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1.
Article in Chinese | WPRIM | ID: wpr-871161

ABSTRACT

Objective:To test the effectiveness of electrical stimulation of the median nerve (MNES) for relieving post-stroke cognitive impairment (PSCI) and explore the possible mechanism.Methods:Thirty patients with PSCI were randomly divided into a routine treatment group (the control group) and an MNES group, each of 15. Both groups were given routine rehabilitation treatment, including cognitive rehabilitation training, medications and acupuncture. The MNES group additionally received 30 minutes of MNES on their right hands every day, five times a week for six weeks. One electrode was positioned over the median nerve 2cm up from the rasceta of the right wrist. The other was on the muscles of the thenar eminence. Forty seconds of stimulation were applied with intervals of 20 seconds, for 30 min daily. Before and after 3 and 6 weeks of treatment, both groups were evaluated using the mini-mental state examination (MMSE), the Montreal cognitive assessment (MoCA), the modified Barthel index (MBI) and the Fugl-Meyer assessment (FMA). In another 15 patients oxyhemoglobin levels in the brain before and during the MNES were observed using near-infrared spectroscopy.Results:After 3 weeks of treatment, a significant improvement was observed in the average MMSE, FMA and MBI scores of both groups, and the average MoCA score of the observation group. Three weeks later, the average MMSE, FMA, MBI and MoCA scores of both groups had improved significantly compared with before the treatment, with the average MMSE and MoCA improvements in the MNES group significantly greater than the control group′s averages. After 6 weeks of treatment the significant improvements persisted in both groups. Both group′s average FMA scores had also improved significantly, as had the average MBI score of the control group. After 6 weeks of treatment, the observation group′s average time orientation, location orientation, language instant memory, attention, calculation and short-term memory in MMSE had all improved significantly along with visual space capacity, executive capacity, attention, language, orientation and memory in MoCA. The spectroscopic results showed significantly improved oxyhemoglobin concentration in the bilateral frontal lobes after the MNES.Conclusions:Electrical stimulation of the median nerve can help to improve cognition after a stroke. It increases oxyhemoglobin concentration in the bilateral frontal lobes.

2.
Chinese Journal of Orthopaedics ; (12): 1397-1408, 2020.
Article in Chinese | WPRIM | ID: wpr-869093

ABSTRACT

Objective:Compared with closed reduction and percutaneous pinning (CRPP) treatment, evaluating the clinical observation of utilizing a transolecranon pin joystick technique combined with CRPP in the teatment of multidirectionally unstable supracondylar humeral fractures in children.Methods:From thirty nine pediatric multidirectionally unstable supracondylar humeral fractures hospitalized between January 2012 and January 2019, twenty seven males (69.23%) and twelve females (30.77%) were included in the study, with a average age of 6.68±2.52 years (range, 2.17-13.75 y), twenty three fractures (65.7%) were treated with CRPP (CRPP group) and the remaining Sixteen fractures (41.03%) were treated utilizing a transolecranon pin joystick technique combined with CRPP (joystick group). Both groups were followed over 16 weeks. The paired sample t test or χ2 test and Fisher's exact test were used to compare the surgical time, times of fluoroscopy, quality of reduction and neurological or vascular complications, Baumann angle, carrying angle, lateralcapitellohumeral angle, postoperative range of motion as well as function-al outcomeduringthe Sixteen weeks and the last follow-up appointment. Results:All caseswere followed up for 1.98±1.43 years, and all fractures achieved clinical healing at 4 to 6 weeks postoperation. The surgical time and times of fluoroscopy were significantly shorter for patients in the joystick group (27.17±9.68 min, 24.25±5.92 times) when compared with CRPP group (48.59±15.75 min, 49.65±23.83 times, P<0.05). All cases showed restoration of the normal lateral capitellohumeral angle. Compared with Baumann angle of normal contralateral upper extremity during the sixteen weeks and the last follow-up appointment, the quality of reduction on the anteroposterior radiographic view was significantly better for patients in the joystick group than that of CRPP group ( P<0.05). The Baumann angle of the affected upper extremity was 77.70°±2.16°, and that of the normal contralateral upper extremity was 73.78°±4.04° in the CRPP group, joystick group was 73.06°±1.81° and 72.81°±3.45°, respectively at the sixteen weeks follow-up. The Baumann angle of the affected upper extremity was 77.13°±2.20°, and that of the normal contralateral upper extremity was 74.17°±4.17° in the CRPP group, joystick group was 72.69°±1.70° and 73.38°±3.48°, respectively at the last follow-up. The range of motion and clinical outcomes based on the criteria of Flynn were similar in both groups ( P<0.05). The elbow joint function of excellent and good rate of the criteria of Flynn was 82.61%, elbow flexion was 134.13°±8.61°, elbow extension was -3.48°±6.47° in the CRPP group, joystick group was 81.25%, 132.19°±9.48° and -3.44°± 4.37°, respectively at the sixteen weeks follow-up. The elbow joint function of excellent and good rate of the criteria of Flynn was 91.30%, elbow flexion was 140.14°±5.76°, elbow extension was -0.65±3.79° in the CRPP group, joystick group was 93.75%, 141.88°±5.12° and -0.31°±3.86°, respectivelyat the last follow-up. No immediate postoperative complications were observed. Conclusion:A transolecranon pin joystick technique combined with CRPP is a safe and effective method, can decrease surgical time and times of fluoroscopy and improve quality of reduction with no increasing risk of complications for closed reduction of multidirectionally unstable supracondylar humeral fractures in children.

