Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 22
Filter
1.
Chinese Journal of Endemiology ; (12): 128-132, 2024.
Article in Chinese | WPRIM | ID: wpr-1023997

ABSTRACT

Objective:To analyze the iodine nutritional status of children aged 8 - 10 in Tongren City, Guizhou Province, and provide a basis for scientific iodine supplementation for children.Methods:From 2020 to 2022, a systematic sampling method was adopted in 10 districts and counties of Tongren City. Each year, each district and county was divided into 5 districts based on east, west, south, north, and center. One township (street) was selected from each district, and 40 non boarding students aged 8 to 10 were selected from each township (street) to measure the iodine content of household salt and urine samples. The content of salt iodine in children of different yesas as well as the distribution of urine iodine in children of different districts and counties and different genders were analyzed and compared. Additionally, B-ultrasound was used to measure the thyroid volume of some children and the situation of thyroid enlargement was analyzed.Results:From 2020 to 2022, a total of 6 000 salt samples were collected and monitored from children's households, and 5 989 samples of iodized salt were detected, the coverage rate of iodized salt was 99.8%; and 5 750 samples of qualified iodized salt were found, the qualified rate of iodized salt was 96.0%, the consumption rate of qualified iodized salt was 95.8%; and the median salt iodine was 27.3 mg/kg, the difference in the median salt iodine among children between different years was statistically significant ( H = 10.04, P < 0.001). A total of 6 000 urine samples from children were tested, the median urinary iodine was 225.2 μg/L, the median urinary iodine among children in different districts and counties were statistically significantly different ( H = 85.73, P < 0.001); 3 077 male and 2 923 female urine samples were tested, and the median urinary iodine between different genders was statistically significant different ( Z = - 67.10, P < 0.001). The median urinary iodine of male samples were higher than those of female samples(227.8 vs 222.9 μg/L). The thyroid gland of 2 000 children was examined, and the rate of goiter was 1.0% (21/2 000). Conclusions:From 2020 to 2022, the consumption rate of qualified iodized salt, urinary iodine content and goiter rate of children in Tongren City have all met the national standard for eliminating iodine deficiency disorders. The overall iodine nutrition level exceeds the appropriate amount (urinary iodine of 200 - 299 μg/L).

2.
Chinese Journal of Microsurgery ; (6): 310-314, 2022.
Article in Chinese | WPRIM | ID: wpr-958372

ABSTRACT

Objective:To observe the effects of polishing the anterior and posterior capsule with irrigation/aspiration (I/A) injection needle and capsular tension ring (CTR) implantation on intraocular lens (IOL) capsular stability after phacoemulsification for ultra-high myopia with 2.0 mm coaxial micro incision.Methods:This is a prospective randomized controlled study. There were 40 patients(80 eyes) aged 46-72 years old with ultra-high myopia cataract. The grade of lens opacity was grade II-IV, and the diopter was -10 D - -24 D. During 2.0 mm coaxial micro incision phacoemulsification, adopt coin tossing method randomly, 1 eye was operated using the anterior and posterior capsule polishing 360° with I/A injection needle combined with CTR implantation as the experimental group(40 eyes), the other eye was neither polished nor CTR implantated as the control group(40 eyes). The operation interval of both eyes was less than 1 week. The size of anterior capsular orifice, effective intraocular lens position(ELP), IOL eccentricity and posterior capsular opacification(PCO) were recorded at 1 week, 1, 3 and 6 months after operation in outpatient clinic. Two independent sample t-test and Fisher exact probability test were used to compare the differences between the 2 groups. P<0.05 was defined significant difference. Results:During the follow-up period, there were no significant change in the anterior capsule area, IOL eccentricity and ELP in the experimental group. However in the control group, the anterior capsular area decreased gradually with time, and gradually IOL eccentricity increased and ELP decreased. There was no significant difference between the 2 groups in each observation index at 1 week after operation, but it was ELP that first showed the difference tendency( P<0.01). The ELP of the control group was significantly lower than that of the experimental group at 1, 3 and 6 months after operation( P<0.05). There was no significant difference in anterior capsule area and IOL eccentricity between the 2 groups at 1 and 3 months after operation( P>0.05), but there was a significant difference at 6 months( P<0.05). In terms of PCO, the incidence of PCO was 0%, 2.5% and 7.5% in the experimental group and 5.0%, 17.5% and 32.5% in the control group respectively at 1, 3 and 6 months after operation. The incidence of PCO in the experimental group was significantly lower than that in the control group( P<0.05). Conclusion:Ultra-high myopia with cataract are prone to have capsular contraction after operation, which is characterized by IOL instability and anterior capsular orifice narrowing gradually. Combined 360° anterior and posterior capsular polishing with tension ring implantation in operation can effectively maintain the stability of ELP, reduce the degree of contraction of anterior capsular orifice, reduce the eccentricity of IOL and the incidence of PCO. Polishing the anterior and posterior capsule with I/A injection needle combined with CTR implantation, is safe and effective for patients with ultra-high myopia cataract.

