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1.
Arq. bras. med. vet. zootec ; 67(2): 527-534, Mar-Apr/2015. tab
Article in English | LILACS, VETINDEX | ID: lil-747052

ABSTRACT

We evaluated the inclusion of peeled-mango waste meal as a source of carbohydrate in the protein:carbohydrate ratio (CP:CH) on performance and chemical composition of pacamã (Lophiosilurus alexandri) juveniles. One hundred and fifty fish (11.31±0.96g) were stocked in sixteen 500 L tanks, fed three times daily (10% of live weight), in a system with water recirculation with biofilter. The treatments consisted of four experimental diets with decreasing levels of the ratio between crude protein and carbohydrate (1.40, 0.94, 0.56 and 0.29), with four replications per treatment. At the end of 60 days, we evaluated animal performance (final average weight gain, specific growth rate, total apparent feed intake, carcass yield, survival) and physicochemical composition of the carcass. The protein:carbohydrate ratios affected all performance variables (P<0.05), except for survival (P>0.05). The carcass chemical composition variables were modified, except for mineral matter, pH and moisture. Mango meal can be used at the proportion of up to 15% in the diet for pacamã, establishing a CP:CHO ratio of 1.40 without impairing animal performance and the carcass chemical composition.(AU).


Avaliou-se a inclusão do farelo residual de manga sem casca como fonte de carboidrato na relação proteína:carboidrato (PB:CHO) sobre o desempenho e a composição química da carcaça em juvenis de pacamãs. Foram utilizados 150 peixes (11,31±0,96g) estocados em 16 caixas de 500L, alimentados três vezes ao dia (10% peso vivo), em um sistema de recirculação de água com biofiltro. Os tratamentos consistiram em quatro dietas experimentais com níveis decrescentes da relação entre proteína bruta e carboidrato (1,40; 0,94; 0,56 e 0,29), com quatro repetições cada tratamento. Ao final de 60 dias, foram avaliados o desempenho zootécnico (ganho de peso médio final, taxa de crescimento específico, consumo total de ração aparente, conversão alimentar aparente, rendimento de carcaça, sobrevivência) e a composição físico-química da carcaça. As relações de proteína:carboidrato afetaram todas as variáveis de desempenho (P<0,05), com exceção da sobrevivência (P>0,05). Os valores da composição química da carcaça foram alterados, com exceção da matéria mineral, do pH e da umidade. A farinha de manga pode ser utilizada na proporção de até 15% na ração de pacamã, estabelecendo uma relação 1,40 PB:CHO, sem prejudicar o desempenho zootécnico e a composição química da carcaça.(AU)


Subject(s)
Animals , Body Composition , Catfishes/growth & development , Dietary Carbohydrates , Dietary Proteins , Mangifera
2.
Rev. bras. plantas med ; 17(4,supl.2): 915-921, 2015. tab, graf
Article in Portuguese | LILACS | ID: lil-771162

ABSTRACT

RESUMO Agentes químicos têm sido usados para auxiliar na inibição da formação do biofilme e impedir desenvolvimento da doença periodontal (DP). O objetivo deste estudo foi avaliar radiograficamente a ação do extrato de própolis na progressão da DP induzida em ratos. Foram utilizados 48 ratos Wistar, divididos em 4 grupos (n=12): Controle, Pincel, Própolis e Clorexidina. Os grupos foram subdivididos para análise aos 7 e 21 dias. A progressão da DP foi avaliada radiograficamente pela distância entre a junção cemento-esmalte e a crista óssea alveolar na face mesial do 1º molar inferior. A perda óssea foi significativamente menor nos animais do grupo própolis em ambos os períodos (p ≤ 0,01). Concluiu-se por análise radiográfica que a aplicação tópica do extrato de própolis interfere reduzindo a progressão da doença periodontal induzida por ligadura em ratos, demonstrando a importância desse composto como substância auxiliar no tratamento periodontal.


