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1.
China Medical Equipment ; (12): 82-86, 2023.
Article in Chinese | WPRIM | ID: wpr-1026408

ABSTRACT

Objective:To investigate the value of color Doppler ultrasound combined with serum miR-128-3p and DJ-1 in the diagnosis and clinical staging of bladder cancer.Methods:A total of 136 patients with suspected bladder cancer were selected and they were divided into bladder cancer group(56 cases)and benign lesion group(80 cases)according to the results of pathological examination.All patients underwent color Doppler ultrasound examination,and the levels of serum miR-128-3p and DJ-1 of them were measured.The receiver operating characteristic(ROC)curve was applied to analyze the diagnostic efficacy of color Doppler ultrasound examination combined with serum miR-128-3p and DJ-1 for bladder cancer and the clinical staging of that.Results:Compared with pathological results as the"gold standard",the sensitivities of color Doppler ultrasound in diagnosing bladder cancer and the clinical staging of that were respectively 75.00%and 80.49%,and the specificities of that for them were respectively 86.25%and 80.00%,and the accuracies of that for them were respectively 81.62%and 80.36%.Compared with benign lesion group,the serum miR-128-3p level decreased and DJ-1 level increased in bladder cancer group,the differences were statistically significant(t=8.842,t=8.844,P<0.05),respectively.In the bladder cancer group,the serum miR-128-3p level of infiltration type patients was lower than that of superficial type patients,and the serum DJ-1 level of them was higher than that of superficial type patients,and the differences were statistical significant(t=5.722,t=6.327,P<0.05),respectively.ROC curve results showed that the area under curve(AUC),sensitivity and specificity of color Doppler ultrasound combined with serum miR-128-3p and DJ-1 were respectively higher than those of single examination indicator in diagnosing bladder cancer and the clinical staging of that,and the differences were statistical significant(Z=4.028,Z=2.535,Z=3.386,Z=2.131,Z=2.137,Z=2.063,P<0.05).Conclusion:Color Doppler ultrasound combined with serum miR-128-3p and DJ-1 can improve the diagnostic efficacy for bladder cancer and the clinical staging of that,which has important clinical significance in diagnosing bladder cancer and judging the clinical staging of that.

2.
Article in Chinese | WPRIM | ID: wpr-905810

ABSTRACT

Objective:To analyze the epidemiological characteristics of 8 clusters of coronavirus disease 2019 (COVID-19) in Chenzhou City, and provide scientific basis for epidemic prevention and control. Methods:Descriptive epidemiological analysis was conducted for 8 COVID-19 clusters, comparing and analyzing the differences of infection rates among close contacts within and outside the family, and emphatically describing two typical cases. Results:8 COVID-19 clusters were reported in Chenzhou with a total of 31 cases from January to February, 2020. The main source of infection of the family index cases was Hubei Province. Cough symptoms were observed in 67.74% of the cases, followed by fever (54.84%). The infection rate of close contacts within the family (55.00%) was higher than that outside the family (2.56%), and the difference was statistically significant (χ2=28.177, P<0.001). The infection rate of spouse of the family index cases was 85.71%, higher than that of parents (77.78%), other family members (44.44%) and children (40.00%), and the difference was not statistically significant (χ2=6.004, P=0.120). Two typical cases suggested that both COVID-19 pre-symptomatic and asymptomatic patients have the potential to excrete the virus from the body and become sources of infection. Conclusion:Effective family prevention and control measures and early sampling and screening of people in key epidemic areas are conducive to early detection, early isolation and early treatment of infected people, so as to avoid the occurrence and spread of family clusters.