3.
Chinese Journal of Biotechnology ; (12): 1715-1722, 2019.
Article in Chinese | WPRIM | ID: wpr-771759

ABSTRACT

The liver is the metabolic center of mammalian body. Systematic study on liver's proteome expression under different physiological and pathological conditions helps us understand the functional mechanisms of the liver. With the rapid development of liquid chromatography tandem mass spectrometry technique, numerous studies on liver physiology and pathology features produced a large number of proteomics data. In this paper, 834 proteomics experiments of mouse liver were systematically collected and the mouse liver proteome database (Mouse Liver Portal, http://mouseliver.com) was established. The Mouse Liver Portal contains the liver's proteomics data under different physiology and pathology conditions, such as different gender, age, circadian rhythm, cell type and different phase of partial hepatectomy, non-alcoholic fatty liver. This portal provides the changes in proteins' expression in different conditions of the liver, differently expressed proteins and the biological processes which they are involved in, potential signal transduction and regulatory networks. As the most comprehensive mouse liver proteome database, it can provide important resources and clues for liver biology research.


Subject(s)
Animals , Chromatography, Liquid , Databases, Factual , Liver , Mice , Proteome , Proteomics
4.
Article in Chinese | WPRIM | ID: wpr-743909

ABSTRACT

BACKGROUND: Steroid-induced avascular necrosis of the femoral head is a worldwide problem, and its pathogenesis still remains unclear. Its pathogenesis is still the focus of research in this field. OBJECTIVE: To discuss the role of autophagy gene Beclin1 in steroid-induced avascular necrosis of the femoral head and the effect of 3-methyladenine on Beclin1. METHODS: Thirty-six 5-month-old Japanese white rabbits (provided by Xi'an Dilepu Biological Resource Development Co., Ltd.) were included and randomly divided into control group (intramuscular injection of normal saline), avascular necrosis of the femoral head group (intramuscular injection of methylprednisolone) and 3-methyladenine group (intramuscular injection of methylprednisolone and 3-methyladenine), with 12 rabbits in each group. The injection was conducted four times, with 1 week in between. The animals from each group were sacrificed at 1, 2, 3 and 4 weeks for sample taken. Vacant bone lacunae were counted under optical microscope. mRNA and protein expression levels of Beclin1 were detected by RT-PCR and western blot assay. RESULTS AND CONCLUSION: (1) Histomorphological observation under optical microscope showed that the rate of vacant lacunae in the avascular necrosis of the femoral head and 3-methyladenine groups was significantly increased (P < 0.05), and was significantly lower in the 3-methyladenine group than that in the avascular necrosis of the femoral head group (P < 0.05). (2) Glucocorticoid could rapidly stimulate the expression of Beclin1 mRNA in the femoral head tissue, and the protein level was significantly increased at the first two weeks, suggesting that autophagy was activated. 3-Methyladenine could inhibit this process. (3) These findings indicate that enhanced autophagy gene Beclin1 expression is important for avascular necrosis of the femoral head. 3-Methyladenine can regulate Beclin1, thereby controlling autophagy, which alleviates the occurrence and development of avascular necrosis of the femoral head.

