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1.
Article in Chinese | WPRIM | ID: wpr-905294

ABSTRACT

Walking is the embodiment of the ultimate goal of the central nervous system at the level of biomechanics, which is of great significance to the formation of children's psychological and motor independence. It is necessary to improve walking mobile function to obtain independent mobile ability and the quality of daily life. The standard of routine walking training cannot be unified, and the evaluation index cannot be standardized and quantified. Robot-assisted lower limb rehabilitation is the research hotspot in the field of improving walking mobile function in neurological dysfunction in recent years, which is characterized by high-intensity and repetitive locomotion. This paper briefly introduced the development, classification and rehabilitation mechanism of robot-assisted lower limb rehabilitation, and summarized the application of robot-assisted gait training in the rehabilitation of walking mobile dysfunction of children with spastic cerebral palsy based on the framework of International Classification of Functioning, Disability and Health. Robot-assisted lower limb rehabilitation can significantly improve the range of motion, muscle strength and spatiotemporal parameters of spastic cerebral palsy, and significantly improve the short-term motor and balance function. However, the researches on reducing abnormal muscle tone, reducing energy expenditure and improving participation ability are still controversial, and the standard of long-term efficacy and intervention measures still needs to be further studied. In the future, it is necessary to carry out multi-center research with large samples, so that the robot-assisted lower limb rehabilitation can be used as a supplementary mean of routine physical therapy for children with spastic cerebral palsy.

2.
Article in Chinese | WPRIM | ID: wpr-905137

ABSTRACT

Objective:To investigate the effect of robotic-assisted gait training on the motor function and walking ability for children with spastic cerebral palsy. Methods:From October, 2020 to April, 2021, 34 children with spastic cerebral palsy were randomly divided into control group (n = 17) and experimental group (n = 17). The control group received conventional physical therapy 60 minutes a day, while the experimental group received physical therapy and robotic-assisted gait training, 30 minutes a day respectively. They were assessed with 10-meter walk test (10MWT), 6-minute walk distance (6MWD), Physiological Consumption Index, Gross Motor Functional Measure (D and E dimensions), center of pressure envelope area, and Functional Ambulation Categories before training, eight weeks after training, and one-month follow-up. Results:Four cases dropped out. All the indexes improved in the experimental group after training (|t| > 3.219, P < 0.05), and they were stable as follow-up (|t| < 1.565, P > 0.05), except 10MWT (|t| > 3.491, P < 0.01); while they were better than those in the control group (|t| > 2.108, P < 0.05), except 6MWD. Conclusion:Robotic-assisted gait training can promote the recovery of motor, balance and walking for children with spastic cerebral palsy based on conventional physical therapy, and remain at least one month.

3.
Journal of Experimental Hematology ; (6): 1318-1324, 2021.
Article in Chinese | WPRIM | ID: wpr-888559

ABSTRACT

OBJECTIVE@#To study the serological characteristics and molecular biological basis of 8 individuals with Para-Bombay phenotypes in Guangxi area.@*METHODS@#Serological tests were used to identify the blood groups of red cells. Molecular biological methods, including PCR-SSP for ABO genotyping and DNA sequencing for FUT1, were used to detect the genotypes of ABO and FUT1 which determined the expression of H antigen.@*RESULTS@#Eight individuals in the study were all the Para-Bombay phenotypes, including 4 cases of B@*CONCLUSION@#There are varieties of molecular genetic mechanisms for Para-Bombay phenotypes. In this study, the FUT1 mutations that cause Para-Bombay phenotypes in Guangxi area are mainly h3, h


Subject(s)
ABO Blood-Group System/genetics , Alleles , China , Fucosyltransferases/genetics , Genotype , Humans , Mutation , Phenotype
4.
Article in Chinese | WPRIM | ID: wpr-888064