3.
National Journal of Andrology ; (12): 322-326, 2018.
Article in Chinese | WPRIM | ID: wpr-689757

ABSTRACT

<p><b>Objective</b>To evaluate the effect of cefoxitin prophylactic in reducing the incidence of severe infection after transrectal prostate biopsy (TRPB).</p><p><b>METHODS</b>This retrospective study included 155 cases of TRPB with a 5-day administration of oral levofloxacin at 200 mg bid (the control group) and another 167 cases with a 3-day administration of oral levofloxacin at the same dose plus intravenous cefoxitin at 2.0 g 2 hours before TRPB (the experimental group) according to the distribution characteristics of drug-resistance bacteria in our department. The patients of the control and experimental groups were aged (68.68 ± 8.12) and (68.72 ± 7.51) years, with PSA levels of (19.78 ± 21.57) and (21.15 ± 42.63) μg/L, involving (11.68 ± 1.44) and (11.77±1.02) biopsy cores, respectively. Comparisons were made between the two groups of patients in the incidence rate of severe infection, which was defined as lower urinary track symptoms plus the systemic inflammatory response syndrome (SIRS) within 7 days after TRPB.</p><p><b>RESULTS</b>The incidence rate of postoperative severe infection was significantly lower in the experimental group than in the control (0.6% [1/167] vs 5.8% [9/155], P < 0.05). Blood cultures revealed positive E-coli strains in 6 cases in the control group, including 5 ESBL-positive and 4 quinolone-resistant and amikacin-sensitive cases, all sensitive to cefoxitin, cefoperazone/sulbactam and imipenem. The only one case of severe infection was shown to be negative in blood culture.</p><p><b>CONCLUSIONS</b>Preoperative intravenous administration of cefoxitin according to the specific distribution characteristics of drug-resistance bacteria can significantly reduce the incidence of severe infection after TRPB.</p>


Subject(s)
Aged , Humans , Male , Middle Aged , Anti-Bacterial Agents , Therapeutic Uses , Biopsy , Methods , Cefoxitin , Therapeutic Uses , Drug Resistance, Bacterial , Escherichia coli , Escherichia coli Infections , Microbiology , Levofloxacin , Therapeutic Uses , Postoperative Complications , Blood , Prostate , Pathology , Retrospective Studies
4.
Article in English | WPRIM | ID: wpr-772976

ABSTRACT

Autoimmune diseases (ADs) arise from an abnormal immune response of the body against substances and tissues normally present in the body. More than a hundred of ADs have been described in the literature so far. Although their etiology remains largely unclear, various types of ADs tend to share more associated genes with other types of ADs than with non-AD types. Here we present GAAD, a gene and AD association database. In GAAD, we collected 44,762 associations between 49 ADs and 4249 genes from public databases and MEDLINE documents. We manually verified the associations to ensure the quality and credibility. We reconstructed and recapitulated the relationships among ADs using their shared genes, which further validated the quality of our data. We also provided a list of significantly co-occurring gene pairs among ADs; with embedded tools, users can query gene co-occurrences and construct customized co-occurrence network with genes of interest. To make GAAD more straightforward to experimental biologists and medical scientists, we extracted additional information describing the associations through text mining, including the putative diagnostic value of the associations, type and position of gene polymorphisms, expression changes of implicated genes, as well as the phenotypical consequences, and grouped the associations accordingly. GAAD is freely available at http://gaad.medgenius.info.


Subject(s)
Humans , Autoimmune Diseases , Genetics , Data Mining , Databases, Factual , Gene Regulatory Networks , Genetic Association Studies
5.
Article in Chinese | WPRIM | ID: wpr-280808

ABSTRACT

Contributions of Xin'an medical school and physicians to acupuncture theory were introduced in the article. Academic theories or characteristics of several physicians of Xin'an school such as YANG Xuan-cao, WU Kun, WANG Ji, WU Yi-ding, ZHENG Mei-jian and XU Chun-fu, et al were sorted out. Contributions of inheriting and illustrations on acupuncture theory were analyzed so as to expound its significance and value on modern acupucture clinic.


Subject(s)
Humans , Acupuncture , Education , History , Workforce , Acupuncture Therapy , History , China , History, Ancient , Physicians , History , Schools, Medical , History , Workforce
6.
Chinese Journal of Endemiology ; (6): 675-677, 2012.
Article in Chinese | WPRIM | ID: wpr-643164

ABSTRACT

Objective To understand and master the situation of residents consumption of iodized salt in Chenzhou city of Hunan province,to identify problems and take appropriate interventions to ensure the residents consumption of qualified iodized salt,and to provide a scientific basis for elimination of iodine deficiency disorders (IDD).Methods According to the National Iodized Salt Monitoring Program (Amendment) and the Evaluation Scheme for Elimination of Iodine Deficiency Disorders at the County Level,the monitoring counties,towns and villages were selected in Chenzhou city from 2008 to 2011,the content of iodine in salt was detected using direct titration.The data was analyzed by SPSS 17.0 and Excel 2003.Results A total of 12700 salt samples were tested from 2008 to 2011.The iodized salt coverage rate,the qualified rate of iodized salt,the consumption rate of qualified iodized salt and the rate of non-iodized salt was 99.19% (12597/12700),96.33% (12135/12597),95.55%(12135/12700) and 0.81% (103/12700),respectively.There were significant differences between each year from 2008 to 2011 (x2 =13.99、42.35、48.45、13.99,P all < 0.01).The coefficient of variation was 21.19%.The median and average of iodine content in salt samples was 32.2 mg/kg and 31.9 mg/kg,respectively.Compared with the median and average of iodized salt content,there was no significant difference between each year from 2008 to 2011 (t =2.941,P > 0.05),while there was significant difference among the 11 counties(t =2.983,P < 0.05).Conclusions The goal of eliminating IDD has realized in the city of Chenzhou since 2010.To consolidate the IDD control results,surveillance should be strengthened in future.

