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1.
Chinese Journal of Rehabilitation Theory and Practice ; (12): 1316-1326, 2023.
Article in Chinese | WPRIM | ID: wpr-998973

ABSTRACT

ObjectiveTo develop a rehabilitation program of nature posture treatment (NPT) suspension therapy based on the International Classification of Functioning, Disability and Health-Children and Youth version (ICF-CY) framework, and apply it to neurodevelopmental disorders. MethodsThe ICF-CY theoretical group (group A) and NPT suspension therapy group (group B) were established. Group A searched literature from common databases, to extract high-frequency words related to suspension therapy and match with categories of ICF-CY, to develop ICF-CY theoretical framework of the NPT suspension therapy. Group B developed specific rehabilitation procedures and training items based on the framework to compose the training pool. A total of 110 children aged less than six years with neurodevelopmental disorders and associated motor impairments were selected from outpatient or inpatient of the First Affiliated Hospital of Xinxiang Medical University, between October, 2019 and October, 2022. They were randomly divided into control group (n = 55) and clinical group (n = 55), who received routine neurodevelopmental therapy and NPT suspension therapy program based on ICF-CY, respectively, for a week. The incidence of satisfaction, acceptance and adverse events were observed. ResultsTwo cases in the control group and four cases in the clinical group dropped down. For the clinical group, the incidence of satisfaction was 98% (50/51), with acceptance of 96% (49/51), and one adverse event occurred. For the control group, the incidence of both the satisfaction and acceptance was 100%, and no adverse event occurred. There was no significant difference in the incidence of satisfaction, acceptance and the adverse event (P > 0.05). ConclusionThe NPT suspension therapy program based on the ICF-CY framework is safe and acceptable for children with neurodevelopmental disorders.

2.
Chinese Journal of Contemporary Pediatrics ; (12): 697-704, 2023.
Article in Chinese | WPRIM | ID: wpr-982015

ABSTRACT

OBJECTIVES@#To investigate the risk factors for neonatal asphyxia in Hubei Enshi Tujia and Miao Autonomous Prefecture and establish a nomogram model for predicting the risk of neonatal asphyxia.@*METHODS@#A retrospective study was conducted with 613 cases of neonatal asphyxia treated in 20 cooperative hospitals in Enshi Tujia and Miao Autonomous Prefecture from January to December 2019 as the asphyxia group, and 988 randomly selected non-asphyxia neonates born and admitted to the neonatology department of these hospitals during the same period as the control group. Univariate and multivariate analyses were used to identify risk factors for neonatal asphyxia. R software (4.2.2) was used to establish a nomogram model. Receiver operator characteristic curve, calibration curve, and decision curve analysis were used to assess the discrimination, calibration, and clinical usefulness of the model for predicting the risk of neonatal asphyxia, respectively.@*RESULTS@#Multivariate logistic regression analysis showed that minority (Tujia), male sex, premature birth, congenital malformations, abnormal fetal position, intrauterine distress, maternal occupation as a farmer, education level below high school, fewer than 9 prenatal check-ups, threatened abortion, abnormal umbilical cord, abnormal amniotic fluid, placenta previa, abruptio placentae, emergency caesarean section, and assisted delivery were independent risk factors for neonatal asphyxia (P<0.05). The area under the curve of the model for predicting the risk of neonatal asphyxia based on these risk factors was 0.748 (95%CI: 0.723-0.772). The calibration curve indicated high accuracy of the model for predicting the risk of neonatal asphyxia. The decision curve analysis showed that the model could provide a higher net benefit for neonates at risk of asphyxia.@*CONCLUSIONS@#The risk factors for neonatal asphyxia in Hubei Enshi Tujia and Miao Autonomous Prefecture are multifactorial, and the nomogram model based on these factors has good value in predicting the risk of neonatal asphyxia, which can help clinicians identify neonates at high risk of asphyxia early, and reduce the incidence of neonatal asphyxia.


