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1.
China Pharmacy ; (12): 289-294, 2021.
Article in Chinese | WPRIM | ID: wpr-872679

ABSTRACT

OBJECTIVE:To establish the method for the content determination of inorganic elements in Cyperus rotundus ,and to compare the contents of 14 kinds of inorganic elements in C. rotundus from different producing areas ,and to provide theoretical basis for its quality control and high quality resources development . METHODS :The samples were processed by microwave digestion,and ICP-MS method was used to determine the contents of Na ,Mg,K,Ca,Mn,Fe,Ni,Cu,Zn,As,Se,Sr,Cd and Pb. SPSS 23.0 software were used for principal component analysis (PCA)and cluster analysis. RESULTS :The average contents of above 14 kinds of inorganic elements in C. rotundus from different producing areas were 168.62,753.71, 6 938.33,24.31,14.69,197.77,0.60,2.43,26.89,0.21,0.06,5.64,0.05,0.32 mg/kg,respectively. The results of PCA showed that the cumulative variance contribution rate of the first four principal components was 86.203%,which could reflect most of the information of the original data. C. rotundus from Shandong ,Jiangxi,Shanxi,Hubei and Yunnan ranked the top five places in terms of comprehensive score of inorganic element contents. The results of cluster analysis showed that the samples from 9 producing areas were clustered into 5 categories,showing the characteristics of clustering by producing area. From the perspective of inorganic elements ,the quality of C. rotundus from East China ,Central China ,North China and Southwest China was better than that from South China. CONCLUSIONS :Essential trace elements like Na ,Mg,K,Ca,Mn,Fe,Cu,Zn,Sr are rich in C. rotundus,and there are small amounts of Ni ,As,Se,Cd,Pb elements in it. The contents difference of inorganic elements in C. rotundus from different origins may related to the geographical area it belongs to.

2.
China Pharmacy ; (12): 2833-2840, 2020.
Article in Chinese | WPRIM | ID: wpr-837535

ABSTRACT

OBJECTIVE: To estab lish the method for simultaneous determination of the contents of cyperotundone , nootkatone,α-cyperone and aristolone in the volatile oil of Cyperus rotundus ,compare the content differences of 4 components in C. rotundus samples from different origins ,and to provide reference for germplasm screening ,development and utilization of the medicinal material. METHODS :The volatile oil was extracted from 46 batches of C. rotundus from 12 origins. The contents of cypermenone,nootkatone,α-cyperone and aristolone in volatile oil were determined by HPLC. The determination was performed on Kromasil C 18 column with mobile phase consisted of methanol-water (68∶32,V/V)at the flow rate of 1.0 mL/min;the column temperature was 30 ℃;the detection wavelength was set at 242 nm;the sample size was 20 μL. Using the contents of above 4 components as evaluation indexes ,radar image analysis ,cluster thermal map analysis and principal component analysis were performed for comparing the quality of C. rotundus from different origins. RESULTS :The results of content determination methodology investigation met relevant requirements ;the total contents of 4 components in volatile oil from C. rotundus from different origins ranged from 136.986 4 to 538.832 1 mg/g,of which the total content of samples from Yunnan was the highest (the average value was 476.059 2 mg/g). Radar image analysis results showed that the whole contour in the 4 origins of Guangdong , Jiangxi,Guangxi and Yunnan was large relatively and better balanced ,among which the samples from Yunnan had the largest overall contour and the best balance. The cluster thermal map analysis results showed that the samples from 12 origins could be grouped into 2 categories,the first category was from Hubei ,Jiangxi,Yunnan,Sichuan,Guangdong,Shandong,Henan and Shaanxi;the second category was from Guangxi ,Shanxi,Anhui and Hainan ;the quality of samples from the first category were better than that of samples from the second category. The principal component analysis results showed that the cumulative contribution rate of the first three principal components was 96.1%,and the samples from 12 origins were mainly clustered into two categories ,which was consistent with the results of cluster thermal map analysis. CONCLUSIONS :Established HPLC method can be used for simultaneous determination of cypermenone ,nootkatone,α-cyperone and aristolone in volatile oil of C. rotundus from different origins. Among the samples from 12 origins,the quality of medicinal material from Yunnan is better.

