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1.
The Journal of Practical Medicine ; (24): 2151-2154, 2017.
Article in Chinese | WPRIM | ID: wpr-617111

ABSTRACT

Objective To explore the relationship between polymorphism of miR-499A > G with inci-dence and prognosis of colorectal cancer in Han Chinese. Method 1:1 matched case control study was used to collect samples,and the patients were followed up. The genotypes distribution miR-499A > G were detected by TaqMan probe,and logistic regression model and Cox proportional hazard regression model were used to analysis the relationship between miR-499A>G and incidence and prognosis of colorectal cancer. Results 394 cases and 394 controls were recruited,while miR-499A>G did not increase the risk of colorectal cancer(OR=1.22,95%CI:0.95-1.57)under additive model analyzed by multi-logistic regression model. The means of survival time of AA,AG,GG carriers were 54.19 ± 2.02,44.16 ± 2.59,32.76 ± 5.36 months and there were significant differentia-tion between them in log Rank test(F=11.24,P=0.004). Cox proportional hazard regression model showed that miR-499A > G was significant associated with adverse prognosis of colorectal cancer(HR=1.26,95%CI:1.02-1.57). Conclusion miR-499 A > G has no association with the incidence of colorectal cancer ,but it affects the prognosis of colorectal cancer in Han population.

2.
Chinese Journal of Cardiology ; (12): 143-146, 2010.
Article in Chinese | WPRIM | ID: wpr-341266

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the impact of statin use on coronary flow reserve (CFR) in patients with slow coronary flow.</p><p><b>METHODS</b>A total of 91 patients with chest pain and coronary slow flow but normal coronary angiography were included in this study, patients were divided into statin group (atorvastatin 20 mg/d for 8 weeks, n = 51) and non-statin group (n = 40), 26 healthy subjects with normal angiography and negative exercise ECG test served as normal controls. Blood cholesterol was measured. Doppler coronary flow velocity and Doppler reserve measurement of distal left anterior descending were recorded at rest and adenosine infusion (140 microgxkg(-1)xmin(-1)) induced hyperemia state, CFR was calculated by the ratio of maximal hyperemia and baseline peak diastolic coronary flow velocity (hCFV and bCFV) before and after atorvastatin treatment.</p><p><b>RESULTS</b>(1) Eight weeks later, total cholesterol and LDL-C levels were significantly lower in statin group than in non-statin group and control group [TC (3.83 +/- 0.80) mmol/L vs. (5.30 +/- 1.18) mmol/L vs. (5.32 +/- 1.17) mmol/L, P < 0.05; LDL-C (2.26 +/- 0.64) mmol/L vs. (3.28 +/- 0.85) mmol/L vs. (3.30 +/- 0.82) mmol/L, P < 0.05]. (2)Baseline CFR levels were significantly lower in statin group and non-statin group than that in control group (2.32 +/- 0.30 vs. 2.25 +/- 0.33 vs. 3.15 +/- 0.34, P < 0.05). Compared with non-statin group and statin group before treatment, 8 weeks statin treatment was associated with reduced bCFV [(26.06 +/- 3.22) cm/s vs. (29.02 +/- 3.36) cm/s and (26.06 +/- 3.22) cm/s vs. (28.43 +/- 3.40) cm/s, P < 0.05], increased hCFV [(77.63 +/- 8.96) cm/s vs. (65.17 +/- 7.22) cm/s and (77.63 +/- 8.96) cm/s vs. (64.58 +/- 6.26) cm/s, P < 0.05] and increased CFR (3.07 +/- 0.29 vs. 2.28 +/- 0.35 and 3.07 +/- 0.29 vs. 2.32 +/- 0.30, P < 0.05). bCFV, hCFV and CFR of statin group post treatment were similar to those of controls (P > 0.05).</p><p><b>CONCLUSION</b>Patients with coronary slow flow were associated with lower CFR which could be significantly improved by statin therapy.</p>


