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1.
Article in Chinese | WPRIM | ID: wpr-927921

ABSTRACT

The present study explored the effect and mechanism of repeatedly steamed and sundried Rehmanniae Radix Praeparata(RRP) in delaying brain aging in ovariectomized mice. After ovariectomy, the mice were randomly divided into a model group, an estradiol valerate group(0.3 mg·kg~(-1)), and low-(1.0 g·kg~(-1)), medium-(2.0 g·kg~(-1)), and high-dose(4.0 g·kg~(-1)) RRP groups, and a sham operation group was also set up, with 15 mice in each group. One week after the operation, intragastric administration was carried out for 15 consecutive weeks. The step-down test and Morris water maze test were used to detect the behavioral changes of mice. HE staining and Nissl staining were used to observe the morphological changes of mouse brain tissues. Immunohistochemistry was used to detect the expression of Aβ and ER_β in mouse brain tissues. The serum estrogen levels and cholinesterase and cholinesterase transferase levels in brain tissues of mice were detected by assay kits. The extracted hippocampal protein was detected by the Nano-ESI-LC-MS system, identified by the Protein Discovery, and analyzed quantitatively and qualitatively by the SIEVE. The PANTHER Classification System was used for GO analysis and KEGG pathway enrichment analysis of the differential proteins. Compared with the sham operation group, the model group showed decreased learning and memory ability, shortened step-down latency(P<0.05), prolonged escape latency(P<0.05), reduced platform crossings and residence time in the target quadrant, scattered nerve cells in the hippocampus with enlarged intercellular space, increased expression of Aβ-positive cells(P<0.05), declining expression of ER_β-positive cells and estrogen level(P<0.05), and weakened cholinergic function(P<0.05). Compared with the model group, the RRP groups showed improved learning and memory ability, prolonged step-down latency(P<0.05), increased estrogen level(P<0.05), neatly arranged nerve cells in the hippocampus with complete morphology, declining Aβ-positive cells, and elevated expression of ER_β-positive cells. A total of 146 differential proteins were screened out by proteomics, and KEGG pathway enrichment yielded 75 signaling pathways. The number of proteins involved in the dopaminergic synapse signaling pathway was the largest, with 13 proteins involved. In summary, RRP can delay brain aging presumedly by increasing the level of estrogen, mediating the dopaminergic synapse signaling pathway, and improving cholinergic function.


Subject(s)
Aging , Animals , Female , Hippocampus/metabolism , Learning , Mice , Plant Extracts , Proteomics , Rehmannia
2.
Article in Chinese | WPRIM | ID: wpr-880134

ABSTRACT

OBJECTIVE@#To investigate the relationship between single nucleotide polymorphisms (SNPs) of IKAROS family Zinc finger 3 (IKZF3) gene and the risk of acute lymphoblastic leukemia (ALL) in children.@*METHODS@#The peripheral blood samples from 286 children with ALL and 382 healthy children were collected and divided into ALL group and control group, respectively. The genotypes of IKZF3 gene at rs62066988 C > T and rs12946510 C > T were detected by quantitative PCR with TaqMan detection system, and their correlation with ALL was analyzed.@*RESULTS@#The distribution frequencies of CC, CT and TT genotypes at rs62066988 in ALL group were 58.39%, 37.06% and 4.55%, respectively, while those in control group were 69.19%, 27.68% and 3.13%, respectively. The distribution frequencies of CC, CT and TT genotypes at rs12946510 in ALL group were 58.16%, 34.75% and 7.09%, respectively, while those in control group were 55.76%, 37.43% and 6.81%, respectively. Compared with the control group, the distribution frequency of CT/TT genotype at rs62066988 was significantly increased in the ALL group (OR=1.59, 95%CI: 1.16-2.19, P=0.004). However, there was no significant difference in the distribution of rs12946510 C > T polymorphism between ALL group and control group.@*CONCLUSION@#The CT/TT genotype of IKZF3 at the site of rs62066988 is associated with the increased risk of ALL in children.


Subject(s)
Alleles , Case-Control Studies , Child , Gene Frequency , Genetic Predisposition to Disease , Genotype , Humans , Ikaros Transcription Factor/genetics , Polymorphism, Single Nucleotide , Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics
3.
Article in Chinese | WPRIM | ID: wpr-906086