ABSTRACT

Trigonella foenum-graecum is an annual plant of the genus Trigonella in the Leguminosae family. It is widely distributed in China and has a long history of application. According to phytochemistry research, the seeds, stem, and leaves of this herb contain not only a variety of bioactive ingredients, including alkaloids, saponins, polysaccharides, flavonoids, and phenols, but also abundant nutrients such as unsaturated fatty acids and amino acids and various trace elements. Pharmacological studies have shown that both the extract of T. foenum-graecum and its chemical constituents exhibit hypoglycemic, hypolipidemic, antitumor, antioxidative, antimicro-bial, and hepatoprotective activities. This paper reviews the research progress on the chemical constituents and pharmacological effects of T. foenum-graecum, which may contribute to further development, application, and clinical research of this herb.


Subject(s)
Antioxidants/pharmacology , Hypoglycemic Agents , Plant Extracts/pharmacology , Seeds , Trigonella
5.
Article in Chinese | WPRIM | ID: wpr-880108

ABSTRACT

OBJECTIVE@#To analyze the influence of serum homocysteine (Hcy) levels to the prognosis of newly diagnosed multiple myeloma (MM) patients, and to explore related factors affecting the prognosis of the patients.@*METHODS@#The clinical pathological data of 180 newly diagnosed MM patients treated in our hospital from March 2013 to February 2015 were collected, and the patients were divided into high and low Hcy groups based on the median Hcy. The survival curves of the patients in the two groups were drawn to compare the differences of the survival; univariate and multivariate survival analysis was used to observe the influence of serum cysteine to the prognosis of newly diagnosed MM patients; the clinicopathological data of the patients with high and low Hcy in the two groups was compared, Pearson test was used to further analyzes the relationship between Hcy and different factors, and explores the related factors of Hcy affecting the prognosis of the patients.@*RESULTS@#The median survival times of patients in the high and low Hcy groups were 32 (5-59) and 41 (7-71) months, respectively. The 3-year survival rate of the patients in high Hcy group was significantly lower than those in low Hcy group, and the difference shows statistically significant (P<0.05). The results of univariate survival analysis showed that the OS of newly diagnosed MM patients whom with advanced age, high bone disease grade, high-level bone marrow plasma cell count, LDH, C-reactive protein, Cr, β@*CONCLUSION@#Serum Hcy level has a correlation trend with the survival of newly diagnosed MM, which is affected by factors such as Hb.


Subject(s)
Bone Marrow Cells , Homocysteine , Humans , Multiple Myeloma , Prognosis , Risk Factors
6.
Article in Chinese | WPRIM | ID: wpr-873071

ABSTRACT

Gardenia has been widely used in clinical practice and with a long history. In order to make better use of gardenia resources,this study summarized historical literatures and modern researches,made textual research on the name,variety,origin,processing and efficacy of Gardenia jasminoides,and found that the name of G. jasminoides mainly reflects the characteristics of G. jasminoides based on form and color to a certain extent. In some cases, the name "Huangzhi" was mixed with water G. jasminoides. The earliest origin of G.jasminoides was Nanyang as recorded in ancient literatures, but with a low yield. Due to the influence of population migration and productivity development,the main origin of G. jasminoides moved to the south,and southern producers were preferred. G. jasminoides was recorded as G. jasminoides, and Gardeniae Fructus could not be used as medicine in literatures of Chinese materia medica,but there were records of "Fat G.jasminoides" and "Great G. jasminoides" as medicines in ancient books of treatment for febrile diseases. The reason for the difference with the literature records of this herb lay in different eras of "Leigong Bombardment Theory" and "Treatise on Febrile Diseases". There were gaps in the yield and harvesting capacity of G.jasminoides,and the herbaceous book was highly standardized, the highest-quality herbs were selected to be used as medicine,and the formularies focused on practicality. According to modern experimental studies,chemical composition and content of Gardeniae Fructus and Gardeniae Fructus were similar with the same choleretic effect. In addition,the processing history and efficacy application of G. jasminoides were also summarized,in order to provide reference for the further study and utilization of G. resources.