7.
Chinese Medical Journal ; (24): 284-290, 2011.
Article in English | WPRIM | ID: wpr-321453

ABSTRACT

<p><b>BACKGROUND</b>Capsular contracture has become the most common complication associated with breast implant. Transforming growth factor-beta (TGF-β) is well known for a prominent role in fibrotic diseases. Due to the critical role of TGF-β in pathogenesis of capsular formation, we utilized thermosensitive C/GP hydrogel to controlled release of TGF-β receptor kinase inhibitor (SD208) and investigated their effects on capsular contracture.</p><p><b>METHODS</b>In vitro degradation and drug release of C/GP hydrogel were performed. Twenty-four rabbits underwent subpanniculus implantation with 30 ml smooth silicone implants and were randomly divided into four groups as follows: Group 1 received saline solution; Group 2 received SD208; Group 3 received SD208-C/GP; Group 4 received C/GP. At 8 weeks, the samples of capsular tissues were analyzed by hematoxylin and eosin and immunohistological staining. The mRNA expression of collagen III and TGF-β1 was detected by RT-PCR assay.</p><p><b>RESULTS</b>C/GP hydrogel could be applied as an ideal drug delivery vehicle which supported the controlled release of SD208. SD208-C/GP treatment showed a significant reduction in capsule thickness with fewer vessels. The histological findings confirmed that the lower amounts of inflammatory cells and fibroblasts infiltrate in SD208-C/GP group. In contrast, typical capsules with more vessel predominance were developed in control group. We did not observe the same inhibitory effect of SD208 or C/GP treatment on capsular contracture. Moreover, SD208-C/GP therapy yielded an evident down-regulation of collagen III and TGF-β1 mRNA expression.</p><p><b>CONCLUSIONS</b>This study demonstrated that controlled release of TGF-β receptor kinase inhibitor from thermosensitive C/GP hydrogel could significantly prevent capsule formation after mammary implants.</p>


Subject(s)
Animals , Rabbits , Breast Implantation , Chitosan , Chemistry , Glycerophosphates , Chemistry , Hydrogel, Polyethylene Glycol Dimethacrylate , Chemistry , Immunohistochemistry , Protein Kinase Inhibitors , Therapeutic Uses , Receptors, Transforming Growth Factor beta , Reverse Transcriptase Polymerase Chain Reaction
8.
Article in Chinese | WPRIM | ID: wpr-268636

ABSTRACT

<p><b>OBJECTIVE</b>To discuss the method for repairing the circular scar in the forearm.</p><p><b>METHODS</b>Large expander (400-600 ml) were implanted subcutaneously at the rear flank of bottom-waist in the way of overlapping or continued expansion. After expansion was completed, the horizontal opened omega-shaped flap was formed by advancement of expanded skin with the two pedicles at the two sides. Then the forearm was tunneled under flap with the wound covered by flap. The pedicles were cut off at the third stage.</p><p><b>RESULTS</b>From April 1998 to June 2009, 8 cases were treated with no flap necrosis. The patients were followed up for 1 to 3 years with good flap color and thickness. Flap sensory was partially recovered. Linear atrophic scar was left in the donor sites.</p><p><b>CONCLUSIONS</b>It is feasible to repair forearm circular scar by expanded double-pedicle omega-shaped flap at the rear flank of bottom-waist.</p>


Subject(s)
Adolescent , Adult , Female , Humans , Male , Young Adult , Cicatrix , General Surgery , Follow-Up Studies , Forearm , General Surgery , Surgical Flaps , Tissue Expansion , Treatment Outcome
9.
Chinese Journal of Surgery ; (12): 74-78, 2011.
Article in Chinese | WPRIM | ID: wpr-346352

