Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 53
Filter
Add filters








Year range
1.
Article in Chinese | WPRIM | ID: wpr-877598

ABSTRACT

OBJECTIVE@#To observe the clinical therapeutic effect of electroacupuncture (EA) combined with tamsulosin hydrochloride sustained release capsule on chronic prostatitis (CP) of damp and heat stasis.@*METHODS@#A total of 70 patients with CP of damp and heat stasis were randomized into an acupuncture plus medication group (35 cases, 4 cases dropped off) and a medication group (35 cases, 5 cases dropped off). In the medication group, tamsulosin hydrochloride sustained release capsule was given orally, 0.2 mg a time, once each night. On the basis of treatment in the medication group, EA was applied at Guanyuan (CV 4), Sanyinjiao (SP 6) and Yinglingquan (SP 9), with disperse-dense wave, 5 mA in intensity for 30 min. Treatment for 30 days was as one course, and totally 3 courses were required in both groups. Before treatment, 1, 2, 3 months into treatment and at the follow-up of 2 months after treatment, the TCM syndrome score and National Institutes of Health chronic prostatitis symptom index (NIH-CPSI) score were observed, and the clinical efficacy was evaluated in both groups.@*RESULTS@#Compared before treatment, the TCM syndrome scores of 3 months into treatment and follow-up were decreased in the acupuncture plus medication group (@*CONCLUSION@#Acupuncture combined with medication can improve the clinical symptoms in patients with CP of damp and heat stasis, and its therapeutic effect is superior to simple western medication.


Subject(s)
Acupuncture Points , Acupuncture Therapy , Chronic Disease , Hot Temperature , Humans , Male , Prostatitis/drug therapy , Treatment Outcome
2.
Article in Chinese | WPRIM | ID: wpr-817893

ABSTRACT

Adrenal insufficiency(AI)is a type of adrenocortical hormone-deficient lesion caused by primary or secondary factors. Because of its lack of specificity in clinical manifestations,it is difficult to identify early,which may lead to delayed diagnosis. If the treatment is not timely,it may cause adrenal crisis(AC)or even sudden death. Sudden death in children due to adrenal insufficiency may be directly related to hyperkalemia caused by adrenal crisis. Gastrointestinal illness and infections are common precipitant for an adrenal crisis. Despite it being a treatable condition for now,failure of adequate preventive measures or delayed treatment has often led to unnecessary deaths. Therefore,more measures are needed to prevent the adrenal crisis and to ensure that appropriate emergency medical services are established after the crisis to reduce the rate of sudden death.

3.
Article in Chinese | WPRIM | ID: wpr-699601

ABSTRACT

Objective To observe the retinal thickness of macular in type 2 diabetic mellitus (T2DM) patients without clinical features of diabetic retinopathy (DR).Methods Totally 40 patients (40 eyes) with T2DM without DR and 70 healthy volunteers (70 eyes) from August 2017 to October 2017 were enrolled in this study.Usage of spectral domain optical coherence tomography (SD-OCT) and the software of automatic segmentation to measure the average thickness of total retinal (R),inner retinal layer (IRL) and outer retinal layer namely photoreceptor layer (PL) in macular.The foveal center was divided according to three concentric circle with the diameter of 1 mm,3 mm and 6 mm (including the partition of R total,R-1,R-3,R-6,IRL-1,IRL-3,IRL-6,PL-1,PL-3 and PL-6),and the average retinal thicknesses of these partitions between these two group were compared and analyzed.Results The thickness of PL-1 and PL-3 in no DR group was significantly thinner than that in the normal control group [(71 ± 4)μm vs.(73 ± 3) μm and (66 ± 2) μm vs.(67 ± 2) μm,respectively] (both P < 0.05).In the normal control group,except the IRL-6 and PL-1,the difference of the thickness was significant in the other macular regions between various genders (all P < 0.05).In the male subjects,the thickness of PL-3 and PL-6 in no DR group was significantly thinner than that in the normal control group [(67 ± 2) μm vs.(68 ± 2) μm and (65 ± 2) μm vs.(66 ± 2) μm,respectively] (both P < 0.05).In the female subjects,the mean thicknesses of PL-3 and PL-6 in no DR group was significant thinner than those in normal control group [(65 ± 2) μm vs.(67 ± 2) μm and (63 ± 2) μm vs.(64 ± 2) μm,respectively] (both P < 0.05).There was no obviously difference in the other parts between these two groups,Conclusion The mean retinal thicknesses of the parafovea and perifovea are significantly thinner in no DR group than that of the normal control group.The measurement of the PL thickness of macular by SD-OCT may promote the study of early stage of DR,and become an important biological marker for early monitoring of DR.

