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1.
China Pharmacy ; (12): 842-847, 2022.
Article in Chinese | WPRIM | ID: wpr-923191

ABSTRACT

OBJECTIVE To study the quality grade stand ard of the premature Forsythia suspensa . METHODS A total of 138 batches of premature F. suspensa were collected from the main producing areas of F. suspensa in China. According to 2020 edition of Chinese Pharmacopoeia ,the contents of impurities ,moisture,ethanol-soluble extract ,volatile oil ,forsythin and forsythoside A in the premature F. suspense were determined ,and the qualified samples were screened. AHP-PCA mixed weighting method was used to give comprehensive weight to the indicators (except for the limit of impurity ). The comprehensive score of the samples was calculated. The suggestions on the quality grade division of premature F. suspensa were put forward according to cluster analysis of K-mean value. RESULTS & CONCLUSIONS The contents of impurities ,moisture,ethanol-soluble extract ,volatile oil ,forsythin and forsythoside A in the premature F. suspense were 0-7.80%,1.60%-8.18%,13.13%-61.60%,0.21%-3.47%,0.02%-2.15% and 0.79%-14.04%,respectively;average contents of them were 1.24%,4.97%,34.88%,2.01%,0.42%,6.86%,respectively. Totally 47 batches of 138 batches were qualified in all indexes. It is suggested that the quality grade of the premature F. suspense can be divided into three grades :in first grade of F. suspense ,the contents of volatile oil ,forsythin,forsythoside A , ethanol-soluble extract and moisture were ≥2.40%,≥0.59%,≥8.34%,≥38.66% and ≤4.99%,respectively;in second grade of F. suspense ,the contents of above indicators were ≥2.26%,≥0.41%,≥7.47%,≥32.58% and ≤5.33%,respectively;in third grade of F. suspense ,the contents of above indicators were ≥2.15%,≥0.32%,≥4.60%,≥31.52% and≤7.23%,respectively.

2.
Chinese Journal of Endemiology ; (12): 674-678, 2021.
Article in Chinese | WPRIM | ID: wpr-909076

ABSTRACT

Objective:To understand the knowledge and behavior changes of pregnant women on iodine deficiency disorders (IDD) prevention and treatment in iodine deficiency areas in Anhui Province before and after the implementation of the intervention measures, and to provide a scientific basis for pregnant women's iodine nutrition improvement.Methods:From March to December 2018, from Lujiang County, an iodine deficiency area in Anhui Province, Lucheng and Nihe towns were selected as the survey sites. Relying on the township health centers, pregnant women in early pregnancy (≤12 weeks) were selected as the survey subjects, and long-term follow-up was conducted. The edible salt samples of pregnant women in early pregnancy were collected and salt iodine content was detected by direct titration method. The urine samples of pregnant women in the morning in early, middle (13 - 28 weeks) and late pregnancies (≥29 weeks) were collected, urinary iodine content was determined by arsenic-cerium catalytic spectrophotometry. Baseline questionnaire survey was conducted for pregnant women in early pregnancy, mainly including basic information, IDD prevention and treatment knowledge (pregnant women prone to iodine deficiency, the harm of iodine deficiency in pregnant women, suitable iodine supplement methods for pregnant women and foods with high iodine content), and the consumption frequency of iodine-rich foods. After the baseline survey, the knowledge publicity on IDD prevention and treatment was carried out in townships, and iodine-rich foods such as kelp and laver were recommended to supplement iodine. The intervention activities lasted for 6 months, and retrospective questionnaire survey was conducted on pregnant women in late pregnancy.Results:A total of 128 edible salt samples were collected from the families of pregnant women in early pregnancy, and the median salt iodine was 21.5 mg/kg. The iodized salt coverage rate was 99.2% (127/128), the qualified rate of iodized salt was 98.4% (125/127), and the consumption rate of qualified iodized salt was 97.7% (125/128). A total of 129, 95 and 70 urine samples were collected from pregnant women in early, middle and late pregnancies, the medians urinary iodine were 179.0, 185.5 and 189.7 μg/L, respectively, all of which were at the appropriate iodine level. The total awareness rates of IDD prevention and treatment before and after intervention were 22.4% (28/125) and 64.6% (82/127), respectively, and the difference was statistically significant (χ 2 = 45.538, P < 0.01). Compared with the awareness rates before the intervention, the awareness rates of the harm of iodine deficiency in pregnant women, suitable iodine supplement methods for pregnant women and foods with high iodine content were all higher after the intervention ( P < 0.01). There were statistically significant differences in the frequency of eating kelp, laver and other iodine-rich foods among pregnant women in early, middle and late pregnancies (χ 2 = 163.170, 102.373, P < 0.01). Before the intervention, 57 (45.2%) pregnant women had not eaten kelp, which decreased to 1 (0.8%) pregnant woman after the intervention. Before the intervention, 72 (57.1%) pregnant women had not eaten laver and other iodine-rich foods, which decreased to 7 (5.5%) pregnant women after the intervention. Conclusions:After the intervention, the awareness rate of IDD prevention and treatment knowledge and the frequency and proportion of iodine-rich foods consumption among pregnant women in iodine deficiency areas in Anhui Province have increased significantly. It is recommended to carry out publicity and education on IDD prevention and treatment knowledge in early pregnancy.