7.
Article | WPRIM | ID: wpr-833758

ABSTRACT

Blastocystis, an enteric protist, has been reported to be an important cause of protozoal gastrointestinal manifestations in humans and animals worldwide. Animals harboring certain Blastocystis subtypes (STs) may serve as a potential source of human infection. However, information about the prevalence and genetic diversity of Blastocystis in alpacas is limited. In the present study, a total of 366 fecal samples from alpacas in Shanxi Province, northern China, were examined for Blastocystis by PCR amplification of the small subunit rRNA gene, followed by sequencing and phylogenetic analysis. The prevalence of Blastocystis in alpacas was 23.8%, and gender difference in the prevalence of Blastocystiswas observed. The most predominant Blastocystis ST was ST10, followed by ST14 and ST5. The detection of ST5, a potentially zoonotic genotype, indicates that alpacas harboring ST5 could be a potential source of human infection with Blastocystis. These data provide new insight into the prevalence and genetic diversity of Blastocystis in alpacas.

8.
Article in English | WPRIM | ID: wpr-878305

ABSTRACT

Objective@#Several COVID-19 patients have overlapping comorbidities. The independent role of each component contributing to the risk of COVID-19 is unknown, and how some non-cardiometabolic comorbidities affect the risk of COVID-19 remains unclear.@*Methods@#A retrospective follow-up design was adopted. A total of 1,160 laboratory-confirmed patients were enrolled from nine provinces in China. Data on comorbidities were obtained from the patients' medical records. Multivariable logistic regression models were used to estimate the odds ratio ( @*Results@#Overall, 158 (13.6%) patients were diagnosed with severe illness and 32 (2.7%) had unfavorable outcomes. Hypertension (2.87, 1.30-6.32), type 2 diabetes (T2DM) (3.57, 2.32-5.49), cardiovascular disease (CVD) (3.78, 1.81-7.89), fatty liver disease (7.53, 1.96-28.96), hyperlipidemia (2.15, 1.26-3.67), other lung diseases (6.00, 3.01-11.96), and electrolyte imbalance (10.40, 3.00-26.10) were independently linked to increased odds of being severely ill. T2DM (6.07, 2.89-12.75), CVD (8.47, 6.03-11.89), and electrolyte imbalance (19.44, 11.47-32.96) were also strong predictors of unfavorable outcomes. Women with comorbidities were more likely to have severe disease on admission (5.46, 3.25-9.19), while men with comorbidities were more likely to have unfavorable treatment outcomes (6.58, 1.46-29.64) within two weeks.@*Conclusion@#Besides hypertension, diabetes, and CVD, fatty liver disease, hyperlipidemia, other lung diseases, and electrolyte imbalance were independent risk factors for COVID-19 severity and poor treatment outcome. Women with comorbidities were more likely to have severe disease, while men with comorbidities were more likely to have unfavorable treatment outcomes.


Subject(s)
Adult , Aged , COVID-19/virology , China/epidemiology , Comorbidity , Female , Humans , Male , Middle Aged , Retrospective Studies , Severity of Illness Index , Treatment Outcome
9.
Article in Chinese | WPRIM | ID: wpr-772658

ABSTRACT

OBJECTIVE@#To observe the clinical outcomes of a combined unilateral intraoral and extraoral reduction approach in the treatment of anterior temporomandibular joint (TMJ) dislocation.@*METHODS@#Postural muscular chains were utilized in the biomechanical analysis of stomatognathic systems for improving TMJ repositioning approaches. A total of 87 patients with anterior TMJ dislocation were included in the present study. A combined unilateral intraoral and extraoral reduction approach was applied, and the clinical effects were evaluated.@*RESULTS@#Biomechanical analysis reveal that reflexive contrac-tion of the maxillary muscle group was blocked sufficiently during the combined unilateral intraoral and extraoral reduction process. All dislocated TMJs were set successfully and efficiently with few complications.@*CONCLUSIONS@#Combined unilateral intraoral and extraoral reduction approach is an effective, convenient, and minimally invasive way to treat anterior TMJ dislo-cations.