ABSTRACT

Objective:To investigate the effect of licochalcone A (LCA) on apoptosis in human breast cancer MDA-MB-231 cells, and to explore its possible mechanism. Method:MDA-MB-231 cells were treated with LCA of different concentrations, and<italic> </italic>cell counting kit-8 (CCK-8) assay was used to detect the cell viability. The cells were treated with LCA (10, 20, and 40 μmol·L<sup>-1</sup>) for 24 h, and apoptosis was detected by Annexin V staining with fluorescein isothiocyanate (FITC) and propidium iodide (PI) (Annexin V-FITC/PI). The level of intracellular reactive oxygen species (ROS) was detected by 2′,7′-dichlorodihydrofluorescein diacetate (DCFA-DA) fluorescent probe. Mitochondrial membrane potential (MMP) was detected by 5, 5′, 6, 6′-tetrachloro-1, 1′, 3, 3′-tetraethyl-imidacarbocyanine (JC-1) fluorescence probe. Western blot was used to detect the expression of cell apoptosis-related proteins, such as B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (Bax), and endoplasmic reticulum (ER) stress-related proteins, such as C/EBP homologous protein (CHOP), activating transcription factor 4 (ATF4), protein kinase R-like ER kinase (PERK), p-PERK, eukaryotic translation initiation factor 2 alpha (eIF2<italic>α</italic>), and p-eIF2<italic>α</italic>. Result:With the increase in the drug concentration (starting from 5 μmol·L<sup>-1</sup>), the cell viability decreased (<italic>P<</italic>0.05) with IC<sub>50 </sub>of 19.05 μmol·L<sup>-1</sup> as compared with the normal group. Additionally, the apoptosis rates of the LCA groups (10, 20, 40 μmol·L<sup>-1</sup>) significantly increased (<italic>P</italic><0.05), which reached 30.2% (<italic>P</italic><0.05) at LCA concentration of 40 μmol·L<sup>-1</sup>. LCA (10, 20, and 40 μmol·L<sup>-1</sup>) decreased the expression of Bcl-2 (<italic>P<</italic>0.05) and increased Bax expression (<italic>P<</italic>0.05) in a dose-dependent manner. Besides, the intracellular ROS level was elevated (<italic>P<</italic>0.05) and mitochondrial MMP was reduced (<italic>P<</italic>0.05) after LCA (10, 20, and 40 μmol·L<sup>-1</sup>) treatment in a dose-dependent manner, leading to mitochondrial dysfunction. LCA (10, 20, and 40 μmol·L<sup>-1</sup>) induced ER stress to up-regulate the expression of CHOP, ATF4, p-PERK, and p-eIF2<italic>α</italic> (<italic>P<</italic>0.05) in a dose-dependent manner. Conclusion:LCA can induce MDA-MB-231 cell apoptosis by increasing intracellular ROS level and reducing MMP to trigger mitochondrial dysfunction and ER stress.

4.
Article in Chinese | WPRIM | ID: wpr-921802

ABSTRACT

This paper aims to systematically analyze the peptides and proteins from Asini Corii Colla(ACC) through shotgun proteomics. After high-pH reversed-phase fractionation, the proteins and peptides in the hydrolysate of ACC were further separated by nano LC-Q-Exactive-MS/MS under the following conditions: Thermo Scientific EASY column(100 μm×2 cm, 5 μm, C_(18)) as precolumn, Thermo Scientific EASY column(75 μm×100 mm, 3 μm, C_(18)) for solid phase extraction, gradient elution with 0.1% formic acid in water(mobile phase A) and 84% acetonitrile in water containing 0.1% formic acid(mobile phase B), and MS in positive ion mode. Based on Uniprot_Equus caballus, MS data, and literature, 2 291 peptides were identified from ACC by MaxQuant, with 255 Maillard reactions(AML, CML, CEL)-modified peptides identified for the first time. Through alignment, the peptides were found to belong to 678 equine proteins. In conclusion, the combination of nano LC-Q-Exactive-MS/MS and shotgun proteomics achieved rapid and accurate identification of the proteins and peptides in ACC, which provides the key information and new insights for further investigation of chemicals and effective substances in ACC.


Subject(s)
Animals , Chromatography, Liquid , Horses , Peptides , Proteins , Proteomics , Tandem Mass Spectrometry
5.
China Pharmacy ; (12): 2485-2491, 2021.
Article in Chinese | WPRIM | ID: wpr-887429

ABSTRACT

OBJECTIVE:To establish a m ethod for simultaneous determination of neoastilbin ,astilbin,neoisoastilbin,isoastilbin, quercitrin and engeletin in Engelhardia roxburghiana,and conduct multivariate statistical analysis. METHODS :HPLC-QAMS method was adopted. The determination was performed on Phenomenex SuperLu C 18 column with mobile phase consisted of acetonitrile-0.1% formic acid (19 ∶ 81,V/V)at the flow rate of 1.0 mL/min. The detection wavelengths were set at 254 nm (neoastilbin,astilbin,neoisoastilbin,isoastilbin,engeletin)and 291 nm(quercitrin). The column temperature was 30 ℃,and sample size was 10 μL. Using astilbin as internal substance,and the relative correction factors of other 5 factors were calculated. The contents of each component were calculated according to relative correction factor ,and were compared with the results of external standard method. SPSS 22.0 software was used for cluster analysis and principal component analysis. RESULTS :The linear range of neoastilbin ,astilbin,neoisoastilbin,isoastilbin,quercitrin and engeletin were 0.007-0.311,0.871-18.184,0.002-0.119, 0.052-1.251,0.105-2.202,0.020-2.319 μg(r>0.999),respectively. RSDs of precision ,reproducibility and stability (24 h)tests were all lower than 3%. The average recoveries were 97.32%,94.89%,97.15%,96.90%,97.52% and 97.53%(RSDs were 1.09% -2.60% ,n=6),respectively. The relative correction factors of neoastilbin ,neoisoastilbin,isoastilbin,quercitrin and engeletin were 1.252 6,1.198 3,0.958 6,0.807 1 and 1.138 1, respectively. The contents of neoastilbin , neoisoastilbin, qq.com isoastilbin,quercitrin and engeletin measured by QAMS were 0.394 2-2.067 2,0.139 1-0.804 7,2.864 8-8.554 8,4.581 2- 11.371 1,1.028 9-13.401 5 mg/g;the contents of neoastilbin , astilbin,neoisoastilbin,isoastilbin,quercitrin and engeletin were 0.367 2-1.925 3,46.361 1-126.342 1,0.138 1-0.798 8,2.966 2-8.857 8, 4.642 5-11.523 3,0.970 6-12.641 9 mg/g,respectively. Relative errors of two methods was lower than or equal to 3.55%. The results of cluster analysis showed that 9 batches of samples could be clustered into two categories ;S8 sample was one category and others were one category. The results of principal component analysis showed that accumulative contribution rate of former 2 principle components was 84.745%,and the results of sample classification were consistent with those of cluster analysis. CONCLUSIONS : The established HPLC-QAMS method is accurate ,feasible and repeatable ,and can be used for simultaneous determination of 6 flavonoids in E. roxburghiana ,and it can provide reference for quality control.

