Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 25
Filter
1.
Braz. j. biol ; 84: e255664, 2024. graf
Article in English | LILACS-Express | MEDLINE, LILACSEXPRESS, LILACS, VETINDEX | ID: biblio-1360227

ABSTRACT

Abstract Magnolia biondii Pamp is an important ornamental tree species widely grown and used as a rootstock in the propagation of different Magnolia varieties. In the current studies, anatomical, physiological and endogenous hormones were studied to check the effect of IBA 750 mg/L on the adventitious rooting and to provide theoretical and technical support for the propagation of Magnolia biondii Pamp through stem cuttings. Two thousand stem cuttings were prepared and divided into two groups i.e., IBA treated cuttings and water control. For the evaluation of antioxidant enzyme activities, and endogenous hormones levels, samples were collected on the day of planting and each 5th day and further steps were carried out in the laboratory according to the protocols and proper precautions. For the anatomical observations, samples were collected on the 13th, 15th, and 17th day for IBA treated cuttings while 21st, 23rd, and 25th day for control. Collected samples were preserved in the FAA solution and further observations were carried out in the laboratory. Anatomical observations showed that it took 13 days for the differentiation of root primordia to the appearance of young adventitious roots in IBA treated cuttings, while it took 21 days to develop primordia in the control. Antioxidant enzyme activities involved in ROS were significantly higher in the IBA treated cuttings compared to control. POD showed a peak on the 13th day before the emergence of roots in IBA treated cuttings while it showed a peak on the 21st day in the control. PPO showed a peak on the 21st day in the IBA treated cuttings while it showed a peak on the 29th day in the control. SOD showed a peak on the 17th day in IBA treated cuttings, while it showed a peak on the 25th day in the control. Exogenous application of IBA enhanced the endogenous IAA and GA3 levels compared to CK, while it reduced the levels of ABA continuously at the time of rooting and then increased gradually. Inclusively, our study suggests that IBA 750 mg/L is efficient for the rooting of Magnolia biondii Pamp cuttings, as it enhanced the process of antioxidant enzyme activities, endogenous hormones levels and reduced the time of root formation which is evident from the anatomical observations.


Resumo Magnolia biondii Pamp é uma importante espécie de árvore ornamental muito cultivada e utilizada como porta-enxerto na propagação de diferentes variedades de Magnolia. Nos estudos atuais, hormônios anatômicos, fisiológicos e endógenos foram estudados para verificar o efeito do AIB na dose de 750 mg / L no enraizamento adventício e fornecer suporte teórico e técnico para a propagação de M. biondii Pamp por meio de estacas. Duas mil estacas foram preparadas e divididas em dois grupos, ou seja, tratadas com AIB e controle de água. Para a avaliação das atividades das enzimas antioxidantes e dos níveis de hormônios endógenos, as amostras foram coletadas no dia do plantio e a cada 5 dias, enquanto as demais etapas foram realizadas em laboratório de acordo com os protocolos e os devidos cuidados. Para as observações anatômicas, as amostras foram coletadas no 13º, 15º e 17º dias para estacas tratadas com AIB e no 21º, 23º e 25º dias para o controle. As amostras coletadas foram preservadas em solução FAA, e outras observações foram realizadas em laboratório. Observações anatômicas mostraram a necessidade de 13 dias para a diferenciação dos primórdios radiculares até o aparecimento de raízes adventícias jovens em estacas tratadas com AIB e de 21 dias para o desenvolvimento dos primórdios no controle. As atividades das enzimas antioxidantes envolvidas nas ROS foram significativamente maiores nas estacas tratadas com AIB em comparação com o controle. A POD apresentou pico no 13º dia antes da emergência das raízes nas estacas tratadas com AIB, enquanto no 21º dia apresentou pico no controle. A PPO teve pico no 21º dia nas estacas tratadas com AIB e no 29º dia no controle. A SOD apresentou pico no 17º dia nas estacas tratadas com AIB e no 25º dia no controle. A aplicação exógena de AIB aumentou os níveis endógenos de IAA e GA3 em relação ao controle, enquanto reduziu os níveis de ABA continuamente no momento do enraizamento e, em seguida, aumentou gradativamente. Inclusive, nosso estudo sugere que o AIB na dose de 750 mg / L é eficiente para o enraizamento de estacas de M. biondii Pamp, visto que potencializou o processo de atividades de enzimas antioxidantes e os níveis de hormônios endógenos, além de reduzir o tempo de formação de raízes, o que fica evidente nas observações anatômicas.