3.
Article in Chinese | WPRIM | ID: wpr-744472

ABSTRACT

Objective To explore the efficacy and safety of endoscopic nasal vestibule cysts,lip gingival trench path nasal vestibule excision and low temperature plasma radiofrequency ablation under nasal endoscope in the treatment of nasal vestibule cyst.Methods From January 2013 to December 2017,ninety patients with nasal vestibule cyst admitted in Taiyuan Central Hospital were selected. According to different surgical methods,the patients were divided into nasal endoscope nasal vestibule cyst group( A group,26 cases),cleft lip gingival vestibule cyst excision group(B group,36 cases),low temperature plasma radiofrequency ablation group under nasal endoscopy( C group, 28 cases). The efficacy and safety of the three types of operation were compared. Results The operation time [(15.5 ± 3.6) min] and wound healing time [( 2. 3 ± 0. 9 ) d] in A group and the operation time [( 16. 3 ± 4.5)min],wound healing time[(2.1 ± 0.5)d] in C group were all significantly shorter than those in B group,the differences were statistically significant(t=5.268,4.698,5.624,4.267,all P<0.05).The amount of bleeding in C group[(9.3 ± 0.6)mL] was the least,followed by A group[(12.2 ± 1.7)mL],and the difference among the three groups was statistically significant(t=8.256,7.254,all P<0.05).The postoperative wound VAS score among the three groups was statistically significant(F=12.674,P<0.001),and the VAS score in C group[(1.6 ± 0.7)points]<A group[(3.2 ± 1.5)points]<B group[(4.9 ± 2.3)points],the differences among the three groups were statistically significant(t=4.256,3.998,2.989,all P<0.05).The degree of facial swelling in C group was mild(39.29% ) and moderate(60.71% ),the differences were statistically significant compared with those in A group and B group(χ2 =5.226,11.651,all P<0.05).The incidence rate of complications in C group (0.00% ) was significantly lower than that in A group(11.54% ) and B group (16.67% ),the difference was statistically significant ( χ2 =3.934, P < 0.05).Conclusion Compared with the endoscopic nasal vestibule cysts, lip gingival trench path nasal vestibule excision,the low temperature plasma ablation under nasal endoscopy in the treatment of nasal vestibular cyst has the best efficacy,it is safe,less painful,and not easy to relapse.

4.
Article in Chinese | WPRIM | ID: wpr-744423

ABSTRACT

Objective To explore the clinical effect and safety of bedside hand jejunal nutrition tube placement in the treatment of severe acute pancreatitis. Methods The clinical data of 120 patients with severe acute pancreatitis treated in Dazhou Central Hospital from December 2014 to December 2016 were retrospectively analyzed. The patients in the control group(60 cases) were treated with nasogastric tube enteral nutrition on the basis of routine treatment. The patients in the observation group(60 cases) were treated with bedside bare hands on the basis of routine treatment, placement of jejunal feeding tube for jejunal nutrition treatment. The changes of gastrointestinal function,blood and urine amylase content,ICU stay time and the incidence of complications such as reflux and aspiration pneumonia were observed. Results In the observation group,the success rate was 96. 7% (52 / 60),and the catheterization time was (23. 5 ± 8. 4)min,without any adverse reactions. The recovery time of bowel sounds and anal defecation time of the observation group were (2. 01 ± 0. 47)d and (2. 69 ± 0. 42)d,respectively,which were significantly shorter than those of the control group[(3. 63 ± 1. 52)d and (3. 96 ± 1. 36)d],and the differences were statistically significant(t = 7. 887, 6. 911,all P < 0. 01). There was no statistically significant difference in serum amylase level between the two groups before treatment(t = 1. 364,P > 0. 05). After treatment,the serum amylase level in the observation group was (163 ± 104)IU/ L,which was lower than that in the control group[(302 ± 136)IU/ L](t = 6. 289,P < 0. 01). The recovery time of urinary amylase and the stay time in ICU in the observation group were (9. 28 ± 1. 25)d,(11. 24 ± 0. 84)d, respectively,which were shorter than those in the control group[(16. 32 ± 3. 26) d,(18. 35 ± 2. 42) d],and the differences were statistically significant(t = 15. 619,21. 499,all P < 0. 01). Conclusion Enteral nutrition by beside manual placement of jejunal feeding tube in the treatment of patients with severe acute pancreatitis is simple and safe. It can effectively promote the recovery of intestinal function and shorten the time for ICU. It is worthy of application in clinical practice.