4.
Article in Chinese | WPRIM | ID: wpr-696199

ABSTRACT

Objective To explore the correlations of neutrophil gelatinase associated lipocalin (NGAL) with cerebral stroke attack and renal dysfunction of patients.Methods 63 patients with first-diagnosed hemorrhagic stroke (HS),233 patients with first diagnosed ischemic stroke (IS) and 293 healthy controls were randomly selected.Their age,gender and clinical his tory of hypertension,diabetes,coronary heart disease and hyperuricemia were recorded at admission,and their serum levels of kidney function/injury biomarkers (NGAL,Cr,Urea,CysC and eGFR) were measured at the next day.According to KDIGO guidelines standards,all subjects were divided into four groups on behalf of their renal damage degree:AKI,CKD,transient or no kidney injury,and control group.And then,the relations of these indicators with stroke attack and renal function of HS and IS patients were analyzed.Results The medians of serum NGAL levels in the patients with HS and IS were 160.0 and 142.9 μg/L,their difference had no statistical significance (Z=2.332,P>0.05),but were higher than healthy controls (Z =12.621,9.189,all P<0.05).The levels of serum NGAL in AKI,CKD,transient or no renal dysfunction,and control groups were 375.3,228.6,141.6 and 103.8 μg/L respectively,which had statistically significant differences between each other (Z=3.661~11.237,all P<0.05).NGAL level was positively related to HS attack (rp=0.423,P<0.001),IS attack (rp =0.231,P<0.001) and renal dysfunction in both HS and IS patients (rp=0.429,0.289,all P<0.001).Its partial cor relation coefficient was only lower than hypertension within HS stroke attack,and lower than coronary heart disease and diabetes within IS stroke attack,but the highest within renal function of either HS or IS patients.For assessing HS and IS attack,NGAL levels play a limited role (OR=1.044,0.974,allP<0.001),in contrary to NGAL abnormity (OR =27.841,15.411,all P<0.001).Conclusion Elevated NGAL levels may be a risk factor for stroke,suggesting that kidney injury or renal dysfunction may be associated with stroke,and the prevention and treatment of stroke should pay attention to the change of NGAL.

5.
Article in Chinese | WPRIM | ID: wpr-712923

ABSTRACT

[Objective]To construct miR-18a overexpression and inhibition lentivirus vectors and to determine their effects on human nasopharyngeal cancer(NPC)cell line CNE1 and CNE2.[Methods]Designed the primers for Real-time polymerase chain(PCR)reaction to obtain the miR-18a premature gene.The premature gene and the siRNA oligo-nucleutides of miR-18a were connected to the lentivirus vector GV369 and GV280,respectively.The construction vectors were confirmed by DNA sequencing.Then,293T cell was infected with the vectors plus Helper 1.0 and pHelper 2.0 vec-tors to obtain recombinant lentivirus vector for miR-18a overexpression and inhibition. The NPC cell line CNE1 and CNE2 were infected with the successful recombinant lentivirus vectors.Puromycin was added to select the positive infect-ed cells. PCR method was used to detect the miR-18a expression level after infecting the recombinant lentivirus vector into the NPC cell line.[Results]A recombinant lentivirus vector expressing miR-18a interference oligonucleutides was obtained and confirmed by DNA sequencing.The virus titer was 3×108TU/mL,and the expression of its target gene ATM was downregulated in CNE1 and CNE2.A recombinant lentivirus vector expressing miR-18a premature gene was obtained and confirmed by DNA sequencing. The virus titer was 3×108TU/mL,and the miR-18a was overexpressed in CNE1 (20.3 fold upregulation,P<0.01)and CNE2(122.5 fold upregulation,P<0.01),and its target gene ATM was downregu-lated.[Conclusions]The miR-18a overexpression and suppression lentivirus vectors are successfully constructed.These vec-tors could alter the expression level of miR-18a in NPC cell line significantly,and provide a stable cell line for functional studies in the future.

