammation of vascular wall.

12.
Article in Chinese | WPRIM | ID: wpr-802516

ABSTRACT

Objective: To study the effect of Yinhuangshen essential oil in mice of solar dermatitis model by ultraviolet (UVB). Method: Sixty SPF BALB/c female mice were randomly divided into blank group, model group, Minlixiao group, and large and small-dose Yinhuangshen essential oil groups. Except for blank group, model group was given 0.25 mL·cm-2 normal saline on the back every day. The Minlixiao group was given Min Lixiao every day. Low-dose Yinhuangshen essential oil group was given 0.125 mL·cm-2, and large-dose group was given 0.25 mL·cm-2, once a day, and the corresponding drugs were given continuously for 8 days. Except for blank group, all of the other groups were irradiated for 1.5 hours at 15 cm away from 308 nm UVB lamp on the 6th day. One hour and 24 hours after irradiation, mice were given corresponding drug once, and skin was observed 48 hours later. Contents of nitric oxide (NO), nitric oxide synthase (NOS) and tumor necrosis factor-alpha (TNF-α) in some local skin homogenates were measured. The histopathological changes were observed by htoxylin eosin(HE) staining. Result: Compared with blank group, skin of model group was reddish, swollen and ulcerated, epithelium and adnexal were absent, a large number of inflammatory cells and necrosis were adhered to the surface, the local histopathological changes significantly (Pα were significantly increased (Pα in the skin tissue of Minlixiao group and Yinhuangshen essential oil group were significantly decreased, and it could remarkable reduce the pathological changes in local tissue morphology (PConclusion: Yinhuangshen essential oil has a good effect in preventing and treating solar dermatitis.

13.
Article in Chinese | WPRIM | ID: wpr-802515

ABSTRACT

The external treatment of traditional Chinese medicine(TCM) is the earliest known treatment method for diseases. It originated in pre-Qin dynasty, grew up in Han and Tang dynasties, innovated in Song and Yuan dynasties, and developed in Ming and Qing dynasties. It has made outstanding contributions to the human health.With the rapid rise of modern drug-derived diseases, the external treatment of drugs has attracted wide attention at home and abroad due to its small adverse reactions, convenient application and accurate curative effect. With the increasing of TCM for external use drugs, dosage form, method, the clinical application of TCM has been expanding unceasingly, it has been widely applied to the pediatric, gynecological, internal medicine, oncology, however, there has not many specifications for amount, usage, time, and course of treatment, which are more arbitrary. Topical use of TCM can play a direct role in the treatment of local lesions. The external use of TCM is mainly explained by "the principle of external treatment is the principle of internal treatment", and the internal use function can only be explained from the theoretical part of TCM on its external treatment characteristics, and most can not well guide the corresponding clinical application of TCM. There are few reports on the mechanism of external use of TCM, and most of them are from theory to theory, lacking the confirmation and support of modern studies. There are also related reports on transdermal absorption of scattered TCM for external use. However, most of the external use of TCM are crude preparations such as pulverising, decocting in water and alcohol extraction, and its more random which the dose, dosage type, course of treatment, external use methods, coupled with its complex ingredients and low content, it is difficult to explain the mechanism of external use simply from transdermal absorption, nor does it meet the facts of clinical application of TCM. The mechanism of external use is not clear, and restricted the development of TCM for external use. This article through the extensive experimental research, literature research and database research, and proposed that TCM for external use through adjustment of neural-endocrine-immune network disorders, through local micro effect, micro stimulation, micro absorption, that's the "three fine tuning balances", and exert external use curative effect, so as to achieve the purpose of treating diseases.

14.
Article in Chinese | WPRIM | ID: wpr-802514

ABSTRACT

With the rapid rise of modern drug-derived diseases, the external treatment has received extensive attention at home and abroad, due to its small adverse reactions, convenient application and accurate efficacy.At present, although there are many clinical applications of Chinese medicine external treatment, there are few unified norms and strong arbitrariness in related external treatment techniques, for example, external methods, dosage forms, means, dosage, time, etc.As a result, the development of Chinese medicine external treatment is slow and chaotic, and it can not serve the clinic well.It also hinders the development and expansion of external treatment techniques.This paper discusses the current status of external treatment techniques, and clarifies the development trend of Chinese medicine external treatment, in order to pointing out the direction for the development of Chinese medicine external treatment, it will promote the standardization of Chinese medicine external treatment techniques, and it will be better servicing the clinical practice.