5.
China Pharmacy ; (12): 671-676, 2019.
Article in Chinese | WPRIM | ID: wpr-817072

ABSTRACT

OBJECTIVE: To prepare standard decoction of fried Perilla frutescens seed and study the quality standard. METHODS: According to the preparation requirements of standard decoction, 17 batches of standard decoction of fried P. frutescens seed were prepared, and the yield of paste was calculated. HPLC method was used for quantitative analysis of rosmarinic acid in standard decoction of fried P. frutescens seed. The determination was performed on Agilent 5 TC-C18(2) column with mobile phase consisted of methanol-0.1% formic acid solution (40 ∶ 60, V/V) at the flow rate of 1.0 mL/min. The detection wavelength was set at 330 nm, and column temperature was 30 ℃. The sample size was 5 μL. The transfer rate was calculated. HPLC fingerprint was established for 17 batches of standard decoction of fried P. frutescens seed, and analyzed by using TCM Chromatogram Fingerprint Similarity Evaluation System (2012 edition). The chromatogram peaks were identified by comparing retention time of common peak. RESULTS: The yield of paste were 5.55%-9.75% in 17 batches of standard decoction of fried P. frutescens seed. The contents of rosmarinic acid were 0.44%-1.58%, and average content was 1.08%. The transfer rates of rosmarinic acid were 18.31%-34.32%, and average transfer rate was 25.42%. In HPLC fingerprints for 17 batches of standard decoction of P. frutescens seed, a total of 11 common peaks were identified, and the similarity was higher than 0.95. Five common peaks were identified, namely caffeic acid (peak 3),luteolin (peak 5), rosmarinic acid (peak 8) , luteolin (peak 9), apigenin (peak 10). CONCLUSIONS: The quality control method of standard decoction of fried P. frutescens seed is established, which can provide reference for the formulation of the quality standard of fried P. frutescens seed granules and related preparations.

6.
Chinese Journal of School Health ; (12): 1852-1854, 2019.
Article in Chinese | WPRIM | ID: wpr-815638

ABSTRACT

Objective@#To investigate the blood lead level of outpatient children and associated factors in Zhuzhou, and to offer targeted advice for the prevention and control of lead exposure.@*Methods@#The lead level in blood of 1 600 children aged ≤14 years old and the content of serum calcium, iron, zinc, magnesium, copper were tested, and the children and their parents were given a questionnaire regarding influencing factors of blood lead exposure.@*Results@#The average blood lead level of the children was (95.2±46.5)μg/L, The proportion of children with blood lead level ≥100 μg/L was 25.7%. The blood lead level between boys and girls had a statistical differences(Z=1.85, 2.85, P<0.05). The blood lead level was negatively correlated with serum calcium, iron, zinc, magnesium and copper(F=16.80,P<0.01). The risk factors for lead exposure included frequently drinking canned or bottled soft drinks, failing to wash one’s hands before meals, taking popcorn frequently, using coal for heat and for cooking, and constantly sucking fingers or biting fingernails (OR=2.12, 1.57, 1.46, 1.78, 3.24, P<0.01).@*Conclusion@#The blood lead levels of children in Zhuzhou is higher than national average level. We should strengthen environmental protection and behavioral interventions, and regularly monitor lead exposure among children.

7.
China Pharmacy ; (12): 621-624, 2018.
Article in Chinese | WPRIM | ID: wpr-704640

ABSTRACT

OBJECTIVE: To investigate the inhibition effect of total alkaloid, total phenolic acid and total volatile oil from Ligusticum chuanxiong on the contraction of the isolated uterine smooth muscle of rats, and to provide reference for clarifying the promoting blood circulation to restore menstrual flow traditional efficacy of L.chuanxiong. METHODS: The isolated uterine smooth muscle of rats were collected and soaked in Locke's solution. Using dimethyl sulfoxide as blank control, verapamil hydrochloride as positive control, the contraction curves of the isolated uterine smooth muscle in rats were recorded with PowerLab electrophysiolograph. Inhibitory effects of total alkaloid, total phenolic acid (mass concentrations of 0. 025, 0. 05, 0. 1 mg/mL) and total volatile oil (0. 04, 0. 08, 0. 16 mg/mL) on the contraction of the isolated uterine smooth muscle of rats induced by oxytocin were evaluated by observing the changes of contraction activity and contraction tension. Inhibitory rates of contraction activity and contraction tension of uterine smooth muscle were calculated. RESULTS: The contraction activity and contraction tension of uterine smooth muscle in rats were increased significantly after treated with oxytocin (P<0. 05 or P<0. 01). Different concentrations of 3 types of compounds from L. chuanxiong all decreased contraction activity and contraction tension of uterine smooth muscle significantly (P<0. 05 or P<0. 01). Compared with blank control, except for 0. 025 mg/mL total phenolic acid, the inhibitory rates of contraction activity and contraction tension of uterine smooth muscle were all decreased significantly (P<0. 05 or P<0. 01)after treated with other concentration drugs or components. CONCLUSIONS: The total alkaloid, total phenolic acid and total volatile oil from L. chuanxiong show certain inhibitory effect on the contraction of isolated uterine smooth muscle in rats, which provide reference for promoting blood circulation to restore menstrual flow use of L. chuanxiong.

