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1.
International Eye Science ; (12): 1608-1614, 2022.
Article in Chinese | WPRIM | ID: wpr-942826

ABSTRACT

AIM: To investigate the effect of lncRNA MALAT1 on the proliferation, migration and angiogenesis of retinal vascular endothelial cells and its molecular mechanism.METHODS: The expression levels of lncRNA MALAT1 in plasma of normal control group, diabetic without retinopathy group and diabetic retinopathy group were detected by qPCR and the effect of glucose culture on the expression levels of lncRNA MALAT1 were detected by qPCR too. The expression level of miR-124-3p was detected by qRT-PCR; Western blotting was used to detect the expression level of SOX7; The targeting relationship between lncRNA MALAT1 and miR-124-3p, miR-124-3p and SOX7 were detected by the dual-luciferase reporter system; CCK-8 assay was used to detect cell proliferation activity; Transwell assay was used to detect the migration ability of cells; Angiogenesis of hRMECs cells was measured by in vitro tube formation assay.RESULTS:The expression level of lncRNA MALAT1 in plasma of diabetic retinopathy patients was significantly higher than that of diabetic without retinopathy group and normal control group(P<0.001). In vitro glucose culture significantly promoted the expression of lncRNA MALAT1 in hRMECs cells, as well as the proliferation, migration and angiogenesis of hRMECs cells(all P<0.05). Knockdown of lncRNA MALAT1 significantly inhibited the proliferation, migration and tubule formation of hRMECs cells(all P<0.05). Dual-luciferase reporter gene assay showed that lncRNA MALAT1 targeted with miR-124-3p, and miR-124-3p targeted with SOX7. Overexpression of miR-124-3p significantly inhibited the proliferation, migration and tubule formation of hRMECs cells(all P<0.05). Overexpression of lncRNA MALAT1+miR-124-3p, miR-124-3p+SOX7, and knockdown of lncRNA MALAT1+overexpression of SOX7 all significantly eliminated the inhibitory effect of hRMECs cells(all P<0.05).CONCLUSION: lncRNA MALAT1 promote the proliferation, migration and angiogenesis of retinal endothelial cells in diabetic retinopathy by down-regulating the negative regulation of miR-124-3p on SOX7. Therefore, abnormal upregulation of lncRNA MALAT1 in patients with diabetic retinopathy is a potential biomarker.

2.
Article in Chinese | WPRIM | ID: wpr-942371

ABSTRACT

Biological category is effective to indicate the evolution of organism populations between past and present. Conventional taxonomy of human parasites mainly depends on important morphological features, which suffers from a problem of categorizing related-genera species with similar morphological characteristics. With recent advances in molecular biological technologies, the effective applications of mitochondrial and ribosomal biomarkers and sequencing greatly improve the development of the taxonomic rank of human parasites. Worldwide, the classification of human parasites have been continuously revised and improved. Hereby, we re-categorize parasitic Protozoa, Trematoda, Cestoda and Nematoda, so as to provide insights into the researches on molecular systematics and genetic evolution of human parasites.

3.
Article in Chinese | WPRIM | ID: wpr-940816

ABSTRACT

Yunüjian (Jing Yue's Collected Works), composed of Gypsum Fibrosum, Rehmanniae Radix Praeparata, Anemarrhenae Rhizoma, Ophiopogonis Radix, and Achyranthis Bidentatae Radix, is used for the treatment of "headache, toothache, consumptive thirst, and other diseases caused by heat and Yin deficiency in the stomach". In modern clinical settings, it has definite hypoglycemic efficacy, as it improves insulin resistance, inhibits oxidative stress and inflammatory response, and down-regulates estrogen level. It is often directly used, modified before use, combined with other formulae (Liuwei Dihuangtang, Shashen Maidongtang, Shenqi Sanjingtang, Huangqi Guizhi Wuwutang, Zengyetang, Zengye Baihutang, etc.), or used together with western medicine (insulin, metformin, pioglitazone, glurenorm, etc.). Modified Yunüjian can be applied for the treatment of type 2 diabetes mellitus, gestational diabetes, polycystic ovary syndrome with insulin resistance, pruritus induced by diabetes mellitus, diabetic peripheral neuropathy, diabetic nephropathy, diabetic ketoacidosis, diabetic retinopathy, and diabetic periodontitis. According to previous experiment, the protection for pancreatic islet β-cells is an important way of Yunüjian to improve diabetes mellitus. The formula can remove damaged proteins and organelles by increasing the expression of autophagy-related 2B (Atg2B), ubiquitin-like-conjugating enzyme (Atg3), γ-aminobutyric acid type A receptor-associated protein (GABARAP), selective autophagy receptor (p62/SQSTM1), etc. Moreover, it plays an active role in islet cell proliferation and apoptosis by regulating the expression of mammalian target of rapamycin (mTOR) and reducing the expression of islet β-cell autophagy gene (Beclin). These are pivotal for maintaining the quantitative structure and functions of islet cells. The modulatory effect of Yunüjian on growth hormone-releasing peptide (ghrelin), gastrin, and growth hormone secretagogue receptor (GHSR) mRNA expression is also the underlying mechanism for the formula to protect pancreatic β-cells. In this study, we summarized the clinical and experimental studies on the therapeutic effect of this formula on diabetes mellitus, discussed the mechanisms, and proposed suggestions on the problems, hoping to provide a reference for the clinical application and further development of Yunüjian. This study is of practical significance for the scientific interpretation of the modern connotation of Yunüjian and the expansion of its clinical application.

