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1.
Article in Chinese | WPRIM | ID: wpr-921777

ABSTRACT

A HPLC method was established for simultaneous determination of two organic acids(chlorogenic acid and ferulic acid) and five phthalides(senkyunolide I, senkyunolide H, senkyunolide A, ligustilide, and butylidenephthalide) in Angelicae Sinensis Radix and its processed products to clarify the underlying material transferring rules. The analysis was performed on a Welch Ultimate C_8 column(4.6 mm×250 mm, 5 μm) with acetonitrile(A)-0.085% phosphoric acid water(B) as the mobile phase in a gradient elution mode at the flow rate of 1.1 mL·min~(-1), the column temperature of 25 ℃, the detection wavelength of 280 nm, and the injection volume of 10 μL. Under these conditions, the content of the above-mentioned seven components was analyzed in 15 batches of Angelicae Sinensis Radix and its processed products, and the transfer rate of each compound was calculated. As a result, in the processed products, the average content of chlorogenic acid was slightly decreased and that of ferulic acid was equivalent to the medicinal materials. The content of senkyunolide I, senkyunolide H, senkyunolide A, and butylidenephthalide showed an increasing trend in the processed products as compared with the medicinal materials. The mass fraction of ligustilide in the medicinal materials was above 0.7%(0.94% on average), meeting the requirement of 0.6% in the Hong Kong Chinese Materia Medica Standards, but was 0.47% on average in the processed products, which was decreased by 50% approximately. Further investigation showed that the content of ligustilide in freshly made processed products of Angelicae Sinensis Radix did not change significantly compared with that in the medicinal materials, indicating that the loss of ligustilide in the processed products mainly occurred in the storage. Therefore, Angelicae Sinensis Radix is suitable for storing in the form of medicinal materials and the freshly made processed products should be used except for special cases. Additionally, it is recommended to control the content of volatile oils or ligustilide in medicinal materials and processed products of Angelicae Sinensis Radix to ensure its effectiveness in clinical medication.


Subject(s)
Angelica sinensis , Chlorogenic Acid , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Plant Roots
2.
Article in Chinese | WPRIM | ID: wpr-906451

ABSTRACT

Objective:To observe the effects of Da Chaihutang on Cyclic adenosine monophosphate (cAMP)-response element binding protein (CREB)/peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1<italic>α</italic>) pathway in nutritionally obese rats and the protective mechanism on liver mitochondria. Method:A total of 120 8-week-old male SD rats were randomly divided into a control group (<italic>n</italic>=20) and an experimental group (<italic>n</italic>=100). The rats in the control group were fed on a normal diet, while those in the experimental group were administered with a high-fat feed. Successfully modeled rats were randomly divided into a model group, a positive drug (metformin) group, and low-, medium- and high-dose Da Chaihutang groups (4.25, 8.5, and 17 g∙kg<sup>-1</sup>, respectively), with 20 rats in each group. After treatment with Da Chaihutang, the body weight, Lee's index, liver mitochondrial membrane potential and mitochondrial ultrastructure, PGC-1<italic>α </italic>expression and CREB phosphorylation of each group were measured and compared. Result:Compared with the control group, the model group showed increased body weight and Lee's index (<italic>P</italic><0.01), whereas decreased mitochondrial membrane potential, PGC-1<italic>α</italic> expression, and CREB phosphorylation level (<italic>P</italic><0.01). As compared with the model group, Da Chaihutang significantly reduced the body weight and Lee's index of obese rats (<italic>P</italic><0.05, <italic>P</italic><0.01), enhanced liver mitochondrial membrane potential (<italic>P</italic><0.05, <italic>P</italic><0.01) to protect the integrity of mitochondrial structure, up-regulated PGC-1<italic>α</italic> expression and promoted CREB phosphorylation (<italic>P</italic><0.05, <italic>P</italic><0.01). Conclusion:Da Chaihutang protects the structure and function of mitochondria and inhibits weight gain in obese rats by activating the CREB/PGC-1<italic>α</italic> pathway.

