Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add filters








Language
Year range
1.
Indian J Exp Biol ; 2015 Mar; 53(3): 177-183
Article in English | IMSEAR | ID: sea-158412

ABSTRACT

We studied the influence of sucrose and nitrogen concentration on in vitro flowering and fruit setting in elongated shoots of Withania somnifera. BA (1.5 mg/l) and IAA (0.3 mg/l) on MS medium supplemented with 4% sucrose showed 67% of in vitro flower induction frequency, 9 flowers/shoot, 4 fruits/shoot and 11 seeds/fruit in elongated-shoots. Different concentrations of nitrogen sources (L-glutamine, adenine sulphate, ammonium nitrate, potassium nitrate and sodium nitrate 5-25 mg/l) were tested in combination with 4% sucrose and BA at 1.5 mg/l and IAA at 0.3 mg/l. Highest number of flowers (20 flowers/shoot; 2.2-fold) and fruits (16 fruits/shoot; 3.39-fold), fruit setting (12 seeds/fruit; 1.08-fold) at a higher frequency (88 %) were achieved on MS medium augmented with 15 mg/l adenine sulphate with same PGRs and sucrose concentration. The maximum production of withanolide A (0.68 mg/g DW) and withanolide B (0.77 mg/g DW) was recorded in in vitro fruits. Highest accumulation of withaferin A (2 mg/g DW) was quantified from in vitro flowers, whereas, it was low in in vitro fruits (0.49 mg/g DW withaferin A). However, withanone (0.23 mg/g DW) was found accumulated uniformly in both in vitro flowers and fruits compared to control.


Subject(s)
Adenine/metabolism , Adenine/pharmacology , Carbon/metabolism , Culture Media/chemistry , Culture Media/pharmacology , Flowers/chemistry , Flowers/growth & development , Fruit/chemistry , Fruit/growth & development , Germination/drug effects , Glutamine/metabolism , Glutamine/pharmacology , Hydroponics , Nitrates/metabolism , Nitrates/pharmacology , Nitrogen/metabolism , Plant Shoots/chemistry , Plant Shoots/metabolism , Sucrose/metabolism , Sucrose/pharmacology , Withania/chemistry , Withania/growth & development , Withania/metabolism , Withanolides/metabolism
2.
Indian J Exp Biol ; 2013 Oct; 51(10): 849-859
Article in English | IMSEAR | ID: sea-149391

ABSTRACT

Majority of the Indian soybean cultivars are recalcitrant to tissue culture regeneration. The present communication reports the development of somatic embryogenesis in a liquid culture medium from immature cotyledons of G. max. Following induction with 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthalene acetic acid (NAA), the number of somatic embryos and percentage of explants that responded were higher with 45.24 µM 2,4-D. The proliferation of somatic embryos for three successive cycles was achieved in 22.62 µM 2,4-D. Histodifferentiation of somatic embryos under NAA (10.74 µM) indicated that better embryo development and maturation was achieved without any growth regulator. The amino acids such as L-glutamine favoured the somatic embryo induction and histodifferentiation at 20 and 30 mM respectively, where as L-asparagine at 10 mM concentration enhanced the somatic embryo proliferation. In addition, somatic embryos that were desiccated (air-drying method) for 5 days showed better germination (40.88%). The Indian soybean cultivars also showed strict genotypic influence and cv. Pusa 16 was emerged as a best responding cultivar for somatic embryo induction with 74.42% of response.


Subject(s)
Acclimatization/drug effects , Acclimatization/physiology , Amino Acids/pharmacology , Cell Differentiation/drug effects , Cell Proliferation/drug effects , Cotyledon/drug effects , Cotyledon/growth & development , Cotyledon/physiology , Desiccation , Germination/drug effects , Germination/physiology , Plant Growth Regulators/pharmacology , Plant Somatic Embryogenesis Techniques/methods , Glycine max/drug effects , Glycine max/growth & development , Glycine max/physiology
SELECTION OF CITATIONS
SEARCH DETAIL