4.
Article in Chinese | WPRIM | ID: wpr-940689

ABSTRACT

ObjectiveTo observe the effects of Sinisan on behaviors and NOD-like receptor protein 3 (NLRP3) inflammasomes of depressed rats induced by chronic unpredictable mild stress (CUMS) and further explore the anti-depressant mechanism of Sinisan. MethodFifty male rats were randomly divided into a normal group, a model group, an NLRP3 inhibitor (MCC950) group (10 mg·kg-1), and low- (2.5 g·kg-1) and high-dose (5 g·kg-1) Sinisan groups, with 10 rats in each group. The depression model was induced by 42 d CUMS in rats except for those in the normal group. Drug intervention was performed on the 22nd day of modeling by gavage in the Sinisan groups and by intraperitoneal injection in the MCC950 group. Except for the MCC950 group, the remaining four groups received 10 mg·kg-1 physiological saline by intraperitoneal injection, while the rats in the model group, the normal group, and the MCC950 group were administered with 3 mL of physiological saline by gavage. Twenty-one days later, the sucrose preference test and open field test were performed. Western blot was used to detect the protein expression of NLRP3, apoptosis-associated speck-like protein containing a CARD (ASC), cysteinyl aspartate-specific protease-1 (Caspase-1), B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), ionized calcium-binding adapter molecule 1 (Iba1), and CD68 in the hippocampus of rats in each group. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of interleukin-1β (IL-1β) and interleukin-18 (IL-18) in the hippocampus of rats. Nissl staining and TUNEL were used to assess the pathological changes and apoptosis level in the hippocampal CA1 region of rats, respectively. ResultThe sucrose preference rate and consumption volume in the sucrose preference test, the total distance, the percentage of central movement distance, and the percentage of residence time in the open field test of rats in the model group were lower than those in the normal group (P<0.01). Compared with the model group, the Sinisan groups and the MCC950 group showed improved depression-like behaviors, apoptosis level in the hippocampal CA1 region, and neuron loss to varying degrees. Sinisan could reduce the levels of IL-1β, IL-18, Bax, Iba1, and CD68 in the hippocampus (P<0.05, P<0.01), increase the level of Bcl-2 (P<0.05, P<0.01), and inhibit the protein expression of NLRP3, ASC, and Caspase-1 related to NLRP3 inflammasomes (P<0.05, P<0.01). ConclusionSinisan can improve the depression-like behaviors and pathological damage of hippocampal neurons in CUMS-induced rats, and the mechanism may be related to the inflammatory response mediated by the NLRP3 inflammasome signaling pathway.

5.
Article in Chinese | WPRIM | ID: wpr-940643

ABSTRACT

Gouty arthritis (GA) is the metabolic rheumatism caused by purine metabolism disorder, which can be acute or chronic. The main manifestations of GA include recurrent redness, swelling, heat pain, and dysfunction of the affected joints. According to the theory of modern medicine, GA is closely associated with the increase in uric acid, the participation of inflammatory cytokines, the weakening of antioxidant response, apoptosis, and the imbalance of intestinal flora and bone metabolism, whereas the specific pathogenesis remains unclear. GA is characterized by easy diagnosis, difficult treatment, and high recurrence rate, which seriously affects the life quality of patients. Colchicine, corticosteroids, non-steroidal anti-inflammatory drugs, and selective cyclooxygenase-2 inhibitors are the commonly used western medicines for this disease, which demonstrate remarkable short-term therapeutic effect. However, long-term use of these medicines will bring serious adverse reactions. Chinese medicines, with high safety and causing few adverse reactions, have a variety of active components which can act on multiple pathways and targets to exert synergistic effects, thus attracting wide attention. This paper systematically reviews the literature reporting the Chinese medicines in improving antioxidant response, reducing chondrocyte apoptosis, and regulating intestinal flora and bone metabolism, aiming to further clarify the pathogenesis of GA and provide a scientific basis for the clinical application of Chinese medicines in the prevention and treatment of GA.