Subject(s)
Infant, Newborn , Humans , Male , Pregnancy , Female , Nomograms , Retrospective Studies , Cesarean Section , Risk Factors , Asphyxia Neonatorum/etiology
3.
Chinese Journal of Rehabilitation Theory and Practice ; (12): 558-564, 2023.
Article in Chinese | WPRIM | ID: wpr-975140

ABSTRACT

ObjectiveTo systematically evaluate the effect of action observation therapy (AOT) on upper limb function in children with cerebral palsy. MethodsRelevant literatures about the effect of AOT on upper limb function in children with cerebral palsy were retrieved from the databases of PubMed, Embase, Cochrane Library, Web of Science and CNKI, from the establishment to July 9, 2022. ResultsEleven articles involving 497 patients were included, which were mainly published in the past ten years. The studies included hospital-based studies with therapist supervision and home-based studies without therapist supervision, mainly related to the improvement of upper limb function of AOT in children with cerebral palsy. Experimental group performed actions related to activities of daily living, while control group mainly watched video clips excluding actions, 15 to 120 minutes a time, three to five times a week, with most of the intervention periods of three to four weeks. AOT improved the upper limb function of children with cerebral palsy in terms of body structure and function, and activity and participation, specifically grip strength, muscle tension, and hand dexterity and function. ConclusionHospital-based AOT with therapist supervision can improve upper limb function in children with cerebral palsy, while the effect of home-based AOT without therapist supervision and the long-term effect of AOT need to be further studied.

4.
Annals of the Academy of Medicine, Singapore ; : 263-271, 2022.
Article in English | WPRIM | ID: wpr-927486

ABSTRACT

INTRODUCTION@#Infant gastroesophageal reflux disease (GERD) is a significant cause of concern to parents. This study seeks to describe GERD prevalence in infants, evaluate possible risk factors and assess common beliefs influencing management of GERD among Asian parents.@*METHODS@#Mother-infant dyads in the Singapore PREconception Study of long-Term maternal and child Outcomes (S-PRESTO) cohort were prospectively followed from preconception to 12 months post-delivery. GERD diagnosis was ascertained through the revised Infant Gastroesophageal Reflux Questionnaire (I-GERQ-R) administered at 4 time points during infancy. Data on parental perceptions and lifestyle modifications were also collected.@*RESULTS@#The prevalence of infant GERD peaked at 26.5% at age 6 weeks, decreasing to 1.1% by 12 months. Infants exclusively breastfed at 3 weeks of life had reduced odds of GERD by 1 year (adjusted odds ratio 0.43, 95% confidence interval 0.19-0.97, P=0.04). Elimination of "cold or heaty food" and "gas producing" vegetables, massaging the infant's abdomen and application of medicated oil to the infant's abdomen were quoted as major lifestyle modifications in response to GERD symptoms.@*CONCLUSION@#Prevalence of GERD in infants is highest in the first 3 months of life, and the majority outgrow it by 1 year of age. Infants exclusively breastfed at 3 weeks had reduced odds of GERD. Cultural-based changes such as elimination of "heaty or cold" food influence parental perceptions in GERD, which are unique to the Asian population. Understanding the cultural basis for parental perceptions and health-seeking behaviours is crucial in tailoring patient education appropriately for optimal management of infant GERD.


Subject(s)
Female , Humans , Infant , Infant, Newborn , Male , Gastroesophageal Reflux/epidemiology , Parents/psychology , Prevalence , Risk Factors , Singapore/epidemiology
5.
Chinese Journal of Rehabilitation Theory and Practice ; (12): 183-189, 2022.
Article in Chinese | WPRIM | ID: wpr-923513

ABSTRACT

@#Objective To systematically review the research progress of sensory integration therapy in the rehabilitation of children with cerebral palsy. Methods The literatures related to the application of sensory integration therapy in the rehabilitation of children with cerebral palsy was retrieved from Web of Sciences, PubMed, CNKI, Wanfang data, and VIP databases until November 27, 2021, using subject search. And the contents of the literatures were extracted to review the implementation plan of sensory integration therapy and the effectiveness of its application in the rehabilitation of children with cerebral palsy. Results Six literatures were included. The main focus was on the research of sensory integration therapy on postural control, gross motor function, intelligence level and cognitive function, and treatment modalities in children with cerebral palsy. Conclusion Sensory integration therapy is effective on motor function, posture control and intelligence in children with cerebral palsy. To maximize the effect of sensory integration therapy, a suitable rehabilitation treatment plan should be formulated according to the degree of sensory integration disorder and the age, gender and tolerance of children.