ABSTRACT Chemical agents have been used to assist on inhibiting biofilm formation and on preventing the development of periodontal disease (PD). The aim of this study was to radiographically evaluate the action of propolis extract on the progression of PD induced in rats. 48 Wistar rats were divided into 4 groups (n = 12): Control, Brush, Propolis and Chlorhexidine. The groups were subdivided for analysis at 7 and 21 days. The progression of the periodontal disease was radiographically assessed by the distance between the cement-enamel junction and the alveolar bone crest mesial of the 1st molar. Bone loss was significantly lower in the Propolis group in both periods (p ≤ 0.01). By radiographic analysis, it is concluded that the topical application of propolis extract interferes by reducing the progression of ligature-induced periodontal disease in rats, demonstrating the importance of this compound as an auxiliary substance in periodontal treatment.


Subject(s)
Rats , Periodontal Diseases/prevention & control , Propolis/analysis , Diagnostic Imaging , Disease Progression , Periodontics/classification
3.
Arq. bras. med. vet. zootec ; 65(5): 1281-1285, out. 2013. ilus, graf, tab
Article in English | LILACS | ID: lil-689742

ABSTRACT

The present report describes the clinical and pathological findings related to diffuse heterotopic hairs associated with pseudo-placentational endometrial hyperplasia in a canine uterus. Macroscopically, the uterus was intensely enlarged and thicker, and the endometrial surface was irregular and covered with black hairs similar to the hairs of the bitch. Histologically, heterotopic hairs associated with pseudo-placentational endometrial hyperplasia were observed.


O presente relato descreve os achados clínicos e patológicos de uma heterotopia de pelos difusa associada com hiperplasia endometrial pseudo-placentacional em um útero de uma cadela. Macroscopicamente, o útero estava intensamente aumentado de volume e espesso, a superfície do endométrio estava irregular e recoberta com pelos pretos semelhantes aos pelos da cadela. Histologicamente, heterotopia de pelos associada com hiperplasia endometrial pseudoplacentacional foi observada.


Subject(s)
Animals , Female , Dogs , Endometrium/growth & development , Endometrium/pathology , Uterus/growth & development , Uterus/pathology
4.
Braz. j. med. biol. res ; 45(12): 1127-1134, Dec. 2012. ilus
Article in English | LILACS | ID: lil-659650

ABSTRACT

DNA repair is crucial to the survival of all organisms. The bacterial RecA protein is a central component in the SOS response and in recombinational and SOS DNA repairs. The RecX protein has been characterized as a negative modulator of RecA activity in many bacteria. The recA and recX genes of Herbaspirillum seropedicae constitute a single operon, and evidence suggests that RecX participates in SOS repair. In the present study, we show that the H. seropedicae RecX protein (RecX Hs) can interact with the H. seropedicaeRecA protein (RecA Hs) and that RecA Hs possesses ATP binding, ATP hydrolyzing and DNA strand exchange activities. RecX Hs inhibited 90% of the RecA Hs DNA strand exchange activity even when present in a 50-fold lower molar concentration than RecA Hs. RecA Hs ATP binding was not affected by the addition of RecX, but the ATPase activity was reduced. When RecX Hs was present before the formation of RecA filaments (RecA-ssDNA), inhibition of ATPase activity was substantially reduced and excess ssDNA also partially suppressed this inhibition. The results suggest that the RecX Hs protein negatively modulates the RecA Hs activities by protein-protein interactions and also by DNA-protein interactions.


Subject(s)
Bacterial Proteins/metabolism , Herbaspirillum/chemistry , Rec A Recombinases/metabolism , DNA, Bacterial , Escherichia coli/metabolism , Protein Binding
5.
Braz. j. med. biol. res ; 45(12): 1135-1140, Dec. 2012. ilus, tab
Article in English | LILACS | ID: lil-659653