6.
Article in Chinese | WPRIM | ID: wpr-940483

ABSTRACT

ObjectiveTo observe the intervention of modified Sanrentang on the lipopolysaccharide-induced proliferation of rat glomerular mesangial cells, the phosphatidylinositol-3 kinase(PI3K)/protein kinase B(PKB/Akt)/nuclear factor kappa B(NF-κB) signaling pathway, and to investigate its mechanism in improving kidney inflammation in rats with immunoglobulin A nephropathy(IgAN). MethodThe 18 rats were divided into 3 groups by serum pharmacology method: normal group, high-dose and low-dose (20.70,10.35 g·kg-1·d-1) groups with 6 rats in each group. Modified Sanrentang high- and low-dose groups were intragastric with the corresponding solution of modified Sanrentang, and normal group was intragastric with equal volume of distilled water. After 5 days of intragastric administration, blood samples were collected to prepare drug-containing serum. Rat mesangial (HBZY-1) were divided into five groups of normal group, LPS 10 mg·L-1 in the model group, benazepril(50 μmol·L-1), modified Sanrentang high- and low-dose group. Preclude the use of methyl thiazolyl tetrazolium(MTT) method detect the proliferation activity of HBZY-1 cells, enzyme-linked immunosorbent assay(ELISA) was used to determine the content of each group type Ⅳ collagen(ColⅣ),Western blot and Real-time fluorescence quantitative polymerase chain reaction(Real-time PCR) were used to detect protein and mRNA expression levels of PI3K/Akt/NF-κB signaling pathway. ResultAs compared with the normal group, MTT assay showed that exposure to LPS significantly enhanced the proliferative activity, the ColⅣ was increased significantly of HBZY-1 cells(P<0.01), p-Akt, p-p65 was increased significantly (P<0.01). Compared with the model group, the proliferation and ColⅣ of rat chronic glomerulonephritis cells induced by LPS by inhibiting PI3K/Akt/NF-κB signaling pathway(P<0.01), and the phosphorylation of Akt was significantly inhibited(P<0.01), the expression levels of NF-κB p65 was reduced in modified Sanrentang high-dose group(P<0.01). ConclusionModified Sanrentang could inhibit cell proliferation and the content of ColⅣ in rat mesangial cells induced by LPS, and its mechanism might be related to suppression of PI3K/Akt signaling pathway.

7.
Article in Chinese | WPRIM | ID: wpr-940456

ABSTRACT

ObjectiveTo study the effect of Longshengzhi capsule (LSZC) on high fat diet (HFD)-induced atherosclerosis (AS) in apolipoprotein E knockout (ApoE-/-) mice. MethodApoE-/- mice were fed with HFD for 8 weeks to induce AS. Then the mice were randomized into model group, simvastatin group (4 mg·kg-1), high-dose LSZC group (1.6 g·kg-1), medium-dose LSZC group (0.8 g·kg-1), and low-dose LSZC group (0.4 g·kg-1). C57BL/6J Mice with normal diet were used as the blank control. After 10 weeks, serum levels of triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), superoxide dismutase (SOD), interleukin-1β (IL-1β), and interleukin-6 (IL-6) were detected. Hematoxylin-eosin (HE) and oil red O were used to detect aortic plaque in each group. The levels of CD34 and F4/80 in aorta were determined by immunohistochemistry (IHC). ResultCompared with the blank control, the model group demonstrated obvious aortic plaque, a large amount of lipid accumulation, serious damage of aortic intima, increase in serum levels of TC, TG, LDL-C, HDL-C, MDA, IL-1β, and IL-6 (P<0.01), decrease in SOD level (P<0.01), and rise of the expression of CD34 and F4/80 (P<0.01). Compared with the model group, LSZC of the three doses all decreased the serum levels of TG and LDL-C (P<0.05), and the levels of IL-1β and IL-6 (P<0.05, P<0.01), and the high-dose and medium-dose LSZC improved SOD level, decreased MDA content (P<0.05, P<0.01), and reduced the expression of the CD34 and F4/80 in blood vessels (P<0.05, P<0.01). ConclusionLSZC has certain intervention effect on the formation of aortic plaque in atherosclerosis ApoE-/- mice. The mechanism is that it reduces the levels of serum TG and LDL-C to lower blood lipid, decreases MDA level and improves SOD activity to inhibit lipid peroxidation, lowers the levels of IL-1β and IL-6 and down-regulates the expression of CD34 and F4/80 to protect blood vessels from inflammatory damage.

8.
Article in Chinese | WPRIM | ID: wpr-940398

ABSTRACT

ObjectiveTo compare the effect of different solvent extracts of spore powder and fruiting body of Lasiosphaera Calvatia on fibroblasts and wound healing of full-thickness skin defect, in order to screen the optimal medication part of Lasiosphaera Calvatia. MethodThe effect of water extract and alcohol extract of spore powder and fruiting body on cell proliferation and cell migration of mouse skin fibroblasts (MSF) were examined in vitro. Cell proliferation and activity test (CCK-8) method was used for cell proliferation, scratch assay was used for cell migration, flow cytometry was conducted to explore cell cycle, enzyme-linked immunosorbent assay (ELISA) was used to determine the production of collagen Ⅰ and Ⅲ. At the same time, a full-thickness skin defect wound model was established to investigate the therapeutic effect of different solvent extracts of spore powder. Ultraviolet-visible spectrophotometry was used to measure the contents of index components in different solvent extracts. ResultThe water extract of spore powder and fruiting body had certain cytotoxicity, while the alcohol extract could promote proliferation, migration and production of collagen Ⅰ and Ⅲ of MSF, and the effect of spore powder was significantly higher than that of fruiting body. When the concentration was 10 mg·L-1, the cell proliferation rate of alcohol extract of spore powder was as high as (159.22±15.95)%, and could promote MSF from the G0/G1 phase to S phase and G2/M phase with an increased proliferation index. The alcohol extract also promoted the migration of fibroblasts, secreted collagen Ⅰ and Ⅲ. On in vivo model, the alcohol extract of spore powder significantly accelerated wound healing on mice, effectively promoted the complete epithelialization of wound tissue, and generated new collagen fiber. The results of determination showed that the contents of polyphenols and flavonoids in the alcohol extract were higher than the alcohol extract of fruiting body. ConclusionThe alcohol extract of spore powder in Lasiosphaera Calvatia has active components in the treatment of wounds with good development prospect, and the medicinal components may be polyphenols and flavonoids.

