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1.
Chinese Medical Journal ; (24): 1093-1100, 2021.
Article in English | WPRIM | ID: wpr-878150

ABSTRACT

BACKGROUND@#Although osteopontin (OPN) is expressed in the liver and pigment gallstones of patients with hepatolithiasis, its role in pigment gallstone formation remains unclear. This study aimed to explore the function of OPN in pigment gallstone formation.@*METHODS@#Rats were fed a chow diet (CD) or lithogenic diet (LD) for 10 consecutive weeks; blocking tests were then performed using an OPN antibody (OPN-Ab). Incidence of gallstones and levels of several bile components, OPN, tumor necrosis factor alpha (TNF-α), and cholesterol 7 alpha-hydroxylase (CYP7A1) were analyzed. To determine TNF-α expression in hepatic macrophages and both CYP7A1 and bile acid (BA) expression in liver cells, recombinant rat OPN and recombinant rat TNF-α were used to treat rat hepatic macrophages and rat liver cells, respectively. Chi-square or Fisher exact tests were used to analyze qualitative data, Student t-test or one-way analysis of variance were used to analyze qualitative data.@*RESULTS@#Incidence of gallstones was higher in LD-fed rats than in CD-fed rats (80% vs. 10%, P < 0.05). BA content significantly decreased in bile (t = -36.08, P < 0.01) and liver tissue (t = -16.16, P < 0.01) of LD-fed rats. Both hepatic OPN protein expression (t = 9.78, P < 0.01) and TNF-α level (t = 8.83, P < 0.01) distinctly increased in the LD group; what's more, CYP7A1 mRNA and protein levels (t = -12.35, P < 0.01) were markedly down-regulated in the LD group. Following OPN-Ab pretreatment, gallstone formation decreased (85% vs. 25%, χ2 = 14.55, P < 0.01), liver TNF-α expression (F = 20.36, P < 0.01) was down-regulated in the LD group, and CYP7A1 expression (F = 17.51, P < 0.01) was up-regulated. Through CD44 and integrin receptors, OPN promoted TNF-α production in macrophage (F = 1041, P < 0.01), which suppressed CYP7A1 expression (F = 48.08, P < 0.01) and reduced liver BA synthesis (F = 119.4, P < 0.01).@*CONCLUSIONS@#We provide novel evidence of OPN involvement in pigmented gallstone pathogenesis in rats.


Subject(s)
Animals , Diet/adverse effects , Gallstones/etiology , Lithiasis , Liver , Liver Diseases , Osteopontin/genetics , Rats
2.
Chinese Medical Journal ; (24): 109-113, 2012.
Article in English | WPRIM | ID: wpr-333532

ABSTRACT

<p><b>BACKGROUND</b>Patients with xanthogranulomatous cholecystitis sometimes exhibit imaging and intraoperative findings that are similar to those of advanced gallbladder cancer, thus these patients are easily misdiagnosed. The present study aimed to investigate the characteristics of xanthogranulomatous cholecystitis masquerading as gallbladder cancer that could potentially aid in the correct diagnosis of this condition.</p><p><b>METHODS</b>The clinical, serological, radiological and operative features of twelve patients with obviously wall-thickening or mass-forming xanthogranulomatous cholecystitis were retrospectively analyzed. Additionally, the patient preoperative features were compared to those of 36 patients with advanced gallbladder cancers.</p><p><b>RESULTS</b>Twelve patients with xanthogranulomatous cholecystitis exhibited one to three episodes of acute cholecystitis within 0.5 to 7 months prior to admission to the hospital. Five of these patients exhibited concomitant choledocholithiasis, whereas no concomitant choledocholithiasis was identified in patients with advanced gallbladder cancer. The incidence of abdominal pain (χ(2) = 6.588, P = 0.010), acute cholecystitis (χ(2) = 29.176, P = 0.000), acute cholangitis (χ(2) = 6.349, P = 0.012), choledocholithiasis (χ(2) = 16.744, P = 0.000), carcinoembryonic antigen test (P = 0.007), CA125 (P = 0.001), and diffuse gallbladder wall thickening (χ(2) = 6.031, P = 0.014), continued mucosal line (χ(2) = 15.745, P = 0.000), homogeneous enhancement of mucosal line (χ(2) = 19.947, P = 0.000), submucosal hypoattenuated nodules or band (χ(2) = 18.607, P = 0.000) in computed tomography demonstrated statistically significant differences between cases of xanthogranulomatous cholecystitis and gallbladder cancer. Furthermore, all the twelve patients with xanthogranulomatous cholecystitis exhibited at least one positive computed tomography imaging feature aside from past acute cholecystitis episode, and no patient with advanced gallbladder cancer simultaneously exhibited past acute cholecystitis episode and at least one positive computed tomography imaging feature.</p><p><b>CONCLUSIONS</b>The accurate preoperative diagnosis of xanthogranulomatous cholecystitis includes an integrated review of past acute cholecystitis episode, choledocholithiasis, and positive computed tomography imaging features. Besides, we present an algorithm for intraoperative diagnosis.</p>