2.
Rev. Assoc. Med. Bras. (1992) ; 68(2): 217-221, Feb. 2022. tab
Article in English | LILACS | ID: biblio-1365360

ABSTRACT

SUMMARY OBJECTIVE: This study aimed at the oral health problems of elderly patients with diabetes. A training course of integrated traditional Chinese and Western medicine was constructed, helping patients improve their oral health quality of life. METHODS: A randomized controlled prospective experimental study was conducted. A total of 190 elderly patients were divided randomly into an observation group and a control group with 95 cases in each. The control group received regular health education, while the observation group was based on the control group to implement the integrated experiential learning of traditional Chinese and Western medicine in small groups. The oral health knowledge, attitude, behavior, and blood glucose control status along with the oral health quality of life of the two groups were compared before the intervention and at 3-month postintervention. RESULTS: Three months after the intervention, the fasting blood glucose control and the 2-h postprandial blood glucose/glycosylated hemoglobin levels in the observation group were significantly better than in the control group, and the difference was statistically significant (p<0.05). The oral health quality of life in the observation group was significantly better than in the control group, and the difference was statistically significant (p<0.05). CONCLUSION: The small-group experiential learning model of integrated Chinese and Western medicine can promote the transformation of knowledge-beliefs-behaviors in elderly patients with diabetes, which is conducive to controlling blood sugar levels and improving the quality of oral health.


Subject(s)
Humans , Aged , Oral Health , Diabetes Mellitus/therapy , Quality of Life , China , Prospective Studies , Problem-Based Learning , Medicine, Chinese Traditional
3.
Braz. j. med. biol. res ; 54(7): e10612, 2021. tab, graf
Article in English | LILACS | ID: biblio-1249314

ABSTRACT

Genomic studies have provided insights into molecular subgroups and oncogenic drivers of pediatric brain tumors (PBT) that may lead to novel therapeutic strategies. Participants of the cohort Pediatric Brain Tumor Atlas: CBTTC (CBTTC cohort), were randomly divided into training and validation cohorts. In the training cohort, Kaplan-Meier analysis and univariate Cox regression model were applied to preliminary screening of prognostic genes. The LASSO Cox regression model was implemented to build a multi-gene signature, which was then validated in the validation and CBTTC cohorts through Kaplan-Meier, Cox, and receiver operating characteristic curve (ROC) analyses. Also, gene set enrichment analysis (GSEA) and immune infiltrating analyses were conducted to understand function annotation and the role of the signature in the tumor microenvironment. An eight-gene signature was built, which was examined by Kaplan-Meier analysis, revealing that a significant overall survival difference was seen, either in the training or validation cohorts. The eight-gene signature was further proven to be independent of other clinic-pathologic parameters via the Cox regression analyses. Moreover, ROC analysis demonstrated that this signature owned a better predictive power of PBT prognosis. Furthermore, GSEA and immune infiltrating analyses showed that the signature had close interactions with immune-related pathways and was closely related to CD8 T cells and monocytes in the tumor environment. Identifying the eight-gene signature (CBX7, JADE2, IGF2BP3, OR2W6P, PRAME, TICRR, KIF4A, and PIMREG) could accurately identify patients' prognosis and the signature had close interactions with the immunodominant tumor environment, which may provide insight into personalized prognosis prediction and new therapies for PBT patients.


Subject(s)
Humans , Child , Brain Neoplasms/genetics , Gene Expression Profiling , Prognosis , Gene Expression Regulation, Neoplastic , Cell Cycle Proteins , Kaplan-Meier Estimate , Tumor Microenvironment , Polycomb Repressive Complex 1
4.
Electron. j. biotechnol ; 43: 16-22, Jan. 2020. tab, graf
Article in English | LILACS | ID: biblio-1087512

ABSTRACT

Background: The intestinal bacterial community has an important role in maintaining human health. Dysbiosis is a key inducer of many chronic diseases including obesity and diabetes. Kunming mice are frequently used as a model of human disease and yet little is known about the bacterial microbiome resident to the gastrointestinal tract. Results: We undertook metagenomic sequencing of the luminal contents of the stomach, duodenum, jejunum, ileum, cecum, colon, and rectum of Kunming mice. Firmicutes was the dominant bacterial phylum of each intestinal tract and Lactobacillus the dominant genus. However, the bacterial composition differed among the seven intestinal tracts of Kunming mice. Compared with the small intestine, the large intestine bacterial community of Kunming mice is more stable and diverse. Conclusions: To our knowledge, ours is the first study to systematically describe the gastrointestinal bacterial composition of Kunming mice. Our findings provide a better understanding of the bacterial composition of Kunming mice and serves as a foundation for the study of precision medicine.