3.
Article in Chinese | WPRIM | ID: wpr-906762

ABSTRACT

@#Polydopamine (PDA) nanoparticles were prepared as a carrier, and bavachinin (BVA) was efficiently loaded by physical adsorption.The erythrocyte membrane was further utilized to modify and construct the erythrocyte membrane biomimetic nanoparticles (RBC-BP), the residence time in the body was extended and the in vivo analytical method was established to investigate their pharmacokinetics in mice.Polydopamine nanoparticles loaded with BVA (BP) were prepared by solvent replacement method, and the influencing factors of PDA loaded with BVA were investigated with the adsorption rate as the evaluation index.The erythrocyte membrane was extracted and separated, and RBC-BP was prepared by incubation coextrusion method. The effects of pH value on membrane coating and the extrusion times on the particle size and uniformity of RBC-BP were investigated.The particle size, potential, morphology, and cumulative release rate of RBC-BP were systematically characterized, and their pharmacokinetics in mice were preliminarily explored.The results showed that the adsorption rate of BP was as high as (92.08 ± 0.17) % and the drug loading rate was (42.05 ± 2.95) % at the PDA to BVA ratio of 1∶0.5, the solution pH value of 7, the incubation time of 6 h, and the incubation temperature of 20 °C, and that the erythrocyte membrane could be successfully oriented and coated on the surface of BP by the action of electric charge at the pH value of 4. The in vitro studies showed that RBC-BP has the apparent core-shell structure with the particle size of (308.63 ± 6.56) nm and good stability, and in vivo pharmacokinetic studies showed that RBC-BP can significantly extend the circulation time of nanoparticles in vivo.

4.
China Pharmacy ; (12): 2353-2357, 2021.
Article in Chinese | WPRIM | ID: wpr-886916

ABSTRACT

OBJECTIVE:To establish the method for the content de termination of 17 quality markers in Dahuang zhechong pills(DHZCP). METHODS :HPLC method was adopted to determine the contents of 17 quality markers in 10 batches of DHZCP , such as allantoin ,hypoxanthine,salidroside,hydroxypaeoniflorin,glycyrrhizin,isoglycyrrhizin,baicalin,p-methoxyphenylacetic acid,wogonin,cinnamic acid ,apigenin,naringin,norwogonin,aloe emodin ,rhein,chrysin,emodin. The determination was performed on Kromasil 100-5-C18(250 mm × 4.6 mm,5 μm)column with mobile phase consisted of 0.1% phosphoric acid solution-acetonitrile (gradient elution ) at the flow rate of 1.0 mL/min. The column temperature was 30 ℃ ,the detection wavelength was 210 nm and the sample size was 20 μ L. RESULTS:The linear range of above 17 quality markers were 5.74-183.53,6.51-208.24,4.30-137.65,4.60-147.06,4.12-131.76,4.25-135.88,6.31-201.76,4.60-147.06,1.94-62.06,4.47- 142.94,0.69-22.06,2.29-73.24,2.33-74.41,1.42-45.29,6.65-212.94,1.11-35.44 and 1.47-47.06 μg/mL,respectively(all R2≥ 0.999 0). RSDs of precision ,repeatability,stability and durability tests were all less than 2%(n=6);average recovery of 17 quality markers ranged from 96.31% to 101.73%,and the RSDs were less than 3%(n=6). CONCLUSIONS :The method is simple, rapid,speific,specise,reproducible,stable,accurate and durable ,and can be used for improving the quality standard of DHZCP.