6.
Acta Pharmaceutica Sinica ; (12): 1497-1508, 2021.
Article in Chinese | WPRIM | ID: wpr-887087

ABSTRACT

Adulterants and counterfeits were found in some of the commercial traditional Chinese medicine (TCM) decoctions in Hongjin Xiaojie Jiaonang, Hongjin Xiaojie Pian, and Chaihuang Keli during the national drug sampling inspection. However, it was difficult to determine the species of the adulterants and counterfeits by conventional testing methods. Therefore, a total of 184 samples of the TCM decoctions and raw materials belong to the prescriptions of above mentioned traditional Chinese patent medicines, including Bupleuri Radix, Bajiaolian, Heimayi, and Shufuchong, were collected and authenticated by DNA barcoding technology. 111 ITS2 sequences were obtained from 115 commercial TCM decoctions and raw materials of Bupleuri Radix, among which 71 were Bupleurum chinense, three were B. scorzonerifolium, and 31 were closely related species in the same genus. In addition, counterfeits derived from different genera, such as Ailanthus altissima (one sample), Saposhnikovia divaricate (two samples), and Solidago decurrens (three samples), were also detected. 21 ITS2 sequences were obtained from 22 commercial TCM raw materials of Bajiaolian, among which 15 were Diphylleia sinensis and six were Dysosma versipellis and other species in genus Dysosma. For 22 Heimayi samples, PCR amplification of COI sequence was failed due to genomic DNA degradation. Among 38 Shufuchong samples, 24 COI sequences were obtained and only nine of them were the genuine species (Armadillidium vulgare) recorded in the Chinese Pharmacopoeia, 11 were Porcellio laevis, two were Mongoloniscus sinensis, and two samples could not be identified due to the limitation of database. This study demonstrates that DNA barcoding technology is suitable for the species authentication of the decoctions of traditional Chinese patent medicine prescription. It is a conductive way for the establishment of traceability system for the whole TCM industrial chain.

7.
Article in Chinese | WPRIM | ID: wpr-879831

ABSTRACT

OBJECTIVE@#To study the efficacy and safety of double plasma molecular absorption system (DPMAS) in the treatment of pediatric acute liver failure (PALF).@*METHODS@#A prospective analysis was performed on the medical data of children with PALF who were hospitalized in the Intensive Care Unit (ICU), Hunan Children's Hospital, from March 2018 to June 2020. The children were randomly divided into two groups:plasma exchange group (PE group) and DPMAS group (@*RESULTS@#Compared with the PE group, the DPMAS group had a significantly lower number of times of artificial liver support therapy and a significantly shorter duration of ICU stay (@*CONCLUSIONS@#DPMAS is safe and effective in the treatment of PALF and can thus be used as an alternative to artificial liver support therapy.


Subject(s)
Adsorption , Child , Humans , Liver Failure, Acute/therapy , Plasma , Plasma Exchange , Prospective Studies
8.
Article in Chinese | WPRIM | ID: wpr-879004

ABSTRACT

Rhei Radix et Rhizoma is a kind of commonly used Chinese medicinal materials. Due to the overharvesting, the wild resource is endangering. Large market demand caused severely adulterant of commercial Rhei Radix et Rhizoma medicinal materials and decoction pieces. This manuscript reviewed the advances of the original species authentication in the industrial chain of Rhei Radix et Rhizoma during the latest decade, including characteristics and microscopic features, phytochemical analysis on anthraquinones, and molecular authentication based on DNA barcoding. Accordingly, an original species authentication route for the industrial chain of Rhei Radix et Rhizoma was summarized:(1)the identification of seeds and seedlings by DNA barcoding;(2) the selection of high variable sites based on the chloroplast genome;(3)biomonitoring of the Rhei Radix et Rhizoma medicinal materials and decoction pieces by two-dimensional DNA barcode;(4)traceability of Chinese patent medicines by third-generation sequencing. In conclusion, the combination of molecular identification and traditional identification methods provides a new idea for the identification of the original species of Rhei Radix et Rhizoma in the industrial chain and a essential guidance for the research of drug safety and efficacy of Rhei Radix et Rhizoma.


