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1.
Article in English | WPRIM | ID: wpr-921344

ABSTRACT

The main purpose of the National Nutrition and Health Systematic Survey for children 0-17 years of age in China (CNHSC) was to collect basic data on the nutrition, development, and health status for children in different regions across China using evidence-based, reliable, and cost-effective approaches. Children and their parents or guardians from seven regions (south, southwest, north, northwest, eastern, central, and northeast China) in China were recruited. A multi-stage stratified randomized sampling method was used. Two provinces were randomly sampled from each of the seven regions, from which one urban district and one rural country were also randomly sampled, resulting in a total of 28 survey counties/districts. Dietary surveys, health examinations, laboratory testing, and questionnaires were used to collect dietary intake, nutritional status, child development, and health status information. Nutrition, health, and lifestyle assessment of children and their parents was determined using the Knowledge Attitude Practice (KAP) survey. Greater than 100,000 children (38,000 children < 6 years of age and 66,000 children 6-17 years of age) completed the survey. The survey provided comprehensive data on child nutrition and health status for future studies and will serve as the basis for an integrated nutrition and health improvement strategies proposal for children in China.


Subject(s)
Adolescent , Child , Child Development , Child, Preschool , China , Health Status , Health Surveys , Humans , Infant , Infant, Newborn , Nutrition Surveys
2.
Article in English | WPRIM | ID: wpr-915084

ABSTRACT

Objective@#This study aimed to determine the effect of radiotherapy (RT) on the risk of herpes zoster (HZ) in patients with gynecological cancers via a nationwide population-based study. @*Methods@#Based on patient data obtained from the National Health Insurance Research Database, 1928 gynecological cancer patients were identified with 1:1 matching for RT and non-RT cohorts by age, index date, and cancer type. Another cohort consisting of 964 noncancer individuals matched was used as normal control. The incidence of HZ was compared between cancer patients with and without RT. Age, comorbidities, cancer-related surgery and chemotherapy (CT), and cancer type were adjusted as confounders. @*Results@#The risk of HZ in cancer patients was higher than that of non-cancer individuals (14.23 versus 8.34 per 1,000 person-years [PY], the adjusted hazard ratio [aHR]=1.38, p=0.044). In the cancer population, the incidence of HZ for the RT and non-RT cohorts was 20.55 versus 10.23 per 1,000 PY, respectively (aHR=1.68, p=0.009). Age >50 years was an independent factor for developing HZ. The 5-year actuarial incidence for patients receiving neither RT nor CT, RT alone, CT alone, and combined modalities was 5.4%, 6.9%, 3.7%, and 9.9%, respectively (p<0.001). In the RT cohort, the risk rose rapidly in the first year, becoming steady thereafter. @*Conclusion@#This population-based study showed that gynecological cancer patients receiving RT combined with CT had the highest cumulative risk of HZ. Health care professionals should be aware of the potential toxicities.

3.
Article in Chinese | WPRIM | ID: wpr-912726

ABSTRACT

Objectives:To investigate the current research status and focus of multi-campus construction and management in public hospitals, and to provide research reference and policy recommendations.Methods:The CNKI and Wanfang databases were searched from the establishment of the databases to October 31, 2020. Literatures of multi-campus construction and management in public hospitals were screened following inclusion and exclusion criteria. Bibliometric analysis was used to analyze the characteristics of the included literatures.Results:A total of 99 articles were included. In 2019, there were 18 articles, which was the most over the years. The number of published literatures has increased significantly since 2014. The most common origin cities of publications were Shanghai, Wuhan and Beijing. Literature research focused on integration, homogeneity, economic operation, cultural construction and development strategy.Conclusions:The development of multi-campus in public hospitals has attracted more and more attention, but it has not yet involved in the research of setting access, functional positioning, construction mode, peacetime and wartime transformation, and it is predicted that it will become a research hotspot in the future.