9.
Article in Chinese | WPRIM | ID: wpr-940369

ABSTRACT

Jichuanjian, from the Jing Yue’s Collected Works by Zhang Jingyue, a famous doctor of Ming Dynasty, is composed of Angelica, Achyranthes bidentata, Cistanche deserticola, Alismatis Rhizoma, Shengma and Fructus Aurantii. It is one of the first 100 classic prescriptions published by the National Administration of Traditional Chinese Medicine. The original book states that where the disease is related to deficiency, with impacted stool, medicines such as Xiaohuang prescription cannot be used. If the treatment is needed, Jichuanjian should be applied. Through the textual research of ancient and modern literature, it is found that the efficacy of Jichuanjian has changed from ancient to modern times. In the medical books of the Ming and Qing Dynasties and the modern ones, the treatment is mostly carried out based on the constipation due to deficiency recorded in the original book, while in contemporary times, the treatment of Jichuanjian focuses on kidney (yang)-deficiency constipation and yin-deficiency constipation. Especially since modern times, Jichuanjian is frequently used to treat kidney-deficiency constipation according to the description in the planning textbooks of traditional Chinese medicine colleges and universities, which causes doubts among the authors. To actively respond to the call of General Secretary Xi Jinping that we should strengthen the sorting and excavation of the essence of classical medical books, this paper analyzed and demonstrated the original text and the drug composition, traceability and application by future generations of Jichuanjian based on the Jing Yue’s Collected Works. It was concluded that Jichuanjian was prepared for the treatment of constipation due to weak constitution, fluid deficiency, and emergent purgation, aiming for constipation due to weak constitution and fluid consumption rather than kidney-deficiency constipation described in modern textbooks. As Jichuanjian was developed for similar syndromes, it was reasonable that it was understood by later generations of doctors from the perspective of liver and kidney as well as earth dampness and wood stagnation, which however remains to be further explored and verified in clinic. By combing and discussing the efficacy of Jichuanjian, this paper could provide theoretical basis for the clinical application and modern development of Jichuanjian.

10.
Article in Chinese | WPRIM | ID: wpr-940354

ABSTRACT

ObjectiveTo study the effect of Longshengzhi capsules on the cognitive function of vascular dementia (VD) rats and reveal the underlying mechanism. MethodA VD rat model was established by permanent ligation of bilateral common carotid arteries. The model rats were randomly assigned into the model group (normal saline for gavage), Hydergine (0.54 mg·kg-1) group, and high-, medium-, and low-dose (2.16, 1.08, and 0.54 g·kg-1, respectively) Longshengzhi capsules groups, with 15 rats each group. Additionally, a sham group (normal saline for gavage) was designed in this study. Morris water maze test was conducted in the last week. Hematoxylin-eosin (HE) staining was employed for observation of the pathological changes in the hippocampal area of rat brain. Serum oxidative stress indicators including superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione peroxidase (GSH-Px) were examined. The levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) were determined by enzyme-linked immunosorbent assay (ELISA). The expression levels of B cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax), protein kinase B (Akt), p-Akt, nuclear factor-κB (NF-κB), intercellular cell adhesion molecule-1 (ICAM-1), and matrix metallopeptidase-9 (MMP-9) were determined by Western blot. ResultMorris water maze results showed that compared with sham operation group, the escape latency of rats in model group was significantly prolonged, and the number of crossing platform was significantly reduced. Compared with model group, the time of escape latency in Longshengzhi capsules high- and medium-dose groups was significantly shortened, and the number of crossing platform was significantly increased. Compared with sham operation group, SOD and GSH-Px levels in model group were significantly decreased (P<0.05, P<0.01), MDA levels were significantly increased (P<0.05), TNF-α, IL-6 and IL-1β levels were significantly increased (P<0.01). Compared with model group, the level of SOD in serum of Longshengzhi capsules high-dose group was significantly increased (P<0.05), the level of GSH-Px in serum of Longshengzhi capsules high-, medium- and low-dose groups was significantly increased (P<0.05, P<0.01), and the level of MDA was significantly decreased (P<0.05). TNF-α, IL-6 and IL-1β levels were significantly decreased (P<0.05, P<0.01). HE staining showed that Longshengzhi capsules could improve pathological damage in hippocampus of VD rats. Western blot results showed that compared with sham operation group, the protein expressions of Bax, NF-κB, MMP-9 and ICAM-1 in model group were significantly increased (P<0.01), and the protein expressions of Bcl-2, Akt and p-Akt were significantly decreased (P<0.05, P<0.01). The protein expression of Bax, NF-κB, MMP-9 and ICAM-1 in Longshengzhi capsules high-, medium- and low-dose groups was significantly decreased (P<0.05, P<0.01), and the protein expression of Bcl-2 was significantly increased (P<0.05, P<0.01). The protein expression of Akt in Longshengzhi capsules high- and medium-dose groups was significantly increased (P<0.05). The expression of p-Akt protein in Longshengzhi capsules high-dose group was significantly increased (P<0.01). ConclusionLongshengzhi capsules can improve the cognitive function of VD rats, and its mechanism may be related to the inhibition of oxidative stress, inflammatory response and apoptosis. ConclusionLongshengzhi capsules can improve the cognitive function of VD rats by inhibiting oxidative stress, inflammatory reaction, and neuronal apoptosis.