Subject(s)
Aged , Aged, 80 and over , Cholecystitis , Diagnosis , Diagnostic Imaging , Female , Gallbladder Neoplasms , Diagnosis , Diagnostic Imaging , Granuloma , Diagnosis , Diagnostic Imaging , Humans , Male , Middle Aged , Radiography , Retrospective Studies , Xanthomatosis , Diagnosis , Diagnostic Imaging
3.
Chinese Medical Journal ; (24): 137-141, 2010.
Article in English | WPRIM | ID: wpr-266011

ABSTRACT

<p><b>BACKGROUND</b>Oddi sphincter plays an important role in preventing reflux cholangitis. There exists the controversy on application of choledochoduodenostomy in hepatolithiasis management. The present study aimed at evaluating long-term outcomes of choledochoduodenostomy for the treatment of hepatolithiasis.</p><p><b>METHODS</b>Forty-six consecutive cases of hepatolithiasis who underwent choledochoduodenostomy were analyzed retrospectively. The pre- and postoperative rates of recurrent cholangitis and acute cholangitis severe type were compared. Paired chi-square test was applied.</p><p><b>RESULTS</b>The mean follow-up time was 17.3 years ranging from 1.6 to 40 years with a follow-up rate of 97.8% (45/46). High rates of remnant stones (39.1%, 18/46), recurrent stones (31.1%, 14/45), uncorrected strictures (85%, 17/20), and mortality (24.4%, 11/45) were observed in this group. Regurgitation of food debris and duodenal content into the biliary tract through the anastomosis was observed. The rate of recurrent cholangitis was equal to the preoperative period (93.3%, 42/45). The rate of acute cholangitis severe type after choledochoduodenostomy (46.7%, 21/45) increased significantly (P<0.01) when compared to the preoperative period (20.0%, 9/45).</p><p><b>CONCLUSIONS</b>Choledochoduodenostomy did not entirely achieve the goal of clearance of stones, correction of strictures, and removing of hepatobiliary lesions by itself. Choledochoduodenostomy without cholangioplasty resulted in an increase of severe reflux cholangitis due to the loss of the anti-reflux function of the sphincter of Oddi. Therefore, choledochoduodenostomy is not an ideal approach to reduce cholangitis in hepatolithiasis and is not the best choice in the management of hepatolithiasis.</p>


Subject(s)
Adolescent , Adult , Aged , Choledochostomy , Female , Humans , Lithiasis , General Surgery , Liver Diseases , General Surgery , Male , Middle Aged , Retrospective Studies , Treatment Outcome , Young Adult
4.
Chinese Journal of Oncology ; (12): 908-910, 2009.
Article in Chinese | WPRIM | ID: wpr-295208

ABSTRACT

<p><b>OBJECTIVE</b>To explore a novel non-invasive method in detection of thyroid cancer by Fourier transform infrared (FTIR) spectroscopy.</p><p><b>METHODS</b>Surface FTIR spectra of 15 cases of thyroid cancer and 51 cases of normal subjects were collected. 22 variables of 11 bands including peak positions and relative intensities were measured and all data were statistically analyzed.</p><p><b>RESULTS</b>In the cancer group: (1) the peak position of 1743 cm(-1) was shifted toward higher wave number (P < 0.05), and that of 1250 cm(-1) to the lower (P < 0.05), when compared to those of normal ones. (2) The relative intensity ratios of I(1546)/I(1460), I(1250)/I(1460), I(1120)/I(1460), I(1080)/I(1460) were significantly increased (P < 0.05). (3) The presence rate of band of 1340 cm(-1) was significantly decreased (P < 0.05).</p><p><b>CONCLUSION</b>FTIR surface spectra may become a novel powerful non-invasive approach of detecting thyroid cancer in regular routine check-up.</p>


Subject(s)
Humans , Spectroscopy, Fourier Transform Infrared , Methods , Thyroid Gland , Chemistry , Thyroid Neoplasms , Chemistry , Diagnosis
5.
Chinese Journal of Surgery ; (12): 1182-1184, 2007.
Article in Chinese | WPRIM | ID: wpr-340835