Subject(s)
Animals , Anthraquinones , Drugs, Chinese Herbal , Plant Roots , Rheum , Rhizome
9.
Article in Chinese | WPRIM | ID: wpr-824955

ABSTRACT

Objective: To investigate the effects of electroacupuncture (EA) on the behaviors of rat with anxiety disorder, and the expressions of hippocampal neurotransmitters including 5-hydroxytryptamine (5-HT), norepinephrine (NE) and dopamine (DA), and the expressions of hippocampal B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X (Bax).Methods: Forty-six male Wistar rats were randomly divided into a control group (n=10), a model group (n=12), an EA group (n=12), and a drug group (n=12). Except the control group, the other three groups were established into rat models of anxiety disorder using uncertain empty bottle stimulation. Rats in the EA group and the drug group received corresponding interventions for 15 consecutive days [EA group was given EA at Baihui (GV 20) and Sanyinjiao (SP 6); the drug group was given aqueous solution of alprazolam via intragastric administration]. After intervention, all four groups received open-field test (OFT) and elevated plus-maze (EPM) for behavioral evaluations. The expressions of 5-HT, NE and DA in hippocampus were determined by fluorescence spectroscopy (FS) while the expressions of Bcl-2 and Bax proteins in hippocampus were determined by Western blot (WB). Results: The OFT horizontal scores in the control group, EA group and drug group were significantly higher than that in the model group (all P<0.05), and the difference between the EA group and the drug group was statistically insignificant (P>0.05); the OFT vertical scores in the model group, EA group and drug group were significantly lower than the score in the control group (all P<0.05). The EPM percent of open-arm entries (OE%) in the control group, EA group and drug group was higher than that in the model group (P<0.05), and the differences among these three groups were statistically insignificant (P>0.05); though the percent of open-arm total time (OT%) in the EA group was lower than that in the control group (P<0.05), the difference was statistically insignificant when compared with the drug group (P>0.05), and it was significantly higher than that in the model group (P<0.05). The expression of 5-HT in the EA group was higher than that in the control group (P<0.05); the expression of 5-HT in the EA group was significantly lower than that in the model group (P<0.05); the difference between the EA group and the drug group was statistically insignificantly (P>0.05). The expression of NE in the model group was significantly higher than that in the other three groups (P<0.05), and there was no significant difference among these three groups (P>0.05). The expression of DA in the EA group was significantly higher than that in the control group and the drug group (both P<0.05), while the difference between the EA group and the model group was statistically insignificant (P>0.05). The expression of Bax in the model group was significantly higher than that in the other three groups (all P<0.05), whereas the expression of Bcl-2 in the model group was significantly lower than that in the other three groups (all P<0.05), and the differences in both Bax and Bcl-2 among the other three groups were statistically insignificant (all P>0.05). Bax/Bcl-2 in the EA group was significantly higher than that in the control group (P<0.05) and lower than that in the model group (P<0.05), and the difference was statistically insignificant when compared with the drug group (P>0.05). Conclusion: EA shows promising effects in attenuating rats' anxiety disorder, which may be achieved by the down-regulation of the expressions of 5-HT and NE in the hippocampus and/or inhibition of hippocampal neuronal apoptosis. The efficacy is comparable to that of intervention with alprazolam.

10.
Article in Chinese | WPRIM | ID: wpr-873502

ABSTRACT

@#Objective To analyze urine volume and dietary salt intake assessed by two 24 h urine specimens,to discuss its application value for evaluating population level and individual level dietary salt intake. Methods The subjects aged 18-69 years were selected from four counties in Shandong Province and Jiangsu Province from 2013 to 2014 by using multi-stage stratified cluster random sampling method. A questionnaire survey was conducted and two 24 h urine with an interval of one day were collected. The differences between two 24 h urine volume,and the dietary salt intake between two 24 h urine were assessed at individual level and population level. Results A total of 1 288 subjects was( 42.3± 14.0) years old,of which 626( 48.6%) were males. The average urine volume of the subjects was ( 1 462±437) ml. The first 24 h urine volume ( 1 427±488) ml was lower than the second 24 h urine volume ( 1 498± 552) ml ( t = -4.439,P<0.001) . The dietary salt intake was ( 9.8±3.3) g,and the dietary salt intake of males ( 10.1±3.5) g was higher than that of females ( 9.5±3.1) g ( t = 3.09,P= 0.002) . There was a significant difference in dietary salt intake among different age groups ( F= 7.57,P<0.001) . The dietary saltintake of 1 136 ( 88.2%) subjects was higher than the recommended level. At the individual level,the participants with the absolute difference with 1g between the subjects’two 24 h urinary salt excretion was 279 ( 21.7%) ,and the participants with the absolute difference with >3 g was 48.5%. At the population level,the first 24 h urinary salt excretions ( 9.9 ± 4.1) g was similar to that in the second 24 h urine and ( 9.7±4.0) g ( P= 0.1021) . The intraclass correlation coefficients between salt excretions in the two 24 h urine was 0.508( 95% CI: 0.451-0.559) . Conclusion The results of this study suggest that 24 h urinary salt excretion can better assess the salt intake at population level,but cannot accurately reflect the individual’s salt intake.