3.
Article in Chinese | WPRIM | ID: wpr-1015831

ABSTRACT

In this study, we performed high-throughput sequencing technology methylated RNA immunoprecipitation sequencing (MeRIP-seq), transcriptome sequencing (RNA-seq) and bioinformatics to analyze the differentially m6A-methylated and differentially expressed profile of circular RNA (circRNA) in middle cerebral artery occlusion/reperfusion (MCAO/R) model, which provided some scientific evidences for revealing the relationship between RNA epigenetic modification and cerebral ischemia reperfusion injury. The neurological deficit scores of mice were evaluated by the Longa score standard. TTC staining was used to detect cerebral infarction volumes, and dot blot was used for the quantification of m

4.
Article in Chinese | WPRIM | ID: wpr-905241

ABSTRACT

Objective:To explore the effects of enriched environment on neurological function in cerebral ischemia-reperfusion injury rats and the glucose metabolism in ischemic penumbra. Methods:A total of 72 adult male Sprague-Dawley rats were randomly divided into sham group (n = 24), model group (n = 24) and enriched environment group (n = 24). The latter two groups suffered cerebral ischemia 60 minutes and reperfused with modified Longa's method. The enriched environment group was fed in enriched environment after operation. All the rats were assessed with modified Neurological Severity Score (mNSS) before, and one, seven, 14, 21 and 28 days after operation. One and 28 days after operation, twelve rats from each groups were sacrificed after mNSS assessment, respectively. The histopathology was observed with HE staining. The expressions of hypoxia-inducible factor-1α (HIF-1α), glucose transporter 1 (GLUT1) and 6-phosphofructo-2-kinase/fructose-2-bisphosphatase 3 (PFKFB3) in ischemic penumbra were determined with reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) and Western blotting. The levels of ATP, ADP and AMP in ischemic penumbra were measured with high performance liquid chromatography (HPLC) and the energy charge (EC) was calculated. Results:Compared with the model group, the scores of mNSS decreased in the enriched environment group since 14 days after operation (P < 0.05). The cells in the penumbra presented edema, nuclear pyknosis marginalization, vacuolar arrangement and other pathological changes in the model group and the riched environment group one day after operation; while compared with the model group, the levels of ATP and EC decreased and the mRNAs and protein expression of HIF-1α, GLUT1 and PFKFB3 increased in the enriched environment group (P < 0.05). The pathology improved in the riched environment group compared with that in the model group 28 days after operation; while the mRNAs and protein expression of HIF-1α, GLUT1 and PFKFB3 increased, as well as the levels of ATP and EC (P < 0.05). Conclusion:Enriched environment can promote the recovery of neurological function in rats after cerebral ischemia-reperfusion, which may associate with promoting expression of HIF-1α and downstream GLUT1 and PFKFB3, and improving glucose metabolism.