Subject(s)
Adult , Aged , Anticholesteremic Agents , Therapeutic Uses , Atorvastatin , Coronary Artery Disease , Drug Therapy , Female , Fractional Flow Reserve, Myocardial , Heptanoic Acids , Therapeutic Uses , Humans , Male , Middle Aged , Pyrroles , Therapeutic Uses
3.
Article in Chinese | WPRIM | ID: wpr-252082

ABSTRACT

<p><b>OBJECTIVE</b>In some hospitals an adult colonoscope is used for colon examination in children because they do not have child colonscope equipment. This has some disadvantages and this paper reports the experience for colon examination in children with an adult gastroscope instead of an adult colonoscope.</p><p><b>METHODS</b>One hundred and three children aged from 1.3 to 14 years who required routine colon examination were randomly assigned to adult gastroscope (n=49) and adult colonoscope groups (n=54).</p><p><b>RESULTS</b>There were no significant differences in the success rate of implantation into the ileocecum between the gastroscope and colonoscope groups (93.9% vs 94.4%; P>0.05). The average time of implantation into the ileocecum in the gastroscope group was shorter than that of the colonoscope group (5.2+/-1.1 min vs 7.3+/-2.9 min; P<0.05). Seventeen patients showed implantation-related complications in the colonoscope group but only 5 patients in the gastroscope group (P<0.01).</p><p><b>CONCLUSIONS</b>An adult gastroscope appears to be safer and more feasible than an adult colonoscope for colon examination in children.</p>


Subject(s)
Adolescent , Child , Child, Preschool , Colonoscopes , Colonoscopy , Methods , Female , Gastroscopes , Humans , Infant , Male
4.
Article in Chinese | WPRIM | ID: wpr-324058

ABSTRACT

<p><b>OBJECTIVE</b>To explore the operative approach and method of internal fixation for the treatment of type-C thoracolumbar fractures.</p><p><b>METHODS</b>T wenty-eight patients (male 20, female 8, ranging in age from 20 to 54 years, with an average of 38.5 years) with type-C thoracolumbar fractures were invovled in the study. Distributed segments involved T11 in 2 patients, T12 in 3 patients, L1 in 11 patients, L2 in 8 patient, L3 in 3 patients and L4 in 1 patient. Twenty-six patients were followed up (range from 12 to 20 months). According to the analysis of X-ray and CT image,height of vertebral body, Cobb angle on sagittal and coronal plane and the percentage of occupancy of vertebral canal were measured. The recovery of nerve, happening of back pain and the failure of internal fixation were observed.</p><p><b>RESULTS</b>The preoperative averaging height-loss decreased from 37.4% to 6.8% and the deformation of coronal plane was completely rectified. The preoperative averaging Cobb angle on sagittal plane recovered from 22.3 degrees to 5.6 degrees and the preoperative occupancy of vertebral canal averaging recovered from 33.7% to 5.9%. The difference was statistically significant (P < 0.05). Moreover, after 1 year follow-up, the changes of the above-mentioned index was no statistically significant (P > 0.05). Except for 8 patients with complete nerve damage losing the possibility of recovery, the others with incomplete nerve damage obtained 1 to 3 degree's improvement. The ratio of back pain occurrence was 19.2%. There was no failure of internal fixation.</p><p><b>CONCLUSION</b>The treatment of thoracolumbar type-C fractures with simple posterior long-segment internal fixation or posterior long-segmental fixation added by anterior autograft fusion is a reliable and effective method. The short-term therapeutic effect is satisfactory and the long-term therapeutic effect is to be further observed.</p>


Subject(s)
Adult , Female , Fracture Fixation, Internal , Methods , Humans , Lumbar Vertebrae , Wounds and Injuries , General Surgery , Male , Middle Aged , Radiography , Spinal Fractures , Diagnostic Imaging , General Surgery , Thoracic Vertebrae , Wounds and Injuries , General Surgery
5.
Chinese Journal of Cardiology ; (12): 615-619, 2007.
Article in Chinese | WPRIM | ID: wpr-307235

