Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 34
Filter
Add filters








Type of study
Year range
1.
Chinese Journal of School Health ; (12): 1415-1417, 2021.
Article in Chinese | WPRIM | ID: wpr-887387

ABSTRACT

Objective@#To understand school physician in primary and secondary schools in Yichang City, Hubei Province from 2019 to 2020, and to provide a reference for strengthening the school physician team in primary and secondary schools.@*Methods@#149 and 102 primary and secondary schools from 6 municipal districts in Yichang City, Hubei Province, were randomly selected in November 2019 and November 2020, respectively, and were administered by questionnaire survey.@*Results@#The proportion of school physicians increased from 39.6% in 2019 to 65.7% in 2020. In the past two years, the equipment rate of school physician in both central and fringe urban areas increased, especially the fringe urban areas, number of school physician increased from 38 to 96. A total of 93 and 141 school physicians were selected to pariticipate in questionaire survey in 2019 and 2020 respectively. The survey showed that more than 90% of school physicians in primary and secondary schools in Yichang received training, and 74.5% had college education level. However, most of them lack professional qualification and medical background.@*Conclusion@#School physician of primary and secondary of Yichang is well development over the past two years, and the proportion substantially increased. However, there is still room for improvement in the quantity and quality of school physicians, and professional qualification needs to be improved. More attention should be paid to the marginal urban areas to achieve a balance between quantity and quality.

2.
Article in English | WPRIM | ID: wpr-880869

ABSTRACT

Gap junction (GJ) has been indicated to have an intimate correlation with adhesion junction. However, the direct interaction between them partially remains elusive. In the current study, we aimed to elucidate the role of N-cadherin, one of the core components in adhesion junction, in mediating connexin 43, one of the functional constituents in gap junction, via transforming growth factor-β1(TGF-β1) induction in osteoblasts. We first elucidated the expressions of N-cadherin induced by TGF-β1 and also confirmed the upregulation of Cx43, and the enhancement of functional gap junctional intercellular communication (GJIC) triggered by TGF-β1 in both primary osteoblasts and MC3T3 cell line. Colocalization analysis and Co-IP experimentation showed that N-cadherin interacts with Cx43 at the site of cell-cell contact. Knockdown of N-cadherin by siRNA interference decreased the Cx43 expression and abolished the promoting effect of TGF-β1 on Cx43. Functional GJICs in living primary osteoblasts and MC3T3 cell line were also reduced. TGF-β1-induced increase in N-cadherin and Cx43 was via Smad3 activation, whereas knockdown of Smad3 signaling by using siRNA decreased the expressions of both N-cadherin and Cx43. Overall, these data indicate the direct interactions between N-cadherin and Cx43, and reveal the intervention of adhesion junction in functional gap junction in living osteoblasts.


Subject(s)
Cadherins , Cell Communication , Connexin 43 , Osteoblasts , Transforming Growth Factor beta1
3.
Article in Chinese | WPRIM | ID: wpr-880448

ABSTRACT

Biliary stent has been widely used in the treatment of biliary stricture and obstruction, it can relieve the pain of patients effectively, but bacterial infection and stent obstruction are still troublesome after surgery. We introduce the mechanism of infection and stent blockage caused by bacterial invasion after biliary stent implantation, and expound the formation mechanism of bacterial biofilm and bile sludge in this review. Antibacterial biliary stent is an effective way to inhibit biliary tract infection, the literatures on antibacterial modification of biliary stent with different antibacterial methods in domestic and abroad are reviewed, and the research prospect of antibacterial biliary stent is summarized and prospected.


Subject(s)
Anti-Bacterial Agents/pharmacology , Bile , Biliary Tract , Cholestasis , Humans , Stents
4.
Article in Chinese | WPRIM | ID: wpr-864553