4.
Chinese Journal of Geriatrics ; (12): 1025-1029, 2021.
Article in Chinese | WPRIM | ID: wpr-910960

ABSTRACT

Objective:To investigate the relationships between severe, diffuse decrease in global brain 18F-fluorodeoxxglucose(FDG)uptake, whole-body total lesion glycolysis(TLG)and short-term death in elderly patients with newly diagnosed stage Ⅳ cancer. Methods:Clinical and 18FFDG PET/CT data of 24 elderly patients newly diagnosed stage Ⅳ cancer showing marked diffuse decrease in global brain FDG uptake(the decreased brain uptake group)were retrospectively enrolled.Sixteen elderly patients with newly diagnosed stage Ⅳ cancer but without decreased global brain FDG uptake(the no decreased brain uptake group)and 25 healthy subjects were enrolled as the control groups.Correlations between brain FDG uptake and whole-body TLG were analyzed.We followed up the final outcomes of all patients and analyzed the short-term prognostic value of these manifestations. Results:The decreased brain uptake group included 17 patients with stage Ⅳ lymphoma and 7 patients with stage Ⅳ malignant tumor of other types[15 males, age: (73±9)years], while the no decreased brain uptake group included 8 patients with stage Ⅳ lymphoma and 8 patients with stage Ⅳ malignant tumor of other types[12 males, age: (65±5)years]and the healthy control group included 25 subjects[13 males, age: (65±6)years]. Patients were older in the decreased brain uptake group than in the no decreased brain uptake group( t=3.8, P=0.001). The global brain SUV means of the decreased brain uptake group and the no decreased brain uptake group were 4.9±1.8 and 10.9±2.0, respectively( t=-9.8, P=0.000). The global brain total glycolysis(TG)values of the two groups were 1786.5±1162.5 and 2868.4±1424.5, respectively( t=-2.6, P=0.012). The whole-body TLG values of the two groups were 6825.5±4776.9 and 2919.5±2031.7, respectively( t=3.6, P=0.001). Pearson correlation analysis showed that brain FDG uptake was adversely correlated with whole-body TLG.We followed up the survival outcomes of the two groups.The median follow-up lengths of the two groups were 6 months and 10 months, respectively( χ2=3.7, P=0.054). Fourteen(14/24)patients died in the decreased brain uptake group while 9(9/16)died in the no decreased brain uptake group( χ2=0.017, P=0.896). However, 8 cases died within 1 month post PET/CT scan in the decreased brain uptake group while none died in the no decreased brain uptake group( χ2=4.7, P=0.029). Conclusions:Severe, diffuse decrease in 18F-FDG PET/CT uptake in the whole cerebral cortex is more common in elderly patients with newly diagnosed stage Ⅳ cancer, whose total tumor load is significantly higher than that of cancer patients without decrease in whole cerebral cortex FDG uptake.This uptake reduction may indicate poor short-term outcome and the probability of short-term death may be high.

5.
Chinese Journal of Radiology ; (12): 1036-1041, 2021.
Article in Chinese | WPRIM | ID: wpr-910265

ABSTRACT

Objective:To explore the value of blood-brain barrier permeability (BBBP) parameters based on CT perfusion (CTP) in predicting delayed cerebral ischemia (DCI) in patients with aneurysmal subarachnoid hemorrhage (aSAH) within 24 hours of admission.Methods:Totally 69 patients underwent whole-brain CTP within 24 h after aneurysm rupture from July to November 2020 in Yijishan Hospital of Wannan Medical College. The volume transfer constant (K trans) reflecting BBBP, the time to drain (TTD) and transit time to the center of the impulse response function (TMax) reflecting cerebral perfusion were obtained. Patients were divided into DCI and non-DCI groups. Quantitative and qualitative CTP parameters, clinical data were compared between the two groups. On the basis of univariate analysis, the multivariate logistic regression analysis was used to determine the independent risk factors of DCI using the stepwise regression method. The predictive efficiency of clinical data and CTP parameters were evaluated by ROC analysis. Results:Twenty-one of 69 aSAH patients developed DCI. Whole brain average values of K trans (mK trans) in the DCI group [(0.67±0.16)ml/(100 ml·min)] were significantly higher than those in the non-DCI group [(0.41±0.15)ml/(100 ml·min), t=-6.454, P<0.001]. mK trans in the diffused hypoperfusion patients [(0.61±0.18)ml/(100 ml·min)] was significantly higher than that in the normal perfusion group [(0.36±0.15)ml/(100 ml·min), P<0.001] and localized hypoperfusion group [(0.43±0.16)ml/(100 ml·min), P<0.001]. Multivariate logistic regression analysis showed mK trans (OR=1.13, 95%CI 1.05-1.21, P=0.001), World Federation of Neurosurgery Scale (OR=5.35, 95%CI 1.12-25.65, P=0.036) and modified Fisher Score (OR=5.32, 95%CI 1.02-27.80, P=0.048) were significantly independent predictors of DCI. ROC curve analysis revealed that mK trans produced the highest AUC of 0.875 (95%CI 0.78-0.97), with a threshold of 0.545 ml/(100 ml·min) had sensitivity of 85.7%, specificity of 79.2% and Youden index of 64.9% for prediction of developing DCI. Conclusion:It is feasible to evaluate cerebral perfusion and BBBP status and predict the risk of developing DCI in aASH patients who admitted within 24 h after aneurysm rupture using whole-brain CTP.