6.
Article in Chinese | WPRIM | ID: wpr-776688

ABSTRACT

A boy aged 6 years and 3 months developed upper respiratory tract infection and pyrexia 2 months ago and was given oral administration of nimesulide by his parents according to directions. Half an hour later, the boy experienced convulsions and cardiopulmonary arrest, and emergency examination found hypoketotic hypoglycemia, metabolic acidosis, significant increases in serum aminotransferases and creatine kinase, and renal damage. Recovery of consciousness and vital signs was achieved after cardiopulmonary resuscitation, but severe mental and movement regression was observed. The boy had a significant reduction in free carnitine in blood and significant increases in medium- and long-chain fatty acyl carnitine, urinary glutaric acid, 3-hydroxy glutaric acid, isovalerylglycine, and ethylmalonic acid, suggesting the possibility of multiple acyl-CoA dehydrogenase deficiency. After the treatment with vitamin B2, L-carnitine, and bezafibrate, the boy gradually improved, and reexamination after 3 months showed normal biochemical parameters. The boy had compound heterozygous mutations in the ETFDH gene, i.e., a known mutation, c.341G>A (p.R114H), from his mother and a novel mutation, c.1484C>G (p.P495R), from his father. Finally, he was diagnosed with multiple acyl-CoA dehydrogenase deficiency. Reye syndrome and sudden death symptoms were caused by nimesulide-induced acute metabolic crisis. It is concluded that inherited metabolic diseases may be main causes of Reye syndrome and sudden death, and biochemical and genetic analyses are the key to identifying underlying diseases.


Subject(s)
Acyl-CoA Dehydrogenase , Administration, Oral , Carnitine , Child , Death, Sudden , Humans , Male , Respiratory Tract Infections , Reye Syndrome , Sulfonamides
7.
China Journal of Endoscopy ; (12): 23-27, 2018.
Article in Chinese | WPRIM | ID: wpr-702878

ABSTRACT

Objective To explore the optimal bowel preparation for capsule endoscopy (CE). Methods 102 patients were recruited for CE and randomly divided into 3 groups. The group A (n = 40) : patients received polyethylene glycol electrolyte powder (PEG) 137.12 g dissolved in 2 000 ml water at 21:00 one day prior to CE, and taken PEG 68.56 g dissolved in 1 000 ml of water four hours before the procedure. Group B (n = 32): patients received PEG 205.68 g dissolved in 3 000 ml of water four hours prior to CE. Group C (n = 30): patients used a 500 ml 20% mannitol and 2 500 ml water bowel preparation four hours prior to CE. All patients were treated with 120 mg simethicone immediately after swallowing CE. The incidences of adverse events, small-bowel preparation quality and transit time were analyzed. Results The adverse effects rate in each group was similar (15.00%, 15.63% vs 16.67%, P > 0.05). The small-bowel preparation quality was better in both B and C groups than A group (P < 0.05). In the C Group, small-bowel preparation quality was slightly better than the B group, but the difference was not statistically significant (P > 0.05). In comparison with patients in both B and C groups, those in A group had a longer small-bowel transit time (P < 0.05), whereas there was no significant difference between B and C group (P > 0.05). Conclusion Bowel cleansing effect was better in single dose regimen than split dose protocol. The single dose regimen of 500 ml 20% Mannitol for bowel preparation is suitable prior to CE.

8.
Article in Chinese | WPRIM | ID: wpr-351382

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of atopy on the expression of glucocorticoid receptors in children with bronchiolitis.</p><p><b>METHODS</b>ELISA was used to measure the changes in the serum levels of glucocorticoid receptor α (GRα) and glucocorticoid receptor β (GRβ) in the bronchiolitis group (77 children, including 34 children with atopy) and pneumonia group (68 children). Thirty-eight children who were prepared to undergo surgeries for non-infectious diseases and had no atopy or family history of allergic diseases were enrolled as the control group.</p><p><b>RESULTS</b>The bronchiolitis group and the pneumonia group had significant increases in the serum levels of GRα and GRβ compared with the control group (P<0.01), and the bronchiolitis group had significant increases in these levels compared with the pneumonia group (P<0.01). Compared with the control group and the pneumonia group, the bronchiolitis group had a significant increase in the GRα/GRβ ratio (P<0.01). Compared with the control group, the children with or without atopy in the bronchiolitis group had significant increases in the serum levels of GRα and GRβ (P<0.01). The non-atopic children in the bronchiolitis group had a significant increase in the serum level of GRβ compared with the atopic children (P<0.01). The atopic children in the bronchiolitis group had a significant increase in the GRα/GRβ ratio compared with the control group and non-atopic children in the bronchiolitis group (P<0.01).</p><p><b>CONCLUSIONS</b>Children with bronchiolitis have increased serum levels of GRα and GRβ. The children with atopy have an increased GRα/GRβ ratio, suggesting that the atopic children with bronchiolitis are highly sensitive to glucocorticoids.</p>