Subject(s)
Animals , Mice , Bacteria/isolation & purification , Gastrointestinal Tract/microbiology , Bacteria/genetics , RNA, Ribosomal, 16S , Polymerase Chain Reaction , High-Throughput Nucleotide Sequencing , Firmicutes/isolation & purification , Gastrointestinal Microbiome , Lactobacillus/isolation & purification
5.
Braz. j. med. biol. res ; 53(1): e8669, Jan. 2020. tab, graf
Article in English | LILACS | ID: biblio-1055478

ABSTRACT

This study aimed to investigate the therapeutic effect of fasudil on treating experimental autoimmune neuritis (EAN). Twenty-four EAN mice were randomly assigned to fasudil treatment (Fasudil group) or saline treatment (EAN model group) for 28 days. Clinical symptom score was evaluated every other day; inflammatory cell infiltration, demyelination, anti-myelin basic protein (MBP), inflammatory cytokines, inducible nitric oxide synthase (iNOS), and arginase-1 were detected in sciatic nerves at day 28. Th1, Th2, Th17, and Tregs proportions in splenocytes were detected at day 28. Clinical symptom score was found to be attenuated in the Fasudil group compared to the EAN model group from day 12 to day 28. Sciatic nerve inflammatory cell counts by HE staining and demyelination by luxol fast blue staining were both reduced, while MBP was increased in the Fasudil group compared to the EAN model group at day 28. Interferon γ (IFN-γ) and interleukin (IL)-17 were reduced, while IL-4 and IL-10 were elevated in the Fasudil group at day 28. Sciatic nerve M1 macrophages marker iNOS was decreased while M2 macrophages marker arginase-1 was increased in the Fasudil group at day 28. CD4+IFN-γ+ (Th1) and CD4+IL-17+ (Th17) cell proportions were both decreased, CD4+IL-4+ (Th2) cell proportion was similar, while CD25+FOXP3+ (Treg) cell proportion in splenocytes was increased in the Fasudil group. In summary, fasudil presented a good therapeutic effect for treating EAN by attenuating Th1/Th17 cells and promoting Tregs activation as well as M2 macrophages polarization.


Subject(s)
Animals , Female , Rabbits , Interleukins/blood , Interferon-gamma/blood , T-Lymphocytes, Helper-Inducer/drug effects , Neuritis, Autoimmune, Experimental/drug therapy , Sciatic Nerve/drug effects , Sciatic Nerve/metabolism , Time Factors , Real-Time Polymerase Chain Reaction , RNA, Mitochondrial , Mice, Inbred C57BL , Neuritis, Autoimmune, Experimental/blood
6.
Organ Transplantation ; (6): 605-2020.
Article in Chinese | WPRIM | ID: wpr-825579

ABSTRACT

Objective To evaluate the clinical value of lymphocyte subset classification in the diagnosis of active pulmonary tuberculosis in renal transplant recipients. Methods Clinical data of 52 recipients undergoing renal transplantation were retrospectively analyzed. According to the results of imaging and etiological examination, 52 recipients were divided into the stable group(n=19), tuberculosis group (n=9), bacteria group (n=12) and fungi group (n=12), respectively. The renal function of recipients was compared, and the proportion and absolute value of lymphocyte subset were analyzed and compared among four groups. The diagnostic value of lymphocyte subset classification for active pulmonary tuberculosis after renal transplantation was evaluated. Results Compared with the stable group, the levels of blood urea nitrogen and serum creatinine in the tuberculosis group, bacteria group and fungi group were significantly increased (all P < 0.05). The proportion of CD3+, CD8+, CD4+, natural killer (NK) cells and CD19+ lymphocyte subsets were not significantly different (all P>0.05). And the absolute values of CD3+, CD8+, CD4+, NK cells and CD19+ lymphocyte subsets were significantly decreased (all P < 0.05). The proportion of CD8+ lymphocyte subset in the tuberculosis group and fungi group was significantly higher than that in the bacteria group (both P < 0.05). The optimal cut-off value of CD8+ lymphocyte subset ratio in the differential diagnosis of active pulmonary tuberculosis and bacterial pneumonia was 33.27%, and the sensitivity and specificity were 0.889 and 0.833, respectively. The area under the curve (AUC) was 0.880. Conclusions The classification of lymphocyte subset can provide auxiliary diagnostic basis for differential diagnosis and individualized treatment of active pulmonary tuberculosis and bacterial pneumonia in renal transplant recipients.