ABSTRACT

Azospirillum brasilense is a diazotroph that associates with important agricultural crops and thus has potential to be a nitrogen biofertilizer. The A. brasilense transcription regulator NifA, which seems to be constitutively expressed, activates the transcription of nitrogen fixation genes. It has been suggested that the nitrogen status-signaling protein GlnB regulates NifA activity by direct interaction with the NifA N-terminal GAF domain, preventing the inhibitory effect of this domain under conditions of nitrogen fixation. In the present study, we show that an N-terminal truncated form of NifA no longer required GlnB for activity and lost regulation by ammonium. On the other hand, in trans co-expression of the N-terminal GAF domain inhibited the N-truncated protein in response to fixed nitrogen levels. We also used pull-down assays to show in vitro interaction between the purified N-terminal GAF domain of NifA and the GlnB protein. The results showed that A. brasilense GlnB interacts directly with the NifA N-terminal domain and this interaction is dependent on the presence of ATP and 2-oxoglutarate.


Subject(s)
Adenosine Triphosphate/metabolism , Azospirillum brasilense/enzymology , Bacterial Proteins/metabolism , Ketoglutaric Acids/metabolism , Transcription Factors/metabolism , beta-Galactosidase/metabolism , Azospirillum brasilense/metabolism , Genetic Vectors , Plasmids
6.
Braz. j. microbiol ; 43(2): 661-674, Apr.-June 2012. ilus, graf, tab
Article in English | LILACS | ID: lil-644484

ABSTRACT

The acidic peatlands of southern Brazil are ecosystems essential for the maintenance of the Atlantic Forest, one of the 25 hot-spots of biodiversity in the world. In this work, we investigated the composition of prokaryotic communities in four histosols of three acidic peatland regions by constructing small-subunit (SSU) rRNA gene libraries and sequencing. SSU rRNA gene sequence analysis showed the prevalence of Acidobacteria (38.8%) and Proteobacteria (27.4%) of the Bacteria domain and Miscellaneous (58%) and Terrestrial (24%) groups of Crenarchaeota of the Archaea domain. As observed in other ecosystems, archaeal communities showed lower richness than bacterial communities. We also found a limited number of Euryarchaeota and of known methanotrophic bacteria in the clone libraries.


Subject(s)
Base Sequence , Biodiversity , Ecology , Ecosystem , Environmental Microbiology , Euryarchaeota/genetics , In Vitro Techniques , Wetlands/analysis , Polymerase Chain Reaction/methods , Sequence Analysis , Soil Microbiology , Electrophoresis , Microbiology , Prevalence , Soil
7.
Braz. j. med. biol. res ; 45(2): 113-117, Feb. 2012. ilus, tab
Article in English | LILACS | ID: lil-614577

ABSTRACT

Azospirillum brasilense is a nitrogen-fixing bacterium associated with important agricultural crops such as rice, wheat and maize. The expression of genes responsible for nitrogen fixation (nif genes) in this bacterium is dependent on the transcriptional activator NifA. This protein contains three structural domains: the N-terminal domain is responsible for the negative control by fixed nitrogen; the central domain interacts with the RNA polymerase σ54 co-factor and the C-terminal domain is involved in DNA binding. The central and C-terminal domains are linked by the interdomain linker (IDL). A conserved four-cysteine motif encompassing the end of the central domain and the IDL is probably involved in the oxygen-sensitivity of NifA. In the present study, we have expressed, purified and characterized an N-truncated form of A. brasilense NifA. The protein expression was carried out in Escherichia coli and the N-truncated NifA protein was purified by chromatography using an affinity metal-chelating resin followed by a heparin-bound resin. Protein homogeneity was determined by densitometric analysis. The N-truncated protein activated in vivo nifH::lacZ transcription regardless of fixed nitrogen concentration (absence or presence of 20 mM NH4Cl) but only under low oxygen levels. On the other hand, the aerobically purified N-truncated NifA protein bound to the nifB promoter, as demonstrated by an electrophoretic mobility shift assay, implying that DNA-binding activity is not strictly controlled by oxygen levels. Our data show that, while the N-truncated NifA is inactive in vivo under aerobic conditions, it still retains DNA-binding activity, suggesting that the oxidized form of NifA bound to DNA is not competent to activate transcription.