5.
Article in Chinese | WPRIM | ID: wpr-883525

ABSTRACT

Objective:To observe the protective effects of Clostridium butyricum and butyrate on pancreas, liver and intestinal mucosa in rats with acute necrotizing pancreatitis (ANP), and to explore the possible mechanism.Methods:Forty Sprage-dawley rats were randomly divided into control group, ANP group,Clostridium butyricum treated group(CB group) and butyrate treated group(SB group), with 10 rats in each group by random number method. The ANP rat models were prepared by retrograde injection of sodium taurocholate into the biliopancreatic duct. Rats in CB and SB group were given intragastic administration of Clostridium butyricum 1×10 9 CFU or sodium butyrate (100 mg/kg) in 10 days once a day before modeling. Serum amylase (SAMY), lypase, ALT, AST, TBil, tumor necrosis factor alpha(TNF-α), IL-6 and HMGB1were measured after 24 h. Protein from intestinal mucosa was extracted and Western Blotting was used to measure expression of tight-junction proteins ZO-1, claudin-1 and occludin. Pancreas and liver tissues were stained with hematoxylin-eosin and scored by pathology. Results:The levels of amylase [(9365.1±716.5), (5947.3±512.0), (6517.7±269.6)U/L], lipase[(8343.7±1041.4), (6600.4±899.7), (6754.4±1046.4)U/L], AST[(560.5±72.7), (432.0±76.2), (429.8±40.5)U/L], ALT[(499.9±65.2), (385.7±46.0), (395.8±45.8)U/L], TBil[(134.2±56.2), (74.3±65.2), (81.3±35.3)U/L], TNF-α[(162.0±14.4), (100.4±6.3), (119.2±12.5)ng/L], IL-6[(161.4±26.0), (104.8±15.2), (105.5±12.7)ng/L], HMGB1[(100.1±6.7), (58.0±7.7), (63.4±7.2)ng/L] in ANP group, CB group and SB group were detected; and the pathological scores of pancreas[(11.2±1.08),(9.45±1.06), (9.04±0.89)] and liver[(2.89±0.73), (2.09±0.49), (2.12±0.52)] in ANP group,CB group and SB group were higher than those in control group[(100.6±5.20)U/L, (966.5±301.9)U/L,(30.2±6.3)U/L, (27.6±5.9)U/L, (2.4±0.6)U/L, (29.5±4.8)ng/L,(36.9±7.6)ng/L,(35.5±5.7)ng/L,(1.18±0.05),(0.56±0.09)]. However, those indexes in CB group and SB group were lower than those in ANP group, and the difference was statistically significant (all P<0.05). The expression of ZO-1 in control group, ANP group, CB group and SB group was 1.83, 0.79, 1.25 and 1.16. The expression of claudin-1 in control group, ANP group, CB group and SB group was 0.58, 0.13, 0.43 and 0.37. The expression of occludin in control group, ANP group, CB group and SB group was 1.06, 0.38, 0.82 and 0.79. The expression of TJ proteins in ANP group was significantly lower than that in other groups and the difference was statistically significant (all P<0.05). Conclusions:Clostridium butyricum and metabolites butyrate can alleviate the inflammatory response in ANP rats with liver injury, maintain the function of intestinal mucosal barrier and prevent the liver injury.

6.
Neuroscience Bulletin ; (6): 1325-1338, 2021.
Article in English | WPRIM | ID: wpr-922632

ABSTRACT

A strong animal survival instinct is to approach objects and situations that are of benefit and to avoid risk. In humans, a large proportion of mental disorders are accompanied by impairments in risk avoidance. One of the most important genes involved in mental disorders is disrupted-in-schizophrenia-1 (DISC1), and animal models in which this gene has some level of dysfunction show emotion-related impairments. However, it is not known whether DISC1 mouse models have an impairment in avoiding potential risks. In the present study, we used DISC1-N terminal truncation (DISC1-N


Subject(s)
Animals , Interneurons/metabolism , Mice , Mice, Transgenic , Nerve Tissue Proteins/metabolism , Neurons/metabolism , Nucleus Accumbens/metabolism , Parvalbumins/metabolism
7.
Chinese Journal of Endemiology ; (12): 764-768, 2020.
Article in Chinese | WPRIM | ID: wpr-866200