Subject(s)
Humans , Joint Dislocations , Reconstructive Surgical Procedures , Temporomandibular Joint , Temporomandibular Joint Disorders , General Surgery
10.
Article in Chinese | WPRIM | ID: wpr-776540

ABSTRACT

OBJECTIVE@#To investigate the effects of Notch signal on hypoxic induction factor (HIF-1α) and autophagy-associated genes Beclin1, LC3I, LC3II in oxygen-glucose deprivation (OGD) induced myocardial cell injury.@*METHODS@#The OGD model was established using hypoxic culture box and hypoglycemic DMEM medium. The cells were divided into normal control group, OGD group, OGD + NC siRNA group, OGD + Notch1 siRNA group and OGD + HIF-1α siRNA group. Western blot was used to detect the interference effects of HIF-1α siRNA and Notch1 siRNA. The effects of Notch1 siRNA and HIF-1α siRNA on the activity of myocardial cells in OGD model were detected by the CCK-8 assay. The effects of Notch1 siRNA and HIF-1α siRNA on autophage-associated genes Beclin1, LC3I and LC3II expression were detected by Western blot.@*RESULTS@#The results of Western blot showed that HIF-1α siRNA could effectively knock down the expression of HIF-1α in myocardial cells in OGD model, and Notch1 siRNA could effectively knock down the expression of Notch1 and HIF-1α in myocardial cells in OGD model. The result of CCK-8 assay showed that Notch1 siRNA and HIF-1α siRNA reduced the activity of myocardial cells in OGD model, and there was no statistical difference between the two groups. Western blot results showed that Notch1 siRNA and HIF-1α siRNA could reduce the expressions of the autophagy-associated genes Beclin1, LC3I and LC3II, and reduce the ratio of LC3II to LC3I at mRNA level.@*CONCLUSION@#Notch1 plays a role in myocardial protection by regulating the expression of HIF-1α to regulate the autophagy in OGD model cells.


Subject(s)
Autophagy , Beclin-1 , Metabolism , Cell Hypoxia , Cells, Cultured , Glucose , Humans , Hypoxia-Inducible Factor 1, alpha Subunit , Metabolism , Microtubule-Associated Proteins , Metabolism , Myocytes, Cardiac , Cell Biology , Pathology , Oxygen , Receptors, Notch , Metabolism , Signal Transduction
11.
Journal of Chinese Physician ; (12): 85-88, 2019.
Article in Chinese | WPRIM | ID: wpr-734074

ABSTRACT

Objective To explore the clinical efficacy of biofeedback electrical stimulation plus pelvic floor muscle training (PFMT) in the treatment of stress urinary incontinence (SUI) after total hysterectomy.Methods The research objects who underwent total hysterectomy and complicated SUI were selected from January 2014 to December 2017 in our hospital.They were randomly divided into two groups:the study group (n =55) received biofeedback electrical stimulation plus PFMT treatment,while the control group (n=55) received PFMT treatment only.To record and compare the efficacy related indicators of the two groups.Results The indexes and scores of 3/6 months in the two groups were significantly improvedafter treatment.After 3 months the treatment,the study group had less leakage of urine [(4.3 ± 1.2)g vs (5.7 ± 1.9) g],better recovery of muscle strength of Ⅰ/Ⅱ muscle fibers [(3.5 ± 0.9) grade vs (2.8 ± 1.1) grade &(3.7 ± 0.6) grade vs (3.2 ± 1.2) grade],and better score of international consultation on incontinence questionnaire-short form (ICI-Q-SF) questionnaire [(2.7 ± 1.4) vs (4.5 ± 2.2)] compared with the control group,with statistically significant difference (P ≤ 0.05).After 6 months treatment,compared with the contiol group,the study group had less leakage of urine pad test [(1.4 ± 0.7) g vs (2.6 ± 1.1) g],better recovery of muscle strength of type Ⅰ/Ⅱ muscle fiber [(4.2 ±0.8) grade vs (3.5 ± 1.1)grade & (4.7 ±0.5) grade vs (4.0 ±0.9)grade],and better ICI-Q-SF score [(2.7 ± 1.4) vs (4.5 ±2.2)] (P ≤0.05).Conclusions Compared with using the PFMT alone,biofeedback electrical stimulation plus PFMT treatment had less leakage,better pelvic floor muscle strength and better subjective symptoms.