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the minimally invasive efficacy and surgical outcome of full-endoscopic discectomy via interlaminar approach for lumbar disc herniation (LDH).</p><p><b>METHODS</b>From August 2008 to February 2009, 56 patients with lumbar disc herniation were retrospectively studied. The patients were divided into two groups according to the surgical methods. Full endoscopic discectomy (FED) group included 16 males and 12 females, the age was 20 - 51 years with a mean (36 ± 8) years, and the course of disease was 18 - 120 d with a mean (68 ± 26) days. There was L(5)-S(1) LDH in 22 and L(4-5) LDH in 6. Headlamp-assisted mini-open discectomy (HAMOD) group, there were 17 males and 11 females. The age was 17-53 years with an average age of (35 ± 9) years, the course of disease was 19 - 110 d with an average (66 ± 24) days, and the herniated disc located at L(5)-S(1) in 15 cases, and L(4-5) in 13 cases. Perioperative parameters (operation time, bleeding volume and length of hospital stay), complications and VAS of leg and back pain (preoperatively, 3 months postoperatively and final follow-up) were statistically analyzed.</p><p><b>RESULTS</b>All patients were followed up in both groups, and the average follow-up time of full endoscopic was 1.8 years, and headlamp assisted mini-open was 1.7 years. The average operation time in full endoscopic group was (71 ± 30) min and the headlamp group was (60 ± 12) min, which there was no statistical difference (P > 0.05). There was no measurable bleeding in full endoscopic group, and the headlamp group was (59 ± 10) ml. The average hospital days in full endoscopic group was (5.7 ± 1.4) days, and the headlamp group was (12.3 ± 3.0) days, there was statistically significant difference in both groups (P < 0.01). The complication rate in full endoscopic group was 7.1%, and in headlamp group was 10.7%, without statistical difference (P > 0.05). There was no recurrent case in either group. With regard to VAS of back pain and leg pain, statistically significant difference was found in each group between preoperatively and 3 months postoperatively, but not between 3 months postoperatively and at final follow-up. With regard to the final follow-up VAS, there was no statistical difference in leg pain between full endoscopic and headlamp group (P > 0.05). However, there was statistical significance in VAS back pain between the two groups (P < 0.01).</p><p><b>CONCLUSIONS</b>Compared to the headlamp assisted mini-open technique, the full-endoscopic interlaminar approach for the surgical treatment of lumbar disc herniation can achieve similar clinical outcomes with advantage of less iatrogenic trauma and sooner rehabilitation.</p>


Subject(s)
Adolescent , Adult , Female , Humans , Male , Middle Aged , Young Adult , Diskectomy , Methods , Endoscopy , Follow-Up Studies , Intervertebral Disc Displacement , General Surgery , Lumbar Vertebrae , Retrospective Studies
10.
Article in Chinese | WPRIM | ID: wpr-243342

ABSTRACT

CD4+ T cells mainly interact with Sphingosine 1-phosphate (S1P) to regulate immune function through Sphingosine 1-phosphate receptor 1 (S1P1). This study was aimed to investigate the effects of recombinant human granulocyte-colony-stimulating factor (rhG-CSF) mobilization on S1P1 expression in CD4+ T cells of donor's peripheral blood. The CD4+T cells of peripheral blood were isolated by magnetic beads from 17 allo-hematopoietic stem cell transplantation (allo-HSCT) donors before and at fourth day of mobilization with rhG-CSF. The S1P1 expression was detected by real time quantitative PCR in the RNA extracted from CD4+ T cells collected before and after rhG-CSF mobilization. The results showed that the expression of S1P1 was found in CD4+ cells before and after rhG-CSF mobilization, but the expression level of SIP1 in CD4+ cells after rhG-CSF mobilization was significantly lower than that before rhG-CSF mobilization (p<0.01). It is concluded that the mobilization with rhG-CSF obviously down-regulates the expression of S1P1 in CD4+ T cells of donor's peripheral blood.