ABSTRACT

Objective:To establish a direct and rapid method for direct determination of arsenic in urine by inductively coupled plasma mass spectrometry (ICP-MS).Methods:The newly collected urine samples were diluted directly with pure water without pretreatment. The total arsenic content in urine was determined directly by ICP-MS. The method was studied from the aspects of linear range of standard curve, correlation coefficient, detection limit, precision and accuracy.Results:The concentration of arsenic in urine was in the range of 0 - 200 μg/L, the ratio of arsenic to germanium was in good linear relationship with arsenic concentration, the correlation coefficient was 0.999 5 - 0.999 9 ( n = 6), the lowest qualitative and quantitative detection limits of arsenic in urine were 0.66 and 1.94 μg/L(the sampling volume was 0.50 ml), respectively. Five urine samples with different arsenic concentrations were tested for intrabatch and inter batch precision with RSD ranging from 1.51% to 6.84% and 1.85% to 5.03%, respectively. The total arsenic of urine samples from the Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention was determined with this method, and the results were within the range of the published consensus value. The recovery rate of 4 urine samples with arsenic concentration range 3.19 - 89.36 μg/L was 99.25% - 103.67%, and the total average recovery rate was 101.51%. Conclusions:Application of ICP-MS method to detect arsenic content in urine, urine samples can be directly injected to realize the automation of injection, detection process and result analysis. The test parameters such as sensitivity, precision and accuracy of the method meet the requirements of the development of biological sample detection method and are suitable for rapid and direct determination of total arsenic in urine.

8.
Chinese Journal of Endemiology ; (12): 215-220, 2020.
Article in Chinese | WPRIM | ID: wpr-866096

ABSTRACT

Objective:To develop a standard method for determination of iodine in serum by inductively coupled plasma mass spectrometry (ICP-MS).Methods:A direct dilution sampling-ICP-MS method for measuring iodine in serum was developed with 2.0 g/L ascorbic acid-1.0 g/L ammonium chloride-0.10% ethanolamine-1.0% ethanol as diluent; the standard solutions and the serum samples were all diluted in a ratio of 1 : 19 (sample : diluent) before testing with Re as internal standard element. Methodological evaluation of a new method was done through standard curve linearity, sample detection limit, precision and accuracy in determining serum iodine. And the results were compared with the results determined by current standard method for determination of iodine in serum [arsenic-cerium catalytic spectrophotometry, hereinafter referred to as the standard method (WS/T 572-2017)].Results:The following data were obtained by testing the method in six laboratories. The linear range of the calibration curve was 0 - 300 μg/L and the linear correlative coefficients were 0.999 9 - 1.000 0 ( n = 70). The detection limit for serum iodine was 0.2 to 1.3 μg/L (0.20 ml of serum was tested). Precision: the average intra assay coefficient of variation ( CV) was 0.6% with a range of 0.2% - 1.6% when measuring 31 serum samples; the average inter assay CV was 1.3% with a range of 0.3% - 2.4% when measuring 34 serum samples. Accuracy: the average recovery was 100.3% with a range of 93.9% - 105.6% when measuring 36 serum samples. No significant difference was found between the results of the 116 serum samples determined by the new method and the standard method ( P > 0.05). Conclusions:The new method has a good standard curve linearity, high sensitivity, good precision, accuracy and is easy to be used and quickly to be analyzed of the test results, and is suitable for widely application in determining serum iodine.