5.
Yao Xue Xue Bao ; (12): 414-431, 2021.
Article in Chinese | WPRIM | ID: wpr-873760

ABSTRACT

Protein kinases are intimately involved in the pathogenesis of many diseases such as cancer, inflammation, and autoimmune and neurological diseases. Therefore, kinases have been widely studied as drug targets over the past three decades. As of April, 2020, the FDA had approved 59 small molecule kinase inhibitors (SMKIs) in the emerging field of targeted drug therapy. This paper focuses on the biochemistry and pharmacology of these 59 SMKIs and 121 SMKIs for which structures can be retrieved and that are now in phase Ⅱ and Ⅲ clinical trials. In addition, this paper also conducts a simple analysis of several popular targets and their inhibitors.

6.
Chinese Journal of Rheumatology ; (12): 543-547, 2019.
Article in Chinese | WPRIM | ID: wpr-791345

ABSTRACT

Objective This study aims to investigate the variation of neutrophil extracellular traps (NETs) in animal model of staphylococcus aureus (SA) infected systemic lupus erythematosus (SLE) MRL/Lpr mouse.Methods The infection model was developed by injecting the Staphylococcus Aureus in the mouse tail vein.The Staphylococcus Aureus load of the serum and kidney was detected by enzyme-linked immuno sorbent assay (ELISA).The level of NETs complex in the serum was detected by Cell-Death-Elisa-Plus-Kit and anti-myeloperoxidase (MPO) antibody.The kidney NETs formation was tested with the immunofluorescence.Statistical program for social sciences (SPSS) 17.0 software and Image Pro Plus statistical software were used for analysis.Comparisons between groups were made using unpaired t test for normally distributed numerical data,nonparametric test for non-normally distributed numerical data,and single factor analysis (ANOVA) for variance.Results The incidence of walking instability,purulent exudate in eyes and death was 2/6,1/6,1/6 respectively in Staphylococcus Aureus-infected MRL/Lpr mice,However,The control group had no death and related symptoms.The Staphylococcus Aureus load of the serum was higher in MRl/lpr mice when compared to the control group [(106.79±23.39) ng/ml vs (48.82±11.49) ng/ml,U=2.739,P<0.05] after Staphylococcus Aureus infection.The control group had a higher NETs level [(2.24±0.15) vs (1.64±0.08),U=2.882,P<0.05],however,all the MRL/Lpr mice had no significant change of the NETs level [(2.63±0.61) vs (2.65 ±0.260),U=0.548,P>0.05] after Staphylococcus Aureus infection.Conclusion After infected by the Staphylococcus Aureus,more symptoms,higher death rate and higher Staphylococcus Aureus load of the serum present in the MRlL/lpr mice than the control group.While,there's none significant change of the NETs level in Staphylococcus Aureus-infected MRLL/lpr mice.This suggests that the dyspoiesis of NETs is present in Staphylococcus Aureus-infected MRL/lpr mice.

7.
Chinese Journal of Immunology ; (12): 431-435, 2018.
Article in Chinese | WPRIM | ID: wpr-702748

ABSTRACT

Objective:To investigate the value of autoantibodies and serum levels of IgG4 and CA19-9 in the diagnosis of IgG4 associated cholangitis (IgG4-SC).Methods:Detect the serum IgG4 and CA19-9 of 41 clinical cases of IgG4-SC patients,162 clinical cases of non IgG4-SC patients and 40 healthy human serum samples by immunoassay and direct chemiluminescence methods, also detect the antinuclear antibodies (ANA),anti neutrophil antibody (ANCA),anti smooth muscle antibody (SMA) and anti mitochondrial antibody (AMA) of the above serum samples by indirect immunofluorescence and analyze the detection results.Results:①The positive rates of ANA,ANCA,SMA and AMA in patients with IgG4-SC were 41.46%,7.32%,0 and 2.44%.Among them,the positive rate of ANA was significantly different from that of the normal control group(P<0.01),and the positive rate of SMA and AMA was significantly different from that of non IgG4-SC group(P<0.01),and so as the positive rate of ANCA do with that of PSC group.②The number of serum IgG4 and CA19-9 increased samples were significantly compared with the normal control group (P<0.01);the area under the ROC curve (AUC) was 0.979 and 0.646,respectively,and P<0.05.Conclusion:The high level of serum IgG4 and CA19-9 and autoantibody detection are of great accuracy and important clinical value in the differential diagnosis of IgG4-SC.