6.
Chinese Journal of Applied Clinical Pediatrics ; (24): 502-509, 2022.
Article in Chinese | WPRIM | ID: wpr-930465

ABSTRACT

This review aims to interpret the interventions to improve physical function for children and young people with cerebral palsy, thus providing relevant suggestions.Relevant literatures published before November 2018 were systematically searched in Cochrane library, CINAHL, and Embase MEDLINE using the Grading of Recommendations Assessment, Development and Evaluation method.Consult with international experts and patients to assess evidence and recommend it.Based on 3 systematic reviews, 30 randomized clinical trials, and 5 pre-and post-treatment studies, a total of 13 recommendations were given.The guidelines recommend that, in order to achieve functional stan-dards, intervention measures should include, patient-selected goals, full-task practice in real-life settings, support for family empowerment, and a team approach.The age, ability, and child/family preferences were all needed to be considered.In order to improve walking ability, ground walking and treadmill training can be carried out.Various methods can promote the realization of hand use goals hand use, including the two-hand exercise training, constraint-induced moverment therapy, goal-oriented training and cognitive therapy.In terms of patient self-care, the guideline proposed that, the combination of full-task practice and auxiliary equipment can improve the independence of self-care and reduce the burden of care givers.Leisure goals could be achieved by the combination of the practice of the entire task with strategies to address environmental, personal, and social barriers.The intervention of children and adolescents with cerebral palsy should take into consideration of patient selection and the goal of full-task practice.The child/family preference, age and ability should be considered when clinical workers selecting specific interventions.

7.
Chinese Journal of Rehabilitation Theory and Practice ; (12): 97-101, 2021.
Article in Chinese | WPRIM | ID: wpr-905318

ABSTRACT

The nervous system controls eccentric exercise in a special way. During eccentric exercise, excitability increases in cortex, and inhibition decreases, causing excitability decrease in spinal cords. In another hand, increased cortical excitability results in extra excitatory compensation for spinal inhibition. The excitability of the corticospinal pathway depends on the balance between excitability and inhibition of the spinal cord level finally, which usually decreases. Many factors, such as the intensity of contraction, can affect the balance of corticospinal excitability. There is a cross-over effect in eccentric exercise, which promotes corticospinal excitability in untrained limbs. However, the effects and mechanisms of muscle length, fatigue and training duration are still unclear, and current researches have focused in the healthy populations. More researches are needed to explore the effects of eccentric exercise on ill populations.

8.
Chinese Journal of Rehabilitation Theory and Practice ; (12): 869-880, 2020.
Article in Chinese | WPRIM | ID: wpr-905405

ABSTRACT

This article summarized an overview of the 40 years development of rehabilitation for children in China and its major progress and achievements in an objective and comprehensive manner, mainly around the following four aspects: historic evolution, academic activities and achievements, international communication and cooperation, as well as challenges and prospects. It also puts forward the challenges and problems to be solved in the rehabilitation for children in China.

9.
Chinese Journal of Contemporary Pediatrics ; (12): 942-947, 2020.
Article in Chinese | WPRIM | ID: wpr-828641

ABSTRACT

OBJECTIVE@#To investigate the clinical features and outcome of neonatal acute respiratory distress syndrome (ARDS) in southwest Hubei, China.@*METHODS@#According to the Montreux definition of neonatal ARDS, a retrospective clinical epidemiological investigation was performed on the medical data of neonates with ARDS who were admitted to Department of Neonatology/Pediatrics in 17 level 2 or level 3 hospitals in southwest Hubei from January to December, 2017.@*RESULTS@#A total of 7 150 neonates were admitted to the 17 hospitals in southwest Hubei during 2017 and 66 (0.92%) were diagnosed with ARDS. Among the 66 neonates with ARDS, 23 (35%) had mild ARDS, 28 (42%) had moderate ARDS, and 15 (23%) had severe ARDS. The main primary diseases for neonatal ARDS were perinatal asphyxia in 23 neonates (35%), pneumonia in 18 neonates (27%), sepsis in 12 neonates (18%), and meconium aspiration syndrome in 10 neonates (15%). Among the 66 neonates with ARDS, 10 neonates (15%) were born to the mothers with an age of ≥35 years, 30 neonates (45%) suffered from intrauterine distress, 32 neonates (49%) had a 1-minute Apgar score of 0 to 7 points, 24 neonates (36%) had abnormal fetal heart monitoring results, and 21 neonates (32%) experienced meconium staining of amniotic fluid. Intraventricular hemorrhage was the most common comorbidity (12 neonates), followed by neonatal shock (9 neonates) and patent ductus arteriosus (8 neonates). All 66 neonates with ARDS were treated with mechanical ventilation in addition to the treatment for primary diseases. Among the 66 neonates with ARDS, 10 died, with a mortality rate of 15% (10/66), and 56 neonates were improved or cured, with a survival rate of 85% (56/66).@*CONCLUSIONS@#Neonatal ARDS in southwest Hubei is mostly mild or moderate. Perinatal asphyxia and infection may be the main causes of neonatal ARDS in this area. Intraventricular hemorrhage is the most common comorbidity. Neonates with ARDS tend to have a high survival rate after multimodality treatment.