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the therapeutic effect of subcutaneous tunnel hepaticoplasty on the treatment of hepatolithiasis.</p><p><b>METHODS</b>The early complications and clinical effects of 99 hepatolithiasis cases who underwent subcutaneous tunnel hepaticoplasty from January 1993 to August 2006 were analyzed retrospectively. The stones of 28 (28.3%) patients were in the left lobe, 24.2% (24/99) in the right, and 47.5% (47/99) in bilateral lobe. Sixty-six patients (66.7%) had both stones and biliary strictures. During the procedure, a portion of the liver habouring stone was resected if necessary. The hepatic duct and strictures were opened, the stones were removed, and the porta hepatis was repaired by one end of a segment of jejunum. The other end of the jejunum was set subcutaneously. The gall bladders of 27 patients (27.3%) were used as subcutaneous tunnel instead.</p><p><b>RESULTS</b>Ninety-five out of ninety-nine cases were followed up with an average of 4.2 years (1 month to 13.5 years). The rates of residual stone, recurrent stone and cholangitis were 23.2% (23/99), 20.0% (19/95) and 14.7% (14/95) respectively. Postoperatively, 34 cases who had residual or recurrent stones were underwent lithotomy by choledochoscope through the subcutaneous blind loop and the achievement ratio was 91.2% (31/34).</p><p><b>CONCLUSIONS</b>Subcutaneous tunnel hepatocholangioplasty decreases the relapsing cholangitis effectively, and makes an easy way to take out residual or recurrent stones.</p>


Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Bile Ducts, Intrahepatic , Pathology , General Surgery , Biliary Tract Surgical Procedures , Methods , Cholelithiasis , Pathology , General Surgery , Female , Follow-Up Studies , Hepatectomy , Methods , Humans , Liver Diseases , Pathology , General Surgery , Male , Middle Aged , Postoperative Complications , Recurrence , Retrospective Studies , Treatment Outcome
6.
Chinese Journal of Oncology ; (12): 512-514, 2006.
Article in Chinese | WPRIM | ID: wpr-236946

ABSTRACT

<p><b>OBJECTIVE</b>To identify the FTIR spectroscopic characterization of breast cancer and explore the possibility of application of FTIR in differentiation of malignant and benign breast lesions.</p><p><b>METHODS</b>FTIR spectra of surgically removed fresh breast tissues were measured by spectrometer equipped with mid-infrared fiber optics and an ATR probe. Peaks in the spectra were measured and relative intensity ratios were calculated and analyzed if there are significant differences between the spectra of malignant and benign breast lesions.</p><p><b>RESULTS</b>There were significant differences (P < 0.05) between the spectra of malignant breast cancers and benign breast tissues in the relative intensity ratios of different peaks (I1640/ I1550 and I1160/I1120 for protein structures; I1640/I1460 and I1550/I1460 for relative content of protein and lipid; I1460/I1400 for lipid structures; I1310/I1240 for nucleic acid).</p><p><b>CONCLUSION</b>FTIR spectroscopy could be a useful tool in clinical diagnosis of breast cancer.</p>


Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Breast , Chemistry , Pathology , Breast Neoplasms , Diagnosis , Metabolism , Carcinoma, Ductal, Breast , Diagnosis , Metabolism , Female , Fibroadenoma , Diagnosis , Metabolism , Humans , Lipids , Middle Aged , Neoplasm Proteins , Reproducibility of Results , Sensitivity and Specificity , Spectroscopy, Fourier Transform Infrared , Methods
7.
Chinese Journal of Surgery ; (12): 1620-1623, 2006.
Article in Chinese | WPRIM | ID: wpr-334444

ABSTRACT

<p><b>OBJECTIVE</b>To explore the impact of different treatment procedure on the prognosis of T1 and T2 stage gallbladder cancer with the invasion within the gallbladder wall.</p><p><b>METHODS</b>A retrospective analysis was conducted on 45 patients with pathologic stage T1 and T2 gallbladder cancer who had undergone surgical resection from 1990 and 2005.</p><p><b>RESULTS</b>Depth of invasion (T), radical cholecystectomy and postoperative adjuvant chemotherapy were independent prognostic factors on Cox multivariate analysis. The 5-year survival rates of patients with T1a, T1b and T2 stage gallbladder cancer who underwent simple cholecystectomy without postoperative adjuvant chemotherapy were 100%, 67% and 0, respectively. The survival rate in T2 was significantly lower than those in T1a and T1b. Without postoperative adjuvant chemotherapy, the 5-year survival rates of patients with T2 stage gallbladder cancer who underwent simple cholecystectomy and radical cholecystectomy were 0 and 63%, respectively. There was significant difference between the survival time of T2 patients who had undergone simple cholecystectomy with and without postoperative adjuvant chemotherapy.</p><p><b>CONCLUSIONS</b>The prognosis of patients with T1 stage gallbladder cancer is much better than that of T2 stage. The 5-year survival rates of patients with T1a and T1b stage gallbladder cancer who received simple cholecystectomy are relatively good. Radical cholecystectomy and postoperative adjuvant chemotherapy can improve the prognosis of patients with T2 gallbladder cancer.</p>


Subject(s)
Adult , Aged , Aged, 80 and over , Chemotherapy, Adjuvant , Cholecystectomy , Combined Modality Therapy , Female , Gallbladder , Pathology , General Surgery , Gallbladder Neoplasms , Pathology , Therapeutics , Humans , Male , Middle Aged , Neoplasm Invasiveness , Neoplasm Staging , Prognosis , Retrospective Studies , Survival Analysis
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