Subject(s)
Humans , CD4-Positive T-Lymphocytes , Metabolism , Granulocyte Colony-Stimulating Factor , Pharmacology , Hematopoietic Stem Cell Mobilization , Methods , Hematopoietic Stem Cell Transplantation , Receptors, Lysosphingolipid , Metabolism , Recombinant Proteins
11.
Article in Chinese | WPRIM | ID: wpr-297845

ABSTRACT

<p><b>OBJECTIVE</b>To explore the external fixation for treatment of supracondylar femoral fractures after total knee replacement.</p><p><b>METHODS</b>From June 2005 to July 2007, 7 cases of supracondylar femoral fracture after total knee replacement were treated with external fixation included 4 males and 3 females with an average age of 71 years ranging from 55 to 85 years. The fracture healing were observed and the knee function were evaluated by the HSS scoring.</p><p><b>RESULTS</b>All patients were followed-up for 6 to 23 months with an average of 12.5 months. The fracture healing time was from 6 to 12 weeks after operation (averaged 8.5 weeks). During the followed-up period, there were no infection and loosening, only one case occurred nail crossing delayed healing of skin. The HSS knee score was (60.6 +/- 16.0) before treatment and (77.6 +/- 11.6) after treatment according to HSS knee score criteria, the results were excellent in 2 cases, good in 4, and fair in 1.</p><p><b>CONCLUSION</b>Application of external fixation for treatment of supracondylar femoral fracture after total knee replacement, especially in poor physical condition, high age patients is a more appropriate treatment.</p>


Subject(s)
Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Arthroplasty, Replacement, Knee , External Fixators , Femoral Fractures , General Surgery , Fracture Fixation , Methods , Postoperative Complications , General Surgery , Recovery of Function , Treatment Outcome
12.
Article in Chinese | WPRIM | ID: wpr-257943

ABSTRACT

<p><b>OBJECTIVE</b>To compare therapeutic effects of acupoint application of Chinese medicine and routine acupuncture on bronchial asthma.</p><p><b>METHODS</b>One hundred cases were randomly divided into an observation group and a control group, 50 cases in each group. The observation group was treated with application of Chinese medicine at Dazhui (GV 14), Feishu (BL 13), Gaohuang (BL 43), Danzhong (CV 17), Qihai (CV 6) in the dog days; and the control group was treated with acupuncture at Zhongfu (LU 1), Tiantu (CV 22), Danzhong (CV 17), Dingchuan (EX-B 1), Fenglong (ST 40). The long-term therapeutic effects were observed.</p><p><b>RESULTS</b>The total effective rate was 94.0% in the observation group and 76.0% in the control group with a significant difference between the two groups (P < 0.05).</p><p><b>CONCLUSION</b>The therapeutic effect of acupoint application of Chinese medicine in the dog days on bronchial asthma is significantly superior to that of routine acupuncture.</p>


Subject(s)
Adolescent , Adult , Aged , Child , Female , Humans , Male , Middle Aged , Young Adult , Acupuncture Points , Acupuncture Therapy , Methods , Asthma , Pathology , Therapeutics , Medicine, Chinese Traditional , Methods , Time Factors , Treatment Outcome
13.
Article in English | WPRIM | ID: wpr-334052

ABSTRACT

This study was aimed to clone human intercellular adhesion molecule-1 (ICAM-1) gene, to transfect the constructed eukaryotic expression vector ICAM-1-GFP into CHO cells, as well as to detect ICAM-1-GFP expression in CHO cells binding with Molt-4 cells. ICAM-1 cDNA gene was amplified by RT-PCR and inserted in PMD(18)-T vector. Then ICAM-1 cDNA from pMD18-ICAM-1 vector was subcloned into eukaryotic expression vector pEGFP-C1 to construct recombinant ICAM-1-pEGFP-C1 vector. Restriction analysis and DNA sequencing were used to confirm the recombinant vector. After stable transfection of CHO-K1 cells with the recombinant vector, the expression and subcellular localization of ICAM-1-GFP were detected by RT-PCR, flow cytometry and fluorescence microscopy. The function of ICAM-1-GFP fusion protein was assessed by the binding of ICAM-1-GFP/CHO cells to Molt-4 cells. The results showed that 1622 bp full-length ICAM-1 cDNA obtained and was successfully ligated with pMD(18)-T-vector, subcloned to construct recombinant ICAM-1-pEGFP-C1 vector. Restriction analysis and DNA sequencing indicated that recombinant ICAM-1-GFP was successfully constructed and ICAM-1-GFP was expressed stably in CHO cells. ICAM-1-GFP expression was only observed in the cytoplasm of ICAM-1-GFP/CHO cells by fluorescence microscopy. The ICAM-1-GFP/CHO cells were bound to PMA-treated Molt-4 cells. The expression of MEM-148 was very weak in PMA-treated Molt-4 cells. It is concluded that the ICAM-1-GFP eukaryotic expression vector has been constructed successfully and expresses stably in CHO cells. PMA can increase the binding of Molt-4 cells to ICAM-1-GFP/CHO cells by inducing specialized form of ICAM-1 clustering.