6.
Article in Chinese | WPRIM | ID: wpr-909537

ABSTRACT

Objective:To investigate the effect of intranasal oxytocin administration on empathy in male adolescents with conduct disorder.Methods:The male adolescents with conduct disorder in the Psychological Counseling Clinic of the Second Xiangya Hospital of Central South University from September 2015 to August 2016 were selected.And they were randomly assigned to oxytocin group ( n=46) and placebo group ( n=51) by random number table. Subjects in oxytocin group were given nasal spray of 24 IU oxytocin twice per day for two weeks, while those in placebo group were given nasal spray of 0.9% sodium chloride solution for two weeks. The empathy of patients was assessed with a pain-related empathy task and interpersonal reactivity index (IRI) before and after two weeks′ administration. SPSS 20.0 software was used for statistical analysis, and repeated measurement analysis of variance was used to compare the empathy ability of the two groups before and after intervention. Results:Repeated measurement analysis of variance showed that there were significant time×group interaction effects in the scores of painful expressions during the pain-related empathy task ( F=13.86, P<0.001), IRI ( F=5.59, P=0.020) and empathic concern subscale ( F=4.99, P=0.028). There was significant between-group effect in the score of perspective-taking subscale of IRI( F=4.22, P=0.043). Simple effect analysis revealed that after two weeks of intervention, the score of needle-pain expression in oxytocin group was significantly higher than that at baseline ( t=-2.08, P=0.040). And the score of needle-pain expression in oxytocin group was significantly higher than that in placebo group ( t=2.33, P=0.022). After two weeks of intervention, the total IRI score ( t=-2.58, P=0.011) and empathy factor score ( t=-3.15, P=0.002) of oxytocin group were both higher than those at baseline. After intervention, the total IRI score ( t=2.30, P=0.024) and perspective-taking factor score ( t=2.57, P=0.012) in oxytocin group were higher than those in placebo group, and the differences were statistically significant. Conclusions:Oxytocin may improve the cognitive and emotional empathy in male adolescents with conduct disorder.

7.
Article in Chinese | WPRIM | ID: wpr-906208

ABSTRACT

Objective:To explore the anti-hepatoma effect of compound <italic>Phylanthus urinaria</italic> Ⅱ ( CPU Ⅱ) by inhibiting the expression of the long non-coding RNA (lncRNA) colon cancer associated transcript-1 (CCAT1) and restoring the expression of microRNA let-7a. Method:Real-time fluorescence quantitative polymerase chain reaction (PCR) was used to detect the expression of lncRNA CCAT1 in normal liver cells (LO2 cells) and hepatocellular carcinoma HepG2 cells, and the differences in expression between these two types of cells were compared. The methylthiazolyl tetrazolium(MTT) assay was used to detect the proliferation of HepG2 cells after treatment with different concentrations of CPU Ⅱ and 5-fluorouracil(5-FU) for 24, 48 and 72 h. Hepatocellular carcinoma HepG2 cells were cultured <italic>in vitro </italic>and set into three gropes: cell control group, CPU Ⅱ low-dose group (0.8 g·L<sup>-1</sup>) and high-dose group (1.6 g·L<sup>-1</sup>). Real-time PCR was used to detect the mRNA expression of lncRNA CCAT1, microRNA let-7a and its target genes high mobility group protein A2(HMGA2), and N-RAS in each grope. Western blot was used to detect the protein expression of HMGA2, and Cyclin D<sub>1</sub> in each grope. Result:As compared with LO2 cells, expression of lncRNA CCAT1 in HepG2 cells was significantly up-regulated (<italic>P</italic><0.05). Results of MTT assay showed that the 50% inhibiting concentration(IC<sub>50</sub>)<sub> </sub>of CPU Ⅱ and 5-FU on hepatocellular carcinoma HepG2 cells was 1.649, 0.044 648 g·L<sup>-1 </sup>respectively. As compared with the control group, CPU Ⅱ high-and low-dose groups (1.6, 0.8 g·L<sup>-1</sup>) significantly inhibited the proliferation of HepG2 cells (<italic>P</italic><0.05), and the effect was most remarkable in CPU Ⅱ high-dose group (<italic>P</italic><0.05). The results of Real-time PCR showed that as compared with control group, the expression of lncRNA CCAT1 mRNA was significantly inhibited in CPU Ⅱ high-and low-dose groups (<italic>P</italic><0.05), and the expression of microRNA let-7a mRNA was obviously up-regulated in high-dose group (<italic>P</italic><0.05), but the expression of HMGA2 mRNA in CPU Ⅱ high-and low-dose groups as well as the expression of N-RAS mRNA in CPU Ⅱ low-dose group were down-regulated (<italic>P</italic><0.05). Western blot results showed that as compared with the cell control group, the protein expression of HMGA2 and Cyclin D<sub>1</sub> in CPU Ⅱ high-and low-dose groups (1.6, 0.8 g·L<sup>-1</sup>) was significantly down-regulated (<italic>P</italic><0.05). Conclusion:CPU Ⅱ can inhibit the expression of lncRNA CCAT1, recover the expression of microRNA let-7a, and suppress the mRNA and protein expression of related downstream target genes in hepatoma cells line HepG2, thereby inhibiting the proliferation of hepatocellular carcinoma cells and exerting anti-hepatocellular carcinoma effect.