8.
China Pharmacy ; (12): 2135-2137, 2016.
Article in Chinese | WPRIM | ID: wpr-504449

ABSTRACT

OBJECTIVE:To establish a method for the simultaneous content determination of berberine hydrochloride,phello-dendrine hydrochloride and magnoflorine in Phellodendron amurense decoction piece,and to campare the contents of the 3 ingredi-ents in different grades of P. amurense decoction piece. METHODS:HPLC was performed on the column of Phenomenex Luna C18 with mobile phase of acetonitrile-0.05 mol/L KH2PO4 (gradient elution) at a flow rate of 1 ml/min,the detection wavelength was 280 nm,the column temperature was 30 ℃,and injection volume was 5 μl. RESULTS:The linear ranges were 0.387 0-7.740 μg for berberine hydrochloride(r=0.999 9),0.044 4-0.888 0 μg for phellodendrine hydrochloride(r=0.999 8)and 0.048 0-0.960 0 μg for magnoflorine(r=0.999 9);RSDs of precision, stability and reproducibility tests were lower than 3%, recoveries were 95.61%-103.22%(RSD=2.80%,n=6),96.18%-102.80%(RSD=1.84%,n=6) and 97.93%-102.78%(RSD=1.84%,n=6). CONCLUSIONS:The method is simple and accurate,and can be used for the contents determination of berberine hydrochloride, phellodendrine hydrochloride and magnoflonine in P. amurense. The contents of berbenine hydrochloride and phellodendrine hydro-chloride in the first-grade decation piece are higher than those in the second-grade decoction piece,and the content of magnoflorine in both decoction pieces shows no discernible differences.

9.
Basic & Clinical Medicine ; (12): 17-21, 2015.
Article in Chinese | WPRIM | ID: wpr-481376

ABSTRACT

Objective To observe the damage of liver cells and to investigate the distribution and expression of glu-cose-regulated protein 78 (Glucose regulated protein78, GRP78/Bip) in liver tissue under the positive acceleration (+Gz) exposure.Methods Totally 24 wistar rats were randomly assigned to four groups:blank control,+6Gz,+9Gz and +12Gz.Each rat was clamped to the centrifuge arm , prone position, with the head of the rat facing the axis of the centrifuge for +Gz orientation.The onset rate was +0.5 Gz/s, which was used trapezoidal acceleration curve effect and controlled by computer .Blank control group rats were placed on the arm of centrifuge and under-went a process similar to that described above , but they were not exposed to acceleration .+6Gz group,+9Gz group and +12Gz group were subjected at peak time 3 min in animal centrifuge , acceleration rate 0.5 G/s, five times with interval 30 min between times.In addition, liver tissue of rats were respectively observed by H .E.staining.Mean while, plasma aspartate aminotransferase ( AST) and alanine aminotransferase ( ALT) were tested determine the damageofliverfunction.Results +GzaccelerationstressinjuryincreasedserumASTandALTlevel.Compared with the stress control, +9Gz group and +12Gz group significantly increased in plasma ALT and AST as compared with control group ( P<0.05 ) .+12 Gz stress induced the highest level in these groups .The level of ALT in+2 Gz group was higher than that in +6 Gz group ( P<0.05 ) .HE staining showed derangement of liver cells , irregular shape, the cell gap is not clear, vacuolar changes in +Gz groups, and with the increase of G value.Compared with the control group, the expression of GRP78/Bip was focused in the cytoplasm;the expression of GRP78 in the experimental group is higher than that in the control group ( P<0.05 ) .+12 Gz group was significantly higher than+6Gz group and the control group (P<0.05).The expression of GRP78/Bip in liver tissue increased with the in-creasing of G value levels;the expression level of GRP78/Bip in +12Gz and +9Gz groups were higher than that in +6Gz and control group (P<0.05).Conclusions There is positively related expression of GRP78/Bip, which was associated with exposure of increasing G values .