Subject(s)
Animals , Cricetinae , Humans , CHO Cells , Cricetulus , DNA, Complementary , Genetics , Genetic Vectors , Green Fluorescent Proteins , Genetics , Intercellular Adhesion Molecule-1 , Genetics , Recombinant Proteins , Genetics , Transfection
14.
Article in Chinese | WPRIM | ID: wpr-337528

ABSTRACT

<p><b>OBJECTIVE</b>To observe changes in activity and proliferation of cultured Schwann cells under the high glucose condition and effect of allyl glycoside extracted from Herba Rhodiolae (AG) on these changes for exploring the possible mechanism of diabetic peripheral nerve injury and the pharmacologic action of AG on them.</p><p><b>METHODS</b>Adopting refined Brokes method, Schwann cells were isolated from the sciatic nerve tissue of newborn Wistar rats and purified. Their activity and proliferative capability were determined using XTT method and 3H-TdR incorporative method respectively.</p><p><b>RESULTS</b>High glucose showed marked inhibitory effect on both activity and proliferative capability of Schwann cells, and the inhibition could be markedly improved by AG.</p><p><b>CONCLUSION</b>Diabetic peripheral nerve injury is possibly related to inhibitory effect of high glucose on activity and proliferative capability of Schwann cells. Effect of AG in improving these inhibitory changes provides the experiment basis for clinical use of traditional Chinese medicine, Herba Rhodiolae.</p>


Subject(s)
Animals , Male , Rats , Animals, Newborn , Cell Proliferation , Cells, Cultured , Diabetic Neuropathies , Drugs, Chinese Herbal , Pharmacology , Glucose , Pharmacology , Glycosides , Pharmacology , Plants, Medicinal , Chemistry , Rats, Wistar , Schwann Cells , Cell Biology , Sciatic Nerve , Cell Biology
15.
Article in Chinese | WPRIM | ID: wpr-325875

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the reconstruction of skin defect at frontal and temporal hair line.</p><p><b>METHODS</b>5 cases with skin defect at frontal and temporal hair line were treated with mastoid fasciocutaneous island flap pedicled with parietal branch of superficial temporal artery.</p><p><b>RESULTS</b>All the flaps survived completely. Hair grew up 5 - 7 days after operation, showing good reconstructed hair line.</p><p><b>CONCLUSIONS</b>Mastoid fasciocutaneous island flap is a reliable method for reconstruction of skin defect at frontal and temporal hair line.</p>


Subject(s)
Adult , Female , Humans , Male , Middle Aged , Carcinoma, Basal Cell , General Surgery , Carcinoma, Squamous Cell , General Surgery , Fascia , Transplantation , Forehead , Wounds and Injuries , Mastoid , General Surgery , Plastic Surgery Procedures , Skin , Wounds and Injuries , Skin Neoplasms , General Surgery , Skin Transplantation , Surgical Flaps
16.
Article in Chinese | WPRIM | ID: wpr-292855

ABSTRACT

<p><b>OBJECTIVE</b>To compare the differences between bone-length measurements on human head and to probe proportion relation of the position of head points.</p><p><b>METHODS</b>One hundred healthy adult volunteers, aged between 18-25 years, 50 males and 50 females, were randomly selected, and their height, body weight, the distances from Shenting (GV 24) to Toulinqi (GB 15), from Shenting to Touwei (ST 8), between bilateral Touwei (ST 8), between the two Mastoid, from Yintang (EX-HN 3) to front hairline, front hairline to Naohu (GV 17), Naohu to Fengfu (GV 16), Fengfu to the middle of rear hairline were measured respectively with standard measure instruments, and the proportion relation between bone-length measurement location and the point position were compared.</p><p><b>RESULTS</b>There was a significant difference between the distance of bilateral Touwei and the distance of the two Wangu (P<0.05). The proportion relation of Shenting-Toulinqi, Shenting-Touwei, bilateral Touwei distances basically conformed to the bone-length measurement location, while in the proportion relation of the distances of Yintang-front hairline, front hairline-Naohu, Naohu-Fengfu, Fengfu-the middle of rear hairline and the bone-length measurement location there were some differences.</p><p><b>CONCLUSION</b>Bilateral Touwei distance is not same as the bone-length measurements between two Mastoid, and Toulinqi can be located at the middle point of the connecting line of Shenting and Touwei. There is a certain deviation between marker location of Naohu, Fengfu and other points on body surface and the bone-length measurements.</p>


