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1.
Acta cir. bras ; 35(12): e351204, 2020. tab, graf
Article in English | LILACS | ID: biblio-1152679

ABSTRACT

Abstract Purpose: To evaluate the effect of diode laser use on experimental orthodontic tooth movements. Methods: Thirty Rattus norvegicus albinus Wistar were divided into three equal groups (n = 10), two experimentals and one control. Applying 20 g orthodontic force were attached to the maxillary incisors of the rats in all groups. Low dose laser was applied to the surrounding tissues of the maxillary incisors of the rats in the experimental groups. Two exposure times for laser irradiation were used for seven days: t = 12 min (energy dose = 72 J) and t = 9 min (energy dose = 54 J) by a 0.1 W DEKA brand diode laser with wavelength of 980 nm. Results: Osteoclastic activation increased in the 72 J group when compared to control group and decreased in comparison to the 54 J group. Osteoblastic activation was decreased in the 72 J group when compared to the control group and increased in comparison to the 54 J group. Conclusions: Applying 54 J laser energy has been found effective to accelerate the orthodontic tooth movement.


Subject(s)
Tooth Movement Techniques , Low-Level Light Therapy , Osteoclasts , Rats, Wistar , Lasers, Semiconductor
2.
Acta cir. bras ; 35(4): e202000406, 2020. tab, graf
Article in English | LILACS | ID: biblio-1130636

ABSTRACT

Abstract Purpose To investigate the role of Rosmarinic acid (RA) in the prevention of traumatic brain injury and the immunohistochemical analysis of IBA-1 and GFAP expressions. Methods Healthy male rats were randomly divided into 3 groups consisting of 10 rats. Groups were as follows; control group, traumatic brain injury (TBI) group, and TBI+RA group. After traumatic brain injury, blood samples were taken from the animals and analyzed with various biochemical markers. And then IBA-1 and GFAP expressions were evaluated immunohistochemically. Results Significant results were obtained in all biochemical parameters between groups. Immunohistochemical sections showed IBA-1 not only in microglia and macrophage activity but also in degenerative neurons in blood vessel endothelial cells. However, GFAP reaction and post-traumatic rosmarinic acid administration showed positive expression in astrocytes with regular structure around the blood vessel. Conclusion Rosmarinic acid in blood vessel endothelial cells showed that preserving the integrity of astrocytic structure in the blood brain barrier may be an important antioxidant.


Subject(s)
Animals , Male , Calcium-Binding Proteins/analysis , Cinnamates/pharmacology , Craniotomy/methods , Depsides/pharmacology , Brain Injuries, Traumatic/prevention & control , Glial Fibrillary Acidic Protein/analysis , Microfilament Proteins/analysis , Reference Values , Immunohistochemistry , Random Allocation , Astrocytes/drug effects , Reproducibility of Results , Rats, Sprague-Dawley , Neuroprotective Agents/pharmacology , Brain Injuries, Traumatic/surgery , Brain Injuries, Traumatic/pathology , Glutathione Peroxidase/analysis , Malondialdehyde/analysis
3.
Braz. oral res. (Online) ; 34: e035, 2020. tab, graf
Article in English | LILACS, BBO | ID: biblio-1100934

ABSTRACT

Abstract Although fiber-reinforced composites are commonly used in dental practice, whether fiber-reinforced crowns and fixed partial dentures can be used as definitive prostheses remains to be determined. This study used scanning electron microscopy to evaluate the load-bearing capacity of non-reinforced and fiber-reinforced composite (FRC) molar crowns prepared by computer-aided design/computer-aided manufacturing (CAD/CAM). The crowns were fabricated from three empirical FRC blocks, one empirical composite block, and one commercial ceramic block. The FRC resin was prepared by mixing BaO silicate particles, E-glass fiber, and dimethacrylate resin. Specimens were divided into five groups (n = 10), differing in the amounts of filler, resin, and fiber. Crowns were statically loaded until fracture. One-way analysis of variance and Tukey's post hoc multiple comparison tests were used for statistical analyses. The groups showed significant differences in load-bearing capacity; empirical bidirectional FRC resin blocks had the highest capacity, while commercial ceramic blocks had the lowest capacity. Molar crowns formed from FRC resin blocks had higher load-bearing capacity compared to non-reinforced composite resin and ceramic blocks. These results show that fiber reinforcement increased the load-bearing capacity of molar crowns.