15.
Article in Chinese | WPRIM | ID: wpr-802136

ABSTRACT

Objective: To observe the effect of Prunellae Spica extracts (PS) on the lipid metabolism in Zuker Diabetes Fatty (ZDF) rats based on AMP-activated protein kinase/acetyl CoA carboxylase (AMPK/ACC) signaling pathway.Method: The 32 male ZDF (fa/fa) type 2 diabetic rats were randomly divided into model group,metformin group (180 mg·kg-1·d-1),and low and high-dose PS groups (12.25,24.5 mg·kg-1·d-1),with 8 in each group.8 male Zuker Lean (ZL) rats were selected as normal group.Body weight and fasting blood glucose were monitored at the 0th,4th and 8th weeks after administration.After 8 weeks,abdominal aorta blood was collected,serum was frozen at-20℃ by centrifugation,liver tissue was frozen at-80℃,fixed with 4% paraformaldehyde and embedded in paraffin.Serum triglyceride (TG),cholesterol (CHO),low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA) levels were measured by radioimmunoassay.Fat droplets in hepatocytes were measured by oil red O staining.Gene expressions of AMP-activated protein kinase-alpha 2(AMPKα2),Acetyl CoA carboxylase (ACC) in liver were detected by real-time polymerase chain reaction (Real-time PCR).Protein expressions of p-AMPKα were observed by immuno-histochemical (IHC) method.Result: Compared with the normal group,the T2DM model group showed significant increases in serum levels of TG,CHO,LDL-C,FFA and lipid droplets in hepatocytes.AMPKα2 mRNA expression was decreased,while ACC1 and ACC2 mRNA expressions were increased significantly.p-AMPKα protein expression in liver was decreased significantly (PPα2,down-regulation in mRNA expressions of ACC1 and ACC2,and up-regulation in protein expression of p-AMPKα(PPConclusion:PS can effectively improve liver lipid metabolism in ZDF rats.Its mechanism may be related to the regulation of AMPK/ACC signaling pathway in liver.

16.
Article in Chinese | WPRIM | ID: wpr-802089

ABSTRACT

With a clear efficacy in external treatment of diseases, traditional Chinese medicine(TCM) has been widely applied in surgery, in acute, pediatric, gynecological, internal medicine and other departments. Its advantages include easy use, direct access to lesions, and prevent of the first-pass effect of the liver. However, its mechanism of action is still unclear, which impacts its clinical promotion. This article makes analysis and summary on the clinical application of TCM in external treatment of diseases, the characteristics of commonly used TCM in treatment of diseases, and the relationship of the common externally treated diseases and the "neurological-endocrine-immune network". The findings showed that TCM for external treatment were widely used, with exact curative effects; the pathogenesis of commonly used TCM for externally treating diseases is closely related to the "neuro-endocrine-immune network". Moreover, the modern pharmacological effects of TCM for externally treating diseases were found to be 100%related to the "neuro-endocrine-immune network". According to the analysis of the relationship between the common diseases externally treated with TCM and the "neuro-endocrine-immune network", the pathogenesis of the treatment with TCM is closely related to the "neuro-endocrine-immune network". Therefore, we believe that the "neuro-endocrine-immune network" system is probably one of the main mechanisms of action of TCMs for externally treating diseases. The verification of this mechanism requires the joint efforts of various departments in conducting clinical or experimental verification in the future, so as to provide a reference for clarifying the mechanism of TCM for external use, and basis for the promotion and application of TCM for external use.

17.
Article in Chinese | WPRIM | ID: wpr-771658

ABSTRACT

The coincidence of the existing eczema animal model and the clinical characteristics of eczema, and the application characteristics of eczema animal model were analyzed, and the evaluation method and improvement method of corresponding eczema animal model was put forward. Based on the clinical diagnostic criteria of eczema of traditional Chinese medicine and western medicine, the characteristics of current eczema model and the modeling method were summarized, and the anastomosis situations between the existing animal model and the eczema clinical characteristics were analyzed. At present, the back eczema models of mice have been usually used, which reflected the pathophysiological characteristics of clinical eczema to a certain degree. However, the existing animal models fail to reflect the clinical characteristics of traditional Chinese medicine. Future studies focused on establishing animal models that better reflect the clinical characteristics of eczema and the index system of eczema model.


Subject(s)
Animals , Disease Models, Animal , Eczema , Medicine, Chinese Traditional , Mice
18.
Protein & Cell ; (12): 861-877, 2017.
Article in English | WPRIM | ID: wpr-756980