8.
Article in Chinese | WPRIM | ID: wpr-905818

ABSTRACT

Objective:This study was to explore the effect of HBM-based education and to help establish the measures on improving oral health status in deaf-dumb students. Methods:A total of 152 students aged 9-18 from two deaf-mute schools in Jing'an District, Shanghai were selected. The study subjects were randomly assigned to either experimental or control group. Students in the control group received oral health education with sign language every 6 months, and students in the experimental group received oral health education based on HBM. Before and two years after the intervention, data were collected by oral examinations and questionnaires. SPSS 22.0 software package was used for statistical processing of the data. Results:Before the intervention, there was no significant difference between the control group and the experimental group in caries rate, soft scale detection rate, calculus detection rate, caries average, debris index and calculus index. After the intervention, the caries rate (47.3% vs 68.5%), soft scale detection rate (77.0% vs 91.8%), and calculus detection rate (37.8% vs 58.9%) in the experimental group were all significantly (P<0.05) lower than those in the control group. After the intervention, caries average, debris index and calculus index in the experimental group were 1.93±2.25, 0.55±0.45, and 0.37±0.50, respectively, while the corresponding values in the control group were 2.82±3.24, 1.17±0.47, and 0.41±0.44. The difference in debris index between two groups was statistically significant (P<0.05). Conclusion:Oral health education for deaf-mute students based on Health Belief Model(HBM)shows a good effect on improving the oral health. Further oral health education as well as the implementation of intervention measures such as using local fluoride, pit and fissure sealing, and early dental caries filling, are needed to effectively control the occurrence and development of dental caries in deaf-mute students.

9.
Article in Chinese | WPRIM | ID: wpr-905081

ABSTRACT

Objective:To systematically evaluate the efficacy and safety of modified Buyang Huanwu Tang in the treatment of chronic heart failure. Method:CNKI database,Wanfang database,VIP database,Pubmed,MEDLINE,EMBASE and Cochrane database were retrieved systematically. The literature retrieval period is from no limit to December 2019,with "Buyang Huanwu Tang" and "chronic heart failure" "heart failure" as the key words for full-text retrieval of Chinese and English databases. Literatures of randomized controlled trials(RCTs) for chronic heart failure were included, and the data were extracted. Cochrane system evaluation method was used to score the quality of literature. Stata 14.0 was applied in Meta-analysis on the retrieval results. TSA0.9 was applied in test sequential analysis. Sensitivity analysis was made to explain heterogeneity,and funnel chart was used to evaluate publication bias. Result:A total of 2 037 patients were included in 21 RCT studies. The article quality risk assessment was generally unclear risk of bias. The results of meta-analysis showed that the left ventricular ejection fraction (LVEF) in the experimental group was significantly higher than that in the control group,with statistically significant differences [MD=0.901,95% CI (0.772,1.029),P<0.01],the left ventricular end diastolic diameter (LVEDd) in the experimental group was significantly lower than that in the control group,with statistically significant differences [OR=-0.650,95% CI=(-0.854,-0.446),P<0.01],BNP in the experimental group was significantly lower than that in the control group,with statistically significant differences [MD=-1.212,95% CI=(-1.359,-1.066),P<0.01],6-minute walk test (6MWT) in the experimental group was significantly higher than that in the control group,with statistically significant differences [MD=0.797, 95% CI=(0.447,1.146),P<0.01],and the effective rate in the experimental group was significantly improved,with statistically significant differences [OR=1.840,95% CI=(1.680,2.016),P<0.01]. Conclusion:Modified Buyang Huanwu Tang combined with conventional western medicine treatment of chronic heart failure is more effective than single administration of western medicine treatment,and can improve clinical efficacy, effectively improve the LVEF of patients with chronic heart failure,reduce the LVEDd reduces plasma BNP levels,prolong the 6-minute walking distance,and reduce the incidence of adverse reactions.