11.
Article in Chinese | WPRIM | ID: wpr-940346

ABSTRACT

ObjectiveTo study the effect and mechanism of Wuwei Xiaoduyin in treating rat renal mesangial cells (HBZY-1) induced by lipopolysaccharide (LPS) through the nuclear factor-κB (NF-κB) signaling pathway. MethodRat HBZY-1 cells were randomly assigned into the normal group, model group, benazepril (50 μmol·L-1) group, and high- and low-dose (2.75 and 0.69 g·kg-1) Wuwei Xiaoduyin groups. The normal group, model group, and benazepril group were treated with 10% normal rat serum, and the Wuwei Xiaoduyin groups with 10% medicated serum. Except the normal group, the other four groups were treated with LPS (100 ng·mL-1) for modeling in vitro. The changes of cell morphology were observed under optical microscope. The expression of NF-κB p65 was detected by immunofluorescence (IF) method. Methyl thiazolyl tetrazolium (MTT) colorimetry was employed to detect cytotoxicity and cell proliferation. The levels of interleukin-1β (IL-1β), intercellular adhesion molecule-1 (ICAM-1), laminin (LN), and fibronectin (FN) in cell supernatant were determined by enzyme-linked immunosorbent assay (ELISA). The mRNA levels of IL-1β, FN, and NF-κB p65 were measured by real-time fluorescence quantitative PCR. The protein levels of phosphorylated inhibitor of NF-κB kinase β (p-IKKβ), phosphorylated NF-κB inhibitor (p-IκBα), and NF-κB p65 were determined by Western blot. ResultCompared with the normal group, the modeling increased cell proliferation (P<0.01), elevated the levels of IL-1β, ICAM-1, LN, and FN in cell supernatant (P<0.01), and up-regulated the mRNA levels of IL-1β, FN, and NF-κB p65 (P<0.01) and the protein levels of p-IKKβ, p-IκBα, and NF-κB p65 (P<0.01). Such changes were recovered by benazepril and Wuwei Xiaoduyin (P<0.05, P<0.01). ConclusionWuwei Xiaoduyin can mitigate the inflammatory injury of renal mesangial cells induced by LPS by inhibiting the NF-κB signaling pathway.

12.
Article in Chinese | WPRIM | ID: wpr-940283

ABSTRACT

ObjectiveTo observe the effect of Linggui Zhugantang (LG) on the blood-brain barrier (BBB) model of Alzheimer's disease (AD) in vitro and to explore the mechanism of LG in repairing the BBB injury in AD. MethodA total of 50 male SPF rats were randomized into five groups: high-dose (4.8 g·kg-1), medium-dose (2.4 g·kg-1), and low-dose (1.2 g·kg-1) LG groups, western medicine (0.5 g·kg-1 donepezil hydrochloride) group, and normal group (normal saline of equivalent volume). They received (ig) corresponding drugs twice a day for 7 d. Drug-containing serum was respectively collected from the abdominal aorta 1 h after the last administration. The BBB injury of AD in vitro was induced with the cell co-culture method, and 6 groups were designed: normal group, model group, high-, medium-, and low-dose LG groups, and western medicine group. The model group was added with 100 μL amyloid β1-42 (Aβ1-42, final concentration: 5 μmol·L-1), and high-dose, medium-dose, and low-dose LG groups and the western medicine group were added with corresponding 10% drug-containing serum in addition to the 100 μL Aβ1-42 (final concentration: 5 μmol·L-1). Cell survival rate was detected by methyl thiazolyl tetrazolium (MTT) assay, expression of BBB-related skeleton proteins (claudin-5, ZO-1, occludin), matrix metalloproteinase-2 (MMP-2), and matrix metalloproteinase-9 (MMP-9) by Western blot, and content of inflammatory factors interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) by enzyme-linked immunosorbent assay (ELISA). BBB Aβ transporter low-density lipoprotein receptor-related protein 1 (LRP-1) and advanced glycation end product receptor (RAGE) at different time points in high-dose, medium-dose, and low-dose LG groups were determined by Real-time PCR and Western blot. ResultCell survival rate of the model group was lower than that of the normal group (P<0.05) and the survival rates of the western medicine group and high-dose LG group was higher than that in the model group (P<0.05). The skeleton proteins were down-regulated and MMP-2 and MMP-9 were up-regulated in the model group compared with those in the normal group (P<0.05). The expression of skeleton proteins was higher (P<0.05) and that of MMP-2 and MMP-9 was lower (P<0.05) in the western medicine group and high-dose LG group than in the model group. Compared with the model group, only the medium-dose LG group showed the up-regulation (P<0.05) of claudin-5 (P<0.05) and the decrease (P<0.05) of MMP-2. IL-1β, IL-6, and TNF-α in the model group were up-regulated (P<0.05) compared with those in the normal group, and those inflammatory factors in the western medicine group and high-dose and medium-dose LG groups were lower (P<0.05) than those in the model group. LRP-1 expression was up-regulated and RAGE expression was down-regulated at 3 h compared with those at 0 h (P<0.05), while the expression of the two became stable at 6, 12, 24, 36 h. At 3 h, LRP-1 expression was down-regulated and RAGE expression was up-regulated in model group compared with those in the normal group at 3 h (P<0.05). Moreover, the LRP-1 content was higher and RAGE content was lower in the western medicine group and high-dose LG group than in the model group. ConclusionLG can repair the BBB injury in vitro by inhibiting the expression of inflammatory factors and MMP-2, MMP-9, promoting the expression of skeletal proteins, and regulating the balance of transporters.