7.
Article in Chinese | WPRIM | ID: wpr-825120

ABSTRACT

@#[Abstract] Objective: To observe the effects of shikonin on the proliferation, apoptosis and cell cycle of human esophageal carcinoma TE-1 cells, and to explore its mechanism. Methods: TE-1 cells were treated with different concentrations of shikonin (0, 1, 5, 10 µmol/L). MTT assay was used to detect cell proliferation at different time points (24, 48 and 72 h). After treatment with shikonin for 48 h, cell apoptosis in TE-1 cells of each group was observed with Hoechst 33258 fluorescence staining. Flow cytometry was used to detect apoptosis and cell cycle. The changes in expression of TRAP1/Akt/mTOR signaling pathway related proteins were detected by Western blotting. Results: Shikonin inhibited the proliferation of TE-1 cells in a time-dose-dependent manner (P<0.05 or P<0.01). Compared with the control group, shikonin significantly promoted the apoptosis of TE-1 cells (P<0.01), induced the G0/G1 phase block of TE-1 cells (P<0.05 or P<0.01), and reduced the expression levels of TRAP1, p-Akt and p-MTOR (P<0.05 or P<0.01). The above effects were all dose-dependent. Conclusion: Shikonin can significantly inhibit the proliferation of TE-1 cells in vitro, induce G0/G1 phase arrest and promote apoptosis, which may be closely related to the inhibition of TRAP1/Akt/mTOR signaling pathway.

8.
Article in Chinese | WPRIM | ID: wpr-825026

ABSTRACT

Objective@#To analyze the characteristics of perioperative mortality (POM) in oral maxillofacial head and neck cancer patients.@*Methods@#A retrospective study was adapted for head and neck cancer patients who were treated and had POM in the department of oral maxillofacial & head and neck oncology in Shanghai Ninth People′s Hospital from Jan 1999 to Dec 2019. Demographic information, disease characteristic and clinical records were collected. The factors of POM were classified into surgical complication, medical complication, mixed complication (surgical and medical) and personal complication. SPSS 17.0 software was used to analyze the cause composition for POM under different condition.@*Results @# 55 patients were included: 39 were male, and 16 were female. A total of 12 patients had a smoking history. Furthermore, 28 patients had general comorbidities. 20 underwent preoperative radiotherapy and 9 received preoperative chemotherapy. Squamous cell carcinoma was the most frequent pathological diagnosis in 37 patients. A total of 9 patients had tumors in the maxilla and skull base. In addtition, 4 patients had POM preoperatively, 1 patient had POM within the operation, and 50 patients had POM postoperatively. The leading causes of death were as follows: rupture of the carotid artery was the most frequent (8), and the surgical complication of pulmonary infection was the main medical complication (6). Pulmonary infection and hemorrhage were regarded as the main mixed complication (4). Two patients had POM due to personal complications. The higher the American Society of Anesthesiologists (ASA) score, the higher the proportion of medical factors in POM (P=0.039).@*Conclusion@#The composition of POM in oral maxillofacial- head and neck cancer (OMHNC) patients was complicated. Carotid artery rupture was the most common and fatal surgical complication, especially for those who underwent preoperative radiotherapy. Pulmonary infection was the most frequent medical complication, and those who had a higher ASA grade tended to have more complication.

9.
Chinese Journal of School Health ; (12): 854-857, 2019.
Article in Chinese | WPRIM | ID: wpr-818669

ABSTRACT

Objective@#To investigate the relationships between behavioral problems and family environment in obese children aged 6-15 years to provide a dereference for the analysis of behavioral problems among obese children and the construction of their family environmet .@*Methods@#Family environment dimensions and behavioral problems were investigated among 2 376 obese children and 2 376 non-obese children with Family Environment Scale Symptoms Questionnaire and Achenbach Child Behavior Checklist. Data were analyzed by SPSS 17.0.@*Results@#The incidence of the behavioral problems in obese children (21.12%) was higher than that of non-obese children (15.53%)(χ2=24.93,P<0.01).The scores of Family Environment Scale Symptoms Questionnaire differed between obese children and nonobese children (Z=-2.44, -2.98, -3.38, P<0.05). Binary logistic regression results showed that for obese boys and girls aged 6 to 11 years, high maternal education levels(OR=0.78, 95%CI=0.69-0.88), family cohesion(OR=0.75, 95%CI=0.67-0.84) and less family conflicts(OR=0.81, 95%CI=0.75-0.87) were related to less behavioral problems; for obese boys and girls aged 12 to 15 years, family cohesion(OR=0.79, 95%CI=0.70-0.90) and less family conflicts(OR=0.78, 95%CI=0.71-0.86) were related to less behavioral problems.@*Conclusion@#Family cohesion and less family conflicts might help low the risk of behavioral problems among obese children. The role of family environment on behavioral problems should not be neglected in future mental health promotion and social work service for obese children.