Subject(s)
Humans , Weight-Bearing , Computer-Aided Design , Composite Resins/chemistry , Crowns , Reference Values , Surface Properties , Materials Testing , Microscopy, Electron, Scanning , Ceramics/chemistry , Reproducibility of Results , Dental Prosthesis Design , Evaluation Study , Molar
4.
Acta cir. bras ; 34(9): e201900904, 2019. tab, graf
Article in English | LILACS | ID: biblio-1054694

ABSTRACT

Abstract Purpose: Ganoderma lucidum, a kind of mushroom used for its antioxidant, anti-inflammatory, and immunomodulatory activities, was investigated in the present study for its possible healing effect on calvarial defects with bone grafts. Methods: Wistar male rats (n = 30) were divided into 3 groups: 1) the control (defect) group (n = 10), 2) defect and graft group (n = 10), and 3) defect, graft, and G. lucidum treated group (n = 10). The G. lucidum was administered to the rats at 20 mL/kg per day via gastric lavage. Results: In the defect and graft group, osteonectin positive expression was observed in osteoblast and osteocyte cells at the periphery of the small bone trabeculae within the graft area. In the defect, graft, and G. lucidum treated group, osteonectin expression was positive in the osteoblast and osteocyte cells and positive osteonectin expression in new bone trabeculae. The expression of matrix metalloproteinase-9 (MMP-9) was positive in the inflammatory cells, fibroblast cells, and degenerated collagen fibril areas within the defect area. Conclusion: This study shows that, with its antioxidant and anti-inflammatory properties, G. Lucidum is an important factor in the treatment of calvarial bone defects.


Subject(s)
Animals , Male , Rats , Skull/surgery , Bone Regeneration/drug effects , Bone Transplantation , Reishi/chemistry , Anti-Inflammatory Agents/therapeutic use , Antioxidants/therapeutic use , Skull/drug effects , Immunohistochemistry , Osseointegration/drug effects , Rats, Wistar , Disease Models, Animal
5.
Acta cir. bras ; 34(4): e201900408, 2019. tab, graf
Article in English | LILACS | ID: biblio-1001086

ABSTRACT

Abstract Purpose: To evaluate histologically and immunohistochemically the bone regeneration after application of simvastatin on tibial bone defects in rats. Methods: Sixty Wistar albino rats were divided into 3 groups as control (6 mm tibial bone defect), defect + graft (allograft treatment), and defect + graft + simvastatin (10 mg/kg/day) for 28 days. Results: Histopathological examination revealed inflammation in control group (defect group), congestion in blood vessels, and an increase in osteoclast cells. In defect + graft group, osteoclastic activity was observed and osteocyte cells were continued to develop. In defect + graft + simvastatin group, osteocytes and matrix formation were increased in the new bone trabeculae. Osteopontin and osteonectin expression were positive in the osteclast cells in the control group. Osteoblasts and some osteocytes showed a positive reaction of osteopontin and osteopontin. In defect + graft + simvastatin group, osteonectin and osteopontin expression were positive in osteoblast and osteocyte cells, and a positive expression in osteon formation was also seen in new bone trabeculae. Conclusion: The simvastatin application was thought to increase bone turnover by increasing the osteoinductive effect with graft and significantly affect the formation of new bone.