9.
Chinese Journal of Endemiology ; (12): 221-224, 2020.
Article in Chinese | WPRIM | ID: wpr-866093

ABSTRACT

Objective:To establish an enzyme-labeled instrument automatic colorimetric method for determination of iodine content in human urine.Methods:The enzyme-labeled instrument automatic colorimetric method was used to determine the iodine content in human urine. The linear range, detection limit and precision of the method were verified. And the urine iodine test results were compared with the results tested by national health industry standard as arsenic-cerium catalytic spectrophotometry.Results:The linear range of iodine standard curve of the enzyme-labeled instrument automatic colorimetric method was 0 - 300 μg/L, the linear correlation coefficient ( r) was - 0.999 5 to - 0.999 2, and the detection limit was 6.5 μg/L. The relative standard deviation ( RSD) of urine samples with low, medium and high iodine concentration were all < 3%, the recovery rate ranged from 92.2% to 109.2%, and the total average recovery rate was 99.6%. There was no significant difference in the detection results of iodine content in 40 urine samples between the enzyme-labeled instrument automatic colorimetric method and arsenic-cerium catalytic spectrophotometry ( t = 1.347, P > 0.05); and the detection speed of the enzyme-labeled instrument automatic colorimetric method was 7.5 times of the arsenic-cerium catalytic spectrophotometry. Conclusion:The enzyme-labeled instrument automatic colorimetric method has a reasonable linear range, good precision and high accuracy in determination of urinary iodine content, and the enzyme-labeled instrument automatied fast colorimetry has improved the analysis speed, it is suitable for detection of large quantities of samples.

10.
Article in Chinese | WPRIM | ID: wpr-866065

ABSTRACT

Objective:To establish an automatic colorimetric method for determination of iodine in drinking water by enzyme-labeled instrument (hereinafter referred to as this method).Methods:The water iodine was measured in the range of 0 - 10 μg/L and 0 - 100 μg/L, experiments were carried out on linear relationship, detection limit, precision and accuracy of this method. And the results were compared with the National Reference Laboratory for Iodine Deficiency Disorders recommended arsenic cerium catalytic spectrophotometry method.Results:In the range of 0 - 10 μg/L and 0 - 100 μg/L, all│ r│ > 0.999 0, the detection limits were 0.6 and 1.1 μg/L (samples were 200 and 100 μl), respectively; the relative standard deviation ( RSD) of water samples of low, medium and high iodine mass concentrations were < 3%, the recovery rates ranged from 92.5% to 108.3% and 93.2% to 108.9%, with a total average recovery of 100.0% and 100.3%, respectively. This method and arsenic cerium catalytic spectrophotometry method were used to detect 40 water samples in the range of 0 - 10 μg/L and 0 - 100 μg/L, there was no significant difference in water iodine content between the two methods ( t = 0.99, P > 0.05). Conclusion:This method has good linear curve relationship for determination of water iodine content, good precision and high accuracy, and it is suitable for wide application.

11.
Article in Chinese | WPRIM | ID: wpr-871050

ABSTRACT

We report a critically ill pregnant woman in the third trimester with severe pneumonia due to COVID-19 who presented to Xiaolan People's Hospital of Zhongshan in February 2020. The 32-year-old patient was admitted at 35 +2 gestational weeks with a 4-day history of a sore throat and a fever for three hours. The patient had been to Xiaogan City, Hubei Province, and the symptoms occurred during a period of self-isolation after back home. The condition of the patient deteriorated rapidly, with left-sided chest and back pain, shortness of breath, dizziness, progressing to respiratory failure and septic shock 7 hours after her admission. In view of her critical condition and a history of two previous cesarean sections, an emergency cesarean section was performed. Blood gas analysis of the mother before the operation suggested respiratory failure, respiratory acidosis, and metabolic acidosis. During the operation, a baby boy was born. The Apgar score of the boy, birth weight of 2 700 g, was one at 1, 5, and 10 minutes despite the resuscitation efforts. The neonate died after withdrawing treatment. The patient was treated with tracheal intubation ventilator and other supportive treatments after the operation. The result of the new coronavirus nucleic acid test, taken on admission, but which was reported after delivery, was positive. The patient was transferred to the designated hospital for further treatment and was recovering with the withdrawal of extracorporeal membrane oxygenation and ventilation at 26 and 36 days after surgery, respectively.