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effects of combined beta(1) adrenergic receptor (AR) antagonist with beta(2)AR agonist therapy on cardiac function and cardiomyocyte apoptosis in heart failure rats.</p><p><b>METHODS</b>Heart failure was induced by isoproterenol and rats were randomly divided into metoprolol group (50 mg/kg twice daily/gavage, n = 11), combined treatment group (fenoterol 125 microg/kg and metoprolol 50 mg/kg twice daily/gavage, n = 11) and placebo group (saline, n = 10), another normal 9 male Wistar rats served as control group. After 8 weeks' treatment, cardiac function, apoptosis index (AI), Caspase-3 activity, expression levels of bcl-2 and bax protein, organ weight/body weight and collagen volume fraction (CVF) were evaluated.</p><p><b>RESULTS</b>(1) Left ventricular end diastolic dimension, left ventricular end systolic dimension and E/A ratio were significantly increased and fractional shortening, ejection fraction significantly reduced post isoproterenol (all P < 0.05 vs. control) and these changes were significantly attenuated by metoprolol alone (all P < 0.05 vs. placebo) and further attenuated by the metoprolol and fenoterol combination therapy (all P < 0.05 vs. placebo and metoprolol). (2) Left ventricular weight to body weight ratio, lung weight to body weight ratio and CVF were also significantly reduced in metoprolol and combined treatment group than those in placebo group (all P < 0.01). (3) Compared with placebo group, AI and Caspase-3 activity were significantly lower in metoprolol group (all P < 0.01 vs. placebo) and further reduced in combined treatment group (all P < 0.01 vs. metoprolol). (4) The expression level of bax protein was significantly lower in metoprolol group while bcl-2/bax significantly higher than those in placebo group. These changes were more significant in combined treatment group (all P < 0.01 vs. metoprolol).</p><p><b>CONCLUSIONS</b>beta(1)AR antagonist in combination with beta(2)AR agonist further improved the cardiac function and prevented cardiac remodeling compared with using beta(1)AR antagonist alone in heart failure rats. Downregulated bax and upregulated bcl-2/bax expressions might contribute to the observed beneficial therapy effects by reducing cardiomyocyte apoptosis in these animals.</p>


Subject(s)
Adrenergic beta-1 Receptor Antagonists , Adrenergic beta-2 Receptor Agonists , Adrenergic beta-Agonists , Pharmacology , Therapeutic Uses , Adrenergic beta-Antagonists , Pharmacology , Therapeutic Uses , Animals , Apoptosis , Drug Therapy, Combination , Heart Failure , Drug Therapy , Male , Myocytes, Cardiac , Cell Biology , Rats , Rats, Wistar , Ventricular Remodeling
6.
Chinese Medical Journal ; (24): 2250-2255, 2007.
Article in English | WPRIM | ID: wpr-255803

