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1.
Article in Chinese | WPRIM | ID: wpr-928021

ABSTRACT

This study explored the protective effect of atractylenolide Ⅰ(AO-Ⅰ) against acetaminophen(APAP)-induced acute liver injury(ALI) in mice and its underlying mechanism. C57 BL/6 J mice were randomly divided into a control group, an APAP group(500 mg·kg~(-1)), a low-dose combination group(500 mg·kg~(-1) APAP + 60 mg·kg~(-1) AO-Ⅰ), and a high-dose combination group(500 mg·kg~(-1) APAP + 120 mg·kg~(-1) AO-Ⅰ). ALI was induced by intraperitoneal injection of APAP(500 mg·kg~(-1)). AO-Ⅰ by intragastric administration was performed 2 hours before APAP treatment, and the control group received the same dose of solvent by intragastric administration or intraperitoneal injection. The protective effect of AO-Ⅰ against APAP-induced ALI was evaluated by detecting alanine aminotransferase(ALT) and aspartate aminotransferase(AST) levels in the plasma and H&E staining in liver tissues of mice. The malondialdehyde(MDA) and glutathione(GSH) content and catalase(CAT) activity in mouse liver tissues were detected to evaluate the effect of AO-Ⅰ on APAP-induced oxidative stress in the liver. The proteins in the liver p38 mitogen-activated protein kinase(p38 MAPK), c-jun N-terminal kinase(JNK), and nuclear factor kappa-B p65(NF-κB p65) signaling pathways were measured by Western blot, and the liver inflammatory cytokines interleukin-1β(IL-1β) and interleukin-6(IL-6) were detected by real-time PCR. Compared with the APAP group, the combination groups showed reduced APAP-induced ALT level and liver MDA content, potentiated liver CAT activity, and elevated GSH content. Mechanistically, AO-Ⅰ treatment significantly inhibited APAP-up-regulated MAPK phosphorylation and NF-κB p65, and significantly reduced the transcriptional activities of IL-1β and IL-6, downstream targets of NF-κB p65. AO-Ⅰ can improve APAP-induced ALI and the underlying mechanism is related to the inhibition of the MAPK/NF-κB p65 signaling pathway in APAP-challenged mice.


Subject(s)
Acetaminophen/adverse effects , Animals , Chemical and Drug Induced Liver Injury/drug therapy , Lactones , Mice , NF-kappa B/metabolism , Sesquiterpenes , Signal Transduction
2.
Article in Chinese | WPRIM | ID: wpr-773155

ABSTRACT

Tanshinone Ⅱ_A( Tan Ⅱ_A),the liposoluble constituents of Salvia miltiorrhiza,can not only ameliorate the lipidic metabolism and decrease the concentration of lipid peroxidation,but also resist oxidation damage,scavenge free radicals and control inflammation,with a protective effect on prognosis after liver function impairment. Therefore,the studies on the exact mechanism of Tan Ⅱ_A in protecting the liver can provide important theoretical and experimental basis for the prevention and treatment effect of Tan Ⅱ_A for liver injury. In the present study,the protective effects and mechanism of Tan Ⅱ_A on 4-hydroxynonenal( 4-HNE)-induced liver injury were investigated in vitro. Normal liver tissues NCTC 1469 cells were used to induce hepatocytes oxidative damages by 4-HNE treatment. The protective effect of Tan Ⅱ_A on hepatocytes oxidative damages was detected by release amount of lactate dehydrogenase( LDH) analysis and hoechst staining. The protein expression changes of peroxisome proliferator-activated receptor α( PPARα) and peroxisome proliferator response element( PPRE) were analyzed by Western blot analysis in NCTC 1469 cells before and after Tan Ⅱ_A treatment. The gene expression changes of fatty aldehyde dehydrogenase( FALDH) were analyzed by Real-time polymerase chain reaction( PCR) analysis. The results showed that 4-HNE increased the release amount of LDH,lowered the cell viability of NCTC 1469 cells,and Tan Ⅱ_A reversed 4-HNE-induced hepatocyte damage. Western blot analysis and RT-PCR analysis results showed that 4-HNE decreased the expression of PPARα and FALDH and increased the expression of 4-HNE. However,the expression of PPARα and FALDH were increased significantly and the expression of 4-HNE was decreased obviously after Tan Ⅱ_A treatment. This study confirmed that the curative effect of Tan Ⅱ_A was obvious on hepatocytes damage,and the mechanism may be associated with activating PPARα and FALDH expression as well as scavenging 4-HNE.