10.
Braz. j. microbiol ; 49(3): 552-558, July-Sept. 2018. tab, graf
Article in English | LILACS | ID: biblio-951796

ABSTRACT

Abstract Surveillances and interventions on antibiotics use have been suggested to improve serious drug-resistance worldwide. Since 2007, our hospital have proposed many measures for regulating surgical prophylactic antibiotics (carbapenems, third gen. cephalosporins, vancomycin, etc.) prescribing practices, like formulary restriction or replacement for surgical prophylactic antibiotics and timely feedback. To assess the impacts on drug-resistance after interventions, we enrolled infected patients in 2006 (pre-intervention period) and 2014 (post-intervention period) in a tertiary hospital in Shanghai. Proportions of targeted pathogens were analyzed: methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus spp. (VRE), imipenem-resistant Escherichia coli (IREC), imipenem-resistant Klebsiella pneumoniae (IRKP), imipenem-resistant Acinetobacter baumannii (IRAB) and imipenem-resistant Pseudomonas aeruginosa (IRPA) isolates. Rates of them were estimated and compared between Surgical Department, ICU and Internal Department during two periods. The total proportions of targeted isolates in Surgical Department (62.44%, 2006; 64.09%, 2014) were more than those in ICU (46.13%, 2006; 50.99%, 2014) and in Internal Department (44.54%, 2006; 51.20%, 2014). Only MRSA has decreased significantly (80.48%, 2006; 55.97%, 2014) (p < 0.0001). The percentages of VRE and IREC in 3 departments were all <15%, and the slightest change were also both observed in Surgical Department (VRE: 0.76%, 2006; 2.03%, 2014) (IREC: 2.69%, 2006; 2.63%, 2014). The interventions on surgical prophylactic antibiotics can be effective for improving resistance; antimicrobial stewardship must be combined with infection control practices.


Subject(s)
Humans , Postoperative Complications/microbiology , Bacteria/drug effects , Bacterial Infections/microbiology , Cross Infection/microbiology , Anti-Bacterial Agents/administration & dosage , Postoperative Complications/prevention & control , Bacteria/isolation & purification , Bacteria/growth & development , Bacterial Infections/prevention & control , Preoperative Care , Drug Resistance , Microbial Sensitivity Tests , China , Cross Infection/prevention & control , Antibiotic Prophylaxis
11.
Indian J Dermatol Venereol Leprol ; 2018 May; 84(3): 269-274
Article | IMSEAR | ID: sea-192368

ABSTRACT

Background: Vitiligo is a disorder caused by the loss of the melanocyte activity on melanin pigment generation. Studies show that oxidative-stress induced apoptosis in melanocytes is closely related to the pathogenesis of vitiligo. Glutamine is a well known antioxidant with anti-apoptotic effects, and is used in a variety of diseases. However, it is unclear whether glutamine has an antioxidant or anti-apoptotic effect on melanocytes. Aims: The aim of this study was to investigate the protective effects of glutamine on a human melanocyte oxidative stress model. Methods: The oxidative stress model was established on human melanocytes using hydrogen peroxide. The morphology and viability of melanocytes, levels of oxidants [reactive oxygen species and malondialdehyde], levels of antioxidants [superoxide dismutase and glutathione-S-transferase], and apoptosis-related indicators (caspase-3, bax and bcl-2) were examined after glutamine exposure at various concentrations. Expressions of nuclear factor-E2-related factor 2, heme oxygenase-1, and heat shock protein 70 were detected using western blot technique after glutamine exposure at various concentrations. Results: Our results demonstrate that pre-treatment and post-treatment with glutamine promoted melanocyte viability, increased levels of superoxide dismutase, glutathione-S-transferase and bcl-2, decreased levels of reactive oxygen species, malondialdehyde, bax and caspase-3, and enhanced nuclear factor-E2-related factor 2, heme oxygenase-1, and heat shock protein 70 expression in a dose dependent manner. The effect of pre-treatment was more significant than post-treatment, at the same concentration. Limitations: The mechanisms of glutamine activated nuclear factor-E2-related factor 2 antioxidant responsive element signaling pathway need further investigation. Conclusions: Glutamine enhances the antioxidant and anti-apoptotic capabilities of melanocytes and protects them against oxidative stress.