10.
Article in Chinese | WPRIM | ID: wpr-876159

ABSTRACT

Objective:To summarize the international experience in colorectal cancer population screening programs, so as to provide reference for the development and promotion of similar programs in China. Methods:We used “colorectal cancer population screening program” as key words to search the database of CNKI and Web of Science in this study. Results:A total of 18 reports (including 17 programs) were selected from 187 Chinese and 1 256 English literatures. International colorectal cancer screening programs were organized to target asymptomatic 50-74 years old population for fecal immunochemical test every two years and recommend participants with positive result to take further colonoscopy. These programs reduced the incidence of colorectal cancer and adenoma, which were beneficial to early diagnosis and treatment in colorectal cancer patients. They also showed good cost-effectiveness. Conclusion:Based on the domestic and foreign experiences, we suggest to further improve colorectal cancer screening programs in China, including designing evidence-based and feasible strategies, attaching importance to the implementation and management of the programs, and simultaneously implementing project monitoring and effectiveness evaluation.

11.
Acta Pharmaceutica Sinica ; (12): 2447-2455, 2021.
Article in Chinese | WPRIM | ID: wpr-886943

ABSTRACT

Type 2 diabetes is a complex metabolic disease, accompanied by insulin resistance and elevated blood glucose. As the disease progresses, hyperglucagonemia will occur. Glucagon has a significant effect on glucose increase and energy expenditure. In recent years, several glucagon receptor (GCGR) antagonists were developed. They lowered blood glucose in clinical studies, along with side effects, such as increased blood lipids and elevated liver transaminase. In order to solve these problems, glucagon like peptide 1 receptor (GLP-1R)/GCGR co-agonists were developed, which not only lower blood glucose, but also reduce weight and promote lipolysis. In this review, we will focus on the biological effects of glucagon, the treatments of GCGR antagonists, and GLP-1R/GCGR co-agonists on type 2 diabetes.

12.
Organ Transplantation ; (6): 563-2021.
Article in Chinese | WPRIM | ID: wpr-886785

ABSTRACT

Objective To evaluate the effect of bone marrow mesenchymal stem cell (BMSC) on the expression of interleukin (IL)-10 and tumor necrosis factor (TNF)-α in mice with ischemia-reperfusion acute kidney injury (IR-AKI). Methods All mice were randomly divided into the sham operation group (control group), ischemia-reperfusion injury group (IRI group) and BMSC treatment group (BMSC group), with 6 mice in each group, respectively. The renal function and pathological changes of mice were detected. The cell apoptosis of renal tissues of mice was determined. The expression levels of serum IL-10 and TNF-α of mice were quantitatively measured. The mouse BMSC was randomly divided into the control and hypoxia-reoxygenation groups (IRI group), and the expression levels of IL-10 and TNF-α in cell supernatant were determined. Results The renal structure of mice was normal in the control group, severe damage was observed in the IRI group, and mild damage occurred in the BMSC group. Compared with the control group, the renal tissue injury scores were significantly higher in the IRI and BMSC groups (both P < 0.05). Compared with the IRI group, the renal tissue injury score was significantly lower in the BMSC group (P < 0.05). Compared with the control group, the levels of serum creatinine (Scr) and blood urea nitrogen (BUN) were remarkably up-regulated in the IRI group, and the level of BUN was significantly up-regulated in the BMSC group (all P < 0.05). Compared with the IRI group, the levels of Scr and BUN were significantly down-regulated in the BMSC group (both P < 0.05). In the IRI group, the quantity of apoptotic cells in the renal tissues was considerably higher than those in the BMSC and control groups, and the quantity of apoptotic cells in the BMSC group was significantly higher than that in the control group (all P < 0.05). Compared with the control group, the levels of serum IL-10 and TNF-α were significantly up-regulated in the IRI group, whereas the level of serum TNF-α was significantly down-regulated and the level of serum IL-10 was significantly up-regulated in the BMSC group (all P < 0.05). Compared with the IRI group, the levels of serum IL-10 and TNF-α were significantly down-regulated in the BMSC group (both P < 0.05). The levels of IL-10 and TNF-α in the cell supernatant did not significantly differ between the IRI and control groups (P=0.080、0.627). Conclusions BMSC infusion may reduce the incidence of renal IRI and inflammation, probably via the mechanism of down-regulating TNF-α expression rather than up-regulating IL-10 expression.