Subject(s)
Female , Humans , Infant, Newborn , Pregnancy , China , Meconium Aspiration Syndrome , Respiratory Distress Syndrome, Newborn , Retrospective Studies
10.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 178-185, 2020.
Article in Chinese | WPRIM | ID: wpr-862676

ABSTRACT

Objective::Based on gene array technology, gene set enrichment analysis (GSEA) and immune infiltration analysis were performed on chip data of intracranial aneurysm (IA) mRNA expression profile, in order to provide theoretical basis for understanding the formation mechanism of IA. Method::The GSE75436 raw data were obtained from the gene expression omnibus (GEO). GSEA of biological process (BP) in gene ontology (GO) and Kyoto gene and genome encyclopedia (KEGG) signaling pathways were analyzed for gene expression profile by R software. The CIBERSORT deconvolution method was used to analyze the infiltration ratio of 22 types of immune cells in the expression profile. And COREMINE database was used to predict traditional Chinese medicines (TCMs), which were significant correlation with the enrichment result. Result::The GSEA results showed that the changes in gene expression of IA samples mainly involved in the regulation of cytokines, activation and differentiation of leukocyte, inflammatory immune response and other processes. The infiltration matrix analysis of immune cells showed that mast cells resting and neutrophils were significantly reduced in IA samples. The comparison of paired samples showed that mast cells and natural killer cells (NK cells) were significantly activated in the IA samples of the same individual, while neutrophils and T cells CD4 naive were significantly reduced. Through COREMINE prediction, it was found that Stephaniae Tetrandrae Radix was correlated with the activation of granulocytes, Sapindi Mukorossi Semen and Pistaciae Chinensis Cortex were correlated with the activation of neutrophils, Trichosanthis Semen, Paeoniae Radix Alba and Ligustri Lucidi Fructus were correlated with the cytotoxicity mediated by NK cells. Conclusion::Activation of mast cells and NK cells are closely associated with the occurrence and development of IA. The inflammatory immune processes and pathways such as nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) signaling pathway and cytotoxicity mediated by NK cells may be important factors in the pathogenesis of IA, and TCMs such as Stephaniae Tetrandrae Radix may be the potential molecular drug sources.

11.
Chinese Journal of Applied Clinical Pediatrics ; (24): 1237-1240, 2019.
Article in Chinese | WPRIM | ID: wpr-802791

ABSTRACT

Objective@#To evaluate the therapeutic effect of mouse nerve growth factor (mNGF) combined with conventional rehabilitation training on children with cerebral palsy (CP).@*Methods@#According to the inclusion criteria, 60 children with CP(spastic diplegia) who were treated at the Third Affiliated Hospital of Jiamusi University were selected by double-blind method from June to December in 2016.Sixty children with CP were stratified according to age, gender and Gross Motor Function Classification System (GMFCS) (the subjects were classified into 2 age groups: 0-2 years old and 2-4 years old), and then they were randomly divided by draw method: the control group received routine rehabilitation; the experimental group received mNGF additionally.The children with CP in the experimental group were treated with 18 μg mNGF every day for 10 days and then discontinued for 5 days, with 20 injections per month for 1 course, a total of 3 courses.After 3 courses of treatment, the changes in Gesell Development Diagnosis Schedules (GDDS), Peabody Developmental Motor Scales-Gross Motor (PDMS-GM), Peabody Developmental Motor Scales-Fine Motor (PDMS-FM) and Range of Motion (ROM) of the lower extremity were compared between the 2 groups.@*Results@#After 3 months of treatment, the changes of Gesell(gross motor: 57.40±18.13, 44.87±10.95; fine motor: 64.83±18.04, 62.60±17.34; adaptability: 76.07±14.99, 70.57±11.19; language: 74.20±15.07, 71.23±13.38, personal-social interaction: 67.40±14.10, 61.40±12.96), PDMS-GM(94.33±16.03, 81.13±20.15), PDMS-FM scores(91.53±19.73, 91.10±15.84) and ROM[adductors angle: left (69.67±22.2)°, (49.17±21.82)°; right (69.83±21.63)°, (49.67±21.61)°; popliteal angle: left (160.08±30.02)°, (125.50±25.78)°; right (160.17±22.46)°, (126.00±25.31)°; dorsal flexion angle of foot: left (10.17±6.09)°, (4.33±7.28)°; right (9.83±6.23)°, (4.83±7.48)°] in the experimental group and the control group were all significantly higher than those before treatment, and the differences were statistically significant (all P<0.05). After 3 months of treatment, the Gesell gross motor(57.40±18.13) and PDSM-GM scores(94.33±16.03) in the experimental group were significantly higher than those (44.87±10.95, 81.13±20.15, respectively) in the control group, and the differences were statistically significant (all P<0.05).@*Conclusions@#Conventional rehabilitation therapy combined with mNGF has more significant effect on the development and motor function of children with CP than routine rehabilitation training.