3.
Article in Chinese | WPRIM | ID: wpr-906073

ABSTRACT

Nardostachyos Radix et Rhizoma is the dry root and rhizome of Nardostachys jatamansi (Valerianaceae) with a long medical history and a broad range of application, which is effective in regulating Qi, relieving pain, resolving depression, and enlivening spleen, as well as dispelling dampness and relieving swelling by external application. It can be used for the treatment of abdominal distension, loss of appetite, and vomiting. Besides, it can also relieve toothache and treat dermatophytosis and pyogenic infection by external use. Moreover, it serves as a common medicinal material in ancient Ayurveda and Unani medical systems in India and also as an ingredient in spices, foods, and cosmetics. Modern pharmacological studies have shown that Nardostachyos Radix et Rhizoma possesses multiple pharmacological activities, such as sedation, anti-epilepsy, anti-convulsion, anti-depression, anti-arrhythmia, anti-malaria, anti-inflammation, anti-bacteria, anti-oxidation, and blood sugar metabolism improvement due to its multiple compounds contained, including terpenes, flavonoids, coumarins, and lignans. The main active components are sesquiterpenoids represented by nardosinone. The pharmacological activities, chemical compositions, and clinical applications of Nardostachyos Radix et Rhizoma have been investigated, but the research on resources, distribution, quality control, cultivation status, and applications are rarely reported. As an important genuine medicinal material from the Qinghai-Tibet plateau, Nardostachyos Radix et Rhizoma is obtained mainly from wild N. jatamansi. Accordingly, the conservation and sustainable utilization of N. jatamansi have attracted much attention all around the world. Based on the resource survey, cultivation research, and relevant literature available, the present study reviewed resources, geographical distribution, chemical compositions, pharmacological activities, quality control, cultivation, and applications of N. jatamansi, aiming to provide references for the conservation and development of N. jatamansi.

4.
Article in Chinese | WPRIM | ID: wpr-873560

ABSTRACT

Objective To explore the impact of the two-child policy on the fertility level of residents in Yangpu District. Methods Birth registry data were collected from the residents of Yangpu District in Shanghai during January 2015 to December 2018 for the analysis of birth characteristics and its variation after the two-child policy implementation. Results The total fertility rate was higher than 1 only in 2016 that was 1.04, whereas it was about 0.8 in other three years. The number of the second child increased which was inspired by the policy. However, the increment in the number of second child was far less than the decrease in the first child. From 2015 through 2018, the proportion of second children in the total births with local household registration accounted for 18.84%, 20.03%, 26.29% and 24.35%, respectively. From January 2015 to August 2016, the proportion of second children was on the rise slowly, in which the annual percent change(APC)value was 0.34%(P > 0.05). From September 2016 to May 2017, the proportion of second children had shown an obvious increase, and the APC value was 3.97%(P < 0.05). From June 2017 to December 2018, the proportion of second children was on a downward trend, in which the APC value was -0.97%(P < 0.05). Conclusion Favorable fertility policy may not reverse the fertility level in Yangpu District, which remains at a very low level.

5.
Article in Chinese | WPRIM | ID: wpr-792969

ABSTRACT

Hypoxic-ischemic encephalopathy (HIE) is the leading cause of neonatal death and neurodevelopmental disorders in infants. Part of patients have different degrees of neurological sequelae, such as cerebral palsy, cognitive and motor function development disorders. Hypoxia-ischemia may activate JAK2/STAT3 signaling pathway, which leads to the microglia activation and neuroinflammation. Down-Regulating JAK2/STAT3 signaling pathway can inhibit microglia activation and regulate the inflammatory injury of nervous system. At present, the treatment of hypoxic ischemic encephalopathy is limited, so the study of regulatory mechanism about microglia activation has important value for the treatment of hypoxic-ischemic encephalopathy. This paper summarizes the role of JAK2/STAT3 signaling pathway in microglia activation and analyzes the relationship between them, in order to provide new ideas and strategies for treatment on hypoxic-ischemic encephalopathy.

