Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add filters








Language
Year range
1.
Article in Chinese | WPRIM | ID: wpr-813962

ABSTRACT

OBJECTIVE@#To observe the inductive efficiency of deriving hematopoietic cells from human embryonic stem (hES) cells co-cultured with human yolk sac stromal cells, fetal liver stromal cells or fetal bone marrow stromal cells,in order to discuss the effect of the different hemopoietic microenvironment on hemopoietic cytogenesis.@*METHODS@#We used two-step method to induce the hES cells into the hematopoietic cells. In the first step the hES cells were co-cultured with cytokines by formation of the day 5 embryoid bodies (5d EBs). In the second step the 5d EB cells were induced into the hematopoietic cells by co-culturing with human yolk sac stromal cells, fetal liver stromal cells or fetal bone marrow stromal cells for 10 days. The inductive efficiencies of deriving hematopoietic cells from hES cells co-cultured with the different hemopoietic microenvironment were reflected by the expression levels of flk, CD34 and CD45 antigen.@*RESULTS@#Flow cytometry analysis demonstrated that the population of the cells co-cultured with human yolk sac stromal cells contained flk (1.80%+/-0.56%), CD34 (1.30%+/-0.14%) or CD45 (1.05%+/-0.63%) positive cells; the population of the cells co-cultured with human fetal liver stromal cells contained flk (34.00%+/-25.45%), CD34 (38.40%+/-24.80%) or CD45 (72.60%+/-25.70%) positive cells; the population of the cells co-cultured with human fetal bone marrow stromal cells contained flk (2.50%+/-1.48%), CD34 (3.20%+/-0.56%) or CD45 (1.65%+/-0.21%) positive cells. Compared with spontaneous differentiation of EBs, all of the three stromal cells could induce EBs into the hematopoietic cells (P<0.05).@*CONCLUSION@#The inductive efficiency of deriving hematopoietic cells from EBs co-cultured with human fetal liver stromal cells was higher than EBs co-cultured with human yolk sac stromal cells and fetal bone marrow stromal cells.


Subject(s)
Antigens, CD34 , Cell Differentiation , Cells, Cultured , Cellular Microenvironment , Coculture Techniques , Embryonic Stem Cells , Cell Biology , Fetus , Cell Biology , Hematopoietic Stem Cells , Cell Biology , Humans , Leukocyte Common Antigens , Mesenchymal Stem Cells , Cell Biology , Stromal Cells , Cell Biology , Yolk Sac , Cell Biology
2.
Article in Chinese | WPRIM | ID: wpr-282259

ABSTRACT

<p><b>OBJECTIVE</b>To study the yield of different Houttuynia cordata and the changing regularity of their main officinal components in different periods, and provide a basis for harvesting in good time and breeding new varieties.</p><p><b>METHOD</b>The main officinal composition and wet weight of overground parts per acreage of different accessions were determined periodically. The plot yields were tested when harvested and analyzed by analysis of variance.</p><p><b>RESULT</b>The difference of the yields of different accessions was significant. The varying tendency of the content of main officinal component methyl nonyl ketone and its total content per acreage of different accessions were different.</p><p><b>CONCLUSION</b>The integrate characters of Qionglai and Wangyu wild accessions are relatively better, worthy to be widely applied in production.</p>


Subject(s)
China , Drugs, Chinese Herbal , Ecosystem , Plant Components, Aerial , Chemistry , Plants, Medicinal , Chemistry , Saururaceae , Chemistry , Seasons , Species Specificity
SELECTION OF CITATIONS
SEARCH DETAIL