Subject(s)
Aldehyde Oxidoreductases , Metabolism , Aldehydes , Animals , Cell Line , Abietanes , Pharmacology , Hepatocytes , Lipid Peroxidation , Mice , Oxidative Stress , PPAR alpha , Metabolism
3.
Article in Chinese | WPRIM | ID: wpr-851324

ABSTRACT

Objective: To study the chemical constituents in the twigs and leaves of Ervatamia officinalis. Methods: The chemical constituents were isolated from the total alkaloids of the twigs and leaves of Ervatamia officinalis by using silica gel, ODS, Sephadex LH-20, and HPLC. The chemical structures of the compounds were identified based on their physical and chemical properties and spectroscopic methods. Results: Fourteen alkaloids were isolated and identified as 7S-coronaridine hydroxyindolenine (1), 7S-voacangine hydroxyindolenine (2), coronaridine (3), 19S-heyneanine (4), voacangine (5), tabernaemontanine (6), dregamine (7), 3-(2-oxopropyl) coronaridine (8), 3-oxovoacangine (9), voastrictine (10), 16R,19E-isositsirikine (11), 16R,19Z-isositsirikine (12), 16R,19E-isositsirikine N4-oxide (13), and geissoschizol (14). Conclusion: Compounds 8-14 are obtained from this plant for the first time.

4.
Article in Chinese | WPRIM | ID: wpr-777936

ABSTRACT

Objective To assess the prevalence of overweight and obesity among residents aged 35 years or older in Yuhuan county of Zhejiang Province and explore the associations of overweight and obesity with hypertension. Methods A cross-sectional study was conducted among 124 693 permanent residents aged 35 years or older, who were randomly selected using cluster sampling method from Yuhuan county. Results The prevalence of overweight and obesity was 34.53% and 10.18% among subjects, respectively. The overweight prevalence for male (35.56%) was higher than that for female (33.66%), whereas the obesity prevalence for female (11.26%) was higher than that for male (8.90%) (all P<0.05). The multivariate analysis showed that age, education, occupation, regular physical exercise, smoking, drinking and high-salt diet were possible factors for overweight and obesity (all P<0.05). After adjustment for covariates, compared to participants with normal weight, the multivariate logistic regression indicated that the adjusted odds ratio (OR) and their 95% confidential interval of overweight and obesity were 2.02 (95% CI:1.93-2.11) and 3.83 (95% CI:3.57-4.10) for hypertension in men, and were 1.94(95% CI:1.86-2.02)and 3.56(95% CI:3.35-3.77) in women, respectively. Conclusions The prevalence of overweight and obesity was high among residents aged 35 years or older in Yuhuan county, and they may relate to the increasing risk of hypertension.

5.
International Eye Science ; (12): 1295-1297, 2018.
Article in Chinese | WPRIM | ID: wpr-695432

ABSTRACT

·AIM: To observe the efficacy of 25G+ vitrectomy surgery for malignant glaucoma after cataract surgery. · METHODS: Totally 18 eyes of 18 patients with malignant glaucoma after cataract surgery were analyzed retrospectively from January 2013 to June 2016. The visual acuity was between hand moving to 0. 5 before surgery, the intraocular pressure was between 18. 3-56. 8mmHg before surgery, an average of 35. 21 ± 10. 43mmHg. The length eye axis was between 19. 60-22. 46mm, an average of 20. 63 ± 0. 48mm. The depth of anterior chamber was between 0. 98 - 1. 86mm, an average of 1. 31 ± 0. 22mm. All the patients were performed with 25G + vitrectomy. The visual acuity, anterior chamber and intraocular pressure were studied after treatment. ·RESULTS: The patients were followed up for 6-18mo with an average of 9mo. BCVA at the last follow up improved to 0. 2-0. 8, and there was significant difference compared to that before operation (P<0. 01). IOP was from 12. 3-19. 8mmHg, an average of 16. 05±2. 46mmHg, there was significant difference compared to that before operation ( t = 7. 59, P<0. 01 ). The depth of anterior chamber was between 1. 89-3. 49mm, an average of 2. 42±0. 47mm, there was significant difference compared to that before operation(t=9. 07, P<0. 01). Only one case had IOP of 8mmHg, after treatment the IOP was 15mmHg. No complications such as corneal endothelium decompensation, intraocular lens ( IOL ) capture, intraocular hemorrhage, infection and uncontrolled IOP were observed. · CONCLUSION: The 25G + vitrectomy is safe and effective for treating malignant glaucoma, controls IOP and reduces complications compared with traditional vitrectomy.