12.
Article in Chinese | WPRIM | ID: wpr-905732

ABSTRACT

This study described the significance of professional education in physical therapy in Capital Medical University School of Rehabilitation Medicine under the background of the World Confederation for Physical Therapy (WCPT) International Course Accreditation, analyzed the present problems of core curriculum system as well as the inevitable trends of carrying on the education reform of physical therapy, and the continuous reform program of core curriculum system in physical therapy based on the accreditation of the WCPT were suggested. The final purpose was to improve the quality of physical therapy talents substantially in order to achieve the standard of WCPT accreditation.

13.
Article in Chinese | WPRIM | ID: wpr-802523

ABSTRACT

Objective: To investigate the effective substance basis and possible mechanism of Huangliantang in treatment of gastritis. Method: Integrated pharmacology platform of traditional Chinese medicine was employed to predict the main active ingredients and functional targets of Huangliantang in treatment of gastritis, network of composition target-disease target of Huangliantang was constructed, key nodes were screened for enrichment analysis of pathways, and the possible mechanism of Huangliantang in treatment of gastritis with multiple ingredients-multiple targets-multiple pathways was explored. Result: A total of 175 predicted active ingredients of Huangliantang interacted with 538 key targets about gastritis, the regulation and treatment of gastritis during its different pathological stages, such as Helicobacter pylori infection, gastric mucosal damage and gastric mucosal atrophy, were involved through chemokine, T cell receptor, estrogen and other signaling pathways. Conclusion: This research may reveal the potential active ingredients of Huangliantang in treatment of gastritis and its possible mechanism, and it also provides a theoretical basis for further experimental research of pharmacodynamic substance basis and mechanism of action.

14.
Article in Chinese | WPRIM | ID: wpr-801928

ABSTRACT

Objective: Harmonization method is one of the eight unique methods of traditional Chinese medicine with important application value in clinic. Based on the effect of harmonization method in regulating cold and heat, the mechanism of Huangliantang in treating chronic non-atrophic gastritis(CNAG) on rats were studied. Method: Rats were divided into normal group (n=10) and CNAG model group (n=50). The model of CNAG rats was induced by chemical stimulation combined with hunger and satiety. The model group was randomly divided into 5 groups, namely the model group, the Jinghua Weikang pill treatment group, and the high, middle and low-dose Huangliantang groups, with 8 rats in each group. After the model was successfully established, the Jinghua Weikang pill treatment group (0.04 g·kg-1), the high, middle, low dose Huangliantang group (11.00,5.48,2.74 g·kg-1), the blank group and the model group were given the same dose of saline for 4 weeks, and then the samples were collected. The histological changes of gastric mucosa were observed by hematoxylin-eosin (HE) staining. The serum levels of interleukin-6 (IL-6), interleukin-1β (IL-1β) and interleukin-8 (IL-8) were measured by enzyme-linked immunosorbent assay (ELISA). immunohistochemistry(IHC) was used to detect nuclear factor-κB (NF-κB) and its inhibitory protein receptor (IκBα), protein expression. Real-time quantitative fluorescence polymerase chain reaction (Real-time PCR) was used to detect IκBα, NF-κB mRNA expressions. Result: In the model group, the gastric mucosa was damaged, a large number of inflammatory cells were infiltrated, the serum inflammatory factors increased significantly, mRNA and protein expressions of IκBα decreased, and mRNA and protein expressions of NF-κB increased in the gastric tissue (PκBα was up-regulated, IκBα protein was increased, while the expression of NF-κB mRNA was down-regulated, and NF-κB protein was decreased. The Jinghua Weikang pill treatment group and the high-dose Huangliantang group had the most obvious improvement (PPConclusion: Huangliantang for regulating cold and heat based on the harmonization method can effectively alleviate the degree of gastric mucosal injury, and reduce serum inflammatory factors in CNAG rats. The mechanism is related to the up-regulation of IκBα mRNA expression, and the down-regulation of NF-κB mRNA expression and NF-κB protein expression in gastric mucosa.