Subject(s)
Bronchiolitis , Blood , Female , Humans , Hypersensitivity , Blood , Infant , Male , Receptors, Glucocorticoid , Blood
9.
Article in Chinese | WPRIM | ID: wpr-360144

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the value of high-frequency echocardiography in assessing cardiac structure and function in a mouse model of myocardial infarction.</p><p><b>METHODS</b>Twenty-five C57BL/6 mice were randomly divided into sham-operated group (n=10) and myocardial infarction model group (n=15) established by ligation of the left anterior descending artery. The cardiac structure, regional wall motion and cardiac function of mice were examined with pulsed wave Doppler (PWD), tissue Doppler imaging (TDI), EKV and M-mode echocardiography 3 days before and at 1 week after the operation. The histological changes and myocardial structure of the heart were observed at 1 week after the operation.</p><p><b>RESULTS</b>High-frequency echocardiography and HE staining detected obvious myocardial infarction in the mice in the model group. Compared with the sham-operated mice, the mice with myocardial infarction showed significant left ventricular expansion, obvious thinning of the ventricular wall, and significantly decreased ventricular systolic function and diastolic function with regional wall motion abnormality and ventricular remodeling.</p><p><b>CONCLUSION</b>s 2D-type echocardiography combined with M-mode, PWD, TDI and EKVTM for allows accurate and sensitive detection of the loci and severity of myocardial infarction to provide important evidence for clinical diagnosis and treatment of myocardial infarction.</p>

10.
Chinese Journal of Zoonoses ; (12): 720-723,729, 2017.
Article in Chinese | WPRIM | ID: wpr-703034

ABSTRACT

To evaluate the clinical value of gene chip technology (GCT) in detecting the mycoba-cteria,isoniazid and rifampin resistance of patients diagnosed tuberculous empyema.The 182 patients who met the inclusion criteria were enrolled to this study from January 2011 to December 2015,whose pus mycobacterial species were detected by GCT and MGIT,the simultaneous and sensitivity of them were compared.Meanwhile,36 patients diagnosed tuberculous empyema were selected to detect isoniazid and rifampin resistance.The simultaneous and sensitivity of GCT were evaluated base on the standard of MGIT.The 135 patients were diagnosed by tuberculous empyema.The specificity of GCT was same to MGIT (95.7%),the the sensitivity was 48.9% (66/135)in GCT,26.7% in MGIT,there was significant difference between them (x2=80.5,P< 0.05).The sensitivity,specificity and coincidence rate of GCT in rifampin resistance were 100%,the sensitivity,specificityand coincidence rate in INH were 50.0%(1/2),97.1%(33/34) and 94.4%.Gene chip technology for detection of mycobacteria has high sensitivity and specificity,which can identify non-tuberculous mycobacteria quickly.And it can also effectively detect the resistance of Mycobacterium tuberculosis to isoniazid and rifampicin.It has important significance in early diagnosis and treatment of tuberculous empyema.

11.
Journal of Experimental Hematology ; (6): 1833-1836, 2016.
Article in Chinese | WPRIM | ID: wpr-332602

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the role and significance of T help cells 17(Th17) in pathogenesis of idiopathic thrombocytopenic purpura (ITP).</p><p><b>METHODS</b>Peripheral blood samples from ITP patients and normal controls were examined for Th17 cell proportion by flow cytometry (FCM). Expression of IL-17, IL-23, IL-6 and TGF-β1 in hematoplasma was detected by ELISA. The mRNA expression level of IL-17 and RORγt in peripheral blood mononuclear cells (PBMNC) from patients with ITP and normal controls were measured by RT-PCR technique, and expression levels of pSTAT3 and RORγt proteins were analyzed by Western-blot.</p><p><b>RESULTS</b>Th17 cells in peripheral blood from patients with ITP was greatly increased when compared with normal control group (P<0.05). Expressions of IL-17, IL-23, IL-6 and TGF-β1 in hematoplasma of ITP patients were all significantly higher than those in normal control group (all P<0.01). mRNA expression levels of IL-17 and RORγt in PBMNC from patients with ITP were much higher than those in normal controls (P<0.05). Protein expressions of pSTAT3 and RORγt in PBMNC of ITP patients were greatly increased as compared with those in control (P<0.05).</p><p><b>CONCLUSION</b>Th17 cell subgroup may play a role in incidence and development of ITP, which may participate in the pathogenesis of ITP by increasing Th17 cell proportion and altering the expression level of Th17-related cytokines as well as regulatory and transcriptional factors.</p>