Subject(s)
Azospirillum brasilense/metabolism , Bacterial Proteins/metabolism , Nitrogen Fixation/genetics , Transcription Factors/metabolism , Azospirillum brasilense/chemistry , Azospirillum brasilense/genetics , Bacterial Proteins/genetics , Bacterial Proteins/isolation & purification , Carrier Proteins/genetics , Carrier Proteins/isolation & purification , Carrier Proteins/metabolism , Transcription Factors/genetics , Transcription Factors/isolation & purification
8.
Braz. j. med. biol. res ; 44(12): 1215-1221, Dec. 2011. ilus, tab
Article in English | LILACS | ID: lil-606546

ABSTRACT

Sugarcane is an important agricultural product of Brazil, with a total production of more than 500 million tons. Knowledge of the bacterial community associated with agricultural crops and the soil status is a decisive step towards understanding how microorganisms influence crop productivity. However, most studies aim to isolate endophytic or rhizosphere bacteria associated with the plant by culture-dependent approaches. Culture-independent approaches allow a more comprehensive view of entire bacterial communities in the environment. In the present study, we have used this approach to assess the bacterial community in the rhizosphere soil of sugarcane at different times and under different nitrogen fertilization conditions. At the high taxonomic level, few differences between samples were observed, with the phylum Proteobacteria (29.6 percent) predominating, followed by Acidobacteria (23.4 percent), Bacteroidetes (12.1 percent), Firmicutes (10.2 percent), and Actinobacteria (5.6 percent). The exception was the Verrucomicrobia phylum whose prevalence in N-fertilized soils was approximately 0.7 percent and increased to 5.2 percent in the non-fertilized soil, suggesting that this group may be an indicator of nitrogen availability in soils. However, at low taxonomic levels a higher diversity was found associated with plants receiving nitrogen fertilizer. Bacillus was the most predominant genus, accounting for 19.7 percent of all genera observed. Classically reported nitrogen-fixing and/or plant growth-promoting bacterial genera, such as Azospirillum, Rhizobium, Mesorhizobium, Bradyrhizobium, and Burkholderia were also found although at a lower prevalence.


Subject(s)
Biota , Bacteria/genetics , Rhizosphere , /genetics , Soil Microbiology , Saccharum/microbiology , Brazil , Bacteria/classification , DNA, Bacterial/genetics , Fertilizers , Nitrogen , Phylogeny , Plant Roots/microbiology
9.
Braz. j. med. biol. res ; 44(5): 394-401, May 2011. ilus, tab
Article in English | LILACS | ID: lil-586513

ABSTRACT

Streptococcus mutans is a Gram-positive bacterium present in the oral cavity, and is considered to be one of the leading causes of dental caries. S. mutans has a glnK gene, which codes for a PII-like protein that is possibly involved in the integration of carbon, nitrogen and energy metabolism in several organisms. To characterize the GlnK protein of S. mutans, the glnK gene was amplified by PCR, and cloned into the expression vectors pET29a(+) and pET28b(+). The native GlnK-Sm was purified by anion exchange (Q-Sepharose) and affinity (Hi-Trap Heparin) chromatography. The GlnK-His-Sm protein was purified using a Hi-Trap Chelating-Ni2+ column. The molecular mass of the GlnK-His-Sm proteins was 85 kDa as determined by gel filtration, indicating that this protein is a hexamer in solution. The GlnK-His-Sm protein is not uridylylated by the Escherichia coli GlnD protein. The activities of the GlnK-Sm and GlnK-His-Sm proteins were assayed in E. coli constitutively expressing the Klebsiella pneumoniae nifLA operon. In K. pneumoniae, NifL inhibits NifA activity in the presence of high ammonium levels and the GlnK protein is required to reduce the inhibition of NifL in the presence of low ammonium levels. The GlnK-Sm protein was unable to reduce NifL inhibition of NifA protein. Surprisingly, the GlnK-His-Sm protein was able to partially reduce NifL inhibition of the NifA protein under nitrogen-limiting conditions, in a manner similar to the GlnK protein of E. coli. These results suggested that S. mutans GlnK is functionally different from E. coli PII proteins.