12.
Article in Chinese | WPRIM | ID: wpr-333466

ABSTRACT

This study evaluated the effects of sodium hypochlorite (NaOC1) with different concentrations and exposure time on the structural,compositional and mechanical properties of human dentin in vitro.Sixty dentin slabs were obtained from freshly extracted premolars,randomly distributed into four groups (n=15),and treated with 1%,5%,10% NaOC1 and distilled water (control group),respectively,for a total of 60 min.Attenuated total reflection infrared (ATR-IR) spectroscopy,Raman spectroscopy and X-ray diffraction (XRD) were carried out before,10 min and 60 min after the treatment.Scanning electron microscopy (SEM) and flexural strength test were conducted as well.The results showed that dentins experienced morphological alterations in the NaOC1 groups,but not in the control group.Two-way repeated-measures analysis of variance revealed that the carbonate:mineral ratio (C:M),Raman relative intensity (RRI),a-axis,c-axis length and full width at half maximum (FWHM) with the increase of time and concentration in the NaOC1 groups were not significantly different from those in the control group (P>0.05).Nevertheless,the mineral:matrix ratio (M:M) increased and the flexural strength declined with the increase of concentration and the extension of time in the NaOC1 groups (P<0.05).Additionally,it was found that the M:M and the flexural strength remained unchanged after 1% NaOCl treatment (P>0.05),and the morphology changes were unnoticeable within 10 min in 1% NaOC1 group.These results indicated that NaOC1 has no significant effects on the inorganic mineral of human dentin;but it undermines and eliminates the organic content concentration-and time-dependently,which in turn influences the flexural strength and toughness of dentins.In addition,an irrigation of 1%NaOCl within 10 min can minimize the effects of NaOC1 on the structural and mechanical properties of dentin during root canal treatment.

13.
Article in Chinese | WPRIM | ID: wpr-333427

ABSTRACT

The effect of high-frequency repetitive transcranial magnetic stimulation (rTMS) on potassium-chloride cotransporter-2 (KCC2) protein expression following spinal cord injury (SCI) and the action mechanism were investigated.SCI models were established in SD rats.Five groups were set up randomly:normal control group,SCI 7-day (7D) model group,SCI 14-day (14D) model group,SCI-7D rTMS group and SCI-14D rTMS group (n=5 each).The rats in SCI rTMS groups were treated with 10 Hz rTMS from 8th day and 15th day after SCI respectively,once every day,5 days every week,a total of 4 weeks.After the model establishment,motor recovery and spasticity alleviation were evaluated with BBB scale once a week till the end of treatment.Finally,different parts of tissues were dissected out for detection of variations of KCC2 protein using Western blotting and polymerase chain reaction (PCR) technique.The results showed that the BBS scores after treatment were significantly higher in SCI-7D rTMS group than in SCI-14D rTMS group (P<0.05).As compared with normal control groups,The KCC2 protein in SCI model groups was down-regulated after SCI,and the decrease was much more significant in SCI-14D model group than in SCI-7D group (P<0.05).As compared with SCI model groups,KCC2 protein in rTMS groups was up-regulated after the treatment (P<0.05).The up-regulation of KCC2 protein content and expression was more obvious in SCI-7D rTMS group than in SCI-14D rTMS group (P<0.05).It was concluded that 10 Hz rTMS can alleviate spasticity in rats with SCI,which might be attributed to the up-regulation of KCC2 protein.It was also suggested that the high-frequency rTMS treatment after SCI at early stage might achieve more satisfactory curative effectiveness.

14.
International Eye Science ; (12): 2263-2269, 2017.
Article in Chinese | WPRIM | ID: wpr-669405

ABSTRACT

·Retinal angiomatous proliferation ( RAP) , also known as"type 3 neovascularization", is a well - recognized variation of neovascular age - related macular degeneration ( nARMD ) . Neovascularization is the basic pathological characteristic. Current view on the origin of the neovascularization is the deep retinal capillaries. The main clinical features include retinal pigment epithelium detachment( PED) and reticular pseudodrusen. These two features have close relation to the retinal pigment epithelium ( RPE ) tear and geographic atrophy ( GA ) , respectively, which may ultimately result in severe irreversible visual impairment. The disease has a rapid natural course and the majority of patients in early stage can develop into vision loss within 6mo. However, classical therapeutic managements, such as laser therapy, have limited efficacy and poor prognosis. Recently, RAP has been further understood with the application of OCT angiography and other new technologies in diagnosing, staging and monitoring RAP. Varieties of research on intravitreal injection of anti -vascular endothelial growth factor ( VEGF ) treatment to RAP have also revealed its promising results and proved its safety as well as effectiveness. All these have provided new knowledge on choosing the optimal treatment regimen in clinical.