11.
Article in Chinese | WPRIM | ID: wpr-873308

ABSTRACT

Objective::To explore the effect of Erjingwan on the biological basis of kidney yin deficiency Alzheimer' s disease(AD)rats induced by ovariectomy+ D-galactose combined with amyloid beta1-40 (Aβ1-40). Method::After ovariectomy, rats were randomly divided into five groups: model group, positive group, Erjingwan high, medium and low dose group, 11 rats in each group, and 11 rats in sham operation group. One week after operation, D-galactose was injected intraperitoneally for 7 weeks, and four weeks after operation, Aβ1-40 was injected unilaterally into hippocampus. The rats in model group and sham-operation group were given saline by intragastric administration 3 weeks after operation. The rats in high, middle and low dose groups of Erjingwan were given corresponding concentration (9.0, 4.5, 2.25 g·kg-1). The rats in positive control group were given Donepezil 1.0 mg·kg-1 with dosage of 10 mL·kg-1 once a day for 35 consecutive days. After 30 days of administration, the learning ability of the rats were examined using a Y-maze. The hippocampus tissues of rats were isolated. The morphology of hippocampus was observed by Nissl staining.The proteins were detected by Nanol-ESI liquid-mass spectrometry system and identified by protein Discovery software. Relative quantitative and qualitative analysis of differential proteins in hippocampus was performed by SIEVE software, and Gene Ontology of differential protein was performed by PANTHER Classification System database. String analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment were performed on the differential proteins. Result::Compared with model group, the correct rate of Y maze in the high and middle dose groups of Erjingwan was significantly raised(P<0.05), and the number of neurons in the hippocampal CA1 area was significantly increased(P<0.01).115 differential proteins (Ratio>1.5 or Ratio<0.5) such as Insulin-like growth factor 1 receptors(IGF-1R) were found in the high-dose group of the Erjingwan group as well as 94 differential proteins such as Synaptophysin expressed in the middle-dose group of the Erjingwan. And there are 87 differential proteins such as Acetyl-CoA acetyltransferase-cytosolic in the positive drug group. It showed that these proteins were mainly divided into tubule-related proteins, heat shock proteins, energy metabolism-related proteins and AD-related proteins with GO analysis. It was found that the above differential proteins involved 93 signaling pathways such as Dopaminergic synaps by KEGG analysis. Conclusion::Erjingwan can improve cognitive impairment and neuronal damage in rats with kidney yin deficiency, possibly by altering the expression of multiple pathway-associated proteins such as phosphatidylinositol 3-kinase/protein kinase B signaling pathway(PI3K/Akt), insulin signaling pathway, and adenylate-activated protein kinase (AMPK)signaling pathway, estrogen signaling pathway, and Dopaminergic synapse.

12.
Article in Chinese | WPRIM | ID: wpr-872418

ABSTRACT

Objective: To investigate the effect of electroacupuncture (EA) on cognitive function in D-galactose (D-gal)-induced aging rats, and the correlation between the effect and nucleotide-binding oligomerization domain (NOD)-like receptor protein 3 (NLRP3)-ASC-Caspase-1 signaling pathway. Methods: Forty-six male Sprague-Dawley (SD) rats were randomly divided into a control group (n=10), a model group (n=12), an EA-7 d group (n=12) and an EA-21 d group (n=12). Except the control group, the other three groups received 42 consecutive days of intraperitoneal injection of D-gal to establish aging rat models with cognitive dysfunction. The control group received the same amount of normal saline via intraperitoneal injection. Two EA groups were given EA therapy for 21 consecutive days (began from the 22nd day of modeling) or 7 consecutive days (began from the 36th day of modeling) accordingly at Dazhui (GV 14), Baihui (GV 20), Shenshu (BL 23) and Zusanli (ST 36). After modeling/ intervention, all four groups received behavioral evaluations by Morris water maze (MWM) test, novel object recognition (NOR) test and step-down passive avoidance (SDPA) test followed by the Western blot (WB) detection of the expression levels of hippocampal NLRP3 inflammasome-associated proteins NLRP3, ASC and Caspase-1. Results: MWM (place navigation test, PNT) results showed that the escape latency in the model group was significantly longer than that in the other three groups (P<0.05), and there was no significant difference among the other three groups on the 1st day of the test (P>0.05). From the 2nd day to the 4th day of the test, there was no significant difference between the EA-21 d group and the control group (P>0.05) in the escape latency; the escape latency was significantly shorter in the EA-21 d group than in the model group and the EA-7 d group (P<0.05). MWM (spatial probe test, SPT) results showed that the time spent in the target quadrant was significantly shorter and platform crossover number was significantly lower in the model group than in the other three groups (P<0.05). The time spent in the target quadrant was longer in the EA-7 d group than in the model group (P<0.05), but was shorter than that in the control group and the EA-21 d group (P<0.05). There was no significant difference in the swimming speed among the four groups (P>0.05). NOR results showed that there was no significant difference in the recognition ratio between the EA-7 d group and the EA-21 d group (P>0.05), and the recognition ratio was significantly higher in the two EA groups than in the model group (P<0.05), but was lower than in the control group (P<0.05). SDPA results showed that the electric shock number was higher in the model group than in the other three groups (P<0.05), and the differences among the other three groups were statistically insignificant (P>0.05). The model group had the shortest step-down latency, followed by the EA-7 d group, the EA-21 d group and the control group in order (P<0.05). The WB results indicated that the expression level of NLRP3 was significantly lower in the control group and the EA-21 d group than in the model group and the EA-7 d group (P<0.05). The expression levels of ASC and Caspase-1 were significantly higher in the model group than in the other three groups (P<0.05), and there was no significant difference among these three groups (P>0.05). Conclusion: NLRP3 inflammasome may be involved in the development of cognitive decline in aging rats; 7 consecutive days of EA intervention can partially improve the cognitive impairment in aging rats though the effect is rather limited; 21 consecutive days of EA intervention may improve the learning and memory abilities in aging rats via downregulating the expression levels of NLRP3 inflammasome-associated proteins in hippocampus.