13.
Acta Pharmaceutica Sinica B ; (6): 2950-2962, 2022.
Article in English | WPRIM | ID: wpr-939924

ABSTRACT

Lipid nanoparticle (LNP) is commonly used to deliver mRNA vaccines. Currently, LNP optimization primarily relies on screening ionizable lipids by traditional experiments which consumes intensive cost and time. Current study attempts to apply computational methods to accelerate the LNP development for mRNA vaccines. Firstly, 325 data samples of mRNA vaccine LNP formulations with IgG titer were collected. The machine learning algorithm, lightGBM, was used to build a prediction model with good performance (R 2 > 0.87). More importantly, the critical substructures of ionizable lipids in LNPs were identified by the algorithm, which well agreed with published results. The animal experimental results showed that LNP using DLin-MC3-DMA (MC3) as ionizable lipid with an N/P ratio at 6:1 induced higher efficiency in mice than LNP with SM-102, which was consistent with the model prediction. Molecular dynamic modeling further investigated the molecular mechanism of LNPs used in the experiment. The result showed that the lipid molecules aggregated to form LNPs, and mRNA molecules twined around the LNPs. In summary, the machine learning predictive model for LNP-based mRNA vaccines was first developed, validated by experiments, and further integrated with molecular modeling. The prediction model can be used for virtual screening of LNP formulations in the future.

14.
Article in English | WPRIM | ID: wpr-939819

ABSTRACT

Although the coronavirus disease 2019 (COVID-19) epidemic is still ongoing, vaccination rates are rising slowly and related treatments and drugs are being developed. At the same time, there is increasing evidence of preexisting immunity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in humans, mainly consisting of preexisting antibodies and immune cells (including T cells and B cells). The presence of these antibodies is mainly due to the seasonal prevalence of four common coronavirus types, especially OC43 and HKU1. The accumulated relevant evidence has suggested that the target of antibodies is mainly the S2 subunit of S protein, followed by evolutionary conservative regions such as the nucleocapsid (N) protein. Additionally, preexisting memory T and B cells are also present in the population. Preexisting antibodies can help the body protect against SARS-CoV-2 infection, reduce the severity of COVID-19, and rapidly increase the immune response post-infection. These multiple effects can directly affect disease progression and even the likelihood of death in certain individuals. Besides the positive effects, preexisting immunity may also have negative consequences, such as antibody-dependent enhancement (ADE) and original antigenic sin (OAS), the prevalence of which needs to be further established. In the future, more research should be focused on evaluating the role of preexisting immunity in COVID-19 outcomes, adopting appropriate policies and strategies for fighting the pandemic, and vaccine development that considers preexisting immunity.


Subject(s)
COVID-19 , Humans , Pandemics , SARS-CoV-2 , Seasons , Spike Glycoprotein, Coronavirus
15.
Article in Chinese | WPRIM | ID: wpr-939640

ABSTRACT

OBJECTIVES@#To study the clinical application effect of "kindergarten effect" in radiotherapy for children with tumor based on the psychology of preschool children aged 3-5 years.@*METHODS@#A total of 30 children, aged 3-5 years, who were admitted to the Department of Radiotherapy, Tianjin Medical University Cancer Institute and Hospital, from January 2020 to August 2021 were enrolled in this prospective study. The children were randomly divided into a control group and a test group, with 15 children in each group. The children in the test group were treated in "kindergarten mode", i.e., all children were treated together at a specified time and left together after all children completed treatment. Those in the control group were treated alternately with adult patients according to the treatment time based on the type of radiotherapy fixation device. The treatment compliance was evaluated for both groups, and the two groups were compared in terms of the setup errors in the superior-inferior (SI), left-right (LR), and anterior-posterior (AP) directions.@*RESULTS@#Compared with the control group, the test group showed a significantly shorter time for finishing the treatment (P<0.05) and a significantly lower proportion of children with treatment interruption (P<0.05). Compared with the control group, the test group showed smaller mean errors in the SI, LR and AP directions after image-guided radiotherapy, with significant differences in the mean errors in the SI and LR directions (P<0.05).@*CONCLUSIONS@#With the application of the "kindergarten effect", most children can actively cooperate in radiotherapy, and it can also improve the accuracy and repeatability of positioning and help to achieve the desired treatment outcome.