Subject(s)
Bacterial Proteins/genetics , Gene Expression Regulation, Bacterial/genetics , Nitrogen/metabolism , PII Nitrogen Regulatory Proteins/genetics , Streptococcus mutans/genetics , Bacterial Proteins/metabolism , Chromatography, Affinity , Escherichia coli/genetics , Klebsiella pneumoniae/genetics , Nitrogen Fixation , PII Nitrogen Regulatory Proteins/metabolism , Polymerase Chain Reaction , Streptococcus mutans/metabolism
10.
Braz. j. med. biol. res ; 44(3): 182-185, Mar. 2011. ilus, tab
Article in English | LILACS | ID: lil-576068

ABSTRACT

Herbaspirillum seropedicae is an endophytic diazotrophic bacterium, which associates with important agricultural plants. In the present study, we have investigated the attachment to and internal colonization of Phaseolus vulgaris roots by the H. seropedicae wild-type strain SMR1 and by a strain of H. seropedicae expressing a red fluorescent protein (DsRed) to track the bacterium in the plant tissues. Two-day-old P. vulgaris roots were incubated at 30°C for 15 min with 6 x 10(8) CFU/mL H. seropedicae SMR1 or RAM4. Three days after inoculation, 4 x 10(4) cells of endophytic H. seropedicae SMR1 were recovered per gram of fresh root, and 9 days after inoculation the number of endophytes increased to 4 x 10(6) CFU/g. The identity of the recovered bacteria was confirmed by amplification and sequencing of the 16SrRNA gene. Furthermore, confocal microscopy of P. vulgaris roots inoculated with H. seropedicae RAM4 showed that the bacterial cells were attached to the root surface 15 min after inoculation; fluorescent bacteria were visible in the internal tissues after 24 h and were found in the central cylinder after 72 h, showing that H. seropedicae RAM4 is capable of colonizing the roots of the dicotyledon P. vulgaris. Determination of dry weight of common bean inoculated with H. seropedicae SMR1 suggested that this bacterium has a negative effect on the growth of P. vulgaris.


Subject(s)
Herbaspirillum/growth & development , Phaseolus/microbiology , Plant Roots/microbiology , Colony Count, Microbial , Herbaspirillum/genetics , Microscopy, Confocal , Microscopy, Fluorescence
11.
Arq. bras. med. vet. zootec ; 63(1): 56-60, Feb. 2011. ilus
Article in English | LILACS | ID: lil-582324

ABSTRACT

A 7-month-old miniature poodle female dog was referred with dysphagia. After clinical, radiographic, and endoscopic examination, it was diagnosed a probable case of cricopharyngeal achalasia. The patient underwent surgical treatment and presented normal swallowing and no regurgitation after 24 hours post-surgery. Positive recovery and progressive body weight gain until 180 days after surgery was observed.


Uma cadela da raça poodle miniatura com sete meses de idade foi atendida no Hospital Veterinário apresentando quadro de disfagia. Após exames clínico, radiográfico e endoscópico detalhados, houve diagnóstico provável de acalasia cricofaríngea. A paciente, submetida a tratamento cirúrgico, apresentou deglutição normal e nenhuma regurgitação 24 horas pós-cirurgia. Houve recuperação satisfatória e ganho de peso progressivo até 180 dias após a cirurgia.


Subject(s)
Animals , Dogs/classification , Deglutition Disorders/pathology , Endoscopy/adverse effects , Endoscopy , Radiography
12.
Braz. j. med. biol. res ; 43(5): 431-436, May 2010. ilus, tab
Article in English | LILACS | ID: lil-546332