8.
Chin. med. j ; Chin. med. j;(24): 420-427, 2017.
Article in English | WPRIM | ID: wpr-303136

ABSTRACT

<p><b>BACKGROUND</b>The treatment of the condylar fractures is difficult. Factors that result in the fractures are complex. The objective of this morphometric study was to investigate the relationship between condylar fracture patterns and condylar morphological characteristics.</p><p><b>METHODS</b>We conducted a retrospective analysis of 107 patients admitted to the West China Hospital of Stomatology for bilateral condylar fractures caused by parasymphyseal impact. The patients were divided into five groups according to the type of condylar fracture. Ten parameters were evaluated on three-dimensional (3D) reconstruction mandible models through the Mimics 16.0 (Materialize Leuven, Belgium) anthropometry toolkit. Each parameter of the 3D models was analyzed using multivariate analysis. Multinomial logistic regression analyses were used to examine the relationships between the five groups.</p><p><b>RESULTS</b>The results showed that the differences of condylar head width (M1), condylar neck width (M3), the ratio of condylar head width to condylar anteroposterior diameter (M1/M2), the ratio of condylar head width to condylar neck width (M1/M3), the ratio of condylar height to ramus height (M8/M7), and mandibular angle (M10) were statistically significant (p < 0.05). Type A condylar head fractures were positively associated with M1 (compared to Type B: OR =1.627, 95% CI: 1.123, 2.359; compared to Type C: OR = 1.705, 95% CI: 1.170, 2.484) and M1/M2 (compared to Type B: OR =1.034, 95% CI: 0.879, 2.484). Type B condylar head fractures were negatively associated with M10 (compared to Type C: OR = 0.909, 95% CI: 0.821, 1.007). Condylar neck fractures were negatively associated with M3 (compared to condylar head: OR = 0.382, CI: 0.203, 0.720 ; compared to condylar base: OR = 0.436, 95% CI: 0.218, 0.874), and positively associated with M1/M3 (compared to condylar head: OR = 1.229, 95% CI: 1.063, 1.420 compared to condylar base: OR = 1.223, 95% CI: 1.034, 1.447). Condylar base fractures were positively associated with M10 (OR = 1.095, 95% CI: 1.008, 1.189) and negatively associated with M8/M7 (OR = 0.855, 95% CI: 0.763, 0.959) as compared with condylar head fractures.</p><p><b>CONCLUSIONS</b>Condylar fracture pattern is associated with the anatomical features of the condyles when a fracture occurs from parasymphyseal impact.</p>


Subject(s)
Adult , Female , Humans , Male , Middle Aged , Young Adult , Fractures, Bone , General Surgery , Mandibular Condyle , Wounds and Injuries , General Surgery , Mandibular Fractures , General Surgery , Multivariate Analysis , Retrospective Studies
9.
Journal of Chinese Physician ; (12): 1810-1812,1816, 2017.
Article in Chinese | WPRIM | ID: wpr-705751

ABSTRACT

Objective To explore the efficacy of biomimetic physiotherapy in preventing adhesions and promote endometrial repair and growth after Transcervical resection of adhesions ( TCRA) . Methods A prospective case-control study method, from January 2016 to May 2017 in Hunan Maternal and Child Health Hospital underwent TCRA in 92 patients were randomly divided into study (45 cases) and control (47 cases) groups. The patients in the study group were treated with biomimetic therapy after TCRA, and were treated with artificial cycles. Three menstrual cycles were treated continuously. The control group was treated with hormone artificial cycle. The menstrual recovery, ovulation endometrium thickness, uterine ar-tery blood flow resistance index ( RI) and second-look hysteroscopy were observed and compared between two groups before and after 1 course of treatment. Results After 1 course of treatment, the efficiency of the study group was significantly better than that of the control group (P<0. 05), and the cure rate of the study group was significantly higher than that of the control group ( P<0. 05 ) . The endometrial thickness of the study group were increased significantly after ovulation (P<0. 05). The uterine artery blood flow resistance was decreased significantly after treatment in the study group (P<0. 05). Conclusions Bionic physiother-apy can improve pelvic circulation, promote endometrial growth, and prevent the formation of adhesions after TCRA to a certain extent.