Subject(s)
Adult , Humans , Neoplasms/radiotherapy , Prospective Studies , Radiotherapy Planning, Computer-Assisted
16.
Article in English | WPRIM | ID: wpr-939600

ABSTRACT

Objective@#To analyze the prevalence of dry and wet age-related macular degeneration (AMD) in patients with diabetes, hypertension and hyperlipidemia, and to analyze the risk factors for AMD.@*Methods@#A population-based cross-sectional epidemiologic study was conducted involving 14,440 individuals. We assessed the prevalence of dry and wet AMD in diabetic and non-diabetic subjects and analyzed the risk factors for AMD.@*Results@#The prevalence of wet AMD in diabetic and non-diabetic patients was 0.3% and 0.5%, respectively, and the prevalence of dry AMD was 17% and 16.4%, respectively. The prevalence of wet AMD in healthy, hypertensive, hyperlipidemic, and hypertensive/hyperlipidemic populations was 0.5%, 0.3%, 0.2%, and 0.7%, respectively. The prevalence of dry AMD in healthy, hypertensive, hyperlipidemic, and hypertensive/hyperlipidemic populations was 16.6%, 16.2%, 15.2%, and 17.2%, respectively. Age, sex, body mass index, and use of hypoglycemic drugs or lowering blood pressure drugs were corrected in the risk factor analysis of AMD. Diabetes, diabetes/hypertension, diabetes/hyperlipidemia, and diabetes/hypertension/hyperlipidemia were analyzed. None of the factors analyzed in the current study increased the risk for the onset of AMD.@*Conclusion@#There was no significant difference in the prevalence of wet and dry AMD among diabetic and non-diabetic subjects. Similarly, there was no significant difference in the prevalence of wet and dry AMD among subjects with hypertension and hyperlipidemia. Diabetes co-existing with hypertension and hyperlipidemia were not shown to be risk factors for the onset of dry AMD.


Subject(s)
Cross-Sectional Studies , Diabetes Mellitus/epidemiology , Humans , Hyperlipidemias/epidemiology , Hypertension/epidemiology , Macular Degeneration/etiology , Risk Factors
17.
Article in Chinese | WPRIM | ID: wpr-936061

ABSTRACT

Objective: The study aimed to investigate the safety and feasibility of intrathoracic modified overlap method in laparoscopic radical resection of Siewert type II adenocarcinoma of the esophagogastric junction (AEG). Methods: A descriptive case series study was conducted. The clinical data of 27 patients with Siewert type II AEG who underwent transthoracic single-port assisted laparoscopic total gastrectomy and intrathoracic modified overlap esophagojejunostomy in Guangdong Provincial Hospital of Chinese Medicine from May 2017 to December 2020 were retrospectively analyzed. The intrathoracic modified overlap esophagojejunostomy was performed as follows: (1) The Roux-en-Y loop was made; (2) The jejunum side was prepared extraperitoneal for overlap anastomosis; (3) The esophagus side was prepared intraperitoneal for overlap anastomosis; (4) The overlap esophagojejunostomy was performed; (5) The common outlet was closed after confirmation of anastomosis integrity without bleeding; (6) A thoracic drainage tube was inserted into the thoracic hole with the diaphragm incision closed. The intraoperative and postoperative results were reviewed. Results: All 27 patients were successfully operated, without mortality or conversion to laparotomy. The operative time, digestive tract reconstruction time and esophageal-jejunal anastomosis time were (327.5±102.0) minute, 50 (28-62) minute and (29.0±7.4) minute, respectively. The blood loss was 100 (20-150) ml. The postoperative time to flatus and postoperative hospital stay were (4.7±3.7) days and 9(6-73) days, respectively. Three patients (11.1%) developed postoperative grade III complications according to the Clavien-Dindo classification, including 1 case of anastomotic fistula with empyema, 1 case of pleural effusion and 1 case of pancreatic fistula, all of whom were cured by puncture drainage and anti-infective therapy. Conclusions: The intrathoracic modified overlap esophagojejunostomy is safe and feasible in laparoscopic radical resection of Siewert type II AEG.


Subject(s)
Adenocarcinoma/surgery , Anastomosis, Surgical , Esophagogastric Junction/surgery , Feasibility Studies , Gastrectomy/methods , Humans , Laparoscopy/methods , Retrospective Studies , Stomach Neoplasms/pathology
18.
Chinese Journal of Burns ; (6): 363-368, 2022.
Article in Chinese | WPRIM | ID: wpr-936019