6.
International Eye Science ; (12): 690-693, 2018.
Article in Chinese | WPRIM | ID: wpr-695281

ABSTRACT

·AIM: To discuss the curative effect of extra-panretinal photocoagulation on proliferative diabetic retinopathy (PDR) during vitreous operation. ·METHODS:We conducted a retrospective analysis of 70 diabetic retinopathy patients (70 eyes) who accepted vitreous surgery in our hospital due to vitreous hemorrhage or vitreous retina traction change from March 2011 to March 2013. In follow-up clinics, according to the difference of patient's retinal photo-coagulation range, we divided it into extra - panretinal photocoagulation group (40 patients) and panretinal photocoagulation (30 patients),respectively observed the best corrected visual acuity,intraocular pressure and retinal vessel changes in two groups and made statistical analysis. ·RESULTS:Through comparing the eye condition,visual acuity after 3mo, intraocular pressure between the two groups patients, the difference had no statistical significance (all of the P>0.05). Comparing the incidence of retinal blood vessel leak, posterior pole exudate, and posterior pole bleeding points in two groups patients,the difference had statistically significant (all of the P<0.05). Comparing the incidence of no-perfusion area of retina, prepapillary vascular leak in two groups patients, the difference had statistically significant(P = 0. 04, 0. 02). Comparing the incidence of macular edema in two groups patients after operation,the difference had no statistically significance(P=1.00). Comparing the extinction time of macular edema,the difference had statistically significant (P<0.05), extra-panretinal photocoagulation group was better than panretinal photocoagulation.· CONCLUSION: To cure proliferation diabetic retinopathy, adopting the extra - panretinal photocoagulation has a better curative effect than panretinal photocoagulation on curing no-perfusion area of retina, retinal blood vessel leak, prepapillary vascular leak in operation on vitreous. However, the excessive retinal photocoagulation has a clear damage on retina and choroid. Therefore, in the treatment, while covering the retinopathy as far as possible, it is important to master the laser energy and spot number preventing the complications.

7.
International Eye Science ; (12): 1254-1257, 2017.
Article in Chinese | WPRIM | ID: wpr-641292

ABSTRACT

AIM: To explored the treatment effects of retinal laser photocoagulation and the applications of color Doppler flow imaging(CDFI) in the diabetic retinopathy.METHODS: We collected 60 patients(120 eyes) with diabetic retinopathy(Ⅲ~Ⅳstage) from February 2013 to February 2014 in Anyang Eye Hospital admitted in fundus disease department.The health control (HC) group of 55 normal people was established simultaneously.Ocular blood flow velocity of the 55 normal people (110 eyes) and the 60 patients (120 eyes) was examined by CDFI of central retinal artery (CRA) and posterior ciliary artery (PCAs) using the PHILIPS HD6.Peak systolic velocity(PSV) was recorded.The examinations of CDFI, electroretinogram(ERG) and the vision were performed in pre-operation, 1,7d, 1, 3 and 6mo postoperatively.RESULTS: Compared the PSV of CRA of the DR group before and after photocoagulation to the data of the HC group, there was a significant difference(P0.05).Compared the aA and bA of ERG of the DR group before and after photocoagulation to the data of the HC group, there was a significant difference(P0.05).The vision of 49 cases(98 eyes) was improved 1 to 3 rows, the effective rate was 82%.The vision of 11 cases(22 eyes) remained constant.CONCLUSION: Retinal laser photocoagulation is an effective treatment to diabetic retinopathy patients, which can significantly reduce the peak systolic velocity of the central retinal artery, improve and stabilize the condition, protect visual function in long-term clinical effect.Color doppler flow imaging can observe the changes of ocular vessel flow velocity in diabetic` eyes noninvasively, repeatedly and in real time, providing a basis for clinical treatments.

8.
Journal of Preventive Medicine ; (12): 1119-1122, 2016.
Article in Chinese | WPRIM | ID: wpr-792563

ABSTRACT

Objective To explore the relationship between pulse blood pressure and cognitive function for the elders in Yuhuan County,Zhejiang Province.Methods Multi -stage cluster method was used to select 1 700 residents aged over 60 years in Yuhuan County,Zhejiang Province.Mini -Mental State Examination (MMSE)was used to screen cognitive impairment.Locally weighted scatter plot smoothing was used to explore the local linear trend between MMSE score and diastolic.The association between pulse pressure or blood pressure and cognitive function was analyzed by multivariate logistic regression analysis.Results The prevalence of cognitive impairments for the elders aged over 60 years in Yuhuan County was 15.74%.There was significant difference between the pulse blood pressure of cognitive impairment group (64.01 ±16.31)mmHg and none -cognitive impairment group (58.97 ±14.67)mmHg.The t value was 4.97.Pulse blood pressure and the elderly MMSE score showed a significant positive correlation.Multivariate logistic regression analysis showed that cognitive function related to diastolic blood pressure (OR =1.010,95% CI:1.001 -1.019,P =0.034)after adjusting several covariate.Conclusion Normal cognitive function was associated with decreased pulse blood pressure among the elders aged over 60 years in Yunhuan County,Zhejiang Province.