ABSTRACT

Objective:To investigate the effect of health belief model-based intervention on post-traumatic stress disorder and post-traumatic growth of parents of children with malignant lymphoma.Methods:A total of 90 malignant lymphoma children parents were randomly divided into study group and control group, with 45 cases in each group. The control group received routine nursing method, the study group carried out health belief model-based intervention. The Posttraumatic stress Checklist-Civilian version (PCL-C) and Post-traumatic Growth Inventory (PTGI) were used to evaluate the effect of intervention, respectively.Results:After intervention, the scores of PLC-C were significantly decreased and PTGI were significantly increased both in the intervention group and control group. After intervention, the scores of arousal symptoms avoidance/numbing, re-experiencing symptoms and total PCL-C scores were (7.26±1.13), (8.80±1.95), (5.67±1.16), (21.73±2.57) points in the study group, which were significantly decreased compared to (8.09±1.63), (9.92±3.05), (6.10±0.68), (24.10±3.78) points in the control group ( t values were 2.070-3.484, all P<0.05 or 0.01). After intervention, the scores of relating to others, personal strength and total PTGI scores were (21.66±4.66), (18.28±5.04), (73.93±8.20) points in the study group, which were significantly increased compared to (18.39±4.39), (16.43±2.71), (66.46±6.20) points in the control group ( t values were 3.415, 2.161, 4.877, all P<0.05 or 0.01). Conclusion:Health belief model-based intervention can effectively alleviate post-traumatic stress disorder and promote posttraumatic growth in parents with malignant lymphoma children.

5.
Article in English | WPRIM | ID: wpr-828961

ABSTRACT

Homoeostasis depends on the close connection and intimate molecular exchange between extracellular, intracellular and intercellular networks. Intercellular communication is largely mediated by gap junctions (GJs), a type of specialized membrane contact composed of variable number of channels that enable direct communication between cells by allowing small molecules to pass directly into the cytoplasm of neighbouring cells. Although considerable evidence indicates that gap junctions contribute to the functions of many organs, such as the bone, intestine, kidney, heart, brain and nerve, less is known about their role in oral development and disease. In this review, the current progress in understanding the background of connexins and the functions of gap junctions in oral development and diseases is discussed. The homoeostasis of tooth and periodontal tissues, normal tooth and maxillofacial development, saliva secretion and the integrity of the oral mucosa depend on the proper function of gap junctions. Knowledge of this pattern of cell-cell communication is required for a better understanding of oral diseases. With the ever-increasing understanding of connexins in oral diseases, therapeutic strategies could be developed to target these membrane channels in various oral diseases and maxillofacial dysplasia.


Subject(s)
Bone and Bones , Cell Communication , Connexins , Metabolism , Physiology , Gap Junctions , Metabolism , Pathology , Homeostasis , Physiology , Humans , Mouth Diseases , Phosphorylation
6.
Chinese Journal of Biotechnology ; (12): 1293-1304, 2020.
Article in Chinese | WPRIM | ID: wpr-826847

ABSTRACT

Cordycepin as the main active ingredient of Cordyceps militaris, a traditional medicinal fungus in China, has many physiological functions such as anti-cancer, anti-tumor and anti-virus activity. The most potential route for effective cordycepin production has been considered as liquid fermentation of C. militaris though with low productivity at present. Thus, it is urgent to apply both process engineering strategy and metabolic engineering strategy to enhance the productivity of cordycepin. In this review, the effects of medium components (i.e. the carbon/nitrogen source, precursor substances and metal ions) and operation factors (i.e. pH, dissolved oxygen and light) on cordycepin biosynthesis in liquid fermentation system are summarized. Besides, separation of cordycepin, the gene cluster involved and predicted biosynthesis pathways of cordycepin are also discussed, providing possible solutions of finally realizing efficient production of cordycepin.


Subject(s)
Biotechnology , China , Cordyceps , Deoxyadenosines , Genetics , Fermentation , Metabolic Engineering
7.
China Pharmacy ; (12): 656-660, 2020.
Article in Chinese | WPRIM | ID: wpr-817540