Subject(s)
Animals , Male , Rats , Tibia/drug effects , Bone Regeneration/drug effects , Simvastatin/pharmacology , Osteoblasts , Osteoclasts , Tibia/surgery , Tibia/pathology , Bone Remodeling/drug effects , Rats, Wistar , Disease Models, Animal , Autografts
6.
Acta cir. bras ; 34(3): e201900306, 2019. tab, graf
Article in English | LILACS | ID: biblio-989063

ABSTRACT

Abstract Purpose: To investigate the effects of allopurinol administration on osteoinductive reaction and bone development with graft material. Methods: Thirty-six Wistar albino rats were divided into 3 groups. In the control group, calvarial bone defect was only created without any treatment. In the Defect + Graft group, allograft treatment was performed by forming 8 mm calvarial bone defect. In the Defect + Graft + Allopurinol group, alloplastic bone graft was placed in the calvarial bone defect and then, allopurinol (50 mg/kg/day) treatment was intraperitoneally applied for 28 days. Results: Histopathological examination revealed inflammation, congestion in the vessels, and an increase in osteoclast cells in the defect area. We also observed that new osteocyte cells, increase in connective tissue fibers, and new bone trabeculae. Osteopontin expression was positive in osteoblast cells and lacunated osteocyte cells were located in the periphery of the new bone trabeculae. Osteopontin expression was also positive in osteoblasts and osteocytes cells of new bone trabeculae in the graft site. Conclusion: It has been shown that allopurinol treatment in rat calvaria defects may induce osteoblastic activity, matrix development, mature bone cell formation and new bone formation when used with autogenous grafts.


Subject(s)
Animals , Rats , Osteogenesis/drug effects , Skull/drug effects , Wound Healing/drug effects , Bone Regeneration/drug effects , Allopurinol/pharmacology , Skull/injuries , Rats, Wistar , Disease Models, Animal , Autografts
7.
Int. j. morphol ; 36(4): 1453-1462, Dec. 2018. tab, graf
Article in English | LILACS | ID: biblio-975722

ABSTRACT

Traumatic brain injury (TBI) can potentially lead to hemorrhages in all areas of the skull, which can damage cells and nerve connections. This study aims to investigate the protective effects of Ganoderma lucidum polysaccharides (GLPS) as a antioxidant on cerebellar cell tissues after traumatic brain injury in rats. Sprague Dawley rats were subjected to TBI with a weight-drop device using 300 g1m weight-height impact. The groups are consisted of control, trauma, and trauma+Ganoderma lucidum groups. At seven days post-brain injury, experimental rats were decapitated after intraperitoneal administration of ketamine HCL (0.15 ml/100 g body weight). Cereballar samples were taken for histological examination or determination of malondialdehyde (MDA) and glutathione (GSH) levels and myeloperoxidase (MPO) activity. Significant improvement was observed in cells and vascular structures of Ganoderma lucidum treated groups when compared to untreated groups. It is believed that Ganoderma lucidum may have an effect on the progression of traumatic brain injury. Ganoderma lucidum application may affect angiogenetic development in blood vessel endothelial cells, decrease inflammatory cell accumulation by affecting cytokine mechanism and may create apoptotic nerve cells and neuroprotective mechanism in glial cells.


La lesión cerebral traumática (LCT) puede provocar hemorragias en todas las áreas del cráneo, lo que puede dañar las células y las conexiones nerviosas. Este estudio tuvo como objetivo investigar los efectos protectores de los polisacáridos de Ganoderma lucidum (GLPS) como antioxidante en los tejidos de las células del cerebelo después de la lesión cerebral traumática en ratas. Ratas Sprague Dawley fueron sometidas a TBI con un dispositivo de caída de peso usando un impacto de peso de 300 g-1 m. Se formaron los siguientes grupos: control, trauma y trauma + Ganoderma lucidum. Siete días después de la lesión cerebral, las ratas experimentales fueron decapitadas después de la administración intraperitoneal de ketamina HCL (0,15 ml / 100 g de peso corporal). Se tomaron muestras cerebrales para el examen histológico y para la determinación de niveles de malondialdehído (MDA) y glutatión (GSH) y actividad de mieloperoxidasa (MPO). Se observó una mejora significativa en las células y las estructuras vasculares de los grupos tratados con Ganoderma lucidum en comparación con los grupos no tratados. Durante el estudio se observó que Ganoderma lucidum puede tener un efecto sobre la progresión de la lesión cerebral traumática. La aplicación de Ganoderma lucidum puede afectar el desarrollo angiogénico en las células endoteliales de los vasos sanguíneos, disminuir la acumulación de células inflamatorias al afectar el mecanismo de las citocinas y puede crear células nerviosas apoptóticas y un mecanismo neuroprotector en las células gliales.