ABSTRACT

Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) is an important pest for Brazilian sugarcane. In the present study, we detected two distinct spots in hemolymph from septic injured larvae (HDs1 and HDs2), which are separated by 2DE gel electrophoresis. Both spots were subjected to in-gel tryptic digestion and MALDI-TOF/TOF analysis, which revealed the sequence VFGTLGSDDSGLFGK present in both HDs1 and HDs2. This sequence had homology and 80 percent identity with specific Lepidoptera antimicrobial peptides called gloverins. Analyses using the ImageMaster 2D software showed pI 8.94 of the HDs1 spot, which is similar to that described to Hyalophora gloveri gloverin (pI 8.5). Moreover, the 14-kDa molecular mass of the spot HDs1 is compatible to that of gloverins isolated from the hemolymph of Trichoplusia ni, Helicoverpa armigera and H. gloveri. Antimicrobial assays with partially purified fractions containing the HDs1 and HDs2 polypeptides demonstrated activity against Escherichia coli. This is the first report of antimicrobial polypeptides in D. saccharalis, and the identification of these peptides may help in the generation of new strategies to control this pest.


Subject(s)
Animals , Anti-Bacterial Agents/pharmacology , Hemolymph/chemistry , Lepidoptera/chemistry , Peptides/pharmacology , Proteins/analysis , Anti-Bacterial Agents/isolation & purification , Larva/chemistry , Mass Spectrometry , Peptides/isolation & purification , Proteins/metabolism , Sepsis/metabolism
13.
Arq. bras. med. vet. zootec ; 61(4): 825-834, ago. 2009. ilus
Article in Portuguese | LILACS-Express | LILACS | ID: lil-524436

ABSTRACT

Compararam-se duas técnicas cirúrgicas de redução e estabilização da articulação coxofemoral experimentalmente luxada em cães. Dois grupos de animais, submetidos às respectivas técnicas após a indução cirúrgica da luxação, foram acompanhados clínica e radiograficamente por um período de 60 dias, findos os quais, realizaram-se avaliações macroscópica e histológica e teste de tensiometria das articulações. Cada grupo foi constituído por oito animais, clinicamente sadios, com pesos entre 5 e 20kg. Os animais submetidos ao implante de fáscia apresentaram, ao exame físico, evolução da deambulação significativamente precoce em relação aos do grupo submetido ao implante de pino de Steinmann, além de menor grau de atrofia muscular. Os testes de tensiometria, as avaliações macroscópicas e radiográficas e os exames histológicos não diferiram entre os grupos, evidenciando também que ambas as técnicas não geraram alterações deletérias à articulação operada. Conclui-se que a técnica de estabilização da articulação coxofemoral com implante de fascia lata foi clinicamente eficaz e vantajosa quando comparada à técnica do pino transarticular.


It was compared both surgical techniques of reduction and stabilization of experimentally luxated coxofemoral join in dog. Two groups were submitted to the techniques after surgical induction of the luxation. All animals were clinically and radiografically observed during 60 days. After that, a macroscopic study, an histological exam, and a tensiometry test in the articulations were performed. Each group had eight healthy animals, weighting from 5 to 20kg. The most important advantage was related to the deambulation, which the animals submited to the facia lata implant showed a faster evolution after the surgery at the physical exam, and muscular atrophy in a smaller degree. The tensiometry tests, the radiographic and the histological exams did not present important differences between both groups, but they were useful to show that the two techniques did not cause alterations in the studied articulation. It can be concluded that the stabilization of the coxofemoral articulation using bubaline fascia lata implant was clinically efficient and more advantageous compared to the transarticular pin technique.