15.
Article in Chinese | WPRIM | ID: wpr-849927

ABSTRACT

Objective To analyze the serum polypeptide fingerprints of Enterococcus faecium (E. faecium) bloodstream infection in mice at different time points using matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-FOF MS), screen differential polypeptide peaks, and create a corresponding diagnostic model and look for new markers. Methods Ninety ICR mice were used to establish E. faecium bloodstream infection model (including 10 mice as normal control group with PBS injection, and 80 mice as the infection group with 1/2 LD50 E. faecium injection in a volume of 0.1 ml/10 g by the intravenous tail). Blood samples were collected at 8 time points (1, 3, 6, 12, 24, 48, 96 and 128 h), and tested for IL-1α, IL-1β and IL-6 detection. Serum samples were then purified with weak cation magnetic beads, the serum polypeptide profiling was recognized by MALDI-TOF MS and BioExplorer software between infection group and normal control group. Amino acid sequences of the candidate polypeptides were identified by nano-liquid chromatography electrospray ionization-tandem mass spectrometry (nano-LC/ESI–MS/MS). Results After infected with E. faecium, the mice showed abnormal behaviors compared with the normal control group, including listless and reduced activity; IL-1α was down-regulated, while IL-1β and IL-6 were up-regulated. A total of 102 polypeptide peaks were detected by fingerprints analysis. Screening on the basis of 5 times difference in content in both infection group and control group, 8 polypeptides were higher in infection group than those in control group (P<0.01), and 9 polypeptides were lower in infection group than those in control group (P<0.01). Combining five polypeptide peaks of m/z 1227.4, 1512.9, 4509.7, 5007.3 and 7816.7, the corresponding diagnostic model was established with accuracy rate of 80%, specificity of 76.6% and sensitivity of 83.3%. Identified by secondary mass spectrometry, polypeptide peaks m/z 1227.4 and 5007.3 were the fragment of β2microglobulin and complement C3, respectively. Conclusions MALDI-TOF MS combined with BioExplorer software, when used as a new method to study the E. faecium bloodstream infection, may find the differential polypeptides between infection group and control group, and can effectively establish a diagnostic model of bloodstream infection. β2-microglobulin and complement C3 are expected to become the new biomarkers for auxiliary diagnosis of bacterial bloodstream infection.