13.
Protein & Cell ; (12): 261-278, 2021.
Article in English | WPRIM | ID: wpr-880901

ABSTRACT

TANK-binding kinase 1 (TBK1), a core kinase of antiviral pathways, activates the production of interferons (IFNs). It has been reported that deacetylation activates TBK1; however, the precise mechanism still remains to be uncovered. We show here that during the early stage of viral infection, the acetylation of TBK1 was increased, and the acetylation of TBK1 at Lys241 enhanced the recruitment of IRF3 to TBK1. HDAC3 directly deacetylated TBK1 at Lys241 and Lys692, which resulted in the activation of TBK1. Deacetylation at Lys241 and Lys692 was critical for the kinase activity and dimerization of TBK1 respectively. Using knockout cell lines and transgenic mice, we confirmed that a HDAC3 null mutant exhibited enhanced susceptibility to viral challenge via impaired production of type I IFNs. Furthermore, activated TBK1 phosphorylated HDAC3, which promoted the deacetylation activity of HDAC3 and formed a feedback loop. In this study, we illustrated the roles the acetylated and deacetylated forms of TBK1 play in antiviral innate responses and clarified the post-translational modulations involved in the interaction between TBK1 and HDAC3.

14.
Article in English | WPRIM | ID: wpr-880864

ABSTRACT

C18 ceramide plays an important role in the occurrence and development of oral squamous cell carcinoma. However, the function of ceramide synthase 1, a key enzyme in C18 ceramide synthesis, in oral squamous cell carcinoma is still unclear. The aim of our study was to investigate the relationship between ceramide synthase 1 and oral cancer. In this study, we found that the expression of ceramide synthase 1 was downregulated in oral cancer tissues and cell lines. In a mouse oral squamous cell carcinoma model induced by 4-nitroquinolin-1-oxide, ceramide synthase 1 knockout was associated with the severity of oral malignant transformation. Immunohistochemical studies showed significant upregulation of PCNA, MMP2, MMP9, and BCL2 expression and downregulation of BAX expression in the pathological hyperplastic area. In addition, ceramide synthase 1 knockdown promoted cell proliferation, migration, and invasion in vitro. Overexpression of CERS1 obtained the opposite effect. Ceramide synthase 1 knockdown caused endoplasmic reticulum stress and induced the VEGFA upregulation. Activating transcription factor 4 is responsible for ceramide synthase 1 knockdown caused VEGFA transcriptional upregulation. In addition, mild endoplasmic reticulum stress caused by ceramide synthase 1 knockdown could induce cisplatin resistance. Taken together, our study suggests that ceramide synthase 1 is downregulated in oral cancer and promotes the aggressiveness of oral squamous cell carcinoma and chemotherapeutic drug resistance.