10.
Article in Chinese | WPRIM | ID: wpr-468848

ABSTRACT

Objective To evaluate the safety and efficacy of microwave tissue coagulation assisted laparoscopic hepatectomy for the treatment of hepatic tumors.Methods 29 patients underwent laparoscopic hepatectomy using microwave liver tissue coagulation from December 2011 to June 2014 in the General Air Force Hospital.All patients underwent intraoperative ultrasound guided microwave coagulation assisted laparoscopic local hepatectomy.Intraoperative blood loss,operation time,postoperative hospital stay and complications were observed.Results All the operations were successfully performed without conversion to open surgery.There were 18 cases of hepatocellular carcinoma (HCC),2 cases of intrahepatic cholangiocarcinoma,2 cases of hepatic metastases from the rectal carcinoma,5 cases of hepatic hemangioma,1 case of hepatic focal nodular hyperplasia and 1 case of hepatic cystadenoma.Drainage tubes were placed in 3 patients and removed 5-7 d after the operation.The operation time was 150 (125-210) miu,the median amount of intraoperative bleeding was 120 (30-250) ml,the postoperative hospital stay was 7 (5-10) d.All patients recovered well after the operation and no complications such as intraabdominal bleeding and bile leakage were observed.The cutting edges of liver cancer of these 22 cases were negative.The postoperative follow-up time of these 22 cases were 3-36 months and no tumor recurrence and metastasis were observed.Conclusions Laparoscopic liver resection using intraoperative ultrasound guided microwave hepatic tissue coagulation can effectively control the bleeding,improve the safety of laparoscopic liver resection,which makes it a safe and effective minimally invasive technique.

11.
Chinese Journal of Anesthesiology ; (12): 1365-1367, 2015.
Article in Chinese | WPRIM | ID: wpr-488745

ABSTRACT

Objective To evaluate the effects of different doses of dexmedetomidine administered intranasally on the median effective concentration (EC50) of sevoflurane inhibiting responses to laryngeal mask airway (LMA) insertion in the pediatric patients.Methods American Society of Anesthesiologists physical status Ⅰ or Ⅱ pediatric patients of both sexes, aged 1-3 yr, with body mass index of 20-25 kg/m2, scheduled for elective surgery under general anesthesia, were randomly divided into 3 groups: control group (group C) , dexmedetomidine 1 μg/kg group (group D1) , and dexmedetomidine 2 μg/kg group (group D2).In D1 and D2 groups, dexmedetomidine 1 and 2 μg/kg (in 1 ml of normal saline) were administered intranasally at 1 h before induction of anesthesia, respectively.Group C received the equal volume of normal saline administered intranasally.The children were separated from their parents at 45-60 min after intranasal administration and admitted to the operating room.The children inhaled 8% sevoflurane until they lost consciousness.After loss of consciousness, the end-tidal concentration of sevoflurane was determined using the modified Dixon's up-and-down method.The initial end-tidal concentration of sevoflurane was set at 2%.The concentration was increased/decreased by 0.2% in the next patient according to the response to LMA insertion.The patient's sedation status and with LMA acceptance were evaluated, and the patient's satisfaction with sedation and with LMA acceptance was recorded.Probit analysis was used to calculate the EC50 and 95% confidence interval of sevoflurane inhibiting responses to LMA insertion.Results The patient's satisfaction with sedation and with LMA acceptance was significantly higher in D1 and D2 groups than in group C, and in group D2 than in group D1 (P<0.05).The EC50 (95% confidence interval) of sevoflurane inhibiting responses to LMA insertion was 2.03% (1.91%-2.15%), 1.76% (1.65%-1.87%) and 1.63% (1.53%-1.73%) in C, D1 and D2 groups, respectively.The EC50 was significantly lower in D1 and D2 groups than in group C, and in group D2 than in group D1 (P<0.05).Conclusion Dexmedetomidine 1 and 2 μg/kg administered intranasally can decrease the EC50 of sevoflurane inhibiting responses to LMA insertion in the pediatric patients, and 2 μg/kg produces better efficacy.