10.
Recent Advances in Ophthalmology ; (6): 140-142,146, 2017.
Article in Chinese | WPRIM | ID: wpr-606346

ABSTRACT

Objective To compare the efficacy and safety of vitrectomy combined with internal limiting membrane flap and vitrectomy combined with internal limiting membrane peeling for the treatment of idiopathic macular hole with different sizes.Methods A total of 127 consective patients (127 eyes)were divided into two groups according to the size of the hole diameter of the smallest split points by less than or equal to 500 μm (small diameter macular hole group) and more than 500 μm (huge diameter macular hole group).According to different surgical methods the patients were divided into non ILM flap coverage group (peeling 1 group and peeling 2 group) and ILM flap cover group (covering 1 group and covering 2 group).All the patients underwent vitrectomy combined with internal limiting membrane peeling or vitrectomy combined with limiting membrane flap.Preoperative and postoperative best correct visual acuity,closure ratio of macular hole and postoperative major complications were observed and followed up.Results The postoperative best correct visual acuity improved in all the groups,there was no significance difference between small diameter macular hole group and huge diameter macuiar hole group (t =0.112 2,0.750 8;all P >0.05).The closure ratio of peeling 1 group and covering 2 group at postoperative 6 months were all 100%,there was no statistical difference (P > 0.05),which in peeling 2 group and covering 2 group were 84.85% and 100.00%,there was statistical difference (x2 =13.292,P < 0.05).There was no statistical difference in preoperative defect diameter of the inner and outer junction between peeling 2 group and covering 2 groups (P >0.05),there was also no statistical difference between peeling 2 group and covering 2 groups at postoperative 1 months (P > 0.05),but there were statistical differences at postoperative 3 months,6 months and 12 months (all P < 0.05),the covering 2 group were less than the peeling 2 group.Conclusion ILM flap coverage helps to heal macular holes greater than 500 μm diameter,and has no extra effect on healing of diameter less than 500 μm.

11.
Tianjin Medical Journal ; (12): 994-997, 2017.
Article in Chinese | WPRIM | ID: wpr-610789

ABSTRACT

Objective To understand the status of comprehensive health function impairment in elderly patients with rheumatoid arthritis, and to explore its influencing factors. Methods A total of 80 elderly patients with rheumatoid arthritis were enrolled in the first Affiliated Hospital of Kunming Medical University from December 2014 to October 2016. The quality of life SF-36 score was used to measure the physical health, mental health, daily activities and social health to assess the overall health status of patients. The factors influencing the comprehensive health function of the patients were analyzed by univariate analysis and multivariate linear regression analysis. Results The univariate and multivariate analysis showed that the low level of education, general occupation, marital misfortune, poor economic condition and self-treatment expense were the risk factors affecting the comprehensive health function in elderly patients with rheumatoid arthritis ( P<0.05). Conclusion Elderly patients with rheumatoid arthritis have problems of the functions of daily life, physical health, mental health and social health. To alleviate the high medical costs, improve the level of medical treatment, improve the people 's health and education level and maintain a good mood are the key to improve the overall health in patients with rheumatoid arthritis.