12.
Chinese Journal of Rehabilitation Theory and Practice ; (12): 173-178, 2018.
Article in Chinese | WPRIM | ID: wpr-702463

ABSTRACT

This article introduced the characteristics of eccentric contraction and its mechanism,as well as its application in the re-habilitation of chronic heart disease,chronic obstructive pulmonary disease,sports injuries,elderly sarcopenia,anterior cruciate ligament repair,cancer survivor,type 2 diabetes and nervous system diseases,etc.

13.
Chinese Journal of Applied Physiology ; (6): 111-114, 2018.
Article in Chinese | WPRIM | ID: wpr-773792

ABSTRACT

OBJECTIVES@#To identify the genotype of (APP/PS1) transgenic mice and evaluate the changing of cognitive and behavioral fu nctions, provide an effective animal model for the Alzheimer's disease (AD) research.@*METHODS@#Male APP/PS1 transgenic mice mated with female APP/PS1 transgenic mice, and the genotype of their filial mice was identified by PCR. The APP +/PS1 + mice were assigned into AD model group (AD group, =8), and the APP/PS1 mice were assigned into control group (CT group, =8). The Morris water maze test was carried out to detect the capacity of learning and memory of mice. After that, the mice were sacrificed and the brain tissues were sampled and stained by HE and congo red for the pathological examination.@*RESULTS@#①A APP/PS1 genome DNA about 360 bp size was detected. The methods of feeding and breeding were successful to attain APP/PS1 transgenic mice.②Statistical significance was found in the differences of the capacity of learning and memory between 7-month-old APP/PS1 positive mice and negative mice (<0.05).③The results of HE stain showed that the structure and cellular morphology of hippocampus of AD mice were obviously abnormal. The results of congo red stain showed that positive amyloid plaque was observed in brains of AD mice.@*CONCLUSIONS@#APP/PS1 transgenic mice present typical symptoms and behaviors of Alzheimer's disease. The transgenic mouse is an effective tool for the research and prevention of AD.


Subject(s)
Animals , Female , Male , Mice , Alzheimer Disease , Amyloid beta-Protein Precursor , Genetics , Disease Models, Animal , Genotype , Hippocampus , Pathology , Maze Learning , Mice, Inbred C57BL , Mice, Transgenic , Plaque, Amyloid , Pathology , Presenilin-1 , Genetics
14.
Chinese Journal of Practical Nursing ; (36): 961-965, 2018.
Article in Chinese | WPRIM | ID: wpr-697125

ABSTRACT

Objective To explore oncology nurses′attitudes and beliefs and its influencing factors toward sexuality care. Methods A total of 208 oncology nurses were investigated by convenience sampling method. The nurses were investigated with the Sexuality Attitudes and Beliefs Survey Scale and the Literacy of Sexual Health Care Scale. Results The total score of attitudes and beliefs of oncology nurses toward sexuality care was (44.88 ± 11.45). Multivariate linear regression analysis showed that age, sexual health education and training experience, knowledge of sexual health care and efficacy of sexual health care were the influencing factors of SABS. A total of 41.2%variance was explained. Conclusions We should strengthen the training of oncology nurses about sexuality care to reduce the barriers of sexual health education in clinical work and help nursesform the right attitudes and beliefs of health care so that they can provide patients with better care.