6.
Chinese Medical Journal ; (24): 1057-1065, 2020.
Article in English | WPRIM | ID: wpr-827662

ABSTRACT

BACKGROUND@#Preeclampsia (PE) is a serious complication that affects maternal and perinatal outcomes. However, the mechanisms have not been fully explained. This study was designed to analyze longitudinal gut microbiota alterations in pregnant women with and without PE in the second (T2) and third trimesters (T3).@*METHODS@#In this nested case-control study, which was conducted at Nanjing Maternity and Child Health Care Hospital, fecal samples from 25 PE patients (25 fecal samples obtained in T2 and 15 fecal samples obtained in T3) and 25 matched healthy controls (25 fecal samples obtained in T2 and 22 fecal samples obtained in T3) were collected, and the microbiota were analyzed using 16S rRNA gene sequencing. The diversity and composition of the microbiota of PE cases and controls were compared.@*RESULTS@#No significant differences in diversity were found between the PE and control groups (P > 0.05). In the control group, from T2 to T3, the relative abundances of Proteobacteria (median [Q1, Q3]: 2.25% [1.24%, 3.30%] vs. 0.64% [0.20%, 1.20%], Z = -3.880, P < 0.05), and Tenericutes (median [Q1, Q3]: 0.12% [0.03%, 3.10%] vs. 0.03% [0.02%, 0.17%], Z = -2.369, P < 0.05) decreased significantly. In the PE group, the relative abundance of Bacteroidetes in T2 was lower than in T3 (median [Q1, Q3]: 18.16% [12.99%, 30.46%] vs. 31.09% [19.89%, 46.06%], Z = -2.417, P < 0.05). In T2, the relative abundances of mircrobiota showed no significant differences between the PE group and the control group. However, in T3, the relative abundance of Firmicutes was significantly lower in the PE group than in the control group (mean ± standard deviation: 60.62% ± 15.17% vs. 75.57% ± 11.53%, t = -3.405, P < 0.05). The relative abundances of Bacteroidetes, Proteobacteria, and Enterobacteriaceae were significantly higher in the PE group than in the control group (median [Q1, Q3]: 31.09% [19.89%, 46.06%] vs. 18.24% [12.90%, 32.04%], Z = -2.537, P < 0.05; 1.52% [1.05%, 2.61%] vs. 0.64% [0.20%, 1.20%], Z = -3.310, P < 0.05; 0.75% [0.20%, 1.00%] vs. 0.01% [0.004%, 0.023%], Z = -4.152, P < 0.05). Linear discriminant analysis combined effect size measurements analysis showed that the relative abundances of the phylum Bacteroidetes, class Bacteroidia and order Bacteroidales were increased in the PE group, while those of the phylum Firmicutes, the class Clostridia, the order Clostridiales, and the genus unidentified Lachnospiraceae were decreased in the PE group; and these differences were identified as taxonomic biomarkers of PE in T3.@*CONCLUSION@#From T2 to T3, there was an obvious alteration in the gut microbiota. The gut microbiota of PE patients in T3 was significantly different from that of the control group.

7.
Article in Chinese | WPRIM | ID: wpr-776121

ABSTRACT

OBJECTIVE@#To compare computer measurement semi automatic quantitative detection software system with ultra thin layer pathological section to confirm the avascular necrosis percentage of the femoral head, and provide reference for clinical treatment options.@*METHODS@#From June 2012 to December 2013, the X-ray and MRI of 24 patients(24 hips on unilateral) were reviewed, who had avascular necrosis of the femoral head at late stage (stage III and IV) according to the ARCO international staging system, and performed by total hip arthroplasty. There were 15 males and 9 females, with an average age of (65.1±8.8) years old ranging 33 to 74 years old. Based on imaging system combined with computer aided technology(CAD) system and postoperative ultrathin slice, the volume of the area of femoral head necrosis were calculated. Then combining with the total volume of the femoral head was calculated by X-ray, the percentage of each femoral head necrosis area was calculated as a percentage of the whole ball head in both methods.@*RESULTS@#For 24 hips, the normal femoral head volume was (39.58±3.29) cm³ on average of X-ray film, the necrotic volume was (20.00±3.04) cm³ on average of MRI by the calculation of computer. The necrotic volume occupying in the volume of the normal femoral head was (42.92±6.09)% on average ranged. The necrotic lesion size was larger than 40% in 16 hips, 30% to 40% in 8 hips, 1 ess than 30% in 0 hip. Under the pathological section, the necrosis volume was (19.89±3.17) cm³, the necrotic volume occupying in the volume of the normal femoral head was (40.33±6.36)%. The necrotic lesion size was larger than 40% in 12 hips, 30% to 40% in 11 hips, 1 ess than 30% in 1 hip. In computer and general measurement, the two entire femoral head volume difference was (0.113±0.466) cm³, there was no significant difference using these measurements(=-1.186, =0.248). After the linear correlation statistics analysis, there was a positive correlation relationship in necrotic volume between computer software and pathology measurement(γ=0.980, =0.000).@*CONCLUSIONS@#The severity of the necrotic femoral head is closely related to the percentage of necrotic area. When the ratio of necrotic lesions and the whole femoral head is greater than 30%, the patients' clinical symptoms tend to aggravate, the probability of total hip arthroplasty increased significantly. It have great significances in femoral head necrosis installment, judge the prognosis, guide the selection of treatment and monitoring curative effect.