12.
Article in Chinese | WPRIM | ID: wpr-261215

ABSTRACT

A one-year-old girl visited the hospital due to limb torsion and developmental regression for one month after hand, foot and mouth disease. At the age of 11 months, she visited a local hospital due to fever for 5 days and skin rash with frequent convulsions for 2 days and was diagnosed with severe hand, foot and mouth disease, viral encephalitis, and status epilepticus. Brain MRI revealed symmetric abnormal signals in the bilateral basal ganglia, bilateral thalamus, cerebral peduncle, bilateral cortex, and hippocampus. She was given immunoglobulin, antiviral drugs, and anticonvulsant drugs for 2 weeks, and the effect was poor. Blood and urine screening for inherited metabolic diseases were performed to clarify the etiology. The analysis of urine organic acids showed significant increases in glutaric acid and 3-hydroxyglutaric acid, which suggested glutaric aciduria type 1, but her blood glutarylcarnitine was normal, and free carnitine significantly decreased. After the treatment with low-lysine diets, L-carnitine, and baclofen for 1 month, the patient showed a significant improvement in symptoms. Hand, foot and mouth disease is a common viral infectious disease in children, and children with underlying diseases such as inherited metabolic diseases and immunodeficiency may experience serious complications. For children with hand, foot and mouth disease and unexplained encephalopathy, inherited metabolic diseases should be considered.


Subject(s)
Amino Acid Metabolism, Inborn Errors , Brain Diseases, Metabolic , Developmental Disabilities , Female , Glutaryl-CoA Dehydrogenase , Hand, Foot and Mouth Disease , Humans , Infant , Torsion Abnormality
13.
Article in English | WPRIM | ID: wpr-44146

ABSTRACT

A 48-year-old woman presented with a 50-day history of irregular vaginal bleeding and lower abdominal pain. Ultrasound indicated an extremely large occupying lesion in the pelvic cavity that was highly suggestive of malignancy. Fluorodeoxyglucose (FDG) positron emission tomography/computed tomography (PET/CT) was performed to further assess the nature of pelvic abnormality. PET/CT images demonstrated a diffusely lobulated mass ranging from cervix up to the inferior pole of kidneys with mild FDG uptake. Simultaneously, multiple nodules in bilateral lungs and a hypodense lesion in the right ventricle were shown without FDG-avidity. Based on the imaging results, the presumptive diagnosis was uterine intravenous leiomyomatosis with intracardiac extension and pulmonary benign metastases, which was subsequently confirmed by MRI and the lesion biopsy.


Subject(s)
Female , Fluorodeoxyglucose F18/chemistry , Humans , Leiomyoma/pathology , Leiomyomatosis/pathology , Lung Neoplasms/diagnostic imaging , Magnetic Resonance Imaging , Middle Aged , Positron-Emission Tomography , Tomography, X-Ray Computed , Uterine Neoplasms/pathology , Vena Cava, Inferior/pathology
14.
International Eye Science ; (12): 55-57, 2015.
Article in Chinese | WPRIM | ID: wpr-637008

ABSTRACT

?Proliferative diabetic retinopathy ( PDR ) is a group of disease characterized by neovascular disease complication of diabetes mellitus. Neovascular diseases of eye are one of the major causes of blindness of the world. Recent studies showed that vascular endothelial growth factor ( VEGF) and pigment epithelium-derived factor ( PEDF ) are now accepted as the key cytokine in the development of diabetic retinopathy. Recent progress in the investigation of VEGF and PEDF of PDR are summarized in this review.