12.
Article in Chinese | WPRIM | ID: wpr-819274

ABSTRACT

We report a critically ill pregnant woman in the third trimester with severe pneumonia due to COVID-19 who presented to Xiaolan People's Hospital of Zhongshan in February 2020. The 32-year-old patient was admitted at 35 +2 gestational weeks with a 4-day history of a sore throat and a fever for three hours. The patient had been to Xiaogan City, Hubei Province, and the symptoms occurred during a period of self-isolation after back home. The condition of the patient deteriorated rapidly, with left-sided chest and back pain, shortness of breath, dizziness, progressing to respiratory failure and septic shock 7 hours after her admission. In view of her critical condition and a history of two previous cesarean sections, an emergency cesarean section was performed. Blood gas analysis of the mother before the operation suggested respiratory failure, respiratory acidosis, and metabolic acidosis. During the operation, a baby boy was born. The Apgar score of the boy, birth weight of 2 700 g, was one at 1, 5 and 10 minutes despite the resuscitation efforts. The neonate died after withdrawing treatment. The patient was treated with tracheal intubation ventilator and other supportive treatments after the operation. The result of the new coronavirus nucleic acid test, taken on admission, but which was reported after delivery, was positive. The patient was transferred to the designated hospital for further treatment and was recovering with the withdrawal of extracorporeal membrane oxygenation and ventilation support at 26 and 36 days after surgery, respectively.

13.
Article in Chinese | WPRIM | ID: wpr-804835

ABSTRACT

Objective@#To construct influenza B virus Vero cell adapted strain by genetic recombination technology by using the influenza B virus Vero cell adapted strain as the parent strain.@*Methods@#The chick embryo and Vero cell were co-infected with influenza virus Vero cell adapted strain B/Malaysia/2506/2004 Va (Bv) and the vaccine strain B/massachusetts/2/2012 (BX-51B) recommended by WHO. The reassortants were screened with the anti-Bv serum. Plaque-purified reassortants were used to screen for Vero cell-adapted influenza B virus strains containing the surface antigen of the epidemic strain.@*Results@#A Vero cell-adapted influenza B virus strain was obtained with successive passage in Vero cells. The hemagglutination inhibition test and the one-way immunogold agar diffusion test both showed that the reassortant virus was homologous to NYMC BX-51B, and sequence analysis result showed that the reassortment virus has the same HA and NA gene with the vaccine strain.@*Conclusion@#B/Malaysia/2506/2004Va (Bv) can be used as a parent strain to prepare Vero cell vaccine against influenza B virus.

14.
Article in Chinese | WPRIM | ID: wpr-754461

ABSTRACT

Objective: To investigate the prognosis of patients who receive neoadjuvant chemotherapy (NAC) for invasive micropapillary carcinoma (IMPC) of the breast using a propensity score matching (PSM) method and to analyze the effects of NAC. Methods: Clinical and pathological data of a total of 251 cases of IMPC of the breast were collected for this study, from January 2011 to March 2014 in Tianjin Medical University Cancer Institute and Hospital, of which the NAC group comprised 67 cases and the non-NAC group comprised 184 cases. Tumor sizes before and after NAC were compared in the NAC group. Prognostic differences were compared between the NAC group and non-NAC group before and after PSM balancing the baseline. Results: The mean value of the maximum dimensions significantly reduced from 5.0cm to 4.2cm in the NAC group after NAC (P=0.035), but there was no statistically significant difference in T stage changes (P=0.064). A total of 49 pairs of patients were matched after PSM, and differences in the baseline data of the paired group were not significant. Univariate survival analysis showed no significant difference in the recurrence-free survival (RFS) rate between the NAC group (77.6% vs. 89.2%) and non-NAC group (72.1% vs. 91.0%) before and after PSM (all P>0.05). The 5-year distant metastasis-free survival (DMFS) rates in the NAC group before and after PSM were 53.4% and 50.0%, respectively, which were both significantly lower than those in the non-NAC group 69.1%, 59.2% (all P<0.05), and multivariate survival analysis showed that undergoing NAC was an independent prognostic factor of DMFS after PSM. Conclusion: Breast IMPC is a special type of tumor that is not sensitive to chemotherapy. Although some tumors decrease after NAC, IMPC patients do not benefit from NAC in terms of RFS; NAC may even increase the risk of distant metastasis. Therefore, IMPC patients should undergo surgical treatment as soon as possible, and NAC is not recommended.

15.
Article in Chinese | WPRIM | ID: wpr-754391

ABSTRACT

With the development and application of microarray and high-throughput sequencing technology, it is possible to genomi-cally scan breast cancer associated CNV and SNPs. Multiple researches showed that the frequent aberrance of 8q24 was associated with breast cancer. Oncogenes such as MYC, PSCA and breast cancer associated loci are located in 8q24, and frequent amplification of 8q24 was proposed to be related with breast cancer development and prognosis. This review summarizes the association of the 8q24 amplification and SNPs with breast cancer by correlated genes on this locus.