ABSTRACT

OBJECTIVE:To establish the HP LC fing erprints of Oxalis corniculata and to simultaneously determine the contents of isovitexin and swertisin. METHODS :HPLC method was adopted. The determination was performed on ACE Excel- 5-C18column with mobile phase consisted of methanol- 0.1% phosphoric acid water (gradient elution )at the flow rate of 1 mL/min. The column temperature was 35 ℃,and detection wavelength was set at 280 nm. The sample size was 10 μL. Using isovitexin peak as reference,HPLC fingerprints of 12 batches of sample were drawn. The similarity evaluation was performed by using Similarity Evaluation System of Chromatogram Fingerprint of TCM (2012 edition)to determine common peaks. The contents of isovitexin and swertisin were determined by same method of above chromatogram. RESULTS :There were 19 common peaks in HPLC chromatogram of 12 batches of O. corniculata ,with the similarity above 0.89. Two common peaks including isovitexin and swertisin were identified. The linear range of two components were 3.91-117.36 μg/mL(r=0.999 4)and 9.88-118.56 μg/mL(r= 0.999 2),respectively. The limits of quantitation were 0.675 and 3.587 μg/mL;the limits of detection were 0.205 and 1.087 μg/mL, respectively. RSDs of precision ,stability and reproducibility tests were all lower than 2%. The recoveries were 95.46%-99.10% (RSD=1.23% ,n=6),95.34% -101.23%(RSD=2.74% ,n=6),respectively. The average contents were 0.227-1.654, 0.641-2.052 mg/g,respectively. CONCLUSIONS :Established fingerprints can be used for the quality control of O. corniculata ; the content determination method is simple and accu rate,and can be used for simultaneous determination of two components.

8.
Article in Chinese | WPRIM | ID: wpr-743617

ABSTRACT

Objective To describe the status of discharge readiness of patients with intracranial aneurysm undergoing interventional therapy and explore its influencing factors. Methods Easy sampling method was used to select 136 patients with intracranial aneurysm undergoing interventional therapy at the Department of Neurosurgery, Liaocheng People's Hospital. Clinical data questionnaire was applied to collect the clinical data of patients. Discharge readiness scale and discharge guidance quality scale were adopted to assess patients′ discharge readiness and discharge guidance quality. Pearson correlation analysis was used to analyze the correlation between discharge guidance quality and discharge readiness, while multi-linear regression analysis was used to analyze the influencing factors of discharge readiness. Results In this study, the total score of discharge readiness in patients with intracranial aneurysm undergoing interventional therapy was (157.90 ± 26.98) points, which was at a moderate level. Pearson correlation analysis showed that the discharge guidance quality was positively correlated with the discharge readiness for patients with intracranial aneurysm (r=0.619, P<0.01). Multi-linear regression analysis showed that the education level, family average monthly income, place of residence, numbers of long-term medications required, and discharge guidance quality were factors influencing the discharge readiness of patients with intracranial aneurysm undergoing interventional therapy (β′=0.102, 0.175, 0.391,- 0.226, 0.314, P<0.05). Conclusion In patients with intracranial aneurysm undergoing interventional therapy, the discharge readiness was at a moderate level, and the medical staff should make interventional strategies, taken the factors affecting the discharge readiness into consideration, and strength the discharge guidance quality to improve the patients′discharge readiness and prognosis.

9.
Chinese Journal of Lung Cancer ; (12): 289-298, 2019.
Article in Chinese | WPRIM | ID: wpr-775630

ABSTRACT

BACKGROUND@#Current research shows that platelet to lymphocyte ratio (PLR) has important prognostic value in renal cell carcinoma, esophageal cancer, gastric cancer, liver cancer and colon cancer. The aim of the study is to evaluate the prognostic value of PLR in non-small cell lung cancer (NSCLC) through meta-analysis.@*METHODS@#Literature search for PubMed, EMBASE, Web of Science, Medline, Cochrane Library, China National Knowledge Internet (CNKI), China Biomedical Medicine disc (CBMdisc), VIP, Wanfang Database using computer electronic system to study the association between PLR and overall survival (OS) and disease-free survival (DFS). Each eligible study data is extracted and a meta-analysis is performed using the hazard risk (HR) and 95% confidence interval (95%CI) to assess the prognostic value of PLR, the time limit for the search is to build the library until November 2018.@*RESULTS@#We include a total of 15 research literatures involving 5,524 patients for meta-analysis. According to the results of the meta-analysis: The OS of the higher PLR group is significantly lower than that of the lower PLR group (HR=1.69, 95%CI: 1.45-1.97, P<0.000,01, I²=46.2%, Pheterogeneity=0.026); the DFS of the higher PLR group is significantly lower than that of the lower PLR group (HR=1.41, 95%CI: 1.14-1.74, P=0.001, I²=46.2%, Pheterogeneity=0.026). Subgroup analysis show that the OS of the higher PLR group is still significantly lower than the lower PLR group (P<0.05) after grouping by ethnicity, sample size, PLR cutoff value and treatment.@*CONCLUSIONS@#Increased PLR is associated with poor prognosis in NSCLC, so PLR may be an important biological predictive marker for NSCLC patients, however, its clinical application still needs to be verified through more research in the future.