Subject(s)
Adolescent , Adult , Female , Humans , Male , Acupuncture Points , Bone and Bones , Head , Medicine, Chinese Traditional
17.
Article in Chinese | WPRIM | ID: wpr-293403

ABSTRACT

<p><b>OBJECTIVE</b>To observe the changes in the learning and memory functions and the hippocampal expression of phosphorylated cAMP response element-binding protein (pCREB) in rats with status epilepticus and generalized nonconvulsive status epilepticus.</p><p><b>METHODS</b>Status epilepticus (SE) and generalized nonconvulsive status epilepticus (GNCSE) was induced by pentylenetetrazol kindling in SD rats, and the learning and memory function changes of the kindled rats were assessed by means of Morris water-maze test and Y-maze test with alternative electric stimulation. Immunocytochemistry was used for analysis pCREB protein expression in the hippocampus of the rats.</p><p><b>RESULTS</b>In Morris water-maze test, the rats with SE showed prolonged mean escape latency (P<0.05), shortened swimming time in the platform quadrant (P<0.05), and reduced number of times of platform crossing (P<0.05) in the short term after kindling. But these changes were reversed and became normal a month after the kindling (P>0.05). In the Y-maze test with alternative electric stimulation, the total error (TE) of SE rats increased significantly in the short term after epilepsy (P<0.05), but recovered the normal level a month after kindling (P>0.05). The GNCSE rats showed prolonged mean escape latency at only certain time periods (P<0.05) in the short term, but with swimming time in the platform quadrant and number of platform crossings similar to the control group (P>0.05). The short-term TE of GNCSE rats increased significantly (P<0.05), but in the long term, TE was similar to that in the control group (P>0.05). The expression of pCREB decreased significantly in SE group in comparison with the control group in the short term.</p><p><b>CONCLUSION</b>Epileptic seizures can lead to learning and memory function impairment in rats, and SE seems to cause greater impact than GNCSE on the learning and memory functions. pCREB might be involved in the pathophysiology of learning and memory deficit in epileptic rats.</p>


Subject(s)
Animals , Rats , CREB-Binding Protein , Metabolism , Hippocampus , Metabolism , Kindling, Neurologic , Maze Learning , Memory Disorders , Pentylenetetrazole , Rats, Sprague-Dawley , Status Epilepticus , Metabolism
18.
Article in Chinese | WPRIM | ID: wpr-324295

ABSTRACT

<p><b>OBJECTIVE</b>To study the characteristics of protein phosphorylation of p65, IkappaBalpha and IkappaBepsilon in lymphocytes of rats in the progress of aging and the the interventional effect of Epimedium flavonoids (EF).</p><p><b>METHOD</b>We chose the lymphocytes derived from SD rat spleen. We divided the SD rats into five groups i. e. 4 months (4 m), 27 months (27 m), the 27 m EF treated group (27 m + EF), and we also observed the whole interventional effect of PDTC (a NF-kappaB inhibitor) on old rat groups (27 m PDTC, 27 m PDTC + EF ) when IkappaBepsilon, IkappaBalpha were detected. Through the western-blotting analyses, we studied the entire characteristics and distinctions of phosphorylation expression in molecules related to NF-kappaB signal transduction pathway p65, IkappaBepsilon and IkappaBalpha in lymphocytes across the age spectrum of rats in aging. We also observed the whole interventional effect of EF on lymphocytes of old rats.</p><p><b>RESULT</b>With the increasing of age, the mean level of phosphorylation expressions of p65, IkappaBalpha and IkappaBepsilon in rat spleen lymphocytes decreased obviously, When inhibited NF-kappaB by PDTC, there was decreased evidently, while PDTC + EF can active NF-kappaB family and the above molecules were increased to a certain extent.</p><p><b>CONCLUSION</b>The phosphorylation expressions of p65, IkappaBalpha and IkappaBepsilon in rat spleen lymphocytes were not enouphe, EF have a strong effect to upregulated the expression of them during aging.</p>