15.
Article in Chinese | WPRIM | ID: wpr-312786

ABSTRACT

<p><b>OBJECTIVE</b>To observe the clinical efficacy of treating chronic persistent bronchial asthma (CPBA) children with abnormal myocardial enzyme spectrum (AMES) by Yupingfeng Powder (YP) combined routine therapy.</p><p><b>METHODS</b>From January 2010 to December 2012, 156 CPBA children patients with AMES were randomly assigned to the treatment group (80 cases) and the control group (76 cases). All patients received routine treatment (inhaled corticosteroids and/or leukotriene regulator). Besides, those in the treatment group took YP. The treatment duration was 3 months. The scores of children asthma control test (C-ACT), pulmonary function (FEV,% and PEF%), myocardial enzyme spectrum were observed before and after treatment, and 3 months before and after treatment. The myocardial enzyme spectrum of 40 healthy children at the baby clinics during the same period were recruited as the control.</p><p><b>RESULTS</b>Compared with the control group, creatine kinase isoenzyme (CK-MB), creatine kinase(CK), and lactate dehydrogenase (LDH) increased in the two treatment groups (P <0.01), but there was no statistical difference in AST (P >0.05). Compared with before treatment in the same group, CK-MB, CK, LDH, and AST decreased in the treatment group after treatment and 3 months after treatment (P <0.01). CK-MB, CK, LDH, and AST decreased in the control group 3 months after treatment (P <0.01, P <0.05).Compared with after treatment, CK decreased in the control group 3 months after treatment (P <0.01). C-ACT score, FEV(1),%, and PEF% all increased in the two groups after treatment and 3 months after treatment (P <0.01, P <0.05). Compared with after treatment in the same group, CK decreased in the control group 3 months after treatment (P <0. 01). Compared with the control group in the same period, post-treatment CK-MB and CK decreased (P <0. 01, P <0. 05), while post-treatment C-ACT score, FEV, %, and PEF% increased (P <0.05) in the treatment group (P <0.05).</p><p><b>CONCLUSION</b>YP could strengthen specific and non-specific immunity of the organism, and improve clinical symptoms and the level of myocardial enzyme spectrum.</p>


Subject(s)
Asthma , Therapeutics , Child , Chronic Disease , Therapeutics , Creatine Kinase, MB Form , Metabolism , Drugs, Chinese Herbal , Therapeutic Uses , Humans , L-Lactate Dehydrogenase , Metabolism , Myocardium
16.
Article in Chinese | WPRIM | ID: wpr-337222

ABSTRACT

<p><b>OBJECTIVE</b>To compare efficacy differences between fire filiform needle combined with mild moxibustion and gabapentin combined with sham acupuncture for postherpetic neuralgia (PHN).</p><p><b>METHODS</b>One hundred cases of PHN were randomly divided into a needle group and a medicine group, 50 cases in each one. In the needle group, pricking method of fire filiform needle was given at the Ashi points, and then mild moxibustion was applied for 15 min. In the medicine group, the oral administration of gabapentin capsule and sham acupuncture at non-acupoints in the distal end of lesions were applied. The treatment was required for 21 days in both groups. The visual analogue score (VAS) was recorded before treatment and on the 1st day, 2nd day, 3rd day, 6th day, 9th day and 12th day of treatment. The most severity of pain within last 24 h, preset severity of pain, immediate analgesia effect and starting time of pain relief were observed, also the efficacy was assessed and improvement of symptoms was observed in the follow-up visit.</p><p><b>RESULTS</b>The total effective rate was 94.0% (47/50) in the fire filiform needle group, which was superior to 86.0% (43/50) in the medicine group (P < 0.05). Compared with medicine group, the VAS of the most severity of pain within last 24 h was obviously reduced after the 2nd treatment in the fire filiform needle group while that of present severity of pain was relieved after the 1st treatment (both P < 0.05). The immediate analgesia effect in the fire filiform needle group was obviously superior to that in the medicine group in the first three times of treatment (all P < 0.05). The average time of pain relief was (3.91 +/- 0.82) days in the fire filiform needle group, which was significantly earlier to (6.53 +/- 1.13) days in the medicine group (P < 0.05). 26 cases were cured in the fire filiform needle group in the follow-up visit, which was superior to 2 cases in the medicine group (P < 0.05). The improvement of VAS, pain range and sleep quality in the needle group were also superior to those in the medicine group (all P < 0.05). The direct medical cost in the fire filiform needle group was (232.32 +/- 48.108) yuan, which was significantly lower than (466.00 +/- 41.09) yuan in the medicine group (P < 0.05). There was only one case of adverse effect in the medicine group during the treatment.</p><p><b>CONCLUSION</b>The fire filiform needle combined with mild moxibustion could obviously relieve the pain in PHN patients, which has superior immediate analgesia effect and pain relieving time compared with gabapentin, which also has less adverse effects and cheap cost.</p>


Subject(s)
Acupuncture Points , Acupuncture Therapy , Aged , Combined Modality Therapy , Female , Humans , Male , Middle Aged , Moxibustion , Neuralgia, Postherpetic , Therapeutics , Pain Measurement , Treatment Outcome
17.
Article in English | WPRIM | ID: wpr-293300