Subject(s)
Blood Platelets , Cell Biology , Carcinoma, Non-Small-Cell Lung , Blood , Diagnosis , Pathology , Humans , Lung Neoplasms , Blood , Diagnosis , Pathology , Lymphocytes , Cell Biology , Platelet Count , Prognosis
10.
Frontiers of Medicine ; (4): 229-237, 2019.
Article in English | WPRIM | ID: wpr-771312

ABSTRACT

This retrospective analysis aimed to investigate the mutation profile of 16 common mutated genes in de novo acute myeloid leukemia (AML) patients. A total of 259 patients who were diagnosed of de novo AML were enrolled in this study. Mutation profiling of 16 candidate genes were performed in bone marrow samples by using Sanger sequencing.We identified at least 1 mutation in 199 of the 259 samples (76.8%), and 2 or more mutations in 31.7% of samples. FLT3-ITD was the most common mutated gene (16.2%, 42/259), followed by CEBPA (15.1%, 39/259), NRAS (14.7%, 38/259), and NPM1 (13.5%, 35/259). Concurrence was observed in 97.1% of the NPM1 mutated cases and in 29.6% of the double mutated CEBPA cases. Distinct patterns of co-occurrence were observed for different hotspot mutations within the IDH2 gene: R140 mutations were associated with NPM1 and/or FLT3-ITD mutations, whereas R172 mutations co-occurred with DNMT3A mutations only. Concurrence was also observed in 86.6% of epigenetic regulation genes, most of which co-occurred with NPM1 mutations. The results showed certain rules in the mutation profiling and concurrence of AML patients, which was related to the function classification of genes. Defining the mutation spectrum and mutation pattern of AML will contribute to the comprehensive assessment of patients and identification of new therapeutic targets.


Subject(s)
Adolescent , Adult , Aged , CCAAT-Enhancer-Binding Proteins , Genetics , Child , Child, Preschool , China , DNA Mutational Analysis , Female , GTP Phosphohydrolases , Genetics , Gene Expression Profiling , Gene Frequency , Genetic Predisposition to Disease , Humans , Kaplan-Meier Estimate , Leukemia, Myeloid, Acute , Genetics , Male , Membrane Proteins , Genetics , Middle Aged , Mutation , Nuclear Proteins , Genetics , Phenotype , Retrospective Studies , Young Adult , fms-Like Tyrosine Kinase 3 , Genetics
11.
Article in Chinese | WPRIM | ID: wpr-805153

ABSTRACT

Objective@#To understand the situation of ticks carrying pathogens in border areas of Heilongjiang province.@*Methods@#From 2009 to 2018, tick specimens were collected in Yichun, Daxing′anling area and Jiamusi in Heilongjiang province. A total of 2 530 ticks were studied, including 800 Ixodes persulcatus and 1 730 Dermacentor silvarum. Tick-borne encephalitis virus (TBEV), severe fever with thrombocytopenia syndrome virus (SFTSV), omsk hemorrhagic fever virus (OHFV), langat virus (LGTV), powassan virus (POWV) were detected by real-time RT-PCR. Spotted fever group rickettsia (SFGR) and Borrelia burgdorferi sensulato (B.b.s.l) were detected by PCR in ticks collected from Jiamusi area.@*Results@#All tick speciments collected were negative for TBEV, SFTSV, OHFV, LGTV and POWV. Tick specimens from Jiamusi carried SFGR and B. b.s.l.with positive rates of 59.5% and 8.9%.@*Conclusions@#The ticks in border areas of Heilongjiang province carry spotted fever group rickettsia and Borrelia burgdorferi, and the carrying rate of spotted fever group rickettsia is high. The monitoring and control of ticks and tick-borne diseases should be strengthened.