9.
Article in Chinese | WPRIM | ID: wpr-635742

ABSTRACT

Background Retinopathy of prematurity is mainly due to retinal neovascularization.Objective This laboratory work was to evaluate the efficacy of different dosage of avastin for inhibiting retinal neovascularization.Methods Ninety 7-day-old clean C57BL/J6 mice were randomized into six groups as follows:air control group,hyperxia control group,hyperxia BSS group and avastin groups.C57BL/J6 mice in air control group were raised in regular air environments.The fifty mice were fed under the environment with 75% ±2% oxygen for 5 days to establish the retinal neovascularization models.The 1.25,2.50 and 5.00 g/L avastin (0.5 μl) were injected inteavtreally in forty-five mice models as low,moderate and high dosage avastin groups respectively,and 0.5 μl BSS was used at the same way in fifteen models as hyperxia BSS group.The mice were sacrificed in the 17-day-old age using excessive anesthesia method and the retina sections were prepared for the calculation of the numbers of vascular endothelial cell nuclei broken retinal inner membrane after hemotoxylin and eosin staining.The expression of CD34 in the retina was detected by immunochemistry.The morphology and distribution of retinal neovascular vessel in various groups were observed using retinal flat.The use of the animals followed the Regulations for the Administration of Affairs Concerning Experimental Animals by State Science and Technology Commission.Results The numbers of cell nuclei broken the inner limiting membrane was significant increased in the hyperxia group compared with the air control group( P<0.01 ),and those in difference doses of avastin were considerably reduced in comparison with hyperxia BSS group (P<0.01) and hyperxia group (P<0.01 ).The decrease of numbers of cell nuclei broken the inner limiting membrane was obvious in low dose of high dose of avastin compared with low dose of avastin (P<0.05 ).CD34 was positively expressed in retina internal membrane of hyperxia group.Retinal flat revealed the regular distribution and normal structure of retinal vessels in air control group and avastin groups.However,retinal and vitreous cavity neovascularization,leakage and enlarged non-perfusion regions in the perimeter of the retina were seen in hyperxia group and hyperxia BSS group. Conclusions Intravitreal injection of avastin can arrest retinal angiogenesis in oxygen-induced retinal neovascularization models in a dose-dependent manner.

10.
National Journal of Andrology ; (12): 182-186, 2009.
Article in Chinese | WPRIM | ID: wpr-292401

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the mechanism of Longchang Granule in the treatment of benign prostatic hyperplasia.</p><p><b>METHODS</b>Rat models of prostate hyperplasia were made by castration and testosterone propionate injection. After treated respectively with Longchang Granule and Longbishu by gastrogavage for 30 days, all the model rats were killed and their prostate glands harvested for the measurement of the wet weight and detection of the expression of bax in the prostatic hyperplastic tissues by RV 2-step method.</p><p><b>RESULTS</b>The wet weight of the prostate was (0.61 +/- 0.03) g in the blank control group, (0.95 +/- 0.04) g in the model group, (0.73 +/- 0.02) g in the Longbishu group, (0.80 +/- 0.05) g in the low-dose Longchang group, (0.78 +/- 0.07) g in the medium-dose Longchang group and (0.68 +/- 0.03) g in the high-dose Longchang group, with significant differences between the model and the intervention groups (P < 0.05). The prostate indexes in the above groups were 0.143 +/- 0.006, 0.226 +/- 0.008, 0.172 +/- 0.004, 0.199 +/- 0.012, 0.181 +/- 0.010 and 0.168 +/- 0.003, respectively, and the expressions of bax by mean optical density were 0.226 +/- 0.010, 0.184 +/- 0.005, 0.206 +/- 0.015, 0.199 +/- 0.001, 0.202 +/- 0.003 and 0.211 +/- 0.003, respectively, both with significant differences between the model and the intervention groups (P < 0.05).</p><p><b>CONCLUSION</b>Longchang Granule can effectively reduce the wet weight of the prostate and alleviate its pathological changes in BPH rats, the mechanism of which may be associated with its effect of upregulating the expression of bax and accelerating cell apoptosis in the prostate tissues.</p>