12.
Recent Advances in Ophthalmology ; (6): 1182-1184, 2017.
Article in Chinese | WPRIM | ID: wpr-669078

ABSTRACT

Objective To investigate the efficacy of ranibizumab combined with pars plana vitrectomy (PPV) in treatment of patients with diabetic retinopathy together with neovascular glaucoma.Methods A retrospective analysis of 16 patients (16 eyes) with diabetic retinopathy accompanied by neovascular glaucoma underwent vitrectomy combined with ranibizumab between June 2013 and June 2017 was conducted.Data were collected on patients' intraocular pressure (IOP),best-corrected visual acuity (BCVA),iris neovascularization,chamber angle changes before and after surgery,and the postoperative complications were recorded.Results No significant difference was approached in IOP before surgery and 1 day after ranibizumab injection [(42.54 ± 16.52) mmHg vs.(38.78 ± 11.38)mmHg,1 kPa =7.5 mmHg] (P>0.05).And there was also no significant difference in the IOP before surgery and 1 week after vitrectomy [(45.30 ± 12.20) mmHg vs.(42.54 ± 16.52) mmHg] (P >0.05);The IOP at 1 month,3 months and 6 months after surgery was (27.56 ± 19.38) mmHg,(20.49 ± 18.66) mmHg and (19.79 ± 16.53) mmHg,respectively,which were significantly different from preoperative IOP (all P < 0.05).At the end of the follow-up,iris neovascularization completely disappeared in 10 eyes (62.5%) and iris neovascularization was significantly lessened in 6 eyes (37.5%).Conclusion Ranibizumab with PPV for diabetic retinopathy and neovascular glaucoma can effectively reduce intraocular pressure and postoperative complications as well as improve visual acuity slightly.

13.
Article in Chinese | WPRIM | ID: wpr-493944

ABSTRACT

Objective The aim of this study was to investigate the distribution of body weight related index and to explore the relationship between weight related index and cardiovascular diseases in rural residents in Yunnan Province. Methods By random cluster sampling method,rural residents were selected in Yunnan Province. The data were collected by face-to-face questionnaire and medical examination. Results 4070 rural residents in Yunnan participated in this study. The survey results showed that gender,age,education level and ethnicity affected both the distribution of body weight related index and low body weight,overweight,obesity,and central obesity(P < 0.05). Compared with low body weight,non overweight,non obesity and non central obesity group,non low body weight,overweight,obesity and central obesity group had higher prevalence rate of hypertension(P < 0.01). Among central obesity group,the prevalence rate of coronary heart disease was higher than non central obesity group(P < 0.01). Conclusion The distribution of body weight related index in rural residents of Yunnan province showed some special characteristic. The overweight,obesity,and central obesity were related to cardiovascular diseases. Change unhealthy living habits or behavior as well as improving their cultural quality have effects on controlling body weight related index and help to reduce the prevalence rates of overweight,obesity and central obesity which can further reduce the prevalence rates of cardiovascular diseases.

14.
Journal of Chinese Physician ; (12): 868-871,875, 2015.
Article in Chinese | WPRIM | ID: wpr-601544

ABSTRACT

Objective To explore the rehabilitation effect of pelvic floor muscle training combined with traditional Chinese medicine in the treatment of mild-moderate pelvic organ prolapse.Methods A prospective ease-control study,choosing 103 patients of mild-moderate pelvic organ prolapse from October 2012 to May 2014 in Maternal and Child Health Hospital Outpatient of Hunan Province,which were randomly divided into study group (52 cases) and control group (51 cases).All of the patients underwent two courses of pelvic floor muscle training,including Kegel exercise,biofeedback,electrical stimulation therapy,the study group combined with traditional Chinese medicine (Buzhongyiqitang) at the same time.The efficacy was analyzed before and after treatment of pelvic floor muscle strength,myoelectric potential and indexing of pelvic organ prolapse quantification (POP-Q) changes.Results There werent statistically significant differences in type Ⅰ and Ⅲ muscle fiber muscle strength and myoelectric potential of two groups before treatment,while the shrinkage index improved significantly after treatment,and the study group was significantly higher with statistically significant difference (P < 0.05).The effective rates of POP-Q indexing changes in two groups were 71.2% and 56.9%,respectively.The effective rate of the study group was significantly higher than the control group(P < 0.05).Conclusions The clinical efficacy of pelvic floor muscle training combined with Buzhongyiqitang in the treatment of mild-moderate pelvic organ prolapse was significant,and it had a good clinical value.