Subject(s)
Adult , Aged , Arthroplasty, Replacement, Hip , Female , Femur , Femur Head , Femur Head Necrosis , Diagnostic Imaging , Follow-Up Studies , Hip , Humans , Male , Middle Aged
8.
Article in Chinese | WPRIM | ID: wpr-773186

ABSTRACT

Due to lack of reference substances,the content of triterpenoid saponins in traditional Chinese medicines is usually characterized by colorimetric determination of total saponins. However,the specificity of colorimetric method is poor,and the determination result is not accurate enough. So,in this paper,the content determination method of total triterpenoid saponins was studied by taking Akebiae Caulis saponins as an example. The contents of three main saponin aglycones,including arjunolic acid,hederagenin and oleanolic acid,were determined by HPLC method. Referring to the content determination method of total flavonol glycosides in Ginkgo biloba leaves in the 2015 edition of Chinese Pharmacopoeia,the content of Akebiae Caulis saponins was obtained by multiplying the total content of the three above-mentioned aglycones with conversion coefficient. LC-MS/MS analysis results showed that mutongsaponin C and aponin PJIwere the two main triterpene saponins in Akebiae Caulis,and they shared the same molecular formula. So,the average value of the ratios of the molecular weight between mutongsaponin C and the three aglycones was defined as the conversion coefficient.The three aglycones were separated on an ACE Excel 3 C18-AR column( 4. 6 mm×150 mm,3 μm),and methanol-water( containing0. 04% glacial acetic acid and 0. 02% triethylamine) was used as mobile phase with gradient elution. The detection wavelength was set at 210 nm,and the flow rate was 0. 5 m L·min-1. The results showed that there was a good linearity among the ranges of 1. 053-16. 84,0. 200-3. 200 and 1. 515-24. 24 μg for arjunolic acid,hederagenin and oleanolic acid,respectively. Their average recoveries were97. 90%,97. 50% and 100. 5%,with RSD of 2. 0%,2. 9% and 2. 9%,respectively. The results of methodological investigation met the requirements of content determination. The conversion coefficient was 2. 31. This method is simple and reliable,and can be used for the determination of total triterpenoid saponins in Akebiae Caulis. The assay strategy can be used for the determination of total triterpenoid saponins in other traditional Chinese medicines.


Subject(s)
Chromatography, High Pressure Liquid , Chromatography, Liquid , Drugs, Chinese Herbal , Medicine, Chinese Traditional , Phytochemicals , Ranunculales , Chemistry , Saponins , Tandem Mass Spectrometry , Triterpenes
9.
Article in Chinese | WPRIM | ID: wpr-773151

ABSTRACT

To search for the active diuretic fractions of Clematidis Armandii Caulis( CAC) and determine its main active chemical components by using liquid chromatography-mass spectrometry( LC-MS) and diuretic activity evaluation. CAC 75% ethanol extracts and extracts from different polar solvents were orally administered to saline-loaded rats at different doses. 6 h urinary volume,p H and contents of electrolyte Na+,K+and Cl-were measured. The chemical components of the active fractions were separated and identified by ultra performance liquid chromatography-electrospray ionization-quadrupole time of flight-mass spectrometry( UPLC-ESI-Q-TOF-MS/MS) method. As compared with the control group,the urine volume was increased by 44%( P< 0. 01) and 34%( P < 0. 05) in CAC75% ethanol extract 57. 74 and 28. 8 mg·kg-1 groups respectively; the Na+excretion was increased by 52%( P< 0. 01) and 45%( P<0. 05),respectively; while the Cl-excretion was increased by 101%( P<0. 01) and 85%( P<0. 05),respectively. The urine volume,Na+excretion and Cl-excretion were increased by 50%( P< 0. 01),58%( P< 0. 05),and 65%( P< 0. 05) respectively in petroleum ether extract 70. 98 mg·kg-1 group as compared with the control group. While for the n-butanol extract 194. 18 mg·kg-1 group,the urine volume,Na+and Cl-excretion were increased by 42%( P<0. 01),41%( P<0. 05) and 97%( P<0. 01),respectively. The diuretic activity of other fractions was not obvious. There was no statistical difference in K+excretion in all groups. The results of LC-MS analysis showed that six compounds,including two sterols,one chromogen and three fatty acids,were identified from petroleum ether extract.Fourteen compounds,including six triterpenoid saponins,six lignin glycosides,one sterol glycoside and one phenolic glycoside,were identified from the n-butanol extract. All the results suggested that the ethanol extract of CAC had remarkable diuretic activity and its main effective components included sterol,triterpenoid saponin and lignin glycosides.