ABSTRACT

Cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells contribute to the body's immune defenses. Current chimeric antigen receptor (CAR)-modified T cell immunotherapy shows strong promise for treating various cancers and infectious diseases. Although CAR-modified NK cell immunotherapy is rapidly gaining attention, its clinical applications are mainly focused on preclinical investigations using the NK92 cell line. Despite recent advances in CAR-modified T cell immunotherapy, cost and severe toxicity have hindered its widespread use. To alleviate these disadvantages of CAR-modified T cell immunotherapy, additional cytotoxic cell-mediated immunotherapies are urgently needed. The unique biology of NK cells allows them to serve as a safe, effective, alternative immunotherapeutic strategy to CAR-modified T cells in the clinic. While the fundamental mechanisms underlying the cytotoxicity and side effects of CAR-modified T and NK cell immunotherapies remain poorly understood, the formation of the immunological synapse (IS) between CAR-modified T or NK cells and their susceptible target cells is known to be essential. The role of the IS in CAR T and NK cell immunotherapies will allow scientists to harness the power of CAR-modified T and NK cells to treat cancer and infectious diseases. In this review, we highlight the potential applications of CAR-modified NK cells to treat cancer and human immunodeficiency virus (HIV), and discuss the challenges and possible future directions of CAR-modified NK cell immunotherapy, as well as the importance of understanding the molecular mechanisms of CAR-modified T cell- or NK cell-mediated cytotoxicity and side effects, with a focus on the CAR-modified NK cell IS.


Subject(s)
Animals , HIV Infections , Allergy and Immunology , Therapeutics , HIV-1 , Allergy and Immunology , Humans , Immunity, Cellular , Immunological Synapses , Immunotherapy , Killer Cells, Natural , Transplantation , Neoplasms , Allergy and Immunology , Therapeutics , Receptors, Antigen, T-Cell , Genetics , Allergy and Immunology , Recombinant Fusion Proteins , Genetics , Allergy and Immunology , T-Lymphocytes , Allergy and Immunology , Transplantation
19.
Chinese Traditional Patent Medicine ; (12): 2045-2049, 2017.
Article in Chinese | WPRIM | ID: wpr-657968

ABSTRACT

AIM To optimize the aqueous extraction for polysaccharides from Astragali Radix and to evaluate the in vitro antitumor activity.METHODS With extraction temperature,extraction time and solid-liquid ratio as influencing factors,extraction rate of polysaccharides as an evaluation index,the extraction was optimized by uniform design.The effect of polysaccharides on the proliferation of non-small cell lung cancer NCI-H460 cells,the apoptosis rate and cell cycle of NCI-H460 cells,and the expressions of Caspase-3,Bax and Bcl-2 were detected by MTT assay,flow cytometry and Western blot,respectively.RESULTS The optimal conditions were determined to be 100 ℃ for extraction temperature,1 h for extraction time,and 1 ∶ 35 for solid-liquid ratio,the extraction rate of polysaccharides was 3.62%.Compared with the control group,the proliferation of NCI-H460 cells was significandy inhibited in a dose-dependent manner (P < 0.01),the S phase ratio,early apoptosis rate,late apoptosis rate and total apoptosis rate were markedly increased (P < 0.01),and the Caspase-3 expression and Bax/Bcl-2 ratio were also obviously increased (P < 0.01) in the polysaccharides group.CONCLUSION This fast,stable and reliable method can be used for the aqueous extraction for polysaccharides from Astragali Radix,which can significantly inhibit the proliferation of NCI-H460 cells and induce apoptosis of NCI-H460 cells.

20.
Article in Chinese | WPRIM | ID: wpr-657361

ABSTRACT

Objective To observe effects of Heyutai Fuzhu Jiangtang Tablets combined with metformin in insulin resistance (IR); To discuss its mechanism of action. Methods 6–7 week old male ZDF (fa/fa) rats were randomly divided into model group,metformin group,Heyutai Fuzhu Jiangtang Tablets group(Jiangtang Tablets group),and metformin combined with Heyutai Fuzhu Jiangtang Tablets group.ZDF(fa/+)rats were chosen as normal group.Each medication group was given relevant medicine for gavage for 6 weeks. Body weight, FBG, TG, TC, FFA, FINS, HOMA-IR, OGTT and HE staining were tested. HE staining was used to observe the pathological changes of skeletal muscle. RT-PCR and Western blot were used to detect skeletal muscle corresponding gene and protein expression. Results Compared with Jiangtang Tablets group and metformin group, TC, FFA, FBG, and HOMA-IR in metformin combined with Heyutai Fuzhu Jiangtang Tablets group decreased significantly (P<0.05, P<0.01). Blood glucose level and AUC significantly decreased at each time point in OGTT. HE staining of skeletal muscle fibers arranged in order; nucleus increased and internal movement was not significant, without obvious infiltration of inflammatory cells. Expressions of skeletal muscle InsR, Akt, and Glut4 mRNA expression increased (P<0.05, P<0.01). Expressions of skeletal muscle p-InsR, p-Akt, and Glut4 protein expression increased (P<0.05, P<0.01). Conclusion Heyutai Fuzhu Jiangtang Tablets combined with metformin can improve IR in type 2 diabetic rats, and the effect is better than single-application.

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