15.
Cancer Research and Clinic ; (6): 708-710, 2013.
Article in Chinese | WPRIM | ID: wpr-441663

ABSTRACT

Cancer is one of the most dangerous diseases that threaten human health.The early diagnosis individual treatment and accurate judgement of recurrence play important roles in prognosis.microRNA (miRNA) is a class of small fragments of RNA molecules which has the ability to regulate the gene expression.In recent years,some researchers have begun to focus on it.Studies have shown that the great stability of circulating miRNA in body fluid,as well as the specificity in many types of cancer,which may be important for the diagnosis and prognosis of cancer patients.In this review,the advantages and limitations of circulating miRNA as a new biomarker of cancer are birfly summarized.

16.
Chinese Journal of Virology ; (6): 652-657, 2012.
Article in Chinese | WPRIM | ID: wpr-339990

ABSTRACT

To investigate the effects of DENV infection on the expression of Vascular endothelial growth factor (VEGF) in monocytes, and to explore which innate immune signaling pathway is responsible for VEGF induction. Real-time PCR was used to determine the expression levels of VEGF in DENV-infected THP-1. We found that different serotype viruses (DENV1, DENV2, DENV3) induced the VEGF expression. Moreover, VEGF expression was significantly increased in human primary monocytes infected with DENV 2. In addition, VEGF induction by DENV2 was significantly impaired by knockdown of TLR3 and interferon-beta promoter stimulator 1 (IPS-1), or by inhibition of ERK, JNK or NF-kappaB. These results demonstrated that DENV induced VEGF expression in monocytes, and the activation of TLR3, IPS-1 signal pathways were required for DENV2-triggered VEGF induction, suggesting that VEGF might be a promising therapeutic target for DHF.


Subject(s)
Humans , Dengue , Genetics , Allergy and Immunology , Virology , Dengue Virus , Classification , Genetics , Physiology , MAP Kinase Signaling System , Monocytes , NF-kappa B , Genetics , Allergy and Immunology , Up-Regulation , Vascular Endothelial Growth Factor A , Genetics , Allergy and Immunology
17.
Article in Chinese | WPRIM | ID: wpr-336083

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effect of pBBADs-OXM-transformed bifidobacteria on the body weight of obese mice.</p><p><b>METHODS</b>B. longum was transformed with pBBADs-OXM by electroporation, and arabopyranose-induced oxyntomodulin expression by the bacterium was detected by ELISA. pBBADs-OXM-transformed bifidobacteria was administered orally obese mice on a daily basis with pBBADs-GFP-transformed bifidobacteria as the negative control, and the body weight changes of the mice were observed.</p><p><b>RESULTS</b>OXM was detected by ELISA not only in the supernatant but also the precipitant of the transformed bacterial culture. The body weight of the obese mice fed with pBBADs-OXM-transformed bifidobacteria decreased significantly compared with that of the mice in the obese model group (P<0.05).</p><p><b>CONCLUSION</b>Administration of pBBADs-OXM-transformed B.longum can reduce the body weight of obese mice.</p>


Subject(s)
Animals , Mice , Administration, Oral , Appetite Depressants , Metabolism , Bifidobacterium , Genetics , Metabolism , Body Weight , Electroporation , Escherichia coli , Genetics , Metabolism , Obesity , Drug Therapy , Oxyntomodulin , Genetics , Random Allocation , Recombinant Proteins , Genetics
18.
Zhonghua nankexue ; Zhonghua nankexue;(12): 779-785, 2008.
Article in Chinese | WPRIM | ID: wpr-309795