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects of panax notoginseng saponins (PNS) on expression, regulation and phosphorylation of multiple protein kinases in mitogen activated protein kinase (MAPK) intracellular signal pathway and GATA transcription factors in hematopoietic cells, so as to explore its mechanism of proliferation and differentiation activity on hematopoiesis.</p><p><b>METHODS</b>The human granulocytic HL-60, erythrocytic K562, megakaryocytic CHRF-288 and Meg-01 cell lines were treated by PNS, the positive control of K562, CHRF-288 cells treated by recombination human erythropoietin (Epo) and thrombopoietin (Tpo) respectively. The total cell lysate and nuclei protein were extracted after being treated by PNS, subsequently, analyzed by both Western blot and immune-precipitation. Meanwhile, the nuclei extract was performed for electrophoretic mobility shift assay (EMSA) by using (32)P radio labeled double-stranded GATA consensus oligonucleotide.</p><p><b>RESULTS</b>The expression levels of kinase MEK-1, MEK-2, ERK-1, ERK-2, AKT-1, AKT-2 and PI-3K were increased by PNS treatment to different extent in four cell lines, depending on cellular heterogeneity and sensitivity to PNS, also phosphorylation of MEK-1, ERK-1 was differentially promoted by PNS respectively P<0.05, 0.01, 0.001). The expression levels of transcription factors GATA-1 and GATA-2 were increased, moreover, their DNA binding activities were raised dramatically in PNS treated K562, CHRF-288 and Meg-01 cells compared with the controls respectively (P<0.05, 0.01, 0.001). The positive control of K562, CHRF-288 cells treated by Epo or Tpo respectively also displayed up-regulation of protein kinases and GATA transcription factors respectively (P<0.05, 0.01, 0.001).</p><p><b>CONCLUSION</b>The results indicated that intracellular signal pathway initiated by PNS was involved in MAPK pathway and transcription factors of GATA family in hematopoietic cells. PNS displayed the role to promote proliferation and differentiation, by means of increasing expression level and phosphorylation status of multiple protein kinases, also inducing synthesis of GATA transcription factors and upregulation its DNA binding activity.</p>


Subject(s)
Blotting, Western , Cell Line, Tumor , DNA , Metabolism , Electrophoretic Mobility Shift Assay , Extracellular Signal-Regulated MAP Kinases , Metabolism , GATA Transcription Factors , Metabolism , Hematopoietic Stem Cells , Humans , Immunoprecipitation , Mitogen-Activated Protein Kinase Kinases , Metabolism , Panax notoginseng , Chemistry , Phosphatidylinositol 3-Kinases , Metabolism , Phosphorylation , Protein Binding , Protein Kinases , Metabolism , Proto-Oncogene Proteins c-akt , Metabolism , Saponins , Pharmacology , Up-Regulation
18.
Article in Chinese | WPRIM | ID: wpr-301436

ABSTRACT

<p><b>OBJECTIVE</b>To study microRNAs (miRNAs) expression profiles associated with epithelial-mesenchymal transition (EMT) in lymph node metastasis of supraglottic laryngeal squamous cell carcinomas(SGLSCC).</p><p><b>METHODS</b>Primary tumor tissue samples of 12 SGLSCC patients were collected, including 6 patients clinically diagnosed with lymph nodes metastasis (N(+)) and 6 patients with lymph nodes metastasis-free (N0), for miRNA microarray gene-expression profiling to identify the differences between N(+) and N0 groups. Differentially expressed miRNAs was verified using quantitative real-time PCR in 20 patients with N(+) and 20 patients with N0. Target genes for the miRNAs associated with EMT in SGLSCC metastasis were analyzed.</p><p><b>RESULTS</b>Ten miRNAs differentially expressed between N(+) group and N0 group were determined. Comparing with N0 group, nine miRNAs were over-expressed and one miRNA was expressed at lower level in N(+) group. The genes for miR-192, miR-143, miR-409 and miR-634 were predicted as target genes that could promote EMT of laryngeal cancer cells by targeted inhibiting Krüppel-like factor 17(KLF17), E-cadherin and phosphatidylinositol 3 kinase (PI3K).</p><p><b>CONCLUSIONS</b>The miRNAs over-expressed in group N(+) can be used to predict cervical lymph node metastasis in SGLSCC. The miRNAs as new markers could improve the diagnosis and treatment of SGLSCC.</p>


Subject(s)
Aged , Cadherins , Carcinoma, Squamous Cell , Genetics , Metabolism , Epithelial-Mesenchymal Transition , Physiology , Gene Expression Profiling , Head and Neck Neoplasms , Genetics , Metabolism , Humans , Laryngeal Neoplasms , Genetics , Metabolism , Larynx , Larynx, Artificial , Lymph Nodes , Lymphatic Metastasis , Genetics , MicroRNAs , Metabolism , Phosphatidylinositol 3-Kinases , Metabolism
19.
Article in Chinese | WPRIM | ID: wpr-345664

