ABSTRACT
By applying "homeostasis" to the study of the meridian and collateral system， the concept of meridian and collateral homeostasis has been proposed which refers to a balanced and stable state of meridian and collateral system， and plays an important role in maintaining body health and can provide a reference for the diagnosis and treatment of diseases. Phenomics realizes the cross-scale correlation from micro-phenotypic data， such as genome， proteome， and metabolome， to macro-phenotypic data， such as physiological state， behavioral activities， and external manifestations. From the perspective of phenomics， this paper proposes a meridian and collateral homeostasis dynamic mapping model of "macroscopic signs and microscopic expression". This model combines macro signs such as the four examinations of traditional Chinese medicine （TCM）， biophysical indicators of acupoints， and micro expression information such as genes， proteins， and metabolism， and systematically investigates the relationship between meridian and collateral homeostasis and health and disease， thereby providing ideas and references for the identification of pre-disease states as well as precise diagnosis and treatment in TCM.
ABSTRACT
Objective:To identify Huanglianjiedu Decoction preparation (Rhizoma Coptidis, Radix Scutellariae, Cortex Phellodendri, Fructus Gardeniae) made from different technics and Japanese Kampo with fingerprint chromatographic. Methods: The HPLC method was used, chromatography conditions were C 18V column, binary solvent-delivery gradient elution, diode-array detector, internal standard (paeoniflorin) were taken as the criteria for quality control. Results: The result showed that 15～16 characteristic peaks were common, but the new technic preparation and EtOH extract showed a specific characteristic peak. And significant deviation has been found from peak-height ratio of characteristic peaks to internal standard in various preparations, which were made from different technics or from different materials. Conclusion: The good reproducibility and stability are showed in experiment results. The HPLC fingerprint can be used for identifying this traditional Chinese medicine preparations which were made from different technics or from different materials.
ABSTRACT
Objective:To establish a HPLC fingerprint determination which could separate the main principles of alkaloids, flavonoids and iridoids in Huanglianjiedu Decoction. Methods: The HPLC method was used and chromatography conditions were C 18V column, binary or ternary solvent-delivery gradient elution and diode-array detector. Results: A HPLC fingerprint determination method was established, which could separate main principles of the three effective groups of Huanglianjiedu Decoction. Conclusion: The ingredients of traditional Chinese medicine consist of several effective groups with different chemicals and physical properties, compared with the plant standard extracts, further studies are required for establishing HPLC fingerprint.