Subject(s)
Animals , Male , Rats , Cerebellum/drug effects , Reishi/chemistry , Brain Injuries, Traumatic/pathology , Antioxidants/pharmacology , Polysaccharides/pharmacology , Immunohistochemistry , Antigens, Differentiation, Myelomonocytic , Antigens, CD , Cerebellum/metabolism , Cerebellum/pathology , Blotting, Western , Rats, Sprague-Dawley , Peroxidase/metabolism , Neuroprotective Agents , Proto-Oncogene Proteins c-bcl-2 , Vascular Endothelial Growth Factor A/metabolism , Glutathione/analysis , Malondialdehyde/analysis
8.
Int. j. morphol ; 36(2): 488-492, jun. 2018. tab, graf
Article in English | LILACS | ID: biblio-954142

ABSTRACT

Spinal cord injury causes neuron nerve fiber loss. The aim of this study was to investigate the neuroprotective, inflammatory and angiogenetic effects of melatonin on rat spinal cord injury (SCI). For spinal cord injury, a standard weight reduction method was used that caused moderate severity of injury (100 g / cm force) at T10 Melatonin (10 mg/kg intraperitoneally ) was administered for 10 days after trauma. Each group consisted of 10 animals. of these, six were used for biochemical and four were used for the evaluation of histological analysis. Spinal cord samples were taken for histological examination or determination of malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity. Spinal cord injury and melatonin treated group were compared. Melatonin administration in spinal cord injury increased the activity of glial cells in the radial and funicular cells and ependymal cells and increased the activity of glial cells and also showed a positive effect on inflammation and vascular endothelial cells in synaptic connections in the nerve fibers undergoing spinal injury endothelial degeneration It is thought that it can regulate the degenerative effect which is caused by both the inflammatory effect and the angiogenic effect which will have a positive effect on the neural connection.


La lesión de la médula espinal (SCI) provoca daño en la fibra nerviosa, que puede conducir a alteraciones motoras y sensitivas, incluso la muerte. El objetivo de este estudio fue investigar los efectos neuroprotectores, proinflamatorios y proangiogénicos de la melatonina en un modelo de SCI inducida en rata. Para tal efecto se utilizaron dos grupos: Grupo 1 (n:10) se le indujo una SCI, mediante el método de reducción de peso estándar (100 g/cm fuerza), provocando una lesión de severidad moderada. Grupo 2 (n:10) inducción SCI más aplicación de T10 Melatonina (10 mg / kg v.i.) durante 10 días después del trauma. Muestras de seis animales de cada grupo fueron usados para análisis bioquímicos y los otros cuatro para la evaluación histológica. Se tomaron muestras de médula espinal para el examen histológico y para la determinación de niveles de malondialdehído (MDA) y glutatión (GSH), actividad mieloperoxidasa (MPO) y se comparó la lesión de la médula espinal y el grupo tratado con melatonina. La administración de melatonina en la lesión de la médula espinal aumentó la actividad de las células gliales en las células radiales, funiculares y ependimocitos. Ademas mostró un efecto positivo sobre la inflamación y angiogénesis en las conexiones sinápticas en las fibras nerviosas sometidas a lesión espinal. Pudiendo este participar en la regulación del efecto degenerativo causado, principalmente, por acción de angiogénesis e inflamación local.