14.
Braz. j. med. biol. res ; 41(4): 289-294, Apr. 2008. ilus
Article in English | LILACS | ID: lil-479679

ABSTRACT

Azospirillum brasilense is a diazotroph found in association with important agricultural crops. In this organism, the regulation of nitrogen fixation by ammonium ions involves several proteins including the uridylyltransferase/uridylyl-removing enzyme, GlnD, which reversibly uridylylates the two PII proteins, GlnB and GlnZ, in response to the concentration of ammonium ions. In the present study, the uridylylation/deuridylylation cycle of A. brasilense GlnB and GlnZ proteins by GlnD was reconstituted in vitro using the purified proteins. The uridylylation assay was analyzed using non-denaturing polyacrylamide gel electrophoresis and fluorescent protein detection. Our results show that the purified A. brasilense GlnB and GlnZ proteins were uridylylated by the purified A. brasilense GlnD protein in a process dependent on ATP and 2-oxoglutarate. The dependence on ATP for uridylylation was similar for both proteins. On the other hand, at micromolar concentration of 2-oxoglutarate (up to 100 µM), GlnB uridylylation was almost twice that of GlnZ, an effect that was not observed at higher concentrations of 2-oxoglutarate (up to 10 mM). Glutamine inhibited uridylylation and stimulated deuridylylation of both GlnB and GlnZ. However, glutamine seemed to inhibit GlnZ uridylylation more efficiently. Our results suggest that the differences in the uridylylation pattern of GlnB and GlnZ might be important for fine-tuning of the signaling pathway of cellular nitrogen status in A. brasilense.


Subject(s)
Humans , Azospirillum brasilense/metabolism , Bacterial Proteins/metabolism , Azospirillum brasilense/genetics , Bacterial Proteins/genetics , Electrophoresis, Polyacrylamide Gel , Escherichia coli/genetics , Escherichia coli/metabolism , Nucleotidyltransferases , PII Nitrogen Regulatory Proteins/genetics , PII Nitrogen Regulatory Proteins/metabolism , Plasmids/genetics , Signal Transduction
15.
Acta cir. bras ; 20(supl.1): 185-189, 2005.
Article in Portuguese | LILACS | ID: lil-474167

ABSTRACT

PURPOSE: The aim of the present study was confront the results of the cytological examination with hybrid capture II in the diagnosis of induced cervical intraepithelial lesion-HPV, correlating the cytological findings with biomoleculares. METHODS: The research was carried through in a group of 160 sexually active women who had espontaneamente looked its gynecologists for consultation of routine, having been submitted to the collection of cervicovaginal material for cytology and for examination of hybrid capture II in the Centro de Patologia Clínica and the Hospital e Maternidade Promater, in the city of the Natal-RN. RESULTS: The results had shown to relatively high numbers of positive cases for HPV using hybrid capture II (41.87%) and the cytology (23.75%). The agreement between the two studied methods relatively was raised (59.38%). It was evident also that the viruses with high oncogênico potential had presented found in the compatible cytology with Lesion of low risk (11.88%), followed of Lesion of high risk (NIC II and III); already the viruses with low oncogênico potential were more associates the Lesion of low risk (6.25%), followed of Lesion of high risk. CONCLUSION: The cytology, exactly with its limitations, is an important method in the detention of attributable patologias to the HPV, emphasizing that the molecular method comes to complement it and to consolidate the cytological findings.


Subject(s)
Humans , Female , Adolescent , Adult , Cervix Uteri/virology , DNA Probes, HPV , DNA, Viral/analysis , Uterine Cervical Diseases/diagnosis , Vaginal Smears/methods , Papillomavirus Infections/diagnosis , Biopsy , Cervix Uteri/pathology , Uterine Cervical Diseases/genetics , Papillomavirus Infections/genetics , Uterine Cervical Neoplasms/prevention & control , Sensitivity and Specificity
16.
Braz. j. med. biol. res ; 35(6): 651-661, June 2002. ilus, tab
Article in English | LILACS | ID: lil-309516

ABSTRACT

Two Azospirillum brasilense open reading frames (ORFs) exhibited homology with the two-component NtrY/NtrX regulatory system from Azorhizobium caulinodans. These A. brasilense ORFs, located downstream to the nifR3ntrBC operon, were isolated, sequenced and characterized. The present study suggests that ORF1 and ORF2 correspond to the A. brasilense ntrY and ntrX genes, respectively. The amino acid sequences of A. brasilense NtrY and NtrX proteins showed high similarity to sensor/kinase and regulatory proteins, respectively. Analysis of lacZ transcriptional fusions by the ß-galactosidase assay in Escherichia coli ntrC mutants showed that the NtrY/NtrX proteins failed to activate transcription of the nifA promoter of A. brasilense. The ntrYX operon complemented a nifR3ntrBC deletion mutant of A. brasilense for nitrate-dependent growth, suggesting a possible cross-talk between the NtrY/X and NtrB/C sensor/regulator pairs. Our data support the existence of another two-component regulatory system in A. brasilense, the NtrY/NtrX system, probably involved in the regulation of nitrate assimilation