16.
China Pharmacy ; (12): 99-103, 2019.
Article in Chinese | WPRIM | ID: wpr-816758

ABSTRACT

OBJECTIVE: To provide a theoretical basis for the development of the following products of Coptis chinensis- Zingiber ojjicinale couplet medicine (“Coptis chinensis-Zingiber ojjicinale” for short) prescription. METHODS: The prescriptions containing Coptis chinensis-Zingiber ojjicinale were collected from the Dictionary of TCM Prescription and input into TCM inheritance support platform software (V2.5) to establish the database. The frequency of major diseases and compatibility medicinal materials were analyzed statistically. The core combination of medicinal materials in the prescriptions containing Coptis chinensis-Zingiber ojjicinale were analyzed statistically with association rule Apriori algorithm (support degrees were 15%, 20%, 25%, confidence was 0.90). Top 2 main diseases in the list of frequency, compatibility medicinal materials for dispelling internal cold and medicine for clearing heat with highest compatibility frequency were selected and analyzed in respect of prescription rules. RESULTS: A total of 492 prescriptions containing Coptis chinensis-Zingiber ojjicinale were screened, 9 kinds of major diseases (frequency≥15), such as dysentery, diarrhea, accumulation, fullness. There were 21 commonly used compatibility medicinal materials (frequency≥55), including Angelica sinensis-Panax ginseng, Magnolia officinalis, Aconitum carmichaelii, Scutellaria baicalensis, etc. There were 19 commonly used medicinal materials combinations, including Coptis chinensis-Zingiber ojjicinale-Panax ginseng, Coptis chinensis-Zingiber ojjicinale- Magnolia officinalis, Coptis chinensis-Zingiber ojjicinale-Angelica sinensis. There are 5 kinds of core medicinal materials commonly used in treating dysentery with Coptis chinensis-Zingiber ojjicinale,and 9 kinds of core medicinal materials for treating dysentery. There are 8 kinds of core medicinal materials in Coptis chinensis-Zingiber ojjicinale compatible with medicine for dispelling internal cold Aconitum carmichaelii prescription.and 7 core medicinal materials in compatible with medicine for clearing heat Scutellaria baicalensis prescription. CONCLUSIONS: The major diseases treated with prescriptions containing Coptis chinensis-Zingiber ojjicinale are mainly digestive tract diseases. It can treat different diseases being compatible with different medicinal materials, this study aslo can provide theoretical basis for the development of subsequent products.

17.
Article in Chinese | WPRIM | ID: wpr-694071

ABSTRACT

Objective To analyze the fingerprints of serum peptides in bloodstream infection induced by Candida albicans (C.albicans),with matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS),and establish the corresponding diagnostic model Methods To establish ICR mice model of C.albicans bloodstream infection,and collect the serum samples which were purified by weak cation exchange beads.The serum peptide finger printing was recognized by MALDITOF MS,and BioExplorer software was used for analysis between infection group and normal control group.Furthermore,the peptide fingerprints were analyzed between C.albicans infection group,Escherichia coli (E.coli) infection group and Staphylococcus aureus (S.aureus) infection group.Results Comparison between C.albicans infection group and normal control group,there were 135 polypeptide peaks,of which 5 polypeptides up-regulated,6 down-regulated and 7 up-regulated first and down-regulated afterwards.The diagnostic model was established by combination of 6 peaks (i.e.m/z 1610.9,1742.3,2666.4,2778.0,3345.1 and 4528.8),which possessed an accurate rate of 80.0% in diagnosis of C.albicans infection.It was found by comprehensive comparison between C.albicans,E.coli and S.aureus infection groups that there were 5 polypeptides expressed collectively,i.e.m/z1513.8,2910.8,3538.1,3884.9 and 5007.3.Conclusions MALDI-TOF MS can be used to distinguish the C.albicans infection and normal polypeptide peaks.The collective polypeptides expressed in the infection groups can be further used to diagnose the infection.Establishment of corresponding diagnostic models may be helpful for early diagnosis of fungal bloodstream infection and provide a basis for reasonable clinical medication.