15.
Chinese Journal of Applied Clinical Pediatrics ; (24): 378-383, 2018.
Article in Chinese | WPRIM | ID: wpr-696400

ABSTRACT

Objective To investigate the epidemiological characteristics of cerebral palsy(CP)in children aged 1-6 years in China,including the incidence,prevalence,type of CP,etiology,prevention and rehabilitation status. Methods The survey was carried out by standard questionnaires,multi-center collaboration,stratified-cluster ran-dom sampling method.The surveyed adopted the following principles:streets in the city and villages in the rural areas, and the number of the urban and rural children was the same,and the proportion of children in each age group was balanced.The investigation areas included provinces and autonomous regions,including Heilongjiang,Beijing,Henan, Shandong,Shanxi,Shaanxi,Anhui,Hunan,Guangxi,Guangdong,Chongqing and Qinghai,and 323 858 children were in-vestigated.Results The incidence of CP was 2.48‰(155/62 591 cases),and the prevalence was 2.46‰(797/323 858 cases)(1-6 years old).The prevalence varied in different regions,in which the highest prevalence was 5. 40‰(54/9 998 cases)in Qinghai province,and the lowest prevalence was 1.04‰(47/45 133 cases)in Shandong province.The prevalence of the males(2.64‰,461/174 391 cases)was higher than that of the females(2.25‰, 336/149 467 cases),and the difference was statistically significant(P<0.05).The types of CP were spastic type (58.85%,469/797 cases),mixed type(13.17%,105/797 cases),dyskinetic(9.79%,78/797 cases),hypotonic (8.28%,66/797 cases),ataxia(6.25%,52/797 cases)and rigid(3.39%,27/797 cases)respectively in 797 CP children.The first three risk factors for CP were long -term exposure to harmful physical factors during pregnancy, whether there were birth defects among the three generations of relatives of the children,such as children's peers, parents or grandparents,whether there were birth defects among the children's peers,parents or grandparents,and neonatal jaundice or persistent jaundice.Among 797 CP children,79.67% of the children with CP were timely detected and treated in the local hospitals,while the other 19.93% of them were not timely treated.The places which could give them timely detection and early diagnosis and treatment were general hospitals(42.97%),Maternity and Infant Hospitals (27.03%)and Children's Hospitals(20.31%). The main rehabilitation methods for 797 children with CP were 34.58% in the hospitals or rehabilitation centers,31.61% in the communities(including at home),33.80% mainly in the medical institution,and in the communities they could also receive partially rehabilitation services. Conclusions The prevalence of CP in China is coincident with international levels.The prevalence rate of CP in males is higher than that in females.The types of CP distribution are accorded with international distribution characteristics.There were still some children with CP who could not receive timely detection and treatment.Rehabilitation at the medical institutions is the chief way and proper rehabilitation guidance should be carried out in the communities.

16.
Journal of Medical Postgraduates ; (12): 725-729, 2017.
Article in Chinese | WPRIM | ID: wpr-617607

ABSTRACT

Objective Puerarin can remove inflammatory mediators, but the underlying mechanisms are not yet clear.The purpose of this study is to observe the synthesis of IL-6 in co-cultured bronchial epithelial (Beas-2B) and neutrophil cells, and to investigate the inhibitory effect of puerarin on IL-6.Methods Beas-2B and neutrophil cells were extracted and divided into six groups: neutrophil cells cultured alone (NC), Beas-2B cells cultured alone (BC), Beas-2B and neutrophil cells co-cultured (BC+NC), and Beas-2B and neutrophil cells co-cultured with puerarin at 50 μg/mL (BC+NC+P50), 100 μg/mL (BC+NC+P100) and 200 μg/mL (BC+NC+P200).The concentrations of IL-6 in different groups were quantitatively measured by ELISA and the expression of the IL-6 gene detected by real-time PCR.Results After 18 hours of culturing, the concentration of IL-6 was significantly increased in the BC+NC group ([280.409±21.340] pg/mL) as compared with those in BC ([240.002±23.727] pg/mL), NC ([22.771±1.146] pg/mL), BC+NC+P50 ([244.205±9.335] pg/mL), BC+NC+P100 ([218.168±18.635] pg/mL), and BC+NC+P200 ([179.539±7.340] pg/mL) (P<0.05), but lower in the BC+NC+P200 than in the BC+NC+P50 and BC+NC+P100 groups (P<0.05).The expression of the IL-6 gene was markedly up-regulated in the BC+NC group (1.515±0.013) in comparison with those in BC (1.000±0.215), BC+NC+P50 (0.398±0.024), BC+NC+P100 (0.332±0.016), and BC+NC+P200 (0.306±0.015) (P<0.05), but lower in the BC+NC+P200 than in the BC and BC+NC+P50 groups (P<0.05).Conclusion Direct-contact co-culture of Beas-2B and neutrophil cells significantly enhances the synthesis and release of the inflammatory mediator IL-6, which can be inhibited by puerarin.