13.
Article in Chinese | WPRIM | ID: wpr-866723

ABSTRACT

Objective:To analyze the clinical distribution characteristics and drug resistance of Staphylococcus aureus infection and provide clinical basis for rational use of antibacterial drugs.Methods:From 2016 to 2019, 462 strains of Staphylococcus aureus were collected from sputum specimens of hospitalized children in Jingzhou Central Hospital.The clinical distribution characteristics of Staphylococcus aureus infection and drug resistance of various antibacterial drugs were analyzed.Results:Among 462 strains of Staphylococcus aureus infection, 285 strains were male, accounting for 61.7%, 177 strains were female, accounting for 38.3%.There was no statisticall significant difference between male and female children with Staphylococcus aureus pneumonia(χ 2=0.762, P=0.383). Staphylococcus aureus mainly infected children under 1 year old(χ 2=73.163, P<0.001), and the seasonal distribution was mainly in winter and spring(χ 2=27.656, P<0.001). A total of 462 strains of Staphylococcus aureus were detected, of which 363 strains(78.6%) were methicillin-sensitive Staphylococcus aureus(MSSA), and 99 strains(21.4%) were methicillin-resistant Staphylococcus aureus(MRSA). MSSA and MRSA were 100% sensitive to vancomycin, tigecycline, teicoplanin, rifampicin, linezolid, quinoprotein-daffodil and nitrofurantoin.The sensitivity of MSSA to levofloxacin, gentamicin, compound sulfamethoxazole, oxacillin, moxifloxacin, ciprofloxacin and tetracycline were 100.0%, 95.9%, 91.7%, 72.2%, 96.7%, 92.6% and 90.0%, respectively.The sensitivity of MRSA to levofloxacin, gentamicin, compound sulfamethoxazole, oxacillin, rifampicin, moxifloxacin, ciprofloxacin and tetracycline were 91.9%, 82.8%, 80.8%, 57.6%, 90.9%, 77.8% and 76.8%, respectively, and it suggested that the drug resistance rate of MRSA was higher than MSSA, and there was statistically significant difference.The antibiotics with high MSSA resistance rate were penicillin G(91.2%), erythromycin(61.7%), clindamycin(50.1%), MRSA was 100.0% resistant to penicillin G, erythromycin and clindamycin. Conclusion:The situation of clinical infection of Staphylococcus aureus in children is grim, and the Staphylococcus aureus detected in the specimens of respiratory tract infection in children is mainly MSSA, which has good sensitivity to most antibacterial drugs.MRSA and MSSA have obvious differences in drug resistance to various antibacterial drugs.Antibiotics should be used rationally, and nosocomial infection control should be strengthened to prevent and reduce the occurrence of MRSA.

14.
Article in Chinese | WPRIM | ID: wpr-864462

ABSTRACT

Objective:To compare the effect of the 2% chlorhexidinegluconate disinfectant and 0.5% iodophor disinfectant on the maintenance of central venous catheter (CVC) in patients with immunodeficiency.Methods:The patients were randomly divided into two groups. 2% chlorhexidinegluconate disinfectant was used in the experimental group, and 0.5% iodophor disinfectant was used in the control group. The CVC was sterilized once a week and continuously maintained for 3 weeks. According to the condition of catheter, the dressing was changed in time in case of any problem,and the reason and time for the catheter nursing care were recorded. The duration of changing the catheter dressing in different disinfectant, incidence rate of dressings looseness and incidence of catheter-related infections in two groups were analyzed.Results:The duration of skin drying and duration of caring in the experimental group were (0.62±0.10)min, (11.67±0.33) min, significantly shorter than the control group [(1.97±0.18) min, (14.48±0.33) min]( t values were 63.044, 57.296, P<0.01).In term of the adverse effects on CVC,there were no statistical differences between two groups ( P> 0.05). Conclusions:Both 2% chlorhexidinegluconate and 0.5% iodophor disinfectant have similar disinfection effect on CVC caring, but 2% chlorhexidinegluconate disinfection is more efficient and worthy of application in clinical settings.