12.
Article in Chinese | WPRIM | ID: wpr-570590

ABSTRACT

Objective To detect the expression of p16 and discuss the significance in genesis and development of cervical cancer. Methods FQ-PCR method was used to detect the expression levels of p16 mRNA in cervical cancer(CC),cervical intraepithelial neoplasia(CIN),and normal tissues.And in situ hybridization was used to detect HR-HPV in these tissues.Results The expres-sion levels of p16 were 0.79±0.34,0.70±0.36,0.26±0.21 in CC,CIN and normal cervical tissues,respectively.The difference was significant (P 0.05).The expression level of p16 in HR-HPV positive cases was significantly higher than that in HR-HPV negative cases (P <0.05).Conclusion p16 shows high expression level in cervical cancer,which is closely related to the infection of HR-HPV infection.It suggests that the high ex-pression of p16 play an important role in genesis and development of cervical cancer.

13.
Clinical Medicine of China ; (12): 830-832, 2011.
Article in Chinese | WPRIM | ID: wpr-416386

ABSTRACT

Objective To investigate the tracing value of drug-loaded nano-active carbon for lymphatic visualization and its potential application in the lymphadenectomy of gastric cancer. Methods Sixty-three patients with advanced gastric carcinoma were randomized into 2 groups:control group and tracing group. For the tracing group, 5-Fu-loaded nano-active carbon (NP-5-FU) suspension was intratumorally administered by preoperative gastroscope-guided or intra-operative injection. No tracing reagents was given in the control group. The numbers of removed lymph nodes and metastasis-involved lymph nodes as well as the lymph node staining were recorded and compared between the two groups. The adverse effects was monitored. Results The resected lymph nodes in the tracing group were much more than that of control group (43.73 ± 9. 25 vs. 25. 36 ± 4. 73, t = 3.82,P < 0. 05). For the N1, N2 and N3 lymph nodes, 27. 17 ± 5. 82,13.73 ± 4. 61 and 2. 83 ± 0. 15 per case of the tracing group were desected, while they were 14. 45 ± 2. 75,8. 27 ± 1.89, and 2. 64 ± 0. 11 per case in the control group,respectively (P < 0. 05 ). 26. 2% (211/842) of the lymph nodes in the tracing group showed black intensity,higher than that of unstain lymph nodes (4. 5% ,21/470,χ2 =95. 108,P <0. 05) and that in control group (16.9%, 142/837)( χ2 =7.485 ,P < 0.05, ). No local and systemic adverse reactions were observed for the local injection of nano-active carbon. No extension of operating time and increased complications occurred. Conclusion Local injection of nano-active carbon which was loaded with 5-FU around the tumor is a very useful measure to trace the lymph nodes and facilitate the lymphedetectomy and chemotherapy.

14.
Article in Chinese | WPRIM | ID: wpr-412496

ABSTRACT

Objective To examine the effect of voice cues on nonfluent aphasia patients'performance in reading idioms.Methods Very familiar idioms were presented to aphasia patients on a computer screen either with or without an accompanying voice cue.The patients were asked to say the last two words of each idiom.The number of errors with the last two words of the idioms wag used to compare the patients'performance under the two conditions.Results The mean correct score was 9.46±13.08 when the idioms were presented without a voice cue,but 13.61±6.06 with the voice cue.The difference was statistically significant.Conclusion Voice cues can improve the speaking of familiar idioms for nonfluent aphasia patients.

15.
Article in Chinese | WPRIM | ID: wpr-383186

ABSTRACT

Objective To determine whether attention allocation deficits contribute to nonfluent aphasia during orthography, phonology, and semantic processing with Chinese characters. Methods Sixteen patients and sixteen controls participated in single and dual tasks. In the single task participants were required to read Chinese characters silently and to make radical, semantic, and final sound judgments (for example, whether the charactersincluded the sound 'ang' ) simultaneously. During the dual task the only difference was that the participants wereasked to read the presented characters aloud and make the same three judgments. Reaction time and error rate were recorded and analysed. Results Reaction times in orthographic judgment were significantly longer for patients than for controls in both task setups. The error rate was also significantly higher. The patients' reaction times in makingphonological judgments in the dual task were significantly longer than in the single task, but there was no significantdifference in error rates between the patients and the controls. In making semantic judgments, neither reaction times nor error rates differed significantly with either the single or dual task protocol. Conclusion Attention allocation deficits play a key role in Chinese character orthographic and phonological processing for nonfluent aphasia sufferers.