12.
Article in Chinese | WPRIM | ID: wpr-753858

ABSTRACT

Objective To investigate the antimicrobial resistance of clinical bacterial isolates in Peking Union Medical College Hospital (PUMCH) in 2017. Methods A total of 9 515 non-duplicate clinical isolates were collected from January 1 to December 31, 2017. Disc diffusion test (Kirby-Bauer method) and E-test method were employed to determine antimicrobial susceptibility. Results Gram-negative bacilli and gram-positive cocci accounted for 68.2% and 31.8%, respectively among the 9 515 clinical isolates. Methicillin-resistant strains in S. aureus (MRSA) and coagulase-negative Staphylococcus (MRCNS) accounted for 25.6% and 73.3%, respectively. Extended-spectrum β-lactamases (ESBLs) -producing strains accounted for 47.6% (877/1 842), 27.6% (335/1 213) and 33.0% (59/179) in E. coli, Klebsiella spp (K. pneumoniae and K. oxytoca) and P. mirabilis, respectively. Enterbacteriaceae strains were still highly susceptible to carbapenems, with an overall resistance rate of ≤ 3.8%. The resistance rates of K. pneumoniae to imipenem and meropenem were 8.5% and 8.2%, respectively. About 72.7% and 70.4% of A. baumannii isolateswere resistant to imipenem and meropenem. The resistance rate of P. aeruginosa to imipenem and meropenem was 14.8% and 10.0%, respectively. The prevalence of extensively drug-resistant strains in A. baumannii, P. aeruginosa and K. pneumoniae was 31.7% (239/753), 1.0% (10/1 035), and 3.0% (33/1 117), respectively. Conclusions The common bacterialisolates show various level of resistance to antimicrobial agents. Laboratory staff should improve communication with clinicians to prevent the spread of resistant strains.

13.
Article in Chinese | WPRIM | ID: wpr-806647

ABSTRACT

Objective@#To analyze the epidemiological characteristics and distribution characteristics of tick-borne encephalitis in China in 2014, and to provide scientific basis for formulating specific prevention and control measures.@*Methods@#The epidemic data were obtained from the "infectious disease report information management system" , using Excel 2016, GIS and other software to summarize and analyze the cases of tick borne encephalitis (TBE) reported, using the number of cases, incidence, composition ratio and other indicators to analyze and describe the TBE epidemiological characteristics in China in 2014.@*Results@#In 2014, a total of 322 cases of TBE were reported in 9 provinces in China, with an annual incidence of 0.024/100, 000 and 1 death of patient. The provinces with high number of cases were Jilin province, Inner mongolia autonomous region and Heilongjiang province, and the number of cases in the other six provinces is no more than two. TBE was distributed in spring and summer, and it is concentrated in May to July. The age of the affected population was mostly concentrated in 40-49 years old, the male-female ratio was 1.6∶1 (198/124), and the patients were dominantly farmers, household and unemployed workers, and forestry workers, they accounted for 49.40% (159/322), 26.40% (85/322) and 18.60% (60/322) of the national TBE cases respectively. The three hospitals that reported the most TBE cases in 2014 were Inner mongolia forestry general hospital, Jiangyuan People′s hospital of Baishan city, Jilin province and Mudanjiang forestry central hospital of Heilongjiang province. The number of reported cases in these three hospitals accounted for 68.6% of the whole country. The laboratory diagnosis rate of Inner mongolia forestry general hospital was the highest (91.9%).@*Conclusions@#In 2014, the incidence of TBE in China has continued to rise compared with the previous two years. The geographical focus is mainly on the forest areas of Daxing′anling, Xiaoxing′anling and Changbai Mountain.

14.
Article in Chinese | WPRIM | ID: wpr-697488

ABSTRACT

Objective: To investigate the role of p38 MAPK pathway in IL-1β induced inflammatory secretory properties mesenchymal stem cells(SFMSCs) from human synovial fluid of temporomandibular joint. Methods: In vitro cultured hSFMSCs were divided into control group and IL-1β treated group(10 ng/ml IL-1β for 2 h), the activation of p38 MAPK was examined with western blot. Then, the gene expression and the secretion of IL-6 and IL-8 in control group, IL-1β treated group and p38 MAPK inhibitor pre-treated group(pretreatment with SB-203580 10 μmol/L prior to the addition of 10 ng/ml IL-1β) were examined by RT-PCR and cytometric bead array(CBA). Results: It was observed that the protein level of p-p38 MAPK, the secretion of IL-8 and IL-6 were increased in IL-1β treated group, and suppressed in p38 MAPK inhibitor pre-treated group. Conclusion: p38 MAPK pathway of SFMSCs might play a role in IL-6 and IL-8 secretion by SFMSCs in inflammatory environment.