16.
Article in Chinese | WPRIM | ID: wpr-753377

ABSTRACT

Objective To explore the effect of Clostridium butyricum ( C. butyricum ) and its metabolite butyrate on the function of intestinal mucosal barrier and intestinal flora in acute necrotizing pancreatitis ( ANP) rats with intra-abdominal hypertension ( IAH) . Methods Eighty SD rats were randomly divided into normal control group (A group, n=20), ANP with IAH group(B group, n=20), ANP with IAH and C. butyricum treated group ( C group, n=20 ) , ANP with IAH and sodium butyrate treated group ( D group, n=20). Rats of C and D group were given intragastric administration of C. butyricum 1 × 109 CFU once a day or 100 mg/kg sodium butyrate once a day from 10 days before modeling. Sodium taurocholate injection method via pancreatobiliary ducts was used to establish ANP with IAH rat model, and the intra-abdominal pressure was measured by direct puncture of left lower belly 24 h after modeling. Blood samples were collected for detecting serum amylase(AMY), tumor necrosis factor alpha (TNF-α), diamine oxidase( DAO ) , lipopolysaccharide ( LPS ) and D-Lactate, and the pathological changes of terminal ileum was observed. Real-time quantitative PCR was used to detect the populations of 6 bacteria in ileum mucosa. Results The levels of AMY, TNF-α, LPS,DAO, D-Lactate and ileum mucosa score were obviously higher in B, C and D group than those in A group, but the number of piobiotic flora in ileum mucosa was lower than that in A group, while the number of pathogenic bacteria was higher than that in A group. The levels of LPS, DAO, D-Lactate and ileum mucosa pathological score were lower in C group and D group than those in B group, but the number of piobiotic flora in ileum mucosa was lower than that in B group, while the number of pathogenic bacteria was higher than that in B group. All the differences above were statistically different (P<0.05). Conclusions C. butyricum and butyrate can maintain the function of intestinal mucosal barrier in ANP rats with IAH, and also readjust the imbalance of intestinal flora.

17.
Article in Chinese | WPRIM | ID: wpr-745161

ABSTRACT

Objective To evaluate the left ventricular diastolic function of patients with normal left ventricular ejection fraction ( LVEF) by echocardiography and real‐time cardiac catheter measurement ,and improve the accuracy and reliability of echocardiographic diagnosis . Methods One hundred and twenty patients with know n or suspected coronary artery disease w ho underwent coronary angiography and left ventricular catheterization were prospectively selected from July 2017 to January 2018 in the Affiliated Hospital of Jiangsu University . According to the left ventricular end diastolic pressure ( LVEDP) real‐time measurement ,the patients were divided into groups of LVEDP ≤15 mm Hg ( 43 cases ) and LVEDP > 15 mm Hg ( 77 cases) . General data were compared and the difference of echocardiographic parameters between the two groups were analyzed ,and the ROC curve of each echocardiographic parameter for diagnosing LVEDP was draw n . Results T he parameters including flow propagation velocity ( VP) ,the ratio of filling fraction of E and A ( E/A) ,early diastolic filling deceleration time ( DT ) ,the duration of mitral A ( A‐dur ,) mitral annulus velocity at the septal side ( e′sep) ,systolic pulmonary venous flow velocity ( PVs) ,diastolic pulmonary venous flow velocity ( PVd ) and PVs/PVd were used to the diagnosis of the increasing of LVEDP ,however their accuracies were low ( AUC between 0 .5~0 .7) . T he parameters including left atrial volume index ( LAVI ) , tricuspid regurgitation ( T Rmax ) ,mitral annulus velocity in lateral wall of left ventricle ( e′lat ) ,average e′,E/e′sep ,E/e′lat ,average E/e′,velocity of pulmonary vein atrial reversal ( PVa) ,pulmonary vein atrial reversal duration ( Pva‐dur) ,the difference between the duration of pulmonary venous A wave and mitral A wave( PvaD‐AD) were also used to the diagnosis of the increasing of LVEDP , but their accuracies were still poor ( AUC between 0 .7~0 .9 ) . According to the real‐time left ventricular pressure measurement and different parameters of echocardiography ,the multivariate regression equation :LVEDP= 0 .292 LAVI + 0 .35 PVa + 0 .04 T Rmax + 0 .075 ( PvaD‐AD ) -0 .109 PVs -6 .773 was put forward as a correction standard ,the accuracy of the diagnosis of LVEDP was significantly improved ( AUC =0 .922) . Conclusions T he assessment of left ventricular diastolic function needs to be performed comprehensively with multiple parameters . T he multiple regression equation can accurately evaluate left ventricular diastolic function in patients with normal LVEF .