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects of prepubertal continuous exposure to dibutyl phthalate (DBP) on the testis development in SD rats.</p><p><b>METHODS</b>Twenty-one-day-old weanling prepubertal male SD rats were randomly divided into a control (n = 24) and an experiment group (n = 54), gavaged daily with corn oil vehicle or corn oil + DBP at the repeated dose of 0 mg/(kg x d) (control), 50 mg/(kg x d) (low-dose), 200 mg/(kg x d) (medium-dose) and 600 mg/(kg x d) (high-dose) for 14, 21 and 28 days, and then sacrificed by decapitation on PND35, PND42 and PND49. The body weight gain, the testis weight and volume and the weight of accessory sex organs were measured, the serum testosterone level assayed by chemoluminescence technique, the testis tissues stained by H&E and observed under the light microscope for morphological alteration, the mean diameter of the seminiferous tubules determined and testicular biopsy scores obtained.</p><p><b>RESULTS</b>Disordered arrangement of spermatogenic cells was found in some seminiferous tubules on PND35 in the low-dose group, but testis development and spermatogenesis were normal on PND42 and PND49. In the medium-dose group, disordered arrangement and decreased number of spermatogenic cells were observed on PND35 and PND42, but without testicular atrophy, and various grades of spermatogenic cells and sperm were seen on PND49. High-dose DBP slowed down the body weight gain, decreased serum T levels and induced degeneration of seminiferous tubules, arrest of spermatogenic epithelium development and necrosis of spermatogenic cells. The pubertal rats (PND49) showed testicular atrophy, azoospermia and delayed development of accessory sex organs.</p><p><b>CONCLUSION</b>Prepubertal continuous exposure to DBP induces damages to testicular development and spermatogenesis in a dose-dependent manner, and those induced by high-dose DBP cannot be recuperated in the phase of prepubertal development, while the slight adverse effects on the testis induced by low- and medium-dose DBP could be completely or partly reversible before PND49.</p>


Subject(s)
Animals , Male , Rats , Dibutyl Phthalate , Toxicity , Environmental Exposure , Growth , Rats, Sprague-Dawley , Testis
19.
Zhonghua Wai Ke Za Zhi ; (12): 1237-1241, 2007.
Article in Chinese | WPRIM | ID: wpr-340822

ABSTRACT

<p><b>OBJECTIVE</b>To discuss the failure reasons of operation for spinal tuberculosis complicated with paraplegia and methods of the second operation.</p><p><b>METHODS</b>Spinal tuberculosis paraplegic patients (18 males, 14 females) were reviewed retrospectively. They have been treated with failing decompressive surgery from January 2001 to December 2006. Seventeen patients received anterior debridement surgery via transpleural approach while the other 15 patients received posterolateral decompression surgery via costotransverse approach. Twenty-two patients got chemotherapy after the surgery.</p><p><b>RESULTS</b>Twenty-three patients were treated by anterior debridement, decompression and graft placement via transpleural approach (9 received the single-stage posterior instrumentation). Five patients received posterolateral debridement and decompression via extrapleural approach. Two patients, recur focus be eliminated. Two patients were given sinus debridement surgery alone. All patients were given anti-tuberculosis chemotherapy. The paraplegia was recovered completely in 26 patients, and partly in 5 patients.</p><p><b>CONCLUSIONS</b>Inadequate treatment results in defeated operative. The proper selection of operative modalities and timing on the basis of systematically anti-tuberculosis chemotherapy remains the best mode of therapy for spinal tuberculosis complicated with paraplegia. And it is also essential to choose a radical debridement surgery to decompress the spinal cord and to reconstruct the stability of spine.</p>


Subject(s)
Adolescent , Adult , Aged , Child , Female , Humans , Male , Middle Aged , Follow-Up Studies , Paraplegia , Reoperation , Retrospective Studies , Treatment Failure , Tuberculosis, Spinal , Drug Therapy , General Surgery
20.
Article in Chinese | WPRIM | ID: wpr-684889

ABSTRACT

Human parathyroid hormone peptide1-34(hPTH1-34) was highly expressed in Escherichia coli by inserting the synthesized hPTH1-34 cDNA into pThioHis, the prokaryotic expression vector. The expressed hPTH1-34 was purified by chelating sepharose immobilized metal ion affinity, reverse and filter chromatographic steps. Its purity was verified above 95% by HPLC. The quality was identified by N-terminal sequencing and MALDI-TOF-MS analysis. In vitro analysis showed the adenylate cyclase of ROS 17/2.8 cells was activated by hPTH1-34.

SELECTION OF CITATIONS
SEARCH DETAIL