Subject(s)
Spinal Cord Injuries/metabolism , Spinal Cord Injuries/drug therapy , Melatonin/metabolism , Melatonin/therapeutic use , Immunohistochemistry , Tumor Necrosis Factor-alpha/metabolism , Endothelin-1/metabolism
9.
Int. j. morphol ; 28(2): 357-363, June 2010. ilus
Article in English | LILACS | ID: lil-577120

ABSTRACT

Absence of the vertebral artery is rare, and incidentally encountered in radiological imaging technics. We reported a 74 years old man suffering from pulsatile tinnitus with absence of the left vertebral artery. The purpose of the case report is the description absence of the vertebral artery causing of pulsatile tinnitus, in order to offer useful data to anatomists, otorhinolaryngologist, radiologists, vascular, head and neck surgeons.


La ausencia de la arteria vertebral es rara, y accidentalmente encuentrada en técnicas de imagen radiológica. Reportamos un hombre de 74 años que sufre de tinnitus pulsátil con la ausencia de la arteria vertebral izquierda. El propósito del reporte del caso es la descripción de la ausencia de arteria vertebral que causante del tinitus pulsátil, con el fin de ofrecer datos útiles para anatomistas, otorrinolaringólogos, radiólogos, cirujanos vasculares y de cabeza y cuello.


Subject(s)
Humans , Male , Aged , Tinnitus/etiology , Vertebral Artery/abnormalities , Magnetic Resonance Angiography
10.
Int. j. morphol ; 27(3): 791-800, sept. 2009. ilus
Article in English | LILACS | ID: lil-598938

ABSTRACT

The objective of this study was to evaluate morphological changes of the median nerve in patients with carpal tunnel syndrome (CTS) and healthy controls, to correlate the MRI findings of wrists. This study compared not only morphological changes of the median nerve and also displayed descriptively structures in carpal tunnel between patients diagnosed with idopathic CTS and healthy controls. Our study involved 60 hand, 30 of hand were evaluated diagnosed with idiopathic CTS and 30 hand as healthy controls bilaterally. Two provocative tests (Phalen's and Tinel's test) were performed on each hand for both the patient group (60 wrist) and the control group (60 wrist). With regard to Phalen and Tinel's test results, 24 and 26 wrists were excluded from patient and control groups respectively. Totally 70 wrists were evaluated, and in terms of cross-sectional area of median nerve at the level of distal radio-ulnar joint, pisiform bone and the hook of hamate bone by MRI in the patient and control groups. In addition to evaluation of cross-sectional area of median nerve, we determined signal intensity of wrists and different localization of the median nerve in the carpal tunnel. Cross-sectional area of the median nerve measured by wrist magnetic resonance at the level of metacarpal bones and signal intensity of wrists may be considered as a valuable indicator to determine patients referred with idiopathic CTS.


El objetivo de este estudio fue evaluar los cambios morfológicos del nervio mediano en pacientes con síndrome del túnel carpiano (STC) y controles sanos, para correlacionar los hallazgos de las RM de muñeca. Este estudio comparó no sólo los cambios morfológicos del nervio mediano, también se muestran en forma descriptiva estructuras del túnel carpiano entre los pacientes diagnosticados con STC idiopatico y controles sanos. Nuestro estudio incluyó 60 manos, 30 manos fueron evaluados con diagnóstico de STC idiopático y 30 manos como controles sanos, bilateralmente. Dos pruebas de provocación (prueba de Phalen y prueba de Tinel) se realizaron en cada mano para el grupo de pacientes (60 muñecas) y el grupo control (60 muñecas). En lo que respecta a los resultados de prueba de Phalen y prueba de Tinel, 24 y 26 muñecas fueron excluidas del grupo paciente y grupo control respectivamente. En total 70 muñecas fueron evaluadas, en términos de la sección transversal del área del nervio mediano a nivel de la articulación radio-ulnar distal, el hueso pisiforme y el hamulus del hueso hamate por medio de resonancia magnética en gruposs paciente y control. Además de la evaluación de la sección del área del nervio mediano, se determinó la intensidad de la señal de la muñeca y las diferentes localizaciones del nervio mediano en el túnel carpiano. La sección transversal del área del nervio mediano medida por medio de resonancia magnética de muñeca a nivel de los huesos metacarpianos y la intensidad de la señal de las muñecas pueden ser considerados como un valioso indicador al evaluar pacientes referidos con STC idiopático.