Subject(s)
Animals , Apoptosis , Drugs, Chinese Herbal , Therapeutic Uses , Male , Phytotherapy , Prostatic Hyperplasia , Drug Therapy , Metabolism , Rats , Rats, Wistar , Testosterone , bcl-2-Associated X Protein , Metabolism
11.
Article in English | WPRIM | ID: wpr-296007

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the attenuating effect of curcumin, an anti-inflammatory compound derived from dietary spice turmeric (Curcuma longa) on the pro-inflammatory insulin-resistant state in 3T3-L1 adipocytes.</p><p><b>METHODS</b>Glucose uptake rate was determined with the [3H] 2-deoxyglucose uptake method. Expressions of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) were measured by quantitative RT-PCR analysis and ELISA. Nuclear transcription factor kappaB p65 (NF-kappa p65) and mitogen-activated protein kinase (MAPKs) were detected by Western blot assay.</p><p><b>RESULTS</b>The basal glucose uptake was not altered, and curcumin increased the insulin-stimulated glucose uptake in 3T3-L1 cells. Curcumin suppressed the transcription and secretion of TNF-alpha and IL-6 induced by palmitate in a concentration-dependent manner. Palmitate induced nuclear translocation of NF-kappaB. The activities of Jun NH2-terminal kinase (JNK), extracellular signal-regulated kinase1/2 (ERK1/2) and p38MAPK decreased in the presence of curcumin. Moreover, pretreatment with SP600125 (inhibitor of JNK) instead of PD98059 or SB203580 (inhibitor of ERK1/2 or p38MAPK, respectively) decreased the up-regulation of TNF-alpha induced by palmitate.</p><p><b>CONCLUSION</b>Curcumin reverses palmitate-induced insulin resistance state in 3T3-L1 adipocytes through the NF-kappaB and JNK pathway.</p>


Subject(s)
3T3-L1 Cells , Animals , Anthracenes , Pharmacology , Anti-Inflammatory Agents, Non-Steroidal , Pharmacology , Curcumin , Pharmacology , Glucose , Metabolism , Insulin , Pharmacology , Insulin Resistance , Interleukin-6 , Genetics , Metabolism , JNK Mitogen-Activated Protein Kinases , Metabolism , MAP Kinase Signaling System , Mice , NF-kappa B , Metabolism , Palmitates , Pharmacology , Protein Kinase Inhibitors , Pharmacology , Tumor Necrosis Factor-alpha , Genetics , Metabolism , Up-Regulation
12.
Article in Chinese | WPRIM | ID: wpr-282368

ABSTRACT

<p><b>OBJECTIVE</b>To study whether the argyrophil nucleolar organizer regions (AgNOR) in T lymphocytes of peripheral blood in coke-oven workers can be used as a biomarker of effect for polycyclic aromatic hydrocarbon (PAH) exposure.</p><p><b>METHODS</b>Fifty-two male coke-oven workers were divided into three groups according to exposure levels of coke oven emissions: high-exposure, middle-exposure and low-exposure workers. Additionally 10 men without occupational PAH exposure were chosen as control group. Peripheral blood T lymphocytes were cultured, spread on slides and stained with silver nitrate. The ratio of AgNOR area vs. nuclear area (I/S) in T lymphocytes was analyzed. Urinary 1-hydroxypyrene (1-OHP) was measured as the internal dose of PAH exposure.</p><p><b>RESULTS</b>Mean urinary 1-OHP level in high-exposure group (16.56 +/- 2.77 micromol/mol Cr) was significantly higher than those in low-exposure group (3.30 +/- 2.77 micromol/mol Cr, P < 0.001) and control group (3.04 +/- 1.58 micromol/mol Cr, P < 0.01). The mean I/S of AgNOR in T lymphocytes in high-exposure group (0.056 +/- 0.010) was significantly lower than those in middle-exposure group (0.065 +/- 0.013, P < 0.05), low-exposure group (0.067 +/- 0.008, P < 0.01) and control group (0.076 +/- 0.007, P < 0.001). It was also found that I/S of AgNOR were significantly decreased in middle-exposure group and low-exposure group in comparison with control group (P < 0.01, P < 0.05).</p><p><b>CONCLUSIONS</b>The occupational exposure to PAH resulted in increase of 1-OHP in urine and decrease of AgNOR in T lymphocytes. PAH exposure might lead to damage T lymphocytes function and AgNOR may be considered as a biomarker of effect for PAH exposure.</p>


Subject(s)
Antigens, Nuclear , Blood , Biomarkers , Blood , Urine , Coke , Poisoning , Humans , Lymphocytes , Cell Biology , Metabolism , Male , Occupational Exposure , Polycyclic Aromatic Hydrocarbons , Poisoning , Pyrenes
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