15.
Chinese Pharmaceutical Journal ; (24): 465-472, 2020.
Article in Chinese | WPRIM | ID: wpr-857756

ABSTRACT

OBJECTIVE: To optimize and improve the quality standard of Qingkailing dropping pills. METHODS Lonicerae Japonicae Flos, geniposide in Gardeniae Fructus and baicalin in Scutellariae Radix of Qingkailing dropping pills were identified by TLC and HPLC respectively. Cholic acid and hydrodeoxycholic acid were determined by HPLC equipped with an ELSD and a column (4.6 mm×250 mm, 5 μm) packed with ODS bonded silica gel (5 μm particle size). The mobile phase was a mixture of methanol, acetonitrile and 0.1% formic acid (68∶17∶15, V/V/V) and the flow rate was 1.0 mL•min-1. Geniposide in Gardeniae Fructus of Qingkailing dropping pills was determined by HPLC equipped with a DAD (238 nm) and a column (4.6 mm×250 mm) packed with ODS bonded silica gel (5 μm particle size). The mobile phase was a mixture of acetonitrile and 0.05% phosphoric acid (10∶90, V/V) and the flow rate was 1.0 mL•min-1. Baicalin in Scutellariae Radix of Qingkailing dropping pills was determined by HPLC equipped with a DAD (275 nm) and a column (4.6 mm×150 mm) packed with ODS bonded silica gel (5 μm particle size), the mobile phase was a mixture of methnol, water and phosphoric acid (47∶53∶0.2, V/V/V), and the flow rate was 1.0 mL•min-1. RESULTS: The developed TLC spots were clear with good reproducibility. Hydrodeoxycholic acid, cholic acid, geniposide and baicalin showed good linear relationship in the ranges of 0.505 0-6.312 μg, 0.515-6.440 μg, 0.029 12-0.582 4 μg, and 0.109 2-1.092 μg, respectively. The average recoveries (n=6) were 101.5%, 101.6%, 98.61% and 99.77%, with RSDs of 1.7%, 1.5%, 1.42% and 0.79%, respectively. CONCLUSION: The method is simple, rapid, accurate, with low toxicity, and can be used to control the quality of Qingkailing dropping pills more effectively and comprehensively.

16.
Article in Chinese | WPRIM | ID: wpr-793036

ABSTRACT

OBJECTIVE@#To compare the clinical effect between wheat-grain moxibustion at Yinbai (SP 1) and oral administration of dydrogesterone tablet for menstrual period prolongation after down-regulation treatment of in vitro fertilization embryo transfer (IVF-ET).@*METHODS@#A total of 54 patients with prolonged menstrual period after down-regulation treatment of IVF-ET were randomly divided into an observation group and a control group, 27 cases in each one. In the observation group, when the menstrual period delayed more than 7 days, the wheat-grain moxibustion at Yinbai (SP 1) was performed, once a day, with an interval of 1 day between two 3-day treatments; when the menstrual blood was cleaned, the ovulation was continued and the eggs were taken. In the control group, when the menstrual period delayed more than 7 days, the oral administration of dydrogesterone tablet was provided, 10 mg each time, twice a day; when the menstrual blood was cleaned, the ovulation was continued and the eggs were taken. The number of days for menstrual blood to be cleaned, the area change of uterine cavity hemorrhage, the morphology of endometrium, the blood supply of endometrium, the number of oocytes obtained, the grade of frozen embryo and the clinical effect were observed between the two groups after treatment.@*RESULTS@#Compared with the control group, the number of days for menstrual blood to be cleaned was shorter in the observation group after treatment (0.05). The cured rate in the observation group was 100.0% (27/27), higher than 33.3% (9/27) in the control group (<0.05).@*CONCLUSION@#The wheat-grain moxibustion at Yinbai (SP 1) could more effectively treat prolonged menstrual period after IVF-ET down-regulation treatment, which is beneficial to the preparation of the endometrium, and has no effect on the oocyte collection and embryo culture.

17.
Article in Chinese | WPRIM | ID: wpr-828727

ABSTRACT

OBJECTIVE@#To study the clinical features of severe type 7 adenovirus pneumonia in children.@*METHODS@#A retrospective analysis was performed for the clinical data of children who were diagnosed with severe type 7 adenovirus pneumonia from February to June, 2019.@*RESULTS@#Among the 45 children, the male/female ratio was 3:2 and the median age was 14 months. All children had repeated fever, cough, and pulmonary moist rales, and the mean duration of fever was 14±4 days. The median time from fever to dyspnea was 8 days, and the time from fever to mechanical ventilation was 11.6±2.5 d. There was no significant increase in white blood cell count, with neutrophils as the main type. There were slight reductions in hemoglobin and albumin, while platelet and fibrinogen remained normal. There were increases in aspartate aminotransferase, lactate dehydrogenase, procalcitonin, and C-reaction protein. The detection rate of mixed pathogens was 84%. Effusion in both lungs was the major change on chest imaging (64%). Bronchoscopic manifestations were endobronchitis, tracheomalacia, and plastic bronchitis. The incidence rate of respiratory complications was 100%, and extrapulmonary complications mainly involved the circulatory system (47%), digestive system (36%), and nervous system (31%). Among the 45 children, 16 were administered with 400 mg/kg intravenous immunoglobulin (IVIG) daily for 5 days, with a mean duration of fever of 16±5 days, and 29 were administered with 1 g/kg IVIG daily for 2 days, with a mean duration of fever of 13±4 days; there was a significant difference in the mean duration of fever between the two groups (P=0.046). The overall mortality rate was 11%.@*CONCLUSIONS@#Severe type 7 adenovirus pneumonia in children has severe conditions, with a high incidence rate of complications and a high mortality rate, so it should be diagnosed and treated as early as possible.