15.
Journal of Clinical Surgery ; (12): 73-76, 2018.
Article in Chinese | WPRIM | ID: wpr-694984

ABSTRACT

With the development of medical technology,the treatment of breast cancer has en-tered into a comprehensive treatment age which combines chemotherapy,radiotherapy,endocrine therapy and molecular targeting therapy from the time of local treatment. Neoadjuvant therapy has been widely ap-plied in clinic due to many advantages,such as lowering the primary breast tumor and axillary lymph node,improving breast conserving rate and predicting drug sensitivity. With the emergence of molecular typing of breast cancer,neoadjuvant therapy has changed from primary neoadjuvant chemotherapy to the comprehensive treatment with neoadjuvant chemotherapy,neoadjuvant endocrine therapy and neoadjuvant targeted therapy. Here is a review of the progress of neoadjuvant therapy,evaluation of curative effect,se-lection of operation mode and challenges of neoadjuvant therapy from different molecular typing of breast cancer.

16.
Article in Chinese | WPRIM | ID: wpr-691227

ABSTRACT

Without losing its high resolution, high-speed atomic force microscope (HS-AFM) represents a perfect combinationof scanning speed and precision and allows real-time and observation of the dynamic processes in a biological system atboth the cellular and molecular levels. By combining the extremely high temporal resolution with the spatial resolution andcoupling with other advanced technologies, HS-AFM shows promising prospects for applications in life sciences such as cellbiology. In this review, we summarize the latest progress of HS-AFM in the field of cell biology, and discuss the impact ofenvironmental factors on conformation dynamics of DNA, the binding processes between DNA and protein, the domainchanges of membrane proteins, motility of myosin, and surface structure changes of living cells.

17.
Article in Chinese | WPRIM | ID: wpr-691203

ABSTRACT

Telomere plays a crucial role in the physiological and pathological processes of cells. At the end of the telomere, the single-stranded DNA repeat sequence rich in guanine (G) folds in the presence of monovalent metal ions such as Na or K to form a G-quadruplex structure. This structure can not be extended by telomerase and inhibits the activity of telomerase, thus becoming a potential anticancer target. Stabilizing the formation of DNA G-quadruplex structures by small molecule ligands has become a new strategy for designing many anticancer drugs, and studying the interaction strength of these small molecule ligands with G-quadruplex is thus of particular importance for screening highly effective anticancer drugs. Single molecule force spectroscopy enables direct measurement of the interaction between small molecule ligands and G-quadruplexes. This review highlights the advances of single-molecule force spectroscopy based on atomic force microscopy in the study of the G quadruplex structure and its interaction with small molecule ligands, and summarizes the application and development trend of single molecule force spectrum technology in G quadruplex.

18.
Acta Pharmaceutica Sinica B ; (6): 645-654, 2018.
Article in English | WPRIM | ID: wpr-690875

ABSTRACT

Herein, a strategy is proposed for the simultaneous determination of primary coumarins in Peucedani Radix (Chinese name: Qianhu). The methodology consists of three consecutive steps: 1) Semi-preparative LC in combination with a home-made automated fraction collection module to fragment the universal metabolome standard into ten fractions (Frs. I-X); 2) LC-accurate MS/MS and quantitative H NMR spectroscopy conducted in parallel to acquire the qualitative and quantitative data of each fraction; 3) Robust identification and quantification of components by use of LC coupled to multiple reaction monitoring. In this final step, the most significant fractions (Frs. III-X) were pooled to serve as the pseudo-mixed standard solution. Meticulous online parameter optimization was performed to obtain the optimal parameters, including ion transitions and collision energies. Concerns were particularly paid onto pursuing the parameters being capable of monitoring -specific isomers, notably praeruptorin E 3'-isovaleryl-4'-angeloylkhellactone. The quantitative performance of the method was validated according to diverse assays. Eleven primary coumarins (-) were unambiguously identified and absolutely quantified, even though no external reference compound was used. Above all, the integrated strategy not only provides a feasible pipeline for the quality assessment of Peucedani Radix, but more importantly, shows the potential for authentic compound-free quantitative evaluation of traditional Chinese medicines.