15.
Article in Chinese | WPRIM | ID: wpr-751126

ABSTRACT

@#Objective    To explore the safety, feasibility and learning curve of video-assisted thoracoscopic surgery(VATS) in treatment of thoracic diseases. Method    We retrospectively analyzed the clinical data of 591 patients of thoracic surgery in our hospital between September 2009 and September 2016. There were 378 males and 213 females at age of 14–82 years. Result    All patients were successfully completed surgery. Twelve patients converted to open chest with conversion rate of 2.0%. Postoperative complications occurred in 24 patients (4.1%). Four patients died during the perioperative period, and mortality rate was 0.7%. The learning curve of VATS for lung cancer was about 25 patients. And the learning curve of video-assisted laparoscopy for resection of esophageal cancer was about 15 patients. Conclusion    VATS is safety and feasible for the chest disease patients in municipal hospital, and is worthy to popularize.

16.
Article in Chinese | WPRIM | ID: wpr-750310

ABSTRACT

@#Objective    To compare the subaxillary small incision thoracotomy (SSIT) with video-assisted thoracic surgery (VATS) for patients with lung cancer. Methods    Retrospective analysis of 142 patients with lung cancer in Department of Thoracic Surgery, The First People's Hospital of Neijiang from January 2014 to April 2016 was conducted. There were 86 males and 56 females, aged 40-77 years. Patients were divided into a VATS group (n=72) and a SSIT group (n=70). The following postoperative data were evaluated: operation time, number of dissected lymph nodes, intraoperative bleeding, postoperative chest drainage volume, drainage duration, postoperative ambulation time, average hospital stay, postoperative complications, hospitalization cost, early postoperative incision pain (visual analogue scale, VAS) and other indicators. Results    There were no statistically significant differences between the two groups in the operation time (120.8±20.4 min vs. 126.2±21.6 min, P=0.124), the dissected lymph node (11.1±2.0 vs. 11.4±1.9, P=0.333) and the postoperative complications rate (13.9% vs. 15.7%, P=0.759). Laparoscopic intraoperative bleeding and postoperative drainage volume were significantly less in the VATS group than those in the SSIT group (123.2±26.9 ml vs. 156.4±24.0 ml, P<0.001; 227.0±75.5 ml vs. 334.3±89.1 ml, P<0.001). Postoperative drainage duration, postoperative ambulation time and hospital stay were shorter in the VATS group than those in the SSIT group (2.5±0.5 d vs. 3.1±0.6 d, 1.5±0.5 d vs. 2.2±0.6 d, 6.5±0.5 d vs. 7.4±0.6 d, all P<0.001). The average hospitalization cost of the VATS group was significantly higher than that of the SSIT group (42 338.9±8 855.7 yuan vs. 32 043.7±7 178.1 yuan, P<0.001). There was no significant difference in the operation cost and anesthesia cost between the two groups (P>0.05). The early postoperative pain of laparoscopic group was less, but the difference was not statistically significant (P>0.05). Conclusion    The hospitalization cost of the SSIT is lower than that of thoracic surgery, which may be beneficial to the appilication in primary hospitals.

17.
International Eye Science ; (12): 2059-2062, 2016.
Article in Chinese | WPRIM | ID: wpr-638124

ABSTRACT

Central serous chorioretinopathy( CSC) is an important cause for central vision loss. It mostly occurs in young and middle-aged men. It is a condition characterized by a serous detachment of neurosensory retina at the posterior pole and leakage from the retinal pigment epithelium ( RPE ) . Most patients with acute CSC will resolve spontaneously. However, in cases of chronic CSC with persistent serous retinal detachment, patients might develop progressive vision loss. This article was made a brief review of the current treatment methods, including laser, photodynamic therapy ( PDT ) , intravitreal anti-vascular endothelial growth factor therapy and the mineralocorticoid receptor antagonists.

18.
Pakistan Journal of Pharmaceutical Sciences. 2015; 28 (6): 2173-2178
in English | IMEMR | ID: emr-174531

ABSTRACT

The aim of the present work was to prepare a nasal spray of bisoprolol fumarate [BF]. The Pharmacokinetics and relative bioavailability of the BF nasal formulation were evaluated in Wistar rats. The BF nasal spray after administration exhibited very fast absorption and higher plasma drug concentration. The maximum plasma concentration [C[max]] and the time to reach it [T[max]] were 409.5ng/ml and 3.6 min for the BF nasal spray, 39.4ng/ml and 26.7min for the drug solution, respectively. The bioavailability of the BF nasal spray was greater than 1500.0%. Meantime, the effect of the BF nasal spray on nasal mucociliary movement was also studied with a toad palate model. The BF nasal preparation showed minor ciliotoxicity, but the adverse effect was temporary and reversible