Subject(s)
Humans , Female , Wrist/anatomy & histology , Wrist/innervation , Wrist , Carpal Tunnel Syndrome/diagnosis , Carpal Tunnel Syndrome/etiology , Carpal Tunnel Syndrome , Outcome Assessment, Health Care/methods , Magnetic Resonance Imaging/methods , Ulnar Nerve/anatomy & histology , Ulnar Nerve , Median Nerve/anatomy & histology , Median Nerve/injuries , Median Nerve , Radial Nerve/anatomy & histology , Radial Nerve , Paresthesia/diagnosis
11.
Int. j. morphol ; 27(2): 285-294, June 2009. ilus
Article in English | LILACS | ID: lil-563071

ABSTRACT

We aimed to investigate the potential harmful effects of maternal valproic acid (VPA) on fetal sciatic nerve, and the protective effects of vitamin E (Vit E) and folic acid (FA) on fetal rats. Valproic acid (400mg/kg), folic acid (400mg/kg) and vitamin E (250 mg/kg) were administered to rats on each of gestation days 8-10. All fetuses were collected on gestation day 20. With thin sections of biopsies, sciatic nerve of fetuses were stained with uranyl acetat and were examined under transmission electron microscope. The fetuses (n:36) were divided into five groups: control, vpa, vpa+fa, vpa+vit e and vpa+fa+vit e groups. In each group; drug procedure, surgical procedure and histological methods were performed. Later, weights and lengths of fetuses in each group were compared and analyzed by One-Way Anova test. Administration of single doses of valproic acid (400 mg/kg) resulted in weight and length loss between control and vpa group. However, length and weight differences between the other groups were not significant. The histopathological findings of control group was normal. In vpa group, it showed extensive degenerative changes especially in myelin coat. In addition, most prominent finding in this group was condensation of collagen fibers in extensively demyelinated samples, while moderately effected areas were relatively normal. Both vpa+fa and vpa+ vit e groups exhibited similar ultrastructural changes, reflecting minimal to moderate degenerative changes. In vpa+fa+vit e group had almost the normal structure. Administration of single doses of valproic acid (400 mg/kg) resulted in a deteriorative effect on sciatic nerve at ultrastructural level. Administration of FA and Vit E had a protective effect to prevent the degenerative changes to a certain degree. Combination of FA and Vit E together following VPA administration had a more potent protective effect. The objective of the present study is to analyze histopathologic changes which ...


El objetivo fue investigar los posibles efectos perjudiciales del ácido valproico (AVP) materno sobre el nervio ciático en fetos y los efectos protectores de la vitamina E (Vit E) y ácido fólico (AF) en fetos de ratas. Se administraron a ratas ácido valproico (400mg/kg), ácido fólico (400mg/kg) y vitamina E (250 mg/kg) en cada uno de los días de gestación 8-10. Todos los fetos fueron recogidos a los 20 días de gestación. Finas secciones de biopsias obtenidas de los nervios ciáticos de fetos fueron teñidos con acetato de uranilo y examinados bajo microscopio electrónico de transmisión. Los fetos (n: 36) fueron divididos en cinco grupos: control, avp, avp+af, avp+vit e y avp+fa+vit e. En cada grupo, se realizaron los procedimientos farmacológicos, quirúrgicos y los métodos histológicos. Los pesos y longitudes de los fetos de cada grupo fueron comparados y analizados usando la prueba One-Way Anova. La administración de dosis únicas de ácido valproico (400 mg / kg) resultó en la pérdida del peso la longitud entre el control y el grupo apv. Sin embargo, las diferencias en la longitud y el peso entre los otros grupos no fueron significativas. Los hallazgos histopatológicos del grupo control fueron normales. En el grupo avp, se mostró especialmente cambios degenerativos en la mielina que envuelve al nervio periféricamente. Además, predominatemente se encontró en las muestras de este grupo fibras colágenas condensadas y zonas ampliamente desmielinizadas, mientras que las zonas moderadamente afectadas eran relativamente normales. Ambos grupos avp+fa y avp+vit e exhibieron cambios ultraestructurales similares, lo que supone un mínimo o moderado cambio degenerativo. El grupo avp+fa+vit e tuvo casi una estructura normal. La administración de dosis únicas de ácido valproico (400 mg / kg) produjo un efecto sobre el deterioro del nervio ciático a nivel ultraestructural. La administración de la AF y vitamina E tienen un efecto protector, en cierta medida, ...