ABSTRACT

<p><b>BACKGROUND</b>Stimulation of the heart beta 3-adrenoceptor (AR) may result in a negative inotropic effect. Being up-regulated, beta 3-AR plays a more important role in the regulation of cardiac function during heart failure. However, the effect of chronic blocking of beta 3-AR on heart failure has not been fully elucidated. In this study, we used a selective beta 3-AR antagonist SR59230A to treat a well defined heart failure rat model chronically, then evaluated its effect on cardiac function and investigated the mechanism.</p><p><b>METHODS</b>Male Wistar rats were chosen randomly as controls (n = 8). Isoproterenol induced heart failure rats were randomly divided into ISO group (n = 10) and SR group (n = 10). The ISO group received intraperitoneal injection of 1 ml saline twice a day; the SR group received intraperitoneal injection of SR59230A 85 nmol in 1 ml saline twice a day; and the control group received no treatment. The treatment was started 24 hours after the last isoproterenol injection and continued for 7 weeks. Then we measured the following indexes: the ratio of heart weight to body weight (HW/BW) and the ratio of left ventricular weight to body weight (LVW/BW), collagen volume fraction (CVF), left ventricular end diastolic dimension (LVEDd), left ventricular end systolic dimension (LVESd), ejection fraction (EF), fractional shortening (FS) and the ratio of E wave to A wave (E/A), the mRNA and protein expression of beta 3-AR and eNOS, and cGMP level in the heart.</p><p><b>RESULTS</b>The ratios HW/BW and LVW/BW were significantly increased in the ISO group compared with the control group (P < 0.01), but they were limited in the SR group (P < 0.05 compared with the ISO group). CVF increased in the ISO group and the SR group (P < 0.01), but it was significantly attenuated in the SR group (P < 0.01). LVEDd, LVESd and E/A ratio were significantly increased in the ISO group compared with the control group (P < 0.01), while EF and FS were significantly decreased (P < 0.01). Compared with the ISO group, the SR group showed that LVEDd, LVESd and E/A ratio were significantly decreased (P < 0.01), whereas EF and FS were significantly increased (P < 0.01). beta(3)-AR and eNOS mRNA and protein in the ISO group were significantly increased when compared with the control group (P < 0.01). These increases were all attenuated in the SR group compared with the ISO group (P < 0.01). The level of cGMP in myocardial tissue was significantly increased in the ISO group compared with the control group (P < 0.01), whereas SR59230A treatment normalized this increment (P < 0.01).</p><p><b>CONCLUSIONS</b>Chronic blocking of beta 3-AR could ameliorate cardiac function in heart failure rats and its mechanism involves inhibition of the negative inotropic effect and attenuation of cardiac remodeling.</p>


Subject(s)
Adrenergic beta-3 Receptor Antagonists , Adrenergic beta-Antagonists , Pharmacology , Therapeutic Uses , Animals , Blotting, Western , Disease Models, Animal , Echocardiography , Enzyme-Linked Immunosorbent Assay , Heart Failure , Drug Therapy , Male , Myocardium , Pathology , Nitric Oxide Synthase Type III , Genetics , Propanolamines , Pharmacology , Rats , Rats, Wistar , Receptors, Adrenergic, beta-3 , Physiology , Reverse Transcriptase Polymerase Chain Reaction , Ventricular Function, Left
7.
Article in Chinese | WPRIM | ID: wpr-346077

ABSTRACT

This study was aimed at assessing the effectiveness of Computer-assisted real-time diagnosis for micro-focus of esophagus. Two algorithms, the hues and saturation average of region-gradation (HSARG) and the wave-frequency integral of gradation (WFIG), based on the region-gradation change, were used to analyze the collected images of esophagus by quick, real-time processing. The results show that the image processing software based on the two algorithms mentioned above is effective in some degree for discriminating normal mucosa from the pathological changes of the esophagus such as esophagitis, early carcinoma of esophagus, middle-late carcinoma of esophagus, and atypical hyperplasia of esophagus. So the software may be used as a kind of auxiliary diagnostic measure to screen out the pathological changes of esophagus at clinic effectively.


Subject(s)
Algorithms , Endoscopy, Gastrointestinal , Esophageal Neoplasms , Pathology , Humans , Image Interpretation, Computer-Assisted
8.
Chinese Medical Journal ; (24): 1646-1652, 2006.
Article in English | WPRIM | ID: wpr-335554