19.
Article in English | WPRIM | ID: wpr-327808

ABSTRACT

Objective To summarize our experiences in the diagnosis and prognosis of different subtypes of primary thyroid lymphoma (PTL). Methods The clinical data of 27 PTL patients who were treated in our hospital from January 1998 to December 2014 were retrospectively analyzed. The pathological types of these patients included B cell lymphoma unclassifiable (BCLU) (n=5),mucosa-associated lymphiod tissue lymphoma (MALT) (n=9),diffuse large B cell lymphoma (DLBCL) (n=12),and T cell lymphoma (n=1). Results Of all these 27 cases,the most common clinical symptom was painless swelling of the neck (n=21,77.8%). Of 7 patients who had received preoperative fine needle biopsy,lymphoma was suspected in 2 cases (28.6%). Among these 7 cases,the positive rate of suspicious lymphoma was 66.7% in 3 DLBCL patients,0 in 3 MALT patients,and 0 in 1 BCLU paitent. Also,25 patients underwent intraoperative frozen pathological examination,which revealed lymphoma or suspicious lymphoma in 16 cases (64.0%); in these patients,the positive rate was 66.7% for BCLU,77.8% for MALT,58.3% for DLBCL,and 0 for T-cell lymphomas. The overall survival was (89.3±12.4) months,and the overall 5-year survival rate was 61.6%. The estimated survival in symptomatic group was 31.6 months,which was significantly shorter than that in asymptomatic group (97.9 months) (P=0.032). Other factors including age,sex,tumor size,tumor stage,international prognostic index,tracheal compression,lactate dehydrogenase,residual tumor,and pathological type showed no significant effect on survival(all P>0.05). Conclusions DLBCL has the highest fine needle biopsy positive rate,MALT has the highest frozen pathological positive rate,and intraoperative frozen pathology has more malignant results than the preoperative fine needle biopsy in the diagnosis. The accompanying lymphoma symptoms may be an adverse prognostic factor.

20.
Article in Chinese | WPRIM | ID: wpr-610744

ABSTRACT

Objective To analyze the characteristics of Behcet's disease (BD) with intestinal lesions to provides further reference for the diagnosis and treatment.Methods Four hundred and one cases of BD patients' data were collected including basic data,colonoscopy report,pathology diagnosis and hematologic examination.Statistical description of intestinal lesions was applied and analyzed for the differences between the locations and blood examinations of intestinal lesions.Results Within these 401 BD patients,88 (21.95 %) with intestinal lesions.Intestinal ulcerations,including both active and inactive phase,appeared in 52 cases (12.97%) mostly founded in ileocecum,in which 62.86% (22/ 401,5.49% of all BD patients) without the abdominal discomfort.Compared with inactive phase,the active phase intestinal BD patients have higher inflammation indexes with C reactive protein (CRP) increased of statistical significance (P =0.028).Thirty-three cases (8.23 %) with adenomas were founded,including tubular adenoma,serrated adenoma and villous adenoma.Mostly occurred in rectum,sigmoid colon,especially the sigmoid colon rectum junction.Adenomas were seen in eider patients with statistical significance compared to BD patients without intestinal lesion or with intestinal ulceration (P =0.022,0.000),and exhibited elder changes of lab tests,such as increas in aspartate aminotransferase (AST),gamma-glutamyl transferase (GGT) and uric acid (UA).In addition,2 cases of melanosis coli and 1 case of caecal diverticulum were also founded.Conclusions BD patients with intestinal ulcer or adenoma takes a fairly high ratio,and most intestinal BD and BD with adenoma patients did not complaint related symptoms,so colonoscopy as a screening method for diagnosis and treatment of BD is necessary.

SELECTION OF CITATIONS
SEARCH DETAIL