Subject(s)
Animals , Ascomycota , Chemistry , Diuretics , Pharmacology , Materia Medica , Pharmacology , Rats , Solvents , Spectrometry, Mass, Electrospray Ionization , Tandem Mass Spectrometry
10.
Article in Chinese | WPRIM | ID: wpr-773828

ABSTRACT

OBJECTIVE@#To explore the effect of selective lumbosacral posterior rhizotomy(SPR) on lower limb spasticity and gross motor function in patients with cerebral palsy.@*METHODS@#From January 2018 to October 2018, 47 patients with cerebral palsy were treated with rehabilitation alone and SPR combined with rehabilitation. According to whether SPR was performed, the patients were divided into group A and group B. Group A was treated with rehabilitation combined with SPR at lumbosacral level, and group B was treated with rehabilitation alone. There were 23 cases in group A, including 15 males and 8 females, with an average age of (7.30±3.25) years old; 24 cases in group B, 13 males and 11 females, with an average age of (7.00±3.09) years old. Forty-seven patients were assessed with modified Ashworth(MAS) and Gross Motor Function Scale(GMFM-88 items) before and after treatment. The changes of MAS and GMFM-88 scores before and after treatment were compared to evaluate the degree of spasm and the improvement of gross motor function in the two groups.@*RESULTS@#All 47 patients were followed up. At 6 months after treatment, the MAS classification of the two groups was significantly improved(<0.05), and the improvement of group A was more obvious than that of group B(<0.05). Six months after treatment, the D, E and total scores of GMFM-88 between two groups were significantly improved compared with those before operation(<0.05). The improvement of D and total scores in group A was more obvious than that in group B. There was no significant difference in the improvement of area E between two groups.@*CONCLUSIONS@#Selective posterior rhizotomy combined with rehabilitation can significantly improve the spastic state and gross motor function of lower limbs in children with cerebral palsy, and can effectively promote the reconstruction and recovery of motor function of lower limbs in children with cerebral palsy.


Subject(s)
Cerebral Palsy , General Surgery , Child , Child, Preschool , Female , Humans , Lower Extremity , Male , Movement , Muscle Spasticity , Rhizotomy
11.
Article in Chinese | WPRIM | ID: wpr-802096

ABSTRACT

Objective: To study the accumulation of mercury in liver, kidney and brain of rats and its toxicity on liver and kidney after 4 weeks of administration of different doses of Zhuhong ointment, in order to provide data reference for the safe clinical use of Zhuhong ointment. Method: Forty-four Sprague-Dawley (SD) rats were randomly divided into 4 groups:control group, normal-dose group (1.875 mg·kg-1), medium-dose group (37.5 mg·kg-1), and high-dose group (75 mg·kg-1). After transdermal administration for consecutive 4 weeks, the mercury content in the urine, blood, liver, kidney and brain of the rats was measured. In addition, serum alanine aminotransferase (ALT), serum aspartate aminotransferase (AST), serum urea nitrogen (BUN), and serum creatinine (SCr), urine β2-miSCroglobulin (β2-BMG) and urine N-acetyl-beta-D glucosidase (NAG) contents were measured, and pathological morphology changes of liver and kidney were observed. Result:Compared with the control group, the levels of blood mercury and urine mercury in Zhuhong ointment groups showed significant increases after administration for 4 weeks(PPPPPPConclusion:Zhuhong ointment is not toxic at the normal dose, but long-term use can lead to the accumulation of mercury in liver, kidney and bra, which causes liver and kidney toxicity. This study did not find a more sensitive indicator of liver and kidney toxicity than liver and kidney pathology. However, because the rising levels of urinary mercury and blood mercury may predict toxicity, the relationship between mercury exposure and toxicity could be further studied. This study provides a reference for the rational use and toxicity monitoring of Zhuhong ointment.