19.
Int. j. morphol ; 31(3): 980-985, set. 2013. ilus, tab
Article in English | LILACS | ID: lil-694989

ABSTRACT

This work aims to investigate the effect of fetal amnion-wrapped acellular allogenic nerve transplantation on peripheral nerve injury (PNI) in dogs and to explore its advantages and feasibility in PNI repair. A total of 15 dogs were divided into three groups: the allogenic nerve transplantation (A), amnion-wrapped allogenic nerve transplantation (B), and allogenic nerve donor (C) groups. Neurite counts after myelin and H-E stainings, soleus muscle action potentials, and sciatic nerve conductive velocities were compared between the A and B groups at 16 w after operation. The B group showed better nerve regeneration than the A group at 16 w. Compared with the A group, the B group showed a better growth continuity of the transplanted nerve and milder inflammatory reactions around the nerve. The B group presented much more proliferated Schwannocytes and regenerated nerve fibers than the A group. The neurite density and the amplitude of the soleus muscle action potentials in the B group were significantly higher than those in the A group (P < 0.05). The two groups did not show significant differences in nerve conductive velocities (P > 0.05). Amnion-wrapped acellular allogenic nerve transplantation can improve defected nerve morphology and the quality of transplanted nerve regeneration.


El objetivo fue investigar el efecto del trasplante alogénico de nervio acelular envuelto en membrana amniótica fetal sobre la lesión del nervio periférico (LNP) en perros, y explorar sus ventajas y viabilidad en la reparación de LNP. Quince 15 perros se dividieron en tres grupos: grupo trasplante alogénico de nervio (A), grupo trasplante alogénico de nervio envuelto en membrana amniótica (B), y grupo donante alogénico de nervio (C). Se compararon el recuento de neuritas posterior a la tinción de hematoxilina-eosina (HE) y para mielina, potenciales de acción del músculo sóleo, y velocidades conductoras nerviosas del nervio ciático entre los grupos A y B, 16 semanas después de la operación. El grupo B mostró una mejor regeneración de los nervios que el grupo A a las 16 semanas. En comparación con el grupo A, el grupo B mostró una mejor continuidad del crecimiento del nervio trasplantado con reacciones inflamatorias leves alrededor del nervio. El grupo B presentó fibras nerviosas donde proliferaron más los Schwannocitos y regeneración que el grupo A. La densidad de las neuritas y la amplitud de los potenciales de acción del músculo sóleo en el grupo B fueron significativamente más altos (p <0,05). Ambos grupos no mostraron diferencias significativas en las velocidades conductoras nerviosas (P> 0,05). El trasplante alogénico de nervio acelular envuelto en membrana amniótica puede mejorar la morfología del nervio lesionado y la calidad de regeneración del nervio trasplantado.


Subject(s)
Animals , Dogs , Amnion , Nerve Regeneration , Sciatic Nerve/transplantation , Peripheral Nerve Injuries/surgery , Peripheral Nerve Injuries/pathology , Transplantation, Homologous
20.
Article in English | WPRIM | ID: wpr-299381

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the efficacy and safety of low molecular weight heparin (LMWH) prophylaxis for venous thromboembolism (VTE) after lumbar decompression surgery.</p><p><b>METHODS</b>Patients at high or the highest risk of VTE who underwent lumbar spine surgery in Peking Union Medical College Hospital from January 2004 to April 2011 were included in the present study. All the patients received a half dose of LMWH 6 hours after surgery followed by a full dose LMWH once per day until discharge. We recorded incidences of deep venous thrombosis (DVT), pulmonary embolism (PE), bleeding complications, and medication side effects.</p><p><b>RESULTS</b>Seventy-eight consecutive patients were eligible and enrolled in this study. The mean hospital stat was 8.5±4.5 days. No symptomatic DVT, PE, or major bleeding events were observed. One patient developed wound ecchymosis, another developed wound bleeding, four had mild hepatic aminotransferase level elevation, and one developed a suspicious allergic reaction.</p><p><b>CONCLUSION</b>LMWH may be applied as an effective and safe prophylaxis for VTE in high-risk patients undergoing lumbar decompression surgery.</p>


Subject(s)
Aged , Decompression, Surgical , Female , Heparin, Low-Molecular-Weight , Therapeutic Uses , Humans , Lumbar Vertebrae , General Surgery , Male , Middle Aged , Venous Thromboembolism
SELECTION OF CITATIONS
SEARCH DETAIL