Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
1.
Article in English | WPRIM | ID: wpr-37555

ABSTRACT

Herpesvirus saimiri (HVS), a member of the gamma-herpesvirus family, encodes an oncoprotein called Saimiri Transforming Protein (STP) which is required for lymphoma induction in non-human primates. However, a detailed mechanism of STP-A11-induced oncogenesis has not been revealed yet. We first report that STP-A11 oncoprotein interacts with TNF-alpha receptor-associated factor (TRAF) 6 in vivo and in vitro. Mutagenesis analysis of the TRAF6-binding motif 10PQENDE15 in STP-A11 reveals that Glu (E)12 residue is critical for binding to TRAF6 and NF-kappaB activation. Interestingly, co-expression of E12A mutant, lack of TRAF6 binding, with cellular Src (Src) results in decreased transcriptional activity of Stat3 and AP-1, a novel target of STP-A11 compared to that of wild type. Furthermore, the presence of STP-A11 enhances the association of TRAF6 with Src and induces the translocation of both TRAF6 and Src to a nonionic detergent-insoluble fraction. Taken together, these studies suggest that STP-A11 oncoprotein up-regulates both NF-kappaB and AP-1 transcription activity through TRAF6, which would ultimately contribute cellular transformation.


Subject(s)
Humans , Transcription, Genetic , Transcription Factor AP-1/agonists , TNF Receptor-Associated Factor 6/metabolism , Solubility , STAT3 Transcription Factor/metabolism , Proto-Oncogene Proteins pp60(c-src)/metabolism , Protein Binding , Oncogene Proteins, Viral/metabolism , NF-kappa B/agonists , Ions , Herpesvirus 2, Saimiriine/metabolism , Detergents , Cell Line
SELECTION OF CITATIONS
SEARCH DETAIL