Subject(s)
Adolescent , Animals , Pregnancy , Rats , Valproic Acid/administration & dosage , Valproic Acid/adverse effects , Valproic Acid/toxicity , Sciatic Nerve/anatomy & histology , Sciatic Nerve , Sciatic Nerve/embryology , Vitamin E/administration & dosage , Vitamin E/therapeutic use , Folic Acid/administration & dosage , Folic Acid/therapeutic use , Pregnancy, Animal , Rats, Wistar/anatomy & histology , Rats, Wistar/embryology
12.
Int. j. morphol ; 27(2): 519-525, June 2009.
Article in English | LILACS | ID: lil-563106

ABSTRACT

Oxygen free radicals are considered to be important components involved in the pathophysiological tissue alterations observed during ischemia-reperfusion (I/R). In this study, we investigated the putative protective effects of melatonin treatment on pancreatic I/R injury. Sprague Dawley male rats were subjected to 30 min of pancreatic pedicle occlusion followed by 90 min reperfusion. Melatonin (10 mg/kg. s.c) was administrated 30 min prior to ischemia or I/R application. At the end of the reperfusion periods, rats were decapitated. Pancreatic samples were taken for transmission electron microscopy. The results indicated that ischemia created b cell damage as evidenced by dilatation between the nucleus inner and outer membrane and degeneration on islets of Langerhans cells, was reversed by melatonin treatment. As melatonin administration reversed these microscopic damage, it seems likely that melatonin protects pancreatic tissue against oxidative damage.


Los radicales libres del oxígeno son considerados como uno de los componentes más importantes que participan en las alteraciones fisiopatológicas del tejido durante la isquemia-reperfusión (I/R). En este estudio, se investigó el supuesto efecto protector del tratamiento de melatonina sobre la lesión pancreática I/R. Ratas Sprague Dawley machos fueron sometidas a 30 minutos de oclusión del pedículo pancreático seguido de 90 minutos de reperfusión. La melatonina (10 mg/kg) fue administrada 30 minutos antes de la isquemia o de la aplicación I/R. Al finalizar los periodos de reperfusión, las ratas fueron decapitadas. Fueron tomadas muestras pancreáticas para el análisis en microscopía electrónica de transmisión. Los resultados indicaron que la isquemia ocasionó daño en las células beta demostrado por la dilatación entre el núcleo interior y la membrana exterior y la degeneración de los islotes de células pancreáticas, los que fueron revertidos por el tratamiento de melatonina. Como la administración de melatonina revirtió estos daños microscópicos, parece probable que ella proteja al tejido pancreático contra el daño oxidativo.


Subject(s)
Male , Animals , Rats , Melatonin/administration & dosage , Melatonin/metabolism , Melatonin/therapeutic use , Pancreas , Pancreas/injuries , Pancreas/metabolism , Pancreas , Reperfusion Injury/drug therapy , Reperfusion Injury/veterinary , B-Lymphocytes , B-Lymphocytes/metabolism , B-Lymphocytes/ultrastructure , Microscopy, Electron, Transmission/methods , Microscopy, Electron, Transmission/veterinary , Rats, Sprague-Dawley/injuries , Rats, Sprague-Dawley/metabolism
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