Subject(s)
Animals , Humans , Male , Rats , Aging , Physiology , Blotting, Western , Epimedium , Chemistry , Flavonoids , Pharmacology , Lymphocytes , Metabolism , NF-kappa B , Metabolism , Neoplasm Proteins , Metabolism , Nucleocytoplasmic Transport Proteins , Metabolism , Phosphorylation , Rats, Sprague-Dawley
19.
Chinese Journal of Hematology ; (12): 115-118, 2007.
Article in Chinese | WPRIM | ID: wpr-328363

ABSTRACT

<p><b>OBJECTIVE</b>To investigate antitumor activity and molecular mechanism of deguelin to the human U937 leukaemia cells and to explore the mechanisms regulating cell cycle and nucleoporin 98 (Nup98) and nucleoporin 88 (Nup88) in vitro.</p><p><b>METHODS</b>The effects of deguelin on the growth of U937 cells were studied by MTT assay, and the cell cycle of U937 cells by a propidium iodide method. The localization of the nuclear pore complex protein Nup98 and Nup88 was checked by immunofluorescence and immunoelectron microscopy. The expressions of Nup98 and Nup88 in U937 cells were checked by flow cytometry (FCM) and Western blot respectively.</p><p><b>RESULTS</b>The proliferation of U937 cells was significantly inhibited in a time-dose dependent manner in deguelin-treated group with a 24 h IC50 value of 21.61 nmol/L and 36 h IC50 value of 17.07 nmol/L. U937 cells treated with deguelin showed reduction in the percentages of cells in G0/G1, whereas accumulation of cells in S and G2/M phase. The ratio of G1/G0 phase cells were 73.01%, 71.15%, 68.42%, 52.45%, 43.99% and 22.82%, and that of S phase cells were 17.18%, 16.30%, 18.09%, 27.56%, 31.21% and 46.85%, and that of G2/M phase cells were 9.75%, 12.31%, 13.09%, 18.99%, 24.83% and 27.79% at deguelin concentrations of 0, 5, 10, 20, 40, 80 nmol/L respectively. Nup88 and Nup98 were found on both the nuclear and cytoplasmic side of the U937 cells. The expression of Nup98 was up-regulated and Nup88 down-regulated in deguelin treated U937 cells.</p><p><b>CONCLUSION</b>Deguelin is able to inhibit the proliferation of U937 cells by regulating the cell cycle. The antitumor activity of deguelin was related to up-regulating the expression of Nup98 and down-regulating Nup88 protein.</p>


Subject(s)
Humans , Cell Cycle , Cell Proliferation , Nuclear Pore Complex Proteins , Metabolism , Rotenone , Pharmacology , U937 Cells
20.
Article in Chinese | WPRIM | ID: wpr-269117

ABSTRACT

<p><b>OBJECTIVE</b>To explore similarity and difference of connotation between Shen deficiency syndrome (SDS) and Shen-yang deficiency syndrome (SYDS) on the molecular level.</p><p><b>METHODS</b>The senescent SD rats and corticosterone-treated rats were adopted for models of SDS and SYDS respectively, their syndrome was differentiated according to the therapeutic efficacy of treatment with epimedium flavonoids (EF). The gene expression profiles of hypothalamas, pituitary, adrenal gland and lymphocytes (HPAT axis) were detected before and after EF treatment using gene chip provided by Affymetrix company.</p><p><b>RESULTS</b>As compared with the young rats, the ageing rats and corticosterone-treated rats showed a significant down-regulation in highly consistent pattern, of various neurotransmitters of HPAT axis firstly, followed with that of growth and sex hormone related genes. EF could reverse the above genes expression in both models, and for SYDS model rats, it could also significantly up-regulate the gene expressions of heat shock protein, cytochrome P450 and thyroid stimulating hormone (TSH).</p><p><b>CONCLUSION</b>Both SDS and SYDS model rats show connotation of Shen deficiency, and the substantial base of Shen-yang deficiency syndrome resides in the process of oxidative phosphorylation of energy metabolism accelerated by thyroid hormone.</p>


Subject(s)
Animals , Male , Rats , Adrenal Glands , Metabolism , Diagnosis, Differential , Epimedium , Chemistry , Flavonoids , Therapeutic Uses , Gene Expression Profiling , Hypothalamus , Metabolism , Lymphocytes , Metabolism , Medicine, Chinese Traditional , Oligonucleotide Array Sequence Analysis , Methods , Pituitary Gland , Metabolism , Rats, Sprague-Dawley , Syndrome , Time Factors , Yang Deficiency , Diagnosis , Drug Therapy , Genetics
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