12.
Article in Chinese | WPRIM | ID: wpr-300361

ABSTRACT

<p><b>OBJECTIVE</b>To study the difference in expression of TOPK/PBK in lymph nodes between children with malignant lymphoma and those with reactive lymphoid hyperplasia.</p><p><b>METHODS</b>Eighty children with malignant lymphoma and twenty children with reactive lymphoid hyperplasia were enrolled as subjects. Immunohistochemistry was used to determine the expression of TOPK/PBK in all the subjects. The expression of TOPK/PBK was compared between the two groups.</p><p><b>RESULTS</b>The TOPK/PBK-positivity rate was significantly higher in children with malignant lymphoma than in those with reactive lymphoid hyperplasia (P<0.05). There was no significant difference in the TOPK/PBK-positivity rate between the children with Hodgkin's lymphoma and non-Hodgkin's lymphoma (NHL). There were significant differences in the TOPK/PBK-positivity rate among children with different pathological types of NHL (P<0.05): the children with lymphoblastic lymphoma showed the highest TOPK/PBK-positivity rate and those with mature B-cell lymphoma and mature T/NK-cell lymphoma had a similar TOPK/PBK-positivity rate.</p><p><b>CONCLUSIONS</b>The expression of TOPK/PBK is up-regulated in the lymph nodes of children with malignant lymphoma. The expression level of TOPK/PBK may be related to the pathological type of NHL.</p>


Subject(s)
Adolescent , Child , Child, Preschool , Humans , Infant , Infant, Newborn , Lymph Nodes , Lymphoma , Mitogen-Activated Protein Kinase Kinases , Pseudolymphoma
13.
Article in Chinese | WPRIM | ID: wpr-699610

ABSTRACT

Objective To investigate the protective effects of tribulus terrestris L (TTL) against light-induced photoreceptor degeneration and its underlying mechanisms.Methods BALB/c mice were randomly divided into normal control group,light exposure group and TTL administration group,with 12 mice in each group,and then exposed to light at the intensity of 10,000 lux for 30 min for the establishment of a retinal damage model of BALB/C mice.And 100 μL TTL decoction was intraperitoneally administered into mice of TTL administration group 30 min prior to illumination.Saline vehicle was administrated into the mice of normal control group and light exposure group.Next,intraperitoneal injection of dihydroethidium (DHE) was performed 22 h after illumination,and the eyes were enucleated 2 h later and subjected to cryosectioning for microscopic detection of the in situ retinal oxidative stress.Then,retinas were dissected 6 and 24 h after illumination,which was followed by total RNA extraction,reverse transcription and RT-PCR to assess the expression level of proinflammatory cytokines.Meanwhile,the expression levels of interleukin-β (IL-1 β),chemokine (Ccl2),cyclooxygenase-2 (COX-2),tumor necrosis factor-α (TNF-α),cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) mRNA were determined.Results Prominent oxidative stress was observed in retinal pigment epithelial cells and photoreceptor cells in the light exposure group when compared with the normal control group and TTL administration group,with significant difference (both P < 0.001).Moreover,results of RT-PCR revealed that the expression of IL-1β,Ccl2,COX-2,TNF-α,ICAM-1 and VCAM-1 mRNA was significantly elevated as a result of light exposure compared to those from vehicle-treated normal controls with a significant difference (all P < 0.01).TTL treatment resulted in significantly decreased expression of IL-1β,Ccl2,COX-2,TNF-α,ICAM-1 and VCAM-1 mRNA compared to those from light-exposed vehicle-treated controls with significant differences (all P <0.01).Conclusion In the retinal degeneration model,TTL protects the photoreceptor cells against fight-induced degeneration in part through suppressing light-induced retinal oxidative stress and inflammatory responses.

