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Article | IMSEAR | ID: sea-203312


Objectives: To study the correlation between CD4 count &HIV-1 viral load among ART Naive patients attending ICTCSMS Medical College, Jaipur.Material and Methods: This study was conducted on 250 HIVserologically confirmed, ART Naive cases from ICTC, SMSJaipur. RNA extraction was done from plasma samples byQiagen Viral RNA Mini Kit then HIV-1 Viral load wasdetermined by Qiagen HIV-1 viral load kit on ABI 7500 Fast dxReal Time PCR, while CD4 count was done on FACSCALIBUR flowcytometer (BD Biosciences). SPSS ver. 21.0was used to determine correlation between CD4 count & HIV-1viral load.Results: Out of 250, 216 (86.4%) cases were found in whichviral RNA was detected. These samples were correlated withtheir CD4 Count. The mean of viral load was 194746.2791 ±550442.61805 IU/ml while CD4 count was 282.7674 ±217.56456 cells/ul. Females were having Avg. Viral load228506.7273 & CD4 count 337.21 and males were found tohave Avg. Viral load 179791.9866 & CD4 count 258.65Conclusion: This study concluded a negativecorrelation between HIV-1 RNA viral load and CD4 count inHIV-seropositive ART naïve patients of this part of the country.Our study confirmed that HIV-1 RNA viral load levels aresignificantly higher in women than in men, but no suchsignificant gender difference in the CD4 count was found.

Article | IMSEAR | ID: sea-203363


Objectives: To identify cause of HIV seropositive but ViralRNA undetected naive cases in Rajasthan.Materials and Methods: This study included 250 HIV seropositive cases from ICTC Centre, SMS Jaipur .HIV Viral loadwas done by Qiagen HIV-1 viral load kit on ABI 7500 Fast dxReal Time PCR, while CD4 count was done on FACSCALIBUR flowcytometer (BD Biosciences).Results: A total of 250 HIV Serological confirmed positivesamples were tested for HIV-1 RNA viral load & CD Count inthe period of 2014-19. Out of 250, 26 (10.4%) cases werefound in which viral RNA were undetected. out of 26seropositive but HIV RNA undetected case, 16(61.5%) wereMale while 10 (38.5%) were females. Mean of CD4 in Femalewas 565 while 650 in males.Conclusion: HIV‑1 seropositive patients but undetected viralload is not uncommon in society. HIV sero-positive but viralRNA undetected males CD4 count were higher than females. Itwas also observed that all the cases of seropositive withundetected viral load had higher CD4 count comparative withseropositive with viral RNA detected patients. It is concludedthat RNA viral load test is a prognosis test, although suchpatients who had seropositive but viral load undetected needfurther investigations after 3-4 weeks to monitor the status ofthe disease

Indian J Pathol Microbiol ; 2008 Jan-Mar; 51(1): 127-9
Article in English | IMSEAR | ID: sea-75765


CD4 T lymphocyte count is used to measure the progression of HIV infection and to monitor the response to antiretroviral therapy. Information on reference CD4 and CD8 T cell counts in healthy individuals is lacking in northwest India. Samples from 65 HIV-seronegative healthy volunteers (males, 37; females, 28) aged 18 through 59 years were analyzed using FACS (Fluorescent Antibody Cell Sorter) Count TM System. The values of mean and standard deviation of each lymphocyte subpopulation were estimated. The mean +/- SD of absolute numbers of CD4 and CD8 lymphocytes/microl was 743.4 +/- 307.8 and 541.7 +/- 176.4 in males and 790.7 +/- 280.4 and 497.03 +/- 203.6 in females respectively. The range of CD4 counts was 379 to 1800 in males and 321 to 1265 in females. The mean CD4:CD8 ratio was 1.43 +/- 0.56 in males and 1.78 +/- 0.76 in females. The results of this study show a wide variability in CD4 counts in the Indian population. A large multicentric study would define normal ranges of CD4, CD8, and CD4:CD8 ratios among the Indian population.

Adolescent , Adult , CD4 Lymphocyte Count , CD4-CD8 Ratio , CD4-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/immunology , Female , Flow Cytometry , Humans , India , Male , Middle Aged