ABSTRACT

<p><b>OBJECTIVE</b>To study the variation and clinical significance of serum levels of surfactant proteins A (SP-A) and D (SP-D) among children with different degrees of bronchiolitis.</p><p><b>METHODS</b>Seventy children with bronchiolitis were divided into acute (n=42) and recovery phase groups (n=28). According to the severity of symptoms, the acute phase group was further divided into severe (n=12) and mild subgroups (n=30). Another 26 children who were hospitalized in the same period due to non-infectious diseases and had not undergone surgery were used as the control group. Competitive enzyme-linked immunosorbent assay was performed to measure serum levels of SP-A and SP-D in each group.</p><p><b>RESULTS</b>The acute phase group had significantly higher serum levels of SP-A and SP-D compared with the recovery phase (P<0.01) and control groups (P<0.01). Compared with the control group, the recovery phase group had elevated levels of SP-A and SP-D (P<0.01). Within the acute phase group, serum levels of SP-A and SP-D in the severe subgroup were significantly higher than in the mild subgroup (P<0.01).</p><p><b>CONCLUSIONS</b>Serum levels of SP-A and SP-D are significantly elevated in children with acute bronchiolitis, and severe cases have higher serum levels of SP-A and SP-D than mild cases. Even after the relief of clinical symptoms, serum levels of SP-A and SP-D remain high. These findings suggest that serum levels of SP-A and SP-D might be useful biomarkers for evaluating the severity of bronchiolitis among children.</p>


Subject(s)
Acute Disease , Biomarkers , Bronchiolitis , Blood , Female , Humans , Infant , Male , Pulmonary Surfactant-Associated Protein A , Blood , Pulmonary Surfactant-Associated Protein D , Blood , Severity of Illness Index
20.
Article in English | WPRIM | ID: wpr-347113

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects of sodium copper chlorophyllin (SCC) on the proliferation, differentiation and immunomodulatory function of mesenchymal stem cells (MSCs) from mice with aplastic anemia.</p><p><b>METHODS</b>A mouse model of aplastic anemia was established by exposure of BALB/c mice to sublethal doses of 5.0 Gy Co60 γ radiation, followed by transplantation of 2×10(6) lymph node cells from DBA/2 donor mice within 4 h after radiation. Aplastic anemic BALB/c mice were randomly divided into six groups: the treated groups, which received 25, 50, or 100 mg/kg/day SCC, respectively; a positive control group treated with cyclosporine A (CsA); and an untreated model control group (model group); while, the non-irradiated mice as the normal control group. SCC or CsA were administered by gastrogavage for 20 days, starting on day 4 after irradiation. Peripheral blood cells were counted and colony-forming fibroblasts (CFU-F) in the bone marrow were assayed. The ability of MSCs to form calcium nodes after culture in osteoinductive medium was also observed. The immunosuppressive effect of MSCs on T lymphocytes was analyzed by enzyme-linked immunosorbent assay and flow cytometry, to evaluate the efficacy of SCC in mice with aplastic anemia.</p><p><b>RESULTS</b>Peripheral blood white cell and platelet counts were increased by medium and high SCC doses, compared with the untreated control. CFU-Fs were also increased compared with the untreated control, and the numbers of calcium nodes in MSCs in osteoinductive medium were elevated in response to SCC treatment. The percentage of Forkhead box protein 3 (FOXP3(+)) T cells was increased in T cell-MSC cocultures, and the cytokine transforming growth factor β1 was up-regulated in SCC-treated groups.</p><p><b>CONCLUSION</b>The results of this study suggest that SCC not only promotes the proliferation and differentiation of MSCs, but also improves their immunoregulatory capacity in mice with aplastic anemia.</p>


Subject(s)
Anemia, Aplastic , Blood , Pathology , Therapeutics , Animals , Anthraquinones , Metabolism , Biomarkers , Metabolism , Bone Marrow Cells , Pathology , Calcium , Metabolism , Cell Differentiation , Cell Proliferation , Chlorophyllides , Pharmacology , Colony-Forming Units Assay , Female , Immunosuppression , Leukocyte Count , Male , Mesenchymal Stem Cell Transplantation , Mesenchymal Stem Cells , Cell Biology , Metabolism , Mice , Mice, Inbred BALB C , Mice, Inbred DBA , Osteoblasts , Pathology , Platelet Count , T-Lymphocytes
SELECTION OF CITATIONS
SEARCH DETAIL