14.
Article in Chinese | WPRIM | ID: wpr-695675

ABSTRACT

Objective·To compare the differences in cardiac function and neurological function between asphyxia and ventricular fibrillation (VF)induced cardiac arrest rat model.Methods·Twenty healthy adult male SD rats were randomly divided into VF group (n=8),asphyxial group (n=8)and sham group (n=4).Cardiac arrest models were established in VF group and asphyxial group by VF and asphyxia respectively.All animals were observed for 24 h and advanced life support was offered for the first 1 h after resuscitation.During the 24 h,ejection fraction (EF) and cardiac output (CO) were measured with the help of cardiac ultrasonography at 1,3,5 and 6 h post resuscitation.Electrocardiographic changes,24 h survival analysis and neurological deficit score (NDS) were also recorded and analyzed at 6,12,18 and 24 h post resuscitation.Results·Both EF and CO decreased dramatically after resuscitation compared with sham group at the same time point (P=0.000).At 1 h post resuscitation,the CO decreased from (98.84±4.86)mL/min to (59.17±22.99) mL/min in VF group and from (99.86±10.34) mL/min to (46,02±22.32) mL/min in asphyxial group,but there was no difference between the two groups (P=0.792).At 3,5 and 6 h post resuscitation,the CO in VF group was higher than that in asphyxial group (P=0.041,P=0.007,P=0.020).At 1 h post resuscitation,the EF decreased from (82.67±6.21)% to (70.23±13.24)% in VF group and from (83.24±3.01)% to (65.46±13.11)%in asphyxial group,but no difference was observed between the two groups (P=0.877).Then a recovery tendency was observed in both groups,but more obvious in VF group at 3 and 5 h post resuscitation (P=0.031,P=0.024).No difference was found between the two groups in survival rate during 24 h and the NDS after resuscitation,although the neurological function was greatly impaired.Conclusion·VF and asphyxia are most commonly used methods to induce cardiac arrest,but these models may differ in cardiac function post resuscitation.Researchers need to choose appropriate models according to their study objectives.

15.
Journal of Practical Radiology ; (12): 1937-1940,1948, 2018.
Article in Chinese | WPRIM | ID: wpr-733398

ABSTRACT

Objective To quantitatively assess the liver fat content by Nav IDEAL-IQ and compare with conventional breath-hold IDEAL-IQ and magnetic resonance spectroscopy (MRS).Methods Twenty volunteers,twenty fatty liver patients and twenty patients with other liver diseases were enrolled in this study.IDEAL-IQ,Nav IDEAL-IQ and MRS imaging were performed and fat fraction (FF) were measured respectively.The results were compared by t-test to explore whether there were differences between these groups. Spearman correlation analysis was also used to test the correlation.Results No significant differences in the FF measured by IDEAL-IQ,Nav IDEAL-IQ and MRS in normal volunteers [(4.09±0.82)%,(3.94±0.83)%,(3.92±0.85)%],fatty liver [(15.5±6.1)%,(11.8±6.5)%,(1 2 .4 ± 7 .2 )% ] and other liver lesions patients [(4.20±0.84)%,(4.00±0.94)%,(3.97±0.96)%]were found between these groups (P>0.05). There was a significant positive correlation between Nav IDEAL-IQ and MRS,and the correlation coefficients of these groups were 0.959,0.786 and 0.945,respectively.Conclusion The results of Nav IDEAL-IQ of fat quantification are consistent with MRS results and it increases the success rate of fat quantitative testing.In a word,it is a noninvasive,convenient and accurate way to measure fat fraction.

16.
Article in Chinese | WPRIM | ID: wpr-351397

ABSTRACT

A two-year-old girl was admitted due to repeated yellowing of the skin and sclera for 2 years and had no other specific symptoms or signs. The use of phenobarbital could relieve the symptoms of jaundice. Multiple examinations showed increased indirect bilirubin levels, and the results of aminotransferases and liver imaging were normal. There was no evidence of hemolysis. The analysis of UGT1A1 gene in her family found that this child had double homozygous mutation of c.211G>A(G71R) and c.1456T>G(Y486D), which had been reported as the pathogenic mutation for Gilbert syndrome. Her parents carried double heterozygous mutation of G71R and Y486D and had no symptom of jaundice. The child was diagnosed as having Gilbert syndrome. It is concluded that as for patients with unconjugated hyperbilirubinemia which cannot be explained by liver damage and hemolysis, their family history should be investigated in detail and gene analysis should be performed as early as possible, in order to identify congenital bilirubin metabolic disorders.