ABSTRACT

<p><b>BACKGROUND</b>Experimental autoimmune myocarditis (EAM) in rats is a T-cell-mediated disorder. The initiation and maintenance of autoimmune responses in EAM depend on the maturation state of dendritic cells. IL-10 is a pleiotrophic immunomodulatory cytokine that functions at different levels of the immune response, so it has emerged as a promising therapeutic factor for the treatment of autoimmune/inflammatory diseases. This study was designed to test the hypothesis that IL-10 gene modified bone marrow-derived immature dendritic cells (iDCs) ameliorate EAM and to explore the underlying mechanisms.</p><p><b>METHODS</b>EAM was induced using the methods of cardiac myosin immunization on day 0 and day 7. Immature and mature bone marrow-derived dendritic cells (BMDCs) were generated without or with the stimulation by lipopolysaccharide (LPS) and the phenotype was analyzed by flow cytometry. Some of the iDCs were transfected by pcDNA3-IL-10 plasmid. 2 x 10(6)/per rat mature DC (mDC), immature DC (iDC), pcDNA3 transfected iDC, pcDNA3-IL-10 transfected iDC or phosphate buffered saline (PBS) were injected intravenously for treatment 5 days after the first immunization. On day 21, HE staining was performed to detect the myocardial inflammation and T lymphocyte proliferation assay was used to determine the effects of IL-10 gene transfected iDC on autoreactive T cell proliferation. Expression of IkappaB, the inhibitor of NF-kappaB pathway, was determined by Western blot.</p><p><b>RESULTS</b>BMDCs generated in a medium supplemented with granulocyte-macrophage-colony-stimulating factor (GM-CSF) were relatively immature, as determined by flow cytometry. However, stimulation with LPS induced these cells to become mature (m) DCs with higher levels of surface major histocompatibility complex (MHC)-II and costimulatory molecules. Intravenous administration of iDCs, especially pcDNA3-IL-10 transfected iDC, ameliorated the histopathological severity of the myosin induced-EAM, and the effect was lost after the DCs underwent maturation induced by in vitro exposure to LPS. IL-10 gene modified iDC inhibited the antigen specific T cell responses towards cardiac myosin. IkappaB protein was up-regulated significantly in the IL-10 gene modified iDC group.</p><p><b>CONCLUSIONS</b>IL-10 gene modified iDC induced antigen-specific tolerance in EAM. The underlying mechanisms may be related to costimulatory molecules down-regulation and NF-kappaB pathway inhibition.</p>


Subject(s)
Animals , Autoimmune Diseases , Allergy and Immunology , Dendritic Cells , Physiology , Immune Tolerance , Interleukin-10 , Genetics , Lymphocyte Activation , Male , Myocarditis , Allergy and Immunology , Myosins , Allergy and Immunology , NF-kappa B , Physiology , Rats , Rats, Inbred Lew , Signal Transduction , Transfection
9.
Chinese Journal of Cardiology ; (12): 703-707, 2006.
Article in Chinese | WPRIM | ID: wpr-238535

ABSTRACT

<p><b>OBJECTIVE</b>To investigate whether IL-10 gene modification on immature dendritic cells (iDC) could induce autoimmune tolerance in rat experimental autoimmune myocarditis (EAM).</p><p><b>METHODS</b>EAM was induced by cardiac myosin immunization on day 0 and day 7 in rats. A total of 2 x 10(6) mature DC (mDC), iDC, pcDNA3 transfected iDC, pcDNA3-IL-10 transfected iDC or PBS were injected intravenously at 5th immunization day. Three weeks later, echocardiography and HE staining were performed to observe the cardiac function and myocardial inflammation. Th1/Th2 cytokines were detected by ELISA and MHC-II molecules, costimulatory molecules were identified by flow cytometry. In vitro T lymphocyte proliferation assay and adoptive transfer of DCs were performed to determine the antigen specific tolerance induced by IL-10 gene modification on iDCs.</p><p><b>RESULTS</b>EAM rats treated with pcDNA3-IL-10 transfected iDC showed improved cardiac function and reduced inflammatory cells infiltration into myocardium. Moreover, lower Th1 and higher Th2-type response was induced, MHC-II and costimulatory molecules down-regulated and antigen specific immunological responses towards cardiac myosin inhibited in pcDNA3-IL-10-iDC treated EAM rats.</p><p><b>CONCLUSION</b>Treatment with IL-10 gene modified iDCs could ameliorates EAM by inducing Th2 polarization and down-regulation of MHC-II molecules and costimulatory molecule expressions.</p>