18.
Article in Chinese | WPRIM | ID: wpr-701631

ABSTRACT

Objective To investigate the changes in CD8+T lymphocyte count and effect on immune function in patients with sepsis after continuous renal replacement therapy (CRRT).Methods General data of septic patients who admitted to the emergency department of a hospital between October 2015 and August 2016 were collected,pe-ripheral blood was taken from patients before and after single CRRT,the total CD8+T cell count,interferon-γ(IFN-γ)-secreting CD8+T cell count,the levels of inhibitory molecules and costimulatory molecules as well as IFN-γand tumor necrosis factor-α(TNF-α)produced by CD8+T cells were detected.Results A total of 37 hospitalized septic patients were infected with gram-negative bacteria, pathogens causing infection were Klebsiella pneumoniae (22 strains),Acinetobacter baumannii (1 1 strains),and Enterobacter cloacae (3 strains).After CRRT,the body temperature,heart rate,white blood cell count,urea nitrogen,and serum creatinine levels in septic patients were all lower than those before CRRT (all P<0.05).After CRRT,the total CD8+T cell count in septic patients didn’t change significantly (P>0.05),but IFN-γ-secreting CD8+T cell count increased (P<0.05).Levels of cytotoxic T lymphocyte-associated antigen 4(CTLA-4),programmed death-1 (PD-1),T-cell immunoglobulin and mucin-do-main-containing molecule 3 (TIM-3)after CRRT were all lower than those before CRRT (all P<0.05 ),while levels of costimulatory molecules CD28 and secreting IFN-γ elevated after CRRT(all P<0.05).Conclusion CRRT can not only improve the vital signs and renal function of patients with sepsis,but also enhance the immune function of CD8+T cells.

19.
Article in Chinese | WPRIM | ID: wpr-698794

ABSTRACT

BACKGROUND: Oxidative stress is one of the important mechanisms of postoperative abdominal adhesion. The nuclear factor-E2-related factor 2 (Nrf2)-antioxidant response element (ARE) signal pathway is an important endogenous anti-oxidation stress pathway. Our previous study found that ligustrazine nano-spray can inhibit the formation of postoperative abdominal adhesion in rats, and moreover, ligustrazine has an anti-oxidation effect. OBJECTIVE: To observe the effect of ligustrazine nano-spray on the expression of mRNAs and proteins related to the Nrf2-ARE signal pathway in rats with abdominal adhesion, and to investigate the mechanism by which ligustrazine nano-spray inhibits abdominal adhesion via regulating the Nrf2-ARE signal pathway. METHODS: Sprague-Dawley rats were randomly divided into sham group, model group, ligustrazine group and sodium hyaluronate group. In the sham group, only laparotomy was performed without modeling. In the model group, an abdominal adhesion model was created but no drug was used. In the ligustrazine group, ligustrazine nano-spray was used on the wound before incision suturing. In the sodium hyaluronate group, sodium hyaluronate was applied on the wound before incision suturing. RESULTS AND CONCLUSION: Compared with the model group, ligustrazine nano-spray reduced the levels of reactive oxygen species, tissue inhibitor of matrix metalloproteinase 1, but increased the level of matrix metalloproteinase 9 in the rat serum. The expression of Nrf2 and HO-1 mRNA and proteins was also up-regulated in the ligustrazine group relative to the model group. Therefore, ligustrazine nano-sprays can inhibit abdominal adhesions in rats, and its mechanism may be related to the regulation of Nrf2 and heme oxygenase 1 mRNA and proteins expression and the activation of Nrf2-ARE signaling pathway.

20.
Article in Chinese | WPRIM | ID: wpr-696227

ABSTRACT

Objective To establish of a close loop blood information system forreal time monitoring in the entire process of clinical blood transfusion.Methods In accordance with files and laws in clinical blood transfusion and combining the work in blood transfusion department with the actual needs of clinical blood transfusion,the blood transfusion department in Shaanxi Provinical People's Hospital developed the close-loop blood information system with Skynet Software Co.Ltd.Results The close-loop blood information system was achieved informational management patternthat was detailed information from blood products arrival to departure and whole process of evaluation transfusion effect.The link of Hospital Information Manage System (HIS),Laboratory Information System (LIS) and blood information system was completed with the system.The system realized real-time and accurate data collection and monitoring.The system achieved a closed-loop management of the entire process of clinical blood transfusion.Conclusion By using theclose loop blood information system,the quality of medical services can be improved,the work productivity can be increased,and the clinical blood transfusion can be rationalized.

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