17.
China Journal of Chinese Materia Medica ; (24): 3906-3911, 2017.
Article in Chinese | WPRIM | ID: wpr-335764

ABSTRACT

To establish an on-line monitoring method for extraction process of Schisandrae Chinensis Fructus, the formula medicinal material of Yiqi Fumai lyophilized injection by combining near infrared spectroscopy with multi-variable data analysis technology. The multivariate statistical process control (MSPC) model was established based on 5 normal batches in production and 2 test batches were monitored by PC scores, DModX and Hotelling T2 control charts. The results showed that MSPC model had a good monitoring ability for the extraction process. The application of the MSPC model to actual production process could effectively achieve on-line monitoring for extraction process of Schisandrae Chinensis Fructus, and can reflect the change of material properties in the production process in real time. This established process monitoring method could provide reference for the application of process analysis technology in the process quality control of traditional Chinese medicine injections.

18.
Chinese Journal of Zoonoses ; (12): 848-852, 2017.
Article in Chinese | WPRIM | ID: wpr-659516

ABSTRACT

We investigated the characteristics and epidemiological characteristics of severe fever with thrombocytopenia syndrome (SFTS) infection in Tiantai County,Zhejiang Province.Active surveillance and epidemiological investigation were employed with the serum samples collected simultaneously.The samples were detected by fluorescence quantitative PCR assay recommended by national standard of China.By descriptive epidemiology,the SFTS cases in 2012-2015 in Tiantai and related data were analyzed.In 2012-2015,there were 12 new cases of SFTS infection and 2 deaths,including 5 males and 7 females (39 to 75 yrs).The peak incidence was found from April to August.The main clinical manifestations was fever (100 %),chills and fatigue (83%),and body pain (75%).The positive rate of nucleic acid screened in subclinical population was 1.4%;8 ca ses with definite records of tick bites (67 %).In conclusion,several cases of SFTS have been confirmed in Tiantai County with two inapparent infections,which suggest the urgency and importance to strengthen the active surveillance,prevention and control measures and health education s in the coming days.

19.
Chinese Journal of Zoonoses ; (12): 848-852, 2017.
Article in Chinese | WPRIM | ID: wpr-657441

ABSTRACT

We investigated the characteristics and epidemiological characteristics of severe fever with thrombocytopenia syndrome (SFTS) infection in Tiantai County,Zhejiang Province.Active surveillance and epidemiological investigation were employed with the serum samples collected simultaneously.The samples were detected by fluorescence quantitative PCR assay recommended by national standard of China.By descriptive epidemiology,the SFTS cases in 2012-2015 in Tiantai and related data were analyzed.In 2012-2015,there were 12 new cases of SFTS infection and 2 deaths,including 5 males and 7 females (39 to 75 yrs).The peak incidence was found from April to August.The main clinical manifestations was fever (100 %),chills and fatigue (83%),and body pain (75%).The positive rate of nucleic acid screened in subclinical population was 1.4%;8 ca ses with definite records of tick bites (67 %).In conclusion,several cases of SFTS have been confirmed in Tiantai County with two inapparent infections,which suggest the urgency and importance to strengthen the active surveillance,prevention and control measures and health education s in the coming days.

20.
Basic & Clinical Medicine ; (12): 138-141, 2017.
Article in Chinese | WPRIM | ID: wpr-508993

ABSTRACT

Objective Evaluating the effect of situational questions for qualifying the students'abilities in physiolo-gy examination .Methods Comparing the difficulty coefficients and discrimination indexes between situational and traditional choice questions .Results Compared with the traditional choice questions , the difficulty coefficient of situational questions increased , while the discrimination indexes were more reasonable .The discrimination indexes of situational understanding questions were higher than those of the traditional memory and understanding questions . There were no difference between discrimination indexes of the situational application questions and those of tradi -tional application questions .Conclusions Situational questions not only improved the quality of examinations , but also facilitate evaluating students'learning ability .

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