16.
Article in Chinese | WPRIM | ID: wpr-260672

ABSTRACT

<p><b>OBJECTIVE</b>To establish the quality criteria for decoction pieces of salt Alpinia.</p><p><b>METHOD</b>Decoction pieces of salt Alpinia were measured with moisture, total ash, acid-insoluble ash, water-extract and volatile oils according to the procedures recorded in the Chinese Pharmacopoeia 2010. The content of Nootkatone was determined by HPLC, and NaCl, by chloridion electrode method.</p><p><b>RESULT</b>We obtained results of total ash, acid-insoluble ash, water-extract and volatile oils of 10 batches of decoction pieces of salt Alpinia moisture; Meanwhile we set the HPLC and chloridion electrode method.</p><p><b>CONCLUSION</b>This research established a fine quality standard for decoction pieces of salt Alpinia.</p>


Subject(s)
Alpinia , Chemistry , Calibration , Chromatography, High Pressure Liquid , Electrochemistry , Oils, Volatile , Quality Control , Salts , Chemistry , Solubility , Water , Chemistry
17.
Progress in Modern Biomedicine ; (24): 102-105, 2006.
Article in Chinese | WPRIM | ID: wpr-499134

ABSTRACT

The culture of Magnetospirillum magneticum WM-1 depends on several control factors that have great effect on the magnetic cells concentration. Investigation into the optimal culture conditions needs a large number of experiments. So it is desirable to minimize the number of experiments and maximize the information gained from them. The orthogonal design of experiments and mathematical statistical method are considered as effective methods to optimize the culture condition of magnetotactic bacteria WM-1 for high magnetic cells concentration. The effects of the four factors, such as pH value of medium, oxygen concentration of gas phase in the serum bottle, C:C (mtartaric acid: msuccinic acid) ratio and NaNO3 concentration, are simultaneously investigated by only sixteen experiments through the orthogonal design L16(44) method. The optimal culture condition is obtained. At the optimal culture condition (pH 7.0, a oxygen concentration 4.0%, C: C (mtartaric acid: msuccinic acid) ratio 1:2 and NaNO3 100 mg l-1), the magnetic cells concentration is promoted to 6.5×107 cells ml-1, approximately 8.3% higher than that under the initial conditions. The pH value of medium is very important factor for magnetic cells concentration. It can be proved that the orthogonal design of experiment is of 90% confidence. The results from the hysteresis of WM-1 shows that Hc = 230 Oe, Ms = 0.9 emu/g dry wt. Cells,and Mr / Ms = 0.50.

18.
Article in Chinese | WPRIM | ID: wpr-678623

ABSTRACT

Objective To determine the clinical characteristics of hospitalized patients who died of pulmonary thromboembolism (PTE) confirmed by autopsy. Methods The medical and autopsy records of 36 cases of autopsy proven fatal PTE in General Hospital of PLA from 1982 to 2001were reviewed. Cases of tumor embolism, fat embolism, and amniotic fluid embolism were excluded from the study. Results Six patients [16 7% (95% confidence interval, 6 4% to 32 8%)] were diagnosed as PTE prior to death. The diagnosis of PTE was significantly lower in patients with COPD or coronary artery disease. No risk factors were noted in only 4 patients (13 9%). Only a minority of patients had pleuritic pain or hemoptysis(8 3% and 11 1% respectively), while a significantly larger proportion of patients (25%, P

19.
Article in Chinese | WPRIM | ID: wpr-554227

ABSTRACT

10% was found in 5 patients (16%). Mesothelial hyperplasia was found to be significantly higher in patients with PTE than that in the control group (P

20.
Article in Chinese | WPRIM | ID: wpr-555931

ABSTRACT

Objective In the present study we examined the roles of three proinflammatory cytokines, i.e. tumor necrosis factor-?(TNF-?), interleukin 1?(IL-1?), and IL-8 in the early phase of APTE, and analyze the impact of these cytokines on the systemic circulation. Methods APTE was induced in 7 anesthetized dogs by injecting the thrombus of own blood into the right jugular vein. Seven animals receiving only warm sterile saline served as controls. Arterial serum samples were collected regularly (0h, 0.5h, 1h, 2h, 4h, 6h) and cytokine levels could be measured by radio-immunity assay. Results Introduction of clots into the pulmonary circulation caused significant increase in the mean pulmonary arterial pressure(from 99.1?5.7 to 66.8?15.3mmHg, P

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