Subject(s)
Animals , Apoptosis , Carcinoma, Squamous Cell , Cell Line, Tumor , Down-Regulation , Endoplasmic Reticulum Stress , Head and Neck Neoplasms , Mice , Mouth Neoplasms , Oxidoreductases
15.
Article in English | WPRIM | ID: wpr-880751

ABSTRACT

To overcome the computational burden of processing three-dimensional (3D) medical scans and the lack of spatial information in two-dimensional (2D) medical scans, a novel segmentation method was proposed that integrates the segmentation results of three densely connected 2D convolutional neural networks (2D-CNNs). In order to combine the low-level features and high-level features, we added densely connected blocks in the network structure design so that the low-level features will not be missed as the network layer increases during the learning process. Further, in order to resolve the problems of the blurred boundary of the glioma edema area, we superimposed and fused the T2-weighted fluid-attenuated inversion recovery (FLAIR) modal image and the T2-weighted (T2) modal image to enhance the edema section. For the loss function of network training, we improved the cross-entropy loss function to effectively avoid network over-fitting. On the Multimodal Brain Tumor Image Segmentation Challenge (BraTS) datasets, our method achieves dice similarity coefficient values of 0.84, 0.82, and 0.83 on the BraTS2018 training; 0.82, 0.85, and 0.83 on the BraTS2018 validation; and 0.81, 0.78, and 0.83 on the BraTS2013 testing in terms of whole tumors, tumor cores, and enhancing cores, respectively. Experimental results showed that the proposed method achieved promising accuracy and fast processing, demonstrating good potential for clinical medicine.

16.
Article in Chinese | WPRIM | ID: wpr-880079

ABSTRACT

OBJECTIVE@#To identify differentiation related miRNA and evaluate roles of miRNA during ATRA induced myeloid differentiation.@*METHODS@#The small RNA sequencing was used to analyze differential expressed miRNAs in ATRA induced NB4 cells. Then the several up or down-regulated miRNA were selected as the research candidates. SgRNAs targeting the genome of each miRNA were designed and NB4 cells with inducible expression of Cas9 protein were generated. After transduced sgRNA into NB4/Cas9 cells, the mutation level by PCR and surveyor assay were evaluated. The cell differentiation level was investigated by surface CD11b expression via flow cytometry.@*RESULTS@#A total of 410 mature miRNAs which expressed in NB4 cells were detected out after treated by ATRA, 74 miRNAs were up-regulated and 55 were down-regulated miRNAs with DNA cleavage generated by CRISPR/Cas9 was assayed directly by PCR or surveyor assay, quantitative PCR showed that the expression of miRNA was downregulated, which evaluated that gene edition successfully inhibitied the expression of mature miRNA. MiR-223 knockout showed the myeloid differentation of NB4 significantly inhibitied, while miRNA-155 knockout showed the myeloid differentation of NB4 cells significantly increased.@*CONCLUSION@#CRISPR/Cas9 is a powerful tool for gene editing and can lead to miRNA knockout. Knockouts of miR-223 and miR-155 have shown a differentiation-related phenotype, and the potential mechanism is the integrative regulation of target genes.


Subject(s)
CRISPR-Cas Systems , Cell Differentiation , Gene Editing , MicroRNAs/genetics , Sequence Analysis, RNA , Tretinoin
17.
Article in Chinese | WPRIM | ID: wpr-879862

ABSTRACT

OBJECTIVE@#To explore the risk factors for endotracheal intubation during resuscitation in the delivery room among very preterm infants.@*METHODS@#A retrospective analysis was performed for 455 very preterm infants who were admitted to the neonatal intensive care unit from January 2017 to December 2019. They were divided into an intubation group (@*RESULTS@#The intubation rate was 17.4% (79/455). Compared with the intubation group, the non-intubation group had significantly higher gestational age, birth weight, and rates of caesarean birth, delayed cord clamping (DCC), resuscitation quality improvement, regular use of antenatal glucocorticoids in mothers and premature rupture of membranes > 18 hours (@*CONCLUSIONS@#Very preterm infants with younger gestational age, birth weight < 750 g, maternal diabetes mellitus, placenta previa or placenta previa status may have a higher risk for endotracheal intubation after birth. The regular use of antenatal glucocorticoids and DCC can reduce the risk of intubation during resuscitation in very preterm infants.