Subject(s)
Adenoviridae , Bronchitis , Female , Fever , Humans , Infant , Male , Pneumonia, Viral , Retrospective Studies
18.
Article in Chinese | WPRIM | ID: wpr-777442

ABSTRACT

To obtain biocontrol fungus for Alternaria panax,the antifungal effects of one strain of endophytic fungi isolated from leaves of healthy ginseng were screened and evaluated by using dual-culture method,and the taxonomic assignment of the screened strain was identified based on the morphological characters and ITS sequence analysis. The results of dual-culture showed that one of the endophytes marked as FS-01 had good antifungal effects and the inhibitory rates of FS-01 strain to A. panax was( 60. 21±0. 12) %.The hyphae junction of the both strains,A. panax dissolved,broke and winded,while the hyphae of FS-01 strain remained normal. The inhibitory rates of non-sterilized FS-01 strain fermentation liqud was( 13. 94±0. 21) %. Strain FS-01 identified as Chaetomium globosum.


Subject(s)
Alternaria , Virulence , Antibiosis , Chaetomium , Classification , Endophytes , Fungicides, Industrial , Panax , Microbiology , Plant Diseases
19.
Article in English | WPRIM | ID: wpr-773400

ABSTRACT

OBJECTIVE@#To assess the activities of biapenem against multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis.@*METHODS@#Biapenem/clavulanate (BP/CL) was evaluated for in vitro activity against Mycobacterium tuberculosis (Mtb) multidrug-resistant (MDR) isolates, extensively drug-resistant (XDR) isolates, and the H37RV strain. BP/CL activity against the H37Rv strain was assessed in liquid cultures, in macrophages, and in mice..@*RESULTS@#BP/CL exhibited activity against MDR and XDR Mtb isolates in liquid cultures. BP/CL treatment significantly reduced the number of colony forming units (CFU) of Mtb within macrophages compared with control untreated infected macrophages. Notably, BP/CL synergized in pairwise combinations with protionamide, aminosalicylate, and capreomycin to achieve a fractional inhibitory concentration for each pairing of 0.375 in vitro. In a mouse tuberculosis infection model, the efficacy of a cocktail of levofloxacin + pyrazinamide + protionamide + aminosalicylate against Mtb increased when the cocktail was combined with BP/CL, achieving efficacy similar to that of the positive control treatment (isoniazid + rifampin + pyrazinamide) after 2 months of treatment.@*CONCLUSION@#BP/CL may provide a new option to clinically treat MDR tuberculosis.


Subject(s)
Animals , Anti-Infective Agents , Pharmacology , Therapeutic Uses , Cell Line , Drug Evaluation, Preclinical , Macrophages , Mice , Mycobacterium tuberculosis , Thienamycins , Pharmacology , Therapeutic Uses , Tuberculosis, Multidrug-Resistant , Drug Therapy
20.
Article in Chinese | WPRIM | ID: wpr-773142

ABSTRACT

@#Alpinia oxyphylla is mainly produced in Hainan,and also one of the four famous traditional Chinese medicines in South China with increasing importance in traditional Chinese medicine industry. Field surveys and literatures show that A. oxyphylla has widely used as a medicinal and edible plant,it is an important raw material for many Chinese patent medicines,health products and food,with a long history of artificial cultivation and application. The future development is prospected on health market. But A. oxyphylla industry has faced a lot of problems,including unreasonable planting layout,lack of good varieties,imperfect seed breeding system,low level of standardization,inconsistent quality of medicinal materials,low level of industry,and so on. The suggestions for sustainable development are listed below.First,it is essential to strengthen the research on the basis and application technology of A. oxyphylla,speed up the selection and breeding of improved varieties,and popularize standardized cultivation techniques. Secondly,it is important to strengthen the research on quality standards,improve the quality evaluation system of medicinal materials. Thirdly,it is necessary to take full advantage of the functional components to develop functional products with Hainan characteristics,find out the unique product characteristics of A. oxyphylla,build a famous brand and improve the product competitiveness in the market. It is also important to strengthen policy support and industrial supervision,promote the healthy and rapid development of A. oxyphylla industry.


Subject(s)
Alpinia , Chemistry , China , Drugs, Chinese Herbal , Pharmacology , Medicine, Chinese Traditional , Plant Breeding , Plants, Medicinal , Chemistry , Seeds
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