18.
Chinese Journal of Endemiology ; (12): 325-329, 2019.
Article in Chinese | WPRIM | ID: wpr-744308

ABSTRACT

Objective To determine serum iodine by inductively coupled plasma mass spectrometry (ICP-MS).Methods ICP-MS was used to determine iodine content directly by pretreatment method of diluting serum samples and standard series with diluent.The method was tested from correlation coefficient of standard curve,detection limit,precision,accuracy,etc.Results When the concentration of iodine in serum ranged from 0 to 300 μg/L,the linear correlation coefficient with the iodine ion counts determined by the instrument was 0.999 8 to 1.000 0 (n =6);the detection limit of iodine in serum was 1.24 μg/L;the relative standard deviation (RSD) ranged from 0.5% to 1.5% and from 0.3% to 1.4% for the intra-and inter-batch precision tests of seven different serum samples,respectively;the recovery rate of 7 serum samples with iodine content 40-230 μg/L ranged from 93.4% to 109.2%,and the total average recovery rate was 102.4%.Conclusions Serum samples need not be pretreated before digestion,and can be directly injected after dilution with diluent without using highly toxic substances.The detection process is automatic,sensitive,precise,accurate and suitable for rapid quantitative determination of serum iodine content.

19.
Journal of Breast Cancer ; : 237-247, 2019.
Article in English | WPRIM | ID: wpr-764266

ABSTRACT

PURPOSE: Breast cancer (BC) is one of the most common malignancies globally, and millions of women worldwide are diagnosed with BC every year. Up to 70% of BC patients are estrogen receptor (ER)-positive. Numerous studies have shown that tamoxifen has a significant therapeutic effect on both primary and metastatic ER-positive BC patients. Although tamoxifen is currently one of the most successful therapeutic agents for BC, a significant proportion of patients will eventually become resistant to tamoxifen, leading to tumor recurrence and metastasis. Knowledge about the development of tamoxifen resistance in BC patients is still limited. METHODS: We applied a loss-and-gain method to study the biological functional role of cell division cycle associated 8 (CDCA8) in tamoxifen resistance in BC cells. RESULTS: We found that CDCA8 was significantly elevated in tamoxifen-resistant BC cells. Knockdown of CDCA8 expression significantly inhibited the proliferation of tamoxifen-resistant BC cells and reduced their resistance to tamoxifen. In contrast, overexpression of CDCA8 promoted the growth of tamoxifen-sensitive BC cells and induced their resistance to tamoxifen. CONCLUSION: In this study, we reported that CDCA8 is a key regulator of tamoxifen resistance in BC, suggesting that CDCA8 may serve as a potential therapeutic target for BC treatment.


Subject(s)
Apoptosis , Breast Neoplasms , Breast , Cell Cycle , Cell Division , Estrogens , Female , Humans , Methods , Neoplasm Metastasis , Recurrence , Tamoxifen
20.
Chinese Journal of Endemiology ; (12): 747-750, 2019.
Article in Chinese | WPRIM | ID: wpr-790920

ABSTRACT

Objective To verify the feasibility and application value of arsenic-cerium catalytic spectrophotometry for determination of iodine in serum.Methods The blood samples of adults were collected and the iodine standard curve of 0-300 μg/L was prepared.Referring to "The Method for Determination of Chemical Substances in Biomaterials",the present method was tested in terms of linear relationship,minimum detection limit,precision and accuracy.Results The correlation coefficient of the method was-0.999 7--0.999 4 (n =6) and the minimum detection limit was 5.13 μg/L in the range of 0-300 μg/L standard curve.Precision verification displayed that the variable coefficient of three different iodine concentration serum samples were 0.89%,1.88%,0.67%.And the accuracy verification displayed that the recovery of iodine standard was 93.90%-107.04%,and the total average recovery was 99.48%.Conclusion The method has been proved to be of good linearity,high precision and high accuracy in the determination of serum iodine,which meets the requirements of biological sample analysis.

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