Subject(s)
Delivery Rooms , Female , Gestational Age , Humans , Infant , Infant, Newborn , Infant, Premature , Intubation, Intratracheal , Pregnancy , Retrospective Studies , Risk Factors
18.
China Occupational Medicine ; (6): 523-528, 2021.
Article in Chinese | WPRIM | ID: wpr-923081

ABSTRACT

OBJECTIVE: To explore the effect of intervention adherence on the use of respiratory protective equipment(RPE) among migrant workers who occupationally exposed to organic solvents. METHODS: A total of 901 migrant workers from 60 small and medium-sized enterprises exposed to organic solvents in a district of Guangzhou City were selected as study subjects using three-arm cluster randomized controlled trial. The enterprises were divided into control group, intervention group, and combined intervention group. Migrant workers in the control group did not receive any intervention, while migrant workers in the intervention group received traditional health education and mobile health(mHealth) intervention, and migrant workers in the combined intervention group received interventions of the intervention group plus peer education. The use of RPE by the migrant workers was evaluated in these three groups in the last week, and at the sixth month. RESULTS: The adherence rates of the three mHealth intervention measures(following the WeChat official account of the project, joining the WeChat/QQ group of the project, and reading the provided messages from project team) in the combined intervention group were higher than that in the intervention group(74.8% vs 53.6%, 70.7% vs 41.8%, 78.5% vs 65.0%, all P<0.01). In the two intervention groups, the adherence rates of traditional health education(attending occupational health training, and browsing public welfare posters) were higher than that of mHealth intervention and/or peer education(all P<0.01). Logistic regression analysis showed that regardless of mHealth intervention, traditional health education or peer education, the high compliance subgroups of the two intervention groups were more likely to wear RPE all the time(all P<0.05) compared with the control group. The intervention effectiveness of the combined intervention group was better than that of the intervention group. CONCLUSION: Improving compliance with occupational health education interventions can promote the use of RPE among migrant workers. The implementation of peer education is beneficial to improve intervention compliance and intervention effect of migrant workers.

19.
Article in English | WPRIM | ID: wpr-922396

ABSTRACT

OBJECTIVES@#To study the effect of different maintenance doses of caffeine citrate on the success rate of ventilator weaning in very preterm infants (gestational age of ≤32 weeks) with respiratory distress syndrome (RDS).@*METHODS@#A total of 162 preterm infants with RDS who were admitted to the hospital from January 2016 to December 2018 were enrolled in this prospective trial. These infants had a gestational age of ≤32 weeks and required invasive mechanical ventilation. They were randomly divided into a high-dose caffeine group and a low-dose caffeine group, with 81 infants in each group. Within 6 hours after birth, both groups were given caffeine at a dose of 20 mg/kg. After 24 hours, the high- and low-dose caffeine groups were given caffeine at a maintenance dose of 10 mg/kg and 5 mg/kg, respectively. The two groups were compared in terms of re-intubation rate within 48 hours after ventilator weaning, durations of ventilation and oxygen therapy, enteral feeding, weight gain, and the incidence rates of complications and adverse reactions during hospitalization.@*RESULTS@#The high-dose caffeine group had a significantly lower re-intubation rate within 48 hours after ventilator weaning than the low-dose caffeine group (@*CONCLUSIONS@#A high maintenance dose of caffeine can safely and effectively reduce the incidence rate of apnea after ventilator weaning and the failure rate of ventilator weaning in RDS preterm infants with a gestational age of ≤32 weeks, and therefore, it holds promise for clinical application.


Subject(s)
Caffeine , Citrates , Humans , Infant , Infant, Newborn , Infant, Premature , Prospective Studies , Respiratory Distress Syndrome, Newborn/therapy , Ventilator Weaning
20.
Article in Chinese | WPRIM | ID: wpr-888057

ABSTRACT

Nucleic acid aptamers, broad-spectrum target-specific single-stranded oligonucleotides, serve as molecules in targeted therapy, targeted delivery and disease diagnosis for the treatment of tumor or microbial infection and clinical detection. Due to the existence of components in the use of traditional Chinese medicine(TCM), the target is difficult to concentrate and the specificity of treatment is poor. The effective components of TCM are toxic components, so a highly sensitive detection method is urgently needed to reduce the toxicity problem at the same time. The combined application of TCM and modern medical treatment strategy are difficult and cannot improve the therapeutic effect. Aptamers, advantageous in biosensors, aptamer-nanoparticles for targeted drug delivery, and aptamer-siRNA chimeras, are expected to connect Chinese medicinals with nanotechnology, diagnostic technology and combined therapies. We summarized the preparation, screening, and modification techniques of nucleic acid aptamers and the biomedical applications and advantages in therapy, targeting, and diagnosis, aiming at providing a reference for the in-depth research and development in TCM.


Subject(s)
Aptamers, Nucleotide , Drug Delivery Systems , Medicine, Chinese Traditional , Nucleic Acids , RNA, Small Interfering
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