ABSTRACT

Objective: To investigate the clinical effects of en bloc resection and debridement combined with gluteus maximus muscle flap in the treatment of ischial tubercle pressure ulcer complicated with chronic osteomyelitis. Methods: A retrospective observational study was conducted. From May 2018 to February 2020, 8 patients with pressure ulcers on the ischial tuberosity combined with chronic osteomyelitis who met the inclusion criteria were admitted to Fuyang Minsheng Hospital, including 5 males and 3 females, aged 38-69 years, with unilateral lesions in 6 patients and bilateral lesions in 2 patients. According to the anatomical classification of Cierny-Mader osteomyelitis, there were 6 patients (7 sides) with focal type, and 2 patients (3 sides) with diffuse type. The wound areas were 3 cm×2 cm to 12 cm×9 cm on admission. The pressure ulcer and chronic osteomyelitis lesions were completely removed by en bloc resection and debridement. The chronic infectious lesions were transformed into sterile incisions like fresh wounds by one surgical procedure, and the gluteus maximus muscle flaps with areas of 10 cm×6 cm to 15 cm×9 cm were excised to transfer and fill the ineffective cavity. The wounds of 5 patients were sutured directly, and the wounds of 3 patients were closed by local flap transfer. The intraoperative blood loss volume and blood transfusion, and length of hospital stay of patients were recorded. The incision healing and flap survival of patients were observed after operation. The recurrence of pressure ulcer and osteomyelitis, the appearance of the affected area, and the secondary dysfunction and deformity of the muscle flap donor site of patients were observed during followed up. Results: The intraoperative blood loss volume of the 8 patients was 220 to 900 (430±150) mL; 5 patients received intraoperative blood transfusion, of which 2 patients received 3 U suspended red blood cells and 3 patients received 2 U suspended red blood cells. The length of hospital stay was 18 to 29 (23.5±2.0) d for the 8 patients. In this group of patients, the incisions of 7 patients healed, while in one case, the incision suture was torn off during turning over and healed after secondary suture. The flaps survived well in 3 patients who underwent local flap transfer. During the follow-up period of 6-20 months, no recurrence of pressure ulcer or osteomyelitis occurred in 8 patients, the affected part had skin with good texture, mild pigmentation, and no sinus tract formation, and no secondary dysfunction or deformity occurred in the donor site. Conclusions: The en bloc resection and debridement combined with gluteus maximus muscle flap has good clinical effects on ischial tubercle pressure ulcer complicated with chronic osteomyelitis. Neither pressure ulcer nor osteomyelitis recurs post operation. The skin texture and appearance of the affected area are good, and the donor site has no secondary dysfunction or deformity.


Subject(s)
Blood Loss, Surgical , Debridement , Female , Humans , Male , Muscles/surgery , Osteomyelitis/surgery , Perforator Flap , Pressure Ulcer/surgery , Reconstructive Surgical Procedures , Skin Transplantation , Soft Tissue Injuries/surgery , Treatment Outcome
19.
Chinese Journal of Stomatology ; (12): 101-106, 2022.
Article in Chinese | WPRIM | ID: wpr-935836

ABSTRACT

In dental esthetic rehabilitation, patients pay great attention to the rehabilitative esthetic effect before teeth preparation, and this is also an important content of doctor-patient communication. Along with the development and combined application of intraoral scan, three-dimensional (3D) face scan, digital design, numerical control machining and 3D printing technology, digital technology is gradually applied to the virtual simulated design before irreversible operation in dental esthetic rehabilitation. Digital technology can be used in dentistry to simulate the esthetic outcome in advance, to assist communication among the dentists, patients and dental technicians, and to realize satisfactory outcome in the final restorations precisely, which, as a result, increases the clinical satisfaction. This review focuses on the application of digital virtual simulated design technology in dental esthetic rehabilitation, analyzes the current research development, deficiency and future prospects, so as to provide guidance for clinical diagnosis and treatment.


Subject(s)
Computer-Aided Design , Esthetics, Dental , Face , Humans , Printing, Three-Dimensional , Tooth Preparation
20.
Article in Chinese | WPRIM | ID: wpr-935782

ABSTRACT

Objective: To establish an ultrahigh performance liquid chromatography tandem mass spectrometry method for the determination of creatinine (Cre) and 2-thiothiazolidine-4-carboxylic acid (TTCA) in urine. Methods: In October 2020, the end-of-shift urine samples of the monitored subjects were taken, and the filtrate was prepared by centrifugation. After separated by ultra high performance liquid chromatography C18 column, acetonitrile and 0.2% acetic acid aqueous solution were used as mobile phases for gradient elution, the three quadrupole tandem mass spectrometry adopted an electrospray ion source (ESI) , the ion source temperature was 500 ℃ , and the air curtain gas flow rate was 31.4 L/min, qualitative and quantitative analysis of Cre and TTCA were carried out under the multiple reaction monitoring mode. Results: The linear range of Cre was 1.0-1 000.0 μg/L, the linear equation was y=947.3x-1605.6, and the correlation coefficient was 0.9994. The detection limit and the limit of quantitation were 0.3, 1.0 μg/L. When the addition concentrations were 50.0, 150.0 and 450.0 μg/L, the recovery rates were 92.8%-94.6% , the intra assay precisions were 3.6%-5.7% , and the inter assay precisions were 3.4%-5.4%. The linear range of TTCA was 0.1-200.0 μg/L, the linear equation was y=1164.7x-2243.9, and the correlation coefficient was 0.9991. The detection limit and the limit of quantitation were 0.03, 0.1 μg/L. When the addition concentrations were 10.0, 40.0 and 160.0 μg/L, the recovery rates were 90.8%-93.6%, the intra assay precisions were 4.6%-7.4%, and the inter assay precisions were 4.4%-6.9%. Conclusion: The sample pretreatment process of the ultra high performance liquid chromatography tandem mass spectrometry method for the determination of Cre and TTCA in urine is simple, and the continuous determination of Cre and TTCA in urine can be realized only by switching mass spectrometry parameters under the same chromatographic conditions, which is accurate and efficient, and each performance index of the method meets the determination requirements.


Subject(s)
Chromatography, High Pressure Liquid , Creatinine , Humans , Tandem Mass Spectrometry , Thiazolidines
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