Subject(s)
Azospirillum brasilense , Bacterial Proteins , Gene Expression Regulation, Bacterial , Nitrogen Fixation , Amino Acid Sequence , Azospirillum brasilense , Base Sequence , DNA, Bacterial , Escherichia coli , Genetic Complementation Test , Mutation
17.
Braz. j. med. biol. res ; 29(12): 1599-602, Dec. 1996. ilus
Article in English | LILACS | ID: lil-188440

ABSTRACT

The nitrogenase structural genes (nifHDK) of the endophytic diazotroph Herbaspirillum seropedicae were isolated from a genomic bank by plate hybridization. Sequence analysis of the DNA showed a consensus promoter region upstream from the nifH gene containing a -24/-12 type promoter together with NifA- and integration host factor (IHF)- binding sites. The derived protein sequences of NifH, NifD and NifK contained conserved cysteine residues for binding iron-sulfur clusters and the iron-molybdenum cofactor. These protein sequences showed the strongest similarities to the nifHDK gene products of the symbiotic diazotroph Bradyrhizobium japonicum (93.5 per cent, 91.3 per cent and 83.3 per cent, respectively), the plant-associated diazotrophAzospirillum brasilense (90.0 per cent, 83.7 per cent and 75.1 per cent, respectively) and to Thiobacillus ferrooxidans (91.0 per cent, 83.4 per cent and 81.1 per cent, respectively) of the same phylogenetic group of the protobacteria.


Subject(s)
Nitrogen Fixation , Nitrogenase/genetics , Amino Acid Sequence , Base Sequence , Cysteine/isolation & purification , Genes, Bacterial , Molecular Sequence Data , Open Reading Frames , Sequence Analysis, DNA
18.
Arq. bras. med. vet. zootec ; 48(3): 369-74, jun. 1996. ilus
Article in Portuguese | LILACS | ID: lil-257019

ABSTRACT

This report describes a case of cutaneous transmissible veneral tumor (TVT) in a three-year-old male mongrel dog. Through clinical examination it was observed several cutaneous nodule ranging from aproximately 5mm to 4cm in diameter, some of which ulcerated, and several small and ulcerated nodules on the mucous membrane of the mouth. Through the Fine Meedle Aspiration (FNA) technique, the material of cutaneous nodules was sampled and processed for citologic and karyotypic exams. Upon citology, there was abundance of cells with round nuclei with coarsely granular chromatin and single distinct nucleoli, and cytoplasm containing several vacuoles. The karyotype showed a number of 58 chromosomes. Based on cytological and karyotypic findings extragenital TVT was diagnosed. The dog was treated with vincristine sulfate (Oncovin) in a dose of 0,025 mg/kg/weekly/IV. After the second aplication there was a reduction of 80 per cent of nodules with a complete cure at the end of the treatment


Subject(s)
Animals , Male , Dogs , Venereal Tumors, Veterinary
19.
Rev. microbiol ; 13(2): 173-9, 1982.
Article in Portuguese | LILACS | ID: lil-9912

ABSTRACT

Material de cavidade oral, de 400 criancas, entre 7 a 14 anos, revelou 3% de Entamoeba gingivalis e ausencia de Trichomonas tenax. O estudo envolveu criancas com mucosa oral e dentes sadios (controle), portadoras de alteracoes gengivais e portadores de caries dentarias. Neste trabalho procurou-se relacionar a presenca de Entamoeba gingivalis com o pH bucal e idade dos pacientes. O autor, entre outros achados, obteve maior incidencia de E.gingivalis em criancas com pH bucal 6.0 e idade de 7 anos


Subject(s)
Child , Adolescent , Humans , Male , Female , Entamoeba , Oral Health
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