5.
Article in Chinese | WPRIM | ID: wpr-514911

ABSTRACT

BACKGROUND: Autophagy of osteocytes has been found to be implicated in the pathogenesis of steroid-induced necrosis of the femoral head and closely related to apoptosis.OBJECTIVE: To summarize the research progress of autophagy in the pathogenesis of steroid-induced necrosis of the femoral head by studying the interaction between cell autophagy and cell apoptosis as well as osteocytes.METHODS: A computer-based online retrieval of PubMed, Embase and CNKI databases was performed for relevant literatures published from October 1996 to October 2016 with the keywords of steroid, necrosis of the femoral head, cell apoptosis, cell autophagy, osteocyte in Chinese and English, respectively. The articles concerning steroid-induced necrosis of femoral head and cell autophagy were collected, and the redundant and old researches or Meta analysis were removed.RESULTS AND CONCLUSION: Mammalian target of rapamycin, Beclin-1, microtubule-associated protein 1 light chain 3, bone morphogenetic proteins, fork box protein and transcription gene family and transcription factor 4 are closely related to autophagy. The interaction between autophagy and osteocytes is correlated with steroid dose: the autophagy shows protective factor under the low dose corticosteroids; however, with the increase of the dosage, a large number of apoptotic cells, and the phenomenon of bone loss can been observed. Furthermore, the relationship of cell autophagy with apoptosis and bone mass maintenance is still controversial, which needs to be explored in depth via a series of rational experiments.

6.
Article in Chinese | WPRIM | ID: wpr-498803

ABSTRACT

It analyzed the definition, mechanism, characteristics of Mirror Visual Feedback and summarized the application of mirror visual feedback in recovering upper limb function after stroke patients at home and abroad, so as to provide evidences for the further research in China.

7.
Article in Chinese | WPRIM | ID: wpr-492673

ABSTRACT

BACKGROUND:Cilium is an antenna-like organele that plays a vital role in al organs generation and maintenance.Ciliopathiesrefer tomultisystem diseasescaused bydisordersofthecilia structure and function. OBJECTIVE:To introduce current understanding of the structure and function of the cilium,with afocus on the study progress ofciliopathies. METHODS:PubMed databasewas searched by computerusing the keywords of“primary cilium, motion cilium,ciliopathy”.Totally 371 relevantarticlespublished from 1959 to 2015 were retrieved. After excluding repetitive and irrelevant articles,we enrolled32 English literaturesregardingcilia structure, functionand ciliopathiesin accordance with the inclusion criteria. RESULTS AND CONCLUSION:Ciliopathiesalmostinvolve every system of the human body, such asthe brain, eyes, liver, kidney, bone and reproductive system.Most ciliopathies are related to human developmental or degenerative diseases, which characterized by wide-ranging clinical and genetic heterogeneity,such asprimary ciliary dyskinesia,ploycystic kidney disease, infertility, blindness and tumor. In view ofthemedical importanceof the cilium, scholars have devoted tostudyingthe underlying celular mechanisms.Thesein turnhave revealed thatciliopathiesare not only caused bydefectiveassemblyof the cilium,but also by impaired ciliarysignaling.

8.
Article in Chinese | WPRIM | ID: wpr-547149

ABSTRACT

[Objective]To explore the change and its significance of the expression of vascular endothelial growth factor(VEGF) messenger RNA and bone morphogenic protein-2(BMP-2) of the local femoral head in the nontraumatic osteonecrosis of femoral head(NONFH).[Method]Sixteen samples of femoral heads of NONFH were collected as the experimental group and fresh 10 samples of femoral heads of femoral neck fracture as control group,overall examples were collected from total articular replacement arthroplasty.The samples were splitted in coronal plane and get one bone block from necrosis area and another one from healthy area,make them into microtome section after the process of immobility and decalcification.Their pathological change was observed by using optical microscope and electron microscope and detect the expression of BMP-2 and VEGFmRNA in femoral head through making use of immunohistochemistry and in-situ hybridization technique.[Result]The organization structure of experimental group was disorganized,cracked and the bone trabecula was rarefactive and non-intact and there was a great number of empty lacuna in bone trabecula.While there was the reverse situation in the control group.The intensity and area of positive expression of BMP-2 and VEGFmRNA in femoral head of the experimental group were lower than that of control group obviously.The result showed statistical significance(P

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