Subject(s)
Child, Preschool , Female , Gilbert Disease , Diagnosis , Glucuronosyltransferase , Genetics , Humans , Mutation , Sclera , Pathology , Skin , Pathology
17.
Journal of Preventive Medicine ; (12): 20-22,27, 2017.
Article in Chinese | WPRIM | ID: wpr-792578

ABSTRACT

Objective To investigate the inducing factors of diabetic ketosis(DK)in type 2 diabetes mellitus( T2DM) patients for more effective prevention. Methods A total of 545 T2DM patients with DK who received treatment in Zhejiang Provincial Diabetes Prevention and Treatment Center from June 2011 to June 2015 were enrolled,which contains 297 newly- diagnosed ketosis - prone diabetic(KPD)patients(KPD group)and 248 T2DM patients with DK(T2DM group),the etiological factors and clinical characteristics were analyzed retrospectively. Results The leading causes of DK were long term nonintervetion(71. 71% )and infection(15. 15% )in KPD group,while unadjusted therapeutic regimen(39. 91% )and break - off(22. 98% )and infection(20. 97% )were the leading cause in T2DM group. Compared with T2DM group, patients in KPD group were younger(P ﹤ 0. 01),and had lower BMI index(P ﹤ 0. 01),higher HbA1c level(P ﹤ 0. 01), more serious ketosis patients(urine ketone≥ ﹢ ﹢ ,P ﹤ 0. 05)and more DKA patients(P ﹤ 0. 05). Conclusion T2DM patients with ketosis could not be ignored,especially the KPD patients. More attention should be paid to diabetes health education,and screening of high - risk group and long term follow - up in patients with T2DM are effective means to reduce the incidence of DK in T2DM.

18.
Journal of Medical Informatics ; (12): 81-83,87, 2017.
Article in Chinese | WPRIM | ID: wpr-669155

ABSTRACT

The paper states the connotation,development status,development trend and resource composition of micro course,discusses how do the hospital libraries provide excellent resources for micro course teaching and carry out services including personalized information service and embedded teaching service by taking advantage of micro course under "micro" environment,and indicates that librarians shall strengthen the learning of new subjects and new technologies.

19.
Article in Chinese | WPRIM | ID: wpr-665379

ABSTRACT

Objective·To explore the difference between multi-artery-graft off-pump coronary artery bypass grafting (OPCABG) and single-artery-graft OPCABG on left main coronary artery or multivessel disease with propensity score matching. Methods·A total of 1578 patients with left main coronary artery or multivessel disease underwent isolated OPCABG were selected in Ruijin Hospital from January 2012 to September 2016. The propensity score methodology was used to obtain risk-adjusted outcome. Kaplan-Meier analysis was applied for estimation of freedom from major adverse cardiac and cerebrovascular events (MACCE) and readmission for heart disease. Independent predictor of MACCE were assessed by Cox regression analysis. Results·The average follow-up time was 28 months (7-55 months). There was no statistical difference in short-term clinical endpoints in hospital. In the follow-up results, multi-artery-graft OPCABG patients had statistical improvement in readmission for heart disease (2.7% vs 12.7%, P=0.023), CCS class (1.2±0.4 vs 1.4±0.6, P=0.020) and patency rate of grafts in 1 year after operation (95.8% vs 85.9%, P=0.025), compared with single-artery-graft OPCABG. There was no statistical difference in other endpoints. There was statistical improvement for multi-artery-graft OPCABG patients in freedom from readmission for heart disease (P=0.028). Female was an independent predictor of MACCE (95% CI 0.117-0.906, P=0.032). Conclusion·Multi-artery-graft OPCABG appears to be safe and with good patency of grafts and clinical outcomes in treating left main coronary artery or multivessel disease. The follow up of female patients should be paid more attention.

20.
Article in Chinese | WPRIM | ID: wpr-510130

ABSTRACT

In recent years, many versions of international standard terminologies on TCM have been established and enacted by different authoritative organizations worldwide. However, infantile tuina acupoints have never been included in them. The article illustrated the necessity of including infantile tuina acupoints in the international standard terminologies on TCM from the aspects of history, present state and specificity of these points. It also pointed out some problems of infantile tuina acupoints, such as different records of acupoints numbers, applications in the clinic and strategies applied in their English translation, and suggestions were provided accordingly, hoping that the infantile tuina acupoints can be included in the further revised versions of international standard terminologies on TCM.

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