Subject(s)
Animals , Animals, Genetically Modified , Autoimmune Diseases , Allergy and Immunology , Bone Marrow Cells , Cell Line , Dendritic Cells , Allergy and Immunology , Genetic Therapy , Immune Tolerance , Interleukin-10 , Genetics , Allergy and Immunology , Myocarditis , Allergy and Immunology , Rats , Rats, Inbred Lew
10.
Chinese Journal of Cardiology ; (12): 69-72, 2005.
Article in Chinese | WPRIM | ID: wpr-243505

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the changes in the expressions of endothelial nitric oxide synthase (eNOS) and plasminogen activator inhibitor-1 (PAI-1) and the alterations of nitric oxide (NO) concentration in atrial endocardium in atrial fibrillation (AF) in order to investigate the mechanisms that contribute to thrombosis.</p><p><b>METHODS</b>In canine AF was produced with rapid atrial pacing at 400 bpm for 6 weeks, whereas the controls had no atrial pacing. NO production was measured by NO-specific microelectrode. The expression of endocardial eNOS and PAI-1 protein were determined by Western blot analysis and immunohistochemical Staining. Plasma levels of PAI-1 were analysed by Enzyme-linked immunoadsorbent assay.</p><p><b>RESULTS</b>Left atrial NO concentration was decreased in AF than that in controls [(23.4 +/- 5.8)nmol/L vs (63.8 +/- 16.1)nmol/L, P < 0.01]. Endocardial eNOS expression was also significantly decreased (855 +/- 217 vs 2320 +/- 694, P < 0.05), whereas the expression of the PAI-1 was increased (3164 +/- 827 vs 1371 +/- 352, P < 0.01). Neither NO concentration, nor PAI-1, eNOS expression were altered in the right atria at the same time. A significant increase for plasma levels of PAI-1 was also detected in AF group. No correlation was found between eNOS and PAI-1 protein expression (r = 0.217, P > 0.05).</p><p><b>CONCLUSION</b>In the canine model AF was associated with a marked decrease in endocardial NOS expression and NO concentration and with an increase in PAI-1 expression in the left atrium, which may contribute to the thrombosis in AF.</p>


Subject(s)
Animals , Atrial Fibrillation , Metabolism , Pathology , Disease Models, Animal , Dogs , Female , Male , Nitric Oxide , Metabolism , Nitric Oxide Synthase Type III , Metabolism , Plasminogen Activator Inhibitor 1 , Metabolism , Thrombosis , Metabolism , Pathology
11.
Chinese Journal of Hepatology ; (12): 90-92, 2002.
Article in Chinese | WPRIM | ID: wpr-334306

ABSTRACT

<p><b>OBJECTIVE</b>To explore the expression of integrin alpha 6 in hepatic sinusoidal capillaration.</p><p><b>METHODS</b>The rat hepatic fibrosis model was established by injection of carbon tetrachloride subcutaneously. Then the expression of laminin and integrin alpha 6 subunit was observed by immunohistochemistry and dot immuno-blotting.</p><p><b>RESULTS</b>We observed sinusoidal capillaration formed by deposition of laminin along sinusoids in Disse interspace by immunohistochemistry staining. In normal rat the expression of integrin alpha 6 was restricted to portal vascular endothelial cells and bile duct epithelial cell membranes. No expression was observed in sinusoidal endothelial cell membranes. When capillaration integrin alpha 6 was detected in a continuous pattern along the sinusoids, the content of integrin alpha 6 was significantly higher in fibrotic liver tissues than in normal liver tissues as measured by dot immuno-blotting (P<0.05).</p><p><b>CONCLUSIONS</b>During fibrogenesis, laminin continuously accumulate in liver tissues and form basement membrane resulting in sinusoidal capillaration, and then induce the expression of integrin alpha 6 on SEC membranes.</p>


Subject(s)
Animals , Antigens, CD , Carbon Tetrachloride , Immunoblotting , Immunohistochemistry , Integrin alpha6 , Laminin , Metabolism , Liver , Metabolism , Pathology , Liver Cirrhosis, Experimental , Metabolism , Pathology , Male , Rats , Rats, Wistar , Time Factors
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